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Małgorzata Olejnik, Piotr Jedziniak, Teresa Szprengier-Juszkiewicz
The control of levels of anticoccidial feed additives in targeted feeds plays an important role in the assurance of efficiency of animal treatment, prevention of drug resistance, and food safety. The robust and labour-efficient method for the simultaneous determination of six ionophore coccidiostats (lasalocid, maduramicin, monensin, narasin, salinomycin, and semduramicin) in targeted feed has been developed. Properly grinded and homogenized feed sample was spiked with internal standard (monesin methyl ester) and extracted with methanol...
2013: TheScientificWorldJournal
Ye Tian, Zhi-juan Lin, Ping Sun, Yan-fei Liu, Ji-yu Ju, Shu-juan Liang
AIM: To establish a murine secreted mature peptide IL-1β expression vector, transfect into Hepa1-6 hepatoma cells, and analyze the effect of recombinant IL-1β on proliferation, migration, and its specific expression in Hepa1-6 hepatoma cells. METHODS: The murine AFP signal peptide encoding sequence and mature IL-1β encoding fragment were linked together through overlapping PCR, and the chimeric DNA sequence was then inserted into a liver specific expression vector pLIVE(TM); to make a recombinant pLIVE-smIL-1β which expressed secreted murine IL-1β of classical pathway...
May 2012: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
Jae Youl Cho
Chloroquine is one of the disease-modifying antirheumatic drugs (DMARDs) with anti-malarial effect. In this study, we examined the modulatory effect of chloroquine on the functional activation of beta1-integrins (CD29) using CD29- and CD98 (a functional regulator of CD29)-mediated U937 cell-cell adhesion, comparing macrophage functions and T cell proliferation. Chloroquine effectively suppressed U937 cell-cell adhesion mediated by CD29 and CD98, in a protein kinase (PK) C, PKA, protein tyrosine kinase (PTK), extracellular signal-regulated kinase (ERK) and actin cytoskeleton-independent manner...
April 2008: Biological & Pharmaceutical Bulletin
P Melendez, J P Goff, C A Risco, L F Archbald, R Littell, G A Donovan
The objective of this study was to determine the effect of a monensin controlled-release capsule on the proportion of cows with subclinical ketosis (SK). During July to August 2001, 300 cows dried-off 50-70 days before expected parturition were randomly assigned to either a treatment (n = 150, oral capsule, 335 mg/d of monesin for 95 d) or control group (no capsule, n = 150). At 14 days postpartum, a milk sample was obtained and evaluated for beta-hydroxybutyrate (BHBA) using a semi-quantitative ketone test strip...
January 16, 2006: Preventive Veterinary Medicine
V Sureshkumar, K V Venkateswaran, S Jayasundar
Enrofloxacin, a fluoroquinolone, and its interaction with monensin, an ionophore drug, was studied to explore the influence of enrofloxacin on drug metabolizing enzymes that can lead to physiological and toxicological consequences upon coadministration with monensin in broiler chickens. Group I, treated with 100 mg monesin/kg feed from 1 d old to 41st d of age, did not show any influence on aniline hydroxylase and cytochrome b5 levels. Group II, treated with 10 mg enrofloxacin/kg body weight per os for three consecutive days on 33rd, 34th, 35th d of age, had a highly significant decrease in aniline hydroxylase on 38th d (ie on 3rd d post-treatment with enrofloxacin); a reversal effect was noticed on the 41st day (ie on 6th d post-treatment with enrofloxacin)...
October 2004: Veterinary and Human Toxicology
G Szücs, P Laczay, J Bajnógel, Z Móra
Studies were carried out to investigate the effects of monensin and tiamulin, and the simultaneous administration of both compounds on microsomal enzymes in rats. In Phase I of the experiments the effects of monensin and tiamulin were studied separately (monensin 10, 30, and 50 mg/kg or tiamulin 40, 120, and 200 mg/kg body weight, respectively), while in Phase II the two compounds were administered simultaneously (monesin 10 mg/kg and tiamulin 40 mg/kg b.w., respectively). When monensin was administered by itself, it exerted no significant effect on microsomal liver enzymes...
2000: Acta Veterinaria Hungarica
X D Wang, J G Kiang, L W Scheibel, R C Smallridge
BACKGROUND: Monensin, a Na+ ionophore, can increase cytosolic Ca2+ ([Ca2+]i) by reversing the Na+/Ca2+ exchange mechanism. This study provided additional insights into the mechanism of this Na+ ionophore-induced increase in [Ca2+]i, and emphasized the critical role of phospholipase C (PLC) in amplifying Na+/Ca2+ exchange-induced Ca2+ influx and subsequent arachidonic acid (AA) release in FRTL-5 thyroid cells. The possible involvement of protein kinase C (PKC), mitogen-activated protein kinase (MAPK), and GTP-binding (G) protein in mediating monensin-induced AA release was also explored...
September 1999: Journal of Investigative Medicine: the Official Publication of the American Federation for Clinical Research
J Strindhall, A Lundblad, P Påhlsson
The expression of E-selectin reaches a maximum 4-6 h after stimulation of human umbilical vein endothelial cells (HUVEC) in vitro with tumour necrosis factor-alpha (TNF-alpha) and then declines to basal level within 24 h. If interferon-gamma (IFN-gamma) is added to the cell culture medium together with TNF-alpha the surface expression of E-selectin is augmented and prolonged in a synergistic way. The aim of the present study was to investigate if altered protein glycosylation could explain the IFN-gamma induced persistent surface expression of E-selectin...
October 1997: Scandinavian Journal of Immunology
A Casu, L Camogliano
Glibenclamide, a second generation sulfonylurea, is an oral hypoglycemic drug. It seems to act mainly on the ATP-driven K(+)- channels of the beta-cells of pancreas determining insulin secretion. Because monensin, a Na+/H+ antiport, is able when administered to rats in vivo to inhibit insulin secretion, the action of glibenclamide is studied on glycemia and insulinemia to verify if it can antagonize the action of monensin. The results show that glibenclamide is able to partly reverse ionophore induced hyperglycemia and the inhibition of insulin secretion...
1993: Life Sciences
G W Horn, T L Mader, S L Armbruster, R R Frahm
The effects of monensin or ruminal fermentation, forage intake and weight gains of stocker cattle grazed on wheat pasture were studied. In the first of two ruminal fermentation studies, ruminal fluid pH was increased and total volatile fatty acid (VFA) concentrations at 4 (p less than .01) and 24 hr postfeeding were decreased in steers fed 200 mg monensin/day. However, no such changes in ruminal fluid pH and total VFA concentrations in monensin-fed steers were observed in trial 2. The ruminal fluid acetic to propionic acid ratio of steers that received monensin was decreased (P less than 0...
March 1981: Journal of Animal Science
H Kuwayama, T Kanazawa
Highly purified vesicles of cardiac sarcolemma were prepared from a homogenate of canine ventricular muscle by density gradient centrifugation. The preparation showed an extremely high content of (Na+,K+)-ATPase. The steady state levels of Na+-dependent phosphoenzyme formation in the presence of Triton X-100 and the specific ouabain binding in the absence of Triton X-100 were, respectively, 773 and 907 under the optimum conditions. On the other hand, the amount of Ca2+-dependent phosphoenzyme formed in the absence of Triton X--100 was less than 2 pmol...
April 1982: Journal of Biochemistry
L Y Bourguignon, B C Pressman
The carboxylic ionophore monensin has a biphasic effect on antibody-induced Thy-1 cap formation. At higher concentrations, 5 X 10(-6)-5 X 10(-5) M monesin causes a significant inhibition of receptor capping similar to that previously found with the Ca2+ selective ionophore A23187. At lower concentrations, 5 X 10(-8)-5 X 10(-7) M capping is stimulated. It is concluded that capping at lower ionophore concentrations is a specific response to the ability of monensin to induce a rise in intracellular Na+, which indirectly elevates intracellular Ca2+ activity...
1983: Journal of Membrane Biology
D G Burrin, R A Stock, R A Britton
Two finishing trials were conducted to measure the response of cattle adjusting to high-concentrate diets to dietary monensin level. In trial 1, 54 individually fed Hereford-Angus steers (312 kg), previously fed a two-thirds corn silage: one-third corncob-based diet, were allotted in a 2 X 3 factorial arrangement of treatment with tylosin (0, 11 mg/kg) and monesin (0, 11, 33 mg/kg) fed during a 28-d, grain-adaptation period (fed 75% concentrate for 6 d and then fed 95% concentrate). After 28 d, all steers were continued on their respective levels of tylosin and 33 mg/kg monensin for the remaining 119 d...
February 1988: Journal of Animal Science
F G Riddell, S Arumugam, B G Cox
Addition of monesin to preparations of large unilamellar vesicles made from egg yolk phosphatidylcholine (EPC) in sodium or potassium chloride solution and from dioleoylphosphatidylcholine (DOPC) in sodium chloride solutions gives rise to dynamic 23Na- and 39K-NMR spectra. The dynamic spectra arise from the monensin-mediated transport of the metal ions through the membrane. The kinetics of the transport are followed as a function of monensin and metal ion concentrations and are compatible with a model in which one monensin molecule transports one metal ion...
October 6, 1988: Biochimica et Biophysica Acta
A Severini, K V Prasad, A F Almeida, J G Kaplan
The early activation of Na+,K+-ATPase-mediated ion fluxes after concanavalin A (ConA) stimulation of pig lymphocytes is caused by an increase in intracellular Na+ concentration. A second mechanism of regulation of Na+,K+-ATPase activity becomes apparent between 3 and 5 h after mitogenic stimulation, but prior to onset of increase in cell volume; this consists of an increase (about 75%) in the number of ouabain-binding sites (from 35 X 10(3) +/- 12 X 10(3)/cell in resting to 60 X 10(3) +/- 27 X 10(3)/cell in activated lymphocytes)...
February 1987: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
N Masaki, A P Thomas, J B Hoek, J L Farber
Cultured rat hepatocytes were treated with potassium cyanide, an inhibitor of cytochrome oxidase; valinomycin, a K+ ionophore; carbonyl cyanide m-chlorophenylhydrazone (CCCP), a protonophore; and the ATP synthetase inhibitor oligomycin. The effect of these agents on the viability of the cells was related to changes in ATP content and the deenergization of the mitochondria. The ATP content was reduced by over 90% by each inhibitor. All of the agents except oligomycin killed the cells within 4 h. With the exception of oligomycin, the mitochondrial membrane potential as measured by the distribution of [3H]triphenylmethylphosphonium collapsed with each of the agents...
July 1989: Archives of Biochemistry and Biophysics
R J Rothman, B Perussia, D Herlyn, L Warren
Inhibitors of glycosylation and carbohydrate processing were used to probe the functional consequences of specific, differential alterations in glycosylation of monoclonal IgG secreted by hybridoma clones. Neither the absence of glycosylation nor the presence of atypical oligosaccharides significantly influenced binding of the monoclonal antibody to the cell surface antigen recognized. However, lymphocyte-mediated antibody-dependent cytotoxicity was enhanced significantly, as compared to native (unmodified) IgG-sensitized target cells, when target cells were sensitized with IgG bearing the atypical oligosaccharides induced metabolically by castanospermine, N-methyldeoxynojirimycin, deoxymannojirimycin or monesin, but not by swainsonine...
December 1989: Molecular Immunology
J Van Sande, F Deneubourg, R Beauwens, J C Braekman, D Daloze, J E Dumont
Dysidenin, a hexachlorinated tripeptide-like molecule extracted from the sponge Dysidea herbacea, has lethal effects on fishes and some marine organisms. In an in vitro screening study, this molecule appeared to be a strong inhibitor of iodide transport in dog thyroid slices. Ouabain blocks iodide transport by inhibiting the Na+/K+ ATPase, which sustains the Na+ gradient needed to drive iodide transport. Dysidenin and ouabain block iodide transport with the same kinetics but not by the same mechanisms; dysidenin, unlike ouabain, did not inhibit 86Rb+ uptake or increase its efflux...
April 1990: Molecular Pharmacology
H Masuno, E J Blanchette-Mackie, S S Chernick, R O Scow
Combined lipase deficiency (cld) is a recessive mutation which causes a severe deficiency of lipoprotein lipase and hepatic lipase activities and lethal hypertriacylglycerolemia within 3 days in newborn mice. The effect of this genetic defect on lipoprotein lipase was studied in primary cultures of brown adipocytes derived from tissue of newborn mice. Cells cultured from cld/cld mice replicated, accumulated triacylglycerol, and differentiated into adipocytes at normal rates. Lipoprotein lipase activity in unaffected cells was detectable on Day 0 of confluence and increased to 1...
January 25, 1990: Journal of Biological Chemistry
D A Jans, P Jans, H Luzius, F Fahrenholz
The ionophore monensin was found to markedly reduce the rate of return of vasopressin V2-receptors to the membrane following down-regulation with [Arg8]vasopressin (AVP), as well as hormone dissociation (unloading) from cells following ligand binding and internalization in LLC-PK1 renal epithelial cells. Monensin-resistant LLC-PK1 mutants were isolated and characterized for V2-receptor recycling. Whilst the MN-41 mutant appeared to be impaired in [3H]AVP internalization, the MN-11 and MN-21 mutants exhibited parental V2-receptor binding and internalization, but markedly impaired receptor recycling subsequent to ligand-dependent receptor down-regulation...
October 1991: Molecular and Cellular Endocrinology
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