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https://www.readbyqxmd.com/read/29122686/an-artificially-constructed-dimer-through-deformation-of-a-short-zinc-binding-loop
#1
Jimin Park, Mi-Sun Kim, Keehyung Joo, Jooyoung Lee, Dong Hae Shin
We have analyzed the crystal structure of the dimeric form of d-glycero-d-manno-heptose-1,7-bisphosphate phosphatase from Burkholderia thailandensis (BtGmhB), catalyzing the removal of the phosphate at the 7 position of d-glycero-d-manno-heptose-1,7-bisphosphate. The crystal structure of BtGmhB revealed a dimeric form caused by a disruption of a short zinc-binding loop. The dimeric BtGmhB structure was induced by triggering the loss of Zn(2+)via the protonation of cysteine residues at pH 4.8 of the crystallization condition...
November 6, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29099761/the-role-of-carbohydrates-in-the-lipopolysaccharide-lps-toll-like-receptor-4-tlr4-signalling
#2
REVIEW
Florent Cochet, Francesco Peri
The interactions between sugar-containing molecules from the bacteria cell wall and pattern recognition receptors (PRR) on the plasma membrane or cytosol of specialized host cells are the first molecular events required for the activation of higher animal's immune response and inflammation. This review focuses on the role of carbohydrates of bacterial endotoxin (lipopolysaccharide, LPS, lipooligosaccharide, LOS, and lipid A), in the interaction with the host Toll-like receptor 4/myeloid differentiation factor 2 (TLR4/MD-2) complex...
November 3, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29093680/mechanistic-study-of-bakuchiol-induced-anti-breast-cancer-stem-cell-and-in-vivo-anti-metastasis-effects
#3
Li Li, Chi C Liu, Xueping Chen, Shisan Xu, Sinai Hernandez Cortes-Manno, Shuk H Cheng
Cancer stem cells are involved in cancer establishment, progression, and resistance to current treatments. We demonstrated the in vitro and in vivo anti-breast cancer effect of bakuchiol in a previous study. However, the ability of bakuchiol to target breast cancer stem cells (BCSCs) and inhibit breast cancer metastasis remains unknown. In the current study, we used the cell surface markers CD44 and CD24 to distinguish BCSCs from MCF-7 cells. Bakuchiol inhibited mammosphere formation and aldehyde dehydrogenase activity in BCSCs...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29062238/structural-and-functional-characterization-of-the-helicobacter-pylori-cytidine-5-monophosphate-pseudaminic-acid-synthase-psef-molecular-insight-into-substrate-recognition-and-catalysis-mechanism
#4
Syeda Umme Habiba Wahid
The bacterium Helicobacter pylori is a human gastric pathogen that can cause a wide range of diseases, including chronic gastritis, peptic ulcer and gastric carcinoma. It is classified as a definitive (class I) human carcinogen by the International Agency for Research on Cancer. Flagella-mediated motility is essential for H. pylori to initiate colonization and for the development of infection in human beings. Glycosylation of the H. pylori flagellum with pseudaminic acid (Pse; 5,7-diacetamido-3,5,7,9-tetradeoxy-l-glycero-l-manno-nonulosonic acid) is essential for flagella assembly and function...
2017: Advances and Applications in Bioinformatics and Chemistry: AABC
https://www.readbyqxmd.com/read/29050611/structure-and-in-vitro-anticancer-activity-of-sulfated-o-polysaccharide-from-marine-bacterium-poseidonocella-pacifica-kmm-9010-t
#5
Maxim S Kokoulin, Alexandra S Kuzmich, Anatoly I Kalinovsky, Eugene S Rubtsov, Lyudmila A Romanenko, Valery V Mikhailov, Nadezhda A Komandrova
We presented the structure of the sulfated polysaccharide moiety and anticancer activity in vitro of the О-deacylated lipopolysaccharide (DPS) isolated from the marine bacterium Poseidonocella pacifica KMM 9010(T). The structure of O-polysaccharide was investigated by chemical methods along with (1)H and (13)C NMR spectroscopy. The O-polysaccharide was built up of sulfated disaccharide repeating units consisted of d-rhamnose (d-Rhaр) and 3-deoxy-d-manno-oct-2-ulosonic acid (Kdop): →7)-β-Kdoр4Ac5S-(2→3)-β-d-Rhaр2S-(1→...
December 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/29039672/stereoselective-epimerizations-of-glycosyl-thiols
#6
Lisa M Doyle, Shane O'Sullivan, Claudia Di Salvo, Michelle McKinney, Patrick McArdle, Paul V Murphy
Glycosyl thiols are widely used in stereoselective S-glycoside synthesis. Their epimerization from 1,2-trans to 1,2-cis thiols (e.g., equatorial to axial epimerization in thioglucopyranose) was attained using TiCl4, while SnCl4 promoted their axial-to-equatorial epimerization. The method included application for stereoselective β-d-manno- and β-l-rhamnopyranosyl thiol formation. Complex formation explains the equatorial preference when using SnCl4, whereas TiCl4 can shift the equilibrium toward the 1,2-cis thiol via 1,3-oxathiolane formation...
October 17, 2017: Organic Letters
https://www.readbyqxmd.com/read/29032659/in-silico-analysis-of-putative-drug-and-vaccine-target-of-the-metabolic-pathways-of-actinobacillus-pleuropneumoniae-using-subtractive-comparative-genomics-approach
#7
Biruk Tesfaye Birhanu, Seung-Jin Lee, Na-Hye Park, Ju-Beom Song, Seung-Chun Park
Actinobacillus pleuropneumoniae is a gram-negative bacterium residing in the respiratory tract of pigs that causes porcine respiratory disease complex, which leads to significant losses in the pig industry worldwide. The incidence of drug resistance in this bacterium is increasing; thus, identifying new protein/gene targets for drug and vaccine development is critical. In this study, we used an in silico approach, utilizing several databases, including Kyoto Encyclopedia of Genes and Genomes (KEGG), the database of essential genes (DEG), DrugBank, and Swiss-Prot, to identify non-homologous essential genes and prioritize these proteins for their druggability...
October 13, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/29031030/ectopic-bone-as-a-nidus-for-calcium-oxalate-urocystolithiasis-in-a-cat
#8
J P Lulich, H S Prasad, M Manno, B Bagley
A 7-year-old female spayed domestic shorthair cat was referred to the urology service for nonsurgical urocystolith removal. A urolith was attached to the urothelium by ectopic bone. Interventional removal without surgery was successful. Follow-up evaluation 3 years after urolith removal revealed recurrent uroliths, bladder wall mineralization, and marked renal mineralization. This case illustrates the metaplastic potential of the urothelium and that ectopic bone should be included among the pathological factors that promote lithogenesis...
October 14, 2017: Journal of Veterinary Internal Medicine
https://www.readbyqxmd.com/read/29028168/synthesis-of-a-pentasaccharide-fragment-related-to-the-inner-core-region-of-rhizobial-and-agrobacterial-lipopolysaccharides
#9
Nino Trattnig, Jean-Baptiste Farcet, Philipp Gritsch, Anna Christler, Ralph Pantophlet, Paul Kosma
The pentasaccharide fragment α-d-Man-(1 → 5)-[α-d-Kdo-(2 → 4)-]α-d-Kdo-(2 → 6)-β-d-GlcNAc-(1 → 6)-α-d-GlcNAc equipped with a 3-aminopropyl spacer moiety was prepared by a sequential assembly of monosaccharide building blocks. The glucosamine disaccharide-as a backbone surrogate of the bacterial lipid A region-was synthesized using an 1,3-oxazoline donor, which was followed by coupling with an isopropylidene-protected Kdo-fluoride donor to afford a protected tetrasaccharide intermediate. Eventually, an orthogonally protected manno-configured trichloroacetimidate donor was used to achieve the sterically demanding glycosylation of the 5-OH group of Kdo in good yield...
October 26, 2017: Journal of Organic Chemistry
https://www.readbyqxmd.com/read/29027241/spatial-distribution-of-kidney-disease-in-the-contaminated-site-of-taranto-italy
#10
Marta Benedetti, Marco De Santis, Valerio Manno, Sante Minerba, Antonella Mincuzzi, Angela Morabito, Nicola Panocchia, Maria Eleonora Soggiu, Annalisa Tanzarella, Tiziano Pastore, Maurizio Bossola, Roberto Giua, Simona Leogrande, Alessandra Nocioni, Susanna Conti, Pietro Comba
BACKGROUND: Exposure to heavy metals has been associated with kidney disease. We investigated the spatial distribution of kidney disease in the industrially contaminated site of Taranto. METHODS: Cases were subjects with a first hospital discharge diagnosis of kidney disease. Cases affected by specific comorbidities were excluded. Standardized Hospitalization Ratios (SHRs) were computed for low/high exposure area and for modeled spatial distribution of cadmium and fine particulate matter...
October 13, 2017: American Journal of Industrial Medicine
https://www.readbyqxmd.com/read/29026979/hpaec-pad-for-oligosaccharide-analysis-novel-insights-into-analyte-sensitivity-and-response-stability
#11
Matthias Mechelke, Jonathan Herlet, J Philipp Benz, Wolfgang H Schwarz, Vladimir V Zverlov, Wolfgang Liebl, Petra Kornberger
The rising importance of accurately detecting oligosaccharides in biomass hydrolyzates or as ingredients in food, such as in beverages and infant milk products, demands for the availability of tools to sensitively analyze the broad range of available oligosaccharides. Over the last decades, HPAEC-PAD has been developed into one of the major technologies for this task and represents a popular alternative to state-of-the-art LC-MS oligosaccharide analysis. This work presents the first comprehensive study which gives an overview of the separation of 38 analytes as well as enzymatic hydrolyzates of six different polysaccharides focusing on oligosaccharides...
October 12, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29024844/structural-characterisation-of-the-capsular-polysaccharide-expressed-by-burkholderia-thailandensis-strain-e555-wbii-pknock-kmr-and-assessment-of-the-significance-of-the-2-o-acetyl-group-in-immune-protection
#12
Marc Bayliss, Matthew I Donaldson, Sergey A Nepogodiev, Giulia Pergolizzi, Andrew E Scott, Nicholas J Harmer, Robert A Field, Joann L Prior
Burkholderia pseudomallei and its close relative B. mallei are human pathogens that are classified as Tier 1 bio-threat agents. Both organisms have previously been shown to constitutively produce a capsular polysaccharide (CPS) that is both a virulence determinant and protective antigen. Extraction and purification of CPS for use as a potential vaccine candidate requires containment level 3 laboratories which is expensive and time-consuming. B. thailandensis strain E555 is closely related to B. pseudomallei and B...
November 27, 2017: Carbohydrate Research
https://www.readbyqxmd.com/read/29022330/bladder-stone-management-an-update
#13
Antonio Cicione, Cosimo DE Nunzio, Stefano Manno, Rocco Damiano, Alessandro Posti, Estevao Lima, Andrea Tubaro, Filippo Balloni
INTRODUCTION: Bladder stone (BS) is a rare disease curable with several options. Herein, we reviewed the specific literature in order to update the current BS management. EVIDENCE ACQUISITION: A comprehensive systematic MEDLINE search was performed for English language reports published before April 2017 using the BS related terms, i.e. bladder-vesical calculi, lithotripsy. Then manuscripts references were screened to identify unfounded studies. Studies regarding BS in children were excluded...
October 11, 2017: Minerva Urologica e Nefrologica, the Italian Journal of Urology and Nephrology
https://www.readbyqxmd.com/read/29021148/the-voltage-sensor-of-excitation-contraction-coupling-in-mammals-inactivation-and-interaction-with-ca-2
#14
Juan Ferreira Gregorio, Germán Pequera, Carlo Manno, Eduardo Ríos, Gustavo Brum
In skeletal muscle, the four-helix voltage-sensing modules (VSMs) of CaV1.1 calcium channels simultaneously gate two Ca(2+) pathways: the CaV1.1 pore itself and the RyR1 calcium release channel in the sarcoplasmic reticulum. Here, to gain insight into the mechanism by which VSMs gate RyR1, we quantify intramembrane charge movement associated with VSM activation (sensing current) and gated Ca(2+) release flux in single muscle cells of mice and rats. As found for most four-helix VSMs, upon sustained depolarization, rodent VSMs lose the ability to activate Ca(2+) release channels opening; their properties change from a functionally capable mode, in which the mobile sensor charge is called charge 1, to an inactivated mode, charge 2, with a voltage dependence shifted toward more negative voltages...
November 6, 2017: Journal of General Physiology
https://www.readbyqxmd.com/read/29018092/molecular-characterization-and-verification-of-azido-3-8-dideoxy-d-manno-oct-2-ulosonic-acid-incorporation-into-bacterial-lipopolysaccharide
#15
Inga Nilsson, Kerri Grove, Dustin Dovala, Tsuyoshi Uehara, Guillaume Lapointe, David A Six
3-Deoxy-D-manno-oct-2-ulosonic acid (Kdo) is an essential component of LPS in the outer leaflet of the Gram-negative bacterial outer membrane. While labeling of Escherichia coli with the chemical reporter 8-azido-3,8-dideoxy-D-manno-oct-2-ulosonic acid (Kdo-N3) has been reported, its incorporation into LPS has not been directly shown. We have now verified Kdo-N3 incorporation into E. coli LPS at the molecular level. Using PAGE analysis, we show that Kdo-N3 is localized to the outer membrane and specifically incorporates into rough and deep-rough LPS...
October 9, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28990834/eustachian-tube-evaluation-in-patients-with-obstructive-sleep-apnea-syndrome
#16
Giuseppe Magliulo, Marco de Vincentiis, Giannicola Iannella, Andrea Ciofalo, Alessandra Manno, Benedetta Pasquariello, Diletta Angeletti, Annalisa Pace, Giampiero Gulotta, Antonella Polimeni
OBJECTIVES: Investigate the presence of Eustachian Tube Dysfunction (ETD) in Obstructive sleep apnea (OSA) patients. Correlate the ET function and the severity of the sleep apnea syndrome. Correlate nasal resistance and nasal mucociliary clearance with the onset of ETD in these patients. MATERIALS AND METHODS: Forty patients affected by OSA were enrolled in our clinical prospective study. Thirty patients were also investigated as a control group. All of the analyzed subjects underwent ENT examination, anterior rhinomanometry and test for mucociliary transport time...
October 9, 2017: Acta Oto-laryngologica
https://www.readbyqxmd.com/read/28986923/crystal-structure-of-d-glycero-%C3%AE-d-manno-heptose-1-phosphate-adenylyltransferase-from-burkholderia-pseudomallei
#17
Jimin Park, Hyojin Kim, Suwon Kim, Daeun Lee, Mi-Sun Kim, Dong Hae Shin
The crystal structure of HldC from B. pseudomallei (BpHldC), the fourth enzyme of the heptose biosynthesis pathway, has been determined. BpHldC converts ATP and d-glycero-β-d-manno-heptose-1-phosphate into ADP-d-glycero-β -d-manno-heptose and pyrophosphate. The crystal structure of BpHldC belongs to the nucleotidyltransferase α/β phosphodiesterase superfamily sharing a common Rossmann-like α/β fold with a conserved T/HXGH sequence motif. The invariant catalytic key residues of BpHldC indicate that the core catalytic mechanism of BpHldC may be similar to that of other closest homologues...
October 7, 2017: Proteins
https://www.readbyqxmd.com/read/28967810/scuba-diving-death-always-due-to-drowning-two-forensic-cases-and-a-review-of-the-literature
#18
I Aquila, F Pepe, M Manno, P Frati, S Gratteri, V Fineschi, P Ricci
Scuba diving is an increasingly common recreational activity. We describe the physiopathology of barotrauma in two cases where death was caused by pulmonary barotrauma while diving. An inspection and autopsy were carried out in both cases. The autopsy data were supported by post-mortem radiological investigation. Histological and toxicological analyses were also carried out, and dive computer and tank manometer analysis performed. In both cases, the cause of death was attributable to arterial gas embolism, resulting from pulmonary barotrauma subsequent to pulmonary over-distension...
January 1, 2017: Medico-legal Journal
https://www.readbyqxmd.com/read/28951114/italian-society-of-digestive-endoscopy-sied-position-paper-on-the-non-anaesthesiologist-administration-of-propofol-for-gastrointestinal-endoscopy
#19
Rita Conigliaro, Lorella Fanti, Mauro Manno, Piero Brosolo
Propofol sedation by non-anesthesiologists in GI endoscopy, despite generally considered a safe procedure, is still a matter of debate. Benefits of propofol sedation include rapid onset of action, greater patient comfort and fast recovery with prompt discharge from the endoscopy unit. The use of propofol for sedation in GI endoscopy, preceded by dedicated training courses, has been approved by several anaesthesiologist and gastroenterologist societies but an Italian position paper taking into account the Italian law is lacking...
September 5, 2017: Digestive and Liver Disease
https://www.readbyqxmd.com/read/28933277/identification-of-an-interaction-between-a-histidine-kinase-response-regulator-hybrid-protein-and-2-keto-3-deoxy-d-manno-octulosonate-8-phosphate-synthase-in-gluconobacter-oxydans
#20
Cuijuan Yuan, Yangchen Lin, Yushu Ma, Dongzhi Wei
The histidine kinase response regulator hybrid protein was identified as a member of two-component signaling systems in Gluconobacter oxydans by gene annotation, but the function of this protein remains unknown. In this research, its specific interaction with a 2-keto-3-deoxy-D-manno-octulosonate-8-phosphate synthase was screened by yeast two-hybrid assay. The interaction between the two proteins was further identified by GST pull-down assay and bimolecular fluorescence complementation assay in vitro and in vivo, respectively...
September 19, 2017: Protein and Peptide Letters
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