Tomoki Okada, Atsushi Okada, Hiromasa Aoki, Daichi Onozato, Taiki Kato, Hiroshi Takase, Shigeru Ohshima, Teruaki Sugino, Rei Unno, Kazumi Taguchi, Shuzo Hamamoto, Ryosuke Ando, Issei S Shimada, Tadahiro Hashita, Takahiro Iwao, Tamihide Matsunaga, Takahiro Yasui
Macrophages play a role in nephrolithiasis, offering the possibility of developing macrophage-mediated preventive therapies. To establish a system for screening drugs that could prevent the formation of kidney stones, we aimed to develop a model using human induced pluripotent stem cell (iPSC)-derived macrophages to study phagocytosis of calcium oxalate monohydrate (COM) crystals. Human iPSCs (201B7) were cultured. CD14+ monocytes were recovered using a stepwise process that involved the use of growth factors and cytokines...
March 30, 2024: Urolithiasis