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phosphatase signalling

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https://www.readbyqxmd.com/read/29341308/stiffness-regulates-the-proliferation-and-osteogenic-odontogenic-differentiation-of-human-dental-pulp-stem-cells-via-the-wnt-signalling-pathway
#1
Nanxin Liu, Mi Zhou, Qi Zhang, Tao Zhang, Taoran Tian, Quanquan Ma, Changyue Xue, Shiyu Lin, Xiaoxiao Cai
OBJECTIVES: Researches showed that stiffness of the extracellular matrix can affect the differentiation of many stem cells. Dental pulp stem cells (DPSCs) are a promising type of adult stem cell. However, we know little about whether and how the behaviour of DPSCs is influenced by stiffness. MATERIALS AND METHODS: We carried out a study that cultured DPSCs on tunable elasticity polydimethylsiloxane substrates to investigate the influence on morphology, proliferation, osteogenic/odontogenic differentiation and its possible mechanism...
January 17, 2018: Cell Proliferation
https://www.readbyqxmd.com/read/29339153/inhibition-of-cox-2-and-5-lox-regulates-the-progression-of-colorectal-cancer-by-promoting-pten-and-suppressing-pi3k-akt-pathway
#2
Jian Chang, Nan Tang, Qi Fang, Kongfan Zhu, Lei Liu, Xingcheng Xiong, Zhongchao Zhu, Bixiang Zhang, Mingzhi Zhang, Jing Tao
For colorectal cancer (CRC) patients, local and systemic inflammatory responses have been extensively reported to closely associate with patient survival. However, the specific signalling pathways responsible for carcinogenic responses are unclear. Phosphatase and tensin homolog deleted on chromosome 10 (PTEN) is a negative regulator of PI3K/AKT pathway that is gradually inactivated in cancers through mutation, loss of heterozygosity and others epigenetic mechanisms. In addition, COX and LOX metabolic pathways of arachidonic acid (AA) play a crucial role in promoting adenoma development...
January 12, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29338103/interrogating-protein-phosphatases-with-chemical-activity-probes
#3
Garrett Casey, Cliff Stains
Protein phosphatases, while long overlooked, have recently become appreciated as drivers of both normal- and disease-associated signaling events. As a result, the spotlight is now turning torwards this enzyme family and efforts geared towards the development of modern chemical tools for studying these enzymes are well underway. This minireview focuses on the evolution of chemical activity probes, both optical and covalent, for the study of protein phosphatases. Small-molecule probes, global monitoring of phosphatase activity through the use of covalent modifiers, and targeted fluorescence-based activity probes are discussed...
January 16, 2018: Chemistry: a European Journal
https://www.readbyqxmd.com/read/29338035/genome-wide-identification-of-wheat-and-brachypodium-type-one-protein-phosphatases-and-functional-characterization-of-durum-wheat-tdpp1a
#4
Mariem Bradai, Habib Mahjoubi, Andrea Chini, Marie-Edith Chabouté, Moez Hanin, Chantal Ebel
Reversible phosphorylation is an essential mechanism regulating signal transduction during development and environmental stress responses. An important number of dephosphorylation events in the cell are catalyzed by type one protein phosphatases (PP1), which catalytic activity is driven by the binding of regulatory proteins that control their substrate specificity or subcellular localization. Plants harbor several PP1 isoforms accounting for large functional redundancies. While animal PP1s were reported to play relevant roles in controlling multiple cellular processes, plant orthologs remain poorly studied...
2018: PloS One
https://www.readbyqxmd.com/read/29337188/abrogation-of-fam20c-altered-cell-behaviors-and-bmp-signaling-of-immortalized-dental-mesenchymal-cells
#5
Chao Liu, Nan Zhou, Yu Wang, Hua Zhang, Priyam Jani, Xiaofang Wang, Yongbo Lu, Nan Li, Jing Xiao, Chunlin Qin
FAM20C mutations compromise the mineralization of skeleton and tooth in both human and mouse. Putatively, the mineralization disorder is attributed to the elevated fibroblast growth factor 23 (FGF23), which reduced the serum phosphorus by suppressing the reabsorption of phosphorus in kidney. Besides the regulation on systemic phosphorus homeostasis, FAM20C was also implicated to regulate cell behaviors and gene expression through a cell-autonomous manner. To identify the primary effects of Fam20c on dental mesenchymal cells, mouse Fam20c-deficient dental mesenchymal cells were generated by removing the floxed alleles from the immortalized mouse Fam20cf/f dental mesenchymal cells with Cre-expressing lentivirus...
January 11, 2018: Experimental Cell Research
https://www.readbyqxmd.com/read/29336472/baicalin-increases-hair-follicle-development-by-increasing-canonical-wnt-%C3%AE-%C3%A2-catenin-signaling-and-activating-dermal-papillar-cells-in-mice
#6
Fei Xing, Wen-Juan Yi, Fang Miao, Meng-Yun Su, Tie-Chi Lei
Baicalin is a traditional Chinese herbal medicine commonly used for hair loss, the precise molecular mechanism of which is unknown. In the present study, the mechanism of baicalin was investigated via the topical application of baicalin to reconstituted hair follicles on mice dorsa and evaluating the effect on canonical Wnt/β‑catenin signaling in the hair follicles and the activity of dermal papillar cells. The results indicate that baicalin stimulates the expression of Wnt3a, Wnt5a, frizzled 7 and disheveled 2 whilst inhibiting the Axin/casein kinase 1α/adenomatous polyposis coli/glycogen synthase kinase 3β degradation complex, leading to accumulation of β‑catenin and activation of Wnt/β‑catenin signaling...
January 16, 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29335436/deregulated-pp1%C3%AE-phosphatase-activity-towards-mapk-activation-is-antagonized-by-a-tumor-suppressive-failsafe-mechanism
#7
Ming Chen, Lixin Wan, Jiangwen Zhang, Jinfang Zhang, Lourdes Mendez, John G Clohessy, Kelsey Berry, Joshua Victor, Qing Yin, Yuan Zhu, Wenyi Wei, Pier Paolo Pandolfi
The mitogen-activated protein kinase (MAPK) pathway is frequently aberrantly activated in advanced cancers, including metastatic prostate cancer (CaP). However, activating mutations or gene rearrangements among MAPK signaling components, such as Ras and Raf, are not always observed in cancers with hyperactivated MAPK. The mechanisms underlying MAPK activation in these cancers remain largely elusive. Here we discover that genomic amplification of the PPP1CA gene is highly enriched in metastatic human CaP. We further identify an S6K/PP1α/B-Raf signaling pathway leading to activation of MAPK signaling that is antagonized by the PML tumor suppressor...
January 15, 2018: Nature Communications
https://www.readbyqxmd.com/read/29335246/differential-roles-of-errfi1-in-egfr-and-akt-pathway-regulation-affect-cancer-proliferation
#8
Junmei Cairns, Brooke L Fridley, Gregory D Jenkins, Yongxian Zhuang, Jia Yu, Liewei Wang
AKT signaling is modulated by a complex network of regulatory proteins and is commonly deregulated in cancer. Here, we present a dual mechanism of AKT regulation by the ERBB receptor feedback inhibitor 1 (ERRFI1). We show that in cells expressing high levels of EGFR, ERRF1 inhibits growth and enhances responses to chemotherapy. This is mediated in part through the negative regulation of AKT signaling by direct ERRFI1-dependent inhibition of EGFR In cells expressing low levels of EGFR, ERRFI1 positively modulates AKT signaling by interfering with the interaction of the inactivating phosphatase PHLPP with AKT, thereby promoting cell growth and chemotherapy desensitization...
January 15, 2018: EMBO Reports
https://www.readbyqxmd.com/read/29334873/nobiletin-prevents-cadmium-induced-neuronal-apoptosis-by-inhibiting-reactive-oxygen-species-and-modulating-jnk-erk1-2-and-akt-mtor-networks-in-rats
#9
Youyang Qu, Yu Liu, Li Chen, Yanmei Zhu, Xingjun Xiao, Di Wang, Yulan Zhu
Objectives Cadmium (Cd), an extremely noxious environmental pollutant is known to induce oxidative stress leading to neurodegenerative diseases. Nobiletin, a citrus flavonoid is reported to possess various pharmacological properties. This study investigates the effects of nobiletin over Cd-induced neuronal apoptosis in rodent experimental model. Methods To separate group of male Sprague Dawley rats, Cd (2 mL/kg/day) was subcutaneously injected for one month which results in a dose level of 1 mg/kg Cd. Couple of days prior to Cd injection, the treatment group rats regularly received nobiletin (50, 100, or 200 mg/kg b...
January 16, 2018: Neurological Research
https://www.readbyqxmd.com/read/29334773/macf1-overexpression-by-transfecting-the-21-kbp-large-plasmid-pegfp-c1a-acf7-promotes-osteoblast-differentiation-and-bone-formation
#10
Yan Zhang, Chong Yin, Lifang Hu, Zhihao Chen, Fan Zhao, Dijie Li, Jianhua Ma, Xiaoli Ma, Peihong Su, Wuxia Qiu, Chaofei Yang, Pai Wang, Siyu Li, Ge Zhang, Liping Wang, Airong Qian, Cory Xian
Microtubule actin crosslinking factor 1 (MACF1) is a large spectraplakin protein known to have crucial roles in regulating cytoskeletal dynamics, cell migration, growth and differentiation. However, its role and action mechanism in bone remain unclear. This study investigated the optimal conditions for effective transfection of the large plasmid PEGFP-C1A-ACF7 (~21 kbp) containing the full-length human MACF1 cDNA and the potential role of MACF1 in bone formation. To enhance MACF1 expression, the large plasmid was transfected into osteogenic cells by electroporation in vitro and mouse calvaria with nanoparticles...
January 15, 2018: Human Gene Therapy
https://www.readbyqxmd.com/read/29331981/variant-in-c-terminal-region-of-intestinal-alkaline-phosphatase-associated-with-benign-familial-hyperphosphatasaemia
#11
Takayuki Ishige, Sakae Itoga, Emi Utsuno, Motoi Nishimura, Masaharu Yoshikawa, Naoya Kato, Kazuyuki Matsushita, Osamu Yokosuka, Fumio Nomura
BACKGROUND: A genetic diagnosis has been rarely performed in benign familial hyperphosphatasaemia, and molecular mechanism largely remains unclear. OBJECTIVES: We encountered a case with benign familial hyperphosphatasaemia of intestinal alkaline phosphatase (IAP). To elucidate the molecular mechanism, we performed ALPI gene sequencing and in vitro protein expression analysis. METHODS: ALPI gene was sequenced by long-range PCR and massively parallel sequencing...
January 13, 2018: Journal of Medical Genetics
https://www.readbyqxmd.com/read/29330357/knockdown-of-phospholipase-c%C3%AE%C2%B5-plc%C3%AE%C2%B5-inhibits-cell-proliferation-via-phosphatase-and-tensin-homolog-deleted-on-chromosome-10-pten-akt-signaling-pathway-in-human-prostate-cancer
#12
Xiao Wang, Yanru Fan, Zhongbo Du, Jiaxin Fan, Yanni Hao, Jinhua Wang, Xiaohou Wu, Chunli Luo
BACKGROUND Phospholipase Cε (PLCε), a member of the plc family, has been extensively studied to reveal its role in the regulation of different cell functions, but understanding of the underlying mechanisms remains limited. In the present study, we explored the effects of PLCε on PTEN (phosphatase and tensin homolog deleted on chromosome 10) in cell proliferation in prostate cancer cells. MATERIAL AND METHODS We assessed PLCε and PTEN expression in human benign prostate tissues compared to prostate cancer tissues by immunohistochemistry...
January 13, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29330298/stemness-is-enhanced-in-gastric-cancer-by-a-set-pp2a-e2f1-axis
#13
Shuhei Enjoji, Ryotaro Yabe, Shunya Tsuji, Kazuhiro Yoshimura, Hideyoshi Kawasaki, Masashi Sakurai, Yusuke Sakai, Hiroko Takenouchi, Shigefumi Yoshino, Shoichi Hazama, Hiroaki Nagano, Hiroko Oshima, Masanobu Oshima, Michael P Vitek, Tetsuya Matsuura, Yoshitaka Hippo, Tatsuya Usui, Takashi Ohama, Koichi Sato
Gastric cancer is the fifth most common malignancy and the third leading cause of cancer-related deaths worldwide. Chemotherapies against gastric cancer often fail, with cancer recurrence due potentially to the persistence of cancer stem cells. This unique subpopulation of cells in tumors possess the ability to self-renew and de-differentiate. These cancer stem cells are critical for initiation, maintenance, metastasis, and relapse of cancers; however, the molecular mechanisms supporting cancer stemness remain largely unknown...
January 12, 2018: Molecular Cancer Research: MCR
https://www.readbyqxmd.com/read/29329711/colorimetric-logic-gate-for-alkaline-phosphatase-based-on-copper-ii-based-metal-organic-frameworks-with-peroxidase-like-activity
#14
Caihong Wang, Jie Gao, Yili Cao, Hongliang Tan
Nanozymes have been extensively exploited in the construction of colorimetric sensors in the past decade. However, as lack of specific recognition element, these sensors are mostly limited in the detection of hydrogen peroxide and its related agents. In this work, a colorimetric assay for the detection of alkaline phosphatase (ALP) activity was developed by employing copper (II)-based metal-organic frameworks (Cu-MOFs) as peroxidase mimic and pyrophosphate (PPi) as recognition element. Benefiting from its intrinsic porosity, Cu-MOFs built from one-step self-assembly showed a superior catalytic activity over horseradish peroxidase...
April 3, 2018: Analytica Chimica Acta
https://www.readbyqxmd.com/read/29328971/ultrasensitive-electrochemical-sensing-of-hg2-based-on-thymine-hg2-thymine-interaction-and-signal-amplification-of-alkaline-phosphatase-catalyzed-silver-deposition
#15
Aigui Xu, Long Chao, Hongbo Xiao, Yuyun Sui, Jia Liu, Qingji Xie, Shouzhuo Yao
We report an ultrasensitive electrochemical sensor for Hg2+ detection, on the basis of two Hg2+-specific oligonucleotide probes (a thiolated capture probe and a biotinated signal probe), "terminal" signal amplification of alkaline phosphatase catalyzed deposition of silver and in situ microliter-droplet anodic stripping voltammetry (ASV). The thiolated capture probe is immobilized on an Au-plated glassy carbon electrode, and the biotinated signal probe is then attached to the electrode surface through the thymine-Hg2+-thymine interaction in the presence of Hg2+...
January 5, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29327280/comparison-of-the-ding-protein-from-the-archaeon-sulfolobus-solfataricus-with-human-phosphate-binding-protein-and-pseudomonas-fluorescence-ding-counterparts
#16
Elena Porzio, Anna De Maio, Teresa Ricciardi, Carmela Mistretta, Giuseppe Manco, Maria Rosaria Faraone-Mennella
DING proteins represent a new group of 40 kDa-related members, ubiquitous in living organisms. The family also include the DING protein from Sulfolobus solfataricus, functionally related to poly(ADP-ribose) polymerases. Here, the archaeal protein has been compared with the human Phosphate-Binding Protein and the Pseudomonas fluorescence DING enzyme, by enzyme assays and immune cross-reactivity. Surprisingly, as the Sulfolobus enzyme, the Human and Pseudomonas proteins display poly(ADP-ribose) polymerase activity, whereas a phosphatase activity was only present in Sulfolobus and human protein, despite the conserved phosphate-binding site residues in Pseudomonas DING...
January 11, 2018: Extremophiles: Life Under Extreme Conditions
https://www.readbyqxmd.com/read/29326975/identifying-the-substrate-proteins-of-u-box-e3s-e4b-and-chip-by-orthogonal-ubiquitin-transfer
#17
Karan Bhuripanyo, Yiyang Wang, Xianpeng Liu, Li Zhou, Ruochuan Liu, Duc Duong, Bo Zhao, Yingtao Bi, Han Zhou, Geng Chen, Nicholas T Seyfried, Walter J Chazin, Hiroaki Kiyokawa, Jun Yin
E3 ubiquitin (UB) ligases E4B and carboxyl terminus of Hsc70-interacting protein (CHIP) use a common U-box motif to transfer UB from E1 and E2 enzymes to their substrate proteins and regulate diverse cellular processes. To profile their ubiquitination targets in the cell, we used phage display to engineer E2-E4B and E2-CHIP pairs that were free of cross-reactivity with the native UB transfer cascades. We then used the engineered E2-E3 pairs to construct "orthogonal UB transfer (OUT)" cascades so that a mutant UB (xUB) could be exclusively used by the engineered E4B or CHIP to label their substrate proteins...
January 2018: Science Advances
https://www.readbyqxmd.com/read/29323748/the-gain-of-function-mutation-e76k-in-shp2-promotes-cac-tumorigenesis-and-induces-emt-via-the-wnt-%C3%AE-catenin-signaling-pathway
#18
Qian Zhang, Rongrong Zhao, Chuling Fan, Xinyi Wang, Youzhi Xu, Yakun Liu, Siying Wang
SHP2 is encoded by the protein tyrosine phosphatase 11 (Ptpn11) gene. Several gain-of-function (GOF) mutations in Ptpn11 have been identified in human hematopoietic malignancies and solid tumors. In addition, the mutation rate for SHP2 is the highest for colorectal cancer (CRC) among solid tumors. The E76K GOF mutation is the most common and active SHP2 mutation; however, the pathogenic effects and function of this mutation in CRC tumor progression have not been well characterized. The Wnt/β-catenin (CTNNB1) signaling pathway is crucial for CRC, and excessive activation of this pathway has been observed in several tumors...
January 11, 2018: Molecular Carcinogenesis
https://www.readbyqxmd.com/read/29323740/long-non-coding-rna-asbel-promotes-osteosarcoma-cell-proliferation-migration-and-invasion-by-regulating-microrna-21
#19
Jianhui Zhao, Zhongli Gao, Chao Zhang, Han Wu, Rui Gu, Rui Jiang
Osteosarcoma is the most common malignant bone tumor in children and adolescents with high rate of incidence, high frequency of recurrence and high degree of metastasis. This study aimed to investigate the effects of long noncoding RNA antisense ncRNA in the Abundant in neuroepithelium area (ANA)/B-cell translocation gene 3 (BTG3) locus (lncRNA ASBEL) on the pathogenesis of osteosarcoma. The expression levels of ASBEL in human osteoblast cells and human osteosarcoma cells were evaluated using qRT-PCR. Effects of ASBEL knockdown on cell viability, migration and invasion were detected using trypan blue exclusion assay, cell migration and invasion assay, respectively...
January 11, 2018: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29320690/sharing-of-phosphatases-promotes-response-plasticity-in-phosphorylation-cascades
#20
Bhaswar Ghosh, Uddipan Sarma, Victor Sourjik, Stefan Legewie
Sharing of positive or negative regulators between multiple targets is frequently observed in cellular signaling cascades. For instance, phosphatase sharing between multiple kinases is ubiquitous within the MAPK pathway. Here we investigate how such phosphatase sharing could shape robustness and evolvability of the phosphorylation cascade. Through modeling and evolutionary simulations, we demonstrate that 1) phosphatase sharing dramatically increases robustness of a bistable MAPK response, and 2) phosphatase-sharing cascades evolve faster than nonsharing cascades...
January 9, 2018: Biophysical Journal
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