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https://www.readbyqxmd.com/read/27906050/the-sh3-and-cysteine-rich-domain-3-stac3-gene-is-important-to-growth-fiber-composition-and-calcium-release-from-the-sarcoplasmic-reticulum-in-postnatal-skeletal-muscle
#1
Xiaofei Cong, Jonathan Doering, Davi A G Mazala, Eva R Chin, Robert W Grange, Honglin Jiang
BACKGROUND: The SH3 and cysteine-rich domain 3 (Stac3) gene is specifically expressed in the skeletal muscle. Stac3 knockout mice die perinatally. In this study, we determined the potential role of Stac3 in postnatal skeletal muscle growth, fiber composition, and contraction by generating conditional Stac3 knockout mice. METHODS: We disrupted the Stac3 gene in 4-week-old male mice using the Flp-FRT and tamoxifen-inducible Cre-loxP systems. RESULTS: RT-qPCR and western blotting analyses of the limb muscles of target mice indicated that nearly all Stac3 mRNA and more than 70 % of STAC3 protein were deleted 4 weeks after tamoxifen injection...
April 11, 2016: Skeletal Muscle
https://www.readbyqxmd.com/read/27893858/reversing-age-related-changes-of-the-laryngeal-muscles-by-chronic-electrostimulation-of-the-recurrent-laryngeal-nerve
#2
Michael Karbiener, Jonathan C Jarvis, Justin D Perkins, Hermann Lanmüller, Martin Schmoll, Hanna S Rode, Claus Gerstenberger, Markus Gugatschka
Age related atrophy of the laryngeal muscles -mainly the thyroarytenoid muscle (TAM)- leads to a glottal gap and consequently to a hoarse and dysphonic voice that significantly affects quality of life. The aim of our study was to reverse this atrophy by inducing muscular hypertrophy by unilateral functional electrical stimulation (FES) of the recurrent laryngeal nerve (RLN) in a large animal model using aged sheep (n = 5). Suitable stimulation parameters were determined by fatiguing experiments of the thyroarytenoid muscle in an acute trial...
2016: PloS One
https://www.readbyqxmd.com/read/27889602/toxicity-evaluation-and-initial-characterization-of-the-venom-of-a-colombian-latrodectus-sp
#3
Alexandra Rueda, Emilio Realpe, Alfredo Uribe
The genus Latrodectus has not been studied in Colombia even though it is medically important worldwide; there are three species for the country, this study focused on a non-identified species found in the Tatacoa Desert in the Huila Department. This research is the first approximation to the extraction, composition analysis and toxicity evaluation of the venom of a species of the genus Latrodectus in Colombia; and aims to evaluate the toxicity by the initial characterization of its venom. The venom extraction was accomplished with electrostimulation and total protein concentration was determined by the Lowry method and BCA assays from crude venom; with these methods, high protein concentration of the samples was measured...
November 23, 2016: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/27876742/-on-the-problem-of-voice-rehabilitation-in-the-case-of-unilateral-vocal-fold-paresis
#4
V V Shilenkova
BACKGROUND: Unilateral vocal fold paresis (UVFP) significantly deteriorates the patient's quality of life. The leading role in the rehabilitation of the patients presenting with UVFP belongs to stimulation therapy which consists in the activation of muscle re-innervation of the larynx and the compensatory mechanisms allowing to achieve the most complete closure of the vocal folds during phonation. AIM: The objective of the present study was the optimization of the conservative treatment of patients with UVFP...
2016: Vestnik Otorinolaringologii
https://www.readbyqxmd.com/read/27857035/recovery-of-long-term-paresis-following-resection-of-who-grade-ii-gliomas-infiltrating-the-pyramidal-pathway
#5
Juan Martino, Hugo Caballero, Enrique M DE Lucas, Rousinelle DA Silva-Freitas, Carlos Velasquez, Elsa Gomez, Javier Vazquez-Bourgon, Alfonso Vázquez-Barquero
Recent publications had reported high rates of preoperative neurological impairments in WHO grade II gliomas (GIIG) that significantly affect the quality of life. Consequently, one step further in the analysis of surgical outcome in GIIG is to evaluate if surgery is capable to improve preoperative deficits. Here are reported two cases of GIIG infiltrating the primary motor cortex and pyramidal pathway that had a long-term paresis before surgery. Both patients were operated with intraoperative electrical stimulation mapping, with identification and preservation of the primary motor cortex and pyramidal tract...
February 2017: Journal of Neurosurgical Sciences
https://www.readbyqxmd.com/read/27852706/efficacy-of-two-injection-site-localisation-techniques-for-botulinum-toxin-injections-a-single-blind-crossover-randomised-trial-protocol-among-adults-with-hemiplegia-due-to-stroke
#6
Claire Morel, Isabelle Hauret, Nicolas Andant, Armand Bonnin, Bruno Pereira, Emmanuel Coudeyre
INTRODUCTION: Botulinum toxin injections are an effective treatment for limb spasticity following stroke. Different tracking techniques are used for this purpose: palpation, electrostimulation, electromyography and ultrasound. Yet very few studies have compared these different techniques, and none has successfully proved the superior efficacy of ultrasound-guided injections compared to another tracking method. The primary objective of our study was therefore to compare the efficacy of botulinum toxin injections depending on the tracking technique used: ultrasound versus electrostimulation...
November 15, 2016: BMJ Open
https://www.readbyqxmd.com/read/27845663/demonstration-of-2-mm-thick-microcontrolled-injectable-stimulators-based-on-rectification-of-high-frequency-current-bursts
#7
Laura Becerra Fajardo, Marieluise Schmidbauer, Antoni Ivorra
Existing implantable stimulators use powering approaches that result in stiff and bulky systems or result in systems incapable of producing the current magnitudes required for neuromuscular stimulation. This hampers their use in neuroprostheses for paralysis. We previously demonstrated an electrical stimulation method based on electronic rectification of high frequency (HF) current bursts. The implants act as rectifiers of HF current that flows through the tissues by galvanic coupling, transforming this current into low frequency current capable of performing neuromuscular stimulation...
October 31, 2016: IEEE Transactions on Neural Systems and Rehabilitation Engineering
https://www.readbyqxmd.com/read/27843073/off-target-effect-of-sildenafil-on-postsurgical-erectile-dysfunction-alternate-pathways-and-localized-delivery-system
#8
Amirali Salmasi, Geun Taek Lee, Neal Patel, Ritu Goyal, Michael Dinizo, Young Suk Kwon, Part K Modi, Izak Faiena, Hee-Jin Kim, Nara Lee, Johanna L Hannan, Joachim Kohn, Isaac Yi Kim
INTRODUCTION: There is no consensus on the best oral phosphodiesterase type 5 inhibitor (PDE5I) for patients undergoing penile rehabilitation after surgical nerve injury. AIM: To determine the mechanism of PDE5I on cultured neuronal cells and the effectiveness of local drug delivery using nanospheres (NSPs) to sites of nerve injury in a rat model of bilateral cavernous nerve injury (BCNI). METHODS: The effects of sildenafil, tadalafil, and vardenafil on cyclic adenosine monophosphate, cyclic guanosine monophosphate, and cell survival after exposure to hypoxia and H2O2 were measured in PC12, SH-SY5Y, and NTERA-2 (NT2) cell cultures...
December 2016: Journal of Sexual Medicine
https://www.readbyqxmd.com/read/27831886/demonstration-of-2-mm-thick-microcontrolled-injectable-stimulators-based-on-rectification-of-high-frequency-current-bursts
#9
Laura Becerra-Fajardo, Marieluise Schmidbauer, Antoni Ivorra
Existing implantable stimulators use powering approaches that result in stiff and bulky systems or result in systems incapable of producing the current magnitudes required for neuromuscular stimulation. This hampers their use in neuroprostheses for paralysis. We previously demonstrated an electrical stimulation method based on electronic rectification of high frequency (HF) current bursts. The implants act as rectifiers of HF current that flows through the tissues by galvanic coupling, transforming this current into low frequency current capable of performing neuromuscular stimulation...
October 31, 2016: IEEE Transactions on Neural Systems and Rehabilitation Engineering
https://www.readbyqxmd.com/read/27793063/electrostimulation-training-effects-on-diurnal-fluctuations-of-neuromuscular-performance
#10
H Gueldich, N Zarrouk, H Chtourou, F Zghal, S Sahli, H Rebai
This study examined the effects of electrostimulation (ES) strength training at the same time-of-day on the diurnal fluctuations of maximal voluntary isometric contractions (MVIC) and surface electromyography (EMG). 20 male performed 3 MVICs of knee extension coupled with surface EMG before and after 5 weeks of 3 ES training sessions per week. Each ES training session consisted in 45 isometric contractions. The participants were randomly assigned to either a morning (MTG, 07:00-08:00 h) or an evening (ETG, 17:00-18:00 h) training group...
October 28, 2016: International Journal of Sports Medicine
https://www.readbyqxmd.com/read/27757354/the-lesser-palatine-nerve-innervates-the-levator-veli-palatini-muscle
#11
Hideaki Kishimoto, Yoshitaka Matsuura, Katsuya Kawai, Shigehito Yamada, Shigehiko Suzuki
When the lesser palatine nerve (LPN) is supposed to be a branch of the trigeminal nerve and innervate sensation of the soft palate, whether the LPN contains motor fibers is unclear. In this study, we monitored the electromyogram of the levator veli palatini (LVP) muscle on stimulating the LPN during palatoplasty in 3 patients. The electromyogram of the muscles showed the myogenic potential induced by electrostimulation of the LPN. Taken together with the finding from our previous anatomical study that the motor fibers come from the facial nerve, this result supports the double innervation theory of the LVP, which posits that both the pharyngeal plexus and the facial nerve innervate it...
September 2016: Plastic and Reconstructive Surgery. Global Open
https://www.readbyqxmd.com/read/27756671/intraoperative-subcortical-electrical-mapping-of-the-optic-tract-in-awake-surgery-using-virtual-reality-headset
#12
Edouard Mazerand, Marc Le Renard, Sophie Hue, Jean-Michel Lemee, Evelyne Klinger, Philippe Menei
BACKGROUND: Brain mapping during awake craniotomy is a well-known technique to preserve neurological functions, especially the language. It is still challenging to map the optic radiations due to the difficulty to test the visual field intraoperatively. OBJECTIVE: To assess the visual field during awake craniotomy, we developed the Functions' Explorer based on a Virtual Reality Headset (FEX-VRH). METHODS: The impaired visual field of ten patients was tested with the gold standard exam (an automated perimetry), and the FEX-VRH...
October 15, 2016: World Neurosurgery
https://www.readbyqxmd.com/read/27756010/electrotonic-potentials-in-aloe-vera-l-effects-of-intercellular-and-external-electrodes-arrangement
#13
Alexander G Volkov, Eunice K Nyasani, Clayton Tuckett, Jessenia M Scott, Mariah M Z Jackson, Esther A Greeman, Ariane S Greenidge, Devin O Cohen, Maia I Volkova, Yuri B Shtessel
Electrostimulation of plants can induce plant movements, activation of ion channels, ion transport, gene expression, enzymatic systems activation, electrical signaling, plant-cell damage, enhanced wound healing, and influence plant growth. Here we found that electrical networks in plant tissues have electrical differentiators. The amplitude of electrical responses decreases along a leaf and increases by decreasing the distance between polarizing Pt-electrodes. Intercellular Ag/AgCl electrodes inserted in a leaf and extracellular Ag/AgCl electrodes attached to the leaf surface were used to detect the electrotonic potential propagation along a leaf of Aloe vera...
October 12, 2016: Bioelectrochemistry
https://www.readbyqxmd.com/read/27753086/high-variability-of-facial-muscle-innervation-by-facial-nerve-branches-a-prospective-electrostimulation-study
#14
Ashraf Raslan, Gerd Fabian Volk, Martin Möller, Vincent Stark, Nikolas Eckhardt, Orlando Guntinas-Lichius
OBJECTIVES/HYPOTHESIS: To examine by intraoperative electric stimulation which peripheral facial nerve (FN) branches are functionally connected to which facial muscle functions. STUDY DESIGN: Single-center prospective clinical study. METHODS: Seven patients whose peripheral FN branching was exposed during parotidectomy under FN monitoring received a systematic electrostimulation of each branch starting with 0.1 mA and stepwise increase to 2 mA with a frequency of 3 Hz...
October 18, 2016: Laryngoscope
https://www.readbyqxmd.com/read/27734816/-effects-of-electrostimulation-therapy-in-selected-cases-of-vocal-fold-paralysis
#15
Bożena Kosztyła-Hojna, Anna Kraszewska, Anna Łobaczuk-Sitnik, Diana Moskal, Dawid Falkowski, Jolanta Biszewska, Greta Berger
: Paralytic dysphonia is the most serious neurogenic pathology of voice quality. An important issue is to identify methods which support routine treatment. AIM: The aim of the study is to assess voice quality after electrostimulation (ES) therapy in patients with paralytic dysphonia. MATERIALS AND METHODS: The group consisted of 40 patients diagnosed at the Department of Clinical Phonoaudiology and Logopedics and then treated at the Phoniatric Outpatient Clinic of the Medical University of Bialystok, Poland in years 2013-2015...
July 29, 2016: Polski Merkuriusz Lekarski: Organ Polskiego Towarzystwa Lekarskiego
https://www.readbyqxmd.com/read/27683070/analysis-of-retinal-function-using-chromatic-pupillography-in-retinitis-pigmentosa-and-the-relationship-to-electrically-evoked-phosphene-thresholds
#16
Carina Kelbsch, Fumiatsu Maeda, Jolanta Lisowska, Lukasz Lisowski, Torsten Strasser, Krunoslav Stingl, Barbara Wilhelm, Helmut Wilhelm, Tobias Peters
PURPOSE: To analyse pupil responses to specific chromatic stimuli in patients with advanced retinitis pigmentosa (RP) to ascertain whether chromatic pupillography can be used as an objective marker for residual retinal function. To examine correlations between parameters of the pupil response and the perception threshold of electrically evoked phosphenes. METHODS: Chromatic pupillography was performed in 40 patients with advanced RP (visual acuity < 0.02 or visual field ≤5°, non-recordable ERGs) and 40 age-matched healthy subjects...
September 29, 2016: Acta Ophthalmologica
https://www.readbyqxmd.com/read/27678468/reduced-corporal-fibrosis-to-protect-erectile-function-by-inhibiting-the-rho-kinase-lim-kinase-cofilin-pathway-in-the-aged-transgenic-rat-harboring-human-tissue-kallikrein-1
#17
Kai Cui, Yang Luan, Tao Wang, Li Zhuan, Ke Rao, Shao-Gang Wang, Zhang-Qun Ye, Ji-Hong Liu, Dao-Wen Wang
Our previous studies have demonstrated that erectile function was preserved in aged transgenic rats (TGR) harboring the human tissue kallikrein 1 (hKLK1), while the molecular level of hKLK1 on corporal fibrosis to inhibit age-related erectile dysfunction (ED) is poorly understood. Male wild-type Sprague-Dawley rats (WTR) and TGR harboring the hKLK1 gene were fed to 4- or 18-month-old and divided into three groups: young WTR (yWTR) as the control, aged WTR (aWTR), and aged TGR (aTGR). Erectile function of all rats was assessed by cavernous nerve electrostimulation method...
September 27, 2016: Asian Journal of Andrology
https://www.readbyqxmd.com/read/27676798/ultrasound-guided-injection-of-botulinum-toxin-in-upper-limb-experience-of-pmr-departement
#18
Samira Lahrabli, Herman Azanmasso, Fatima Lmidmani, Abdellatif El Fatimi
OBJECTIVE: Spasticity of the upper limb is a disabling symptom in patients with vascular hemiplegia, both functionally and orthopedic evolution of these patients. Many treatments exist for spasticity, and botulinum toxin may be an attractive therapeutic alternative, to fight against the often deleterious spasticity. MATERIAL/PATIENTS AND METHODS: We report a retrospective work of 10 spastic patients treated with ultrasound-guided injection of botulinum toxin into the MPR Casablanca service...
September 2016: Annals of Physical and Rehabilitation Medicine
https://www.readbyqxmd.com/read/27664964/balancing-the-2-hemispheres-in-simple-calculation-evidence-from-direct-cortical-electrostimulation
#19
C Semenza, E Salillas, S De Pallegrin, A Della Puppa
How do the parietal lobes contribute to simple calculation? Clinical and neuroimaging methods, which are based mainly on correlational evidence, have provided contrasting results so far. Here we used direct cortical electrostimulation during brain surgery to causally infer the role of the left and right parietal lobes in simple calculation. Stimulation provoked errors for addition and multiplication in different parietal areas on both hemispheres. Crucially, an innovative qualitative error analysis unveiled the functional contrast of the 2 parietal lobes...
September 24, 2016: Cerebral Cortex
https://www.readbyqxmd.com/read/27660329/transcutaneous-periorbital-electrical-stimulation-in-the-treatment-of-dry-eye
#20
Emilio Pedrotti, Francesca Bosello, Adriano Fasolo, Anna C Frigo, Ivan Marchesoni, Alfredo Ruggeri, Giorgio Marchini
PURPOSE: To evaluate efficacy and safety of transcutaneous application of electrical current on symptoms and clinical signs of dry eye (DE). METHODS: 27 patients with DE underwent transcutaneous electrostimulation with electrodes placed onto the periorbital region of both eyes and manual stimulation with a hand-piece conductor moved by the operator. Each patient underwent 12 sessions of 22 min spread over 2 months, two sessions per week in the first month and one session per week in the second month...
September 22, 2016: British Journal of Ophthalmology
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