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https://www.readbyqxmd.com/read/29223894/a-model-based-fish-bioassessment-index-for-eastern-mediterranean-rivers-application-in-a-biogeographically-diverse-area
#1
S Zogaris, V Tachos, A N Economou, Y Chatzinikolaou, N Koutsikos, S Schmutz
In ecosystems with high fish species endemicity, such as Mediterranean-type rivers, biogeographical differences among ecoregions present serious obstacles to developing broadly-applicable river bioassessment indices. This impediment has contributed to a serious time-lag in developing EU policy-relevant fish-based indices in the Eastern Mediterranean countries. Here we present the first model-based fish index for the Eastern Mediterranean (the Hellenic Fish Index, HeFI) in an effort to overcome biogeographic differences among the area's biotically heterogeneous rivers...
December 7, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/29223582/looking-for-relationship-between-the-populations-of-dothistroma-septosporum-in-northern-europe-and-asia
#2
Kalev Adamson, Martin S Mullett, Halvor Solheim, Irene Barnes, Michael M Müller, Jarkko Hantula, Martti Vuorinen, Audrius Kacergius, Svetlana Markovskaja, Dmitry L Musolin, Kateryna Davydenko, Nenad Keča, Karli Ligi, Rasa D Priedite, Hanna Millberg, Rein Drenkhan
Dothistroma septosporum, a notorious pine needle pathogen with an unknown historical geographic origin and poorly known distribution pathways, is nowadays found almost in all areas inhabited by pines (Pinus spp.). The main aim of this study was to determine the relationship between North European and East Asian populations. In total, 238 Eurasian D. septosporum isolates from 11 countries, including 211 isolates from northern Europe, 16 isolates from Russian Far East and 11 isolates from Bhutan were analysed using 11 species specific microsatellite and mating type markers...
December 6, 2017: Fungal Genetics and Biology: FG & B
https://www.readbyqxmd.com/read/29223568/n-3-oxododecanoyl-l-homoserine-lactone-a-quorum-sensing-molecule-affects-cellular-content-of-nonprotein-thiol-content-in-rat-lymphocytes-its-relation-with-intracellular-zn2
#3
Yumiko Nishimura-Danjobara, Keisuke Oyama, Kaori Kanemaru, Keiko Takahashi, Kumio Yokoigawa, Yasuo Oyama
Cellular actions of N-(3-oxododecanoyl)-l-homoserine-lactone (ODHL), a quorum sensing molecule of bacteria, were studied on rat thymocytes using a flow cytometer with appropriate fluorescent dyes to elucidate the effects of ODHL on host cells. A bell-shaped concentration-response relation was observed in the ODHL-induced changes in cellular glutathione content ([GSH]i). ODHL concentration-dependently increased intracellular Zn2+ levels ([Zn2+]i) and cellular O2- content ([O2-]i). The bell-shaped relation induced by ODHL can be explained as follows: a low concentration of ODHL is expected to induce moderate oxidative stress that intracellularly releases Zn2+ by converting thiols to disulfides...
December 6, 2017: Chemico-biological Interactions
https://www.readbyqxmd.com/read/29223331/the-nos-like-protein-from-the-microalgae-ostreococcus-tauri-is-a-genuine-and-ultrafast-no-producing-enzyme
#4
Marine Weisslocker-Schaetzel, François André, Nabila Touazi, Noelia Foresi, Mehdi Lembrouk, Pierre Dorlet, Annie Frelet-Barrand, Lorenzo Lamattina, Jérôme Santolini
The exponential increase of genomes' sequencing has revealed the presence of NO-Synthases (NOS) throughout the tree of life, uncovering an extraordinary diversity of genetic structure and biological functions. Although NO has been shown to be a crucial mediator in plant physiology, NOS sequences seem present solely in green algae genomes, with a first identification in the picoplankton species Ostreococcus tauri. There is no rationale so far to account for the presence of NOS in this early-diverging branch of the green lineage and its absence in land plants...
December 2017: Plant Science: An International Journal of Experimental Plant Biology
https://www.readbyqxmd.com/read/29219758/the-use-of-alternatives-to-clopidogrel-in-flow-diversion-treatment-with-the-pipeline-embolization-device
#5
Elias Atallah, Hassan Saad, Kimon Bekelis, Nohra Chalouhi, Stavropoula Tjoumakaris, David Hasan, Jorge Eller, David Stidd, Robert H Rosenwasser, Pascal Jabbour
OBJECTIVE Thromboembolic complications continue to be encountered with Pipeline embolization devices (PEDs) despite routine clopidogrel/aspirin antiplatelet therapy. This study examined the safety and efficacy of prasugrel in the management of clopidogrel-resistant patients treated for cerebral aneurysms. METHODS Four hundred thirty-seven consecutive patients were identified between January 2011 and May 2016. Patients allergic, or having less than 30% platelet inhibition, to a daily 75-mg dose of clopidogrel received 10 mg of prasugrel daily (n = 20) or 90 mg of ticagrelor twice daily (n = 2)...
December 8, 2017: Journal of Neurosurgery
https://www.readbyqxmd.com/read/29218863/population-structure-and-migration-of-the-tobacco-blue-mold-pathogen-peronospora-tabacina-into-north-america-and-europe
#6
Monica Blanco-Meneses, Ignazio Carbone, Jean B Ristaino
Tobacco blue mold, caused by Peronospora tabacina, is an oomycete plant pathogen that causes yearly epidemics in tobacco (Nicotiana tabacum) in the U.S. and Europe. The genetic structure of P. tabacina was examined in order to understand genetic diversity, population structure and patterns of migration. Two nuclear loci, Igs2 and Ypt1 and one mitochondrial locus, cox2, were amplified, cloned and sequenced from fifty four isolates of P. tabacina from the U.S., Central America-Caribbean-Mexico (CCAM), Europe and the Middle East (EULE)...
December 8, 2017: Molecular Ecology
https://www.readbyqxmd.com/read/29218079/vegf-c-mediated-enhancement-of-lymphatic-drainage-reduces-intestinal-inflammation-by-regulating-il-9-il-17-balance-and-improving-gut-microbiota-in-experimental-chronic-colitis
#7
Xiaolei Wang, Jin Zhao, Li Qin
BACKGROUND: Inflammation-associated lymphangiogenesis (IAL) induced by vascular endothelial growth factor (VEGF)-C/VEGF receptor-3 (VEGFR-3) pathway plays a crucial role in chronic intestinal inflammation. This study aimed to investigate the effects of VEGF-C mediated enhancement of lymphatic drainage on the intestinal inflammation in experimental chronic colitis (CC) and the potential mechanism was explored. METHODS: Mouse CC model was established by three cycles of 2% DSS administration for 5 days following water administration for 5 days...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/29216823/historical-isolation-and-contemporary-gene-flow-drive-population-diversity-of-the-brown-alga-sargassum-thunbergii-along-the-coast-of-china
#8
Jing-Jing Li, Zi-Min Hu, Zhong-Min Sun, Jian-Ting Yao, Fu-Li Liu, Pablo Fresia, De-Lin Duan
BACKGROUND: Long-term survival in isolated marginal seas of the China coast during the late Pleistocene ice ages is widely believed to be an important historical factor contributing to population genetic structure in coastal marine species. Whether or not contemporary factors (e.g. long-distance dispersal via coastal currents) continue to shape diversity gradients in marine organisms with high dispersal capability remains poorly understood. Our aim was to explore how historical and contemporary factors influenced the genetic diversity and distribution of the brown alga Sargassum thunbergii, which can drift on surface water, leading to long-distance dispersal...
December 7, 2017: BMC Evolutionary Biology
https://www.readbyqxmd.com/read/29214990/rna-sequencing-reveals-a-transcriptomic-portrait-of-human-mesenchymal-stem-cells-from-bone-marrow-adipose-tissue-and-palatine-tonsils
#9
Kyung-Ah Cho, Minhwa Park, Yu-Hee Kim, So-Youn Woo, Kyung-Ha Ryu
Human mesenchymal stem cells (MSCs) are adult multipotent cells that have plasticity and inhabit the stroma of diverse tissues. The potential utility of MSCs has been heavily investigated in the fields of regenerative medicine and cell therapy. However, MSCs represent diverse populations that may depend on the tissue of origin. Thus, the ability to identify specific MSC populations has remained difficult. Using RNA sequencing, we analyzed the whole transcriptomes of bone marrow-derived MSCs (BMs), adipose tissue-derived MSCs (AMs), and tonsil-derived MSCs (TMs)...
December 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29212942/factors-leading-to-the-loss-of-natural-elite-control-of-hiv-1-infection
#10
María Pernas, Laura Tarancón-Diez, Esther Rodríguez-Gallego, Josep Gómez, Julia G Prado, Concepción Casado, Beatriz Dominguez-Molina, Isabel Olivares, Maite Coiras, Agathe León, Carmen Rodriguez, Jose Miguel Benito, Norma Rallón, Montserrat Plana, Onofre Martinez-Madrid, Marta Dapena, Jose Antonio Iribarren, Jorge Del Romero, Felipe García, José Alcamí, M Ángeles Muñoz-Fernández, Francisco Vidal, Manuel Leal, Cecilio Lopez-Galindez, Ezequiel Ruiz-Mateos
HIV-1 elite controllers (EC) maintain undetectable viral load (VL) in the absence of antiretroviral treatment. However, these subjects have heterogeneous clinical outcomes including a proportion loosing HIV-1 control over time. In this work we compared, in a longitudinal design, transient EC, analyzed before and after the loss of virological control, versus persistent EC. The aim was to identify factors leading to the loss of natural virological control of HIV-1-infection with a longitudinal retrospective study design...
December 6, 2017: Journal of Virology
https://www.readbyqxmd.com/read/29212722/first-satellite-tracks-of-south-atlantic-sea-turtle-lost-years-seasonal-variation-in-trans-equatorial-movement
#11
Katherine L Mansfield, Milagros L Mendilaharsu, Nathan F Putman, Maria A G Dei Marcovaldi, Alexander E Sacco, Gustave Lopez, Thais Pires, Yonat Swimmer
In the South Atlantic Ocean, few data exist regarding the dispersal of young oceanic sea turtles. We characterized the movements of laboratory-reared yearling loggerhead turtles from Brazilian rookeries using novel telemetry techniques, testing for differences in dispersal during different periods of the sea turtle hatching season that correspond to seasonal changes in ocean currents. Oceanographic drifters deployed alongside satellite-tagged turtles allowed us to explore the mechanisms of dispersal (passive drift or active swimming)...
December 6, 2017: Proceedings. Biological Sciences
https://www.readbyqxmd.com/read/29211861/spontaneous-microguidewire-extrusion-from-the-foot-following-cerebral-aneurysm-treatment-first-case-report
#12
Kurdow Nader, Saleh Lamin, Joseph Leyon, Swarupsinh Chavda, Allan Thomas, Sarin Kuruvath
BACKGROUND AND IMPORTANCE: Microguidewire entrapment and failure related to endovascular therapy is a rare complication with limited case reports available, most of which are from cardiac endovascular intervention. Migration of the microguidewire has been reported in coronary intervention with some resulting in severe complications, but there are no reported cases of spontaneous extrusion of wire fragment following a neurointerventional procedure. We report the first case of a microguidewire entrapment with subsequent fracture, followed by spontaneous extrusion of the wire fragment...
November 29, 2017: Operative Neurosurgery (Hagerstown, Md.)
https://www.readbyqxmd.com/read/29209817/analysis-of-road-development-and-associated-agricultural-land-use-change
#13
Hakan Alphan
Development of road network is one of the strongest drivers of habitat fragmentation. It interferes with ecological processes that are based on material and energy flows between landscape patches. Therefore, changes in temporal patterns of roads may be regarded as important landscape-level environmental indicators. The aim of this study is to analyze road development and associated agricultural land use change near the town of Erdemli located in the eastern Mediterranean coast of Turkey. The study area has witnessed an unprecedented development of agriculture since the 2000s...
December 5, 2017: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/29209348/phylogeography-study-of-the-siberian-apricot-prunus-sibirica-l-in-northern-china-assessed-by-chloroplast-microsatellite-and-dna-makers
#14
Zhe Wang, Yanfei Zeng, Zhendong Zhang, Songbai Sheng, Ju Tian, Rongling Wu, Xiaoming Pang
There is evidence that a band of dry climate separated plants in East Asia into distinct northern and southern groups. However, few studies have focused on the arid belt in this region, especially with regard to plants. We analyzed genetic variation in 22 populations of Siberian apricot (Prunus sibirica L.), a temperate deciduous species distributed in this arid belt, using two chloroplast DNA (cpDNA) sequences, seven chloroplast microsatellite loci (cpSSRs), and 31 nuclear microsatellite loci (nSSRs), to study its phylogeography...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29205516/a-tripartite-mode-of-action-approach-for-investigating-the-impact-of-aneugens-on-tubulin-polymerization
#15
Valerie Stock, Andreas Sutter, Marian Raschke, Nina Queisser
Chemical-induced disruption of the cellular microtubule network is one key mechanism of aneugenicity. Since recent data indicate that genotoxic effects of aneugens show nonlinear dose-response relationships, margins of safety can be derived with the ultimate goal to perform a risk assessment for the support of drug development. Furthermore, microtubule-interacting compounds are widely used for cancer treatment. While there is a need to support the risk assessment of tubulin-interacting chemicals using reliable mechanistic assays, no standard assays exist to date in regulatory genotoxicity testing for the distinction of aneugenic mechanisms...
December 4, 2017: Environmental and Molecular Mutagenesis
https://www.readbyqxmd.com/read/29205172/a-data-driven-approach-for-addressing-the-lack-of-flow-waveform-data-in-studies-of-cerebral-arterial-flow-in-older-adults
#16
Michael J Durka, Isaac Hong Wong, David F Kallmes, Dario Pasalic, Fernando Mut, Manoj Jagani, Pablo J Blanco, Juan R Cebral, Anne M Robertson
Blood flow waveforms - essential data for hemodynamic modeling - are often in practice unavailable to researchers. The objectives of this work were to assess the variability among the waveforms for a clinically relevant older population, and develop data-based methods for addressing the missing waveform data for hemodynamic studies. Approach: We analyzed 272 flow waveforms from the internal carotid arteries of older patients (73±13 years) with moderate cardiovascular disease, and used these data to develop approaches to guide new approaches for hemodynamic studies...
December 5, 2017: Physiological Measurement
https://www.readbyqxmd.com/read/29204985/comparison-of-the-characteristics-of-macrophages-derived-from-murine-spleen-peritoneal-cavity-and-bone-marrow
#17
Yan-Long Zhao, Pu-Xun Tian, Feng Han, Jin Zheng, Xin-Xin Xia, Wu-Jun Xue, Xiao-Ming Ding, Chen-Guang Ding
Macrophages have a diverse set of functions based upon their activation states. The activation states, including resting (M0) and polarizing (M1 and M2) states, of macrophages derived from the mouse bone marrow, spleen, and peritoneal cavity (BMs, SPMs, and PCMs, respectively) were compared. We evaluated the macrophage yield per mouse and compared the surface markers major histocompatibility complex (MHC) II and CD86 by flow cytometry. The relative mRNA levels of tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, mannose receptor (MR), and Ym1 in the M0, M1, and M2 states were also compared using real-time polymerase chain reaction (PCR) analysis...
2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/29204037/staged-multimodality-treatment-of-a-large-ruptured-fusiform-supraclinoid-internal-carotid-artery-aneurysm-microsurgical-clip-assisted-endovascular-coiling
#18
Dale Ding, Thomas J Buell, Ching-Jen Chen, Daniel M Raper, Kenneth C Liu, Dennis G Vollmer
In the contemporary era of aneurysm management, large fusiform aneurysms presenting with subarachnoid hemorrhage (SAH) remain particularly challenging lesions to successfully manage. We describe a staged, multimodal treatment strategy for a 71-year-old patient who presented with a large ruptured fusiform aneurysm of the supraclinoid internal carotid artery (ICA) and a fetal posterior communicating artery which originated from the inferomedial aspect of the aneurysm. In the first stage, we performed a partial microsurgical clip reconstruction of the fusiform aneurysm and secured its rupture site, which was identified intraoperatively...
October 2017: Journal of Neurosciences in Rural Practice
https://www.readbyqxmd.com/read/29203907/the-sensitivity-and-stability-of-bacterioplankton-community-structure-to-wind-wave-turbulence-in-a-large-shallow-eutrophic-lake
#19
Jian Zhou, Boqiang Qin, Xiaoxia Han, Decai Jin, Zhiping Wang
Lakes are strongly influenced by wind-driven wave turbulence. The direct physical effects of turbulence on bacterioplankton community structure however, have not yet been addressed and remains poorly understood. To examine the stability of bacterioplankton communities under turbulent conditions, we simulated conditions in the field to evaluate the responses of the bacterioplankton community to physical forcing in Lake Taihu, using high-throughput sequencing and flow cytometry. A total of 4,520,231 high quality sequence reads and 74,842 OTUs were obtained in all samples with α-proteobacteria, γ-proteobacteria and Actinobacteria being the most dominant taxa...
December 4, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29203894/combining-intratumoral-treg-depletion-with-androgen-deprivation-therapy-adt-preclinical-activity-in-the-myc-cap-model
#20
Ying-Chun Shen, Ali Ghasemzadeh, Christina M Kochel, Thomas R Nirschl, Brian J Francica, Zoila A Lopez-Bujanda, Maria A Carrera Haro, Ada Tam, Robert A Anders, Mark J Selby, Alan J Korman, Charles G Drake
BACKGROUND: Immune checkpoint blockade has shown promising antitumor activity against a variety of tumor types. However, responses in castration-resistant prostate cancer remain relatively rare-potentially due to low baseline levels of infiltration. Using an immunocompetent cMyc-driven model (Myc-CaP), we sought to understand the immune infiltrate induced by androgen deprivation therapy (ADT) and to leverage that infiltration toward therapeutic benefit. METHODS: Using flow cytometry, qPCR and IHC, we quantified ADT-induced immune infiltration in terms of cell type and function...
December 4, 2017: Prostate Cancer and Prostatic Diseases
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