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Edward M Manno, William D Freeman, Sarah Livesay, Romergryko G Geocadin, Michel Torbey, Paul Nyquist, Claude Hemphill
No abstract text is available yet for this article.
November 2016: Critical Care Medicine
Khamphone Yelithao, Utoomporn Surayot, Ju Hun Lee, SangGuan You
Water-soluble polysaccharides isolated from the rhizome of Polygonatum sibiricum and fractionated using ion-exchange chromatography were investigated to determine their structure and immunostimulating activity. Crude and fractions (F1 and F2) consisted of carbohydrates (85.1~88.3%) with proteins (4.51~11.9%) and uronic acid (1.79~7.47%), and included different levels of mannose (62.3~76.3%), glucose (15.2~20.3%), galactose (4.35~15.3%), and arabinose (4.00~7.65%). The crude contained two peaks with molecular weights (Mw) of 151×10(3) and 31...
September 2016: Preventive Nutrition and Food Science
Alasdair Bamford, Emma C Manno, Maria Jose Mellado, Vana Spoulou, Laura Marques, Henriette J Scherpbier, Tim Niehues, Agnieszka Oldakowska, Paolo Rossi, Paolo Palma
BACKGROUND: Current national immunisation schedules differ between countries in terms of vaccine formulation, timing of vaccinations and immunisation programme funding and co-ordination. As a result, some HIV infected paediatric population may be left susceptible to vaccine preventable infections. Vaccines used in healthy population should be subjected to high quality ethical research and be explicitly validated for use in children with special vaccination needs such as those infected with HIV...
October 7, 2016: Vaccine
Gioele La Manno, Daniel Gyllborg, Simone Codeluppi, Kaneyasu Nishimura, Carmen Salto, Amit Zeisel, Lars E Borm, Simon R W Stott, Enrique M Toledo, J Carlos Villaescusa, Peter Lönnerberg, Jesper Ryge, Roger A Barker, Ernest Arenas, Sten Linnarsson
Understanding human embryonic ventral midbrain is of major interest for Parkinson's disease. However, the cell types, their gene expression dynamics, and their relationship to commonly used rodent models remain to be defined. We performed single-cell RNA sequencing to examine ventral midbrain development in human and mouse. We found 25 molecularly defined human cell types, including five subtypes of radial glia-like cells and four progenitors. In the mouse, two mature fetal dopaminergic neuron subtypes diversified into five adult classes during postnatal development...
October 6, 2016: Cell
Hoang-Minh Nguyen, Geir Mathiesen, Elena Maria Stelzer, Mai Lan Pham, Katarzyna Kuczkowska, Alasdair Mackenzie, Jane W Agger, Vincent G H Eijsink, Montarop Yamabhai, Clemens K Peterbauer, Dietmar Haltrich, Thu-Ha Nguyen
BACKGROUND: Lactobacillus plantarum is considered as a potential cell factory because of its GRAS (generally recognized as safe) status and long history of use in food applications. Its possible applications include in situ delivery of proteins to a host, based on its ability to persist at mucosal surfaces of the human intestine, and the production of food-related enzymes. By displaying different enzymes on the surface of L. plantarum cells these could be used as whole-cell biocatalysts for the production of oligosaccharides...
October 4, 2016: Microbial Cell Factories
Kurni Kurniyati, John F Kelly, Evgeny Vinogradov, Anna Robotham, Youbing Tu, Juyu Wang, Jun Liu, Susan M Logan, Chunhao Li
While protein glycosylation has been reported in several spirochetes including the syphilis bacterium Treponema pallidum and Lyme disease pathogen Borrelia burgdorferi, the pertinent glycan structures and their roles remain uncharacterized. Herein, we report a novel glycan with an unusual chemical composition and structure in the oral spirochete Treponema denticola, a keystone pathogen of periodontitis. The identified glycan of mass 450.2 Da is composed of a monoacetylated nonulosonic acid (Non) with a novel extended N7 acyl modification, a 2-methoxy-4,5,6-trihydroxy-hexanoyl residue in which the Non has a pseudaminic acid configuration (L-glycero-L-manno) and is β-linked to serine or threonine residues...
October 1, 2016: Molecular Microbiology
Verónica Luque-Agudo, Ana María González Gutiérrez, Irene Lagunes, Federico López Galindo, José M Padrón, Emilio Román, José Antonio Serrano, María Victoria Gil
Michael additions between carbohydrate derived nitroalkenes and several aliphatic and aromatic amines proceeded in a stereoselective way, leading to peracetylated 2-amino-1,2-dideoxy-1-nitro-heptitols. In addition, the antiproliferative activity of some of the new adducts has been studied. The results allowed to identify lead compounds which show GI50 values in the range 1.7-19μM.
September 23, 2016: Bioorganic Chemistry
Laura Di Manno, Jacqui A Macdonald, Tess Knight
No abstract text is available yet for this article.
November 15, 2016: Journal of Affective Disorders
G M Streshinskaya, A S Sashkov, E M Tul'skaya, S N Senchenkova, A S Dmitrenok, N F Piskunkova, O V Bueva, L I Evtushenko
The structures of cell wall glycopolymers from the type strains of three Actinoplanes species were investigated using chemical methods, NMR spectroscopy, and mass spectrometry. Actinoplanes digitatis VKM Ac-649(T) contains two phosphate-containing glycopolymers: poly(diglycosyl-1-phosphate) →6)-α-D-GlcpNAc-(1-P-6)-α-D-GlcpN-(1→ and teichoic acid →1)-sn-Gro-(3-P-3)-β-[β-D-GlcpNAc-(1→2]-D-Galp-(1→. Two glycopolymers were identified in A. auranticolor VKM Ac-648(T) and A. cyaneus VKM Ac-1095(T): minor polymer - unsubstituted 2,3-poly(glycerol phosphate), widely abundant in actinobacteria (Ac-648(T)), and mannan with trisaccharide repeating unit →2)-α-D-Manp-(1→2)-α-D-Manp-(1→6)-α-D-Manp-(1→ (Ac-1095(T))...
September 2016: Biochemistry. Biokhimii︠a︡
Benedetta Bertolotti, Beáta Oroszová, Ieva Sutkeviciute, Ladislav Kniežo, Franck Fieschi, Kamil Parkan, Zuzana Lovyová, Martina Kašáková, Jitka Moravcová
The discovery of effective ligands for DC-SIGN receptor is one of the most challenging concepts of antiviral drug design due to the importance of this C-type lectin in infection processes. DC-SIGN recognizes mannosylated and fucosylated oligosaccharides but glycosidic linkages are accessible to both chemical and enzymatic degradations. To avoid this problem, the synthesis of stable glycoside mimetics has attracted increasing attention. In this work we establish for the first time mono- and divalent C-glycosides based on d-manno and l-fuco configurations as prospective DC-SIGN ligands...
September 9, 2016: Carbohydrate Research
Simon Joost, Amit Zeisel, Tina Jacob, Xiaoyan Sun, Gioele La Manno, Peter Lönnerberg, Sten Linnarsson, Maria Kasper
The murine epidermis with its hair follicles represents an invaluable model system for tissue regeneration and stem cell research. Here we used single-cell RNA-sequencing to reveal how cellular heterogeneity of murine telogen epidermis is tuned at the transcriptional level. Unbiased clustering of 1,422 single-cell transcriptomes revealed 25 distinct populations of interfollicular and follicular epidermal cells. Our data allowed the reconstruction of gene expression programs during epidermal differentiation and along the proximal-distal axis of the hair follicle at unprecedented resolution...
September 28, 2016: Cell Systems
Salvatore Rocca, Veronica Santilli, Nicola Cotugno, Carlo Concato, Emma Concetta Manno, Giulia Nocentini, Giulia Macchiarulo, Caterina Cancrini, Andrea Finocchi, Isabella Guzzo, Luca Dello Strologo, Paolo Palma
Vaccine-preventable diseases are a significant cause of morbidity and mortality in solid organ transplant recipients who undergo immunosuppression after transplantation. Data on immune responses and long-term maintenance after vaccinations in such population are still limited.We cross-sectionally evaluated the maintenance of immune response to measles vaccine in kidney transplanted children on immunosuppressive therapy. Measles-specific enzyme-linked immunosorbent assay and B-cell enzyme-linked immunosorbent spot were performed in 74 kidney transplant patients (Tps) and in 23 healthy controls (HCs) previously vaccinated and tested for humoral protection against measles...
September 2016: Medicine (Baltimore)
Marcelina Mazur, Barbara Barycza, Hanitra Andriamboavonjy, Serge Lavoie, Marielle Tamigney Kenfack, Anaïs Laroussarie, Yves Bleriot, Charles Gauthier
3-Deoxy-beta-d-manno-oct-2-ulosonic acid (beta-Kdo) glycosides are mainly found in capsular polysaccharides (CPS) and extracellular exopolysaccharides (EPS) from Gram-negative bacteria. These compounds have profound biological implications in immune response and act as virulence factors. We have developed a novel methodology for the stereoselective synthesis of beta-Kdo glycosides via the use of a 4'-methoxyphenacyl (Phen) auxiliary group at the C1 position of a peracetylated beta-Kdo thioglycoside. Under the promotion of NIS/AgOTf in acetonitrile, a series of Kdo glycosides was synthesized in good yield and beta-selectivity while minimizing the formation of undesirable glycals...
September 14, 2016: Journal of Organic Chemistry
Rebecca E Kasper, Jonathan Green, Rachelle N Damle, Jeremy Aidlen, Pradeep Nazarey, Mariann Manno, Esther Borer, Michael P Hirsh
BACKGROUND: Gun buyback programs represent one arm of a multipronged approach to raise awareness and education about gun safety. METHODS: The city of Worcester, MA has conducted an annual gun buyback at the Police Department Headquarters since 2002. We analyzed survey responses from a voluntary, 18-question, face-to-face structured interview from December 2009 to June 2015 using descriptive statistics to determine participant demographics and motivations for participation...
August 30, 2016: Journal of Pediatric Surgery
Tim Steffens, Katarzyna Duda, Buko Lindner, Frank-Jörg Vorhölter, Hanna Bednarz, Karsten Niehaus, Otto Holst
Xanthomonas translucens pv. translucens (Xtt) is a Gram-negative pathogen of crops from the plant family Poaceae The lipopolysaccharide (LPS) of Xtt was isolated and chemically characterized. The analyses revealed the presence of rhamnose, xylose, mannose, glucose, galacturonic acid, phosphates, 3-deoxy-D-manno-oct-2-ulopyranosonic acid (Kdo) and fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0)...
September 9, 2016: Glycobiology
Giannicola Iannella, Alessandra Manno, Emanuela Pasqualitto, Andrea Ciofalo, Diletta Angeletti, Benedetta Pasquariello, Giuseppe Magliulo
Cerebrospinal fluid (CSF) leakage of the temporal bone region is defined as abnormal communications between the subarachnoidal space and the air-containing spaces of the temporal bone. CSF leak remains one of the most frequent complications after VS surgery. Radiotherapy is considered a predisposing factor for development of temporal bone CSF leak because it may impair dural repair mechanisms, thus causing inadequate dural sealing. The authors describe the case of a 47-year-old man with a massive effusion of CSF which extended from the posterior and lateral skull base to the first cervical vertebrae; this complication appeared after a partial enucleation of a vestibular schwannoma (VS) with subsequent radiation treatment and second operation with total VS resection...
2016: Case Reports in Otolaryngology
Maxim S Kokoulin, Alexandra S Kuzmich, Anatoly I Kalinovsky, Svetlana V Tomshich, Lyudmila A Romanenko, Valery V Mikhailov, Nadezhda A Komandrova
We presented the structure of the polysaccharide moiety and anticancer activity in vitro of the sulfated lipopolysaccharide isolated from the marine bacterium Cobetia litoralis KMM 3880(T). The structure of O-polysaccharide was investigated by chemical methods along with (1)H and (13)C NMR spectroscopy. The O-polysaccharide was built up of branched trisaccharide repeating units consist of D-glucose (D-Glcр), D-mannose (D-Manр) and sulfated 3-deoxy-d-manno-oct-2-ulosonic acid (Kdo5S): →7-β-Kdoр4Ac5S-(2→4)-[β-d-Glcp-(1→2)-]-β-d-Manр6Ac-1→...
December 10, 2016: Carbohydrate Polymers
Alessandro Furlan, Gioele La Manno, Moritz Lübke, Martin Häring, Hind Abdo, Hannah Hochgerner, Jussi Kupari, Dmitry Usoskin, Matti S Airaksinen, Guillermo Oliver, Sten Linnarsson, Patrik Ernfors
Despite the variety of physiological and target-related functions, little is known regarding the cellular complexity in the sympathetic ganglion. We explored the heterogeneity of mouse stellate and thoracic ganglia and found an unexpected variety of cell types. We identified specialized populations of nipple- and pilo-erector muscle neurons. These neurons extended axonal projections and were born among other neurons during embryogenesis, but remained unspecialized until target organogenesis occurred postnatally...
October 2016: Nature Neuroscience
Birgit Manno, Thomas Oellerich, Tim Schnyder, Jasmin Corso, Marion Lösing, Konstantin Neumann, Henning Urlaub, Facundo D Batista, Michael Engelke, Jürgen Wienands
The SH2 domain-containing inositol 5'-phosphatase (SHIP) plays a key role in preventing autoimmune phenomena by limiting antigen-mediated B cell activation. SHIP function is thought to require the dual engagement of the BCR and negative regulatory coreceptors as only the latter appear capable of recruiting SHIP from the cytosol to the plasma membrane by virtue of phosphorylated immunoreceptor tyrosine-based inhibitory motifs. Here we demonstrate a coreceptor-independent membrane recruitment and function of SHIP in B cells...
August 23, 2016: European Journal of Immunology
Masahito Hashimoto, Mami Ozono, Maiko Furuyashiki, Risako Baba, Shuhei Hashiguchi, Yasuo Suda, Koichi Fukase, Yukari Fujimoto
Acetobacter pasteurianus is an aerobic Gram-negative rod that is used in the fermentation process used to produce the traditional Japanese black rice vinegar kurozu. Previously, we found that a hydrophobic fraction derived from kurozu stimulates Toll-like receptors to produce cytokines. LPSs, particularly LPS from A. pasteurianus, are strong candidates for the immunostimulatory component of kurozu. The LPS of A. pasteurianus remains stable in acidic conditions during the 2 years of the abovementioned fermentation process...
September 30, 2016: Journal of Biological Chemistry
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