keyword
MENU ▼
Read by QxMD icon Read
search

protein interaction

keyword
https://www.readbyqxmd.com/read/29908468/first-report-on-the-pharmacokinetic-profile-of-nimbolide-a-novel-anticancer-agent-in-oral-and-intravenous-administrated-rats-by-lc-ms-method
#1
Shandilya Mahamuni Baira, Amit Khurana, Jaganmohan Somagoni, R Srinivas, Chandraiah Godugu, M V N Kumar Talluri
Nimbolide is a novel, natural compound with promising potential as a drug candidate for anticancer activity. It is isolated from the Indian traditional medicinal plant Azadirachta indica popularly known as neem. The present study was undertaken to explore the oral bioavailability and pharmacokinetic characteristics of nimbolide in rats using the LC/QTOF/MS method. A simple protein precipitation method using acetonitrile was employed for extracting nimbolide from rat plasma. The chromatographic separation of nimbolide and the internal standard (regorafenib) was attained on an Aquity BEH C18 column (100 × 2...
June 2, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29908451/inhibitors-of-protein-protein-interactions-ppis-an-analysis-of-scaffold-choices-and-buried-surface-area
#2
REVIEW
Xu Ran, Jason E Gestwicki
Protein-protein interactions (PPI) were once considered 'undruggable', but clinical successes, driven by advanced methods in drug discovery, have challenged that notion. Here, we review the last three years of literature on PPI inhibitors to understand what is working and why. From the 66 recently reported PPI inhibitors, we found that the average molecular weight was significantly greater than 500Da, but that this trend was driven, in large part, by the contribution of peptide-based compounds. Despite differences in average molecular weight, we found that compounds based on small molecules or peptides were almost equally likely to be potent inhibitors (KD <1μM)...
June 13, 2018: Current Opinion in Chemical Biology
https://www.readbyqxmd.com/read/29908440/organometallic-compounds-in-the-discovery-of-new-agents-against-kinetoplastid-caused-diseases
#3
REVIEW
Mauro Ravera, Elsa Moreno-Viguri, Rocio Paucar, Silvia Pérez-Silanes, Elisabetta Gabano
The development of safe and affordable antiparasitic agents effective against neglected tropical diseases is a big challenge of the drug discovery. The drugs currently employed have limitations such as poor efficacy, drug resistance or side effects. Thus, the search for new promising drugs is more and more crucial. Metal complexes and, in particular, organometallic compounds may expand the list of the drug candidates due to the peculiar attributes that the presence of the metal core add to the organic fragment (e...
June 1, 2018: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29908372/association-of-fibrotic-remodeling-and-cytokines-chemokines-content-in-epicardial-adipose-tissue-with-atrial-myocardial-fibrosis-in-patients-with-atrial-fibrillation
#4
Ichitaro Abe, Yasushi Teshima, Hidekazu Kondo, Haruka Kaku, Shintaro Kira, Yuki Ikebe, Shotaro Saito, Akira Fukui, Tetsuji Shinohara, Kunio Yufu, Mikiko Nakagawa, Naoki Hijiya, Masatsugu Moriyama, Tatsuo Shimada, Shinji Miyamoto, Naohiko Takahashi
BACKGROUND: Epicardial adipose tissue (EAT) is associated with atrial fibrillation (AF), but the underlying mechanisms remain to be fully elucidated. OBJECTIVE: Using human left atrial appendage (LAA) samples, we examined the interactive relationship between the EAT profile and atrial myocardial fibrosis through histological and biochemical analyses. METHODS: LAA samples were obtained from 59 consecutive AF patients during cardiovascular surgery...
June 13, 2018: Heart Rhythm: the Official Journal of the Heart Rhythm Society
https://www.readbyqxmd.com/read/29908302/inhibitory-effect-of-pxr-on-ammonia-induced-hepatocyte-autophagy-via-p53
#5
Linlin Yan, Zhanfei Chen, Luxi Wu, Yongfa Su, Xiaoqian Wang, Nanhong Tang
Pregnane X Receptor (PXR), a nuclear receptor transcription factor, participates in a wide range of physiological activities, but the regulation of ammonia-induced hepatocyte autophagy by PXR is not yet clear. In this study, the levels of down-regulated LC3B-II and up-regulated SQSTM1 were found in ammonia-induced PXR-overexpressing (PXR+) liver cells, but the opposite appeared in PXR-knockdown (PXR-) liver cells. Rifampicin, a PXR-activating agent, elicits a similar effect as PXR + cells. The mechanism analysis reveals that the levels of the energy-sensitive molecule AMPKβ1 and phosphorylated AMPKβ1 (p-AMPKβ1) in PXR - cells are higher than those in control cells, whereas the levels of this molecule in PXR + cells are lower than those in control cells...
June 13, 2018: Toxicology Letters
https://www.readbyqxmd.com/read/29908287/characterization-of-nanomedicines-surface-coverage-using-molecular-probes-and-capillary-electrophoresis
#6
J-B Coty, F Varenne, A Benmalek, O Garsaa, I Le Potier, M Taverna, C Smadja, C Vauthier
A faithful characterization of nanomedicine (NM) is needed for a better understanding of their in vivo outcomes. Size and surface charge are studied with well-established methods. However, other relevant parameters for the understanding of NM behavior in vivo remain largely inaccessible. For instance, the reactive surface of nanomedicines, which are often grafted with macromolecules to decrease their recognition by the immune system, is excluded from a systematic characterization. Yet, it is known that a subtle modification of NM's surface characteristics (grafting density, molecular architecture and conformation of macromolecules) is at the root of major changes in the presence of biological components...
June 13, 2018: European Journal of Pharmaceutics and Biopharmaceutics
https://www.readbyqxmd.com/read/29908275/role-of-modification-route-for-zinc-oxide-nanoparticles-on-protein-structure-and-their-effects-on-glioblastoma-cells
#7
Mine Altunbek, Seda Keleştemur, Gülin Baran, Mustafa Çulha
Zinc oxide nanoparticles (ZnO) are presented as potential cancer therapeutic agent based on their surface properties. In this study, the most abundant blood proteins, albumin, fibrinogen and apo-transferrin, were covalently bound (c-ZnO NPs) and nonspecifically adsorbed (n-ZnO NPs) onto ZnO NPs to evaluate the role of modification route on protein structure and their effects on glioblastoma cells. The success of modification and structures of proteins on ZnO NPs were characterized with FT-IR. It was found that non-covalent interaction significantly damaged the secondary structure of proteins compared to those covalently attached to the ZnO nanoparticle...
June 13, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29908232/the-association-of-spinophilin-with-disks-large-associated-protein-3-sapap3-is-regulated-by-metabotropic-glutamate-receptor-mglur-5
#8
Cameron W Morris, Darryl S Watkins, Asma B Salek, Michael C Edler, Anthony J Baucum
Spinophilin is the most abundant protein phosphatase 1 targeting protein in the postsynaptic density of dendritic spines. Spinophilin associates with myriad synaptic proteins to regulate normal synaptic communication; however, the full complement of spinophilin interacting proteins and mechanisms regulating spinophilin interactions are unclear. Here we validate an association between spinophilin and the scaffolding protein, disks large-associated protein 3 (SAP90/PSD-95 associated protein 3; SAPAP3). Loss of SAPAP3 leads to obsessive-compulsive disorder (OCD)-like behaviors due to alterations in metabotropic glutamate receptor (mGluR) signaling...
June 13, 2018: Molecular and Cellular Neurosciences
https://www.readbyqxmd.com/read/29908182/a-site-specific-branching-poly-glutamate-tag-mediates-intracellular-protein-delivery-by-cationic-lipids
#9
Si-Qi Huang, Bei-Bei Han, Yan-Mei Li, Yong-Xiang Chen
Intracellular protein delivery is of significance for cellular protein analysis and therapeutic development, but remains challenging technically. Herein, we report a general and highly potent strategy for intracellular protein delivery based on commercially available cationic lipids. In this strategy, a designed double branching poly-glutamate tag is site-specifically attached onto the C-terminal of protein cargos via expressed protein ligation (EPL), which mediates the entrapment of proteins into cationic liposomes driven by electrostatic interaction...
June 13, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29908138/interaction-of-pirenzepine-with-bovine-serum-albumin-and-effect-of-%C3%AE-cyclodextrin-on-binding-a-biophysical-and-molecular-docking-approach
#10
Yusra Rahman, Shumaila Afrin, Mohammad Tabish
Studying the interaction of therapeutic molecules with serum albumin is important to understand their biopharmaceutics, pharmacokinetics and toxicity as well as their relation with the structure and function of protein. The biomolecular interaction of an anti-spasmodic drug, pirenzepine with bovine serum albumin (BSA) was investigated using multi-spectroscopic, calorimetric and docking studies. Fluorescence quenching of BSA on interaction with pirenzepine revealed the static mode of quenching. Pirenzepine exhibited a moderate binding to serum albumin with the binding constant value in the order of 104  M-1 ...
June 13, 2018: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29908124/modifications-in-small-nuclear-rnas-and-their-roles-in-spliceosome-assembly-and-function
#11
Markus T Bohnsack, Katherine E Sloan
Modifications in cellular RNAs have emerged as key regulators of all aspects of gene expression, including pre-mRNA splicing. During spliceosome assembly and function, the small nuclear RNAs (snRNAs) form numerous dynamic RNA-RNA and RNA-protein interactions, which are required for spliceosome assembly, correct positioning of the spliceosome on substrate pre-mRNAs and catalysis. The human snRNAs contain several base methylations as well as a myriad of pseudouridines and 2'-O-methylated nucleotides, which are largely introduced by small Cajal body-specific-RNPs...
June 1, 2018: Biological Chemistry
https://www.readbyqxmd.com/read/29908072/glycyrrhizin-has-a-high-likelihood-to-be-a-victim-of-drug-drug-interactions-mediated-by-hepatic-oatp1b1-1b3
#12
Jiajia Dong, Olajide E Olaleye, Rongrong Jiang, Jing Li, Chuang Lu, Feifei Du, Fang Xu, Junling Yang, Fengqing Wang, Weiwei Jia, Chuan Li
BACKGROUND AND PURPOSE: Intravenous glycyrrhizin, having anti-inflammatory and hepatoprotective properties, is incorporated into the management of liver diseases in China. This investigation was designed to elucidate the molecular mechanism underlying hepatobiliary excretion of glycyrrhizin and to investigate its potential for drug-drug interactions on organic anion-transporting polypeptides (OATP)1B. EXPERIMENTAL APPROACH: Human hepatic transporters were characterized for glycyrrhizin at the cellular and vesicular levels and compared with rat hepatic transporters...
June 16, 2018: British Journal of Pharmacology
https://www.readbyqxmd.com/read/29908065/a-complex-interplay-between-the-extracellular-matrix-and-the-innate-immune-response-to-microbial-pathogens
#13
REVIEW
Hannah Tomlin, Anna Maria Piccinini
The role of the host extracellular matrix (ECM) in infection tends to be neglected. However, the complex interactions between invading pathogens, host tissues and immune cells occur in the context of the ECM. On the pathogen side, a variety of surface and secreted molecules, including microbial surface components recognizing adhesive matrix molecules (MSCRAMM) and tissue degrading enzymes, are employed that interact with different ECM proteins in order to effectively establish an infection at specific sites...
June 16, 2018: Immunology
https://www.readbyqxmd.com/read/29908064/unconventional-rna-binding-proteins-an-uncharted-zone-in-rna-biology
#14
REVIEW
Waleed S Albihlal, André P Gerber
RNA-binding proteins play essential roles in the post-transcriptional regulation of gene expression. While hundreds of RNA-binding proteins can be predicted computationally, the recent introduction of proteome-wide approaches has dramatically expanded the repertoire of proteins interacting with RNA. Besides canonical RNA-binding proteins that contain characteristic RNA-binding domains, many proteins that lack such domains but have other well-characterised cellular functions were identified; including metabolic enzymes, heat shock proteins, kinases, as well as transcription factors and chromatin-associated proteins...
June 16, 2018: FEBS Letters
https://www.readbyqxmd.com/read/29907954/inter-relationships-between-the-heterotrimeric-g%C3%AE-subunit-agb1-the-rlk-feronia-and-ralf1-in-salinity-response
#15
Yunqing Yu, Sarah M Assmann
Plant heterotrimeric G proteins modulate numerous developmental stress responses. Recently, receptor-like kinases (RLKs) have been implicated as functioning with G proteins, and may serve as plant G-protein-coupled-receptors (GPCRs). The RLK FERONIA (FER), in the Catharantus roseus RLK1-like subfamily, is activated by a family of polypeptides called Rapid Alkalinization Factors (RALFs). We previously showed that the Arabidopsis G protein β subunit, AGB1, physically interacts with FER, and that RALF1 regulation of stomatal movement through FER requires AGB1...
June 15, 2018: Plant, Cell & Environment
https://www.readbyqxmd.com/read/29907875/a-novel-homozygous-missense-mutation-in-the-sh3-binding-motif-of-stambp-causing-microcephaly-capillary-malformation-syndrome
#16
Ikumi Hori, Fuyuki Miya, Yutaka Negishi, Ayako Hattori, Naoki Ando, Keith A Boroevich, Nobuhiko Okamoto, Mitsuhiro Kato, Tatsuhiko Tsunoda, Mami Yamasaki, Yonehiro Kanemura, Kenjiro Kosaki, Shinji Saitoh
Microcephaly-capillary malformation syndrome is a congenital and neurodevelopmental disorder caused by biallelic mutations in the STAMBP gene. Here we identify the novel homozygous mutation located in the SH3 binding motif of STAMBP (NM_006463.4) (c.707C>T: p.Ser236Phe) through whole-exome sequencing. The case patient was a 2-year-old boy showing severe global developmental delay, progressive microcephaly, refractory seizures, dysmorphic facial features, and multiple capillary malformations. Immunoblot analysis of patient-derived lymphoblastoid cell lines (LCLs) revealed a severe reduction in STAMBP expression, indicating that Ser236Phe induces protein instability...
June 15, 2018: Journal of Human Genetics
https://www.readbyqxmd.com/read/29907748/extracellular-nanovesicles-released-from-the-commensal-yeast-malassezia-sympodialis-are-enriched-in-allergens-and-interact-with-cells-in-human-skin
#17
Henrik J Johansson, Helen Vallhov, Tina Holm, Ulf Gehrmann, Anna Andersson, Catharina Johansson, Hans Blom, Marta Carroni, Janne Lehtiö, Annika Scheynius
Malassezia sympodialis is a dominant commensal fungi in the human skin mycobiome but is also associated with common skin disorders including atopic eczema (AE). M. sympodialis releases extracellular vesicles, designated MalaEx, which are carriers of small RNAs and allergens, and they can induce inflammatory cytokine responses. Here we explored how MalaEx are involved in host-microbe interactions by comparing protein content of MalaEx with that of the parental yeast cells, and by investigating interactions of MalaEx with cells in the skin...
June 15, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29907735/identification-of-proteins-potentially-associated-with-renal-aging-in-rats
#18
Diangeng Li, Delong Zhao, Weiguang Zhang, Qian Ma, Dong Liu, Qi Huang, Ying Zheng, Xueyuan Bai, Xuefeng Sun, Xiangmei Chen
We established a young (Y)-old (O) rat kidney transplantation model. With this model, we detected no age-related differences in renal structure between Y→Y and Y→O kidneys or O→O and O→Y kidneys. However, we did detect differences in levels of the senescence markers β-gal and p16 as well as the inflammatory cytokines TNF-α and IL-1β. Using proteomics analysis we detected 66 proteins associated with suppression of aging and 73 proteins associated with enhancement of aging. After construction of a protein-protein interaction network, a total of 73 nodes and 99 edges were analyzed using MCODE, and three significant modules were selected...
June 14, 2018: Aging
https://www.readbyqxmd.com/read/29907707/-leishmania-donovani-exploits-tollip-a-multitasking-protein-to-impair-tlr-il-1r-signaling-for-its-survival-in-the-host
#19
Naveen Parmar, Pragya Chandrakar, Preeti Vishwakarma, Kavita Singh, Kalyan Mitra, Susanta Kar
IL-1R/TLR signaling plays a significant role in sensing harmful foreign pathogens and mounting effective innate and adaptive immune responses. However, the precise mechanism by which Leishmania donovani, an obligate intramacrophagic pathogen, breaches IL-1R/TLR signaling and host-protective immunity remains obscure. In this study, we report the novel biphasic role of Toll-interacting protein (Tollip), a negative regulator of the IL-1R/TLR pathway, in the disease progression of experimental visceral leishmaniasis...
June 15, 2018: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/29907651/lncrna-papas-tethered-to-the-rdna-enhancer-recruits-hypophosphorylated-chd4-nurd-to-repress-rrna-synthesis-at-elevated-temperatures
#20
Zhongliang Zhao, Nevcin Sentürk, Chenlin Song, Ingrid Grummt
Attenuation of pre-rRNA synthesis in response to elevated temperature is accompanied by increased levels of PAPAS ("promoter and pre-rRNA antisense") , a long noncoding RNA (lncRNA) that is transcribed in an orientation antisense to pre-rRNA. Here we show that PAPAS interacts directly with DNA, forming a DNA-RNA triplex structure that tethers PAPAS to a stretch of purines within the enhancer region, thereby guiding associated CHD4/NuRD (nucleosome remodeling and deacetylation) to the rDNA promoter...
June 15, 2018: Genes & Development
keyword
keyword
101565
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"