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embryotic stem cell

Yanjie Guo, Tong Yu, Lei Lei, Anqin Duan, Xiaoling Ma, Huayan Wang
Direct reprogramming is an efficient strategy to convert one cell type to another. In this study, due to the failure of maintaining the undifferentiated state of goat embryotic stem- and induced pluripotent stem-like cells in vitro, we explored an alternative way to directly convert goat fibroblasts to lineage-specific cells. The 'Yamanaka factors' was ectopically expressed in fibroblasts for a short term to situate cells in a metastable state. By culturing with lineage-specific media for 1-2 weeks, the cardiomyocyte-like cells and neurocyte-like cells were generated and confirmed by the quantitative RT-PCR and immunocytochemical staining...
September 15, 2016: Animal Science Journal, Nihon Chikusan Gakkaihō
Yafeng Zhu, Lina Hultin-Rosenberg, Jenny Forshed, Rui M M Branca, Lukas M Orre, Janne Lehtiö
Alternative splicing is a pervasive process in eukaryotic organisms. More than 90% of human genes have alternatively spliced products, and aberrant splicing has been shown to be associated with many diseases. Current methods employed in the detection of splice variants include prediction by clustering of expressed sequence tags, exon microarray, and mRNA sequencing, all methods focusing on RNA-level information. There is a lack of tools for analyzing splice variants at the protein level. Here, we present SpliceVista, a tool for splice variant identification and visualization based on mass spectrometry proteomics data...
June 2014: Molecular & Cellular Proteomics: MCP
Hajime Inoue, Mitsuko Takenaga, Yuki Ohta, Miyuki Tomioka, Yu-Ichi Watabe, Masaki Aihara, Norio Kumagai
Somatic (adult) stem cells are thought to have pluripotency, just as do embryotic stem (ES) cells. We investigated the possibility that grafted epithelial keratinocytes could induce spinal cord regeneration in an animal model of spinal cord injury (SCI). Normal human keratinocytes were cultured by the routine technique, and normal human dermal fibroblasts were cultured by a similar method as a control group. SCI model was prepared by dropping a 10-g weight onto the exposed spinal cord of rats from a height of 25 mm, and 8 days later, the cultured cells were grafted into the injury site...
December 2011: Journal of Artificial Organs: the Official Journal of the Japanese Society for Artificial Organs
Christian Schwabe
Evolution is a complex problem by any standard. In an effort to fit the past events to the prevailing theory of evolution, the natural phenomenon has become so convoluted and polemic-ridden that it has floated clear out of the roam of science. This assay is an attempt to bring all phases of evolution of life in harmony with evidence derived from the fossil record. When a large number of organisms are found all over the earth within a relatively narrow time limit then life had many origins. A large number of different organisms appearing in their final form with all appendages functioning means that all development had occurred before the animals broke ground...
June 1, 2008: Cell Cycle
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