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https://www.readbyqxmd.com/read/29668051/evidence-for-a-specific-and-critical-role-of-mitogen-activated-protein-kinase-20-in-uni-to-binucleate-transition-of-microgametogenesis-in-tomato
#1
Lifei Chen, Dandan Yang, Youwei Zhang, Limin Wu, Yaoyao Zhang, Lei Ye, Changtian Pan, Yanjun He, Li Huang, Yong-Ling Ruan, Gang Lu
Mitogen-activated protein kinases (MAPKs) regulate diverse aspects of plant growth. However, their potential role in reproductive development remains elusive. Here, we discovered an unique role of SlMPK20, a plant-specific group D MAPK, in pollen development in tomato. RNAi-mediated suppression of SlMPK20 or its knockout using CRISPR/Cas9 significantly reduced or completely abolished pollen viability, respectively, with no effects on maternal fertility. Cell biology and gene expression analyses established that SlMPK20 exerts its role specifically at the uni-to-binucleate transition during microgametogenesis...
April 18, 2018: New Phytologist
https://www.readbyqxmd.com/read/29668012/evolution-of-dnmt2-in-drosophilids-evidence-for-positive-and-purifying-selection-and-insights-into-new-protein-pathways-interactions
#2
Gilberto Cavalheiro Vieira, Marícia Fantinel D'Ávila, Rebeca Zanini, Maríndia Deprá, Vera Lúcia da Silva Valente
The DNA methyltransferase 2 (DNMT2) protein is the most conserved member of the DNA methyltransferase family. Nevertheless, its substrate specificity is still controversial and elusive. The genomic role and determinants of DNA methylation are poorly understood in invertebrates, and several mechanisms and associations are suggested. In Drosophila, the only known DNMT gene is Dnmt2. Here we present our findings from a wide search for Dnmt2 homologs in 68 species of Drosophilidae. We investigated its molecular evolution, and in our phylogenetic analyses the main clades of Drosophilidae species were recovered...
2018: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/29667836/molecular-dynamics-simulations-are-redefining-our-view-of-peptides-interacting-with-biological-membranes
#3
Jakob P Ulmschneider, Martin B Ulmschneider
Ever since the first molecular mechanics computer simulations of biological molecules became possible, there has been the dream to study all complex biological phenomena in silico, simply bypassing the enormous experimental challenges and their associated costs. For this, two inherent requirements need to be met: First, the time scales achievable in simulations must reach up to the millisecond range and even longer. Second, the computational model must accurately reproduce what is measured experimentally. Despite some recent successes, the general consensus in the field to date has been that neither of these conditions have yet been met and that the dream will be realized, if at all, only in the distant future...
April 18, 2018: Accounts of Chemical Research
https://www.readbyqxmd.com/read/29667761/plant-glycosylphosphatidylinositol-gpi-anchored-proteins-at-the-plasma-membrane-cell-wall-nexus
#4
REVIEW
Trevor H Yeats, Antony Bacic, Kim L Johnson
Approximately 1% of plant proteins are predicted to be post-translationally modified with a glycosylphosphatidylinositol (GPI) anchor that tethers the polypeptide to the outer leaflet of the plasma membrane. While the synthesis and structure of GPI anchors is largely conserved across eukaryotes, the repertoire of functional domains present in the GPI-anchored proteome has diverged substantially. In plants, this includes a large fraction of the GPI-anchored proteome being further modified with plant-specific arabinogalactan (AG) O-glycans...
April 18, 2018: Journal of Integrative Plant Biology
https://www.readbyqxmd.com/read/29667747/self-assembled-cyclic-structures-from-copper-ii-peptoids
#5
Totan Ghosh, Natalia Fridman, Monica Kosa, Galia Maayan
Metal-ligand coordination is a key interaction in the self-assembly of biopolymers. Although binding of metal ions to synthetic proteins and peptides is known to yield high-order structures, self-assembly of peptidomimetic molecules upon metal binding is still challenging. Herein we explore the self-assembly of three peptoid trimers 3, 3A and 3B, bearing a bipyridine ligand at their C-terminus, a benzyl group at their N-terminus and a polar group (N-ethyl-R) in the middle position (R = OH, OCH3 and NH2, respectively) by Cu2+ coordination...
April 18, 2018: Angewandte Chemie
https://www.readbyqxmd.com/read/29667660/a-new-functional-membrane-protein-microarray-based-on-tethered-phospholipid-bilayers
#6
Meriem Chadli, Ofelia Maniti, Christophe Marquette, Bruno Tillier, Sandra Cortès, Agnès Girard-Egrot
A new prototype of a membrane protein biochip is presented in this article. This biochip was created by the combination of novel technologies of peptide-tethered bilayer lipid membrane (pep-tBLM) formation and solid support micropatterning. Pep-tBLMs integrating a membrane protein were obtained in the form of microarrays on a gold chip. The formation of the microspots was visualized in real-time by surface plasmon resonance imaging (SPRi) and the functionality of a GPCR (CXCR4), reinserted locally into microwells, was assessed by ligand binding studies...
April 18, 2018: Analyst
https://www.readbyqxmd.com/read/29667549/dentification-and-quantification-of-quercetin-a-major-consitituent-of-artocarpus-altilis-by-targeting-related-genes-of-apoptosis-and-cell-cycle-in-vitro-cytotoxic-activity-against-human-lung-carcinoma-cell-lines-a549
#7
Silvana Russo, Walter Filgueira De Azevedo
BACKGROUND: Cannabinoid Receptor 1 (CB1) is a membrane protein prevalent in the central nervous system, whose crystallographic structure has recently been solved. Studies will be needed to investigate CB1 complexes with its ligands and its role in the development of new drugs. OBJECTIVE: Our goal here is to review the studies on CB1, starting with general aspects and focusing on the recent structural studies, with emphasis on the inverse agonists bound structures...
April 17, 2018: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/29667536/simulation-of-protein-diffusion-a-sensitive-probe-of-protein-solvent-interactions
#8
Valerio Ferrario, Jürgen Pleiss
Aqueous solutions of Candida antarctica lipase B (CALB) were simulated considering three different water models (SPC/E, TIP3P, TIP4P) by a series of molecular dynamics (MD) simulations of three different box sizes (L = 9, 14, and 19 nm) to determine the diffusion coefficient, the water viscosity, and the protein density. The protein-water systems were equilibrated for 500 ns, followed by 100 ns production runs which were analyzed. The diffusional properties of CALB were characterized by the Stokes radius (RS ), which was derived from the diffusion coefficient and the viscosity...
April 18, 2018: Journal of Biomolecular Structure & Dynamics
https://www.readbyqxmd.com/read/29667486/lncrna-adpgk-as1-promotes-pancreatic-cancer-progression-through-activating-zeb1-mediated-epithelial-mesenchymal-transition
#9
Suzhen Song, Weihua Yu, Sen Lin, Mingbao Zhang, Teng Wang, Shuang Guo, Hongbo Wang
OBJECTIVE: This study was conducted to investigate the effects of ADP dependent glucokinase antisense RNA 1 (ADPGK-AS1)/ miR-205-5p/ zinc finger E-box binding homeobox 1 (ZEB1) on PC cells. METHODS: Differentially expressed lncRNAs and miRNAs in pancreatic cancer (PC) were identified by microarray analysis. In silico ceRNA analysis was conducted to find out the interactions among lncRNAs, miRNAs and mRNAs. Quantitative real-time PCR (qRT-PCR) was utilized to examine the expression of miR-205-5p and lncRNA ADPGK-AS1 in PC and non-cancerous cells...
April 18, 2018: Cancer Biology & Therapy
https://www.readbyqxmd.com/read/29667327/novel-west-syndrome-candidate-genes-in-a-chinese-cohort
#10
Jing Peng, Ying Wang, Fang He, Chen Chen, Li-Wen Wu, Li-Fen Yang, Yu-Ping Ma, Wen Zhang, Zi-Qing Shi, Chao Chen, Kun Xia, Hui Guo, Fei Yin, Nan Pang
AIMS: West syndrome (WS) is a classic form of early infantile epileptic encephalopathy (EIEE) characterized by tonic spasms with clustering, arrest of psychomotor development, and hypsarrhythmia on electroencephalography. Genetic defects play a critical role in the pathology of WS, and 54 EIEE genes have been identified till date. This study was designed to uncover new candidate genes for West syndrome. METHODS: In this study, we recruited 56 Chinese families with WS of unknown etiology...
April 17, 2018: CNS Neuroscience & Therapeutics
https://www.readbyqxmd.com/read/29667320/flotillin-1-interacts-with-the-serotonin-transporter-and-modulates-chronic-corticosterone-response
#11
Sonali N Reisinger, Eryan Kong, Barbara Molz, Thomas Humberg, Spyros Sideromenos, Ana Cicvaric, Thomas Steinkellner, Jae-Won Yang, Maureen Cabatic, Francisco J Monje, Harald H Sitte, Benjamin J Nichols, Daniela D Pollak
Aberrant serotonergic neurotransmission in the brain is considered at the core of the pathophysiological mechanisms involved in neuropsychiatric disorders. Gene by environment interactions contribute to the development of depression and involve modulation of the availability and functional activity of the serotonin transporter (SERT). Using behavioral and in vivo electrophysiological approaches together with biochemical, molecular-biological and molecular imaging tools we establish Flotillin-1 (Flot1) as a novel protein interacting with SERT and demonstrate its involvement in the response to chronic corticosterone (CORT) treatment...
April 18, 2018: Genes, Brain, and Behavior
https://www.readbyqxmd.com/read/29667268/a-molecular-network-for-functional-versatility-of-hecate-transcription-factors
#12
Christophe Gaillochet, Suraj Jamge, Froukje van der Wal, Gerco Angenent, Richard Immink, Jan U Lohmann
During the plant life cycle, diverse signalling inputs are continuously integrated and engage specific genetic programs depending on the cellular or developmental context. Consistent with an important role in this process, HECATE (HEC) bHLH transcription factors display diverse functions, from photomorphogenesis to the control of shoot meristem dynamics and gynoecium patterning. However, the molecular mechanisms underlying their functional versatility and the deployment of specific HEC sub-programs still remain elusive...
April 18, 2018: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/29667261/apple-s-rnase-interacts-with-an-actin-binding-protein-mdmvg-to-reduce-pollen-tube-growth-by-inhibiting-its-actin-severing-activity-at-the-early-stage-of-self-pollination-induction
#13
Qing Yang, Dong Meng, Zhaoyu Gu, Wei Li, Qiuju Chen, Yang Li, Hui Yuan, Jie Yu, Chunsheng Liu, Tianzhong Li
In S-RNase-mediated self-incompatibility (SI), S-RNase secreted from the style destroys the actin cytoskeleton of the self-pollen tubes, eventually halting its growth, but the mechanism remains unclear. In vitro biochemical assays revealed that S-RNase does not bind or sever filamentous actin (F-actin). In apple (Malus×domestica), we identified an actin-binding protein containing myosin, villin and GRAM, MdMVG, that physically interacts with S-RNase and directly binds and severs F-actin. Immunofluorescence assays and total internal reflection fluorescence microscopy (TIRFM) indicated that S-RNase inhibits the F-actin-severing activity of MdMVG in vitro...
April 18, 2018: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/29667247/the-nuclear-factor-constans-complex-antagonizes-polycomb-repression-to-de-repress-flowering-locus-t-expression-in-response-to-inductive-long-days-in-arabidopsis
#14
Xiao Luo, Zheng Gao, Yizhong Wang, Zhijuan Chen, Wenju Zhang, Jirong Huang, Hao Yu, Yuehui He
Many plants sense the seasonal cues, day length or photoperiod changes, to align the timing of the developmental transition to flowering with changing seasons for reproductive success. Inductive day lengths through the photoperiod pathway induces the expression of FLOWERING LOCUS T (FT) or FT relatives that encode a major mobile florigen to promote flowering. In Arabidopsis thaliana, under inductive long days the photoperiod pathway output CONSTANS (CO) accumulates toward the end of the day and associates with the B and C subunits of Nuclear Factor Y (NF-Y) to form the NF-CO complex that acts to promote FT expression near dusk, whereas Polycomb group (PcG) proteins function to silence FT expression...
April 18, 2018: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/29667162/metaunidec-high-throughput-deconvolution-of-native-mass-spectra
#15
Deseree J Reid, Jessica M Diesing, Matthew A Miller, Scott M Perry, Jessica A Wales, William R Montfort, Michael T Marty
The expansion of native mass spectrometry (MS) methods for both academic and industrial applications has created a substantial need for analysis of large native MS datasets. Existing software tools are poorly suited for high-throughput deconvolution of native electrospray mass spectra from intact proteins and protein complexes. The UniDec Bayesian deconvolution algorithm is uniquely well suited for high-throughput analysis due to its speed and robustness but was previously tailored towards individual spectra...
April 17, 2018: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/29667121/backbone-1-h-13-c-and-15-n-resonance-assignments-of-the-ligand-binding-domain-of-the-human-wildtype-glucocorticoid-receptor-and-the-f602s-mutant-variant
#16
Christian Köhler, Göran Carlström, Stefan Tångefjord, Tineke Papavoine, Matti Lepistö, Karl Edman, Mikael Akke
The glucocorticoid receptor (GR) is a nuclear hormone receptor that regulates key genes controlling development, metabolism, and the immune response. GR agonists are efficacious for treatment of inflammatory, allergic, and immunological disorders. Steroid hormone binding to the ligand-binding domain (LBD) of GR is known to change the structural and dynamical properties of the receptor, which in turn control its interactions with DNA and various co-regulators and drive the pharmacological response. Previous biophysical studies of the GR LBD have required the use of mutant forms to overcome issues with limited protein stability and high aggregation propensity...
April 17, 2018: Biomolecular NMR Assignments
https://www.readbyqxmd.com/read/29667047/investigation-of-the-mechanism-of-therapeutic-protein-drug-interaction-between-methotrexate-and-golimumab-an-anti-tnf%C3%AE-monoclonal-antibody
#17
Weirong Wang, Jocelyn Leu, Rebecca Watson, Zhenhua Xu, Honghui Zhou
A prominent example of human therapeutic protein-drug interaction (TP-DI) is between methotrexate (MTX) and anti-TNFα mAbs. One plausible mechanism for this TP-DI is through the pharmacodynamic effect of MTX on immunogenicity. However, there is no definitive evidence to substantiate this mechanism, and other competing hypotheses, such as MTX suppressing FcγRI expression thereby affecting mAb PK, have also been proposed. In order to understand this mechanism, a cynomolgus monkey study was conducted using golimumab as a model compound...
April 17, 2018: AAPS Journal
https://www.readbyqxmd.com/read/29667001/c-phycoerythrin-as-a-colorimetric-and-fluorometric-probe-for-the-sensitive-selective-and-quantitative-detection-of-cu-2-in-aqueous-samples
#18
Tonmoy Ghosh, Apeksha Vyas, Khushbu Bhayani, Sandhya Mishra
C-phycoerythrin (CPE) was investigated as a colorimetric and fluorometric quantitative sensor for Cu2+ ions in an aqueous medium. UV - visible studies with 50 μM concentration of different metals were carried out with only Cu and Ag showing changes in the absorption spectra. Fluorescence emission studies showed similar results. UV - visible titration of CPE with different [Cu] resulted in a linear relationship within 10 μM Cu and a 'naked eye' visible difference in colour, most likely due to the formation of a CPE - Cu complex...
April 18, 2018: Journal of Fluorescence
https://www.readbyqxmd.com/read/29666914/improved-lc-ms-ms-method-for-the-quantification-of-hepcidin-25-in-clinical-samples
#19
Ioana M Abbas, Holger Hoffmann, María Montes-Bayón, Michael G Weller
Mass spectrometry-based methods play a crucial role in the quantification of the main iron metabolism regulator hepcidin by singling out the bioactive 25-residue peptide from the other naturally occurring N-truncated isoforms (hepcidin-20, -22, -24), which seem to be inactive in iron homeostasis. However, several difficulties arise in the MS analysis of hepcidin due to the "sticky" character of the peptide and the lack of suitable standards. Here, we propose the use of amino- and fluoro-silanized autosampler vials to reduce hepcidin interaction to laboratory glassware surfaces after testing several types of vials for the preparation of stock solutions and serum samples for isotope dilution liquid chromatography-tandem mass spectrometry (ID-LC-MS/MS)...
April 18, 2018: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29666868/in-vitro-antibacterial-activity-of-oxide-and-non-oxide-bioceramics-for-arthroplastic-devices-ii-fourier-transform-infrared-spectroscopy
#20
Francesco Boschetto, Nami Toyama, Satoshi Horiguchi, Ryan M Bock, Bryan J McEntire, Tetsuya Adachi, Elia Marin, Wenliang Zhu, Osam Mazda, B Sonny Bal, Giuseppe Pezzotti
The metabolic response of Gram-positive Staphylococcus epidermidis (S. epidermidis) bacteria to bioceramic substrates was probed by means of Fourier transform infrared spectroscopy (FTIR). Oxide zirconia-toughened alumina (ZTA) and non-oxide silicon nitride (Si3N4) substrates were tested. Bacteria exposed to silica glass substrates were used as a control. S. epidermidis, a major cause of periprosthetic infections, was screened to obtain a precise time-lapse knowledge of its molecular composition and to mechanistically understand its interaction with different substrates...
April 18, 2018: Analyst
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