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Retinal stem cells

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https://www.readbyqxmd.com/read/29132470/-effects-of-bone-marrow-mesenchymal-stem-cell-transplantation-on-retinal-neovascularization-in-neonatal-rats-with-oxygen-induced-retinopathy
#1
Qing-Jie Mu, Yue-Hua Zhao, Dan-Dan Cheng, Hai-Yu Wang, Lan-Fen Chen, Yan-Song Zhao, Xiao-Li Wang
OBJECTIVE: To explore the effects of rat bone mesenchymal stem cell (BMSC) transplantation on retinal neovascularization, and to observe the changes of hypoxia-inducible factor-1 alpha (HIF-1α) and vascular endothelial growth factors (VEGF) in rats with oxygen-induced retinopathy (OIR). METHODS: Seventy-two seven-day-old Sprague-Dawley rats were randomly divided into three groups: normal control (CON), model (OIR) and BMSC transplantation. In the BMSC transplantation group, BMSCs were transplanted 5 days after oxygen conditioning...
November 2017: Zhongguo Dang Dai Er Ke za Zhi, Chinese Journal of Contemporary Pediatrics
https://www.readbyqxmd.com/read/29130158/crispr-in-the-retina-evaluation-of-future-potential
#2
Galaxy Y Cho, Sally Justus, Jesse D Sengillo, Stephen H Tsang
Clustered regularly interspaced short palindromic repeats (CRISPR) has been gaining widespread attention for its ability for targeted genome surgery. In treating inherited retinal degenerations, gene therapies have had varied results; the ones effective in restoring eye sight are limited by transiency in its effect. Genome surgery, however, is a solution that could potentially provide the eye with permanent healthy cells. As retinal degenerations are irreversible and the retina has little regenerative potential, permanent healthy cells are vital for vision...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29128844/a-biodegradable-scaffold-enhances-differentiation-of-embryonic-stem-cells-into-a-thick-sheet-of-retinal-cells
#3
Deepti Singh, Shao-Bin Wang, Tina Xia, Laurel Tainsh, Maryam Ghiassi-Nejad, Tao Xu, Shaomin Peng, Ron A Adelman, Lawrence J Rizzolo
Retinal degeneration is a leading cause of blindness in developed countries. Stem cells can be differentiated into retinal organoids to study mechanisms of retinal degeneration, develop therapeutic agents, and potentially serve as replacement tissues. The spherical nature of these retinoids limits their utility, because the investigator lacks ready access to both sides of the neo-tissue. For tissue-replacement, spherical retinoids are unable to interact simultaneously with the host retinal pigment epithelium and remaining neurosensory retina...
October 31, 2017: Biomaterials
https://www.readbyqxmd.com/read/29116192/human-stem-cell-derived-retinal-epithelial-cells-activate-complement-via-collectin-11-in-response-to-stress
#4
Giorgia Fanelli, Anai Gonzalez-Cordero, Peter J Gardner, Qi Peng, Milan Fernando, Magdalena Kloc, Conrad A Farrar, Arifa Naeem, Peter Garred, Robin R Ali, Steven H Sacks
Age-related macular degeneration (AMD) is a major cause of blindness and is associated with complement dysregulation. The disease is a potential target for stem cell therapy but success is likely to be limited by the inflammatory response. We investigated the innate immune properties of human induced-pluripotent stem cell (iPSC)-derived RPE cells, particularly with regard to the complement pathway. We focused on collectin-11 (CL-11), a pattern recognition molecule that can trigger complement activation in renal epithelial tissue...
November 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29113466/perspectives-of-stem-cell-based-therapy-for-age-related-retinal-degenerative-diseases
#5
Vladimir Holan, Barbora Hermankova, Jan Kossl
Retinal degenerative diseases, which include age-related macular degeneration, retinitis pigmentosa, diabetic retinopathy, and glaucoma, mostly affect the elderly population and are the most common cause of decreased quality of vision or even blindness. So far, there is no satisfactory treatment protocol to prevent, stop, or cure these disorders. A great hope and promise for patients suffering from retinal diseases is represented by stem cell-based therapy that could replace diseased or missing retinal cells and support regeneration...
September 2017: Cell Transplantation
https://www.readbyqxmd.com/read/29111262/overcoming-monocarboxylate-transporter-8-mct8-deficiency-to-promote-human-oligodendrocyte-differentiation-and-myelination
#6
Jae Young Lee, Min Joung Kim, Devy Deliyanti, Michael F Azari, Fernando Rossello, Adam Costin, Georg Ramm, Edouard G Stanley, Andrew G Elefanty, Jennifer L Wilkinson-Berka, Steven Petratos
Cell membrane thyroid hormone (TH) transport can be facilitated by the monocarboxylate transporter 8 (MCT8), encoded by the solute carrier family 16 member 2 (SLC16A2) gene. Human mutations of the gene, SLC16A2, result in the X-linked-inherited psychomotor retardation and hypomyelination disorder, Allan-Herndon-Dudley syndrome (AHDS). We posited that abrogating MCT8-dependent TH transport limits oligodendrogenesis and myelination. We show that human oligodendrocytes (OL), derived from the NKX2.1-GFP human embryonic stem cell (hESC) reporter line, express MCT8...
October 19, 2017: EBioMedicine
https://www.readbyqxmd.com/read/29103970/detection-of-retinal-pigment-epithelium-specific-antibody-in-ipsc-derived-retinal-pigment-epithelium-transplantation-models
#7
Sunao Sugita, Kenichi Makabe, Shota Fujii, Yuko Iwasaki, Hiroyuki Kamao, Takashi Shiina, Kazumasa Ogasawara, Masayo Takahashi
Antibody-mediated rejection is characterized by donor-specific antibody produced by B cells. However, to our knowledge, B cell invasion and antibody in the inflamed retina after transplantation of retinal pigment epithelial (RPE) cells has not been reported. To determine if RPE transplantation could be performed using allografts, we established in vivo immune rejection models with induced pluripotent stem cell (iPSC)-RPE allografts and determined whether RPE-specific antibody could be detected in these models...
November 14, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/29093829/development-of-a-new-tissue-injector-for-subretinal-transplantation-of-human-embryonic-stem-cell-derived-retinal-pigmented-epithelium
#8
Rodrigo A Brant Fernandes, Francisco R Stefanini, Paulo Falabella, Michael J Koss, Trent Wells, Bruno Diniz, Ramiro Ribeiro, Paulo Schor, Mauricio Maia, Fernando M Penha, David R Hinton, Yu-Chong Tai, Mark Humayun
Background: Subretinal cell transplantation is a challenging surgical maneuver. This paper describes the preliminary findings of a new tissue injector for subretinal implantation of an ultrathin non-absorbable substrate seeded with human embryonic stem cell-derived retinal pigment epithelium (hESC-RPE). Methods: Ultrathin Parylene-C substrates measuring 3.5 mm × 6.0 mm seeded with hESC-RPE (implant referred to as CPCB-RPE1) were implanted into the subretinal space of 12 Yucatan minipigs...
2017: International Journal of Retina and Vitreous
https://www.readbyqxmd.com/read/29062767/differentiation-of-human-olfactory-mucosa-mesenchymal-stem-cells-into-photoreceptor-cells-in-vitro
#9
Wen Lu, Da Duan, Zacharia Ackbarkhan, Ming Lu, Min-Li Huang
AIM: To investigate whether the human olfactory mucosa mesenchymal stem cells (OM-MSCs) can differentiate into photoreceptor cells in vitro. METHODS: Through the olfactory mucosa adherent method, olfactory mucosa was isolated, cultured and identified in vitro among mesenchymal stem cells. The cell surface markers were analyzed by flow cytometry, induced to differentiate into retinal photoreceptor cells in vitro, and the expression of rhodopsin was observed and identified by Immunofluorescence and Western blot methods...
2017: International Journal of Ophthalmology
https://www.readbyqxmd.com/read/29061667/novel-yap1-activator-identified-by-transcription-based-functional-screen-limits-multiple-myeloma-growth
#10
Junichi Maruyama, Kazutoshi Inami, Fumiyoshi Michishita, Xinliang Jiang, Hiroaki Iwasa, Kentaro Nakagawa, Mari Ishigami-Yuasa, Hiroyuki Kagechika, Norio Miyamura, Jun Hirayama, Hiroshi Nishina, Daichi Nogawa, Kouhei Yamamoto, Yutaka Hata
Yes-associated protein 1 (YAP1) interacts with numerous transcription factors including TEA-domain family proteins (TEAD) and p73. YAP1 is negatively regulated by the tumor suppressor Hippo pathway. In human cancers, the deregulation of the Hippo pathway and YAP1 gene amplification lead to the activation of YAP1, which induces epithelial-mesenchymal transition (EMT) and drug resistance. YAP1 inhibitors are expected to be useful in cancer therapy. On the other hand, in certain cancers, YAP1 up-regulates p73-dependent gene transcription and behaves as a tumor suppressor...
October 23, 2017: Molecular Cancer Research: MCR
https://www.readbyqxmd.com/read/29060856/a-targeted-drug-delivery-platform-for-assisting-retinal-surgeons-for-treating-age-related-macular-degeneration-amd
#11
M A Nasseri, M Maier, C P Lohmann
In this paper we present our latest robotic setup, which has been modified for sub-retinal interventions. The setup consists of: 1) sub-retinal micro cannula with automatic pump; 2) Micromanipulator; 3) patient fixation mechanism and 4) clinically compatible workstation. The primary objective of this work is to allow ophthalmologists to improve administration of substances such as drugs, stem-cells and gene cargos to their desired targets in the sub-retinal microstructures. Such a delivery method will enable effective treatment of Age-related Macular Degeneration (AMD)...
July 2017: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/29057815/further-insights-into-the-ciliary-gene-and-protein-kiz-and-its-murine-ortholog-plk1s1-mutated-in-rod-cone-dystrophy
#12
Said El Shamieh, Cécile Méjécase, Matteo Bertelli, Angélique Terray, Christelle Michiels, Christel Condroyer, Stéphane Fouquet, Maxime Sadoun, Emmanuelle Clérin, Binqian Liu, Thierry Léveillard, Olivier Goureau, José-Alain Sahel, Isabelle Audo, Christina Zeitz
We identified herein additional patients with rod-cone dystrophy (RCD) displaying mutations in KIZ, encoding the ciliary centrosomal protein kizuna and performed functional characterization of the respective protein in human fibroblasts and of its mouse ortholog PLK1S1 in the retina. Mutation screening was done by targeted next generation sequencing and subsequent Sanger sequencing validation. KIZ mRNA levels were assessed on blood and serum-deprived human fibroblasts from a control individual and a patient, compound heterozygous for the c...
October 18, 2017: Genes
https://www.readbyqxmd.com/read/29057252/glis1-3-emerging-roles-in-reprogramming-stem-and-progenitor-cell-differentiation-and-maintenance
#13
REVIEW
David W Scoville, Hong Soon Kang, Anton M Jetten
Recent studies have provided evidence for a regulatory role of GLI-similar (GLIS) transcription factors in reprogramming, maintenance and differentiation of several stem and progenitor cell populations. GLIS1, in conjunction with several other reprogramming factors, was shown to markedly increase the efficiency of generating induced pluripotent stem cells (iPSC) from somatic cells. GLIS2 has been reported to contribute to the maintenance of the pluripotent state in hPSCs. In addition, GLIS2 has a function in regulating self-renewal of hematopoietic progenitors and megakaryocytic differentiation...
2017: Stem Cell Investigation
https://www.readbyqxmd.com/read/29050915/decellularized-matrix-of-adipose-derived-mesenchymal-stromal-cells-enhanced-retinal-progenitor-cell-proliferation-via-the-akt-erk-pathway-and-neuronal-differentiation
#14
Jing Ji, Dandan Zhang, Wei Wei, Bingqiao Shen, Yi Zhang, Yuyao Wang, Zhimin Tang, Ni Ni, Hao Sun, Jiaqiang Liu, Xianqun Fan, Ping Gu
BACKGROUND AIMS: Retinal progenitor cells (RPCs) are a promising cell therapy treatment for retinal degenerative diseases. However, problems with limited proliferation ability and differentiation preference toward glia rather than neurons restrict the clinical application of these RPCs. The extracellular matrix (ECM) has been recognized to provide an appropriate microenvironment to support stem cell adhesion and direct cell behaviors, such as self-renewal and differentiation. METHODS: In this study, decellularized matrix of adipose-derived mesenchymal stromal cells (DMA) was manufactured using a chemical agent method (0...
October 16, 2017: Cytotherapy
https://www.readbyqxmd.com/read/29050186/-influent-factors-for-treating-procedure-of-cytomegalovirus-retinitis-after-allogeneic-bone-marrow-hematopoietic-stem-cell-transplantation
#15
H Miao, J Hou
Objective: To explore clinical and laboratory factors that influencing treating procedure of cytomegalovirus retinitis (CMVR) after allogeneic bone marrow hematopoietic stem cell transplantation (HSCT). Methods: This is a retrospective case series study. A total of 9 CMVR patients (15 eyes) between January 2016 and March 2017 were included in this study. All patients received intravenous or oral ganciclovir, together with intravitreous injection of ganciclovir alone or combined with foscanet sodium. One day before the first injection, aqueous humor samples from the affected eyes were collected, and CMV-DNA and interleukin-8 (IL-8) level were examined...
October 11, 2017: [Zhonghua Yan Ke za Zhi] Chinese Journal of Ophthalmology
https://www.readbyqxmd.com/read/29049869/collagen-vitrigels-with-low-fibril-density-enhance-human-embryonic-stem-cell-derived-retinal-pigment-epithelial-cell-maturation
#16
Xiaokun Wang, Julien Maruotti, Shoumyo Majumdar, Jose Roman, Hai-Quan Mao, Donald Zack, Jennifer Elisseeff
Structural and biochemical cues of extracellular matrix can substantially influence the differentiation and maturation of cultured retinal pigment epithelial cells (RPE). In this study, thin collagen vitrigels were engineered to create collagen nanofibrillar structures of different fibril densities in an effort to evaluate the maturation of human embryonic stem cell-derived retinal pigment epithelial cells (hESC-RPE). The ultrastructure of the different collagen vitrigels was characterized by transmission electron microscopy, and the mechanical properties was evaluated by tensile testing...
October 19, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29049728/surgically-induced-focal-retinal-detachment-does-not-cause-detectable-sd-oct-retinal-changes-in-normal-human-retina
#17
MULTICENTER STUDY
Kaitlin Kogachi, Jeremy D Wolfe, Amir H Kashani
Purpose: Induction of focal retinal detachment (RD) for subretinal delivery of stem cells and gene therapy is increasingly common. In order to determine if this procedure has an adverse impact on the retina, we use spectral-domain optical coherence tomography (SD-OCT) to evaluate the pre- and postoperative retinal anatomy of the incidentally detached normal retina surrounding large submacular hemorrhages (SMH) during surgical displacement procedures. Methods: Retrospective, observational study of human subjects with monocular SMH evaluated before and after surgical displacement using clinical exam, fundus photography, and SD-OCT...
October 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29047024/generation-of-photoreceptor-precursors-from-mouse-embryonic-stem-cells
#18
Amy Q Lu, Colin J Barnstable
Embryonic stem cell (ESC) differentiation can be used to model development and to produce transplantable cells of the desired phenotype. ESCs can reproducibly generate retinal cells but the derivation of photoreceptor precursors is variable and depends on an array of exogenous factors and intrinsic cell-cell interactions. In this work, we have defined the use of exogenous signaling factors, dissociation, and adherent versus 3-dimensional (3D) conditions on the derivation of retinal cells from pluripotent mouse ESCs...
October 18, 2017: Stem Cell Reviews
https://www.readbyqxmd.com/read/29044808/neuroprotective-effects-of-human-mesenchymal-stem-cells-and-platelet-derived-growth-factor-on-human-retinal-ganglion-cells
#19
Andrew Osborne, Julie Sanderson, Keith R Martin
Optic neuropathies such as glaucoma occur when retinal ganglion cells (RGCs) in the eye are injured. Strong evidence suggests mesenchymal stem cells (MSCs) could be a potential therapy to protect RGCs, however little is known regarding their effect on the human retina. We, therefore, investigated if human MSCs (hMSCs), or platelet-derived growth factor (PDGF) as produced by hMSC, could delay RGC death in a human retinal explant model of optic nerve injury. Our results showed hMSCs and the secreted growth factor PDGF-AB could substantially reduce human RGC loss and apoptosis following axotomy...
October 17, 2017: Stem Cells
https://www.readbyqxmd.com/read/29042966/effects-of-rpe-conditioned-medium-on-the-differentiation-of-hadscs-into-rpe-cells-and-their-proliferation-and-migration
#20
Yi Zhang, Dandan Zhang, Wei Wei, Bingqiao Shen, Yuyao Wang, Yingjie Zhang, Yidan Zhang, Jing Ji, Hao Sun, Min Luo, Ping Gu
Age-related macular degeneration (AMD) is associated with the dysfunction and death of the retinal pigment epithelium (RPE). Recently, there has been increasing interest in stem cell-derived RPE cells for cell replacement therapies, such as those for AMD. The present study investigated whether RPE-conditioned medium (RPECM) could promote the differentiation of human adipose tissue-derived mesenchymal stromal cells (hADSCs) into RPE cells, and enhance the proliferation and migration of these cells. Reverse-transcription quantitative polymerase chain reaction analysis demonstrated that RPECM induced hADSCs to differentiate into cells expressing RPE markers, including retinoid isomerohydrolase (RPE65), cytokeratin (CK8) and Bestrophin, which were identified to be significantly upregulated by ~10-fold, 3...
October 2017: Experimental and Therapeutic Medicine
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