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Eduardo Javier Houghton, Miguel A Morano, Daniel Pedreira, Carlos Balteiro
BACKGROUND: The retained bile duct stones with distant fistulous tract has been treated by the transfistular route as the first line of treatment since the publications of Dr Mazzariello. Several techniques have been developed and several studies have been published, but little has been assessed about the relationship between complications and differences in extraction techniques. OBJECTIVE: This study aimed to determine the percentage of therapeutic success and determine which variables influence the occurrence of complications...
June 2014: Acta Gastroenterologica Latinoamericana
M Dip, E Halac, G Cervio, L Rojas, G Bianco, O Imventarza, S Rodríguez Bruno
INTRODUCTION: The need to measure the level of surgical risk arises from its direct relationship with the development of postoperative complications and the use of hospital resources. The construction of a surgical risk scale (ERQ) for pediatric surgery should be based on a common language, by using a small number of easy to collect and reproduce variables. The objectives of this study were to construct an ERQ for pediatric patients and to analyze the surgical performance. MATERIALS AND METHODS: We retrospectively analyzed 105 primary surgical procedures under general anesthesia performed by liver transplantation service at Hospital Garrahan, between 29/6/08 and 25/3/10...
January 2011: Cirugía Pediátrica: Organo Oficial de la Sociedad Española de Cirugía Pediátrica
Kun Zhou, Cai-Hong Hu, Li-Fang Huang, Wen-Li Liu, Han-Ying Sun
OBJECTIVE: To explore the mechanism of Wnt and Notch pathway involved modulating time and spatial restricted hematopoiesis. METHODS: Murine hematopoietic stem and progenitor cells (HSPCs) were isolated from bone marrow (BM) by using c-kit microbeads. E10.5 aorta-gonad-mesonephros (AGM), E12.5, E14.5, E16.5 fetal liver (FL) and adult BM derived stromal cells (StroCs) were isolated and co-cultured with c-kit(+)HSPCs. The floating cells in co-culture system were sorted and counted by FACS...
December 2009: Zhonghua Xue Ye Xue za Zhi, Zhonghua Xueyexue Zazhi
Kun Zhou, Caihong Hu, Zhigang Zhou, Lifang Huang, Wenli Liu, Hanying Sun
Future application of hematopoietic stem and progenitor cells (HSPCs) in clinical therapies largely depends on their successful expansion in vitro. Fetal liver (FL) is a unique hematopoietic organ in which hematopoietic cells markedly expand in number, but the mechanisms involved remain unclear. Stromal cells (StroCs) have been suggested to provide a suitable cellular environment for in vitro expansion of HSPCs. In this study, murine StroCs derived from FL at E14.5, with a high level of Sonic hedgehog (Shh) and Wnt expression, were found to have an increased ability to support the proliferation of HSPCs...
September 25, 2009: Biochemical and Biophysical Research Communications
Caroline Lemieux, Isabelle Cloutier, Jean-François Tanguay
The recent interest in the role of bone marrow (BM)-derived endothelial progenitor cells (EPCs) and the benefits of estrogen on cardiovascular health brought us to evaluate if estrogen could affect cardiac repair more broadly by regulating biological processes involved in the functional organization of the BM stem cell (SC) niche. To assess such possibility, we evaluated gene expression profiles of BM c-kit+ SCs and CD44+ stromal cells (StroCs) after exposure to a physiological concentration of 17beta-estradiol (17betaE)...
December 2008: Stem Cells and Development
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