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https://www.readbyqxmd.com/read/28165250/programmable-phase-transitions-in-a-photonic-microgel-system-linking-soft-interactions-to-a-temporal-ph-gradient
#1
Dennis Go, Dirk Rommel, Lisa Chen, Feng Shi, Joris Sprakel, Alexander J C Kuehne
Soft amphoteric microgel systems exhibit a rich phase behavior. Crystalline phases of these material systems are of interest because they exhibit photonic stop-gaps, giving rise to iridescent color. Such microgel systems are promising for applications in soft, switchable and programmable photonic filters and devices. We here report a composite microgel system constisting of a hard and fluorescently labeled core and a soft, amphoteric microgel shell. At pH above the isoelectric point (IEP), these colloids easily crystallize into three-dimensional colloidal assemblies...
February 6, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28079383/structure-and-interaction-in-the-ph-dependent-phase-behavior-of-nanoparticle-protein-systems
#2
Indresh Yadav, Sugam Kumar, Vinod K Aswal, Joachim Kohlbrecher
The pH-dependent structure and interaction of anionic silica nanoparticles (diameter 18 nm) with two globular model proteins, lysozyme and bovine serum albumin (BSA), have been studied. Cationic lysozyme adsorbs strongly on the nanoparticles, and the adsorption follows exponential growth as a function of lysozyme concentration, where the saturation value increases as pH approaches the isoelectric point (IEP) of lysozyme. By contrast, irrespective of pH, anionic BSA does not show any adsorption. Despite having a different nature of interactions, both proteins render a similar phase behavior where nanoparticle-protein systems transform from being one-phase (clear) to two-phase (turbid) above a critical protein concentration (CPC)...
January 23, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28075207/perceived-needs-for-different-components-in-a-rehabilitation-program-among-cancer-survivors-with-chronic-fatigue-compared-to-survivors-without-chronic-fatigue
#3
Gunhild M Gjerset, Jon H Loge, Cecilie E Kiserud, Sophie D Fosså, Sævar B Gudbergsson, Line M Oldervoll, Torbjørn Wisløff, Lene Thorsen
BACKGROUND: Knowledge about the user' needs is important to develop targeted rehabilitation for cancer patients with chronic fatigue (CF). The aims of the study were to examine prevalence of CF in cancer survivors attending an one-week inpatient educational program (IEP) and to identify characteristics of those with CF. Further to examine the perceived needs for different components in a rehabilitation program, need of complex rehabilitation (at least two components) and aspects of health-related quality of life (HRQoL) among survivors with CF versus those without CF...
January 11, 2017: Acta Oncologica
https://www.readbyqxmd.com/read/28069233/a-facile-sonochemical-synthesis-of-shell-stabilized-reactive-microbubbles-using-surface-thiolated-bovine-serum-albumin-with-the-traut-s-reagent
#4
Xiaochen Ma, Adrien Bussonniere, Qingxia Liu
The short lifetime of proteinaceous microbubbles produced using conventional sonication method has hindered their applications in drug delivery and metal removal from wastewater. In this study, we aimed to synthesize stable proteinaceous microbubbles and to demonstrate their reactivity. Our model protein, bovine serum albumin (BSA) was treated with 2-iminothiolane hydrochloride (Traut's reagent) to convert primary amines to thiols before the synthesis of microbubbles. Microbubbles produced with the Traut's reagent-treated BSA (BSA-SH MBs) were initially concentrated at median sizes of 0...
May 2017: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/28063520/zeta-potential-of-artificial-and-natural-calcite-in-aqueous-solution
#5
REVIEW
Dawoud Al Mahrouqi, Jan Vinogradov, Matthew D Jackson
Despite the broad range of interest and applications, controls on calcite surface charge in aqueous solution, especially at conditions relevant to natural systems, remain poorly understood. The primary data source to understand calcite surface charge comprises measurements of zeta potential. Here we collate and review previous measurements of zeta potential on natural and artificial calcite and carbonate as a resource for future studies, compare and contrast the results of these studies to determine key controls on zeta potential and where uncertainties remain, and report new measurements of zeta potential relevant to natural subsurface systems...
February 2017: Advances in Colloid and Interface Science
https://www.readbyqxmd.com/read/28041806/neurodevelopmental-exam-recommendations-for-children-with-hiv-infection
#6
Kathryn Brady, Madison Dipman, Erin Nelson, Megan Allen, Laura Clarke-Steffen, Amy Edmonds, Kiran Aurora, Janice Piatt
Multiple and complex health, social, and environmental factors threaten the school success of children living with HIV. Little is known about interventions to overcome these threats to school success. We aimed to identify the number and types of recommendations from hospital-generated neurodevelopmental exams and school-generated evaluations, Individual Education Plans (IEP), and 504 Plans for adaptations in the classroom for students with HIV infection. We also compared recommendations suggested by both neurodevelopmental exams and IEPs or 504 Plans...
November 19, 2016: Journal of the Association of Nurses in AIDS Care: JANAC
https://www.readbyqxmd.com/read/28025341/can-we-replace-curation-with-information-extraction-software
#7
REVIEW
Peter D Karp
Can we use programs for automated or semi-automated information extraction from scientific texts as practical alternatives to professional curation? I show that error rates of current information extraction programs are too high to replace professional curation today. Furthermore, current IEP programs extract single narrow slivers of information, such as individual protein interactions; they cannot extract the large breadth of information extracted by professional curators for databases such as EcoCyc. They also cannot arbitrate among conflicting statements in the literature as curators can...
2016: Database: the Journal of Biological Databases and Curation
https://www.readbyqxmd.com/read/27990469/attitude-towards-and-readiness-for-interprofessional-education-in-medical-and-nursing-students-of-bern
#8
Ulrich Woermann, Lena Weltsch, Alexandra Kunz, Daniel Stricker, Sissel Guttormsen
Objectives: Interprofessional collaboration is becoming increasingly important in health care for various reasons. Interprofessional Education (IPE) can provide a basis for this. The aim of our study was to find out how medical (MS) and nursing students (NS) think about their own and other professions, what they know about each other, how strong their willingness to embrace IPE is, and what forms of IPE they deem useful. Methodology: Seven IPE experts rated the two measuring instruments, Readiness for Interprofessional Learning Scale RIPLS, and Interdisciplinary Education Perception Scale IEPS in terms of relevance of the items, and the quality of translation into German...
2016: GMS Journal for Medical Education
https://www.readbyqxmd.com/read/27890403/isoelectric-points-and-points-of-zero-charge-of-metal-hydr-oxides-50years-after-parks-review
#9
REVIEW
Marek Kosmulski
The pH-dependent surface charging of metal (hydr)oxides is reviewed on the occasion of the 50th anniversary of the publication by G.A. Parks: "Isoelectric points of solid oxides, solid hydroxides, and aqueous hydroxo complex systems" in Chemical Reviews. The point of zero charge (PZC) and isoelectric point (IEP) became standard parameters to characterize metal oxides in aqueous dispersions, and they define adsorption (surface excess) of ions, stability against coagulation, rheological properties of dispersions, etc...
December 2016: Advances in Colloid and Interface Science
https://www.readbyqxmd.com/read/27814796/access-to-opportunities-for-bilingualism-for-individuals-with-developmental-disabilities-key-informant-interviews
#10
Julia Scherba de Valenzuela, Elizabeth Kay-Raining Bird, Karisa Parkington, Pat Mirenda, Kate Cain, Andrea A N MacLeod, Eliane Segers
The purpose of this article is to describe the results of a thematic analysis of 79 semi-structured interviews collected at six research sites in four countries in relation to the inclusion and exclusion of students with developmental disabilities (DD) in and from special education and bilingual opportunities. The participants were individuals with expertise either in special needs and/or language education to support bilingualism (e.g., second language (L2) instruction), who served as key informants about service delivery and/or policy in these areas...
September 2016: Journal of Communication Disorders
https://www.readbyqxmd.com/read/27797169/improving-loading-amount-and-performance-of-quantum-dot-sensitized-solar-cells-through-metal-salt-solutions-treatment-on-photoanode
#11
Wenran Wang, Jun Du, Zhenwei Ren, Wenxiang Peng, Zhenxiao Pan, Xinhua Zhong
Increasing QD loading amount on photoanode and suppressing charge recombination are prerequisite for high-efficiency quantum dot-sensitized solar cells (QDSCs). Herein, a facile technique for enhancing the loading amount of QDs on photoanode and therefore improving the photovoltaic performance of the resultant cell devices is developed by pipetting metal salt aqueous solutions on TiO2 film electrode and then evaporating at elevated temperature. The effect of different metal salt solutions was investigated, and experimental results indicated that the isoelectric point (IEP) of metal ions influenced the loading amount of QDs and consequently the photovoltaic performance of the resultant cell devices...
November 16, 2016: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/27765015/recent-horizontal-transfer-functional-adaptation-and-dissemination-of-a-bacterial-group-ii-intron
#12
Félix LaRoche-Johnston, Caroline Monat, Benoit Cousineau
BACKGROUND: Group II introns are catalytically active RNA and mobile retroelements present in certain eukaryotic organelles, bacteria and archaea. These ribozymes self-splice from the pre-mRNA of interrupted genes and reinsert within target DNA sequences by retrohoming and retrotransposition. Evolutionary hypotheses place these retromobile elements at the origin of over half the human genome. Nevertheless, the evolution and dissemination of group II introns was found to be quite difficult to infer...
October 20, 2016: BMC Evolutionary Biology
https://www.readbyqxmd.com/read/27730127/functionality-of-in-vitro-reconstituted-group-ii-intron-rmint1-derived-ribonucleoprotein-particles
#13
Maria D Molina-Sánchez, Fernando M García-Rodríguez, Nicolás Toro
The functional unit of mobile group II introns is a ribonucleoprotein particle (RNP) consisting of the intron-encoded protein (IEP) and the excised intron RNA. The IEP has reverse transcriptase activity but also promotes RNA splicing, and the RNA-protein complex triggers site-specific DNA insertion by reverse splicing, in a process called retrohoming. In vitro reconstituted ribonucleoprotein complexes from the Lactococcus lactis group II intron Ll.LtrB, which produce a double strand break, have recently been studied as a means of developing group II intron-based gene targeting methods for higher organisms...
2016: Frontiers in Molecular Biosciences
https://www.readbyqxmd.com/read/27726484/forks-in-the-tracks-group-ii-introns-spliceosomes-telomeres-and-beyond
#14
Rajendra Kumar Agrawal, Hong-Wei Wang, Marlene Belfort
Group II introns are large catalytic RNAs that form a ribonucleoprotein (RNP) complex by binding to an intron-encoded protein (IEP). The IEP, which facilitates both RNA splicing and intron mobility, has multiple activities including reverse transcriptase. Recent structures of a group II intron RNP complex and of IEPs from diverse bacteria fuel arguments that group II introns are ancestrally related to eukaryotic spliceosomes as well as to telomerase and viruses. Furthermore, recent structural studies of various functional states of the spliceosome allow us to draw parallels between the group II intron RNP and the spliceosome...
December 2016: RNA Biology
https://www.readbyqxmd.com/read/27703021/aspergillus-antibody-detection-diagnostic-strategy-and-technical-considerations-from-the-soci%C3%A3-t%C3%A3-fran%C3%A3-aise-de-mycologie-m%C3%A3-dicale-french-society-for-medical-mycology-expert-committee
#15
F Persat, C Hennequin, J P Gangneux
Until now, there has been no consensus on the best method for the detection of anti-Aspergillus antibodies, a key diagnostic tool for chronic aspergilloses. To better appreciate the usage of and confidence in these techniques, the Société Française de Mycologie Médicale (French Society for Medical Mycology; SFMM) performed a two-step survey of French experts. First, we administered an initial survey to French labs performing Aspergillus serology to depict usage of the different techniques available for Aspergillus serology...
October 4, 2016: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/27696699/enhancement-of-wound-closure-by-modifying-dual-release-patterns-of-stromal-derived-cell-factor-1-and-a-macrophage-recruitment-agent-from-gelatin-hydrogels
#16
Yang-Hee Kim, Yasuhiko Tabata
The objective of the present study is to evaluate the effects of the release patterns of stromal derived factor (SDF)-1 and sphingosine-1 phosphate agonist (SEW2871), used as MSC and macrophage recruitment agents, on the wound closure of diabetic mouse skin defects. To achieve different release patterns, hydrogels were prepared using two types of gelatin with isoelectric points (IEP) of 5 and 9, into which SDF-1 and SEW2871 were then incorporated in various combinations. When the hydrogels incorporating SDF-1 and SEW2871 were applied into wound defects of diabetic mice, the number of MSCs and macrophages recruited to the defects and the levels of pro- and anti- inflammatory cytokines were found to be dependent on the release profiles of SDF-1 and SEW2871...
October 3, 2016: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/27690244/surface-chemistry-from-wettability-and-charge-for-the-control-of-mesenchymal-stem-cell-fate-through-self-assembled-monolayers
#17
Lijing Hao, Xiaoling Fu, Tianjie Li, Naru Zhao, Xuetao Shi, Fuzhai Cui, Chang Du, Yingjun Wang
Self-assembled monolayers (SAMs) of alkanethiols on gold are highly controllable model substrates and have been employed to mimic the extracellular matrix for cell-related studies. This study aims to systematically explore how surface chemistry influences the adhesion, morphology, proliferation and osteogenic differentiation of mouse mesenchymal stem cells (mMSCs) using various functional groups (-OEG, -CH3, -PO3H2, -OH, -NH2 and -COOH). Surface analysis demonstrated that these functional groups produced a wide range of wettability and charge: -OEG (hydrophilic and moderate iso-electric point (IEP)), -CH3 (strongly hydrophobic and low IEP), -PO3H2 (moderate wettability and low IEP), -OH (hydrophilic and moderate IEP), -NH2 (moderate wettability and high IEP) and -COOH (hydrophilic and low IEP)...
December 1, 2016: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/27642209/school-based-screening-to-identify-children-at-risk-for-attention-deficit-hyperactivity-disorder-barriers-and-implications
#18
Tammy D Barry, Raymond A Sturner, Karen Seymour, Barbara H Howard, Lucy McGoron, Paul Bergmann, Ronald Kent, Casey Sullivan, Theodore S Tomeny, Jessica S Pierce, Kristen L Coin, James K Goodlad, Nichole Werle
This report describes a school-based screening project to improve early identification of children at risk for attention-deficit/hyperactivity disorder (ADHD) and communicate these concerns to parents, recommending that they contact their child's primary care provider (PCP). Of 17,440 eligible children in first through fifth grades in five school districts, 47.0% of parents provided required written consent, and teachers completed 70.4% of the online screeners (using the Vanderbilt AD/HD Diagnostic Teacher Rating Scale)...
2016: Children's Health Care: Journal of the Association for the Care of Children's Health
https://www.readbyqxmd.com/read/27612023/effect-of-smile-index-and-incisal-edge-position-on-perception-of-attractiveness-in-different-age-groups
#19
J-C Chou, A Nelson, D Katwal, E N Elathamna, M T Durski
Changes in occlusal vertical dimension (OVD) and age have been found to affect Smile Index (SI, width/height of smile). Limited information is available regarding the aesthetic effects of these changes. The objective of this study was to evaluate the attractiveness of digitally manipulated smile images with differences in SI and incisal edge position (IEP) judged by respondents in different age groups. A total of 12 smile images were generated with varying SI (3·5, 5·3, 7·2, 9·0) and IEP (High, Medium, Low)...
November 2016: Journal of Oral Rehabilitation
https://www.readbyqxmd.com/read/27588750/host-factors-influencing-the-retrohoming-pathway-of-group-ii-intron-rmint1-which-has-an-intron-encoded-protein-naturally-devoid-of-endonuclease-activity
#20
Rafael Nisa-Martínez, María Dolores Molina-Sánchez, Nicolás Toro
Bacterial group II introns are self-splicing catalytic RNAs and mobile retroelements that have an open reading frame encoding an intron-encoded protein (IEP) with reverse transcriptase (RT) and RNA splicing or maturase activity. Some IEPs carry a DNA endonuclease (En) domain, which is required to cleave the bottom strand downstream from the intron-insertion site for target DNA-primed reverse transcription (TPRT) of the inserted intron RNA. Host factors complete the insertion of the intron. By contrast, the major retrohoming pathway of introns with IEPs naturally lacking endonuclease activity, like the Sinorhizobium meliloti intron RmInt1, is thought to involve insertion of the intron RNA into the template for lagging strand DNA synthesis ahead of the replication fork, with possible use of the nascent strand to prime reverse transcription of the intron RNA...
2016: PloS One
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