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Liquid Handling

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https://www.readbyqxmd.com/read/28095176/a-flexible-workflow-for-automated-bioluminescent-kinase-selectivity-profiling
#1
Tracy Worzella, Matt Butzler, Jacquelyn Hennek, Seth Hanson, Laura Simdon, Said Goueli, Cris Cowan, Hicham Zegzouti
Kinase profiling during drug discovery is a necessary process to confirm inhibitor selectivity and assess off-target activities. However, cost and logistical limitations prevent profiling activities from being performed in-house. We describe the development of an automated and flexible kinase profiling workflow that combines ready-to-use kinase enzymes and substrates in convenient eight-tube strips, a bench-top liquid handling device, ADP-Glo Kinase Assay (Promega, Madison, WI) technology to quantify enzyme activity, and a multimode detection instrument...
November 1, 2016: Journal of Laboratory Automation
https://www.readbyqxmd.com/read/28093629/liquid-release-as-a-source-of-potential-drug-exposure-during-the-handling-of-intravenous-infusions-in-nursing
#2
Verena Segner, Renate Kimbel, Philipp Jochems, André Heinemann, Stephan Letzel, Daniel Wollschläger, Bernd Roßbach
PURPOSE: This study aims at experimentally determining the incidence and extent of liquid releases onto the operator's hands and into the work environment during common nursing operations involving infusions. METHODS: A sequence of operations related to the preparation and administration of infusions was conducted by three subjects for 15 times each using fluorescein marked infusion solutions and two different infusion sets (standard set vs. safety-optimized set)...
January 16, 2017: International Archives of Occupational and Environmental Health
https://www.readbyqxmd.com/read/28078439/liquid-based-versus-conventional-cytology-of-oral-brush-biopsies-a-split-sample-pilot-study
#3
T W Remmerbach, N Pomjanski, U Bauer, H Neumann
OBJECTIVE: The aim of this prospective split sample study was to evaluate the applicability of liquid-based cytology (LBC) of oral brush biopsies for detection of oral cancer. METHODS: Two different preparation techniques were investigated: the conventional transfer procedure to glass slides and the LBC preparation method. The obtainments of epithelial cells were performed five times with a nylon brush and transferred onto five glass slides. Additionally, the brushes, which were normally discarded, were stored in a fixative solution...
January 11, 2017: Clinical Oral Investigations
https://www.readbyqxmd.com/read/28074108/cellulolytic-robolector-towards-an-automated-high-throughput-screening-platform-for-recombinant-cellulase-expression
#4
Martina Mühlmann, Martin Kunze, Joaquim Ribeiro, Bertram Geinitz, Christian Lehmann, Ulrich Schwaneberg, Ulrich Commandeur, Jochen Büchs
BACKGROUND: Cellulases are key player in the hydrolyzation of cellulose. Unfortunately, this reaction is slow and a bottleneck in the process chain from biomass to intermediates and biofuels due to low activities of the enzymes. To overcome this draw back, a lot of effort is put into the area of protein engineering, to modify these enzymes by directed evolution or rational design. Huge clone libraries are constructed and have to be screened for improved variants. High-throughput screening is the method of choice to tackle this experimental effort, but up to now only a few process steps are adapted to automated platforms and little attention has been turned to the reproducibility of clone rankings...
2017: Journal of Biological Engineering
https://www.readbyqxmd.com/read/28054367/development-of-an-uplc-ms-ms-method-for-routine-therapeutic-drug-monitoring-of-aripiprazole-amisulpride-olanzapine-paliperidone-and-ziprasidone-with-a-discussion-of-their-therapeutic-reference-ranges-for-chinese-patients
#5
Shao-Ting Wang, Yan Li
A highly feasible and reliable ultra-high performance liquid chromatography tandem mass spectrometry method was presented for therapeutic drug monitoring of five anti-schizophrenic drugs (amisulpride, olanzapine, aripiprazole, paliperidone and ziprasidone) simultaneously. To meet the requirements of practical clinical usage (easy-handling, high-throughput and cost effective), the pretreatment process has been especially simplified (only including protein precipitation and mobile phase dilution steps) and the UPLC separation cycle was set within 6 minutes...
January 5, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28044289/digital-microfluidics-assisted-sealing-of-individual-magnetic-particles-in-femtoliter-sized-reaction-wells-for-single-molecule-detection
#6
Deborah Decrop, Elena Pérez Ruiz, Phalguni Tewari Kumar, Lisa Tripodi, Tadej Kokalj, Jeroen Lammertyn
Digital microfluidics has emerged in the last years as a promising liquid handling technology for a variety of applications. Here, we describe in detail how to build up an electrowetting-on-dielectric-based digital microfluidic chip with unique advantages for performing single-molecule detection. We illustrate how superparamagnetic particles can be printed with very high loading efficiency (over 98 %) and single-particle resolution in the microwell array patterned in the Teflon-AF(®) surface of the grounding plate of the chip...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28044285/capillary-driven-microfluidic-chips-for-miniaturized-immunoassays-patterning-capture-antibodies-using-microcontact-printing-and-dry-film-resists
#7
Yuksel Temiz, Robert D Lovchik, Emmanuel Delamarche
The miniaturization of immunoassays using microfluidic devices is attractive for many applications, but an important challenge remains the patterning of capture antibodies (cAbs) on the surface of microfluidic structures. Here, we describe how to pattern cAbs on planar poly(dimethylsiloxane) (PDMS) stamps and how to microcontact print the cAbs on a dry-film resist (DFR). DFRs are new types of photoresists having excellent chemical resistance and good mechanical, adhesive, and optical properties. Instead of being liquid photoresists, DFRs are thin layers that are easy to handle, cut, photo-pattern, and laminate over surfaces...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28034712/evaluation-of-a-digital-dispenser-for-direct-curve-dilutions-in-a-vaccine-potency-assay
#8
Christopher Roselle, Dana Whitehouse, Thy Follmer, Fran Ansbro, Silikhone Bouaraphan, Liming Guan, Sha-Ke Wang, Mary Shank-Retzlaff, Thorsten Verch
Dilutions are a common source of analytical error, both in terms of accuracy and precision, and a common source of analyst mistakes. When serial dilutions are used, errors compound, even when employing laboratory automation. Direct point dilutions instead of serial dilutions can reduce error but is often impractical as they require either large diluent volumes or very small sample volumes when performed with traditional liquid handling equipment. We evaluated preparation of dilution curves using a picoliter digital dispenser, the HP, Inc...
December 27, 2016: Journal of Immunological Methods
https://www.readbyqxmd.com/read/28029545/highly-sensitive-lc-ms-ms-methods-for-urinary-biological-monitoring-of-occupational-exposure-to-cyclophosphamide-ifosfamide-and-methotrexate-antineoplastic-drugs-and-routine-application
#9
Mireille Canal-Raffin, Karim Khennoufa, Béatrice Martinez, Yves Goujon, Celia Folch, Dominique Ducint, Karine Titier, Patrick Brochard, Catherine Verdun-Esquer, Mathieu Molimard
Highly sensitive ESI-LC-MS/MS methods were developed for urinary biological monitoring of occupational exposure to cyclophosphamide (CP), ifosfamide (IF), and methotrexate (MTX), which are hazardous antineoplastic drugs frequently handled by healthcare professionals. Extraction methods consisted of liquid/liquid extraction for simultaneous urinary CP and IF assays, and of solid phase extraction for the urinary MTX assay. A good linearity (r2>0.997), precision (CV<14.6%), and accuracy (bias<9.9%) were achieved for all compounds...
October 21, 2016: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28028593/quantification-of-dimethylsulfoniopropionate-dmsp-in-acropora-spp-of-reef-building-coral-using-mass-spectrometry-with-deuterated-internal-standard
#10
Hilton B Swan, Elisabeth S M Deschaseaux, Graham B Jones, Bradley D Eyre
Dimethylsulfoniopropionate (DMSP) in scleractinian coral is usually analysed indirectly as dimethylsulfide (DMS) using gas chromatography (GC) with a sulfur-specific detector. We developed a headspace GC method for mass spectral analysis of DMSP in branching coral where hexa-deuterated DMSP (d 6 -DMSP) was added to samples and standards to optimise the analytical precision and quantitative accuracy. Using this indirect HS-GC-MS method, we show that common coral sample handling techniques did not alter DMSP concentrations in Acropora aspera and that endogenous DMS was insignificant compared to the store of DMSP in A...
December 27, 2016: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28028591/determination-of-diuron-terbuthylazine-and-terbutryn-in-wastewater-and-soil-by-micellar-liquid-chromatography
#11
Susana Pitarch-Andrés, Pasqual Roca-Genovés, Juan Peris-Vicente, Josep Esteve-Romero
An analytical method for the quantification of the herbicides and algaecides diuron, terbuthylazine, and terbutryn in wastewater and soil by micellar liquid chromatography was developed. The sample preparation was expedited to reduce the number of intermediate steps and the use of chemicals. The analytes in soils were recovered by ultrasonication in the mobile phase. The obtained supernatant and the water samples were directly injected, thus avoiding intermediate steps. The chromatographic behavior of the analytes, depending on the surfactant and alcohol was studied, in order to optimize the chromatographic run, by a chemometrical approach...
December 27, 2016: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28027450/enabling-1536-well-high-throughput-cell-based-screening-through-the-application-of-novel-centrifugal-plate-washing
#12
Sinead Knight, Helen Plant, Lisa McWilliams, David Murray, Rebecca Dixon-Steele, Anet Varghese, Paul Harper, Anna Ramne, Paula McArdle, Susanna Engberg, Neil Bennett, Carolyn Blackett, Mark Wigglesworth
Cell-based assays have long been important within hit discovery paradigms; however, improving the disease relevance of the assay system can positively affect the translation of small-molecule drug discovery, especially if adopted in the initial hit identification assay. Consequently, there is an increasing need for disease-relevant assay systems capable of running at large scale, including the use of induced pluripotent stem cells and donor-derived primary cells. Major hurdles to adopting these assays for high-throughput screening are the cost, availability of cells, and complex protocols...
December 1, 2016: Journal of Biomolecular Screening
https://www.readbyqxmd.com/read/28026934/modifying-mechanical-optical-properties-and-thermal-processability-of-iridescent-cellulose-nanocrystal-films-using-ionic-liquid
#13
Ping Liu, Xin Guo, Fuchun Nan, Yongxin Duan, Jianming Zhang
Iridescent films formed from the self-assembly of cellulose nanocrystals (CNCs) are brittle and difficult to handle or integrate within an industrial process. Here we present a simple approach to prepare iridescent CNC films with tunable pliability and coloration through the addition of ionic liquids (ILs) of 1-allyl-3-methylimidazolium chloride (AmimCl) as plasticizer. By using the un-dried CNC film as filter membrane and ILs solution as leaching liquor, it was found that the filtration process made ILs uniformly interpenetrate into CNC film due to the strong ionic interaction between CNC and AmimCl...
December 27, 2016: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28025509/a-high-throughput-rna-extraction-for-sprouted-single-seed-barley-hordeum-vulgare-l-rich-in-polysaccharides
#14
Abdur Rashid, Thomas Baldwin, Michael Gines, Phil Bregitzer, Kathy Esvelt Klos
Germinated seed from cereal crops including barley (Hordeum vulgare L.) is an important tissue to extract RNA and analyze expression levels of genes that control aspects of germination. These tissues are rich in polysaccharides and most methods for RNA extraction are not suitable to handle the excess polysaccharides. Here, we compare the current methods for RNA extraction applicable to germinated barley tissue. We found that although some of these standard methods produced high-quality RNA, the process of extraction was drastically slow, mostly because the frozen seed tissue powder from liquid N₂ grinding became recalcitrant to buffer mixing...
December 22, 2016: Plants (Basel, Switzerland)
https://www.readbyqxmd.com/read/28012307/a-sensitive-lc-ms-ms-method-for-analysis-of-pericyazine-in-presence-of-7-hydroxypericyazine-and-pericyazine-sulphoxide-in-human-plasma-and-its-application-to-a-comparative-bioequivalence-study-in-chinese-healthy-volunteers
#15
Hua Lin Cai, Yang Deng, Ping Fei Fang, SiSi Cao, Zhen Yan Hou, Yan Qin Wu, Xue Jiao Chen, Miao Yan, BiKui Zhang
A robust and highly sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for the determination of pericyazine in human plasma. The plasma sample was alkalized with sodium hydroxide solution and handled by liquid-liquid extraction with ethyl acetate after adding perphenazine as an internal standard (IS). The analytes were separated on an Ultimate™ AQ-C18 analytical column at 40°C, with a gradient elution consisting of A (aqueous phase: 5mM ammonium acetate buffer solution containing 0...
December 15, 2016: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28007184/preliminary-studies-on-the-stability-of-arsenolipids-implications-for-sample-handling-and-analysis
#16
Muslim Khan, Kevin A Francesconi
Human health risk assessments concerning arsenic are now estimating exposure through food in addition to exposure through drinking water. Intrinsic to this assessment is sample handling and preparation that maintains the arsenic species in the form that they occur in foods. We investigated the stability of three arsenolipids (two arsenic fatty acids, AsFA-362 and AsFA-388, and one arsenic hydrocarbon AsHC-332), common constituents of fish and algae, relevant to sample storage and transport, and to their preparation for quantitative measurements...
November 2016: Journal of Environmental Sciences (China)
https://www.readbyqxmd.com/read/28006013/procarcinogens-determination-and-evaluation-by-yeast-based-biosensor-transformed-with-plasmids-incorporating-rad54-reporter-construct-and-cytochrome-p450-genes
#17
Van Ngoc Bui, Thi Thu Huyen Nguyen, Chi Thanh Mai, Yvan Bettarel, Thi Yen Hoang, Thi Thuy Linh Trinh, Nam Hai Truong, Hoang Ha Chu, Vu Thanh Thanh Nguyen, Huu Duc Nguyen, Stefan Wölfl
In Vietnam, a great number of toxic substances, including carcinogens and procarcinogens, from industrial and agricultural activities, food production, and healthcare services are daily released into the environment. In the present study, we report the development of novel yeast-based biosensor systems to determine both genotoxic carcinogens and procarcinogens by cotransformation with two plasmids. One plasmid is carrying human CPR and CYP (CYP3A4, CYP2B6, or CYP2D6) genes, while the other contains the RAD54-GFP reporter construct...
2016: PloS One
https://www.readbyqxmd.com/read/28004916/liquid-organic-hydrogen-carriers-lohcs-toward-a-hydrogen-free-hydrogen-economy
#18
Patrick Preuster, Christian Papp, Peter Wasserscheid
The need to drastically reduce CO2 emissions will lead to the transformation of our current, carbon-based energy system to a more sustainable, renewable-based one. In this process, hydrogen will gain increasing importance as secondary energy vector. Energy storage requirements on the TWh scale (to bridge extended times of low wind and sun harvest) and global logistics of renewable energy equivalents will create additional driving forces toward a future hydrogen economy. However, the nature of hydrogen requires dedicated infrastructures, and this has prevented so far the introduction of elemental hydrogen into the energy sector to a large extent...
December 22, 2016: Accounts of Chemical Research
https://www.readbyqxmd.com/read/28004172/hydrophobic-ionic-liquids-for-quantitative-bacterial-cell-lysis-with-subsequent-dna-quantification
#19
Sabine Fuchs-Telka, Susanne Fister, Patrick-Julian Mester, Martin Wagner, Peter Rossmanith
DNA is one of the most frequently analyzed molecules in the life sciences. In this article we describe a simple and fast protocol for quantitative DNA isolation from bacteria based on hydrophobic ionic liquid supported cell lysis at elevated temperatures (120-150 °C) for subsequent PCR-based analysis. From a set of five hydrophobic ionic liquids, 1-butyl-1-methylpyrrolidinium bis(trifluoromethylsulfonyl)imide was identified as the most suitable for quantitative cell lysis and DNA extraction because of limited quantitative PCR inhibition by the aqueous eluate as well as no detectable DNA uptake...
December 21, 2016: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28003040/nutritional-and-microbiological-quality-of-commercial-and-homemade-blenderized-whole-food-enteral-diets-for-home-based-enteral-nutritional-therapy-in-adults
#20
Maricy Machado Cavalca Vieira, Valdirene Francisca Neves Santos, Andrea Bottoni, Tania Beninga Morais
BACKGROUND & AIMS: Serious nutritional and contamination risks may be involved in the preparation of blenderized tube-feeding diets and in the handling of commercial diets. Their nutritional and microbiological quality in home settings is unknown. The objective of this study was to assess the nutritional and microbiological quality of commercial enteral and homemade blenderized whole foods diets intended to adult patients in home nutritional therapy. METHODS: In a cross sectional study, 66 samples of commercial (CD) and noncommercial (NCD) enteral diets were collected at the homes of patients in home enteral nutritional therapy, 33 of each type...
December 1, 2016: Clinical Nutrition: Official Journal of the European Society of Parenteral and Enteral Nutrition
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