keyword
MENU ▼
Read by QxMD icon Read
search

psorosis

keyword
https://www.readbyqxmd.com/read/27891561/citrus-psorosis-virus-coat-protein-derived-hairpin-construct-confers-stable-transgenic-resistance-in-citrus-against-psorosis-a-and-b-syndromes
#1
A De Francesco, N Costa, M L García
Citrus psorosis virus (CPsV) is the causal agent of psorosis, a serious and widespread citrus disease. Two syndromes of psorosis, PsA and PsB, have been described. PsB is the most aggressive and rampant form. Previously, we obtained Pineapple sweet orange plants transformed with a hairpin construct derived from the CPsV coat protein gene (ihpCP). Some of these plants were resistant to CPsV 90-1-1, a PsA isolate homologous to the transgene. In this study, we found that expression of the ihpCP transgene and siRNA production in lines ihpCP-10 and -15 were stable with time and propagation...
November 28, 2016: Transgenic Research
https://www.readbyqxmd.com/read/27596536/bioinformatic-and-mutational-analysis-of-ophiovirus-movement-proteins-belonging-to-the-30k-superfamily
#2
María Belén Borniego, David Karlin, Eduardo José Peña, Gabriel Robles Luna, María Laura García
Ophioviridae is a family of segmented, negative-sense, single-stranded RNA plant viruses. We showed that their cell-to-cell movement protein (MP) is an isolated member of the 30K MP superfamily with a unique structural organization. All 30K MPs share a core domain that contains a nearly-invariant signature aspartate. We examined its role in the MP of Citrus psorosis virus (CPsV) and Mirafiori lettuce big-vein virus (MiLBVV). Alanine substitution of this aspartate prevented plasmodesmata accumulation of MP(MiLBVV), while MP(CPsV) was not affected...
November 2016: Virology
https://www.readbyqxmd.com/read/26033697/citrus-psorosis-virus-24k-protein-interacts-with-citrus-mirna-precursors-affects-their-processing-and-subsequent-mirna-accumulation-and-target-expression
#3
Carina A Reyes, Eliana E Ocolotobiche, Facundo E Marmisollé, Gabriel Robles Luna, María B Borniego, Ariel A Bazzini, Sebastian Asurmendi, María L García
Sweet orange (Citrus sinensis), one of the most important fruit crops worldwide, may suffer from disease symptoms induced by virus infections, thus resulting in dramatic economic losses. Here, we show that the infection of sweet orange plants with two isolates of Citrus psorosis virus (CPsV) expressing different symptomatology alters the accumulation of a set of endogenous microRNAs (miRNAs). Within these miRNAs, miR156, miR167 and miR171 were the most down-regulated, with almost a three-fold reduction in infected samples...
April 2016: Molecular Plant Pathology
https://www.readbyqxmd.com/read/25907469/development-and-validation-of-a-multiplex-reverse-transcription-quantitative-pcr-rt-qpcr-assay-for-the-rapid-detection-of-citrus-tristeza-virus-citrus-psorosis-virus-and-citrus-leaf-blotch-virus
#4
Fatima Osman, Emir Hodzic, Sun-Jung Kwon, Jinbo Wang, Georgios Vidalakis
A single real-time multiplex reverse transcription quantitative polymerase chain reaction (RT-qPCR) assay for the simultaneous detection of Citrus tristeza virus (CTV), Citrus psorosis virus (CPsV), and Citrus leaf blotch virus (CLBV) was developed and validated using three different fluorescently labeled minor groove binding qPCR probes. To increase the detection reliability, coat protein (CP) genes from large number of different isolates of CTV, CPsV and CLBV were sequenced and a multiple sequence alignment was generated with corresponding CP sequences from the GenBank and a robust multiplex RT-qPCR assay was designed...
August 2015: Journal of Virological Methods
https://www.readbyqxmd.com/read/25674593/recent-advances-in-citrus-psorosis-virus
#5
REVIEW
Asmae Achachi, Essaïd Ait Barka, Mohammed Ibriz
Psorosis is a globally devastating disease of citrus caused by an infectious filamentous ophiovirus, Citrus psorosis virus (CPsV), which causes annual losses of about 5 % and a progressive decline of trees by affecting the conductive tissues. The disease can be harboured asymptomatically in many citrus species. In the field, the most characteristic symptoms of the disease in adult trees are bark scaling in the trunk and main branches and also internal staining in the underlying wood. The virus has a tripartite single-stranded RNA genome, and has been inadvertently spread to most citrus growing areas through the movement of citrus propagative material...
2014: Virusdisease
https://www.readbyqxmd.com/read/24874194/a-new-ophiovirus-is-associated-with-blueberry-mosaic-disease
#6
Thanuja Thekke-Veetil, Thien Ho, Karen E Keller, Robert R Martin, Ioannis E Tzanetakis
Blueberry mosaic disease (BMD) was first described more than 60 years ago and is caused by a yet unidentified graft transmissible agent. A combination of traditional methods and next generation sequencing disclosed the presence of a new ophiovirus in symptomatic plants. The virus was detected in all BMD samples collected from several production areas of North America and was thus named blueberry mosaic associated virus. Phylogenetic analysis, supported by high bootstrap values, places the virus within the family Ophioviridae...
August 30, 2014: Virus Research
https://www.readbyqxmd.com/read/23602594/ophioviruses-cpsv-and-milbvv-movement-protein-is-encoded-in-rna-2-and-interacts-with-the-coat-protein
#7
Gabriel Robles Luna, Eduardo José Peña, María Belén Borniego, Manfred Heinlein, Maria Laura Garcia
Citrus psorosis virus (CPsV) and Mirafiori lettuce big-vein virus (MiLBVV), members of the Ophioviridae family, have segmented negative-sense single-stranded RNA genomes. To date no reports have described how ophioviruses spread within host plants and/or the proteins involved in this process. Here we show that the 54K protein of CPsV is encoded by RNA 2 and describe its subcellular distribution. Upon transient expression in Nicotiana benthamiana epidermal cells the 54K protein, and also its 54K counterpart protein of MiLBVV, localize to plasmodesmata and enhance GFP cell-to-cell diffusion between cells...
July 5, 2013: Virology
https://www.readbyqxmd.com/read/22921760/citrus-psorosis-and-mirafiori-lettuce-big-vein-ophiovirus-coat-proteins-localize-to-the-cytoplasm-and-self-interact-in-vivo
#8
Eduardo José Peña, Gabriel Robles Luna, María Cecilia Zanek, María Belén Borniego, Carina Andrea Reyes, Manfred Heinlein, María Laura García
Citrus psorosis (CPsV) and Mirafiori lettuce big-vein virus (MiLBVV) belong to the family Ophioviridae, plant viruses with filamentous nucleocapsids and segmented genomes of negative polarity, causing the worldwide distributed citrus psorosis and lettuce big-vein diseases, respectively. To gain insight into the replication cycle of these viruses, the subcellular localization of the viral coat proteins (CP) was studied. Immunoblot analysis of fractionated extracts derived from natural and experimental infected hosts indicated that the CP of CPsV occurs in the soluble cytoplasmic fraction...
December 2012: Virus Research
https://www.readbyqxmd.com/read/21084056/resistance-to-citrus-psorosis-virus-in-transgenic-sweet-orange-plants-is-triggered-by-coat-protein-rna-silencing
#9
Carina Andrea Reyes, Agustina De Francesco, Eduardo José Peña, Norma Costa, María Inés Plata, Lorena Sendin, Atilio Pedro Castagnaro, María Laura García
The lack of naturally occurring resistance to Citrus psorosis virus (CPsV) has demanded exploitation of a transgenic approach for the development of CPsV-resistant sweet orange plants. Transgenic sweet orange plants producing intron-hairpin RNA transcripts (ihpRNA) corresponding to viral cp, 54K or 24K genes were generated and analyzed at the molecular and phenotypic levels. Two independent CPsV challenge assays demonstrated that expression of ihpRNA derived from the cp gene (ihpCP) provided a high level of virus resistance, while those derived from 54K and 24K genes (ihp54K and ihp24K) provided partial or no resistance...
January 10, 2011: Journal of Biotechnology
https://www.readbyqxmd.com/read/20438136/comparison-of-ftir-spectra-between-huanglongbing-citrus-greening-and-other-citrus-maladies
#10
COMPARATIVE STUDY
Samantha A Hawkins, Bosoon Park, Gavin H Poole, Tim R Gottwald, William R Windham, Joseph Albano, Kurt C Lawrence
Fourier transform infrared (FTIR) spectroscopy has the ability to quickly identify the presence of specific carbohydrates in plant materials. The presence of the disease huanglongbing (HLB) in the leaves of infected citrus plants has a distinctive spectrum that can be used to distinguish an infected plant from a healthy plant. However, many citrus diseases display similar visible symptoms and are of concern to citrus growers. In this study several citrus diseases (citrus leaf rugose virus, citrus tristeza virus, citrus psorosis virus, and Xanthomonas axonopodis ) and nutrient deficiencies (iron, copper, zinc, manganese, and magnesium) were compared with HLB using FTIR spectroscopy to determine if the spectra alone can be used to identify plants that are infected with HLB instead of another disease...
May 26, 2010: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/20286309/the-use-of-leaf-tissue-in-graft-transmission-of-psorosis-virus
#11
J M WALLACE
No abstract text is available yet for this article.
March 1947: Phytopathology
https://www.readbyqxmd.com/read/19820946/differential-resistance-to-citrus-psorosis-virus-in-transgenic-nicotiana-benthamiana-plants-expressing-hairpin-rna-derived-from-the-coat-protein-and-54k-protein-genes
#12
Carina Andrea Reyes, Eduardo José Peña, María Cecilia Zanek, Daniela Verónica Sanchez, Oscar Grau, María Laura García
Citrus psorosis virus (CPsV), genus Ophiovirus, family Ophioviridae, is the causal agent of a serious disease affecting citrus trees in many countries. The viral genome consists of three ssRNAs of negative polarity. Post-transcriptional gene silencing (PTGS), a mechanism of plant defence against viruses, can be induced by transgenic expression of virus-derived sequences encoding hairpin RNAs. Since the production of transgenic citrus lines and their evaluation would take years, a herbaceous model plant, Nicotiana benthamiana, was used to test hairpin constructs...
December 2009: Plant Cell Reports
https://www.readbyqxmd.com/read/19226765/isolation-and-identification-of-citrus-psorosis-virus-egyptian-isolate-cpsv-eg
#13
S A Ghazal, Kh A El-Dougdoug, A A Mousa, H Fahmy, A R Sofy
Citrus psorosis ophiovirus (CPsV), is considered to be of the most serious and deter mental virus pathogen's citrus species trees in Egypt. CPsV-EG was isolated from infected citrus grapefruit (C. paradisi Macf.) at Agric. Res. Centre (ARC). The grapefruit which used for CPsV-EG isolate was found to be free from CTV, CEVd and Spiroplasma citri where as gave -ve results with DTBIA, tissue print hybridization and Diene's stain respectively. CPsV-EG was detected on the basis of biological indexing by graft inoculation which gave oak leaf pattern (OLP) on Dweet tangor and serological assay by DAS-ELISA using Mab specific CPsV...
2008: Communications in Agricultural and Applied Biological Sciences
https://www.readbyqxmd.com/read/17712560/genetic-transformation-of-sweet-orange-with-the-coat-protein-gene-of-citrus-psorosis-virus-and-evaluation-of-resistance-against-the-virus
#14
María Cecilia Zanek, Carina Andrea Reyes, Magdalena Cervera, Eduardo José Peña, Karelia Velázquez, Norma Costa, Maria Inés Plata, Oscar Grau, Leandro Peña, María Laura García
Citrus psorosis is a serious viral disease affecting citrus trees in many countries. Its causal agent is Citrus psorosis virus (CPsV), the type member of genus Ophiovirus. CPsV infects most important citrus varieties, including oranges, mandarins and grapefruits, as well as hybrids and citrus relatives used as rootstocks. Certification programs have not been sufficient to control the disease and no sources of natural resistance have been found. Pathogen-derived resistance (PDR) can provide an efficient alternative to control viral diseases in their hosts...
January 2008: Plant Cell Reports
https://www.readbyqxmd.com/read/16963769/genetic-variation-of-populations-of-citrus-psorosis-virus
#15
Susana Martín, María Laura García, Antonella Troisi, Luis Rubio, Gonzalo Legarreta, Oscar Grau, Daniela Alioto, Pedro Moreno, José Guerri
Citrus psorosis virus (CPsV), the type species of genus Ophiovirus, has a segmented, negative-stranded RNA genome. We examined the population structure and genetic variation of CPsV in three coding regions located in RNAs 1, 2 and 3, analysing 22 isolates from Argentina, California, Florida, Italy and Spain. Most isolates contained a predominant sequence and some minor variants. Estimations of the genetic diversity and phylogenetic clustering of isolates disclosed two populations, one comprising isolates from Spain, Italy, Florida and California and the other including the Argentinean isolates...
October 2006: Journal of General Virology
https://www.readbyqxmd.com/read/16872685/detection-of-citrus-psorosis-virus-in-the-northwestern-citrus-production-area-of-argentina-by-using-an-improved-tas-elisa
#16
Maria Cecilia Zanek, Eduardo Peña, Carina Andrea Reyes, Julia Figueroa, Beatriz Stein, Oscar Grau, Maria Laura Garcia
Citrus Psorosis in Argentina is a serious disease. Citrus is produced in two regions located in the northeast (NE) and northwest (NW) area of the country. These two areas have different climates and soil types, and therefore different citrus species and varieties are cultivated. In the NE region, Psorosis is epidemic, and in the NW region, the disease was described on several occasions since 1938, but it is not observed commonly in the orchards. Recently, trees with symptoms of Psorosis were observed in the Tucumán and Salta Provinces located in the NW region...
November 2006: Journal of Virological Methods
https://www.readbyqxmd.com/read/15951030/a-multiplex-polymerase-chain-reaction-method-for-reliable-sensitive-and-simultaneous-detection-of-multiple-viruses-in-citrus-trees
#17
Avijit Roy, Amer Fayad, G Barthe, R H Brlansky
A multiplex polymerase chain reaction (mPCR) assay was developed to detect six RNA and one DNA citrus virus: Citrus leaf rugose virus (CLRV), Citrus psorosis virus (CPsV), Citrus tatter leaf virus (CTLV), Citrus tristeza virus (CTV), Citrus variegation virus (CVV), Citrus yellow mosaic virus (CYMV), and Indian citrus ringspot virus (ICRSV) from citrus plants. These seven viruses are classified in six different virus genera. Degenerate primers were designed based on the respective virus isolate sequence data available from the GenBank and were used for reliable detection of the different viruses by simplex- and mPCR...
October 2005: Journal of Virological Methods
https://www.readbyqxmd.com/read/15449144/the-complete-nucleotide-sequence-of-a-spanish-isolate-of-citrus-psorosis-virus-comparative-analysis-with-other-ophioviruses
#18
S Martín, C López, M L García, G Naum-Onganía, O Grau, R Flores, P Moreno, J Guerri
The complete genomic sequence (11278 nt) of Citrus psorosis virus (CPsV), isolate P-121 from Spain, was determined and compared with those from isolate CPV-4 and from other ophioviruses. The three RNAs of P-121 had similar size and identical organization as those of CPV-4. The 24K and the RdRp proteins were potentially encoded in the viral complementary (vc) strand of RNA 1, the 54K protein potentially encoded in vcRNA 2 and the coat protein encoded in vcRNA 3. These four proteins from P-121 and CPV-4 had 87, 92, 93 and 94% amino acid identity, respectively, but only 22, 38, 25 and 33% identity with their homologous proteins from Mirafiori lettuce big vein virus (MLBVV), the only other ophiovirus completely sequenced...
January 2005: Archives of Virology
https://www.readbyqxmd.com/read/14579175/variability-of-the-coat-protein-gene-of-citrus-psorosis-virus-in-campania-southern-italy
#19
D Alioto, M Malfitano, A Troisi, A Peluso, S Martin, R G Milne, J Guerri, P Moreno
Variability of the Coat protein (CP) gene of Citrus psorosis virus (CPsV) was assessed serologically, and by sequence analyses of two genomic regions located in the 3' (region C) and 5' (region V) halves of the gene. Analysis of 53 psorosis field sources from Campania, Italy, with 23 monoclonal antibodies revealed nine serogroups and at least ten different epitopes. Sequence analysis of 19 of these sources showed limited nucleotide diversity of the CP gene in the population. Diversity was slightly higher in region V than in region C...
November 2003: Archives of Virology
https://www.readbyqxmd.com/read/13885728/serological-investigations-involving-the-infectious-variegation-strain-of-psorosis-virus-of-citrus
#20
P R DESJARDINS, J M WALLACE
No abstract text is available yet for this article.
January 1962: Virology
keyword
keyword
101654
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"