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https://www.readbyqxmd.com/read/28546895/cvd-transfer-free-graphene-for-sensing-applications
#1
Chiara Schiattarella, Sten Vollebregt, Tiziana Polichetti, Brigida Alfano, Ettore Massera, Maria Lucia Miglietta, Girolamo Di Francia, Pasqualina Maria Sarro
The sp(2) carbon-based allotropes have been extensively exploited for the realization of gas sensors in the recent years because of their high conductivity and large specific surface area. A study on graphene that was synthetized by means of a novel transfer-free fabrication approach and is employed as sensing material is herein presented. Multilayer graphene was deposited by chemical vapour deposition (CVD) mediated by CMOS-compatible Mo. The utilized technique takes advantage of the absence of damage or contamination of the synthesized graphene, because there is no need for the transfer onto a substrate...
2017: Beilstein Journal of Nanotechnology
https://www.readbyqxmd.com/read/28545848/identification-of-immiscible-napl-contaminant-sources-in-aquifers-by-a-modified-two-level-saturation-based-imperialist-competitive-algorithm
#2
H R Ghafouri, M Mosharaf-Dehkordi, B Afzalan
A simulation-optimization model is proposed for identifying the characteristics of local immiscible NAPL contaminant sources inside aquifers. This model employs the UTCHEM 9.0 software as its simulator for solving the governing equations associated with the multi-phase flow in porous media. As the optimization model, a novel two-level saturation based Imperialist Competitive Algorithm (ICA) is proposed to estimate the parameters of contaminant sources. The first level consists of three parallel independent ICAs and plays as a pre-conditioner for the second level which is a single modified ICA...
April 29, 2017: Journal of Contaminant Hydrology
https://www.readbyqxmd.com/read/28545225/state-of-art-of-cancer-pharmacogenomics-in-latin-american-populations
#3
REVIEW
Andrés López-Cortés, Santiago Guerrero, María Ana Redal, Angel Tito Alvarado, Luis Abel Quiñones
Over the past decades, several studies have shown that tumor-related somatic and germline alterations predicts tumor prognosis, drug response and toxicity. Latin American populations present a vast geno-phenotypic diversity due to the great interethnic and interracial mixing. This genetic flow leads to the appearance of complex characteristics that allow individuals to adapt to endemic environments, such as high altitude or extreme tropical weather. These genetic changes, most of them subtle and unexplored, could establish a mutational profile to develop new pharmacogenomic therapies specific for Latin American populations...
May 23, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28545218/temporal-variability-in-taxonomic-and-trait-compositions-of-invertebrate-assemblages-in-two-climatic-regions-with-contrasting-flow-regimes
#4
Sylvain Dolédec, Jessica Tilbian, Núria Bonada
CONTEXT: Understanding natural temporal changes in Mediterranean rivers with contrasting flow regimes in relation to those of temperate rivers may prove helpful in predicting effects of climate change on aquatic biodiversity. GOAL: We aimed to compare temporal variability in taxonomic and trait compositions of nearly natural rivers in two climatic regions with varying flow regimes to address the effects of future climate changes on aquatic biodiversity in reference conditions...
May 21, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28544160/a-unified-continuous-flow-assembly-line-synthesis-of-highly-substituted-pyrazoles-and-pyrazolines
#5
Joshua Britton, Timothy F Jamison
A rapid and modular continuous flow synthesis of highly functionalized fluorinated pyrazoles and pyrazolines has been developed. Flowing fluorinated amines through sequential reactor coils mediates diazoalkane formation and [3+2] cycloaddition to generate more than 30 azoles in a telescoped fashion. Pyrazole cores are then sequentially modified through additional reactor modules performing N-alkylation and arylation, deprotection, and amidation to install broad molecular diversity in short order. Continuous flow synthesis enables the safe handling of diazoalkanes at elevated temperatures, and the use of aryl alkyne dipolarphiles under catalyst free conditions...
May 25, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28544069/cd38-enhances-the-proliferation-and-inhibits-the-apoptosis-of-cervical-cancer-cells-by-affecting-the-mitochondria-functions
#6
Shan Liao, Songshu Xiao, Hongxiang Chen, Manying Zhang, Zhifang Chen, Yuehua Long, Lu Gao, Guangchao Zhu, Junyu He, Shuping Peng, Wei Xiong, Zhaoyang Zeng, Zheng Li, Ming Zhou, Xiaoling Li, Jian Ma, Minghua Wu, Juanjuan Xiang, Guiyuan Li, Yanhong Zhou
Cervical cancer is one of the most common malignant tumors in women all over the world. The exact mechanism of occurrence and development of cervical cancer has not been fully elucidated. CD38 is a type II transmembrane glycoprotein, which was found to mediate diverse activities, including signal transduction, cell adhesion, and cyclic ADP-ribose synthesis. Here, we reported that CD38 promoted cell proliferation and inhibited cell apoptosis in cervical cancer cells by affecting the mitochondria functions. We established stable cervical cancer cell lines with CD38 over-expressed...
May 23, 2017: Molecular Carcinogenesis
https://www.readbyqxmd.com/read/28543189/rapid-radiations-of-both-kiwifruit-hybrid-lineages-and-their-parents-shed-light-on-a-two-layer-mode-of-species-diversification
#7
Yifei Liu, Dawei Li, Qiong Zhang, Chi Song, Caihong Zhong, Xudong Zhang, Ying Wang, Xiaohong Yao, Zupeng Wang, Shaohua Zeng, Ying Wang, Yangtao Guo, Shuaibin Wang, Xinwei Li, Li Li, Chunyan Liu, Honour C McCann, Weiming He, Yan Niu, Min Chen, Liuwen Du, Junjie Gong, Paul M Datson, Elena Hilario, Hongwen Huang
Reticulate speciation caused by interspecific hybridization is now recognized as an important mechanism in the creation of biological diversity. However, depicting the patterns of phylogenetic networks for lineages that have undergone interspecific gene flow is challenging. Here we sequenced 25 taxa representing natural diversity in the genus Actinidia with an average mapping depth of 26× on the reference genome to reconstruct their reticulate history. We found evidence, including significant gene tree discordance, cytonuclear conflicts, and changes in genome-wide heterozygosity across taxa, collectively supporting extensive reticulation in the genus...
May 25, 2017: New Phytologist
https://www.readbyqxmd.com/read/28543070/tnf-%C3%AE-has-both-stimulatory-and-inhibitory-effects-on-mouse-monocyte-derived-osteoclastogenesis
#8
Yixuan Cao, Ineke D C Jansen, Sara Sprangers, Teun J de Vries, Vincent Everts
Phenotypically different osteoclasts may be generated from different subsets of precursors. To what extent the formation of these osteoclasts is influenced or mediated by the inflammatory cytokine TNF-α, is unknown and was investigated in this study. The osteoclast precursors early blasts (CD31(hi) Ly-6C(-) ), myeloid blasts (CD31(+) Ly-6C(+) ) and monocytes (CD31(-) Ly-6C(hi) ) were sorted from mouse bone marrow using flow cytometry and cultured with M-CSF and RANKL, with or without TNF-α. Surprisingly, TNF-α prevented the differentiation of TRAcP(+) osteoclasts generated from monocytes on plastic; an effect not seen with early blasts and myeloid blasts...
May 20, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28543048/estimating-demographic-contributions-to-effective-population-size-in-an-age-structured-wild-population-experiencing-environmental-and-demographic-stochasticity
#9
Amanda E Trask, Eric M Bignal, Davy I McCracken, Stuart B Piertney, Jane M Reid
1.A population's effective size (Ne ) is a key parameter that shapes rates of inbreeding and loss of genetic diversity, thereby influencing evolutionary processes and population viability. However estimating Ne , and identifying key demographic mechanisms that underlie the Ne to census population size (N) ratio, remains challenging, especially for small populations with overlapping generations and substantial environmental and demographic stochasticity and hence dynamic age-structure. 2.A sophisticated demographic method of estimating Ne /N, which uses Fisher's reproductive value to account for dynamic age-structure, has been formulated...
May 24, 2017: Journal of Animal Ecology
https://www.readbyqxmd.com/read/28542462/a-human-monocytic-nf-%C3%AE%C2%BAb-fluorescent-reporter-cell-line-for-detection-of-microbial-contaminants-in-biological-samples
#10
Claire Battin, Annika Hennig, Patrick Mayrhofer, Renate Kunert, Gerhard J Zlabinger, Peter Steinberger, Wolfgang Paster
Sensing of pathogens by innate immune cells is essential for the initiation of appropriate immune responses. Toll-like receptors (TLRs), which are highly sensitive for various structurally and evolutionary conserved molecules derived from microbes have a prominent role in this process. TLR engagement results in the activation of the transcription factor NF-κB, which induces the expression of cytokines and other inflammatory mediators. The exquisite sensitivity of TLR signalling can be exploited for the detection of bacteria and microbial contaminants in tissue cultures and in protein preparations...
2017: PloS One
https://www.readbyqxmd.com/read/28541553/occurrence-of-15-haplotypes-of-linepithema-micans-hymenoptera-formicidae-in-southern-brazil
#11
Manuela Oliveira Ramalho, C Martins, T Campos, A Nondillo, M Botton, O C Bueno
The ant genus Linepithema is widely known, thanks to the pest species Linepithema humile (Mayr), which is easily mistaken for Linepithema micans (Forel) due to their morphological similarity. Like L. humile, L. micans is associated to the main grapevine pest in Brazil, Eurhizococcus brasiliensis (Wille), also known as ground pearl. Therefore, the present study uses mtDNA fragments to expand the knowledge of haplotype diversity and distribution of L. micans in the state of Rio Grande do Sul (Brazil), to understand the genetic differences of the populations identified in this study...
May 25, 2017: Journal of Economic Entomology
https://www.readbyqxmd.com/read/28539585/natural-convection-flow-of-a-nanofluid-in-an-inclined-square-enclosure-partially-filled-with-a-porous-medium
#12
A I Alsabery, A J Chamkha, H Saleh, I Hashim
This work analyses free convection flow of a nanofluid in an inclined square enclosure consisting of a porous layer and a nanofluid layer using the finite difference methodology. Sinusoidal temperature boundary conditions are imposed on the two opposing vertical walls. Nanofluids with water as base and Ag or Cu or Al2O3 or TiO2 nanoparticles are considered for the problem. The related parameters of this study are the Darcy number, nanoparticle volume fraction, phase deviation, amplitude ratio, porous layer thickness and the inclination angle of the cavity...
May 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28535882/versatile-fabrication-of-paper-based-microfluidic-devices-with-high-chemical-resistance-using-scholar-glue-and-magnetic-masks
#13
Thiago M G Cardoso, Fabrício R de Souza, Paulo T Garcia, Denilson Rabelo, Charles S Henry, Wendell K T Coltro
Simple methods have been developed for fabricating microfluidic paper-based analytical devices (μPADs) but few of these devices can be used with organic solvents and/or aqueous solutions containing surfactants. This study describes a simple fabrication strategy for μPADs that uses readily available scholar glue to create the hydrophobic flow barriers that are resistant to surfactants and organic solvents. Microfluidic structures were defined by magnetic masks designed with either neodymium magnets or magnetic sheets to define the patter, and structures were created by spraying an aqueous solution of glue on the paper surface...
June 29, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28533979/a-genome-wide-assessment-of-stages-of-elevational-parapatry-in-bornean-passerine-birds-reveals-no-introgression-implications-for-processes-and-patterns-of-speciation
#14
Robert G Moyle, Joseph D Manthey, Peter A Hosner, Mustafa Rahman, Maklarin Lakim, Frederick H Sheldon
Topographically complex regions often contain the close juxtaposition of closely related species along elevational gradients. The evolutionary causes of these elevational replacements, and thus the origin and maintenance of a large portion of species diversity along elevational gradients, are usually unclear because ecological differentiation along a gradient or secondary contact following allopatric diversification can produce the same pattern. We used reduced representation genomic sequencing to assess genetic relationships and gene flow between three parapatric pairs of closely related songbird taxa (Arachnothera spiderhunters, Chloropsis leafbirds, and Enicurus forktails) along an elevational gradient in Borneo...
2017: PeerJ
https://www.readbyqxmd.com/read/28533899/genetic-diversity-and-structure-of-an-endangered-desert-shrub-and-the-implications-for-conservation
#15
Zhihao Su, Bryce A Richardson, Li Zhuo, Xiaolong Jiang, Wenjun Li, Xiaoshan Kang
Population genetic information can provide valuable insight for the conservation and management of threatened and endangered plant species. Tamarix taklamakanensis is an endangered shrub endemic to arid basins of northwestern China. This species serves to stabilize soils in this region, but has seen substantial loss in its abundance due to depletion of ground water. The populations of this species have become small and fragmented, warranting conservation. Seven microsatellite loci were used to assess the genetic diversity and structure of 15 populations in the Tarim Basin, China...
May 2017: AoB Plants
https://www.readbyqxmd.com/read/28533084/gene-flow-and-genetic-drift-contribute-to-high-genetic-diversity-with-low-phylogeographical-structure-in-european-hoopoes-upupa-epops
#16
Erjia Wang, Rien E Van Wijk, Markus Santhosh Braun, Michael Wink
The Hoopoe (Upupa epops epops) breeds widely in Eurasia and most populations migrate to Africa during the boreal winter. To date, data regarding its phylogeography in Europe are missing. In this study, we investigated the phylogeography and population genetics of Hoopoes by means of mitochondrial DNA (mtDNA) sequencing as well as microsatellite genotyping. Our analyses revealed 32 haplotypes in the cytochrome c oxidase subunit I (COI) (269 individuals) and 50 haplotypes in cytochrome b (cyt b) (233 individuals)...
May 19, 2017: Molecular Phylogenetics and Evolution
https://www.readbyqxmd.com/read/28531960/multiple-stress-response-of-lowland-stream-benthic-macroinvertebrates-depends-on-habitat-type
#17
Daniel Graeber, Tinna M Jensen, Jes J Rasmussen, Tenna Riis, Peter Wiberg-Larsen, Annette Baattrup-Pedersen
Worldwide, lowland stream ecosystems are exposed to multiple anthropogenic stress due to the combination of water scarcity, eutrophication, and fine sedimentation. The understanding of the effects of such multiple stress on stream benthic macroinvertebrates has been growing in recent years. However, the interdependence of multiple stress and stream habitat characteristics has received little attention, although single stressor studies indicate that habitat characteristics may be decisive in shaping the macroinvertebrate response...
May 17, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28529720/recent-advances-in-metacommunities-and-meta-ecosystem-theories
#18
REVIEW
Frédéric Guichard
Metacommunity theory has provided many insights into the general problem of local versus regional control of species diversity and relative abundance. The metacommunity framework has been extended from competitive interactions to whole food webs that can be described as spatial networks of interaction networks. Trophic metacommunity theory greatly contributed to resolving the community complexity-stability debate by predicting its dependence on the regional spatial context. The meta-ecosystem framework has since been suggested as a useful simplification of complex ecosystems to apply this spatial context to spatial flows of both individuals and matter...
2017: F1000Research
https://www.readbyqxmd.com/read/28528630/correlation-of-live-cell-imaging-with-volume-scanning-electron-microscopy
#19
Miriam S Lucas, Maja Günthert, Anne Greet Bittermann, Alex de Marco, Roger Wepf
Live-cell imaging is one of the most widely applied methods in live science. Here we describe two setups for live-cell imaging, which can easily be combined with volume SEM for correlative studies. The first procedure applies cell culture dishes with a gridded glass support, which can be used for any light microscopy modality. The second approach is a flow-chamber setup based on Ibidi μ-slides. Both live-cell imaging strategies can be followed up with serial blockface- or focused ion beam-scanning electron microscopy...
2017: Methods in Cell Biology
https://www.readbyqxmd.com/read/28528621/using-lean-to-rapidly-and-sustainably-transform-a-behavioral-health-crisis-program-impact-on-throughput-and-safety
#20
Margaret E Balfour, Kathleen Tanner, Paul J Jurica, Dawn Llewellyn, Robert G Williamson, Chris A Carson
BACKGROUND: Lean has been increasingly applied in health care to reduce waste and improve quality, particularly in fast-paced and high-acuity clinical settings such as emergency departments. In addition, Lean's focus on engagement of frontline staff in problem solving can be a catalyst for organizational change. In this study, ConnectionsAZ demonstrates how they applied Lean principles to rapidly and sustainably transform clinical operations in a behavioral health crisis facility. METHODS: A multidisciplinary team of management and frontline staff defined values-based outcome measures, mapped the current and ideal processes, and developed new processes to achieve the ideal...
June 2017: Joint Commission Journal on Quality and Patient Safety
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