Yih-Ping Su, Selena Y Lin, Ih-Jen Su, Yu-Lan Kao, Shih-Chun Shen, Joshua P Earl, Garth D Ehrlich, Cheng-Yi Chen, Wenya Huang, Ying-Hsiu Su, Hung-Wen Tsai
Ground glass hepatocytes (GGHs) have been associated with hepatocellular carcinoma (HCC) recurrence and poor prognosis. We previously demonstrated that pre-S expression in some GGHs is resistant to current hepatitis B virus (HBV) antiviral therapies. This study aimed to investigate whether integrated HBV DNA (iDNA) is the primary HBV DNA species responsible for sustained pre-S expression in GGH after effective antiviral therapy. We characterized 10 sets of micro-dissected, formalin-fixed-paraffin-embedded, and frozen GGH, HCC, and adjacent hepatitis B surface antigen-negative stained tissues for iDNA, pre-S deletions, and the quantity of covalently closed circular DNA...
January 2024: Journal of Medical Virology