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https://www.readbyqxmd.com/read/29463416/irradiation-of-rainbow-trout-at-early-life-stages-results-in-a-proteomic-legacy-in-adult-gills-part-a-proteomic-responses-in-the-irradiated-fish-and-in-non-irradiated-bystander-fish
#1
Richard W Smith, Richard D Moccia, Colin B Seymour, Carmel E Mothersill
Exposure to a single 0.5 Gy X-ray dose of eggs at 48 h after fertilisation (48 h egg), eyed eggs, yolk sac larvae (YSL) and first feeders induces a legacy effect in adult rainbow trout. This includes the transmission of a bystander effect to non-irradiated adult trout which had swam with the irradiated fish. The aim of this study was to investigate this legacy by analysing the gill proteome of these irradiated and bystander fish. Irradiation at all of the early life stages resulted in changes to proteins which play a key role in development but are also known to be anti-tumorigenic and anti-oxidant: upregulation of haemoglobin subunit beta (48 h egg), haemoglobin, serum albumin 1 precursor (eyed eggs), clathrin heavy chain 1 isoform X10 (eyed eggs and first feeders), and actin-related protein 2/3 complex subunit 4 (first feeders), downregulation of pyruvate dehydrogenase, histone 1 (48 h egg), triosephosphate isomerase (TPI), collagen alpha-1(1) chain like proteins (YSL), pyruvate kinase PKM-like protein (YSL and first feeders), ubiquitin-40S ribosomal proteins S27 and eukaryotic translation initiation factor 4 A isoform 1B (first feeders)...
February 17, 2018: Environmental Research
https://www.readbyqxmd.com/read/29460293/effect-of-the-sexual-abstinence-period-recommended-by-the-world-health-organization-on-clinical-outcomes-of-fresh-embryo-transfer-cycles-with-normal-ovarian-response-after-intracytoplasmic-sperm-injection
#2
J W Lee, J H Cha, S H Shin, H J Cha, J H Kim, C K Park, K A Pak, J S Yoon, S Y Park
This study was to investigate whether the sexual abstinence period (SAP) recommended by the World Health Organization (WHO) affects clinical outcomes. We compared the rate of clinical outcomes between 2-7 and ≥8 days of SAP in first fresh embryo transfer after intracytoplasmic sperm injection (ICSI) in groups of young maternal age (YMA: <38 years) and old maternal age (OMA: ≥38 years). We conducted a retrospective study of 449 first ICSI cycles with a normal ovarian response. SAP was identified before collecting the semen samples...
February 20, 2018: Andrologia
https://www.readbyqxmd.com/read/29455706/review-the-potential-of-seminal-fluid-mediated-paternal-maternal-communication-to-optimise-pregnancy-success
#3
J J Bromfield
Artificial insemination has been a landmark procedure in improving animal agriculture over the past 150 years. The utility of artificial insemination has facilitated a rapid improvement in animal genetics across agricultural species, leading to improvements of growth, health and productivity in poultry, swine, equine and cattle species. The utility of artificial insemination, as with all assisted reproductive technologies side-steps thousands of years of evolution that has led to the development of physiological systems to ensure the transmission of genetics from generation to generation...
February 19, 2018: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/29453196/genome-wide-association-study-identifies-erbb4-on-2q34-as-a-novel-locus-associated-with-sperm-motility-in-japanese-men
#4
Youichi Sato, Atsushi Tajima, Takehiro Sato, Shiari Nozawa, Miki Yoshiike, Issei Imoto, Aiko Yamauchi, Teruaki Iwamoto
BACKGROUND: The decrease in sperm motility has a potent influence on fertilisation. Sperm motility, represented as the percentage of motile sperm in ejaculated sperms, is influenced by lifestyle habits or environmental factors and by inherited factors. However, genetic factors contributing to individual differences in sperm motility remain unclear. To identify genetic factors that influence human sperm motility, we performed a genome-wide association study (GWAS) of sperm motility. METHODS: A two-stage GWAS was conducted using 811 Japanese men in a discovery stage, followed by a replication study using an additional 779 Japanese men...
February 16, 2018: Journal of Medical Genetics
https://www.readbyqxmd.com/read/29448974/phyto-oestrogens-affect-fertilisation-and-embryo-development-in-vitro-in-sheep
#5
Anna Aryani Amir, Jennifer M Kelly, David O Kleemann, Zoey Durmic, Dominique Blache, Graeme B Martin
Phyto-oestrogens such as isoflavones are natural compounds that can profoundly affect reproductive function. In the present study, we tested whether including isoflavone compounds (genistein, biochanin A, formononetin) in the maturation medium would affect the outcomes for ovine oocytes in vitro. Each isoflavone compound was evaluated at five concentrations (0, 2.5, 5, 10, 25µgmL-1) and the entire protocol was repeated four times. Cumulus-oocyte complexes were randomly allocated to the treatments, then fertilised and cultured in vitro...
February 16, 2018: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/29446833/body-fat-content-fat-distribution-and-adipocytokine-production-and-their-correlation-with-fertility-markers-in-young-adult-men-and-women-conceived-by-icsi
#6
F Belva, J De Schepper, M Roelants, H Tournaye, M Bonduelle, S Provyn
OBJECTIVE: Differences in body fat content during childhood and adolescence have been described in offspring conceived by In Vitro Fertilisation (IVF) and Intracytoplasmic Sperm Injection (ICSI). However, data on body fat and its distribution as well as on adipocytokine production in young adults conceived by ICSI are non-existent. We investigated if young adult men and women conceived by ICSI have a normal body fat patterning and adipocytokine production. DESIGN: Cohort study...
February 15, 2018: Clinical Endocrinology
https://www.readbyqxmd.com/read/29444845/antibacterial-activity-of-male-and-female-sperm-storage-organs-in-ants
#7
Francisco Dávila, Anne Botteaux, David Bauman, Sarah Chérasse, Serge Aron
Bacteria can damage sperm and thus reduce the reproductive success of both males and females; selection should therefore favour the evolution of antimicrobial protection. Eusocial hymenopterans might be particularly affected by such bacterial infections because of their mating ecology. In both sexes, mating is restricted to a short window early in the adult stage; there are no further chances to mate later in life. Males die shortly after mating, but queens use the acquired sperm to fertilise their eggs for years, sometimes decades...
February 14, 2018: Journal of Experimental Biology
https://www.readbyqxmd.com/read/29441594/influence-of-extended-incubation-time-on-human-sperm-chromatin-condensation-sperm-dna-strand-breaks-and-their-effect-on-fertilisation-rate
#8
I Ahmed, S Abdelateef, M Laqqan, H Amor, M A Abdel-Lah, M E Hammadeh
The purpose of this study was to determine influence of extended incubation time on sperm chromatin condensation and DNA strand breaks and their effect on fertilisation rate. Forty couples undergoing ICSI therapy were included. Semen was prepared by PureSperm gradient centrifugation and divided into two parts. The first part (G1) was used immediately for ICSI, whereas the second part (G2) was kept in the incubator at 37°C, 5% and 90% Humidity for 5 hr, and thereafter, the capacitated spermatozoa were used for ICSI...
February 14, 2018: Andrologia
https://www.readbyqxmd.com/read/29440044/acupuncture-and-in-vitro-fertilisation-research-current-and-future-directions
#9
Lee E Hullender Rubin, Belinda J Anderson, LaTasha B Craig
BACKGROUND: Acupuncture is a common adjuvant treatment to support patients undergoing in vitro fertilisation (IVF). However, the impact of acupuncture and the different roles it can play in IVF remain unclear. OBJECTIVE: In this paper, we present an overview and critique of the current evidence on acupuncture's impact on IVF-related stress, describe harms, and propose future directions for investigation. CONCLUSION: Two to three acupuncture sessions performed on or around the day of embryo transfer are insufficient interventions to improve IVF birth outcomes but provide significant IVF-related stress reduction...
February 10, 2018: Acupuncture in Medicine: Journal of the British Medical Acupuncture Society
https://www.readbyqxmd.com/read/29433752/irradiation-of-rainbow-trout-at-early-life-stages-results-in-a-proteomic-legacy-in-adult-gills-part-b-the-effect-of-a-second-radiation-dose-after-one-year-on-the-proteomic-responses-in-the-irradiated-and-non-irradiated-bystander-fish
#10
Richard W Smith, Richard D Moccia, Carmel E Mothersill, Colin B Seymour
This study extends the investigation of the legacy effects of exposure to a single radiation dose at one of four early life stages, in adult rainbow trout (Part A), by examining the effects of a second identical dose after one year; i.e. egg 48 h after fertilisation (48 h egg) + 1 year, eyed egg + 1 year, yolk sac larvae (YSL) + 1 year and first feeder + 1 year. This included the induction of a bystander effect in non-irradiated trout which had swam with the irradiated fish. The second radiation dose negated any beneficial proteomic responses following early life stage irradiation only, particularly irradiation of 48 h eggs and eyed eggs (Part A)...
February 9, 2018: Environmental Research
https://www.readbyqxmd.com/read/29427707/assessment-of-endocrine-disruption-and-oxidative-potential-of-bisphenol-a-triclosan-nonylphenol-diethylhexyl-phthalate-galaxolide-and-carbamazepine-common-contaminants-of-municipal-biosolids
#11
Jo-Anne E Cavanagh, Katherine Trought, Caroline Mitchell, Grant Northcott, Louis A Tremblay
The use of biosolids as a soil conditioner and fertiliser is hindered by the limited knowledge on the risks of micro-contaminants they contain. This study investigated the binding of six organic contaminants commonly found in biosolids, to the estrogen (ER), androgen (AR), aryl hydrocarbon (AhR), and transthyretin (TTR) receptors and their redox activity. Triclosan (TCS), bisphenol-A (BPA), and technical nonylphenol (TNP) had affinity for the TTR with relative potencies of 0.3, 0.03, and 0.076 respectively...
February 7, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29426172/use-of-agricultural-by-products-in-the-development-of-an-agro-energy-chain-a-case-study-from-the-umbria-region
#12
S Venanzi, D Pezzolla, L Cecchini, M Pauselli, A Ricci, A Sordi, B Torquati, G Gigliotti
Use of agricultural and livestock by-products for anaerobic digestion (AD), in total or partial substitution of the maize silage was evaluated from an environmental and economical point of view. The evaluation process included three methodological interdependent and consequential steps: the chemical stage at laboratory and plant level, the environmental and economic steps developing the Life Cycle Assessment and Life Cycle Costing jointly. The laboratory test showed that the two mixtures prepared with by-products, in partial (MIX A) and total (MIX B) substitution of maize silage, did not show differences in bio-methane production compared to a reference mixture with the 33% of maize silage...
January 30, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/29425943/chlorinated-insecticides-toxaphene-and-endrin-affect-oxytocin-testosterone-oestradiol-and-prostaglandin-secretion-from-ovarian-and-uterine-cells-as-well-as-myometrial-contractions-in-cow-in%C3%A2-vitro
#13
Michael Hubert Wrobel, Jaroslaw Mlynarczuk
We examined the direct effects of toxaphene and endrin, chlorinated insecticides that are widespread in the environment, on myometrial contractions and on the secretion of hormones involved in regulating these contractions. Granulosa, luteal, endometrial and myometrial cells, and myometrial strips from non-pregnant cows were incubated with both insecticides at environmentally relevant doses. Toxaphene inhibited and endrin stimulated the secretion of testosterone and oestradiol from granulosa cells. Toxaphene also inhibited and endrin stimulated the expression of the mRNA encoding the precursor of oxytocin (OT), as well OT secretion in luteal cell cultures...
February 3, 2018: Chemosphere
https://www.readbyqxmd.com/read/29411339/evaluation-of-complications-developing-during-and-after-transvaginal-ultrasound-guided-oocyte-retrieval
#14
Selin Özaltın, Serkan Kumbasar, Kadir Savan
OBJECTIVES: To investigate the complications of the oocyte retrieval procedure currently used in in vitro fertilisation. MATERIAL AND METHODS: We examined 1.031 patients who underwent oocyte retrieval in the IVF unit of our hospital for complications developed during and after the procedure. RESULTS: No complications developed related to sedation or general anaesthesia. Vaginal bleeding was observed in 3.1% of the patients. There was no intra-abdominal bleeding or pelvic organ injuries requiring surgery...
2018: Ginekologia Polska
https://www.readbyqxmd.com/read/29408065/closing-the-loop-on-human-urine-plant-availability-of-zeolite-recovered-nutrients-in-a-peat-based-substrate
#15
Siri Caspersen, Zsofia Ganrot
Recycling mineral nutrients from household wastewater is a central step in the development of a circular economy based society. The objective of this study was to evaluate plant availability of mineral elements and plant performance in a peat substrate containing nutrient-enriched zeolite (NEZ) obtained by nutrient recovery from human urine in a source separated wastewater system. Substrate content of potentially available mineral nutrients was determined by CaCl2/DTPA-extraction during a 12 weeks incubation experiment for 20:80 (R20) and 30:70 (R30) volume % of NEZ:sphagnum peat, limed R20 (R20L), and 20:80 vol% of unloaded zeolite:sphagnum peat (Z20)...
February 2, 2018: Journal of Environmental Management
https://www.readbyqxmd.com/read/29390119/metaphase-ii-oocytes-from-human-unilaminar-follicles-grown-in-a-multi-step-culture-system
#16
M McLaughlin, D F Albertini, W H B Wallace, R A Anderson, E E Telfer
STUDY QUESTION: Can complete oocyte development be achieved from human ovarian tissue containing primordial/unilaminar follicles and grown in vitro in a multi-step culture to meiotic maturation demonstrated by the formation of polar bodies and a Metaphase II spindle? SUMMARY ANSWER: Development of human oocytes from primordial/unilaminar stages to resumption of meiosis (Metaphase II) and emission of a polar body was achieved within a serum free multi-step culture system...
January 30, 2018: Molecular Human Reproduction
https://www.readbyqxmd.com/read/29388198/individualised-gonadotropin-dose-selection-using-markers-of-ovarian-reserve-for-women-undergoing-in-vitro-fertilisation-plus-intracytoplasmic-sperm-injection-ivf-icsi
#17
REVIEW
Sarah F Lensen, Jack Wilkinson, Jori A Leijdekkers, Antonio La Marca, Ben Willem J Mol, Jane Marjoribanks, Helen Torrance, Frank J Broekmans
BACKGROUND: During a cycle of in vitro fertilisation plus intracytoplasmic sperm injection (IVF/ICSI), women receive daily doses of gonadotropin follicle-stimulating hormone (FSH) to induce multifollicular development in the ovaries. Generally, the dose of FSH is associated with the number of eggs retrieved. A normal response to stimulation is often considered desirable, for example the retrieval of 5 to 15 oocytes. Both poor and hyper-response are associated with increased chance of cycle cancellation...
February 1, 2018: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/29386627/the-consequences-of-niche-and-physiological-differentiation-of-archaeal-and-bacterial-ammonia-oxidisers-for-nitrous-oxide-emissions
#18
Linda Hink, Cécile Gubry-Rangin, Graeme W Nicol, James I Prosser
High and low rates of ammonium supply are believed to favour ammonia-oxidising bacteria (AOB) and archaea (AOA), respectively. Although their contrasting affinities for ammonium are suggested to account for these differences, the influence of ammonia concentration on AOA and AOB has not been tested under environmental conditions. In addition, while both AOB and AOA contribute to nitrous oxide (N2O) emissions from soil, N2O yields (N2O-N produced per NO2--N generated from ammonia oxidation) of AOA are lower, suggesting lower emissions when AOA dominate ammonia oxidation...
January 31, 2018: ISME Journal
https://www.readbyqxmd.com/read/29381740/variation-in-grain-zn-concentration-and-the-grain-ionome-in-field-grown-indian-wheat
#19
Jaswant Singh Khokhar, Sindhu Sareen, Bhudeva Singh Tyagi, Gyanendra Singh, Lolita Wilson, Ian P King, Scott D Young, Martin R Broadley
Wheat is an important dietary source of zinc (Zn) and other mineral elements in many countries. Dietary Zn deficiency is widespread, especially in developing countries, and breeding (genetic biofortification) through the HarvestPlus programme has recently started to deliver new wheat varieties to help alleviate this problem in South Asia. To better understand the potential of wheat to alleviate dietary Zn deficiency, this study aimed to characterise the baseline effects of genotype (G), site (E), and genotype by site interactions (GxE) on grain Zn concentration under a wide range of soil conditions in India...
2018: PloS One
https://www.readbyqxmd.com/read/29380279/whither-human-ivf-fertilisable-oocytes-selected-on-the-basis-of-follicular-temperature
#20
EDITORIAL
Ronald H F Hunter, Fernando López-Gatius
Bearing in mind specific parallels between cow and human ovarian physiology, as noted in the manuscript, we have measured whether the temperature in a pre-ovulatory follicle is cooler than that in adjacent tissues. Using a novel approach not requiring anaesthetics or surgical procedures, we found that follicular fluid bathing cow oocytes shortly before ovulation is cooler than the neighbouring uterine surface and cooler than deep rectal temperature (the reference body temperature in cattle). By contrast, Graafian follicles of comparable size and ultrasonic image that do not subsequently ovulate do not have a reduced antral temperature...
January 29, 2018: Journal of Assisted Reproduction and Genetics
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