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https://www.readbyqxmd.com/read/29220379/zebrafish-skeleton-development-high-resolution-micro-ct-and-fib-sem-block-surface-serial-imaging-for-phenotype-identification
#1
Jeremie Silvent, Anat Akiva, Vlad Brumfeld, Natalie Reznikov, Katya Rechav, Karina Yaniv, Lia Addadi, Steve Weiner
Although bone is one of the most studied living materials, many questions about the manner in which bones form remain unresolved, including fine details of the skeletal structure during development. In this study, we monitored skeleton development of zebrafish larvae, using calcein fluorescence, high-resolution micro-CT 3D images and FIB-SEM in the block surface serial imaging mode. We compared calcein staining of the skeletons of the wild type and nacre mutants, which are transparent zebrafish, with micro-CT for the first 30 days post fertilization embryos, and identified significant differences...
2017: PloS One
https://www.readbyqxmd.com/read/29220094/osteocytes-play-an-important-role-in-experimental-periodontitis-in-healthy-and-diabetic-mice-through-expression-of-rankl
#2
Dana T Graves, Ahmed Alshabab, Mayra Laino Albiero, Marcelo Mattos, Joice Dias Correa, Shanshan Chen, Yang Yang
AIM: Periodontitis results from bacteria induced inflammation. A key cytokine, RANKL is produced by a number of cell types. The cellular source of RANKL critical for periodontitis has not been established. METHODS: We induced periodontal bone loss by oral inoculation of Porphyromonas gingivalis and Fusobacterium nucleatum in both normoglycemic and streptozotocin induced type 1 diabetic mice. Experimental transgenic mice had osteocyte specific deletion of floxed RANKL mediated by DMP-1 driven Cre recombinase...
December 8, 2017: Journal of Clinical Periodontology
https://www.readbyqxmd.com/read/29220087/dual-energy-based-metal-segmentation-for-metal-artifact-reduction-in-dental-computed-tomography
#3
Mohamed A A Hegazy, Mohamed Elsayed Eldib, Daniel Hernandez, Myung Hye Cho, Min Hyoung Cho, Soo Yeol Lee
PURPOSE: In a dental CT scan, the presence of dental fillings or dental implants generates severe metal artifacts that often compromise readability of the CT images. Many metal artifact reduction (MAR) techniques have been introduced, but dental CT scans still suffer from severe metal artifacts particularly when multiple dental fillings or implants exist around the region of interest. The high attenuation coefficient of teeth often causes erroneous metal segmentation, compromising the MAR performance...
December 8, 2017: Medical Physics
https://www.readbyqxmd.com/read/29219117/prediction-of-radiation-necrosis-in-a-rodent-model-using-magnetic-resonance-imaging-apparent-transverse-relaxation-r-sub-2-sub-sup-sup
#4
Jean-Guy Belliveau, Michael D Jensen, James M P Stewart, Igor Solovey, Martyn L Klassen, Glenn S Bauman, Ravi S Menon
Radiation necrosis remains an irreversible long-term side-effect following radiotherapy to the brain. The ability to predict areas that could ultimately develop into necrosis could lead to prevention and management of radiation necrosis. Materials and Methods: Fischer 344 rats were irradiated using two platforms (micro-CT irradiator and x-RAD 225 IGRT) with radiation up to 30 Gy for the micro-CT and 40 Gy for the xRAD-224 to half the brain. Animals were subsequently imaged using a 9.4T MRI scanner every 2-4 weeks for up to 28 weeks using a 7-echo gradient echo sequence...
December 8, 2017: Physics in Medicine and Biology
https://www.readbyqxmd.com/read/29218776/is-the-consumption-of-snail-meat-actually-healthy-an-analysis-of-the-osteotropic-influence-of-snail-meat-as-a-sole-source-of-protein-in-growing-rats
#5
R P Radzki, M Bieńko, P Polak, K Szkucik, M Ziomek, M Ostapiuk, J Bieniaś
The study was aimed at determining the osteotropic effects of diets containing snail meat as a sole protein source. In our experiment, we tested three different diets incorporating snail meat originating from Helix pomatia (HP), Cornu.aspesa maxima (CAM) and Cornu.aspersum aspersum (CAA) and compared these to a control diet (CON) in which casein was the source of protein. In all diets, the protein content amounted to 10%, as calculated on a dry weight basis. In the study, forty male Wistar rats with an initial body mass of 50 ± 2 g were randomly placed within the control and three experimental groups...
December 7, 2017: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/29217091/microstructural-changes-associated-with-osteoporosis-negatively-affect-loading-induced-fluid-flow-around-osteocytes-in-cortical-bone
#6
Vittorio Gatti, Evan M Azoulay, Susannah P Fritton
Loading-induced interstitial fluid flow in the microporosities of bone is critical for osteocyte mechanotransduction and for the maintenance of tissue health, enhancing convective transport in the lacunar-canalicular system. In recent studies, our group has reported alterations of bone's vascular porosity and lacunar-canalicular system microarchitecture in a rat model of postmenopausal osteoporosis. In this work, poroelastic finite element analysis was used to investigate whether these microstructural changes can affect interstitial fluid flow around osteocytes...
November 16, 2017: Journal of Biomechanics
https://www.readbyqxmd.com/read/29216199/the-potential-of-statistical-shape-modelling-for-geometric-morphometric-analysis-of-human-teeth-in-archaeological-research
#7
Christopher Woods, Christianne Fernee, Martin Browne, Sonia Zakrzewski, Alexander Dickinson
This paper introduces statistical shape modelling (SSM) for use in osteoarchaeology research. SSM is a full field, multi-material analytical technique, and is presented as a supplementary geometric morphometric (GM) tool. Lower mandibular canines from two archaeological populations and one modern population were sampled, digitised using micro-CT, aligned, registered to a baseline and statistically modelled using principal component analysis (PCA). Sample material properties were incorporated as a binary enamel/dentin parameter...
2017: PloS One
https://www.readbyqxmd.com/read/29215685/micro-ct-evaluation-of-root-and-canal-morphology-of-mandibular-first-premolars-with-radicular-grooves
#8
Emanuele Boschetti, Yara Terezinha Correa Silva-Sousa, Jardel Francisco Mazzi-Chaves, Graziela Bianchi Leoni, Marco Aurélio Versiani, Jesus Djalma Pécora, Paulo Cesar Saquy, Manoel Damião de Sousa-Neto
The aim of this study was to evaluate morphological features of 70 single-rooted mandibular first premolars with radicular grooves (RG) using micro-CT technology. Teeth were scanned and evaluated regarding the morphology of the roots and root canals as well as length, depth and percentage frequency location of the RG. Volume, surface area and Structure Model Index (SMI) of the canals were measured for the full root length. Two-dimensional parameters and frequency of canal orifices were evaluated at 1, 2, and 3 mm levels from the apical foramen...
September 2017: Brazilian Dental Journal
https://www.readbyqxmd.com/read/29212295/inhibitory-effects-of-boesenbergia-pandurata-on-age-related-periodontal-inflammation-and-alveolar-bone-loss-in-fischer-344-rats
#9
Haebom Kim, Changhee Kim, Do Un Kim, Hee Chul Chung, Jae-Kwan Hwang
Periodontitis, an infective disease caused by oral pathogens and the intrinsic aging process, results in the destruction of periodontal tissues and alveolar bone loss. This study investigated whether Boesenbergia pandurata extract (BPE) standardized with panduratin A exerted anti-periodontitis effects using an aging model representative of naturally occurring periodontitis. In aged rats, the oral administration of BPE (200 mg/kg/day) for 8 weeks significantly reduced the mRNA and protein expression of interleukin (IL)-1β, nuclear factor-kappaB (NF-κB), matrix metalloproteinase (MMP)-2, and MMP-8 in gingival tissues (p < 0...
December 8, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29211127/evaluation-of-stress-distribution-in-endodontically-weakened-teeth-restored-with-different-crown-materials-3d-fea-analysis
#10
Izabela C M Moris, Carolina Alves Moscardini, Luana Kelle Batista Moura, Yara Teresinha Corrêa Silva-Sousa, Erica Alves Gomes
This study evaluated the stress distribution in endodontically treated teeth, weakened (W) or not weakened (NW), restored with different materials of prosthetic crown using 3D-FEA. Models of a maxillary canine were constructed based on micro-CT images and divided into the groups: G1 (control) - sound tooth; G2 to G7 - endodontically treated teeth restored with glass fiber post (GFP); which G2 to G4 simulated NW root and G5 to G7 simulated W root. For crown material the teeth were restored with: G2 and G5: metallic coping and ceramic veneering, G3 and G6: zirconia coping and ceramic veneering, G4 and G7: alumina coping and ceramic veneering...
November 2017: Brazilian Dental Journal
https://www.readbyqxmd.com/read/29211126/effect-of-reciprocating-systems-and-working-lengths-on-apical-microcrack-development-a-micro-ct-study
#11
Bruna Paloma de Oliveira, Andréa Cruz Câmara, Daniel Amancio Duarte, Richard John Heck, Antonio Celso Dantas Antonino, Carlos Menezes Aguiar
The objective of this study was to evaluate the effect of root canal preparation with single-file reciprocating systems at different working lengths on the development of apical microcracks using micro-computed tomographic (micro-CT) imaging. Forty extracted human mandibular incisors were randomly assigned to 4 groups (n=10) according to the systems and working length used to prepare the root canals: Group A - WaveOne Gold at apical foramen (AF), Group B - WaveOne Gold 1 mm short of the AF (AF-1 mm), Group C - Unicone (AF) and Group D - Unicone (AF-1 mm)...
November 2017: Brazilian Dental Journal
https://www.readbyqxmd.com/read/29210297/modern-morphological-methods-for-tadpole-studies-a-comparison-of-micro-ct-and-clearing-and-staining-protocols-modified-for-frog-larvae
#12
M Krings, H Müller, M J Heneka, D Rödder
Micro-computed tomography (micro-CT) scanning and three-dimensional reconstruction have revolutionized morphological studies. Whereas species descriptions and comparative studies formerly were based on external appearance and dissection, we now can visualize muscles, skeleton and viscera in intact animals. In most cases, visualization of internal structures depends on appropriate staining methods. Staining with iodine, phosphotungstic acid (PTA) and osmium tetroxide are established methods, but some problems remain...
December 6, 2017: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/29209409/contrast-enhanced-3d-micro-ct-of-plant-tissues-using-different-impregnation-techniques
#13
Zi Wang, Pieter Verboven, Bart Nicolai
Background: X-ray micro-CT has increasingly been used for 3D imaging of plant structures. At the micrometer resolution however, limitations in X-ray contrast often lead to datasets with poor qualitative and quantitative measures, especially within dense cell clusters of plant tissue specimens. The current study developed protocols for delivering a cesium based contrast enhancing solution to varying plant tissue specimens for the purpose of improving 3D tissue structure characterization within plant specimens, accompanied by new image processing workflows to extract the additional data generated by the contrast enhanced scans...
2017: Plant Methods
https://www.readbyqxmd.com/read/29208237/imaging-the-human-placental-microcirculation-with-micro-focus-computed-tomography-optimisation-of-tissue-preparation-and-image-acquisition
#14
Rosalind Pratt, J Ciaran Hutchinson, Andrew Melbourne, Maria A Zuluaga, Alex Virasami, Tom Vercauteren, Sebastien Ourselin, Neil J Sebire, Owen J Arthurs, Anna L David
Micro-CT provides 3D volume imaging with spatial resolution at the micrometre scale. We investigated the optimal human placenta tissue preparation (contrast agent, perfusion pressure, perfusion location and perfusion vessel) and imaging (energy, target material, exposure time and frames) parameters. Microfil (Flow Tech, Carver, MA) produced better fill than Barium sulphate (84.1%(±11.5%)vs70.4%(±18.02%) p = 0.01). Perfusion via umbilical artery produced better fill than via chorionic vessels (83.8%(±17...
December 2017: Placenta
https://www.readbyqxmd.com/read/29205681/meroistic-oogenesis-of-drosophila-in-section-in-situ
#15
Alexandra L Mattei, Mariana F Wolfner
Some insects, such as Drosophila melanogaster, produce eggs by meroistic oogenesis. In this process, the developing oocyte remains connected by cytoplasmic bridges to its sister "nurse" cells, which synthesize molecules and organelles for the future embryo. In the ovary, shown brighter in this micro-CT (X-ray computed tomography) scan of the abdomen of a female fly at 8 h post-mating, egg-chambers made of oocyte and nurse cells are lined up progressively in tubules called ovarioles.
December 4, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/29201897/combined-treatment-with-an-anticoagulant-and-a-vasodilator-prevents-steroid-associated-osteonecrosis-of-rabbit-femoral-heads-by-improving-hypercoagulability
#16
Fang Cao, Ge Liu, Wei Wang, Benjie Wang, Xiaowei Wei, Faqiang Lu, Fan Yang, Kai Kang, Yongxuan Wang, Jiahui Yang, Kairong Qin, Dewei Zhao
Steroid-associated osteonecrosis of the femoral head remains a challenging problem in orthopedics worldwide. One pathomechanism is ischemia of the femoral head, as a result of thrombus formation and vasoconstriction. The present study investigates the effects of combination prevention with enoxaparin and EGb 761 on steroid-associated ONFH in rabbits. Rabbits were randomly divided into 5 groups (control group, model group, enoxaparin group, ginkgo group, and combination group). With the exception of the control group, the groups of rabbits were modeled with lipopolysaccharide and methylprednisolone acetate...
2017: BioMed Research International
https://www.readbyqxmd.com/read/29198939/three-dimensional-computed-tomography-measurement-accuracy-of-varying-hill-sachs-lesion-size
#17
Anthony Ho, Michael D Kurdziel, Denise M Koueiter, J Michael Wiater
BACKGROUND: The glenoid track concept has been proposed to correlate shoulder stability with bone loss. Accurate assessment of Hill-Sachs lesion size preoperatively may affect surgical planning and postoperative outcomes; however, no measurement method has been universally accepted. This study aimed to assess the accuracy and reliability of measuring Hill-Sachs lesion sizes using 3-dimensional (3D) computed tomography (CT). METHODS: Nine polyurethane humerus bone substitutes were used to create Hill-Sachs lesions of varying sizes with a combination of lesion depth (shallow, intermediate, and deep) and width (small, medium, and large)...
November 30, 2017: Journal of Shoulder and Elbow Surgery
https://www.readbyqxmd.com/read/29198851/real-time-observations-of-tooth-demineralization-in-3-dimensions-using-x-ray-microtomography
#18
Graham Roy Davis, David Mills, Paul Anderson
OBJECTIVES: The immediate aim of this study was to develop and test a method to record, visualize and quantify real-time demineralization (or remineralization) of teeth in vitro using X-ray microtomography (XMT or micro-CT). The longer term objective is to improve understanding of demineralization processes, allowing the creation of better artificial models of dental caries and better quantification of the efficacy of caries treatment and prevention regimes. METHODS: For demineralization studies, the tooth is mounted in a plastic container through which the demineralizing solution is circulated during simultaneous repeated scanning...
November 30, 2017: Journal of Dentistry
https://www.readbyqxmd.com/read/29196647/injectable-nanohydroxyapatite-chitosan-gelatin-micro-scaffolds-induce-regeneration-of-knee-subchondral-bone-lesions
#19
B Wang, W Liu, D Xing, R Li, C Lv, Y Li, X Yan, Y Ke, Y Xu, Y Du, J Lin
Subchondral bone has been identified as an attractive target for KOA. To determine whether a minimally invasive micro-scaffolds could be used to induce regeneration of knee subchondral bone lesions, and to examine the protective effect of subchondral bone regeneration on upper cartilage, a ready-to-use injectable treatment with nanohydroxyapatite-chitosan-gelatin micro-scaffolds (HaCGMs) is proposed. Human-infrapatellar-fat-pad-derived adipose stem cells (IPFP-ASCs) were used as a cellular model to examine the osteo-inductivity and biocompatibility of HaCGMs, which were feasibly obtained with potency for multi-potential differentiations...
December 1, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29196345/mitoprotection-attenuates-myocardial-vascular-impairment-in-porcine-metabolic-syndrome
#20
Fang Yuan, Ahmad F Hedayat, Christopher M Ferguson, Amir Lerman, Lilach O Lerman, Alfonso Eirin
INTRODUCTION: The metabolic syndrome (MetS) leads to cardiac vascular injury, which may reflect in increased retention of endothelial progenitor cells (EPC). Coronary endothelial cell (EC) mitochondria partly regulate vascular function and structure. We hypothesized that chronic mitoprotection would preserve EC mitochondria and attenuate coronary vascular injury and dysfunction in swine MetS. METHODS: Pigs were studied after 16 weeks of diet-induced MetS, MetS treated for the last 4 weeks with the mitochondria-targeted peptide elamipretide (ELAM, 0...
December 1, 2017: American Journal of Physiology. Heart and Circulatory Physiology
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