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Ahmed Moustafa, Weizhong Li, Harinder Singh, Kelvin J Moncera, Manolito G Torralba, Yanbao Yu, Oriol Manuel, William Biggs, J Craig Venter, Karen E Nelson, Rembert Pieper, Amalio Telenti
Urine culture and microscopy techniques are used to profile the bacterial species present in urinary tract infections. To gain insight into the urinary flora, we analyzed clinical laboratory features and the microbial metagenome of 121 clean-catch urine samples. 16S rDNA gene signatures were successfully obtained for 116 participants, while metagenome sequencing data was successfully generated for samples from 49 participants. Although 16S rDNA sequencing was more sensitive, metagenome sequencing allowed for a more comprehensive and unbiased representation of the microbial flora, including eukarya and viral pathogens, and of bacterial virulence factors...
March 12, 2018: Scientific Reports
Laura Morris
A fussy 6-month-old infant is brought into the emergency department with a rectal temperature of 101.5 degrees F. She is consolable, breathing normally, and appears well hydrated. You find no clear etiology for her fever and suspect that a urinary tract infection may be the source of her illness. How do you proceed with obtaining a urine sample?
March 2018: Journal of Family Practice
Kiren Gill, Ryoon Kang, Sanchutha Sathiananthamoorthy, Rajvinder Khasriya, James Malone-Lee
INTRODUCTION AND HYPOTHESIS: This study sought to characterise the microbial ecology of the lower urinary tract in patients with symptoms of overactive bladder (OAB) using culture of the urinary urothelial cell sediment. The pathological significance of the microbiome was assessed through its relationship with known urothelial inflammatory markers and patient reported symptoms. METHODS: Adult female patients with OAB symptoms and asymptomatic controls were assessed at 12 study visits scheduled every 4 weeks...
February 17, 2018: International Urogynecology Journal
Wubalem Desta Seifu, Alemayehu Desalegn Gebissa
BACKGROUND: Urinary tract infection (UTI) remains to be one of the most common infectious diseases diagnosed in developing countries. And a widespread use of antibiotics against uropathogens has led to the emergence of antibiotic resistant species. A laboratory based cross-sectional survey was conducted in Shashemene referral hospital to determine the prevalence and antibiotic susceptibility of uropathogens. METHODS: We have collected 384 clean catch mid-stream urine samples from all suspected UTI outpatients using sterile screw capped container...
January 10, 2018: BMC Infectious Diseases
Astha Panghal, Aakash Pandita, Girish Gupta, Kirti Naranje, Anita Singh
A recent paper by Herreros et al in Acta Paediatrica reported, for the first time, that performing a dipstick test on a clean-catch urine sample was an accurate way of diagnosing a urinary tract infection (UTI) in a febrile infant under 90 days of age (1), without the need for invasive procedures. We have a number of comments on this study. It would have been useful to know how old the youngest infants were and the duration of unexplained fever. In addition, they used low-risk Rochester criteria to select the infants and one criterion is normal urinalysis, which may have affected the consistency of the study...
December 27, 2017: Acta Paediatrica
Angus N Oli, Vivian B Akabueze, Chijioke E Ezeudu, George U Eleje, Obiora S Ejiofor, Ifeanyichukwu U Ezebialu, Charlotte B Oguejiofor, Ifeoma M Ekejindu, George O Emechebe, Kenneth N Okeke
Background: Urinary Tract Infection (UTI) is a common contagion among men and women with the incidence relatively higher among women due to their differing anatomy. An understanding of the kind of pathogens implicated in urinary tract infections as well as antibiotic susceptibility profiling may help the clinician make rationally correct empirical choice in their treatment. Objective: This study is aimed at determining the type and antibiotic susceptibility pattern of bacterial uropathogens isolated from female patients attending Chukwuemeka Odumegwu Ojukwu University Teaching Hospital (COOUTH), Awka, Nigeria...
2017: Open Microbiology Journal
Shannon Hall-Million, Patricia Kunz Howard
Review of recent evidence with translation to practice for the advanced practice nurse (APN) role is presented using a case study module for "Faster Clean Catch Urine Collection (Quick Wee Method) From Infants: Randomised Controlled Trial." This prospective study enrolled 344 infants between 1 and 12 months of age seeking care in an emergency department with a complaint that led to the ordering of a clean catch urine specimen. Use of the Quick Wee procedure yielded clean catch urine specimens without the use of an invasive procedure...
October 2017: Advanced Emergency Nursing Journal
Alireza Esmailzadeh, Hossein Zarrinfar, AbdolMajid Fata, Tanuka Sen
BACKGROUND: Among the fungal pathogens, Candida species are the most common cause of urinary tract infection (UTI). Some predisposing factors such as diabetes mellitus, urinary retention, urinary stasis, renal transplantation, and hospitalization can increase the risk of candiduria. The aim of this cross-sectional study was to evaluate candiduria among type 2 diabetic patients and identification of the Candida isolates. METHOD: Four hundred clean-catch midstream urine specimens were obtained from patients with type 2 diabetes mellitus...
October 27, 2017: Journal of Clinical Laboratory Analysis
María Luisa Herreros, Alfredo Tagarro, Araceli García-Pose, Aida Sánchez, Alfonso Cañete, Pablo Gili
AIM: This study evaluated using urine dipstick tests with the clean-catch method to screen for urinary tract infection (UTI) in febrile infants under 90 days of age. METHODS: We carried out a comparative diagnostic accuracy study of infants under 90 days old, who were studied for unexplained fever without any source, in the emergency room of a hospital in Madrid from January 2011 to January 2013. We obtained matched samples of urine using two different methods: a clean-catch, standardised stimulation technique and catheterisation collection...
September 23, 2017: Acta Paediatrica
Aswathy Suresh, Anusha Gopinathan, Kavitha R Dinesh, Anil Kumar
INTRODUCTION: Urine antimicrobial activity is a seldom analysed laboratory test which greatly impacts the quantification of urine specimens. Presence of antimicrobial activity in the urine reduces the bacterial load in these specimens. Hence, the chances of erroneously reporting insignificant bacteriuria can be reduced on analysis of the antimicrobial activity in urine. AIM: The aim of the study was to measure the antimicrobial activity of urine samples obtained from patients in a tertiary care hospital...
July 2017: Journal of Clinical and Diagnostic Research: JCDR
Edmund Azah, Hwidong Kim, Timothy Townsend
Wastes generated from municipal cleaning activities such as street sweeping, ditch cleaning, stormwater pond maintenance, and catch basin sediment removal require appropriate management. Beneficial use of these types of waste is a good alternative to landfilling; however, there are genuine concerns about possible soil and groundwater contamination by pollutants such as polycyclic aromatic hydrocarbons (PAHs). This study assessed the potential risks associated with beneficial use of roadway and stormwater system residuals collected from 14 cities across the state of Florida, USA...
December 31, 2017: Science of the Total Environment
Mary S Jacob, Paige Kulie, Cameron Benedict, Alexander J Ordoobadi, Neal Sikka, Erika Steinmetz, Melissa L McCarthy
OBJECTIVES: Urine microscopy is a common test performed in emergency departments (EDs). Urine specimens can easily become contaminated by different factors, including the collection method. The midstream clean-catch (MSCC) collection technique is commonly used to reduce urine contamination. The urine culture contamination rate from specimens collected in our ED is 30%. We developed an instructional application (app) to show ED patients how to provide a MSCC urine sample. We hypothesized that ED patients who viewed our instructional app would have significantly lower urine contamination rates compared to patients who did not...
January 2018: American Journal of Emergency Medicine
Juliana Schroeder, Ayelet Fishbach, Chelsea Schein, Kurt Gray
Intimacy is often motivated by love, but sometimes it is merely functional. For example, disrobing and being touched at an airport security check serves the goal of catching a flight, not building a relationship. We propose that this functional intimacy induces discomfort, making people prefer greater social distance from their interaction partner. Supporting this prediction, participants who considered (Experiments 1 and 2) or experienced (Experiment 3) more physically intimate medical procedures preferred a health provider who is less social...
December 2017: Journal of Personality and Social Psychology
Shidan Tosif, Jonathan Kaufman, Patrick Fitzpatrick, Sandy M Hopper, Monsurul Hoq, Susan Donath, Franz E Babl
AIM: Clean catch urine (CCU) collection is commonly used in pre-continent children. CCU can be time-consuming and specimens may be contaminated. Our aim was to determine the time taken for CCU attempts and to describe the success of this method in diagnosing or excluding urinary tract infection. METHODS: Prospective observational study of CCU in pre-continent children aged 2-48 months in the emergency department. Time taken until urine collection, 'successful' (voided and caught), 'missed' (voided not caught) or the procedure 'stopped', were recorded and urine culture results analysed...
June 15, 2017: Journal of Paediatrics and Child Health
Antonioni A C Moliterno, Camila B C Martins, Daiane Szczerbowski, Maria Aparecida C Zawadneak, Paulo H G Zarbin
The nitidulid beetle Lobiopa insularis is an important pest of strawberry crops in the United States and Brazil. Both larvae and adults feed on ripe strawberries, causing 20-70% loss in production during serious infestations. Aiming at the development of efficient, clean, and highly specific pest management systems, semiochemicals, especially pheromones, are particularly useful. Analyses of the extracts of both males and females obtained from aeration of live beetles showed the presence of three male specific compounds, 2-nonanone, 2-undecanone, and 2-undecanol (in an enantiomeric ratio of S:R = 3...
June 2017: Journal of Chemical Ecology
Michael R Polin, Amie Kawasaki, Cindy L Amundsen, Alison C Weidner, Nazema Y Siddiqui
OBJECTIVES: To determine whether mixed-flora preoperative urine cultures, as compared with no-growth preoperative urine cultures, are associated with a higher prevalence of postoperative urinary tract infections (UTIs). METHODS: This was a retrospective cohort study. Women who underwent urogynecologic surgery were included if their preoperative clean-catch urine culture result was mixed flora or no growth. Women were excluded if they received postoperative antibiotics for reasons other than treatment of a UTI...
June 2017: Southern Medical Journal
Timothy J Suchomel, Jason P Lake, Paul Comfort
The purpose of this study was to compare the load absorption force-time characteristics of weightlifting catching and pulling derivatives. Twelve resistance-trained men performed repetitions of the hang power clean (HPC), jump shrug (JS), and hang high pull (HHP) on a force platform with 30, 45, 65, and 80% of their 1-repetition maximum HPC. Load absorption phase duration, mean force, and work were calculated from the force-time data. The HHP produced a significantly longer load absorption phase duration compared with the HPC (p < 0...
June 2017: Journal of Strength and Conditioning Research
Saera Suhail Kidwai, Ayesha Nageen, Samina Ghaznavi, Farhat Bashir, Jamal Ara
BACKGROUND AND OBJECTIVE: Urinary tract infection is one of the commonest infections seen in clinical practice. Lack of compliance and unjustified antibiotic prescriptions has resulted in bacterial resistance and is proving as a major challenge in the management of these infections. Our aim was to identify the sensitivity pattern of commonly used antibiotics against urinary tract infections so as to suggest an improvised line of action against bacteria causing urinary tract infections'...
March 2017: Pakistan Journal of Medical Sciences Quarterly
A Kilonback
No abstract text is available yet for this article.
April 7, 2017: BMJ: British Medical Journal
Jonathan Kaufman, Patrick Fitzpatrick, Shidan Tosif, Sandy M Hopper, Susan M Donath, Penelope A Bryant, Franz E Babl
Objective  To determine if a simple stimulation method increases the rate of infant voiding for clean catch urine within five minutes. Design  Randomised controlled trial. Setting  Emergency department of a tertiary paediatric hospital, Australia. Participants  354 infants (aged 1-12 months) requiring urine sample collection as determined by the treating clinician. 10 infants were subsequently excluded. Interventions  Infants were randomised to either gentle suprapubic cutaneous stimulation (n=174) using gauze soaked in cold fluid (the Quick-Wee method) or standard clean catch urine with no additional stimulation (n=170), for five minutes...
April 7, 2017: BMJ: British Medical Journal
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