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https://www.readbyqxmd.com/read/28421902/the-role-of-ovarian-reserve-markers-in-prediction-of-clinical-pregnancy
#1
Ali G Zebitay, Orkun Cetin, Fatma F Verit, Seda Keskin, M Nafi Sakar, Sercin Karahuseyinoglu, Gulsah Ilhan, Sezai Sahmay
To evaluate the role of ovarian reserve markers in the prediction of clinical pregnancy and embryo transfer accomplishment among poor responder IVF applicants. 304 female poor responder IVF applicants were included in this prospective cohort study conducted at the IVF-unit. Antral follicle count, FSH, LH, E2, AMH and IVF outcomes were compared in pregnant and non-pregnant groups as well as in ET vs. non-ET groups. The number of retrieved oocytes was significantly correlated positively with AMH and AFC, and negatively with FSH and age...
May 2017: Journal of Obstetrics and Gynaecology: the Journal of the Institute of Obstetrics and Gynaecology
https://www.readbyqxmd.com/read/28420479/andrographolide-disrupts-meiotic-maturation-by-blocking-cytoskeletal-reorganisation-and-decreases-the-fertilisation-potential-of-mouse-oocytes
#2
Hong-Xing Liang, Sheng-Sheng Lu, Zheng Yan, Yan-Ping Kuang, Xiang-Xing Zhu, Zhi-Guang Yan, Tong Du, Wei-Ran Chai, Hui Long, Qi-Feng Lyu
Andrographolide (AG) is a diterpenoid lactone isolated from the stem and leaves of Andrographis paniculata Nees that is used for the effective treatment of infectious diseases in Asian countries. Previous studies have reported adverse effects of AG on female fertility in rodents; however, the underlying mechanisms are unknown. The aim of the present study was to investigate the effects of AG on the IVM of mouse oocytes and their fertilisation potential. Immature oocytes incubated for 6, 14 or 24h in medium containing 5, 10 or 20μM AG showed time- and dose-dependent decreases in maturation rates compared with the control group...
April 19, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28419910/endometriosis-doubles-odds-for-miscarriage-in-patients-undergoing-ivf-or-icsi
#3
Corinna Pallacks, Jens Hirchenhain, Jan-Steffen Krüssel, Tanja N Fehm, Daniel Fehr
OBJECTIVE: To identify and estimate the importance of risk factors on pregnancy loss until the end of the second trimester after clinical pregnancy was achieved by either in vitro fertilisation (IVF) or intra-cytoplasmic sperm injection (ICSI). STUDY DESIGN: Retrospective cohort study including 588 cycles with fresh embryo transfers and 150 cycles with frozen-thawed embryo transfers using logistic regression. RESULTS: The rate of miscarriages subsequent to a fresh embryo transfer was significantly increased by a diagnosis of endometriosis (p=0...
April 5, 2017: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://www.readbyqxmd.com/read/28419104/further-characterisation-of-differences-between-tl-and-ab-zebrafish-danio-rerio-gene-expression-physiology-and-behaviour-at-day-5-of-the-larval-stage
#4
Ruud van den Bos, Wouter Mes, Pietro Galligani, Anthony Heil, Jan Zethof, Gert Flik, Marnix Gorissen
Zebrafish (Danio rerio) have become popular as model organism in research. Many strains are readily available, which not only differ morphologically, but also genetically, physiologically and behaviourally. Here, we focus on the AB and Tupfel long-fin (TL) strain for which we have previously shown that adults differ in baseline hypothalamus-pituitary-interrenal (HPI)-axis activity (AB higher than TL) affecting inhibitory avoidance behaviour (absent in AB). To assess whether strain differences are already present in early life stages, we compared baseline HPI-axis related gene expression as well as cortisol levels, (neuro)development related as well as (innate) immune system related gene expression, and light-dark as well as startle behaviour in larvae 5 days post fertilisation...
2017: PloS One
https://www.readbyqxmd.com/read/28410750/assessing-the-impact-of-diclofenac-ibuprofen-and-sildenafil-citrate-viagra-%C3%A2-on-the-fertilisation-biology-of-broadcast-spawning-marine-invertebrates
#5
Norlaila Binti Mohd Zanuri, Matthew G Bentley, Gary S Caldwell
Exposure to synthetic chemicals is a key environmental challenge faced by aquatic organisms. The time and dose effects of the pharmaceuticals diclofenac, ibuprofen, and sildenafil citrate on sperm motility and successful fertilisation are studied using the echinoderms, Asterias rubens and Psammechinus miliaris, and the polychaete worm Arenicola marina, all important components of the marine benthos. Motility was reduced for all species when exposed to diclofenac concentrations ≥0.1 μg/L. Exposure to ≥1...
April 8, 2017: Marine Environmental Research
https://www.readbyqxmd.com/read/28409413/impact-of-ammonium-nitrate-and-sodium-nitrate-on-tadpoles-of-alytes-obstetricans
#6
Núria Garriga, A Montori, G A Llorente
The presence of pesticides, herbicides and fertilisers negatively affect aquatic communities in general, and particularly amphibians in their larval phase, even though sensitivity to pollutants is highly variable among species. The Llobregat Delta (Barcelona, Spain) has experienced a decline of amphibian populations, possibly related to the reduction in water quality due to the high levels of farming activity, but also to habitat loss and alteration. We studied the effects of increasing ammonium nitrate and sodium nitrate levels on the survival and growth rate of Alytes obstetricans tadpoles under experimental conditions...
April 13, 2017: Ecotoxicology
https://www.readbyqxmd.com/read/28405052/estimating-the-enduring-effects-of-fertiliser-subsidies-on-commercial-fertiliser-demand-and-maize-production-panel-data-evidence-from-malawi
#7
Jacob Ricker-Gilbert, T S Jayne
Most studies of input subsidy programmes confine their analyses to measuring programme effects over a one-year period. This article estimates the potential longer-run or enduring effects of fertiliser subsidy programmes on smallholder farm households' demand for commercial fertiliser and maize production over time. We use four waves of panel data on 462 farm households in Malawi for whom fertiliser use can be tracked for eight consecutive seasons between 2003/2004 and 2010/2011. Panel estimation methods are used to control for potential endogeneity of subsidised fertiliser acquisition...
February 2017: J Agric Econ
https://www.readbyqxmd.com/read/28403915/the-egg-coat-zona-pellucida-3-glycoprotein-evolution-of-its-putative-sperm-binding-region-in-old-world-murine-rodents-rodentia-muridae
#8
Christine A Swann, Steven J B Cooper, William G Breed
In eutherian mammals, before fertilisation can occur the spermatozoon has to bind to, and penetrate, the egg coat, the zona pellucida (ZP). In the laboratory mouse there is good evidence that the primary sperm-binding site is a protein region encoded by Exon 7 of the ZP3 gene and it has been proposed that binding is species specific and evolves by sexual selection. In the present study we investigate these hypotheses by comparing Exon 6 and 7 sequences of ZP3 in 28 species of murine rodents of eight different divisions from Asia, Africa and Australasia, in which a diverse array of sperm morphologies occurs...
April 13, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28395188/ultrastructural-morphology-of-the-envelope-of-dover-sole-solea-solea-eggs-from-fertilization-until-hatching-with-emphasis-on-sample-preparation
#9
Evelien De Swaef, Myriam Claeys, Wim Bert, Ann Huysseune, Paul Eckhard Witten, Wim Van den Broeck, Annemie Decostere
This study is the first to describe the ultrastructural morphology of the envelope of Solea solea eggs from fertilisation until hatching. Defining the ultrastructural morphology of fish eggs is important for species identification and may assist in predicting the effect of external influences on these early life stages. In first instance, various fixation and embedding protocols were assessed to explore the morphology of the egg envelope, whereby the encountered difficulties were highlighted. The successful protocol for SEM proved to be combined fixation with 4% glutaraldehyde in 0...
March 27, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/28393578/kisspeptin-as-a-promising-oocyte-maturation-trigger-for-in-vitro-fertilisation-in-humans
#10
Miro Kasum, Daniela Franulić, Ermin Čehić, Slavko Orešković, Albert Lila, Emina Ejubović
The aim of this review is to analyse the effectiveness of exogenous kisspeptin administration as a novel alternative of triggering oocyte maturation, instead of currently used triggers such as human chorionic gonadotropin (hCG) or gonadotropin releasing hormone (GnRH) agonist, in women undergoing in vitro fertilisation (IVF) treatment. Kisspeptin has been considered a master regulator of two modes of GnRH and hence gonadotropin secretion, pulses and surges. Administration of kisspeptin-10 and kisspeptin-54 induces the luteinising hormone (LH) surge required for egg maturation and ovulation in animal investigations and LH release during the preovulatory phase of the menstrual cycle and hypothalamic amenorrhoea in humans...
April 10, 2017: Gynecological Endocrinology
https://www.readbyqxmd.com/read/28391738/acupuncture-in-reproductive-medicine-the-motivations-of-infertile-women-to-participate-in-a-randomised-controlled-trial
#11
Sheryl L de Lacey, Elizabeth Sanderman, Caroline A Smith
INTRODUCTION: Randomised controlled trials are the gold standard in medical research and are challenging to conduct successfully since high numbers of participants are needed to produce robust results. Therefore, it is important to understand what motivates patients to participate in one, particularly in Reproductive Medicine where the conduct of RCTs is rare. Just as it is important to evaluate medical interventions, it is equally important that adjuvant therapies are properly assessed...
April 10, 2017: Journal of Psychosomatic Obstetrics and Gynaecology
https://www.readbyqxmd.com/read/28390470/supplementation-of-culture-medium-with-l-carnitine-improves-the-development-and-cryotolerance-of-in-vitro-produced-porcine-embryos
#12
J L Lowe, L K Bartolac, R Bathgate, C G Grupen
Porcine oocytes and embryos contain substantial amounts of lipid, with little known regarding its metabolic role during development. This study investigated the role of lipid metabolism and the interaction between carbohydrate and lipid substrates in porcine embryos. Following in vitro fertilisation, presumptive zygotes were transferred to culture medium supplemented with L-carnitine, a co-factor required for the metabolism of fatty acids. In porcine zygote medium-3 (PZM-3), which contains pyruvate and lactate, 3mM L-carnitine was the only dose that improved cleavage rates compared with the control...
April 10, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28386985/endometriosis-an-everlasting-challenge
#13
Mette Haase Moen
Endometriosis is one of the most common benign gynecological diseases affecting 5-10% of women of fertile age, and the condition has received much attention. In spite of this, there are still challenges concerning diagnosing and treatment. The diagnostic delay can be more than ten years, and is especially long concerning teenagers. The symptoms can start already in adolescence, but this knowledge has not fully reached out to the medical profession, for example to general practitioners and pediatricians. As no hormonal treatment has proved to be superior, the least complicated drugs such as combined oral contraceptives or progestins should be the first choice for control of pain...
April 7, 2017: Acta Obstetricia et Gynecologica Scandinavica
https://www.readbyqxmd.com/read/28366969/impact-of-reduced-tillage-on-greenhouse-gas-emissions-and-soil-carbon-stocks-in-an-organic-grass-clover-ley-winter-wheat-cropping-sequence
#14
Maike Krauss, Reiner Ruser, Torsten Müller, Sissel Hansen, Paul Mäder, Andreas Gattinger
Organic reduced tillage aims to combine the environmental benefits of organic farming and conservation tillage to increase sustainability and soil quality. In temperate climates, there is currently no knowledge about its impact on greenhouse gas emissions and only little information about soil organic carbon (SOC) stocks in these management systems. We therefore monitored nitrous oxide (N2O) and methane (CH4) fluxes besides SOC stocks for two years in a grass-clover ley - winter wheat - cover crop sequence...
February 15, 2017: Agriculture, Ecosystems & Environment
https://www.readbyqxmd.com/read/28365678/the-effect-of-fertiliser-treatments-on-the-severity-of-fusarium-head-blight-and-mycotoxin-biosynthesis-in-winter-rye
#15
Bożena Cwalina-Ambroziak, Tomasz P Kurowski, Agnieszka Waśkiewicz, Piotr Goliński, Arkadiusz Stępień, Małgorzata Głosek-Sobieraj, Adam Perczak
The fungi of the genus Fusarium cause Fusarium head blight (FHB), a devastating disease that reduces grain yield and quality. They also produce mycotoxins which may pose a serious threat to human and animal health. This study investigated the effects of NPK fertilisation, foliar application of Cu, Zn, and Mn, applied separately and in combination, and of the Nano-Gro® organic growth stimulator on the occurrence of FHB in cultivar Dańkowskie Diament rye based on the mycological analysis of kernels and on the concentrations of Fusarium mycotoxins in grain...
March 1, 2017: Arhiv za Higijenu Rada i Toksikologiju
https://www.readbyqxmd.com/read/28365513/relationships-between-soil-parameters-and-physiological-status-of-miscanthus-x-giganteus-cultivated-on-soil-contaminated-with-trace-elements-under-npk-fertilisation-vs-microbial-inoculation
#16
Marta Pogrzeba, Szymon Rusinowski, Krzysztof Sitko, Jacek Krzyżak, Aleksandra Skalska, Eugeniusz Małkowski, Dorota Ciszek, Sebastian Werle, Jon Paul McCalmont, Michal Mos, Hazem M Kalaji
Crop growth and development can be influenced by a range of parameters, soil health, cultivation and nutrient status all play a major role. Nutrient status of plants can be enhanced both through chemical fertiliser additions (e.g. N, P, K supplementation) or microbial fixation and mobilisation of naturally occurring nutrients. With current EU priorities discouraging the production of biomass on high quality soils there is a need to investigate the potential of more marginal soils to produce these feedstocks and the impacts of soil amendments on crop yields within them...
March 30, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28364649/biochar-and-enhanced-phosphate-capture-mapping-mechanisms-to-functional-properties
#17
Jessica G Shepherd, Stephen Joseph, Saran P Sohi, Kate V Heal
A multi-technique analysis was performed on a range of biochar materials derived from secondary organic resources and aimed at sustainable recovery and re-use of wastewater phosphorus (P). Our purpose was to identify mechanisms of P capture in biochar and thereby inform its future optimisation as a sustainable P fertiliser. The biochar feedstock comprised pellets of anaerobically digested sewage sludge (PAD) or pellets of the same blended in the ratio 9:1 with ochre sourced from minewater treatment (POCAD), components which have limited alternative economic value...
July 2017: Chemosphere
https://www.readbyqxmd.com/read/28359014/sperm-motility-activation-in-the-critically-endangered-booroolong-frog-the-effect-of-medium-osmolality-and-phosphodiesterase-inhibitors
#18
Aimee J Silla, Leesa M Keogh, Phillip G Byrne
Effective activation of sperm motility is fundamental to successful artificial fertilisation; however, studies investigating optimal procedures in amphibians are lacking. This study found the optimal osmolality of activation media for sperm motility activation and evaluated the effect of phosphodiesterase (PDE) inhibitors on sperm activation and longevity in the critically endangered booroolong frog, Litoria booroolongensis. To assess the effect of medium osmolality (10, 25, 50, 75, 100 and 200mOsmolkg-1) and PDE inhibitors (control, 2...
March 31, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28355309/the-differential-expression-of-mc1r-regulators-in-dorsal-and-ventral-quail-plumages-during-embryogenesis-implications-for-plumage-pattern-formation
#19
Thanh-Lan Gluckman, Nicholas I Mundy
Melanin pigmentation patterns are ubiquitous in animals and function in crypsis, physical protection, thermoregulation and signalling. In vertebrates, pigmentation patterns formed over large body regions as well as within appendages (hair/feathers) may be due to the differential distribution of pigment producing cells (melanocytes) and/or regulation of the melanin synthesis pathway. We took advantage of the pigmentation patterns of Japanese quail embryos (pale ventrum and patterned feathers dorsally) to explore the role of genes and their transcripts in regulating the function of the melanocortin-1-receptor (MC1R) via 1...
2017: PloS One
https://www.readbyqxmd.com/read/28349525/glucocorticoid-supplementation-during-ovarian-stimulation-for-ivf-or-icsi
#20
REVIEW
Theodoros Kalampokas, Zabeena Pandian, Stephen D Keay, Siladitya Bhattacharya
BACKGROUND: Ovarian response to stimulation during in-vitro fertilisation (IVF) and intra-cytoplasmic sperm injection (ICSI) plays an important role in determining live birth rates. Adjuvant treatments during ovarian stimulation that have different modes of action have been used to improve ovarian response to stimulation and outcome of IVF. Glucocorticoids (GCs) are a class of steroid hormones that have been used either alone or in combination with other stimulatory regimens in order to improve folliculogenesis and pregnancy rates...
March 27, 2017: Cochrane Database of Systematic Reviews
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