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Lrrc8a

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https://www.readbyqxmd.com/read/29773801/lrrc8-vrac-anion-channels-enhance-%C3%AE-cell-glucose-sensing-and-insulin-secretion
#1
Till Stuhlmann, Rosa Planells-Cases, Thomas J Jentsch
Glucose homeostasis depends critically on insulin that is secreted by pancreatic β-cells. Serum glucose, which is directly sensed by β-cells, stimulates depolarization- and Ca2+ -dependent exocytosis of insulin granules. Here we show that pancreatic islets prominently express LRRC8A and LRRC8D, subunits of volume-regulated VRAC anion channels. Hypotonicity- or glucose-induced β-cell swelling elicits canonical LRRC8A-dependent VRAC currents that depolarize β-cells to an extent that causes electrical excitation...
May 17, 2018: Nature Communications
https://www.readbyqxmd.com/read/29769723/structure-of-a-volume-regulated-anion-channel-of-the-lrrc8-family
#2
Dawid Deneka, Marta Sawicka, Andy K M Lam, Cristina Paulino, Raimund Dutzler
Volume-regulated anion channels are activated in response to hypotonic stress. These channels are composed of closely related paralogues of the leucine-rich repeat-containing protein 8 (LRRC8) family that co-assemble to form hexameric complexes. Here, using cryo-electron microscopy and X-ray crystallography, we determine the structure of a homomeric channel of the obligatory subunit LRRC8A. This protein conducts ions and has properties in common with endogenous heteromeric channels. Its modular structure consists of a transmembrane pore domain followed by a cytoplasmic leucine-rich repeat domain...
May 16, 2018: Nature
https://www.readbyqxmd.com/read/29498698/expression-of-lrrc8-vrac-currents-in-xenopus-oocytes-advantages-and-caveats
#3
REVIEW
Héctor Gaitán-Peñas, Michael Pusch, Raúl Estévez
Volume-regulated anion channels (VRACs) play a role in controlling cell volume by opening upon cell swelling. Apart from controlling cell volume, their function is important in many other physiological processes, such as transport of metabolites or drugs, and extracellular signal transduction. VRACs are formed by heteromers of the pannexin homologous protein LRRC8A (also named Swell1) with other LRRC8 members (B, C, D, and E). LRRC8 proteins are difficult to study, since they are expressed in all cells of our body, and the channel stoichiometry can be changed by overexpression, resulting in non-functional heteromers...
March 2, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28972132/leucine-rich-repeat-containing-8b-protein-is-associated-with-the-endoplasmic-reticulum-ca-2-leak-in-hek293-cells
#4
Arijita Ghosh, Nitin Khandelwal, Arvind Kumar, Amal Kanti Bera
Leucine-rich repeat-containing 8 (LRRC8) proteins have been proposed to evolutionarily originate from the combination of the channel protein pannexin, and a leucine-rich repeat (LRR) domain. Five paralogs of LRRC8, namely LRRC8A, LRRC8B, LRRC8C, LRRC8D and LRRC8E have been reported. LRRC8A has been shown to be instrumental in cell swelling. Here, we identify LRRC8B as a key player in the cellular Ca2+ signaling network. Overexpression of human LRRC8B in HEK293 cells reduced the Ca2+ level in the endoplasmic reticulum (ER)...
November 15, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28841766/subunit-dependent-oxidative-stress-sensitivity-of-lrrc8-volume-regulated-anion-channels
#5
Antonella Gradogna, Paola Gavazzo, Anna Boccaccio, Michael Pusch
KEY POINTS: Swelling-activated anion currents are modulated by oxidative conditions, but it is unknown if oxidation acts directly on the LRRC8 channel-forming proteins or on regulatory factors. We found that LRRC8A-LRRC8E heteromeric channels are dramatically activated by oxidation of intracellular cysteines, whereas LRRC8A-LRRC8C and LRRC8A-LRRC8D heteromers are inhibited by oxidation. Volume-regulated anion currents in Jurkat T lymphocytes were inhibited by oxidation, in agreement with a low expression of the LRRC8E subunit in these cells...
November 1, 2017: Journal of Physiology
https://www.readbyqxmd.com/read/28833202/molecular-composition-and-heterogeneity-of-the-lrrc8-containing-swelling-activated-osmolyte-channels-in-primary-rat-astrocytes
#6
Alexandra L Schober, Corinne S Wilson, Alexander A Mongin
KEY POINTS: The volume-regulated anion channel (VRAC) is a swelling-activated chloride channel that is permeable to inorganic anions and a variety of small organic molecules. VRAC is formed via heteromerization of LRRC8 proteins, among which LRRC8A is essential, while LRRC8B/C/D/E serve as exchangeable complementary partners. We used an RNAi approach and radiotracer assays to explore which LRRC8 isoforms contribute to swelling-activated release of diverse organic osmolytes in rat astrocytes...
November 15, 2017: Journal of Physiology
https://www.readbyqxmd.com/read/28553230/the-lrrc8a-mediated-swell-activated-chloride-conductance-is-dispensable-for-vacuolar-homeostasis-in-neutrophils
#7
Philippe Behe, Juliet R Foote, Adam P Levine, Craig D Platt, Janet Chou, Fernando Benavides, Raif S Geha, Anthony W Segal
The dialysis of human and mouse neutrophils in patch clamp experiments in the conventional whole-cell mode induces the emergence of a chloride (Cl- ) current that appeared to be primarily regulated by cytoplasmic ionic strength. The characteristics of this current resembled that of the classical, and ubiquitous volume-sensitive outwardly rectifying Cl- current: strong outward rectification, selectivity sequence of the Eisenman1 type, insensitivity to external pH and strong inhibition by tamoxifen, DCPIB and WW781...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28436964/swell1-is-a-regulator-of-adipocyte-size-insulin-signalling-and-glucose-homeostasis
#8
Yanhui Zhang, Litao Xie, Susheel K Gunasekar, Dan Tong, Anil Mishra, William J Gibson, Chuansong Wang, Trevor Fidler, Brodie Marthaler, Aloysius Klingelhutz, E Dale Abel, Isaac Samuel, Jessica K Smith, Lei Cao, Rajan Sah
Adipocytes undergo considerable volumetric expansion in the setting of obesity. It has been proposed that such marked increases in adipocyte size may be sensed via adipocyte-autonomous mechanisms to mediate size-dependent intracellular signalling. Here, we show that SWELL1 (LRRC8a), a member of the Leucine-Rich Repeat Containing protein family, is an essential component of a volume-sensitive ion channel (VRAC) in adipocytes. We find that SWELL1-mediated VRAC is augmented in hypertrophic murine and human adipocytes in the setting of obesity...
May 2017: Nature Cell Biology
https://www.readbyqxmd.com/read/28289711/the-volume-regulated-anion-channel-lrrc8-in-nodose-neurons-is-sensitive-to-acidic-ph
#9
Runping Wang, Yongjun Lu, Susheel Gunasekar, Yanhui Zhang, Christopher J Benson, Mark W Chapleau, Rajan Sah, François M Abboud
The leucine rich repeat containing protein 8A (LRRC8A), or SWELL1, is an essential component of the volume-regulated anion channel (VRAC) that is activated by cell swelling and ionic strength. We report here for the first time to our knowledge its expression in a primary cell culture of nodose ganglia neurons and its localization in the soma, neurites, and neuronal membrane. We show that this neuronal VRAC/SWELL1 senses low external pH (pHo ) in addition to hypoosmolarity. A robust sustained chloride current is seen in 77% of isolated nodose neurons following brief exposures to extracellular acid pH...
March 9, 2017: JCI Insight
https://www.readbyqxmd.com/read/28193731/selective-transport-of-neurotransmitters-and-modulators-by-distinct-volume-regulated-lrrc8-anion-channels
#10
Darius Lutter, Florian Ullrich, Jennifer C Lueck, Stefan Kempa, Thomas J Jentsch
In response to swelling, mammalian cells release chloride and organic osmolytes through volume-regulated anion channels (VRACs). VRACs are heteromers of LRRC8A and other LRRC8 isoforms (LRRC8B to LRRC8E), which are co-expressed in HEK293 and most other cells. The spectrum of VRAC substrates and its dependence on particular LRRC8 isoforms remains largely unknown. We show that, besides the osmolytes taurine and myo-inositol, LRRC8 channels transport the neurotransmitters glutamate, aspartate and γ-aminobutyric acid (GABA) and the co-activator D-serine...
March 15, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28192143/leucine-rich-repeat-containing-8a-lrrc8a-dependent-volume-regulated-anion-channel-activity-is-dispensable-for-t-cell-development-and-function
#11
Craig D Platt, Janet Chou, Patrick Houlihan, Yousef R Badran, Lalit Kumar, Wayne Bainter, P Luigi Poliani, Carlos J Perez, Sharon Y R Dent, David E Clapham, Fernando Benavides, Raif S Geha
BACKGROUND: Leucine-rich repeat containing 8A (LRRC8A) is an ubiquitously expressed transmembrane protein with 17 leucine-rich repeats (LRRs) at its C-terminal end and is an essential component of the volume-regulated anion channel (VRAC), which controls cellular volume. A heterozygous mutation in LRRC8A that truncates the 2 terminal LRRs was reported in a patient with agammaglobulinemia and absent B cells and was demonstrated to exert a dominant negative effect on T- and B-cell development in mice...
December 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28121479/cisplatin-activates-volume-sensitive-lrrc8-channel-mediated-currents-in-xenopus-oocytes
#12
Antonella Gradogna, Héctor Gaitán-Peñas, Anna Boccaccio, Raúl Estévez, Michael Pusch
LRRC8 proteins have been shown to underlie the ubiquitous volume regulated anion channel (VRAC). VRAC channels are composed of the LRRC8A subunit and at least one among the LRRC8B-E subunits. In addition to their role in volume regulation, LRRC8 proteins have been implicated in the uptake of chemotherapeutic agents. We had found that LRRC8 channels can be conveniently expressed in Xenopus oocytes, a system without endogenous VRAC activity. The fusion with fluorescent proteins yielded constitutive activity for A/C, A/D and A/E heteromers...
May 4, 2017: Channels
https://www.readbyqxmd.com/read/27764579/specific-and-essential-but-not-sufficient-roles-of-lrrc8a-in-the-activity-of-volume-sensitive-outwardly-rectifying-anion-channel-vsor
#13
Toshiaki Okada, Md Rafiqul Islam, Nargiza A Tsiferova, Yasunobu Okada, Ravshan Z Sabirov
The broadly expressed volume-sensitive outwardly rectifying anion channel (VSOR, also called VRAC) plays essential roles in cell survival and death. Recent findings have suggested that LRRC8A is a core component of VSOR in human cells. In the present study, VSOR currents were found to be largely reduced by siRNA against LRRC8A in mouse C127 cells as well. In contrast, LRRC8A knockdown never affected activities of 4 other types of anion channel activated by acid, Ca(2+), patch excision or cAMP. While cisplatin-resistant KCP-4 cells poorly expressed endogenous VSOR activity, molecular expression levels of LRRC8A, LRRC8D and LRRC8E were indistinguishable between VSOR-deficient KCP-4 cells and the parental VSOR-rich KB cells...
March 4, 2017: Channels
https://www.readbyqxmd.com/read/27705766/investigation-of-lrrc8-mediated-volume-regulated-anion-currents-in-xenopus-oocytes
#14
Héctor Gaitán-Peñas, Antonella Gradogna, Lara Laparra-Cuervo, Carles Solsona, Victor Fernández-Dueñas, Alejandro Barrallo-Gimeno, Francisco Ciruela, Melike Lakadamyali, Michael Pusch, Raúl Estévez
Volume-regulated anion channels (VRACs) play an important role in controlling cell volume by opening upon cell swelling. Recent work has shown that heteromers of LRRC8A with other LRRC8 members (B, C, D, and E) form the VRAC. Here, we used Xenopus oocytes as a simple system to study LRRC8 proteins. We discovered that adding fluorescent proteins to the C-terminus resulted in constitutive anion channel activity. Using these constructs, we reproduced previous findings indicating that LRRC8 heteromers mediate anion and osmolyte flux with subunit-dependent kinetics and selectivity...
October 4, 2016: Biophysical Journal
https://www.readbyqxmd.com/read/27688432/leucine-rich-repeat-containing-protein-lrrc8a-is-essential-for-swelling-activated-cl-currents-and-embryonic-development-in-zebrafish
#15
Toshiki Yamada, Robert Wondergem, Rebecca Morrison, Viravuth P Yin, Kevin Strange
A volume-regulated anion channel (VRAC) has been electrophysiologically characterized in innumerable mammalian cell types. VRAC is activated by cell swelling and mediates the volume regulatory efflux of Cl(-) and small organic solutes from cells. Two groups recently identified the mammalian leucine-rich repeat containing protein LRRC8A as an essential VRAC component. LRRC8A must be coexpressed with at least one of the other four members of this gene family, LRRC8B-E, to reconstitute VRAC activity in LRRC8(-/-) cells...
October 2016: Physiological Reports
https://www.readbyqxmd.com/read/27579940/distinct-contributions-of-lrrc8a-and-its-paralogs-to-the-vsor-anion-channel-from-those-of-the-asor-anion-channel
#16
Kaori Sato-Numata, Tomohiro Numata, Ryuji Inoue, Ravshan Z Sabirov, Yasunobu Okada
Volume- and acid-sensitive outwardly rectifying anion channels (VSOR and ASOR) activated by swelling and acidification exhibit voltage-dependent inactivation and activation time courses, respectively. Recently, LRRC8A and some paralogs were shown to be essentially involved in the activity and inactivation kinetics of VSOR currents in human colonic HCT116 cells. In human cervix HeLa cells, here, inactivation of VSOR currents was found to become accelerated by RNA silencing only of LRRC8A but never decelerated by that of any LRRC8 isoform...
March 4, 2017: Channels
https://www.readbyqxmd.com/read/27514381/relationship-between-tmem16a-anoctamin-1-and-lrrc8a
#17
Roberta Benedetto, Lalida Sirianant, Ines Pankonien, Podchanart Wanitchakool, Jiraporn Ousingsawat, Ines Cabrita, Rainer Schreiber, Margarida Amaral, Karl Kunzelmann
TMEM16A/anoctamin 1/ANO1 and VRAC/LRRC8 are independent chloride channels activated either by increase in intracellular Ca(2+) or cell swelling, respectively. In previous studies, we observed overlapping properties for both types of channels. (i) TMEM16A/ANO1 and LRRC8 are inhibited by identical compounds, (ii) the volume-regulated anion channel VRAC requires compartmentalized Ca(2+) increase to be fully activated, (iii) anoctamins are activated by cell swelling, (iv) both channels have a role for apoptotic cell death, (v) both channels are possibly located in lipid rafts/caveolae like structures, and (vi) VRAC and anoctamin 1 currents are not additive when each are fully activated...
October 2016: Pflügers Archiv: European Journal of Physiology
https://www.readbyqxmd.com/read/27358231/channels-and-volume-changes-in-the-life-and-death-of-the-cell
#18
REVIEW
Herminia Pasantes-Morales
Volume changes deviating from original cell volume represent a major challenge for cellular homeostasis. Cell volume may be altered either by variations in the external osmolarity or by disturbances in the transmembrane ion gradients that generate an osmotic imbalance. Cells respond to anisotonicity-induced volume changes by active regulatory mechanisms that modify the intracellular/extracellular concentrations of K(+), Cl(-), Na(+), and organic osmolytes in the direction necessary to reestablish the osmotic equilibrium...
September 2016: Molecular Pharmacology
https://www.readbyqxmd.com/read/27325695/inactivation-and-anion-selectivity-of-volume-regulated-anion-channels-vracs-depend-on-c-terminal-residues-of-the-first-extracellular-loop
#19
Florian Ullrich, S Momsen Reincke, Felizia K Voss, Tobias Stauber, Thomas J Jentsch
Canonical volume-regulated anion channels (VRACs) are crucial for cell volume regulation and have many other important roles, including tumor drug resistance and release of neurotransmitters. Although VRAC-mediated swelling-activated chloride currents (ICl,vol) have been studied for decades, exploration of the structure-function relationship of VRAC has become possible only after the recent discovery that VRACs are formed by differently composed heteromers of LRRC8 proteins. Inactivation of ICl,vol at positive potentials, a typical hallmark of VRACs, strongly varies between native cell types...
August 12, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27270446/cl-channels-in-apoptosis
#20
Podchanart Wanitchakool, Jiraporn Ousingsawat, Lalida Sirianant, Nanna MacAulay, Rainer Schreiber, Karl Kunzelmann
A remarkable feature of apoptosis is the initial massive cell shrinkage, which requires opening of ion channels to allow release of K(+), Cl(-), and organic osmolytes to drive osmotic water movement and cell shrinkage. This article focuses on the role of the Cl(-) channels LRRC8, TMEM16/anoctamin, and cystic fibrosis transmembrane conductance regulator (CFTR) in cellular apoptosis. LRRC8A-E has been identified as a volume-regulated anion channel expressed in many cell types. It was shown to be required for regulatory and apoptotic volume decrease (RVD, AVD) in cultured cell lines...
October 2016: European Biophysics Journal: EBJ
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