Harley Robinson, Jayde E Ruelcke, Amanda Lewis, Charles S Bond, Archa H Fox, Vandhana Bharti, Shivangi Wani, Nicole Cloonan, Andrew Lai, David Margolin, Li Li, Carlos Salomon, Renée S Richards, Aine Farrell, Robert A Gardiner, Robert G Parton, Alexandre S Cristino, Michelle M Hill
BACKGROUND: Caveolae proteins play diverse roles in cancer development and progression. In prostate cancer, non-caveolar caveolin-1 (CAV1) promotes metastasis, while CAVIN1 attenuates CAV1-induced metastasis. Here, we unveil a novel mechanism linking CAV1 to selective loading of exosomes with metastasis-promoting microRNAs. RESULTS: We identify hnRNPK as a CAV1-regulated microRNA binding protein. In the absence of CAVIN1, non-caveolar CAV1 drives localisation of hnRPNK to multi-vesicular bodies (MVBs), recruiting AsUGnA motif-containing miRNAs and causing their release within exosomes...
April 2021: Clinical and Translational Medicine