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https://www.readbyqxmd.com/read/29721090/harmine-enhances-type-h-vessel-formation-and-prevents-bone-loss-in-ovariectomized-mice
#1
Jie Huang, Hao Yin, Shan-Shan Rao, Ping-Li Xie, Xu Cao, Tai Rao, Shu-Ying Liu, Zhen-Xing Wang, Jia Cao, Yin Hu, Yan Zhang, Juan Luo, Yi-Juan Tan, Zheng-Zhao Liu, Ben Wu, Xiong-Ke Hu, Tuan-Hui Chen, Chun-Yuan Chen, Hui Xie
Recently, researchers identified a distinct vessel subtype called type H vessels that couple angiogenesis and osteogenesis. We previously found that type H vessels are reduced in ovariectomy (OVX)-induced osteoporotic mice, and preosteoclasts are able to secrete platelet-derived growth factor-BB (PDGF-BB) to stimulate type H vessel formation and thereby to promote osteogenesis. This study aimed to explore whether harmine, a β-carboline alkaloid, is capable of preventing bone loss in OVX mice by promoting preosteoclast PDGF-BB-induced type H vessel formation...
2018: Theranostics
https://www.readbyqxmd.com/read/29717278/role-of-obesity-in-the-release-of-extracellular-nucleosomes-in-acute-pancreatitis-a-clinical-and-experimental-study
#2
Salvador Pérez, Isabela Finamor, Pablo Martí-Andrés, Javier Pereda, Ana Campos, Rosário Domingues, Fawaz Haj, Luis Sabater, Enrique de-Madaria, Juan Sastre
BACKGROUND/OBJECTIVES: A high body mass index increases the risk of severe pancreatitis and associated mortality. Our aims were: (1) To determine whether obesity affects the release of extracellular nucleosomes in patients with pancreatitis; (2) To determine whether pancreatic ascites confers lipotoxicity and triggers the release of extracellular nucleosomes in lean and obese rats. METHODS: DNA and nucleosomes were determined in plasma from patients with mild or moderately severe acute pancreatitis either with normal or high body mass index (BMI)...
May 1, 2018: International Journal of Obesity: Journal of the International Association for the Study of Obesity
https://www.readbyqxmd.com/read/29668869/multistate-comparison-of-attractants-and-the-impact-of-fruit-development-stage-on-trapping-drosophila-suzukii-diptera-drosophilidae-in-raspberry-and-blueberry
#3
Benjamin D Jaffe, Alina Avanesyan, Harit K Bal, Yan Feng, Joshua Grant, Matthew J Grieshop, Jana C Lee, Oscar E Liburd, Elena Rhodes, Cesar Rodriguez-Saona, Ashfaq A Sial, Aijun Zhang, Christelle Guédot
Spotted-wing drosophila, Drosophila suzukii Matsumura (Diptera: Drosophilidae), is an invasive pest of soft-skinned fruits across the globe. Effective monitoring is necessary to manage this pest, but suitable attractants are still being identified. In this study, we combined lures with fermenting liquid baits to improve D. suzukii trapping specificity and attractiveness. We also measured the efficiency and specificity of baits/lures during different times of the season; the reproductive status of females among baits/lures; and the effects of locations and crop type on these response variables...
April 14, 2018: Environmental Entomology
https://www.readbyqxmd.com/read/29644703/evaluation-of-six-sample-preparation-procedures-for-qualitative-and-quantitative-proteomics-analysis-of-milk-fat-globule-membrane
#4
Yongxin Yang, Elizabeth Anderson, Sheng Zhang
Proteomic analysis of membrane proteins is challenged by the proteins solubility and detergent incompatibility with MS analysis. No single perfect protocol can be used to comprehensively characterize the proteome of membrane fraction. Here, we used cow milk fat globule membrane (MFGM) proteome analysis to assess six sample preparation procedures including one in-gel and five in-solution digestion approaches prior to LC-MS/MS analysis. The largest number of MFGM proteins were identified by suspension trapping (S-Trap) and filter-aided sample preparation (FASP) methods, followed by acetone precipitation without clean-up of tryptic peptides method...
April 12, 2018: Electrophoresis
https://www.readbyqxmd.com/read/29630153/successful-management-of-congenital-infantile-fibrosarcoma-presenting-as-large-non-healing-buttock-ulceration
#5
Madeleine G Sowash, Karen Itumeleng Mosojane, Alan R Anderson, Carrie L Kovari, Victoria L Williams
A two-year-old boy presented with a large, non-healing ulceration on his left buttock, which was originally noted as a brown patch present at birth. Punch skin biopsy was performed and histopathology revealed an atypical, pleomorphic, spindled proliferation in whorled fascicles replacing the dermis and trapping fat in the subcutis, consistent with a diagnosis of congenital/infantile fibrosarcoma. No evidence of metastatic spread was seen on imaging. The tumor was initially deemed unresectable owing to extent of local invasion...
February 15, 2018: Dermatology Online Journal
https://www.readbyqxmd.com/read/29624834/selective-imaging-of-lipids-in-adipocytes-using-an-imidazolyl-derivative-of-perylenebisimide
#6
Jeyaraman Sankar, Ruchika Mishra, Zeeshan Mushtaq, Bhagaban Mallik, Ramprasad Regar, Vimlesh Kumar
A small molecule PBI-ID has been identified and developed as a specific marker for labeling multifunctional fat bodies in various organisms including Drosophila and mammalian adipocytes. Interestingly, PBI-ID neither labels the plasma membranes nor the cell nuclei by trapping into it. A remarkable feature of unbound PBI-ID is the diminished fluorescence, which reduces the background emission noise while contrasting the bound state effectively.
April 6, 2018: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/29525729/immunophenotypic-analysis-of-the-chronological-events-of-tissue-repair-in-aortic-medial-dissections
#7
Silvia D Visonà, Onno J de Boer, Claire Mackaaij, Hans H de Boer, Kartika R Pertiwi, Ruben W de Winter, Antonio Osculati, Allard C van der Wal
Acute medial dissection of aorta can occur in the context of a sudden and unexpected death. For medico-legal reasons it is important to estimate as accurately the histological age of dissections. We evaluated the additional value of a systematic application of immunohistochemistry, compared with conventional histology only, in determining chronological steps of injury and repair. Thirty two paraffin embedded specimens of aortic dissection were retrospectively allocated to one of four defined stages: acute (I), subacute (II), early organizing (III) and scarring (IV) using Hematoxylin and Eosin and Elastica van Gieson stained sections...
May 2018: Cardiovascular Pathology: the Official Journal of the Society for Cardiovascular Pathology
https://www.readbyqxmd.com/read/29501334/evaluation-of-a-commercial-vacuum-fly-trap-for-controlling-flies-on-organic-dairy-farms
#8
M J Kienitz, B J Heins, R D Moon
The objective of this study was to evaluate the efficacy of a commercial vacuum fly trap (CowVac, Spalding Laboratories, Reno, NV) in on-farm organic dairy production systems to control horn flies, stable flies, and face flies. As cows walk through the trap, flies are brushed off the face, flank, and back with hanging flaps and blown off the belly, udder, and legs from one side, and then vacuumed from the air into a chamber from vacuum inlets opposite the blower and above the cow. The study included 8 organic dairy farms during the summer of 2015 in Minnesota, and herds ranged from 30 to 350 cows in size...
May 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/29455287/ionic-liquid-phases-with-comprehensive-two-dimensional-gas-chromatography-of-fatty-acid-methyl-esters
#9
Siriluck Pojjanapornpun, Yada Nolvachai, Kornkanok Aryusuk, Chadin Kulsing, Kanit Krisnangkura, Philip J Marriott
New generation inert ionic liquid (iIL) GC columns IL60i, IL76i and IL111i, comprising phosphonium or imidazolium cationic species, were investigated for separation of fatty acid methyl esters (FAME). In general, the iIL phases provide comparable retention times to their corresponding conventional columns, with only minor selectivity differences. The average tailing factors and peak widths were noticeably improved (reduced) for IL60i and IL76i, while they were slightly improved for IL111i. Inert IL phase columns were coupled with conventional IL columns in comprehensive two-dimensional GC (GC × GC) with a solid-state modulator which offers variable modulation temperature (TM ), programmable TM during analysis and trapping stationary phase material during the trap/release (modulation) process, independent of oven T and column sets...
February 17, 2018: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29433264/dairy-curd-coagulated-by-a-plant-extract-of-calotropis-procera-role-of-fat-structure-on-the-chemical-and-textural-characteristics
#10
Rayanatou Issa Ado, Christelle Lopez, Valérie Lechevalier, Mahamadou Elhadji Gounga, Benoit Robert, Marielle Harel-Oger, Gilles Garric, Jean-François Grongnet, Frédéric Gaucheron
Milk is often subjected to technological treatments which have impacts on the structure of milk constituents and the characteristics of rennet curds. In this paper, the influence of the dairy fat structure on the biochemical and textural characteristics of curds coagulated by an extract of Calotropis procera leaves was studied. Standardized milks were reconstituted with the same contents in protein (35g·kg-1 ) and fat (35g·kg-1 ) but with different structures of fat i.e. homogenized anhydrous milk fat (HAMF), homogenized cream (HC) and non-homogenized cream (NHC)...
March 2018: Food Research International
https://www.readbyqxmd.com/read/29390724/super-alfv%C3%A3-nic-translation-of-a-field-reversed-configuration-into-a-large-bore-dielectric-chamber
#11
J Sekiguchi, T Asai, T Takahashi
An experimental device to demonstrate additional heating and control methods for a field-reversed configuration (FRC) has been developed. The newly developed device, named FRC Amplification via Translation (FAT), has a field-reversed theta-pinch plasma source and a low-elongation dielectric (transparent quartz) confinement chamber with quasi-static confinement field. In the initial experiments on the FAT device, FRC translation and trapping were successfully demonstrated. Although the typical elongation of the trapped FRC in the confinement region was roughly three, no disruptive global instability, such as tilt, was observed...
January 2018: Review of Scientific Instruments
https://www.readbyqxmd.com/read/29324774/activated-carbon-n-acetylcysteine-microcapsule-protects-against-nonalcoholic-fatty-liver-disease-in-young-rats-via-activating-telomerase-and-inhibiting-apoptosis
#12
Tingting Shi, Xingxin Yang, Hongping Zhou, Jianjun Xi, Jingjing Sun, Yunling Ke, Jiankang Zhang, Yidan Shao, Xiaojie Jiang, Xuwang Pan, Shourong Liu, Rangxiao Zhuang
Non-alcoholic fatty liver disease (NAFLD) is becoming one of the world's most common chronic liver diseases in childhood, yet no therapy is available that has been approved by the food and drug administration (FDA). Previous studies have reported that telomere and telomerase are involved the development and progression of NAFLD. This study was designed to investigate the potential beneficial effects of activated carbon N-acetylcysteine (ACNAC) microcapsules on the development of NAFLD in young rats as well as the underlying mechanism(s) involved...
2018: PloS One
https://www.readbyqxmd.com/read/29287318/how-ingredients-influence-furan-and-aroma-generation-in-sponge-cake
#13
Mayela Cepeda-Vázquez, Barbara Rega, Nicolas Descharles, Valérie Camel
A wide range of compounds can be formed during thermal processing of food, some of which are relevant for aroma (e.g., furfural), while others are of great health concern (e.g., furan). This paper presents the study of formulation as affecting the simultaneous generation of furan and furfural, along with other aroma quality markers, in sponge cake by means of headspace trap/GC-MS. Ingredients were screened according to their category (fat, salt, sugar, egg-based). Glucose-containing formulation resulted in the highest content of furan and furfural (12...
April 15, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29274064/metabolic-and-bone-effects-of-high-fat-diet-in-adult-zebrafish
#14
Marta Carnovali, Livio Luzi, Ileana Terruzzi, Giuseppe Banfi, Massimo Mariotti
An increase of visceral fat affects human bone health causing fragility, mechanical strength reduction, and increased propensity to fractures because of impaired bone matrix microstructure and aberrant bone cell function. Adult Danio rerio (zebrafish) represents a powerful model to study both metabolic diseases and bone metabolism. The aim of this study was to generate an obese adult zebrafish by high-fat diet and evaluate metabolic and bone tissue effects. Fish blood glucose and insulin levels were found to be altered in high-fat diet fish revealing a failure in β-cells insulin production...
December 22, 2017: Endocrine
https://www.readbyqxmd.com/read/29220800/exposure-to-traffic-related-air-pollution-and-the-composition-of-the-gut-microbiota-in-overweight-and-obese-adolescents
#15
Tanya L Alderete, Roshonda B Jones, Zhanghua Chen, Jeniffer S Kim, Rima Habre, Frederick Lurmann, Frank D Gilliland, Michael I Goran
BACKGROUND: Traffic-related air pollution (TRAP) exposure has been linked to type 2 diabetes and metabolic dysfunction in humans. Animal studies suggest that air pollutants may alter the composition of the gut microbiota, which may negatively impact metabolic health through changes in the composition and/or function of the gut microbiome. OBJECTIVES: The primary aim of this study was to determine whether elevated TRAP exposure was correlated with gut bacterial taxa in overweight and obese adolescents from the Meta-AIR (Metabolic and Asthma Incidence Research) study...
February 2018: Environmental Research
https://www.readbyqxmd.com/read/29197561/phase-angle-is-related-with-inflammatory-and-oxidative-stress-biomarkers-in-older-women
#16
Crisieli Maria Tomeleri, Cláudia Regina Cavaglieri, Mariana Ferreira de Souza, Edilaine Fungari Cavalcante, Melissa Antunes, Hellen Clair Garcez Nabbuco, Danielle Venturini, Decio Sabbatini Barbosa, Analiza Mônica Silva, Edilson Serpeloni Cyrino
BACKGROUND: The aim of this study was to examine the relation between phase angle (PhA) and inflammatory and oxidative stress biomarkers in older women. METHODS: One hundred and fifty-five physically independent older women participated in this study (67.7±5.7years, 27.0±4.4kg/m2 ). Inflammatory markers included interleukin 6 (IL-6), tumor necrosis factor alpha (TNF-α), and acute phase reactive protein (CRP). Oxidative stress biomarkers comprised superoxide dismutase (SOD), catalase (CAT), advanced oxidation protein products (AOPP), and total radical-trapping antioxidant potential (TRAP)...
February 2018: Experimental Gerontology
https://www.readbyqxmd.com/read/29159892/reliability-of-body-weight-scalars-on-the-assessment-of-propofol-induction-dose-in-obese-patients
#17
A M Araújo, H S Machado, A C Falcão, P Soares-da-Silva
BACKGROUND: Obese patients require specific perioperative care when compared with non-obese patients. The present study aimed to analyse the ability of size descriptors to estimate propofol induction dose in class II and III obese patients. METHODS: A cross-sectional study on adult patients with body mass index (BMI) equal to or greater than 35 kg/m2 and on adult patients with BMI lower than 35 kg/m2 was carried out. General anaesthesia was induced with remifentanil, propofol and rocuronium...
April 2018: Acta Anaesthesiologica Scandinavica
https://www.readbyqxmd.com/read/29115514/polygonum-multiflorm-alleviates-glucocorticoid%C3%A2-induced-osteoporosis-and-wnt-signaling-pathway
#18
Manru Zhou, Jingkai Wu, Yongjie Yu, Yajun Yang, Jin Li, Liao Cui, Weimin Yao, Yuyu Liu
It is known that long‑term excessive administration of glucocorticoid (GC) results in osteoporosis. The present study aimed to evaluate the protective effects of Polygonum multiflorm (PM) on the bone tissue of rats with GC‑induced osteoporosis (GIO). A total of 90 6‑month‑old female Sprague Dawley rats (weight range, 190‑210 g) were randomly divided into nine groups: Control (normal saline); prednisone (GC; 6 mg·kg‑1·d‑1; Model); GC plus PMR30 (the 30% ethanol eluent fraction of PM) (H) (400 mg·kg‑1·d‑1); GC plus PMR30 (M) (200 mg·kg‑1·d‑1); GC plus PMR30 (L) (100 mg·kg‑1·d‑1); GC plus PMRF (fat‑soluble fraction of PM) (H) (400 mg·kg‑1·d‑1); GC plus PMRF (M) (200 mg·kg‑1·d‑1); GC plus PMRF (L) (100 mg·kg‑1·d‑1); GC plus calcitriol (CAL; 0...
January 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29018651/a-case-of-vertebral-artery-fusiform-aneurysm-treated-by-flow-alteration-successful-prediction-of-therapeutic-effects-using-computational-fluid-dynamics
#19
Yoichi Miura, Fujimaro Ishida, Yusuke Kamei, Masanori Tsuji, Masato Shiba, Hiroshi Tanemura, Yasuyuki Umeda, Shinichi Shimosaka, Hidenori Suzuki
The treatment of intracranial complicated aneurysms remains challenging. In patients with complicated aneurysms that are neither clippable nor coilable, flow alteration treatment (FAT) with a combined procedure of proximal/distal occlusion or trapping of an aneurysm with bypass surgery has been reported. However, it is difficult to predict whatever FAT can achieve aneurysmal obliteration without ischemic complications. A 69-year-old female was incidentally diagnosed with a left vertebral artery (VA) fusiform aneurysm distal to the left posterior inferior cerebellar artery (PICA)...
October 2017: NMC Case Report Journal
https://www.readbyqxmd.com/read/28986295/optimization-and-evaluation-of-zein-nanoparticles-to-improve-the-oral-delivery-of-glibenclamide-in-vivo-study-using-c-elegans
#20
David Lucio, María Cristina Martínez-Ohárriz, Gonzalo Jaras, Paula Aranaz, Carlos J González-Navarro, Aurel Radulescu, Juan M Irache
The aim of this work was to evaluate the capability of zein nanoparticles as oral carriers for glibenclamide (GB). Nanoparticles were prepared by a desolvation procedure in the presence of lysine as stabilizer. A central composite design was used to optimize this preparative process. Under the selected conditions, nanoparticles displayed a size of about 190 nm, a surface charge of -37 mV and a payload of 45 µg GB/mg. Small-angle neutron scattering and X-ray diffraction techniques suggested an internal fractal-like structure, based on the repetition of spherical blocks of zein units (about 20 nm) grouped to form the nanoparticles...
October 3, 2017: European Journal of Pharmaceutics and Biopharmaceutics
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