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https://www.readbyqxmd.com/read/28635397/induction-of-apoptosis-and-autophagy-by-calcifying-nanoparticles-in-human-bladder-cancer-cells
#1
Ji-Hua Wu, Yao-Liang Deng, Quan Liu, Jun-Chuan Yu, Yun-Long Liu, Zi-Qi He, Xiao-Feng Guan
Calcifying nanoparticles have been linked to various types of human disease, but how they contribute to disease processes is unclear. Here, we examined whether and how calcifying nanoparticles isolated from patients with kidney stones are cytotoxic to human bladder cancer cells. Calcifying nanoparticles were isolated from midstream urine of patients with renal calcium oxalate stones and examined by electron microscopy. Human bladder cancer cells (EJ cells) were cultured in the presence of calcifying nanoparticles or nanohydroxyapatites for 12 and 72 h and examined for toxicity using the Cell Counting Kit-8, for autophagy using transmission electron microscopy and confocal microscopy, and for apoptosis using fluorescence microscopy, transmission electron microscopy, and flow cytometry...
June 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/28601118/determination-of-red-blood-cells-in-urinary-sediment-do-ph-and-specific-gravity-of-urine-matter
#2
Nuša Avguštin, Alexander Jerman, Žiga Rotar, Špela Borštnar, Andrej Škoberne, Jelka Lindič
INTRODUCTION: It has been postulated that erythrocyte cell lysis in urine is common in the case of low urine specific gravity and high urine pH. We aimed to verify the influence of urine dipstick pH and specific gravity on erythrocyturia in the urine sediment in the case of positive dipstick hemoglobinuria. METHODS: We retrospectively collected data on dipstick specific gravity, pH, and urine sediment analysis done by nephrologists in the clinical and research urine laboratory at the Department of Nephrology, University Medical Center Ljubljana...
June 9, 2017: Clinical Nephrology
https://www.readbyqxmd.com/read/28587474/pollution-by-nonylphenol-in-river-tap-water-and-aquatic-in-an-acid-rain-plagued-city-in-southwest-china
#3
Yu Jie, Zhou Jie, Luo Ya, Yang Xuesong, Yang Jing, Yang Yu, Yang Jiaqi, Xu Jie
OBJECTIVE: Nonylphenol (NP) has provoked much environmental concern because of their weak estrogenic activities; however, few data are available on the environmental levels of the chemical in China. METHOD: Environmental or river samples were assayed for NP by high-performance liquid chromatography (HPLC) technique. RESULTS: The concentration for NP measured in Xiangjiang River, ranging from 0.174 to 3.411 μg/L with a mean value of 1.73 μg/L, was lower than the Water Quality Criteria for NP of the US (6...
June 6, 2017: International Journal of Environmental Health Research
https://www.readbyqxmd.com/read/28569232/relationship-among-phosphorus-content-in-water-sediment-interface-in-middle-temperate-grassland
#4
E Jing Su Derong Lv Shihai Diao Zhaoyan Bu He Zheng Zhirong Ye Shengxing
In order to reveal the dynamic changes of phosphorus release from the sediments of the wetlands in mid-temperature steppe without external phosphorus input, the relationship among the phosphorus content in the overlying water, sediment and interstitial water was studied by using variables control method. In July of 2015, the undisturbed sediments and corresponding overlying water from the sediment-water interface of Hulunbeier steppe, China were selected (included Yimin River midstream (YM) wetland, Muqiao section (HM) wetland, Swan Lake (TE) wetland, Xiboqiao section (HX) wetland, Huihe and Yi Min River Interchange (HY) wetland and Hulun lake (HH) wetland) and cultured in wetland habitat simulator...
May 18, 2017: Water Environment Research: a Research Publication of the Water Environment Federation
https://www.readbyqxmd.com/read/28533857/antibiogram-profile-of-uropathogens-isolated-at-bahir-dar-regional-health-research-laboratory-centre-northwest-ethiopia
#5
Awoke Derbie, Derese Hailu, Daniel Mekonnen, Bayeh Abera, Gashaw Yitayew
INTRODUCTION: Antimicrobial resistance among bacteria that cause urinary tract infection (UTI) has been increasing since the introduction of chemotherapy. This study was aimed to assess the types of isolates from patients with UTI and to determine their current antimicrobial susceptibility profile. METHODS: A record based retrospective analysis of bacterial uropathogens processed in the period of January 2012 to December 2014 at Bahir Dar Health Research Laboratory Center (BRHRLC) was determined...
2017: Pan African Medical Journal
https://www.readbyqxmd.com/read/28525997/increased-resistance-of-gram-negative-urinary-pathogens-after-kidney-transplantation
#6
Johannes Korth, Julia Kukalla, Peter-Michael Rath, Sebastian Dolff, Marco Krull, Hana Guberina, Anja Bienholz, Benjamin Wilde, Stefan Becker, Birgit Ross, Olympia Evdoxia Anastasiou, Andreas Kribben, Oliver Witzke
BACKGROUND: Urinary tract infection is the most common complication after kidney transplantation. It can cause severe sepsis and transplant loss. Emergence of drug resistance among gram-negative urinary pathogens is the current challenge for urinary tract infection treatment after kidney transplantation. METHODS: This study analyzes the antimicrobial susceptibility of gram-negative urinary pathogens after kidney transplantation from 2009 to 2012 at the Transplant Outpatient Clinic of the University Hospital Essen, Germany...
May 19, 2017: BMC Nephrology
https://www.readbyqxmd.com/read/28523017/antibiotic-susceptibility-in-commonly-isolated-pathogens-from-urinary-tract-infection-in-a-cohort-of-subjects-from-low-socioeconomic-strata
#7
Saera Suhail Kidwai, Ayesha Nageen, Samina Ghaznavi, Farhat Bashir, Jamal Ara
BACKGROUND AND OBJECTIVE: Urinary tract infection is one of the commonest infections seen in clinical practice. Lack of compliance and unjustified antibiotic prescriptions has resulted in bacterial resistance and is proving as a major challenge in the management of these infections. Our aim was to identify the sensitivity pattern of commonly used antibiotics against urinary tract infections so as to suggest an improvised line of action against bacteria causing urinary tract infections'...
March 2017: Pakistan Journal of Medical Sciences Quarterly
https://www.readbyqxmd.com/read/28513840/histomorphology-and-innate-immunity-during-the-progression-of-osteoarthritis-does-synovitis-affect-cartilage-degradation
#8
Huan Wang, Qingguo Wang, Meijuan Yang, Lili Yang, Weili Wang, Haobin Ding, Dong Zhang, Jing Xu, Xuezhang Tang, Haitao Ding, Qingfu Wang
Osteoarthritis (OA) is a common chronic degenerative disease that affects all joints. At present, the pathological processes and mechanisms of OA are still unclear. Innate immunity, a key player in damage to the structure of the joint and the mechanism by which the host attempts to repair OA, affects all pathological stages of the disease. In the present study, our aim was to assess changes in innate immunity during the pathological processes of OA in articular cartilage (AC) and the synovial membrane (SM), which are the major structures in joints, and to systematically examine the histological changes in AC and SM in mild, moderate and severe cases of OA, in order to further speculate about the manner in which the interactions of AC and SM are facilitated by innate immunity...
May 17, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28511489/micronucleus-assay-in-urothelial-cells-in-cancer-cervix
#9
Suresh Kumar Sundararajan, Pratheepa Sivasankari Natarajan, Kanchana
INTRODUCTION: Cancer ranks third among the ten leading global causes of death. To evaluate the genotoxic risks, observed as DNA damages, can be assessed by Micronucleus (MN) test. AIM: To identify the occurrence of MN in normal and cancer cervix and find the correlation between MN and stage of cancer. MATERIALS AND METHODS: A total of 60 females were included in the study and visual examination of the cervix was done. Based on the examination two groups were formed: A- Normal cervix (n-23) and B- Presence of erosion or growth or ulcer etc...
March 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28475170/urinary-tract-infections-in-postmenopausal-women-with-type-2-diabetes-clinical-correlates-and-quinolone-susceptibility
#10
Martyna Borowczyk, Anna Chmielarz-Czarnocińska, Paula Faner, Andrzej Paciorkowski, Jan K Nowak, Ewelina Szczepanek-Parulska, Marek Ruchała, Maciej Cymerys
INTRODUCTION    Both diabetes mellitus and the postmenopausal period predispose to urinary tract infections (UTIs). However, the data on UTIs in postmenopausal diabetics are scarce.  OBJECTIVES    The aim was to determine the prevalence of UTIs in postmenopausal women with type 2 DM (T2DM), identify the potential risk factors, describe the causative pathogens, and assess their susceptibility to quinolones. PATIENTS AND METHODS    The patients were interviewed, examined, and had their hospital records analyzed...
May 5, 2017: Polish Archives of Internal Medicine
https://www.readbyqxmd.com/read/28451019/prevalence-aetiology-and-antibiotic-sensitivity-profile-of-asymptomatic-bacteriuria-isolates-from-pregnant-women-in-selected-antenatal-clinic-from-nairobi-kenya
#11
Adelaide Ogutu Ayoyi, Gideon Kikuvi, Christine Bii, Samuel Kariuki
INTRODUCTION: Asymptomatic bacteriuria (ASB) is the presence of bacteria in urine without apparent symptoms of urinary tract infections. The importance of asymptomatic bacteriuria lies in the insight it provides into symptomatic infections. To determine prevalence, bacterial isolates and Antibiotic Sensitivity Profile of asymptomatic bacterial urinary tract infection in pregnant women in selected clinics in Nairobi. METHODS: This was a cross-sectional study involving women attending antenatal clinic at selected clinics of Nairobi County...
2017: Pan African Medical Journal
https://www.readbyqxmd.com/read/28434051/evidence-of-a-link-between-fever-and-microscopic-hematuria-in-children
#12
Rama Schwartz, Rotem Distal, Arthur Shapiro, Yehezkel Waisman
Despite the common conception that febrile illnesses are associated with microscopic hematuria in children, a review of the literature failed to disclose supportive evidence. The aim of this study was to evaluate this relationship. Background data were collected for a convenience sample of children aged 4-18 years who presented to the emergency department (ED) of a tertiary pediatric medical center. Vital signs were measured and a midstream urine specimen was collected. Specimens positive for the presence of blood (>5 erythrocytes/μl; Combur 10 Test Strips) were examined microscopically...
April 22, 2017: European Journal of Pediatrics
https://www.readbyqxmd.com/read/28421253/levels-and-sources-of-organochlorine-pesticides-in-surface-sediment-from-anhui-reach-of-huaihe-river-china
#13
Chunnian Da, Ke Wu, Jie Jin, Minjun Yu
Twenty-two organochlorine pesticides (OCPs) were investigated in Anhui reach of Huaihe river, China. Seventeen out of 22 OCPs were detected by GC-MS. The mean concentrations of OCPs followed the order: HCHs > DDTs > HCB > chlordanes > endosulfans. Levels of total HCHs and total DDTs ranged from 2.54 to 13.91 ng g(-1) (mean = 7.52 ng g(-1)) and 0.016 to 2.54 ng g(-1) (mean = 0.45 ng g(-1)), respectively. The concentrations of DDTs were lower than those of HCHs. Compared with the other rivers in China, DDTs and HCHs were relatively lower or similar...
June 2017: Bulletin of Environmental Contamination and Toxicology
https://www.readbyqxmd.com/read/28420507/asymptomatic-pyuria-in-pregnant-women-during-the-first-trimester-is-associated-with-an-increased-risk-of-adverse-obstetrical-outcomes
#14
Yun-Ju Lai, Te-Yao Hsu, Kuo-Chung Lan, Hao Lin, Chia-Yu Ou, Hung-Chun Fu, Ching-Chang Tsai
OBJECTIVE: Urinalysis is included in the prenatal examination in the first trimester in Taiwan, in contrast to Western countries. We aimed to investigate whether asymptomatic pyuria as detected by urinalysis was associated with adverse perinatal outcomes. MATERIALS AND METHODS: A total of 1187 singleton pregnant women who received prenatal care at Kaohsiung Chang Gung Memorial Hospital between January 2012 and December 2013 were included for retrospective analysis...
April 2017: Taiwanese Journal of Obstetrics & Gynecology
https://www.readbyqxmd.com/read/28390962/in-vitro-activity-of-fosfomycin-trometamol-and-other-oral-antibiotics-against-multidrug-resistant-uropathogens
#15
Maria Lina Mezzatesta, Giulia La Rosa, Gaetano Maugeri, Tiziana Zingali, Carla Caio, Andrea Novelli, Stefania Stefani
Clinical midstream and urinary catheter isolates (n = 106) of extended-spectrum β-lactamase (ESBL)-positive Escherichia coli, Klebsiella pneumoniae carbapenemase (KPC)-producing Klebsiella pneumoniae, Proteus mirabilis and meticillin-resistant Staphylococcus saprophyticus were tested against fosfomycin using the agar dilution method, the broth microdilution method and the gradient test described by the Clinical and Laboratory Standards Institute. Nitrofurantoin, co-trimoxazole, amoxicillin/clavulanic acid, cefuroxime, levofloxacin and ciprofloxacin were tested using the gradient test alone...
June 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28389618/sleep-and-ckd-in-chinese-adults-a-cross-sectional-study
#16
Junjuan Li, Zhe Huang, Jinhong Hou, Amy M Sawyer, Zhijun Wu, Jianfang Cai, Gary Curhan, Shouling Wu, Xiang Gao
BACKGROUND AND OBJECTIVES: To assess the association between self-reported sleep duration and quality and odds of having CKD in Chinese adults on the basis of a community study. DESIGN, SETTING, PARTICIPANTS, & MEASUREMENTS: In this cross-sectional study, we included 11,040 Chinese adults who participated in an ongoing prospective study, the Kailuan cohort. Survey questionnaire items addressed insomnia, daytime sleepiness, snoring, and sleep duration during their 2012 interview...
June 7, 2017: Clinical Journal of the American Society of Nephrology: CJASN
https://www.readbyqxmd.com/read/28365845/assessing-the-effects-of-rural-livelihood-transition-on-non-point-source-pollution-a-coupled-abm-iecm-model
#17
Chengcheng Yuan, Liming Liu, Jinwei Ye, Guoping Ren, Dong Zhuo, Xiaoxing Qi
Water pollution caused by anthropogenic activities and driven by changes in rural livelihood strategies in an agricultural system has received increasing attention in recent decades. To simulate the effects of rural household livelihood transition on non-point source (NPS) pollution, a model combining an agent-based model (ABM) and an improved export coefficient model (IECM) was developed. The ABM was adopted to simulate the dynamic process of household livelihood transition, and the IECM was employed to estimate the effects of household livelihood transition on NPS pollution...
May 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28340803/evaluation-of-ureteral-stent-colonization-in-live-donor-renal-transplant-recipients
#18
M Sarier, M Demir, I Duman, Y Yuksel, A Demirbas
BACKGROUND: Ureteral stent insertion during kidney transplantation is a matter of debate. Stenting has been proven to reduce the risk of surgical complications. In addition, it has been reported to increase risks such as urinary tract infections especially after operation. Ureteral stent colonization (USC) is known to play a role in the pathogenesis of stent related-infections. The aim of this study was (1) to assess the frequency of USC and values of urine cultures in identifying colonizing bacteria; (2) to assess the importance of indwelling time for USC in live-donor renal transplant recipients; and (3) to evaluate the biomarker role of neutrophil-to-lymphocyte ratio (NLR) on USC...
April 2017: Transplantation Proceedings
https://www.readbyqxmd.com/read/28325856/prevalence-of-undiagnosed-asymptomatic-bacteriuria-and-associated-risk-factors-during-pregnancy-a-cross-sectional-study-at-two-tertiary-centres-in-cairo-egypt
#19
Mohamed Abdel-Aziz Elzayat, Ashton Barnett-Vanes, Mohamed Farag Elmorsy Dabour, Feng Cheng
BACKGROUND: The prevalence of asymptomatic bacteriuria (ASB) during pregnancy is poorly understood in Egypt-a country with a high birth rate. OBJECTIVES: To determine the prevalence of ASB among pregnant women booking at El Hussein and Sayed Galal Hospitals in Al-Azhar University in Egypt; and to observe the relationship between ASB prevalence and risk factors such as socioeconomic level and personal hygiene. SETTING: Obstetrics and gynaecology clinics of 2 university hospitals in the capital of Egypt...
March 21, 2017: BMJ Open
https://www.readbyqxmd.com/read/28316574/alterations-of-urinary-microbiota-in-type-2-diabetes-mellitus-with-hypertension-and-or-hyperlipidemia
#20
Fengping Liu, Zongxin Ling, Yonghong Xiao, Qing Yang, Baohong Wang, Li Zheng, Ping Jiang, Lanjuan Li, Wei Wang
Evidence shows urine specimens from different women have different populations of bacteria. The co-occurrence of hypertension and hyperlipidemia in those with diabetes may alter the composition of urine and the microenviroment of the bladder in which bacteria live. The aim of this study was to characterize the urinary microbiota in women with type 2 diabetes mellitus only and those with diabetes plus hypertension and/or hyperlipidemia, and to explore whether the composition of the urinary microbiota is affected by fasting blood glucose, blood pressure, and blood lipids...
2017: Frontiers in Physiology
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