keyword
MENU ▼
Read by QxMD icon Read
search

Fat trap

keyword
https://www.readbyqxmd.com/read/28078077/a-novel-method-for-the-quantification-of-fatty-infiltration-in-skeletal-muscle
#1
Nicole K Biltz, Gretchen A Meyer
BACKGROUND: Fatty infiltration of the skeletal muscle is a common but poorly understood feature of many myopathies. It is best described in human muscle, where non-invasive imaging techniques and representative histology have been optimized to view and quantify infiltrating fat. However, human studies are limited in their ability to identify cellular and molecular mechanisms regulating fatty infiltration, a likely prerequisite to developing targeted interventions. As mechanistic investigations move to small animals, studies may benefit from new or adapted imaging tools optimized for high resolution and whole muscle quantification...
2017: Skeletal Muscle
https://www.readbyqxmd.com/read/28073372/a-novel-method-for-the-quantification-of-fatty-infiltration-in-skeletal-muscle
#2
Nicole K Biltz, Gretchen A Meyer
BACKGROUND: Fatty infiltration of the skeletal muscle is a common but poorly understood feature of many myopathies. It is best described in human muscle, where non-invasive imaging techniques and representative histology have been optimized to view and quantify infiltrating fat. However, human studies are limited in their ability to identify cellular and molecular mechanisms regulating fatty infiltration, a likely prerequisite to developing targeted interventions. As mechanistic investigations move to small animals, studies may benefit from new or adapted imaging tools optimized for high resolution and whole muscle quantification...
January 10, 2017: Skeletal Muscle
https://www.readbyqxmd.com/read/28071704/high-fat-diet-induced-changes-of-mouse-hepatic-transcription-and-enhancer-activity-can-be-reversed-by-subsequent-weight-loss
#3
Majken Siersbæk, Lyuba Varticovski, Shutong Yang, Songjoon Baek, Ronni Nielsen, Susanne Mandrup, Gordon L Hager, Jay H Chung, Lars Grøntved
Epigenetic factors have been suggested to play an important role in metabolic memory by trapping and maintaining initial metabolic changes within the transcriptional regulatory machinery. In this study we fed mice a high fat diet (HFD) for seven weeks followed by additional five weeks of chow, to identify HFD-mediated changes to the hepatic transcriptional program that may persist after weight loss. Mice fed a HFD displayed increased fasting insulin levels, hepatosteatosis and major changes in hepatic gene transcription associated with modulation of H3K27Ac at enhancers, but no significant changes in chromatin accessibility, indicating that HFD-regulated gene transcription is primarily controlled by modulating the activity of pre-established enhancers...
January 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/27992512/68ga-trap-rgd-3-hybrid-imaging-for-the-in-vivo-monitoring-of-%C3%AE-v%C3%A3-3-integrin-expression-as-biomarker-of-anti-angiogenic-therapy-effects-in-experimental-breast-cancer
#4
Philipp M Kazmierczak, Andrei Todica, Franz-Josef Gildehaus, Heidrun Hirner-Eppeneder, Matthias Brendel, Ralf S Eschbach, Magdalena Hellmann, Konstantin Nikolaou, Maximilian F Reiser, Hans-Jürgen Wester, Saskia Kropf, Axel Rominger, Clemens C Cyran
OBJECTIVES: To investigate 68Ga-TRAP-(RGD)3 hybrid imaging for the in vivo monitoring of αvß3-integrin expression as biomarker of anti-angiogenic therapy effects in experimental breast cancer. MATERIALS AND METHODS: Human breast cancer (MDA-MB-231) xenografts were implanted orthotopically into the mammary fat pads of n = 25 SCID mice. Transmission/emission scans (53 min to 90 min after i.v. injection of 20 MBq 68Ga-TRAP-(RGD)3) were performed on a dedicated small animal PET before (day 0, baseline) and after (day 7, follow-up) a 1-week therapy with the VEGF antibody bevacizumab or placebo (imaging cohort n = 13; therapy n = 7, control n = 6)...
2016: PloS One
https://www.readbyqxmd.com/read/27981392/histological-evidence-that-metformin-reverses-the-adverse-effects-of-diabetes-on-orthodontic-tooth-movement-in-rats
#5
Jing Sun, Juan Du, Wei Feng, Boyao Lu, Hongrui Liu, Jie Guo, Norio Amizuka, Minqi Li
This study evaluated the effects of metformin on orthodontic tooth movement in a rat model of type 2 diabetes mellitus. Rats were fed a high-fat diet for 4 weeks to induce fat accumulation and insulin resistance, and then injected with a low dose of streptozotocin (35 mg/kg) intraperitoneally to induce type 2 diabetes. An orthodontic appliance was placed in normoglycemic, type 2 diabetes, and type 2 diabetes with metformin-administrated rats. After 14 days, type 2 diabetes rats exhibited greater orthodontic tooth movement and had a higher number of tartrate-resistant acid phosphatase-positive osteoclasts, stronger cathepsin K expression, and weaker alkaline phosphatase immunostaining than normoglycemic rats...
December 15, 2016: Journal of Molecular Histology
https://www.readbyqxmd.com/read/27933990/automated-enrichment-of-sulfanilamide-in-milk-matrices-by-utilization-of-aptamer-linked-magnetic-particles
#6
Christin Fischer, Constanze Kallinich, Sven Klockmann, Jil Schrader, Markus Fischer
The present work demonstrates the first automated enrichment approach for antibiotics in milk using specific DNA aptamers. First, aptamers toward the antibiotic sulfanilamide were selected and characterized regarding their dissociation constants and specificity toward relevant antibiotics via fluorescence assay and LC-MS/MS detection. The performed enrichment was automated using the KingFisherDuo and compared to a manual approach. Verifying the functionality, trapping was realized in different milk matrices: (i) 0...
December 7, 2016: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/27923100/effects-of-air-pollution-exposure-on-glucose-metabolism-in-los-angeles-minority-children
#7
C M Toledo-Corral, T L Alderete, R Habre, K Berhane, F W Lurmann, M J Weigensberg, M I Goran, F D Gilliland
OBJECTIVES: Growing evidence indicates that ambient (AAP: NO2 , PM2.5 and O3 ) and traffic-related air pollutants (TRAP) contribute to metabolic disease risk in adults; however, few studies have examined these relationships in children. METHODS: Metabolic profiling was performed in 429 overweight and obese African-American and Latino youth living in urban Los Angeles, California. This cross-sectional study estimated individual residential air pollution exposure and used linear regression to examine relationships between air pollution and metabolic outcomes...
December 6, 2016: Pediatric Obesity
https://www.readbyqxmd.com/read/27768123/milk-fat-globule-egf-factor-8-suppresses-the-aberrant-immune-response-of-systemic-lupus-erythematosus-derived-neutrophils-and-associated-tissue-damage
#8
Wei Huang, Jiyuan Wu, Huiqin Yang, Yin Xiong, Rui Jiang, Tianpen Cui, Duyun Ye
Abnormal features of the systemic lupus erythematosus (SLE)-derived neutrophils, promoted aberrant immune response, have inspired new studies of the induction of autoimmunity and the development of organ damage in SLE. In this study, we explore the effect of milk fat globule-EGF factor 8 (MFG-E8) on the aberrant nitrification features in pristane-induced lupus. SLE patients and mice with pristane-induced lupus develop autoantibodies associated with MFG-E8 overproduction. However, the deletion of MFG-E8 leads to uncontrolled early pulmonary and peritoneal inflammation and tissue damage in mice with pristane-induced lupus...
October 21, 2016: Cell Death and Differentiation
https://www.readbyqxmd.com/read/27701440/inhibition-of-net-release-fails-to-reduce-adipose-tissue-inflammation-in-mice
#9
Quinte Braster, Carlos Silvestre Roig, Helene Hartwig, Linda Beckers, Myrthe den Toom, Yvonne Döring, Mat J Daemen, Esther Lutgens, Oliver Soehnlein
Obesity-associated diseases such as Type 2 diabetes, liver disease and cardiovascular diseases are profoundly mediated by low-grade chronic inflammation of the adipose tissue. Recently, the importance of neutrophils and neutrophil-derived myeloperoxidase and neutrophil elastase on the induction of insulin resistance has been established. Since neutrophil elastase and myeloperoxidase are critically involved in the release of neutrophil extracellular traps (NETs), we here hypothesized that NETs may be relevant to early adipose tissue inflammation...
2016: PloS One
https://www.readbyqxmd.com/read/27669658/reduced-femoral-bone-mass-in-both-diet-induced-and-genetic-hyperlipidemia-mice
#10
Xiang Chen, Chunyu Wang, Kun Zhang, Ying Xie, Xiao Ji, Hui Huang, Xijie Yu
Growing evidence argues for a relationship between lipid and bone metabolisms with inconsistent conclusions. Sphingosine-1-phosphate (S1P) has been recognized as a suitable candidate for possible link between lipid metabolism and bone metabolism. This study was designed to investigate the effects of hyperlipidemia on bone metabolism using diet-induced and genetic-induced hyperlipidemia animal models and to explore whether S1P is involved. Wild-type mice and low-density lipoprotein receptor gene deficient (LDLR(-/-)) mice at age of 8weeks were placed on either control diet or high-fat diet (HFD) for 12weeks...
September 23, 2016: Bone
https://www.readbyqxmd.com/read/27666759/ligand-trap-of-the-activin-receptor-type-iia-inhibits-osteoclast-stimulation-of-bone-remodeling-in-diabetic-mice-with-chronic-kidney-disease
#11
Toshifumi Sugatani, Olga A Agapova, Yifu Fang, Alycia G Berman, Joseph M Wallace, Hartmut H Malluche, Marie-Claude Faugere, William Smith, Victoria Sung, Keith A Hruska
Dysregulation of skeletal remodeling is a component of renal osteodystrophy. Previously, we showed that activin receptor signaling is differentially affected in various tissues in chronic kidney disease (CKD). We tested whether a ligand trap for the activin receptor type 2A (RAP-011) is an effective treatment of the osteodystrophy of the CKD-mineral bone disorder. With a 70% reduction in the glomerular filtration rate, CKD was induced at 14 weeks of age in the ldlr-/- high fat-fed mouse model of atherosclerotic vascular calcification and diabetes...
January 2017: Kidney International
https://www.readbyqxmd.com/read/27623367/thermophilic-and-hyper-thermophilic-co-digestion-of-waste-activated-sludge-and-fat-oil-and-grease-evaluating-and-modeling-methane-production
#12
Rania Mona Alqaralleh, Kevin Kennedy, Robert Delatolla, Majid Sartaj
Renewable energy and clean environment are two crucial requirements for our modern world. Low cost, energy production and limited environmental impact make anaerobic digestion (AD) a promising technology for stabilizing organic waste and in particular, sewage waste. The anaerobic co-digestion of thickened waste activated sludge (TWAS) and sewage treatment plant trapped fat, oil and grease (FOG) using different FOG-TWAS mixtures (20, 40, 60 and 80% of FOG based on total volatile solids (TVS)) were investigated in this study using both thermophilic (55 ± 1 °C) and two stages hyper-thermophilic/thermophilic (70 ± 1 °C and 55 ± 1 °C) anaerobic co-digestion...
December 1, 2016: Journal of Environmental Management
https://www.readbyqxmd.com/read/27531997/effect-of-high-fat-diet-on-the-formation-of-pulmonary-neutrophil-extracellular-traps-during-influenza-pneumonia-in-balb-c-mice
#13
Anandi Narayana Moorthy, Kong Bing Tan, Shi Wang, Teluguakula Narasaraju, Vincent T Chow
Obesity is an independent risk factor for severe outcome of influenza infection. Higher dietary fat consumption has been linked to greater morbidity and severe influenza in mouse models. However, the extent of generation of neutrophil extracellular traps (NETs or NETosis) in obese individuals during influenza pneumonia is hitherto unknown. This study investigated pulmonary NETs generation in BALB/c mice fed with high-fat diet (HFD) and low-fat diet (LFD), during the course of influenza pneumonia. Clinical disease progression, histopathology, lung reactive oxygen species, and myeloperoxidase activity were also compared...
2016: Frontiers in Immunology
https://www.readbyqxmd.com/read/27499624/endothelial-glycocalyx-conditions-influence-nanoparticle-uptake-for-passive-targeting
#14
Ming J Cheng, Rajiv Kumar, Srinivas Sridhar, Thomas J Webster, Eno E Ebong
Cardiovascular diseases are facilitated by endothelial cell (EC) dysfunction and coincide with EC glycocalyx coat shedding. These diseases may be prevented by delivering medications to affected vascular regions using circulating nanoparticle (NP) drug carriers. The objective of the present study was to observe how the delivery of 10 nm polyethylene glycol-coated gold NPs (PEG-AuNP) to ECs is impacted by glycocalyx structure on the EC surface. Rat fat pad endothelial cells were chosen for their robust glycocalyx, verified by fluorescent immunolabeling of adsorbed albumin and integrated heparan sulfate (HS) chains...
2016: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/27376007/effect-of-proteolytic-activities-in-combination-with-the-pectolytic-activities-on-extractability-of-the-fat-and-phenolic-compounds-from-olives
#15
Youssef Moustakime, Zakaria Hazzoumi, Khalid Amrani Joutei
During the extraction, a portion of oil remains trapped inside the cells and its release requires the degradation of the walls and cell membranes, especially when the fruits have not reached a maximum maturity which is likely to cause an optimal embrittlement of the parietal structures and cell membrane. This can be done by specific enzymes necessary for the degradation of various cellular barriers. Three different enzyme treatments proteolytic, pectolytic or both are applied on the Moroccan Picholine olives from veraison to maturity of the fruit...
2016: SpringerPlus
https://www.readbyqxmd.com/read/27373163/nucleic-acid-targeting-pathways-promote-inflammation-in-obesity-related-insulin-resistance
#16
Xavier S Revelo, Magar Ghazarian, Melissa Hui Yen Chng, Helen Luck, Justin H Kim, Kejing Zeng, Sally Y Shi, Sue Tsai, Helena Lei, Justin Kenkel, Chih Long Liu, Stephanie Tangsombatvisit, Hubert Tsui, Corneliu Sima, Changting Xiao, Lei Shen, Xiaoying Li, Tianru Jin, Gary F Lewis, Minna Woo, Paul J Utz, Michael Glogauer, Edgar Engleman, Shawn Winer, Daniel A Winer
Obesity-related inflammation of metabolic tissues, including visceral adipose tissue (VAT) and liver, are key factors in the development of insulin resistance (IR), though many of the contributing mechanisms remain unclear. We show that nucleic-acid-targeting pathways downstream of extracellular trap (ET) formation, unmethylated CpG DNA, or ribonucleic acids drive inflammation in IR. High-fat diet (HFD)-fed mice show increased release of ETs in VAT, decreased systemic clearance of ETs, and increased autoantibodies against conserved nuclear antigens...
July 19, 2016: Cell Reports
https://www.readbyqxmd.com/read/27364313/effects-of-25-hydroxycholecalciferol-supplementation-in-maternal-diets-on-milk-quality-and-serum-bone-status-markers-of-sows-and-bone-quality-of-piglets
#17
Hui Zhou, Yuling Chen, Yong Zhuo, Gang Lv, Yan Lin, Bin Feng, Zhengfeng Fang, Lianqiang Che, Jian Li, Shengyu Xu, De Wu
Twenty primiparous sows were allocated to two treatments to evaluate the effects of maternal 25-hydroxycholecalciferol (25OHD3 ) supplementation during gestation and lactation on milk quality and serum bone status markers of sows and bone quality of piglets. Immediately after mating, sows were randomly allotted to one of two diets supplemented with 50 µg/kg 25OHD3 or basal diets without 25OHD3 . Blood and milk samples were obtained. At birth and weaning, 10 piglets from each treatment were killed for bone quality analysis...
July 1, 2016: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/27339288/acute-effects-of-dietary-fatty-acids-on-osteclastogenesis-via-rankl-rank-opg-system
#18
M Carmen Naranjo, Indara Garcia, Beatriz Bermudez, Sergio Lopez, Magdalena P Cardelo, Rocio Abia, Francisco J G Muriana, Sergio Montserrat-de la Paz
SCOPE: Postprandial state is directly linked with chronic diseases. We hypothesized that dietary fats may have acute effects on health status by modulating osteoclast differentiation and activation in a fatty acid-dependent manner. METHODS AND RESULTS: In healthy subjects, a fat-enriched meal increased plasma levels of the RANKL (receptor activator of nuclear factor κB ligand)/OPG (osteoprotegerin) ratio (SFAs > MUFAs = PUFAs) in the postprandial state. Postprandial TRL-SFAs enhanced tartrate-resistant acid phosphatase (TRAP) activity and the expression of osteoclast marker genes (TRAP, OSCAR, RANK, and CATHK) while downregulated the expression of OPG gene in human monocyte-derived osteoclasts...
June 24, 2016: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/27334272/editing-transgenic-dna-components-by-inducible-gene-replacement-in-drosophila-melanogaster
#19
Chun-Chieh Lin, Christopher J Potter
Gene conversions occur when genomic double-strand DNA breaks (DSBs) trigger unidirectional transfer of genetic material from a homologous template sequence. Exogenous or mutated sequence can be introduced through this homology-directed repair (HDR). We leveraged gene conversion to develop a method for genomic editing of existing transgenic insertions in Drosophila melanogaster The clustered regularly-interspaced palindromic repeats (CRISPR)/Cas9 system is used in the H: omology A: ssisted C: RISPR K: nock-in (HACK) method to induce DSBs in a GAL4 transgene, which is repaired by a single-genomic transgenic construct containing GAL4 homologous sequences flanking a T2A-QF2 cassette...
August 2016: Genetics
https://www.readbyqxmd.com/read/27073835/spatial-distribution-of-the-metabolically-active-microbiota-within-italian-pdo-ewes-milk-cheeses
#20
Ilaria De Pasquale, Raffaella Di Cagno, Solange Buchin, Maria De Angelis, Marco Gobbetti
Italian PDO (Protected Designation of Origin) Fiore Sardo (FS), Pecorino Siciliano (PS) and Pecorino Toscano (PT) ewes' milk cheeses were chosen as hard cheese model systems to investigate the spatial distribution of the metabolically active microbiota and the related effects on proteolysis and synthesis of volatile components (VOC). Cheese slices were divided in nine sub-blocks, each one separately subjected to analysis and compared to whole cheese slice (control). Gradients for moisture, and concentrations of salt, fat and protein distinguished sub-blocks, while the cell density of the main microbial groups did not differ...
2016: PloS One
keyword
keyword
101670
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"