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falciparum polymorphism msp1 msp2

L E Amoah, S V Nuvor, E K Obboh, F K Acquah, K Asare, S K Singh, J N Boampong, M Theisen, K C Williamson
BACKGROUND: Plasmodium falciparum genetic diversity and multiplicity of infection (MOI) are parasite features that have been suggested to influence the acquisition of protective immunity against malaria. This study sought to assess the relationship between MOI and parasite density (PD) in malaria patients living in the Central Region of Ghana and to determine whether naturally occurring antibody levels against P. falciparum GLURP (PF3D7_1035300) and MSP3 (PF3D7_1035400) antigens are associated with decreased parasite load...
August 23, 2017: Parasites & Vectors
Omar E Fadlelseed, Maha E Osman, Nahla M Shamseldin, Amar B Elhussein, Ishag Adam
BACKGROUND: There are few published studies on Plasmodium falciparum genotypes in peripheral, placental and umbilical cord blood in areas characterised by unstable malaria transmission. METHOD: A cross-sectional study was conducted to investigate P. falciparum genotypes in matched peripheral, placental and umbilical cord blood in eastern Sudan. Thick blood smears and P. falciparum merozoite surface protein 1 (MSP1) and 2 (MSP2) genes as polymorphic markers in polymerase chain reactions were investigated in 3 kinds of samples of 153 pregnant women at delivery...
June 2017: Heliyon
Ruth Ayanful-Torgby, Akua Oppong, Joana Abankwa, Festus Acquah, Kimberly C Williamson, Linda Eva Amoah
BACKGROUND: Plasmodium falciparum gametocytes are vital to sustaining malaria transmission. Parasite densities, multiplicity of infection as well as asexual genotype are features that have been found to influence gametocyte production. Measurements of the prevalence of Plasmodium sp. gametocytes may serve as a tool to monitor the success of malaria eradication efforts. METHODS: Whole blood was collected from 112 children aged between 6 months and 13 years with uncomplicated P...
December 9, 2016: Malaria Journal
Camilla Messerli, Natalie E Hofmann, Hans-Peter Beck, Ingrid Felger
Estimation of drug efficacy in antimalarial drug trials requires parasite genotyping to distinguish new infections from treatment failures. When using length-polymorphic molecular markers, preferential amplification of short fragments can compromise detection of coinfections, potentially leading to misclassification of treatment outcome. We quantified minority clone detectability and competition among msp1, msp2, and glurp amplicons using mixtures of Plasmodium falciparum strains and investigated the impact of template competition on genotyping outcomes in 44 paired field samples...
January 2017: Antimicrobial Agents and Chemotherapy
Pabitra Saha, Swagata Ganguly, Ardhendu K Maji
The study of genetic diversity of Plasmodium falciparum is necessary to understand the distribution and dynamics of parasite populations. The genetic diversity of P. falciparum merozoite surface protein-1 and 2 has been extensively studied from different parts of world. However, limited data are available from India. This study was aimed to determine the genetic diversity and multiplicity of infection (MOI) of P. falciparum population in Kolkata, West Bengal, India. A total of 80day-zero blood samples from Kolkata were collected during a therapeutic efficacy study in 2008-2009...
September 2016: Infection, Genetics and Evolution
Mohammad Shafiul Alam, Rubayet Elahi, Abu Naser Mohon, Hasan Mohammad Al-Amin, Mohammad Golam Kibria, Wasif A Khan, Hamida Khanum, Rashidul Haque
Despite the recommendation for the use of merozoite surface protein 1 (msp1), merozoite surface protein 2 (msp2), and glutamate-rich protein (glurp) genes as markers in drug efficacy studies by World Health Organization and their limited use in Bangladesh, the circulating Plasmodium falciparum population genetic structure has not yet been assessed in Bangladesh. This study presents a comprehensive report on the circulating P. falciparum population structure based on msp1, msp2, and glurp polymorphic gene markers in Bangladesh...
June 1, 2016: American Journal of Tropical Medicine and Hygiene
William Yavo, Abibatou Konaté, Denise Patricia Mawili-Mboumba, Fulgence Kondo Kassi, Marie L Tshibola Mbuyi, Etienne Kpongbo Angora, Eby I Hervé Menan, Marielle K Bouyou-Akotet
Introduction. The characterization of genetic profile of Plasmodium isolates from different areas could help in better strategies for malaria elimination. This study aimed to compare P. falciparum diversity in two African countries. Methods. Isolates collected from 100 and 73 falciparum malaria infections in sites of Côte d'Ivoire (West Africa) and Gabon (Central Africa), respectively, were analyzed by a nested PCR amplification of msp1 and msp2 genes. Results. The K1 allelic family was widespread in Côte d'Ivoire (64...
2016: Journal of Parasitology Research
J Milet, A Sabbagh, F Migot-Nabias, A J F Luty, O Gaye, A Garcia, D Courtin
We conducted a genome-wide association study (GWAS) of antibody responses directed to three Plasmodium falciparum vaccine candidate antigens (MSP1, MSP2 and GLURP) previously associated with different patterns of protection against malaria infection in Senegalese children. A total of 174 950 single-nucleotide polymorphisms (SNPs) were tested for association with immunoglobulin G1 (IgG1) responses directed to MSP1 and to GLURP and with IgG3 responses to MSP2 FC27 and to MSP2 3D7. We first performed a single-trait analysis with each antibody response and then a multiple-trait analysis in which we analyzed simultaneously the three immune responses associated with the control of clinical malaria episodes...
March 2016: Genes and Immunity
Suwanna Chaorattanakawee, Charlotte A Lanteri, Siratchana Sundrakes, Kritsanai Yingyuen, Panita Gosi, Nitima Chanarat, Saowaluk Wongarunkochakorn, Nillawan Buathong, Soklyda Chann, Worachet Kuntawunginn, Montri Arsanok, Jessica T Lin, Jonathan J Juliano, Stuart D Tyner, Mengchuor Char, Chanthap Lon, David L Saunders
BACKGROUND: There is currently no standardized approach for assessing in vitro anti-malarial drug susceptibility. Potential alterations in drug susceptibility results between fresh immediate ex vivo (IEV) and cryopreserved culture-adapted (CCA) Plasmodium falciparum isolates, as well as changes in parasite genotype during culture adaptation were investigated. METHODS: The 50 % inhibitory concentration (IC50) of 12 P. falciparum isolates from Cambodia against a panel of commonly used drugs were compared using both IEV and CCA...
2015: Malaria Journal
Denise Patricia Mawili-Mboumba, Noé Mbondoukwe, Elvire Adande, Marielle Karine Bouyou-Akotet
The present study determined and compared the genetic diversity of Plasmodium falciparum strains infecting children living in 2 areas from Gabon with different malaria endemicity. Blood samples were collected from febrile children from 2008 to 2009 in 2 health centres from rural (Oyem) and urban (Owendo) areas. Genetic diversity was determined in P. falciparum isolates by analyzing the merozoite surface protein-1 (msp1) gene polymorphism using nested-PCR. Overall, 168 children with mild falciparum malaria were included...
August 2015: Korean Journal of Parasitology
Natthapon Laochan, Sophie G Zaloumis, Mallika Imwong, Usa Lek-Uthai, Alan Brockman, Kanlaya Sriprawat, Jacher Wiladphaingern, Nicholas J White, François Nosten, Rose McGready
BACKGROUND: Plasmodium falciparum infections adversely affect pregnancy. Anti-malarial treatment failure is common. The objective of this study was to examine the duration of persistent parasite carriage following anti-malarial treatment in pregnancy. METHODS: The data presented here are a collation from previous studies carried out since 1994 in the Shoklo Malaria Research Unit (SMRU) on the Thailand-Myanmar border and performed using the same unique methodology detailed in the Materials and Methods section...
May 28, 2015: Malaria Journal
Panita Gosi, Charlotte A Lanteri, Stuart D Tyner, Youry Se, Chanthap Lon, Michele Spring, Mengchuor Char, Darapiseth Sea, Sabaithip Sriwichai, Sittidech Surasri, Saowaluk Wongarunkochakorn, Kingkan Pidtana, Douglas S Walsh, Mark M Fukuda, Jessica Manning, David L Saunders, Delia Bethell
BACKGROUND: Despite widespread coverage of the emergence of artemisinin resistance, relatively little is known about the parasite populations responsible. The use of PCR genotyping around the highly polymorphic Plasmodium falciparum msp1, msp2 and glurp genes has become well established both to describe variability in alleles within a population of parasites, as well as classify treatment outcome in cases of recurrent disease. The primary objective was to assess the emergence of minority parasite clones during seven days of artesunate (AS) treatment in a location with established artemisinin resistance...
November 9, 2013: Malaria Journal
B Maiga, A Dolo, O Touré, V Dara, A Tapily, S Campino, N Sepulveda, P Corran, K Rockett, T G Clark, M Troye Blomberg, O K Doumbo
It has been previously shown that there are some interethnic differences in susceptibility to malaria between two sympatric ethnic groups of Mali, the Fulani and the Dogon. The lower susceptibility to Plasmodium falciparum malaria seen in the Fulani has not been fully explained by genetic polymorphisms previously known to be associated with malaria resistance, including haemoglobin S (HbS), haemoglobin C (HbC), alpha-thalassaemia and glucose-6-phosphate dehydrogenase (G6PD) deficiency. Given the observed differences in the distribution of FcγRIIa allotypes among different ethnic groups and with malaria susceptibility that have been reported, we analysed the rs1801274-R131H polymorphism in the FcγRIIa gene in a study of Dogon and Fulani in Mali (n = 939)...
January 2014: Scandinavian Journal of Immunology
Bakary Maiga, Amagana Dolo, Ousmane Touré, Victor Dara, Amadou Tapily, Susana Campino, Nuno Sepulveda, Paul Risley, Nilupa Silva, Nipula Silva, Patrick Corran, Kirk A Rockett, Dominic Kwiatkowski, Taane G Clark, Marita Troye-Blomberg, Ogobara K Doumbo
Malaria still remains a major public health problem in Mali, although disease susceptibility varies between ethnic groups, particularly between the Fulani and Dogon. These two sympatric groups share similar socio-cultural factors and malaria transmission rates, but Fulani individuals tend to show significantly higher spleen enlargement scores, lower parasite prevalence, and seem less affected by the disease than their Dogon neighbours. We have used genetic polymorphisms from malaria-associated genes to investigate associations with various malaria metrics between the Fulanai and Dogon groups...
2013: PloS One
Muzamil M Abdel Hamid, Sara B Mohammed, Ibrahim M El Hassan
BACKGROUND: Characterization of Plasmodium falciparum diversity is commonly achieved by amplification of the polymorphic regions of the merozoite surface proteins 1 (MSP1) and 2 (MSP2) genes. AIMS: The present study aimed to determine the allelic variants distribution of MSP1 and MSP2 and multiplicity of infection in P. falciparum field isolates from Kosti, central Sudan, an area characterized by seasonal malaria transmission. MATERIALS AND METHODS: Total 121 samples (N = 121) were collected during a cross-sectional survey between March and April 2003...
February 2013: North American Journal of Medical Sciences
Mpungu Steven Kiwuwa, Ulf Ribacke, Kirsten Moll, Justus Byarugaba, Klara Lundblom, Anna Färnert, Kironde Fred, Mats Wahlgren
Diversity in parasite virulence is one of the factors that contribute to the clinical outcome of malaria infections. The association between the severity of Plasmodium falciparum malaria and the number of distinct parasite populations infecting the host (multiplicity of infection) or polymorphism within any of the specific antigen genes was investigated. The study included 164 children presenting with mild and severe malaria from central Uganda where malaria is meso-endemic. The polymorphic regions of the circumsporozoite protein (csp), merozoite surface proteins 1 and 2 (msp1 and msp2), and glutamate-rich protein (glurp) were genotyped by polymerase chain reaction methods and fragment analysis by gel electrophoresis...
April 2013: Parasitology Research
Lili Yuan, Hui Zhao, Lanou Wu, Xiaomei Li, Daniel Parker, Shuhui Xu, Yousheng Zhao, Guohua Feng, Ying Wang, Guiyun Yan, Qi Fan, Zhaoqing Yang, Liwang Cui
Levels of genetic diversity of the malaria parasites and multiclonal infections are correlated with transmission intensity. In order to monitor the effect of strengthened malaria control efforts in recent years at the China-Myanmar border area, we followed the temporal dynamics of genetic diversity of three polymorphic antigenic markers msp1, msp2, and glurp in the Plasmodium falciparum populations. Despite reduced malaria prevalence in the region, parasite populations exhibited high levels of genetic diversity...
January 2013: Acta Tropica
Rajika L Dewasurendra, Prapat Suriyaphol, Sumadhya D Fernando, Richard Carter, Kirk Rockett, Patrick Corran, Dominic Kwiatkowski, Nadira D Karunaweera
BACKGROUND: The incidence of malaria in Sri Lanka has significantly declined in recent years. Similar trends were seen in Kataragama, a known malaria endemic location within the southern province of the country, over the past five years. This is a descriptive study of anti-malarial antibody levels and selected host genetic mutations in residents of Kataragama, under low malaria transmission conditions. METHODS: Sera were collected from 1,011 individuals residing in Kataragama and anti-malarial antibodies and total IgE levels were measured by a standardized ELISA technique...
August 20, 2012: Malaria Journal
Sandra Milena Barrera, Manuel Alberto Pérez, Angélica Knudson, Rubén Santiago Nicholls, Angela Patricia Guerra
INTRODUCTION: The genetic diversity of Plasmodium falciparum has been one of the major obstacles for the success of anti-malaria drug therapy. It provides the parasite an ability to evade the host's immune response by generating changes in its antigenic composition and resistance to antimalarial drugs. OBJECTIVE: The genetic diversity of P.falciparum was characterized in 4 Colombian localities through the analysis of polymorphic genes. MATERIALS AND METHODS: Eighty-one samples were obtained from patients with uncomplicated P...
October 2010: Biomédica: Revista del Instituto Nacional de Salud
Felista Mwingira, Gamba Nkwengulila, Sonja Schoepflin, Deborah Sumari, Hans-Peter Beck, Georges Snounou, Ingrid Felger, Piero Olliaro, Kefas Mugittu
BACKGROUND: The efficacy of anti-malarial drugs is assessed over a period of 28-63 days (depending on the drugs' residence time) following initiation of treatment in order to capture late failures. However, prolonged follow-up increases the likelihood of new infections depending on transmission intensity. Therefore, molecular genotyping of highly polymorphic regions of Plasmodium falciparum msp1, msp2 and glurp loci is usually carried out to distinguish recrudescence (true failures) from new infections...
April 6, 2011: Malaria Journal
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