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https://www.readbyqxmd.com/read/29906224/high-salt-intake-shifts-the-mechanisms-of-flow-induced-dilation-in-the-middle-cerebral-arteries-of-sprague-dawley-rats
#1
Anita Matic, Ivana Jukic, Ana Stupin, Lidija Baric, Zrinka Mihaljevic, Sanela Unfirer, Ivana Tartaro Bujak, Branka Mihaljevic, Julian H Lombard, Ines Drenjancevic
The goal of this study was to examine the effect of 1-week of high salt (HS) intake and the role of oxidative stress in changing the mechanisms of flow-induced dilation (FID) in isolated pressurized middle cerebral arteries (MCA) of male Sprague-Dawley rats (N=15-16/per group). Reduced FID in the HS group was restored by intake of the superoxide scavenger TEMPOL (HS+TEMPOL in vivo group). Nitric oxide synthases (NOS) inhibitor Nω -nitro-L-arginine methyl ester (L-NAME), COX inhibitor indomethacin (INDO) and selective inhibitor of microsomal CYP450 epoxidase activity N-(methylsulfonyl)-2-(2-propynyloxy)-benzenehexanamide (MS-PPOH) significantly reduced FID in the LS group, while FID in the HS group was mediated by NO only...
June 15, 2018: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/29902966/transcriptional-and-physiological-analyses-of-short-term-iron-deficiency-response-in-apple-seedlings-provide-insight-into-the-regulation-involved-in-photosynthesis
#2
Yan-Xiu Wang, Ya Hu, Yan-Fang Zhu, Abdul Wahid Baloch, Xu-Mei Jia, Ai-Xia Guo
BACKGROUND: Iron (Fe) is an essential micronutrient for plants. Utilization of Fe deficiency-tolerant rootstock is an effective strategy to prevent Fe deficiency problems in fruit trees production. Malus halliana is an apple rootstock that is resistant to Fe deficiency; however, few molecular studies have been conducted on M. halliana. RESULTS: To evaluate short-term molecular response of M. halliana leaves under Fe deficiency condition, RNA sequencing (RNA-Seq) analyses were conducted at 0 (T1), 0...
June 15, 2018: BMC Genomics
https://www.readbyqxmd.com/read/29765154/the-cancer-associated-microprotein-casimo1-controls-cell-proliferation-and-interacts-with-squalene-epoxidase-modulating-lipid-droplet-formation
#3
Maria Polycarpou-Schwarz, Matthias Groß, Pieter Mestdagh, Johanna Schott, Stefanie E Grund, Catherina Hildenbrand, Joachim Rom, Sebastian Aulmann, Hans-Peter Sinn, Jo Vandesompele, Sven Diederichs
Breast cancer is a leading cause of cancer-related death in women. Small open reading frame (sORF)-encoded proteins or microproteins constitute a new class of molecules often transcribed from presumed long non-coding RNA transcripts (lncRNAs). The translation of some of these sORFs has been confirmed, but their cellular function and importance remains largely unknown. Here, we report the identification and characterization of a novel microprotein of 10 kDa, which we named Cancer-Associated Small Integral Membrane Open reading frame 1 (CASIMO1)...
May 16, 2018: Oncogene
https://www.readbyqxmd.com/read/29693318/synthesis-activity-and-docking-studies-of-eugenol-based-glucosides-as-new-agents-against-candida-sp
#4
Taciane Maíra Magalhães Hipólito, Guilherme Tadeu Lemos Bastos, Thúlio Wliandon Lemos Barbosa, Thiago Belarmino de Souza, Luiz Felipe Leomil Coelho, Amanda Latércia Tranches Dias, Ihosvany Camps Rodríguez, Marcelo Henrique Dos Santos, Danielle Ferreira Dias, Lucas Lopardi Franco, Diogo Teixeira Carvalho
Seventeen new synthetic derivatives of eugenol (6, 8-15 and 8'-15') were planned following literature reports on antifungal activities of nitroeugenol and eugenol glucoside. The anti-Candida activity of these compounds was investigated by in vitro assay and the cytotoxicity evaluation was performed with the most active compounds. The peracetylated glucosides presented better biological results than their hydroxylated analogues. The glucoside 11, a 4-nitrobenzamide, showed the best potency (MIC50 range 11.0-151...
April 25, 2018: Chemical Biology & Drug Design
https://www.readbyqxmd.com/read/29669855/squalene-epoxidase-drives-nafld-induced-hepatocellular-carcinoma-and-is-a-pharmaceutical-target
#5
Dabin Liu, Chi Chun Wong, Li Fu, Huarong Chen, Liuyang Zhao, Chuangen Li, Yunfei Zhou, Yanquan Zhang, Weiqi Xu, Yidong Yang, Bin Wu, Gong Cheng, Paul Bo-San Lai, Nathalie Wong, Joseph J Y Sung, Jun Yu
Nonalcoholic fatty liver disease (NAFLD)-induced hepatocellular carcinoma (HCC) is an emerging malignancy in the developed world; however, mechanisms that contribute to its formation are largely unknown, and targeted therapy is currently not available. Our RNA sequencing analysis of NAFLD-HCC samples revealed squalene epoxidase ( SQLE ) as the top outlier metabolic gene overexpressed in NAFLD-HCC patients. Hepatocyte-specific Sqle transgenic expression in mice accelerated the development of high-fat, high-cholesterol diet-induced HCC...
April 18, 2018: Science Translational Medicine
https://www.readbyqxmd.com/read/29625087/the-peculiar-npq-regulation-in-the-stramenopile-phaeomonas-sp-challenges-the-xanthophyll-cycle-dogma
#6
N Berne, T Fabryova, B Istaz, P Cardol, B Bailleul
In changing light conditions, photosynthetic organisms develop different strategies to maintain a fine balance between light harvesting, photochemistry, and photoprotection. One of the most widespread photoprotective mechanisms consists in the dissipation of excess light energy in the form of heat in the photosystem II antenna, which participates to the Non Photochemical Quenching (NPQ) of chlorophyll fluorescence. It is tightly related to the reversible epoxidation of xanthophyll pigments, catalyzed by the two enzymes, the violaxanthin deepoxidase and the zeaxanthin epoxidase...
July 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29614810/vpstya1-vpstya2b-of-variovorax-paradoxus-eps-an-aryl-alkyl-sulfoxidase-rather-than-a-styrene-epoxidizing-monooxygenase
#7
Dirk Tischler, Ringo Schwabe, Lucas Siegel, Kristin Joffroy, Stefan R Kaschabek, Anika Scholtissek, Thomas Heine
Herein we describe the first representative of an E2-type two-component styrene monooxygenase of proteobacteria. It comprises a single epoxidase protein ( Vp StyA1) and a two domain protein ( Vp StyA2B) harboring an epoxidase (A2) and a FAD-reductase (B) domain. It was annotated as Vp StyA1/ Vp StyA2B of Variovorax paradoxus EPS. Vp StyA2B serves mainly as NADH:FAD-oxidoreductase. A K m of 33.6 ± 4.0 µM for FAD and a k cat of 22.3 ± 1.1 s-1 were determined and resulted in a catalytic efficiency ( k cat K m -1 ) of 0...
April 2, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29596888/squalene-epoxidase-as-a-promising-metabolic-target-in-cancer-treatment
#8
Gabriella Cirmena, Paola Franceschelli, Edoardo Isnaldi, Lorenzo Ferrando, Marilena De Mariano, Alberto Ballestrero, Gabriele Zoppoli
Oncogenic alteration of the cholesterol synthesis pathway is a recognized mechanism of metabolic adaptation. In the present review, we focus on squalene epoxidase (SE), one of the two rate-limiting enzymes in cholesterol synthesis, retracing its history since its discovery as an antimycotic target to its description as an emerging metabolic oncogene by amplification with clinical relevance in cancer. We review the published literature assessing the association between SE over-expression and poor prognosis in this disease...
July 1, 2018: Cancer Letters
https://www.readbyqxmd.com/read/29577447/high-terbinafine-resistance-in-trichophyton-interdigitale-isolates-in-delhi-india-harbouring-mutations-in-the-squalene-epoxidase-gene
#9
Ashutosh Singh, Aradhana Masih, Ananta Khurana, Pradeep Kumar Singh, Meenakshi Gupta, Ferry Hagen, Jacques F Meis, Anuradha Chowdhary
In the last few years, infections caused by dermatophytes along with a concomitant increase in the number of difficult to treat cases have increasingly been recognised, indicating that dermatophytosis remains a challenging public health problem. The majority of infections are caused by Trichophyton rubrum and Trichophyton mentagrophytes complex. Terbinafine, an allylamine antifungal used orally and topically is considered to be a first-line drug in the therapy of dermatophyte infections. Terbinafine resistance has been predominately attributed to point mutations in the squalene epoxidase (SQLE) target gene a key enzyme in the ergosterol biosynthetic pathway leading to single amino acid substitutions...
March 25, 2018: Mycoses
https://www.readbyqxmd.com/read/29551944/medically-important-carotenoids-from-momordica-charantia-and-their-gene-expressions-in-different-organs
#10
Do Manh Cuong, Mariadhas Valan Arasu, Jin Jeon, Yun Ji Park, Soon-Jae Kwon, Naif Abdullah Al-Dhabi, Sang Un Park
Carotenoids, found in the fruit and different organs of bitter melon ( Momordica charantia ), have attracted great attention for their potential health benefits in treating several major chronic diseases. Therefore, study related to the identification and quantification of the medically important carotenoid metabolites is highly important for the treatment of various disorderes. The present study involved in the identification and quantification of the various carotenoids present in the different organs of M...
December 2017: Saudi Journal of Biological Sciences
https://www.readbyqxmd.com/read/29530857/mutation-in-the-squalene-epoxidase-gene-of-trichophyton-interdigitale-and-trichophyton-rubrum-associated-with-allylamine-resistance
#11
Shivaprakash M Rudramurthy, Shamanth A Shankarnarayan, Sunil Dogra, Dipika Shaw, Khurram Mushtaq, Raees A Paul, Tarun Narang, Arunaloke Chakrabarti
Dermatophytosis, the commonest superficial fungal infection, has gained recent attention due to its change of epidemiology and treatment failures. Despite the availability of several agents effective against dermatophytes, the incidences of chronic infection, reinfection, and treatment failures are on the rise. Trichophyton rubrum and Trichophyton interdigitale are the two species most frequently identified among clinical isolates in India. Consecutive patients ( n = 195) with suspected dermatophytosis during the second half of 2014 were included in this study...
May 2018: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29510567/phenolic-compounds-from-belamcanda-chinensis-seeds
#12
Ying-Ying Song, Ying Liu, Yong-Ming Yan, Xi-Feng Lu, Yong-Xian Cheng
Two new sucrose derivatives, namely, belamcanosides A ( 1 ) and B ( 2 ), together with five other known compounds ( 3 - 7 ), were isolated from the seeds of Belamcanda chinensis (L.) DC. Their structures were identified based on spectroscopic data. Especially, the absolute configurations of fructose and glucose residues in 1 and 2 were assigned by acid hydrolysis, followed by derivatization and gas chromatography (GC) analysis. Among the known compounds, (-)-hopeaphenol ( 3 ), (+)-syringaresinol ( 4 ), and quercetin ( 5 ), were isolated from B...
March 5, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29501490/physiological-validation-of-photochemical-reflectance-index-pri-as-a-photosynthetic-parameter-using-arabidopsis-thaliana-mutants
#13
Kaori Kohzuma, Kouki Hikosaka
Non-photochemical quenching (NPQ) is the most important photoprotective system in higher plants. NPQ can be divided into several steps according to the timescale of relaxation of chlorophyll fluorescence after reaching a steady state (i.e., the fast phase, qE; middle phase, qZ or qT; and slow phase, qI). The dissipation of excess energy as heat during the xanthophyll cycle, a large component of NPQ, is detectable during the fast to middle phase (sec to min). Although thermal dissipation is primarily investigated using indirect methods such as chlorophyll a fluorescence measurements, such analyses require dark adaptation or the application of a saturating pulse during measurement, making it difficult to continuously monitor this process...
March 25, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29396745/high-level-recombinant-production-of-squalene-using-selected-saccharomyces-cerevisiae-strains
#14
Jong Yun Han, Sung Hwa Seo, Jae Myeong Song, Hongweon Lee, Eui-Sung Choi
For recombinant production of squalene, which is a triterpenoid compound with increasing industrial applications, in microorganisms generally recognized as safe, we screened Saccharomyces cerevisiae strains to determine their suitability. A strong strain dependence was observed in squalene productivity among Saccharomyces cerevisiae strains upon overexpression of genes important for isoprenoid biosynthesis. In particular, a high level of squalene production (400 ± 45 mg/L) was obtained in shake flasks with the Y2805 strain overexpressing genes encoding a bacterial farnesyl diphosphate synthase (ispA) and a truncated form of hydroxyl-3-methylglutaryl-CoA reductase (tHMG1)...
April 2018: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/29378090/a-chimeric-styrene-monooxygenase-with-increased-efficiency-in-asymmetric-biocatalytic-epoxidation
#15
Maria L Corrado, Tanja Knaus, Francesco G Mutti
The styrene monooxygenase (SMO) system from Pseudomonas sp. consists of two enzymes (StyA and StyB). StyB catalyses the reduction of FAD at the expense of NADH. After the transfer of FADH2 from StyB to StyA, reaction with O2 generates FAD-OOH, which is the epoxidising agent. The wastage of redox equivalents due to partial diffusive transfer of FADH2 , the insolubility of recombinant StyB and the impossibility of expressing StyA and StyB in a 1:1 molar ratio reduce the catalytic efficiency of the natural system...
April 4, 2018: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/29345751/first-evidence-of-freezing-tolerance-in-a-resurrection-plant-insights-into-molecular-mobility-and-zeaxanthin-synthesis-in-the-dark
#16
Beatriz Fernández-Marín, Gilbert Neuner, Edith Kuprian, Jose Manuel Laza, J I José Ignacio García-Plazaola, Amy Verhoeven
The photoprotective mechanisms of desiccation tolerance and freezing tolerance and their relation to molecular mobility (cell vitrification) were assessed in a single model: the exceptional subalpine and resurrection plant Ramonda myconi. Dehydrated leaves showed a drop in Fv /Fm accompanied by synthesis of zeaxanthin (Z), even in the dark, which was limited by cell vitrification after complete desiccation. The recovery of Fv /Fm after a severe drying treatment (7 d at 50% RH) confirmed the tolerance of R. myconi leaves to desiccation...
January 18, 2018: Physiologia Plantarum
https://www.readbyqxmd.com/read/29283570/heterologous-biosynthesis-of-nodulisporic-acid-f
#17
Kyle C Van de Bittner, Matthew J Nicholson, Leyla Y Bustamante, Sarah A Kessans, Arvina Ram, Craig J van Dolleweerd, Barry Scott, Emily J Parker
Nodulisporic acids comprise a group of valuable indole diterpenes that exhibit potent insecticidal activities. We report the identification of a gene cluster in the genome of the filamentous fungus Hypoxylon pulicicidum (Nodulisporium sp.) that contains genes responsible for the biosynthesis of nodulisporic acids. Using Penicillium paxilli as a heterologous host, and through pathway reconstitution experiments, we identified the function of four genes involved in the biosynthesis of the nodulisporic acid core compound, nodulisporic acid F (NAF)...
January 17, 2018: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/29203557/a-single-oxidosqualene-cyclase-produces-the-seco-triterpenoid-%C3%AE-onocerin
#18
Aldo Almeida, Lemeng Dong, Bekzod Khakimov, Jean-Etienne Bassard, Tessa Moses, Frederic Lota, Alain Goossens, Giovanni Appendino, Søren Bak
8,14- seco -Triterpenoids are characterized by their unusual open C-ring. Their distribution in nature is rare and scattered in taxonomically unrelated plants. The 8,14- seco -triterpenoid α-onocerin is only known from the evolutionarily distant clubmoss genus Lycopodium and the leguminous genus Ononis , which makes the biosynthesis of this seco -triterpenoid intriguing from an evolutionary standpoint. In our experiments with Ononis spinosa, α-onocerin was detected only in the roots. Through transcriptome analysis of the roots, an oxidosqualene cyclase, OsONS1, was identified that produces α-onocerin from squalene-2,3;22,23-dioxide when transiently expressed in Nicotiana bethamiana In contrast, in Lycopodium clavatum , two sequential cyclases, LcLCC and LcLCD, are required to produce α-onocerin in the N...
February 2018: Plant Physiology
https://www.readbyqxmd.com/read/29196684/squalene-epoxidase-plays-a-critical-role-in-determining-pig-meat-quality-by-regulating-adipogenesis-myogenesis-and-ros-scavengers
#19
Jeongim Ha, Seulgi Kwon, Jung Hye Hwang, Da Hye Park, Tae Wan Kim, Deok Gyeong Kang, Go Eun Yu, Hwa Chun Park, Sang Mi An, Chul Wook Kim
In mammals, Squalene epoxidase (SQLE) is an enzyme that converts squalene to 2,3-oxidosqualene, in the early stage of cholesterol generation. Here, we identified single nucleotide polymorphisms (SNPs) in the SQLE gene (c.2565 G > T) by RNA Sequencing from the liver tissue of Berkshire pigs. Furthermore, we found that homozygous GG pigs expressed more SQLE mRNA than GT heterozygous and TT homozygous pigs in longissimus dorsi tissue. Next, we showed that the SNP in the SQLE gene was associated with several meat quality traits including backfat thickness, carcass weight, meat colour (yellowness), fat composition, and water-holding capacity...
December 1, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29150930/photoautotrophic-production-of-macular-pigment-in-a-chlamydomonas-reinhardtii-strain-generated-by-using-dna-free-crispr-cas9-rnp-mediated-mutagenesis
#20
Kwangryul Baek, Jihyeon Yu, Jooyeon Jeong, Sang Jun Sim, Sangsu Bae, EonSeon Jin
Lutein and zeaxanthin are dietary carotenoids reported to be protective against age-related macular degeneration. Recently, the green alga Chlamydomonas reinhardtii has received attention as a photosynthetic cell factory, but the potential of this alga for carotenoid production has not yet been evaluated. In this study, we selected the C. reinhardtii CC-4349 strain as the best candidate among seven laboratory strains tested for carotenoid production. A knock-out mutant of the zeaxanthin epoxidase gene induced by preassembled DNA-free CRISPR-Cas9 ribonucleoproteins in the CC-4349 strain had a significantly higher zeaxanthin content (56-fold) and productivity (47-fold) than the wild type without the reduction in lutein level...
March 2018: Biotechnology and Bioengineering
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