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https://www.readbyqxmd.com/read/28820964/modulating-the-modulator-regulation-of-protein-methylation-by-nitric-oxide
#1
Lucas Frungillo, Steven H Spoel
Protein methylation is an important modulator of signal transduction pathways, but methyltransferases themselves may also be modulated. Hu et al. (2017) demonstrate in this issue of Molecular Cell that S-nitrosylation selectively modulates enzymatic activity of a protein arginine methyltransferase vital to abiotic stress tolerance.
August 17, 2017: Molecular Cell
https://www.readbyqxmd.com/read/28820962/wanted-alive-finding-bacteria-in-your-food
#2
Akira Kawamura
In this issue of Cell Chemical Biology, Pezacki and co-workers (Sherratt et al., 2017) report a simple method to metabolically label viable bacteria, which can be used to detect and capture foodborne pathogens. The method may also find many other applications because it can be used to recover live cells, pathogens and non-pathogens, from various biomedical and environmental samples.
August 17, 2017: Cell Chemical Biology
https://www.readbyqxmd.com/read/28820961/optimized-streptavidin-for-fluorescent-labeling-of-biotinylated-targets
#3
Vesa P Hytönen
In order to develop a streptavidin-biotin system with optimal performance in fluorescent labeling, in this issue of Cell Chemical Biology, Jacobsen et al. (2017) focused on changing the surface density of amino groups present on streptavidin via lysine mutagenesis. The streptavidin mutant containing only one free amino group was found superior to other streptavidin variants and was named Flavidin for fluorophore-friendly streptavidin.
August 17, 2017: Cell Chemical Biology
https://www.readbyqxmd.com/read/28820947/dysregulated-mitogen-activated-protein-kinase-and-matrix-metalloproteinase-in-ethanol-induced-cavernosal-dysfunction
#4
Jaqueline Jóice Muniz, Leticia Nogueira Leite, Riccardo Lacchini, Jose E Tanus-Santos, Carlos Renato Tirapelli
We evaluated the effects of ethanol consumption on the mitogen-activated protein kinases (MAPK) and metalloproteinases (MMP) pathways in the rat cavernosal smooth muscle (CSM). Male Wistar rats were treated with ethanol (20% vol./vol.) for six weeks. Quantitative real-time polymerase chain reaction experiments showed that ethanol consumption did not alter mRNA levels of p38MAPK, SAPK/JNK, ERK1/2, MMP-2 or MMP-9 in the rat CSM. Western immunoblotting experiments revealed decreased protein expression of p38MAPK and phosphorylation of SAPK/JNK in the CSM from ethanol-treated rats...
August 18, 2017: Canadian Journal of Physiology and Pharmacology
https://www.readbyqxmd.com/read/28820807/reply-to-dr-neal-et-al
#5
Melissa M Masaracchia, Michael D Herrick, Ellen A Seiffert, Brian D Sites
No abstract text is available yet for this article.
September 2017: Regional Anesthesia and Pain Medicine
https://www.readbyqxmd.com/read/28820701/the-sustainable-development-goals-for-water-the-need-to-consider-perception-preference-and-safety
#6
Christine Stauber
In this month's issue, Brooks et al.(1) examine the role of organoleptic perception and its association with bacteriologic drinking water quality in the Nyanza Province of western Kenya. This study focuses on two important issues relevant for drinking water in the new Sustainable Development Goals (SDGs)(2): human sensory perception and the measures of water safety, and the need to be able to measure drinking water quality in resource limited settings. To address the lack of studies, the authors researched how household members' perception of taste, odor, or overall quality were linked to measured levels of Escherichia coli via two detection methods: IDEXX Colilert(™) Quantitray (Colilert) and the Compartment Bag Test (CBT)...
August 14, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28820520/response-to-assessing-the-impact-of-acupuncture-on-pain-nausea-anxiety-and-coping-in-women-undergoing-a-mastectomy
#7
Jose M Moran, Antonio S Fernández, Juan D Pedrera-Zamorano
We read with interest the pilot randomized, controlled trial by Quinlan-Woodward et al. (2016) published in Oncology Nursing Forum about the impact of acupuncture on pain, nausea, anxiety, and coping in women undergoing a mastectomy. We think that there are both theoretical and methodologic issues that do not allow the authors to conclude that their study demonstrated that acupuncture delivered postoperatively in the hospital after mastectomy can reduce the severity of symptoms experienced and increase the patient's ability to cope with her symptoms...
September 1, 2017: Oncology Nursing Forum
https://www.readbyqxmd.com/read/28820408/-thrombophilic-genetic-mutations-and-polymorphisms-in-women-with-infertility-and-failed-in-vitro-fertilization
#8
G Abrahamyan
Occurrence of pregnancy after in vitro fertilization depends of two components: functional adequacy of the embryo at the blastocyst stage and receptivity of endometrium, which, according to modern perception, are determinate in achieving optimal conditions of implantation. From the pregnancy occurrence point of view, as well as in regard to its further development , implantation is the most crucial phase of IVF/ICSI and ET. As the same time, this phase is also the most vulnerable. Multiple researches have proven the role of mother thrombophilia for genesis of gestation complications and early embryo losses, but in relation to this problem i the context of IVF there is still a lot to be detailed...
July 2017: Georgian Medical News
https://www.readbyqxmd.com/read/28820406/-results-of-preparation-and-implementation-of-ivf-program-in-patients-with-thrombophilia-and-history-of-failed-ivf
#9
G Abrahamyan
The problem of infertility and reproductive losses maintains its urgency, as well as medical and social significance. Frequency of infertility in overall population, according to the data from different authors, varies from 9 to 18 per cent. Methods of aided reproductive technologies (ART) opened a new era in the field of correction of infertile marriage. As a result, more and more couples choose to solve this problem by means of aided reproductive technologies (ART): in-vitro fertilization (IVF) and embryo transfer (ET)...
July 2017: Georgian Medical News
https://www.readbyqxmd.com/read/28820325/small-alarmone-synthetases-as-novel-bacterial-rna-binding-proteins
#10
Vasili Hauryliuk, Gemma C Atkinson
The alarmone nucleotides guanosine pentaphosphate (pppGpp) and tetraphosphate (ppGpp), collectively referred to as (p)ppGpp, are key regulators of bacterial growth, stress adaptation, antibiotic tolerance and pathogenicity. We have recently shown that the Small Alarmone Synthetase (SAS) RelQ from the Gram-positive pathogen Enterococcus faecalis has an RNA-binding activity (Beljantseva et al. 2017). RelQ's activities as an enzyme and as a RNA-binding protein are mutually incompatible: binding of single-stranded RNA potently inhibits (p)ppGpp synthesis in a sequence-specific manner, and RelQ's enzymatic activity destabilizes the RNA:RelQ complex...
August 18, 2017: RNA Biology
https://www.readbyqxmd.com/read/28820085/reclassification-of-mzabimyces-algeriensis-saker-et-al-2015-as-halopolyspora-algeriensis-comb-nov
#11
Hangxian Lai, Yingying Jiang, Rafika Saker, Xiu Chen, Noureddine Bouras, Hans-Peter Klenk, Xiaomin Wei, Yi Jiang, Nasserdine Sabaou
Halopolyspora alba AFM10251T was proposed to represent a novel species of a new genus belonging to the family Actinopolysporaceae in a previous study. The family Mzabimycetaceae, containing one genus, Mzabimyces, was proposed subsequently and Mzabimyces algeriensis H195T was the type strain. However, analysis of 16S rRNA gene sequence similarity showed that the two strains were highly similar (99.2 %). Phenotypic and chemotaxonomic data, as well as DNA-DNA hybridization confirmed that the two strains are different genomic species of the same genus, Halopolyspora...
August 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28819799/accuracy-of-cannulated-pedicle-screw-versus-conventional-pedicle-screw-for-extra-pedicular-screw-placement-in-dysplastic-pedicles-without-cancellous-channel-in-adolescent-idiopathic-scoliosis-a-computerized-tomography-ct-analysis
#12
Chee Kean Lee, Chris Yin Wei Chan, Siti Mariam Abd Gani, Mun Keong Kwan
PURPOSE: This study aimed at studying the accuracy and safety of extra-pedicular screw insertion for dysplastic pedicles in AIS comparing cannulated screw system versus conventional screw system. METHODS: 104 AIS patients with 1524 pedicle screws were evaluated using CT scan. 302 screws were inserted in dysplastic pedicles using fluoroscopic guidance technique. 155 screws were inserted using a cannulated system (Group 1), whereas 147 screws were inserted using standard screws (Group 2)...
August 17, 2017: European Spine Journal
https://www.readbyqxmd.com/read/28819779/curcuma-wenyujin-y-h-chen-et-c-ling-n-butyl-alcohol-extract-inhibits-ags-cell-helicobacter-pylori-caga-vaca-promoted-invasiveness-by-down-regulating-caudal-type-homeobox-transcription-factor-and-claudin-2-expression
#13
Hai-Feng Jin, Jin-Feng Dai, Li-Na Meng, Bin Lu
OBJECTIVE: To investigate the effects and possible mechanisms of action of Curcuma wenyujin Y. H. Chen et C. Ling n-Butyl alcohol extract (CWNAE) on repression of human gastric cancer (GC) AGS cell invasion induced by co-culturing with Helicobacter pylori (HP). METHODS: AGS cells were cultured with HP of positive or negative cytotoxin-associated gene A (CagA) and vacuolating cytotoxin gene A (VacA) expression (CagA+/- or VacA+/-) and divided into 5 group. Group A was cultured without HP as a control, Group B with HPCagA+VacA+, Group C with HPCagA-VacA-, Group D with HPCagA+VacA+ and CWNAE, and Group E with HPCagA-VacA- and CWNAE...
August 17, 2017: Chinese Journal of Integrative Medicine
https://www.readbyqxmd.com/read/28819776/tongxinluo-capsule-for-cardiac-syndrome-x-a-systematic-review-and-meta-analysis
#14
Hui-Min Mao, Mi Liu, Hua Qu, Li-Qiong Wang, Da-Zhuo Shi
OBJECTIVE: To evaluate the efficacy and safety of Tongxinluo Capsule (, TXL) for patients with cardiac syndrome X (CSX). METHODS: Randomized controlled trials (RCTs) regarding TXL in the treatment of CSX were searched in Chinese Biomedicine Literature Database, China National Knowledge Infrastructure, Chinese Scientific Journal Database, Wanfang Database, PubMed, EMBASE, Cochrane Central Register of Controlled Trial, websites of the Chinese and International Clinical Trial Registry platform up to June 30, 2015...
August 17, 2017: Chinese Journal of Integrative Medicine
https://www.readbyqxmd.com/read/28819754/development-of-the-hybrid-cells-in-series-model-to-simulate-ammonia-nutrient-pollutant-transport-along-the-umgeni-river
#15
Kayode O Olowe, Muthukrishnavellaisamy Kumarasamy
Discharge of organic waste results in high nutrient pollution of the water bodies which is a major menace to the environment. A high quantity of nutrients such as ammonia causes a reduction in the dissolved oxygen level and induces algal growth in the water bodies. Water quality models have been the tools to evaluate the rate at which streams can disperse the pollutants they receive. Many water quality models are flawed either because of their inadequacy to completely simulate the advection component of the pollutant transport, or because of the limited application of the models, due to inaccurate estimation of model parameters...
August 17, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28819742/authors-reply-to-mcmahon-et-al-comment-on-anthropometric-and-physical-qualities-of-elite-male-youth-rugby-league-players
#16
Kevin Till, Sean Scantlebury, Ben Jones
No abstract text is available yet for this article.
August 17, 2017: Sports Medicine
https://www.readbyqxmd.com/read/28819648/response-to-comment-on-how-will-induced-seismicity-in-oklahoma-respond-to-decreased-saltwater-injection-rates
#17
Cornelius Langenbruch, Mark D Zoback
Goebel et al. question our forecasted response of induced seismicity to reduction of saltwater injection rates in north-central Oklahoma and raise the concern that "the probability of future damaging earthquakes may be underestimated." We compare our prediction to earthquake data recorded in the 8 months after publication. Observed seismicity rates and magnitudes agree with the forecast of our model. Our use of a probabilistic model accounts for uncertainties and observed M ≥ 4.5 to date confirm the conservative nature of our prediction...
August 2017: Science Advances
https://www.readbyqxmd.com/read/28819527/-evaluation-of-the-compliance-rate-of-partograph-use-in-a-tunisian-maternity-ward-a-targeted-clinical-audit
#18
Latifa Merzougui, Nedia Marwen, Tarek Barhoumi, Afef Ben Ltaeifa, Hajer Hannachi, Roukaya Jaballah, Ridha Fatnassi
Partograph is an essential tool in routine delivery room practices. It is a written reflection of the quality of mother and child health management during delivery and in the immediate post-partum period. Our study aims to assess compliance rate of partograph use within our maternity ward and to propose areas for improvement of its quality. We conducted a retrospective clinical audit of 400 patients? medical records who gave birth on the labour ward at University of Kairouan from 1 January to 31 December 2014...
2017: Pan African Medical Journal
https://www.readbyqxmd.com/read/28819453/s100-spect-uncovers-cellular-and-molecular-events-of-pre-metastatic-niche-formation-and-following-organ-specific-cancer-metastasis
#19
EDITORIAL
Masakiyo Sakaguchi
Great progress has been made in in vivo imaging for cancer metastasis. Eisenblaetter et al. developed an innovative S100A8/A9-specific single photon emission computed tomography (SPECT) probe for imaging and succeeded in detecting the metastatic organ favored by the cancer before the cancer arrival. By utilizing this advanced method, researchers have also found that cancer-derived monocytes are the main source of the abundant production of S100A8/A9 in the pre-metastatic area. The CCL2-CCR2 axis is associated with S100A8/A9 production...
2017: Theranostics
https://www.readbyqxmd.com/read/28819297/energy-free-machine-learning-force-field-for-aluminum
#20
Ivan Kruglov, Oleg Sergeev, Alexey Yanilkin, Artem R Oganov
We used the machine learning technique of Li et al. (PRL 114, 2015) for molecular dynamics simulations. Atomic configurations were described by feature matrix based on internal vectors, and linear regression was used as a learning technique. We implemented this approach in the LAMMPS code. The method was applied to crystalline and liquid aluminum and uranium at different temperatures and densities, and showed the highest accuracy among different published potentials. Phonon density of states, entropy and melting temperature of aluminum were calculated using this machine learning potential...
August 17, 2017: Scientific Reports
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