Anaïs Chretien, Marius F Nagel, Sabine Botha, Raphaël de Wijn, Lea Brings, Katerina Dörner, Huijong Han, Jayanath C P Koliyadu, Romain Letrun, Adam Round, Tokushi Sato, Christina Schmidt, Radu-Costin Secareanu, David von Stetten, Mohammad Vakili, Agnieszka Wrona, Richard Bean, Adrian Mancuso, Joachim Schulz, Arwen R Pearson, Tilman Kottke, Kristina Lorenzen, Robin Schubert
The understanding of signal transduction mechanisms in photoreceptor proteins is essential for elucidating how living organisms respond to light as environmental stimuli. In this study, we investigated the ATP binding, photoactivation and signal transduction process in the photoactivatable adenylate cyclase from Oscillatoria acuminata (OaPAC) upon blue light excitation. Structural models with ATP bound in the active site of native OaPAC at cryogenic as well as room temperature are presented. ATP is found in one conformation at cryogenic- and in two conformations at ambient-temperature, and is bound in an energetically unfavorable conformation for the conversion to cAMP...
January 5, 2024: Journal of Molecular Biology