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Liquid Handling

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https://www.readbyqxmd.com/read/28213251/on-line-monitoring-of-in-vitro-oral-bioaccessibility-tests-as-front-end-to-liquid-chromatography-for-determination-of-chlorogenic-acid-isomers-in-dietary-supplements
#1
Daniel Kremr, David J Cocovi-Solberg, Petra Bajerová, Karel Ventura, Manuel Miró
A novel fully automated in-vitro oral dissolution test assay as a front-end to liquid chromatography has been developed and validated for on-line chemical profiling and monitoring of temporal release profiles of three caffeoylquinic acid (CQA) isomers, namely, 3-CQA,4-CQA and 5-CQA, known as chlorogenic acids, in dietary supplements. Tangential-flow filtration is harnessed as a sample processing approach for on-line handling of CQA containing extracts of hard gelatin capsules and introduction of protein-free samples into the liquid chromatograph...
May 1, 2017: Talanta
https://www.readbyqxmd.com/read/28207828/the-wide-spectrum-high-biocidal-potency-of-bioxy-formulation-when-dissolved-in-water-at-different-concentrations
#2
Dori Dagher, Ken Ungar, Richard Robison, Fadi Dagher
Traditional surface disinfectants that have long been applied in medicine, animal husbandry, manufacturing and institutions are inconvenient at best and dangerous at worst. Moreover, some of these substances have adverse environmental impacts: for example, quaternary ammonium compounds ("quats") are reproductive toxicants in both fish and mammals. Halogens are corrosive both to metals and living tissues, are highly reactive, can be readily neutralized by metals, and react with organic matter to form toxic, persistent by-products such as dioxins and furans...
2017: PloS One
https://www.readbyqxmd.com/read/28190338/towards-streamlined-identification-of-dioxin-like-compounds-in-environmental-samples-through-integration-of-suspension-bioassay
#3
Hongxia Xiao, Markus Brinkmann, Beat Thalmann, Andreas Schiwy, Sigrid Große Brinkhaus, Christine Achten, Kathrin Eichbaum, Carolin Gembé, Thomas Benjamin Seiler, Henner Hollert
Effect-directed analysis (EDA) is a powerful strategy to identify biologically active compounds in environmental samples. However, in current EDA studies, fractionation and handling procedures are laborious, consist of multiple evaporation steps, and thus bear the risk of contamination and decreased recoveries of the target compounds. The low resulting throughput has been one of the major bottlenecks of EDA. Here, we propose a high-throughput EDA (HT-EDA) work-flow combining reversed phase high-performance liquid chromatography (HPLC) fractionation of samples into 96-well microplates, followed by toxicity assessment in the micro-EROD bioassay with the wild-type rat hepatoma H4IIE cells, and chemical analysis of bioactive fractions...
February 13, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28188518/a-robust-protocol-for-protein-extraction-and-digestion
#4
Michelle Atallah, Mark R Flory, Parag Mallick
Proteins play a key role in all aspects of cellular homeostasis. Proteomics, the large-scale study of proteins, provides in-depth data on protein properties, including abundances and post-translational modification states, and as such provides a rich avenue for the investigation of biological and disease processes. While proteomic tools such as mass spectrometry have enabled exquisitely sensitive sample analysis, sample preparation remains a critical unstandardized variable that can have a significant impact on downstream data readouts...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28176961/blooming-gelatin-an-individual-additive-for-enhancing-nanoapatite-precipitation-physical-properties-and-osteoblastic-responses-of-nanostructured-macroporous-calcium-phosphate-bone-cements
#5
Ziba Orshesh, Saeed Hesaraki, Ali Khanlarkhani
In recent years, there has been a great interest in using natural polymers in the composition of calcium phosphate bone cements to enhance their physical, mechanical, and biological performance. Gelatin is a partially hydrolyzed form of collagen, a natural component of bone matrix. In this study, the effect of blooming gelatin on the nanohydroxyapatite precipitation, physical and mechanical properties, and cellular responses of a calcium phosphate bone cement (CPC) was investigated. Various concentrations of blooming gelatin (2, 5, and 8 wt...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28170017/quantitative-glycomics-using-liquid-phase-separations-coupled-to-mass-spectrometry
#6
REVIEW
Josh Smith, Stefan Mittermayr, Csaba Váradi, Jonathan Bones
Post-translational modification of proteins by the attachment of glycans is governed by a variety of highly specific enzymes and is associated with fundamental impacts on the parent protein's physical, chemical and biological properties. The inherent connection between cellular physiology and specific glycosylation patterns has been shown to offer potential for diagnostic and prognostic monitoring of altered glycosylation in the disease state. Conversely, glycoprotein based biopharmaceuticals have emerged as dominant therapeutic strategies in the treatment of intricate diseases...
February 7, 2017: Analyst
https://www.readbyqxmd.com/read/28164194/covalent-functionalization-of-polyhedral-graphitic-particles-synthesized-by-arc-discharge-from-graphite
#7
E Voss, B Vigolo, G Medjahdi, C Hérold, J-F Marêché, J Ghanbaja, F Le Normand, V Mamane
Carbon materials including carbon nanoparticles, such as nanographite, graphene and graphenic materials, and carbon nanotubes are known to be highly hydrophobic. Oxidation treatments are widely used as the best methods to improve their affinity in a liquid medium or a polymer matrix so that they can be dispersed, handled and processed. Here, we have applied eight different oxidation treatments in order to graft oxygen-containing functional groups at the surface of polyhedral graphitic particles synthesized by arc discharge from graphite, also called astralenes...
February 6, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28163783/a-framework-for-accelerated-phototrophic-bioprocess-development-integration-of-parallelized-microscale-cultivation-laboratory-automation-and-kriging-assisted-experimental-design
#8
Holger Morschett, Lars Freier, Jannis Rohde, Wolfgang Wiechert, Eric von Lieres, Marco Oldiges
BACKGROUND: Even though microalgae-derived biodiesel has regained interest within the last decade, industrial production is still challenging for economic reasons. Besides reactor design, as well as value chain and strain engineering, laborious and slow early-stage parameter optimization represents a major drawback. RESULTS: The present study introduces a framework for the accelerated development of phototrophic bioprocesses. A state-of-the-art micro-photobioreactor supported by a liquid-handling robot for automated medium preparation and product quantification was used...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28157331/condensation-and-demixing-in-solutions-of-dna-nanostars-and-their-mixtures
#9
Emanuele Locatelli, Philip H Handle, Christos N Likos, Francesco Sciortino, Lorenzo Rovigatti
We present a novel numerical/theoretical approach to efficiently evaluate the phase diagram of self-assembling DNA nanostars. Combining input information based on a realistic coarse-grained DNA potential with the Wertheim association theory we derive a parameter-free thermodynamic description of these systems. We apply this method to investigate the phase behaviour of single-component and mixtures of DNA nanostars with different number of sticky arms, elucidating the role of the system functionality and of salt concentration...
February 3, 2017: ACS Nano
https://www.readbyqxmd.com/read/28151671/contactless-transport-and-mixing-of-liquids-on-self-sustained-sublimating-coatings
#10
Athanasios Milionis, Carlo Antonini, Stefan Jung, Anders Nelson, Thomas M Schutzius, Dimos Poulikakos
Controlled handling of liquids and colloidal suspensions as they interact with surfaces, targeting a broad palette of related functionalities, is of great importance in science, technology, and nature. When small liquid volumes (drops on the order of microliters or nanoliters) need to be processed in microfluidic devices, contamination on the solid/liquid interface and loss of liquid due to adhesion on the transport channels are two very common problems that can significantly alter the process outcome, for example, the chemical reaction efficiency or the purity and the final concentration of a suspension...
February 13, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28138848/long-fragment-read-lfr-technology-cost-effective-high-quality-genome-wide-molecular-haplotyping
#11
Mark A McElwain, Rebecca Yu Zhang, Radoje Drmanac, Brock A Peters
In this chapter, we describe Long Fragment Read (LFR) technology, a DNA preprocessing method for genome-wide haplotyping by whole genome sequencing (WGS). The addition of LFR prior to WGS on any high-throughput DNA sequencer (e.g., Complete Genomics Revolocity™, BGISEQ-500, Illumina HiSeq, etc.) enables the assignment of single-nucleotide polymorphisms (SNPs) and other genomic variants onto contigs representing contiguous DNA from a single parent (haplotypes) with N50 lengths of up to ~1 Mb. Importantly, this is achieved independent of any parental sequencing data or knowledge of parental haplotypes...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28104718/boncat-enables-time-resolved-analysis-of-protein-synthesis-in-native-plant-tissue
#12
Weslee Glenn, Shannon E Stone, Samuel H Ho, Michael J Sweredoski, Annie Moradian, Sonja Hess, Julia Bailey-Serres, David A Tirrell
Proteomic plasticity undergirds stress responses in plants, and understanding such responses requires accurate measurement of the extent to which proteins levels are adjusted to counter external stimuli. Here, we adapt bioorthogonal non-canonical amino acid tagging (BONCAT) to interrogate protein synthesis in vegetative Arabidopsis thaliana seedlings. BONCAT relies on the translational incorporation of a non-canonical amino acid (ncAA) probe into cellular proteins. In this study, the probe is the methionine surrogate azidohomoalanine (Aha), which carries a reactive azide moiety in its amino acid side chain...
January 19, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28095176/a-flexible-workflow-for-automated-bioluminescent-kinase-selectivity-profiling
#13
Tracy Worzella, Matt Butzler, Jacquelyn Hennek, Seth Hanson, Laura Simdon, Said Goueli, Cris Cowan, Hicham Zegzouti
Kinase profiling during drug discovery is a necessary process to confirm inhibitor selectivity and assess off-target activities. However, cost and logistical limitations prevent profiling activities from being performed in-house. We describe the development of an automated and flexible kinase profiling workflow that combines ready-to-use kinase enzymes and substrates in convenient eight-tube strips, a bench-top liquid handling device, ADP-Glo Kinase Assay (Promega, Madison, WI) technology to quantify enzyme activity, and a multimode detection instrument...
November 1, 2016: Journal of Laboratory Automation
https://www.readbyqxmd.com/read/28093629/liquid-release-as-a-source-of-potential-drug-exposure-during-the-handling-of-intravenous-infusions-in-nursing
#14
Verena Segner, Renate Kimbel, Philipp Jochems, André Heinemann, Stephan Letzel, Daniel Wollschläger, Bernd Roßbach
PURPOSE: This study aims at experimentally determining the incidence and extent of liquid releases onto the operator's hands and into the work environment during common nursing operations involving infusions. METHODS: A sequence of operations related to the preparation and administration of infusions was conducted by three subjects for 15 times each using fluorescein marked infusion solutions and two different infusion sets (standard set vs. safety-optimized set)...
January 16, 2017: International Archives of Occupational and Environmental Health
https://www.readbyqxmd.com/read/28078439/liquid-based-versus-conventional-cytology-of-oral-brush-biopsies-a-split-sample-pilot-study
#15
T W Remmerbach, N Pomjanski, U Bauer, H Neumann
OBJECTIVE: The aim of this prospective split sample study was to evaluate the applicability of liquid-based cytology (LBC) of oral brush biopsies for detection of oral cancer. METHODS: Two different preparation techniques were investigated: the conventional transfer procedure to glass slides and the LBC preparation method. The obtainments of epithelial cells were performed five times with a nylon brush and transferred onto five glass slides. Additionally, the brushes, which were normally discarded, were stored in a fixative solution...
January 11, 2017: Clinical Oral Investigations
https://www.readbyqxmd.com/read/28074108/cellulolytic-robolector-towards-an-automated-high-throughput-screening-platform-for-recombinant-cellulase-expression
#16
Martina Mühlmann, Martin Kunze, Joaquim Ribeiro, Bertram Geinitz, Christian Lehmann, Ulrich Schwaneberg, Ulrich Commandeur, Jochen Büchs
BACKGROUND: Cellulases are key player in the hydrolyzation of cellulose. Unfortunately, this reaction is slow and a bottleneck in the process chain from biomass to intermediates and biofuels due to low activities of the enzymes. To overcome this draw back, a lot of effort is put into the area of protein engineering, to modify these enzymes by directed evolution or rational design. Huge clone libraries are constructed and have to be screened for improved variants. High-throughput screening is the method of choice to tackle this experimental effort, but up to now only a few process steps are adapted to automated platforms and little attention has been turned to the reproducibility of clone rankings...
2017: Journal of Biological Engineering
https://www.readbyqxmd.com/read/28054367/development-of-an-uplc-ms-ms-method-for-routine-therapeutic-drug-monitoring-of-aripiprazole-amisulpride-olanzapine-paliperidone-and-ziprasidone-with-a-discussion-of-their-therapeutic-reference-ranges-for-chinese-patients
#17
Shao-Ting Wang, Yan Li
A highly feasible and reliable ultra-high performance liquid chromatography tandem mass spectrometry method was presented for therapeutic drug monitoring of five anti-schizophrenic drugs (amisulpride, olanzapine, aripiprazole, paliperidone and ziprasidone) simultaneously. To meet the requirements of practical clinical usage (easy-handling, high-throughput and cost effective), the pretreatment process has been especially simplified (only including protein precipitation and mobile phase dilution steps) and the UPLC separation cycle was set within 6 minutes...
January 5, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28044289/digital-microfluidics-assisted-sealing-of-individual-magnetic-particles-in-femtoliter-sized-reaction-wells-for-single-molecule-detection
#18
Deborah Decrop, Elena Pérez Ruiz, Phalguni Tewari Kumar, Lisa Tripodi, Tadej Kokalj, Jeroen Lammertyn
Digital microfluidics has emerged in the last years as a promising liquid handling technology for a variety of applications. Here, we describe in detail how to build up an electrowetting-on-dielectric-based digital microfluidic chip with unique advantages for performing single-molecule detection. We illustrate how superparamagnetic particles can be printed with very high loading efficiency (over 98 %) and single-particle resolution in the microwell array patterned in the Teflon-AF(®) surface of the grounding plate of the chip...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28044285/capillary-driven-microfluidic-chips-for-miniaturized-immunoassays-patterning-capture-antibodies-using-microcontact-printing-and-dry-film-resists
#19
Yuksel Temiz, Robert D Lovchik, Emmanuel Delamarche
The miniaturization of immunoassays using microfluidic devices is attractive for many applications, but an important challenge remains the patterning of capture antibodies (cAbs) on the surface of microfluidic structures. Here, we describe how to pattern cAbs on planar poly(dimethylsiloxane) (PDMS) stamps and how to microcontact print the cAbs on a dry-film resist (DFR). DFRs are new types of photoresists having excellent chemical resistance and good mechanical, adhesive, and optical properties. Instead of being liquid photoresists, DFRs are thin layers that are easy to handle, cut, photo-pattern, and laminate over surfaces...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28034712/evaluation-of-a-digital-dispenser-for-direct-curve-dilutions-in-a-vaccine-potency-assay
#20
Christopher Roselle, Dana Whitehouse, Thy Follmer, Fran Ansbro, Silikhone Bouaraphan, Liming Guan, Sha-Ke Wang, Mary Shank-Retzlaff, Thorsten Verch
Dilutions are a common source of analytical error, both in terms of accuracy and precision, and a common source of analyst mistakes. When serial dilutions are used, errors compound, even when employing laboratory automation. Direct point dilutions instead of serial dilutions can reduce error but is often impractical as they require either large diluent volumes or very small sample volumes when performed with traditional liquid handling equipment. We evaluated preparation of dilution curves using a picoliter digital dispenser, the HP, Inc...
December 27, 2016: Journal of Immunological Methods
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