keyword
https://read.qxmd.com/read/30644435/genetically-diverse-plasmodium-falciparum-infections-within-host-competition-and-symptomatic-malaria-in-humans
#21
JOURNAL ARTICLE
Paul Sondo, Karim Derra, Thierry Lefevre, Seydou Diallo-Nakanabo, Zekiba Tarnagda, Odile Zampa, Adama Kazienga, Innocent Valea, Hermann Sorgho, Jean-Bosco Ouedraogo, Tinga Robert Guiguemde, Halidou Tinto
There is a large genetic diversity of Plasmodium falciparum strains that infect people causing diverse malaria symptoms. This study was carried out to explore the effect of mixed-strain infections and the extent to which some specific P. falciparum variants are associated with particular malaria symptoms. P. falciparum isolates collected during pharmacovigilance study in Nanoro, Burkina Faso were used to determine allelic variation in two polymorphic antigens of the merozoite surface (msp1 and msp2). Overall, parasite density did not increase with additional strains, suggesting the existence of within-host competition...
January 15, 2019: Scientific Reports
https://read.qxmd.com/read/30558627/characterization-of-plasmodium-falciparum-structure-in-nigeria-with-malaria-snps-barcode
#22
JOURNAL ARTICLE
Bolajoko E Bankole, Adeyemi T Kayode, Iguosadolo O Nosamiefan, Philomena Eromon, Mary L Baniecki, Rachel F Daniels, Elizabeth J Hamilton, Katelyn Durfee, Bronwyn MacInnis, Henrietta Okafor, Akintunde Sowunmi, Sarah K Volkman, Pardis Sabeti, Dyann Wirth, Christian T Happi, Onikepe A Folarin
BACKGROUND: Plasmodium falciparum malaria remains a major health challenge in Nigeria despite the global decline of its incidence and mortality rates. Although significant progress has been made in preventing the transmission of P. falciparum and controlling the spread of the infection, there is much to be done in the area of proper monitoring, surveillance of the parasite, investigating the population dynamics and drug resistance profiling of the parasite as these are important to its eventual eradication...
December 17, 2018: Malaria Journal
https://read.qxmd.com/read/29973679/identifying-recrudescent-plasmodium-falciparum-in-treated-malaria-patients-by-real-time-pcr-and-high-resolution-melt-analysis-of-genetic-diversity
#23
JOURNAL ARTICLE
Khalid B Beshir, Nouhoum Diallo, Colin J Sutherland
Recurrent parasitaemia during follow up of clinical trials of antimalarial drug efficacy results from either recrudescence of parasites surviving treatment or from parasites newly emerging from the hepatic stage of infection. Nested PCR is used to distinguish these two possibilities and the technique is difficult to standardise. There is risk of both false positive and false negative results, leading to misclassification errors. The high-resolution melt (HRM) assay was developed with pairs of conserved primers targeting blocks of merozoite surface protein 1 and 2 (msp1 and msp2) genes, and polymorphisms were compared using sequence-confirmed Plasmodium falciparum DNA samples from laboratory isolates...
July 4, 2018: Scientific Reports
https://read.qxmd.com/read/28835262/natural-antibody-responses-to-plasmodium-falciparum-msp3-and-glurp-r0-antigens-are-associated-with-low-parasite-densities-in-malaria-patients-living-in-the-central-region-of-ghana
#24
JOURNAL ARTICLE
L E Amoah, S V Nuvor, E K Obboh, F K Acquah, K Asare, S K Singh, J N Boampong, M Theisen, K C Williamson
BACKGROUND: Plasmodium falciparum genetic diversity and multiplicity of infection (MOI) are parasite features that have been suggested to influence the acquisition of protective immunity against malaria. This study sought to assess the relationship between MOI and parasite density (PD) in malaria patients living in the Central Region of Ghana and to determine whether naturally occurring antibody levels against P. falciparum GLURP (PF3D7_1035300) and MSP3 (PF3D7_1035400) antigens are associated with decreased parasite load...
August 23, 2017: Parasites & Vectors
https://read.qxmd.com/read/28653041/-plasmodium-falciparum-genotypes-in-matched-peripheral-placental-and-umbilical-cord-blood-in-an-area-characterised-by-unstable-malaria-transmission-in-eastern-sudan
#25
JOURNAL ARTICLE
Omar E Fadlelseed, Maha E Osman, Nahla M Shamseldin, Amar B Elhussein, Ishag Adam
BACKGROUND: There are few published studies on Plasmodium falciparum genotypes in peripheral, placental and umbilical cord blood in areas characterised by unstable malaria transmission. METHOD: A cross-sectional study was conducted to investigate P. falciparum genotypes in matched peripheral, placental and umbilical cord blood in eastern Sudan. Thick blood smears and P. falciparum merozoite surface protein 1 (MSP1) and 2 (MSP2) genes as polymorphic markers in polymerase chain reactions were investigated in 3 kinds of samples of 153 pregnant women at delivery...
June 2017: Heliyon
https://read.qxmd.com/read/27938356/plasmodium-falciparum-genotype-and-gametocyte-prevalence-in-children-with-uncomplicated-malaria-in-coastal-ghana
#26
JOURNAL ARTICLE
Ruth Ayanful-Torgby, Akua Oppong, Joana Abankwa, Festus Acquah, Kimberly C Williamson, Linda Eva Amoah
BACKGROUND: Plasmodium falciparum gametocytes are vital to sustaining malaria transmission. Parasite densities, multiplicity of infection as well as asexual genotype are features that have been found to influence gametocyte production. Measurements of the prevalence of Plasmodium sp. gametocytes may serve as a tool to monitor the success of malaria eradication efforts. METHODS: Whole blood was collected from 112 children aged between 6 months and 13 years with uncomplicated P...
December 9, 2016: Malaria Journal
https://read.qxmd.com/read/27821442/critical-evaluation-of-molecular-monitoring-in-malaria-drug-efficacy-trials-and-pitfalls-of-length-polymorphic-markers
#27
JOURNAL ARTICLE
Camilla Messerli, Natalie E Hofmann, Hans-Peter Beck, Ingrid Felger
Estimation of drug efficacy in antimalarial drug trials requires parasite genotyping to distinguish new infections from treatment failures. When using length-polymorphic molecular markers, preferential amplification of short fragments can compromise detection of coinfections, potentially leading to misclassification of treatment outcome. We quantified minority clone detectability and competition among msp1, msp2, and glurp amplicons using mixtures of Plasmodium falciparum strains and investigated the impact of template competition on genotyping outcomes in 44 paired field samples...
January 2017: Antimicrobial Agents and Chemotherapy
https://read.qxmd.com/read/27259367/genetic-diversity-and-multiplicity-of-infection-of-plasmodium-falciparum-isolates-from-kolkata-west-bengal-india
#28
JOURNAL ARTICLE
Pabitra Saha, Swagata Ganguly, Ardhendu K Maji
The study of genetic diversity of Plasmodium falciparum is necessary to understand the distribution and dynamics of parasite populations. The genetic diversity of P. falciparum merozoite surface protein-1 and 2 has been extensively studied from different parts of world. However, limited data are available from India. This study was aimed to determine the genetic diversity and multiplicity of infection (MOI) of P. falciparum population in Kolkata, West Bengal, India. A total of 80day-zero blood samples from Kolkata were collected during a therapeutic efficacy study in 2008-2009...
September 2016: Infection, Genetics and Evolution
https://read.qxmd.com/read/27139455/plasmodium-falciparum-genetic-diversity-in-bangladesh-does-not-suggest-a-hypoendemic-population-structure
#29
JOURNAL ARTICLE
Mohammad Shafiul Alam, Rubayet Elahi, Abu Naser Mohon, Hasan Mohammad Al-Amin, Mohammad Golam Kibria, Wasif A Khan, Hamida Khanum, Rashidul Haque
Despite the recommendation for the use of merozoite surface protein 1 (msp1), merozoite surface protein 2 (msp2), and glutamate-rich protein (glurp) genes as markers in drug efficacy studies by World Health Organization and their limited use in Bangladesh, the circulating Plasmodium falciparum population genetic structure has not yet been assessed in Bangladesh. This study presents a comprehensive report on the circulating P. falciparum population structure based on msp1, msp2, and glurp polymorphic gene markers in Bangladesh...
June 1, 2016: American Journal of Tropical Medicine and Hygiene
https://read.qxmd.com/read/27110390/genetic-polymorphism-of-msp1-and-msp2-in-plasmodium-falciparum-isolates-from-c%C3%A3-te-d-ivoire-versus-gabon
#30
JOURNAL ARTICLE
William Yavo, Abibatou Konaté, Denise Patricia Mawili-Mboumba, Fulgence Kondo Kassi, Marie L Tshibola Mbuyi, Etienne Kpongbo Angora, Eby I Hervé Menan, Marielle K Bouyou-Akotet
Introduction. The characterization of genetic profile of Plasmodium isolates from different areas could help in better strategies for malaria elimination. This study aimed to compare P. falciparum diversity in two African countries. Methods. Isolates collected from 100 and 73 falciparum malaria infections in sites of Côte d'Ivoire (West Africa) and Gabon (Central Africa), respectively, were analyzed by a nested PCR amplification of msp1 and msp2 genes. Results. The K1 allelic family was widespread in Côte d'Ivoire (64...
2016: Journal of Parasitology Research
https://read.qxmd.com/read/26741287/genome-wide-association-study-of-antibody-responses-to-plasmodium-falciparum-candidate-vaccine-antigens
#31
JOURNAL ARTICLE
J Milet, A Sabbagh, F Migot-Nabias, A J F Luty, O Gaye, A Garcia, D Courtin
We conducted a genome-wide association study (GWAS) of antibody responses directed to three Plasmodium falciparum vaccine candidate antigens (MSP1, MSP2 and GLURP) previously associated with different patterns of protection against malaria infection in Senegalese children. A total of 174 950 single-nucleotide polymorphisms (SNPs) were tested for association with immunoglobulin G1 (IgG1) responses directed to MSP1 and to GLURP and with IgG3 responses to MSP2 FC27 and to MSP2 3D7. We first performed a single-trait analysis with each antibody response and then a multiple-trait analysis in which we analyzed simultaneously the three immune responses associated with the control of clinical malaria episodes...
March 2016: Genes and Immunity
https://read.qxmd.com/read/26626127/attenuation-of-plasmodium-falciparum-in-vitro-drug-resistance-phenotype-following-culture-adaptation-compared-to-fresh-clinical-isolates-in-cambodia
#32
COMPARATIVE STUDY
Suwanna Chaorattanakawee, Charlotte A Lanteri, Siratchana Sundrakes, Kritsanai Yingyuen, Panita Gosi, Nitima Chanarat, Saowaluk Wongarunkochakorn, Nillawan Buathong, Soklyda Chann, Worachet Kuntawunginn, Montri Arsanok, Jessica T Lin, Jonathan J Juliano, Stuart D Tyner, Mengchuor Char, Chanthap Lon, David L Saunders
BACKGROUND: There is currently no standardized approach for assessing in vitro anti-malarial drug susceptibility. Potential alterations in drug susceptibility results between fresh immediate ex vivo (IEV) and cryopreserved culture-adapted (CCA) Plasmodium falciparum isolates, as well as changes in parasite genotype during culture adaptation were investigated. METHODS: The 50 % inhibitory concentration (IC50) of 12 P. falciparum isolates from Cambodia against a panel of commonly used drugs were compared using both IEV and CCA...
2015: Malaria Journal
https://read.qxmd.com/read/26323839/allelic-diversity-of-msp1-gene-in-plasmodium-falciparum-from-rural-and-urban-areas-of-gabon
#33
JOURNAL ARTICLE
Denise Patricia Mawili-Mboumba, Noé Mbondoukwe, Elvire Adande, Marielle Karine Bouyou-Akotet
The present study determined and compared the genetic diversity of Plasmodium falciparum strains infecting children living in 2 areas from Gabon with different malaria endemicity. Blood samples were collected from febrile children from 2008 to 2009 in 2 health centres from rural (Oyem) and urban (Owendo) areas. Genetic diversity was determined in P. falciparum isolates by analyzing the merozoite surface protein-1 (msp1) gene polymorphism using nested-PCR. Overall, 168 children with mild falciparum malaria were included...
August 2015: Korean Journal of Parasitology
https://read.qxmd.com/read/26017553/intervals-to-plasmodium-falciparum-recurrence-after-anti-malarial-treatment-in-pregnancy-a-longitudinal-prospective-cohort
#34
JOURNAL ARTICLE
Natthapon Laochan, Sophie G Zaloumis, Mallika Imwong, Usa Lek-Uthai, Alan Brockman, Kanlaya Sriprawat, Jacher Wiladphaingern, Nicholas J White, François Nosten, Rose McGready
BACKGROUND: Plasmodium falciparum infections adversely affect pregnancy. Anti-malarial treatment failure is common. The objective of this study was to examine the duration of persistent parasite carriage following anti-malarial treatment in pregnancy. METHODS: The data presented here are a collation from previous studies carried out since 1994 in the Shoklo Malaria Research Unit (SMRU) on the Thailand-Myanmar border and performed using the same unique methodology detailed in the Materials and Methods section...
May 28, 2015: Malaria Journal
https://read.qxmd.com/read/24206588/evaluation-of-parasite-subpopulations-and-genetic-diversity-of-the-msp1-msp2-and-glurp-genes-during-and-following-artesunate-monotherapy-treatment-of-plasmodium-falciparum-malaria-in-western-cambodia
#35
JOURNAL ARTICLE
Panita Gosi, Charlotte A Lanteri, Stuart D Tyner, Youry Se, Chanthap Lon, Michele Spring, Mengchuor Char, Darapiseth Sea, Sabaithip Sriwichai, Sittidech Surasri, Saowaluk Wongarunkochakorn, Kingkan Pidtana, Douglas S Walsh, Mark M Fukuda, Jessica Manning, David L Saunders, Delia Bethell
BACKGROUND: Despite widespread coverage of the emergence of artemisinin resistance, relatively little is known about the parasite populations responsible. The use of PCR genotyping around the highly polymorphic Plasmodium falciparum msp1, msp2 and glurp genes has become well established both to describe variability in alleles within a population of parasites, as well as classify treatment outcome in cases of recurrent disease. The primary objective was to assess the emergence of minority parasite clones during seven days of artesunate (AS) treatment in a location with established artemisinin resistance...
November 9, 2013: Malaria Journal
https://read.qxmd.com/read/24117665/fc-gamma-receptor-iia-h131r-polymorphism-and-malaria-susceptibility-in-sympatric-ethnic-groups-fulani-and-dogon-of-mali
#36
JOURNAL ARTICLE
B Maiga, A Dolo, O Touré, V Dara, A Tapily, S Campino, N Sepulveda, P Corran, K Rockett, T G Clark, M Troye Blomberg, O K Doumbo
It has been previously shown that there are some interethnic differences in susceptibility to malaria between two sympatric ethnic groups of Mali, the Fulani and the Dogon. The lower susceptibility to Plasmodium falciparum malaria seen in the Fulani has not been fully explained by genetic polymorphisms previously known to be associated with malaria resistance, including haemoglobin S (HbS), haemoglobin C (HbC), alpha-thalassaemia and glucose-6-phosphate dehydrogenase (G6PD) deficiency. Given the observed differences in the distribution of FcγRIIa allotypes among different ethnic groups and with malaria susceptibility that have been reported, we analysed the rs1801274-R131H polymorphism in the FcγRIIa gene in a study of Dogon and Fulani in Mali (n = 939)...
January 2014: Scandinavian Journal of Immunology
https://read.qxmd.com/read/24098393/human-candidate-polymorphisms-in-sympatric-ethnic-groups-differing-in-malaria-susceptibility-in-mali
#37
JOURNAL ARTICLE
Bakary Maiga, Amagana Dolo, Ousmane Touré, Victor Dara, Amadou Tapily, Susana Campino, Nuno Sepulveda, Paul Risley, Nilupa Silva, Nipula Silva, Patrick Corran, Kirk A Rockett, Dominic Kwiatkowski, Taane G Clark, Marita Troye-Blomberg, Ogobara K Doumbo
Malaria still remains a major public health problem in Mali, although disease susceptibility varies between ethnic groups, particularly between the Fulani and Dogon. These two sympatric groups share similar socio-cultural factors and malaria transmission rates, but Fulani individuals tend to show significantly higher spleen enlargement scores, lower parasite prevalence, and seem less affected by the disease than their Dogon neighbours. We have used genetic polymorphisms from malaria-associated genes to investigate associations with various malaria metrics between the Fulanai and Dogon groups...
2013: PloS One
https://read.qxmd.com/read/23641369/genetic-diversity-of-plasmodium-falciparum-field-isolates-in-central-sudan-inferred-by-pcr-genotyping-of-merozoite-surface-protein-1-and-2
#38
JOURNAL ARTICLE
Muzamil M Abdel Hamid, Sara B Mohammed, Ibrahim M El Hassan
BACKGROUND: Characterization of Plasmodium falciparum diversity is commonly achieved by amplification of the polymorphic regions of the merozoite surface proteins 1 (MSP1) and 2 (MSP2) genes. AIMS: The present study aimed to determine the allelic variants distribution of MSP1 and MSP2 and multiplicity of infection in P. falciparum field isolates from Kosti, central Sudan, an area characterized by seasonal malaria transmission. MATERIALS AND METHODS: Total 121 samples (N = 121) were collected during a cross-sectional survey between March and April 2003...
February 2013: North American Journal of Medical Sciences
https://read.qxmd.com/read/23408340/genetic-diversity-of-plasmodium-falciparum-infections-in-mild-and-severe-malaria-of-children-from-kampala-uganda
#39
JOURNAL ARTICLE
Mpungu Steven Kiwuwa, Ulf Ribacke, Kirsten Moll, Justus Byarugaba, Klara Lundblom, Anna Färnert, Kironde Fred, Mats Wahlgren
Diversity in parasite virulence is one of the factors that contribute to the clinical outcome of malaria infections. The association between the severity of Plasmodium falciparum malaria and the number of distinct parasite populations infecting the host (multiplicity of infection) or polymorphism within any of the specific antigen genes was investigated. The study included 164 children presenting with mild and severe malaria from central Uganda where malaria is meso-endemic. The polymorphic regions of the circumsporozoite protein (csp), merozoite surface proteins 1 and 2 (msp1 and msp2), and glutamate-rich protein (glurp) were genotyped by polymerase chain reaction methods and fragment analysis by gel electrophoresis...
April 2013: Parasitology Research
https://read.qxmd.com/read/23000544/plasmodium-falciparum-populations-from-northeastern-myanmar-display-high-levels-of-genetic-diversity-at-multiple-antigenic-loci
#40
JOURNAL ARTICLE
Lili Yuan, Hui Zhao, Lanou Wu, Xiaomei Li, Daniel Parker, Shuhui Xu, Yousheng Zhao, Guohua Feng, Ying Wang, Guiyun Yan, Qi Fan, Zhaoqing Yang, Liwang Cui
Levels of genetic diversity of the malaria parasites and multiclonal infections are correlated with transmission intensity. In order to monitor the effect of strengthened malaria control efforts in recent years at the China-Myanmar border area, we followed the temporal dynamics of genetic diversity of three polymorphic antigenic markers msp1, msp2, and glurp in the Plasmodium falciparum populations. Despite reduced malaria prevalence in the region, parasite populations exhibited high levels of genetic diversity...
January 2013: Acta Tropica
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