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Liquid Handling

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https://www.readbyqxmd.com/read/29335416/lack-of-pronounced-changes-in-the-expression-of-fatty-acid-handling-proteins-in-adipose-tissue-and-plasma%C3%A2-of-morbidly-obese-humans
#1
Ewa Anna Grzegorczyk, Ewa Harasim-Symbor, Bartlomiej Lukaszuk, Dorota Harasiuk, Barbara Choromanska, Piotr Mysliwiec, Malgorzata Zendzian-Piotrowska, Adrian Chabowski
BACKGROUND/OBJECTIVES: Fatty acid handling proteins are involved in the process of accumulation of lipids in different fat tissue depots. Thus, the aim of the study was to estimate the expression of both fatty acid transport and binding proteins in the subcutaneous (SAT) and visceral adipose tissue (VAT) of patients with morbid obesity without metabolic syndrome, as well as the plasma concentrations of these transporters. SUBJECTS/METHODS: Protein (Western blotting) and mRNA (Real-time PCR) expression of selected fatty acid handling proteins was assessed in the visceral and subcutaneous adipose tissue of 30 patients with morbid obesity...
January 15, 2018: Nutrition & Diabetes
https://www.readbyqxmd.com/read/29334653/depletion-study-withdrawal-period-calculation-and-bioaccumulation-of-sulfamethazine-in-tilapia-oreochromis-niloticus-treated-with-medicated-feed
#2
Kátia S D Nunes, José H Vallim, Márcia R Assalin, Sonia C N Queiroz, Lourival C Paraíba, Claudio M Jonsson, Felix G R Reyes
The residue depletion of sulfamethazine (SMZ) was evaluated in tilapia (Oreochromis niloticus) after 11 days of administration of medicated feed containing SMZ, at the dose of 422 mg/kg body weight (bw). The determination of SMZ in feed and tilapia fillet was carried out using the QuEChERS approach for sample preparation, and high performance liquid chromatography with diode array detector (HPLC-DAD) and ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QToF-MS) for quantitation, respectively...
January 9, 2018: Chemosphere
https://www.readbyqxmd.com/read/29334125/determination-of-isoorientin-levels-in-rat-plasma-after-oral-administration-of-vaccinum-bracteatum-thunb-methanol-extract-by-high-performance-liquid-chromatography-tandem-mass-spectrometry
#3
Min-Ji Kim, Seung-Hwan Kwon, Choon-Gon Jang, Han-Joo Maeng
A simple, sensitive and rapid liquid chromatography tandem mass spectrometry method (LC-MS/MS) was developed and validated for the determination of plasma isoorientin levels in rats. After simple protein precipitation using methanol, chromatographic analysis was performed using a Synergi 4μ polar-RP 80A column (150 mm × 2.0 mm, 4 μm) under isocratic conditions and a mobile phase consisting of 0.1% formic acid in water and methanol (80:20, v/v) at a flow rate of 0.2 mL/min. In positive electrospray ionization mode, the protonated precursor and product ion transitions of isoorientin (m/z 449...
January 15, 2018: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/29331859/development-of-a-method-to-determine-axitinib-lapatinib-and-afatinib-in-plasma-by-micellar-liquid-chromatography-and-validation-by-the-european-medicines-agency-guidelines
#4
Jaume Albiol-Chiva, Josep Esteve-Romero, Juan Peris-Vicente
A method based on micellar liquid chromatography to quantify the tyrosine kinase inhibitors axitinib, lapatinib and afatinib in plasma is reported. The sample pretreatment was a simple 1/5-dilution in a pure micellar solution, filtration and direct injection, without requiring extraction or purification steps. The three drugs were resolved from the matrix in 17min, using an aqueous solution of 0.07M sodium dodecyl sulfate - 6.0% 1-pentanol, buffered at pH7 with 0.01M phosphate salt as mobile phase, running under isocratic mode at 1mL/min through a C18 column...
January 2, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29329706/unattended-reaction-monitoring-using-an-automated-microfluidic-sampler-and-on-line-liquid-chromatography
#5
Darshan C Patel, Yaqi Fara Lyu, Jorge Gandarilla, Steve Doherty
In-process sampling and analysis is an important aspect of monitoring kinetic profiles and impurity formation or rejection, both in development and during commercial manufacturing. In pharmaceutical process development, the technology of choice for a substantial portion of this analysis is high-performance liquid chromatography (HPLC). Traditionally, the sample extraction and preparation for reaction characterization have been performed manually. This can be time consuming, laborious, and impractical for long processes...
April 3, 2018: Analytica Chimica Acta
https://www.readbyqxmd.com/read/29323498/scaling-laws-in-directional-spreading-of-droplets-on-wettability-confined-diverging-tracks
#6
Uddalok Sen, Souvick Chatterjee, Ranjan Ganguly, Richard Dodge, Lisha Yu, Constantine M Megaridis
Spontaneous pumpless transport of droplets on wettability-confined tracks is important for various applications, such as rapid transport and mixing of fluid droplets, enhanced dropwise condensation, biomedical devices, etc. Recent studies have shown that on an open surface, a superhydrophilic track of diverging width, laid on a superhydrophobic background, facilitates the transport of water from the narrower end to the wider end at unprecedented rates (up to 40 cm/s), without external actuation. Keeping in mind that such designs play a key role for a diverse range of applications handling organic liquids and in point-of-care devices, the importance of characterizing the spreading behavior of viscous liquids on such surfaces cannot be over-emphasized...
January 11, 2018: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/29320159/bioinspired-nanostructured-surfaces-for-on-demand-bubble-transportation
#7
Xin Tang, Hairui Xiong, Tiantian Kong, Ye Tian, Wen-Di Li, Liqiu Wang
The maneuver of small bubbles in a programmed way will advance numerous processes, including gas evolution reaction and aeration. Unlike in-air droplets, rapidly rising bubbles in liquid medium can hardly be steered through interaction with solid substrates, causing difficulties in maneuvering bubbles. We pattern and lubricate nanoporous substrates with regions of contrasting wettability that is similar to the back of Namib desert beetles and subsequently immerse the lubricated surface underwater to spontaneously form spatially patterned Nepenthes-inspired slippery surfaces after the dewetting of lubricants...
January 10, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29316103/easy-to-attach-detach-solubilizing-tag-aided-chemical-synthesis-of-an-aggregative-capsid-protein
#8
Shugo Tsuda, Masayoshi Mochizuki, Hiroyuki Ishiba, Kumiko Yoshizawa-Kumagaye, Hideki Nishio, Shinya Oishi, Taku Yoshiya
A solubilizing "Trt-K10" tag was developed for the effective chemical preparation of peptides/proteins with low solubility. The Trt-K10 tag comprises a hydrophilic oligo-Lys sequence and a trityl anchor, and can be selectively introduced to a side chain thiol of Cys of deprotected peptides/proteins with a trityl alcohol-type introducing reagent "Trt(OH)-K10" under acidic conditions. Significantly, the ligation product in the reaction mixture of a thiol-additive-free native chemical ligation can be modified directly in a one-pot manner to facilitate the isolation of the product by high-performance liquid chromatography...
January 6, 2018: Angewandte Chemie
https://www.readbyqxmd.com/read/29315917/covalent-bonding-of-thermoplastics-to-rubbers-for-printable-reel-to-reel-processing-in-soft-robotics-and-microfluidics
#9
Jay M Taylor, Karla Perez-Toralla, Ruby Aispuro, Stephen A Morin
The lamination of mechanically stiff structures to elastic materials is prevalent in biological systems and popular in many emerging synthetic systems, such as soft robotics, microfluidics, stretchable electronics, and pop-up assemblies. The disparate mechanical and chemical properties of these materials have made it challenging to develop universal synthetic procedures capable of reliably adhering to these classes of materials together. Herein, a simple and scalable procedure is described that is capable of covalently laminating a variety of commodity ("off-the-shelf") thermoplastic sheets to silicone rubber films...
January 8, 2018: Advanced Materials
https://www.readbyqxmd.com/read/29312846/role-of-fast-setting-cements-in-arthroplasty-a-comparative-analysis-of-characteristics
#10
Neil Ayron Caraan, Reinhard Windhager, Jason Webb, Nadine Zentgraf, Klaus-Dieter Kuehn
AIM: To evaluate the behaviour of two fast-setting polymethylmethacrylate (PMMA) cements CMW® 2G and Palacos® fast R + G, as reference: Standard-setting Palacos® R + G. METHODS: The fast-setting cements CMW® 2G and Palacos® fast R + G were studied, using standard-setting high viscosity Palacos® R + G as a reference. Eleven units (of two batch numbers) of each cement were tested. All cements were mixed as specified by the manufacturer and analysed on the following parameters: Handling properties (mixing, waiting, working and hardening phase) according to Kuehn, Mechanical properties according to ISO 5833 and DIN 53435, Fatigue strength according to ISO 16402, Benzoyl Peroxide (BPO) - Content by titration, powder/liquid-ratio by weighing, antibiotic elution profile by High Performance Liquid Chromatography...
December 18, 2017: World Journal of Orthopedics
https://www.readbyqxmd.com/read/29304047/16s-rrna-analysis-of-diversity-of-manure-microbial-community-in-dairy-farm-environment
#11
Pramod Pandey, Colleen Chiu, Max Miao, Yi Wang, Matthew Settles, Noelia Silva Del Rio, Alejandro Castillo, Alex Souza, Richard Pereira, Richard Jeannotte
Dairy farms generate a considerable amount of manure, which is applied in cropland as fertilizer. While the use of manure as fertilizer reduces the application of chemical fertilizers, the main concern with regards to manure application is microbial pollution. Manure is a reservoir of a broad range of microbial populations, including pathogens, which have potential to cause contamination and pose risks to public and animal health. Despite the widespread use of manure fertilizer, the change in microbial diversity of manure under various treatment processes is still not well-understood...
2018: PloS One
https://www.readbyqxmd.com/read/29302897/efficient-application-of-volatile-anaesthetics-total-rebreathing-or-specific-reflection
#12
REVIEW
Hagen Bomberg, Thomas Volk, Heinrich V Groesdonk, Andreas Meiser
The circle system has been in use for more than a 100 years, whereas the first clinical application of an anaesthetic reflector was reported just 15 years ago. Its functional basis relies on molecular sieves such as zeolite crystals or activated carbon. In a circle system, the breathing gas is rebreathed after carbon dioxide absorption; a reflector on the other hand specifically retains the anaesthetic during expiration and resupplies it during the next inspiration. Reflection systems can be used in conjunction with intensive care ventilators and do not need the permanent presence of trained qualified staff...
January 4, 2018: Journal of Clinical Monitoring and Computing
https://www.readbyqxmd.com/read/29300899/tissue-distribution-of-suvorexant-in-three-forensic-autopsy-cases
#13
Brian Waters, Kenji Hara, Natsuki Ikematsu, Mio Takayama, Aya Matsusue, Masayuki Kashiwagi, Shin-Ichi Kubo
Suvorexant (Belsomra®) is a relatively new insomnia medication that has been available in USA and Japan since 2014. It is a dual orexin receptor antagonist that promotes sleep by inhibiting the binding of orexin neurons to the OX1R and OX2R receptors. In this report, we describe the detection and quantitation of suvorexant from the postmortem specimens of three separate autopsy cases handled by our department. Suvorexant was identified by fast gas chromatography/mass spectrometry during routine screening, and quantitated by a fully validated liquid chromatography-tandem mass spectroscopy method...
December 28, 2017: Journal of Analytical Toxicology
https://www.readbyqxmd.com/read/29300769/validation-of-an-automated-system-for-aliquoting-of-hiv-1-env-pseudotyped-virus-stocks
#14
Anke Schultz, Anja Germann, Martina Fuss, Marcella Sarzotti-Kelsoe, Daniel A Ozaki, David C Montefiori, Heiko Zimmermann, Hagen von Briesen
The standardized assessments of HIV-specific immune responses are of main interest in the preclinical and clinical stage of HIV-1 vaccine development. In this regard, HIV-1 Env-pseudotyped viruses play a central role for the evaluation of neutralizing antibody profiles and are produced according to Good Clinical Laboratory Practice- (GCLP-) compliant manual and automated procedures. To further improve and complete the automated production cycle an automated system for aliquoting HIV-1 pseudovirus stocks has been implemented...
2018: PloS One
https://www.readbyqxmd.com/read/29300174/ionic-liquid-interface-at-an-electrode-simulations-of-electrochemical-properties-using-an-asymmetric-restricted-primitive-model
#15
Hongduo Lu, Sture Nordholm, Cliff Woodward, Jan Forsman
We use Monte Carlo simulations of a coarse-grained model to investigate structure and electrochemical behaviours at an electrode immersed in room temperature ionic liquids (RTILs). The simple RTIL model, which we denote the Asymmetric Restricted Primitive Model (ARPM), is composed of monovalent hard-sphere ions, all of the same size, in which the charge is asymmetrically placed. Not only the hard-sphere size, but also the charge displacement, is identical for all species, i.e...
January 4, 2018: Journal of Physics. Condensed Matter: An Institute of Physics Journal
https://www.readbyqxmd.com/read/29289328/online-lasil-laser-ablation-of-solid-samples-in-liquid-with-online-coupled-icp-oes-detection-for-direct-determination-of-the-stoichiometry-of-complex-metal-oxide-thin-layers
#16
Maximilian Bonta, Johannes Frank, Stefanie Taibl, Jürgen Fleig, Andreas Limbeck
Advanced materials such as complex metal oxides are used in a wide range of applications and have further promising perspectives in the form of thin films. The exact chemical composition essentially influences the electronic properties of these materials which makes correct assessment of their composition necessary. However, due to high chemical resistance and in the case of thin films low absolute analyte amounts, this procedure is in most cases not straightforward and extremely time-demanding. Commonly applied techniques either lack in ease of use (i...
February 13, 2018: Analytica Chimica Acta
https://www.readbyqxmd.com/read/29288358/an-update-on-coating-manufacturing-techniques-of-microneedles
#17
REVIEW
Tamara N Tarbox, Alan B Watts, Zhengrong Cui, Robert O Williams
Recently, results have been published for the first successful phase I human clinical trial investigating the use of dissolving polymeric microneedles… Even so, further clinical development represents an important hurdle that remains in the translation of microneedle technology to approved products. Specifically, the potential for accumulation of polymer within the skin upon repeated application of dissolving and coated microneedles, combined with a lack of safety data in humans, predicates a need for further clinical investigation...
December 29, 2017: Drug Delivery and Translational Research
https://www.readbyqxmd.com/read/29286407/generic-protocol-for-optimization-of-heterologous-protein-production-using-automated-microbioreactor-technology
#18
Johannes Hemmerich, Lars Freier, Wolfgang Wiechert, Eric von Lieres, Marco Oldiges
A core business in industrial biotechnology using microbial production cell factories is the iterative process of strain engineering and optimization of bioprocess conditions. One important aspect is the improvement of cultivation medium to provide an optimal environment for microbial formation of the product of interest. It is well accepted that the media composition can dramatically influence overall bioprocess performance. Nutrition medium optimization is known to improve recombinant protein production with microbial systems and thus, this is a rewarding step in bioprocess development...
December 15, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29286379/automated-robotic-liquid-handling-assembly-of-modular-dna-devices
#19
Luis Ortiz, Marilene Pavan, Lloyd McCarthy, Joshua Timmons, Douglas M Densmore
Recent advances in modular DNA assembly techniques have enabled synthetic biologists to test significantly more of the available "design space" represented by "devices" created as combinations of individual genetic components. However, manual assembly of such large numbers of devices is time-intensive, error-prone, and costly. The increasing sophistication and scale of synthetic biology research necessitates an efficient, reproducible way to accommodate large-scale, complex, and high throughput device construction...
December 1, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29283217/microbioreactor-systems-for-accelerated-bioprocess-development
#20
REVIEW
Johannes Hemmerich, Stephan Noack, Wolfgang Wiechert, Marco Oldiges
In recent years, microbioreactor (MBR) systems have evolved towards versatile bioprocess engineering tools. They provide a unique solution to combine higher experimental throughput with extensive bioprocess monitoring and control, which is indispensable to develop economically and ecologically competitive bioproduction processes. MBR systems are based either on down-scaled stirred tank reactors or on advanced shaken microtiter plate cultivation devices. Importantly, MBR systems make use of optical measurements for non-invasive, online monitoring of important process variables like biomass concentration, dissolved oxygen, pH and fluorescence...
December 28, 2017: Biotechnology Journal
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