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Liquid Handling

Tomasz Szewczyk, Przemyslaw Janczak, Natalia Jezierska, Piotr Jurałowicz
BACKGROUND: Laparoscopic adjustable gastric banding is a well-established method of morbid obesity treatment. One of the complications is slippage. AIM: The aim of the study was to assess the prevalence, causes, and handling of slippage after LAGB in own material. MATERIAL AND METHODS: There were 740 procedures performed by one surgical team. The patients were selected according to the IFSO principles. The pars flaccida technique was applied and the position of the band was controlled...
April 24, 2017: Obesity Surgery
José A López-López, Esther Ogalla-Chozas, Pablo A Lara-Martín, Marina G Pintado-Herrera
Measuring the impact of PAHs in seawater samples is often difficult due to the low concentrations in which they appear and the complexity of the sample matrix. Traditional methods for sample preparation such as liquid-liquid extraction and solid phase extraction require the use of excessive amounts of solvents and reagents, and sample handling. In this work, hollow fiber liquid phase micro-extraction (HFLPME), in the configuration of solvent bar micro-extraction (SBME), was proposed as an environmentally friendly and more effective tool, for the extraction of the 16 priority PAHs from seawater samples...
April 21, 2017: Science of the Total Environment
Yanran Cao, Ann-Kristin Tveten, Anne Stene
Fish welfare is an important issue for growth of the aquaculture industry. Stress responses represent animal's natural reactions to challenging conditions and could be used as a welfare indicator. Cortisol level is relevant to fish welfare condition, and is a readily measured component of the primary stress response system. Generally, cortisol is measured by blood sampling. However, fish blood cortisol level could be instantly influenced by handling-stress at sampling. Fecal corticoid metabolites (FCM) are a mixture of several different metabolites with a wide range of polarities...
April 19, 2017: Fish & Shellfish Immunology
Gian Luca Salvagno, Elisa Danese, Giuseppe Lippi
The use of liquid chromatography-mass spectrometry (LC-MS) for both diagnostics and research purposes is rapidly growing in clinical laboratories. As for more conventional areas of in vitro diagnostic testing, many preanalytical variables have an impact on these techniques and may hence jeopardize the quality of tests results. The leading preanalytical variables include patient preparation, the nature of the blood collection tubes and additives, interference from spurious hemolysis, sample handling and management, composition of blood tubes, contamination, as well as storage conditions...
April 19, 2017: Clinical Biochemistry
Y Zhao, G Czilwik, V Klein, K Mitsakakis, R Zengerle, N Paust
We present a fully automated centrifugal microfluidic method for particle based protein immunoassays. Stick-pack technology is employed for pre-storage and release of liquid reagents. Quantitative layout of centrifugo-pneumatic particle handling, including timed valving, switching and pumping is assisted by network simulations. The automation is exclusively controlled by the spinning frequency and does not require any additional means. New centrifugal microfluidic process chains are developed in order to sequentially supply wash buffer based on frequency dependent stick-pack opening and pneumatic pumping to perform two washing steps from one stored wash buffer; pre-store and re-suspend functionalized microparticles on a disk; and switch between the path of the waste fluid and the path of the substrate reaction product with 100% efficiency...
April 20, 2017: Lab on a Chip
Lawrence S Engel, Richard K Kwok, Aubrey K Miller, Aaron Blair, Matthew D Curry, John A McGrath, Dale P Sandler, Polly Armsby, Susan Baker, Richard D Cohn, Edward E Gaunt, Audra Hodges, David Johndrow, Steven K Ramsey, Mark Stenzel, Patricia Stewart
The 2010 Deepwater Horizon (DWH) explosion in the Gulf of Mexico led to the largest ever marine oil spill by volume. The GuLF STUDY is investigating possible adverse human health effects associated with oil spill activities. One objective of the study was to utilize biological specimens from study participants to examine spill-related adverse health effects. This study describes the methods for collecting, processing, shipping, and storing specimens during the enrollment phase of the study. GuLF STUDY participants living in Gulf States (Alabama, Florida, Louisiana, Mississippi, and eastern Texas) were eligible to complete a home visit at enrollment, one to three years after the DWH explosion...
April 18, 2017: Journal of Toxicology and Environmental Health. Part A
Michael D Dickey
The use of liquid metals based on gallium for soft and stretchable electronics is discussed. This emerging class of electronics is motivated, in part, by the new opportunities that arise from devices that have mechanical properties similar to those encountered in the human experience, such as skin, tissue, textiles, and clothing. These types of electronics (e.g., wearable or implantable electronics, sensors for soft robotics, e-skin) must operate during deformation. Liquid metals are compelling materials for these applications because, in principle, they are infinitely deformable while retaining metallic conductivity...
April 18, 2017: Advanced Materials
Xue Sun, Xiao-Bing Cui, Hong-Mei Wen, Chen-Xiao Shan, Xin-Zhi Wang, An Kang, Chuan Chai, Wei Li
In the present study, the chemical compositions of Atractylodes macrocephala Koidz. (AMK) were analyzed systematically and influence of sulfur fumigation on the chemical profiles was evaluated by ultrafast flow liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry (UFLC-QTOF-MS) combined with multivariate statistical analysis. 52 components were detected from non-fumigated AMK (NF-AMK) and 28 components were newly produced after sulfur fumigation, out of which 59 major peaks were identified...
March 7, 2017: Journal of Pharmaceutical and Biomedical Analysis
Mariana Ruesgas-Ramon, Maria Cruz Figueroa-Espinoza, Erwann Durand
The green chemistry era has pushed the scientific community to investigate and implement new solvents in the phenolic compounds (PC) extraction, as alternative to the organic solvents which are toxic and may be dangerous. Recently, Deep Eutectic Solvents (DES) have been applied as extraction solvents of PC. They have the advantages to be biodegradable, easy to handle with very low toxicity. Nevertheless, the extraction process is affected by several factors: affinity between DES and the target compounds, the water content, the molar ratio between DES' starting molecules, the liquid:solid ratio between the DES and sample, and the conditions and extraction method...
April 17, 2017: Journal of Agricultural and Food Chemistry
Milla Heinälä, Katriina Ylinen, Tapani Tuomi, Tiina Santonen, Simo P Porras
The aim of the study was to assess occupational exposure to bisphenol A in Finland. Five companies took part in the research project: two paint factories (liquid and powder paints), a composite product factory, a thermal paper factory, and a tractor factory. Exposure was assessed by measuring total bisphenol A excretion (free and conjugated) from urine samples, and its concentrations in the air. The results revealed the specific work tasks in two of five companies in which significant occupational exposure to bisphenol A may occur...
January 1, 2017: Annals of Work Exposures and Health
Hojin Kim, In Ho Choi, Sanghyun Lee, Dong-Joon Won, Yong Suk Oh, Donghoon Kwon, Hyung Jin Sung, Sangmin Jeon, Joonwon Kim
This paper presents a deterministic bead-in-droplet ejection (BIDE) technique that regulates the precise distribution of microbeads in an ejected droplet. The deterministic BIDE was realized through the effective integration of a microfluidic single-particle handling technique with a liquid dispensing system. The integrated bead dispenser facilitates the transfer of the desired number of beads into a dispensing volume and the on-demand ejection of bead-encapsulated droplets. Single bead-encapsulated droplets were ejected every 3 s without any failure...
April 10, 2017: Scientific Reports
G J Hausammann, C L Bellac, M Sänger, M Gilgen
The main objective of this study was the standardization of the direct anti-A, anti-B haemagglutination assay for immunoglobulin products in microtitre plates and gelcards by automation on a liquid handling robot. In addition, the evaluation of the pipetted microtitre plates with a computer-controlled camera was investigated and these results were related to titres from visual live reading. The titres obtained with the automated and the manual assay in microtitre plates and gelcards were compared. They were in excellent agreement: the titres of samples processed with the automated method varied maximally one titre step relative to the median titres of the same sample analysed with the manual method...
April 4, 2017: Biologicals: Journal of the International Association of Biological Standardization
Stefan Altaner, Jonathan Puddick, Susanna A Wood, Daniel R Dietrich
Cyanobacteria can produce heptapetides called microcystins (MC) which are harmful to humans due to their ability to inhibit cellular protein phosphatases. Quantitation of these toxins can be hampered by their adsorption to common laboratory-ware during sample processing and analysis. Because of their structural diversity (>100 congeners) and different physico-chemical properties, they vary in their adsorption to surfaces. In this study, the adsorption of ten different MC congeners (encompassing non-arginated to doubly-arginated congeners) to common laboratory-ware was assessed using different solvent combinations...
April 6, 2017: Toxins
Hanan M Mukhaiber
Large quantities of hazardous medical waste are deposited into hospitals wastewaters and these substances could negatively impact public health if not handled properly. The authorities in the Syrian Arab Republic have realized the seriousness of the problem and have issued a number of laws, guidelines and standard specifications relating to this issue since 2004. This study offers a detailed review of these documents and compares them with related World Health Organization publications. A questionnaire was designed based on the national guide for safe management of healthcare waste in order to investigate the reality of liquid medical waste management in a sample of hospitals in Damascus...
March 30, 2017: Eastern Mediterranean Health Journal, la Revue de Santé de la Méditerranée Orientale
Jesús A Arellano, Taylor A Howell, James Gammon, Sungpil Cho, Margit M Janát-Amsbury, Bruce Gale
A high-throughput, microfluidic flow cell array (MFCA) system has been modified to enable drug screening against small-volume cell-, and tissue cultures. The MFCA is composed of a 3D channel network that simultaneously flows fluids through forty-eight 830 μm by 500 μm flow cells, which physically divide and fluidically seal an existing culture into multiple compartments when docked onto the surface of a cell or tissue culture dish. The modified system provides temperature (37 °C) and CO2/pH level controls, while continuously flowing solutions (media or other liquid such as drug suspensions) over the cells/tissues...
June 2017: Biomedical Microdevices
Xin Tang, Pingan Zhu, Ye Tian, Xuechang Zhou, Tiantian Kong, Liqiu Wang
The effective transfer of tiny liquid droplets is vital for a number of processes such as chemical and biological microassays. Inspired by the tarsi of meniscus-climbing insects, which can climb menisci by deforming the water/air interface, we developed a mechano-regulated surface consisting of a background mesh and a movable microfibre array with contrastive wettability. The adhesion of this mechano-regulated surface to liquid droplets can be reversibly switched through mechanical reconfiguration of the microfibre array...
April 4, 2017: Nature Communications
Henry Lin, Robert Woodrow Leighty, Scott Godfrey, Samantha Boran Wang
Perfusion medium was successfully developed based on our fed-batch platform basal and feed media. A systematic development approach was undertaken by first optimizing the ratios of fed-batch basal and feed media followed by targeted removal of unnecessary and redundant components. With this reduction in components, the medium could then be further concentrated by 2× to increase medium depth. The medium osmolality was also optimized where we found ∼360 mOsm/kg was desirable resulting in a residual culture osmolality of ∼300 mOsm/kg for our cell lines...
April 3, 2017: Biotechnology Progress
Victoria J Valerón Bergh, Elin Johannessen, Therese Andersen, Hanne Hjorth Tønnesen
Dry alginate foams intended for antibacterial photodynamic therapy (aPDT) of infected wounds were prepared. aPDT is a treatment modality involving light of appropriate wavelength, oxygen and a photosensitizer (PS) to produce radicals that attack biological targets (e.g. bacteria). The hydrophobic 5, 10, 15, 20-tetrakis(4-hydroxyphenyl)porphyrin (THPP) was chosen as a model PS and loaded into the foams. The foams contained a poloxamer (Pluronic F127) and/or β-cyclodextrin (βCD)-derivatives, i.e. methylβCD (MβCD), heptakis(2,6-di-O-methyl)-βCD (HkβCD) or hydroxypropylβCD (HPβCD)...
March 31, 2017: Pharmaceutical Development and Technology
Hanna E Landberg, Anna Axmon, Håkan Westberg, Håkan Tinnerberg
The use of exposure modelling tools for estimating chemical airborne exposure has increased since the European Union's REACH legislation for safe use of industrial chemicals came into force. Two tools that European Chemicals Agency recommends are Stoffenmanager® and the Advanced REACH Tool (ART). The aim of this study was to investigate the validity of these two exposure modelling tools by comparing the lack of agreement between estimated and measured exposure. We examined the airborne chemical exposure at companies in seven different types of industries: wood, printing, foundry, spray painting, flour milling, chemical industry, and plastic moulding industry...
March 11, 2017: Annals of Work Exposures and Health
David M DeJoy, Todd D Smith, Henok Woldu, Mari-Amanda Dyal, Andrea L Steege, James M Boiano
PURPOSE: Antineoplastic drugs pose risks to the healthcare workers who handle them. This fact notwithstanding, adherence to safe handing guidelines remains inconsistent and often poor. This study examined the effects of pertinent organizational safety practices and perceived safety climate on the use of personal protective equipment, engineering controls, and adverse events (spill/leak or skin contact) involving liquid antineoplastic drugs Method: Data for this study came from the 2011 National Institute for Occupational Safety and Health (NIOSH) Health and Safety Practices Survey of Healthcare Workers which included a sample of approximately 1,800 nurses who had administered liquid antineoplastic drugs during the past seven days...
March 22, 2017: Journal of Occupational and Environmental Hygiene
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