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chemiluminescence immunoassay

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https://www.readbyqxmd.com/read/29782819/analytical-performance-of-an-immunoassay-to-measure-proenkephalin
#1
Leslie J Donato, Jeffrey W Meeusen, John C Lieske, Deborah Bergmann, Andrea Sparwaßer, Allan S Jaffe
BACKGROUND: Endogenous opioids, enkephalins, are known to increase with acute kidney injury. Since the mature pentapeptides are unstable, we evaluated the performance of an assay that measures proenkephalin 119-159 (PENK), a stable peptide formed concomitantly with mature enkephalins. METHODS: PENK assay performance was evaluated on two microtiterplate/chemiluminescence sandwich immunoassay formats that required 18 or 1 h incubation times. PENK concentration was measured in plasma from healthy individuals to establish a reference interval and in patients with varied levels of kidney function and comorbidities to assess the association with measured glomerular filtration rate (mGFR) using iothalamate clearance...
May 18, 2018: Clinical Biochemistry
https://www.readbyqxmd.com/read/29782266/-the-association-of-gene-polymorphisms-of-matrix-metalloproteinases-9-12-and-20-and-collagen-type-i-degradation-products-with-the-remodeling-of-the-left-ventricle-in-patients-with-acute-myocardial-infarction
#2
N A Sokolova, M S Danshova, A V Govorin, D N Zaytsev, Y N Smolyakov
OBJECTIVE: to study associations of polymorphism of the genes MMP9 rs17576, MMP12 rs2276109, MMP20 rs2245803, COL1A1 rs1800012, COL1A1 rs1107946 with remodeling of the left ventricle in patients with acute myocardial infarction. MATERIALS AND METHODS: We examined 84 patients with myocardial infarction (MI). Type of early postinfarction left ventricular (LV) remodeling was determined by echocardiography on the third day of the disease. Concentration of matrix metalloproteinases (MMP) was measured by enzyme immunoassay using commercial kits; content of C-terminal telopeptide of collagen type I degradation products (ICTP) was measured by chemiluminescent immunoassay...
March 2018: Kardiologiia
https://www.readbyqxmd.com/read/29769276/analytical-comparison-of-the-architect-syphilis-tp-and-liaison-treponema-assay-automated-chemiluminescent-immunoassays-and-their-performance-in-a-reverse-syphilis-screening-algorithm
#3
Alan M Sanfilippo, Kristie Freeman, John L Schmitz
Syphilis incidence has increased in the United States since 2000 (1, 2) and remains a global public health concern (3, 4).….
May 16, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29754604/electrochemiluminescence-immunoassay-based-on-site-specific-labeling-using-a-histidine-binding-iridium-iii-solvento-complex
#4
Ming Zhou, Ying Xia
Histidine bound bis-cyclometalated iridium (III) complexes were found to be luminescent in previous studies. This work demonstrates that the luminophores could be excited electrochemically and the electrogenerated chemiluminescence was observed in both homogeneous solutions and on the surface of magnetic beads. The findings laid a foundation for novel ECL immunoassay methodology featuring site-specific labeling of antibodies and on-site formation of signal-generating units. Taking use of the findings and the unique advantages of the site-specific labeling approach, a sandwich immunoassay for a clinically important biomarker was conceptually proved...
September 6, 2018: Analytica Chimica Acta
https://www.readbyqxmd.com/read/29751358/vitamin-d-was-not-associated-with-survival-or-cerebrospinal-fluid-cathelicidin-levels-in-children-with-bacterial-meningitis
#5
Okko Savonius, Tuula Pelkonen, Irmeli Roine, Heli Viljakainen, Sture Andersson, Josefina Fernandez, Heikki Peltola, Otto Helve
AIM: Vitamin D deficiency impairs the immunological system and has been associated with worse outcomes of infectious diseases, but its role in bacterial meningitis remains unknown. We investigated whether serum 25-hydroxyvitamin D concentrations related to disease outcomes and to cerebrospinal fluid cathelicidin concentrations in childhood bacterial meningitis. METHODS: All consecutively enrolled patients in a clinical trial on childhood bacterial meningitis in Latin America in 1996-2003 were considered and 142 children, with a median age of seven months who had a confirmed bacterial aetiology and frozen serum available for further analyses, were included in the present study...
May 11, 2018: Acta Paediatrica
https://www.readbyqxmd.com/read/29743307/evaluation-of-modified-two-tiered-testing-algorithms-for-lyme-disease-laboratory-diagnosis-using-well-characterized-serum-samples
#6
Adoracion Pegalajar-Jurado, Martin E Schriefer, Ryan J Welch, Marc R Couturier, Tiffany MacKenzie, Rebecca J Clark, Laura V Ashton, Mark J Delorey, Claudia R Molins
Standard two-tiered testing (STTT) is the recommended algorithm for laboratory diagnosis of Lyme disease (LD). Several limitations are associated with STTT that include low sensitivity in the early stages of disease, as well as technical complexity and subjectivity associated with second-tier immunoblots; therefore, modified two-tiered testing (MTTT) algorithms that utilize two sequential first-tier tests and eliminate immunoblots have been evaluated. Recently, a novel MTTT that uses a VlsE chemiluminescence immunoassay followed by a C6 enzyme immunoassay has been proposed...
May 9, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29741399/enzalutamide-and-analytical-interferences-in-digoxin-assays
#7
Marie Deguigne, Marion Brunet, Chadi Abbara, Alain Turcant, Gaël Le Roux, Bénédicte Lelièvre
OBJECTIVE: We report two cases of elevated digoxin plasma levels in patients receiving enzalutamide. Cases reported: The first patient, an 84-year-old male treated with enzalutamide, was hospitalized due to deterioration in his general state. Atrial fibrillation was discovered and treatment with digoxin was initiated. Supratherapeutic digoxin concentrations (4 µg/L and 3.5 µg/L 3 days later) led to treatment being stopped despite the lack of clinical or biological signs of overdose...
May 9, 2018: Clinical Toxicology
https://www.readbyqxmd.com/read/29739378/higher-serum-25-hydroxyvitamin-d-concentrations-are-associated-with-active-pulmonary-tuberculosis-in-hospitalised-hiv-infected-patients-in-a-low-income-tropical-setting-a-cross-sectional-study
#8
Cuthbert Musarurwa, Lynn Sodai Zijenah, Doreen Zvipo Mhandire, Tsitsi Bandason, Kudakwashe Mhandire, Maria Mary Chipiti, Marshall Wesley Munjoma, Witmore Bayayi Mujaji
BACKGROUND: The inherent risk of developing tuberculosis (TB) in HIV- infected individuals is further enhanced by hypovitaminosis D. Interventions that offset HIV-associated immune deterioration potentially arrest disease progression and incidence of opportunistic infections including TB. Despite conflicting reports on association between vitamin D deficiency (VDD) and risk of TB, vitamin D (VD) supplementation remains a promising intervention. METHODS: We conducted a comparative cross-sectional study on 145 HIV+ /pulmonary TB+ (PTB) and 139 HIV+ /PTB- hospitalised patients to investigate association of vitamin D status and risk of PTB...
May 8, 2018: BMC Pulmonary Medicine
https://www.readbyqxmd.com/read/29739124/verification-of-bioanalytical-method-for-quantification-of-exogenous-insulin-insulin-aspart-by-the-analyser-advia-centaur%C3%A2-xp
#9
Rossen Mihailov, Dilyana Stoeva, Blagovesta Pencheva, Eugeni Pentchev
BACKGROUND: In a number of cases the monitoring of patients with type I diabetes mellitus requires measurement of the exogenous insulin levels. For the purpose of a clinical investigation of the efficacy of a medical device for application of exogenous insulin aspart, a verification of the method for measurement of this synthetic analogue of the hormone was needed. The information in the available medical literature for the measurement of the different exogenous insulin analogs is insufficient...
March 1, 2018: Clinical Laboratory
https://www.readbyqxmd.com/read/29738696/serum-human-epididymis-secretory-protein-4-as-a-potential-biomarker-of-renal-fibrosis-in-kidney-transplantation-recipients
#10
Jinmei Luo, Fen Wang, Jianxin Wan, Zhuangjian Ye, Chumei Huang, Yuesu Cai, Min Liu, Ben-Quan Wu, Laisheng Li
BACKGROUND: Renal fibrosis remains an important cause of kidney allograft failure. The objective of this study was to evaluate the performance of serum human epididymis secretory protein 4 (HE4) as a biomarker for renal fibrosis in kidney transplant recipients. METHODS: A total of 103 kidney transplantation patients were enrolled in this study, and serum HE4 concentrations were detected using the chemiluminescent microparticle immunoassay. Renal biopsy was carried out, and histological findings were assessed by immunohistochemistry...
May 5, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/29732941/measurement-of-cerebral-biomarkers-proving-traumatic-brain-injuries-in-post-mortem-body-fluids
#11
Benjamin Ondruschka, Monique Sieber, Holger Kirsten, Heike Franke, Jan Dressler
Until now, it is impossible to identify a fatal traumatic brain injury (TBI) before post-mortem radiological investigations or an autopsy take place. It would be preferable to have an additional diagnostic tool like post-mortem biochemistry to get greater insight into the pathological pathways and survival times after sustaining TBI. Cerebrospinal fluid (CSF) and serum samples of 84 autopsy cases were collected from forensic autopsies with post-mortem intervals (PMI) of up to 148 h. The cases were categorized into a fatal TBI case group (n=42) and non-TBI controls (n=42)...
May 5, 2018: Journal of Neurotrauma
https://www.readbyqxmd.com/read/29729233/simultaneous-quantitation-of-carbohydrate-antigen-125-and-carcinoembryonic-antigen-in-human-serum-via-time-resolved-fluoroimmunoassay
#12
Zhen Huang, Xiang-Ming Zhai, Hao Wang, Qiao-Ting Deng, Kun Li, Bi-Sen Liu, Gang Wang, Tian-Cai Liu
In clinical diagnosis of cancer, immunology assay with single tumor marker often lead to a false and missed inspection. A quantitative method with a high degree of accuracy, sensitivity, and effectiveness is required for its diagnosis. We developed a dual-label time-resolved fluoroimmunoassay (TRFIA) to simultaneously detect carbohydrate antigen 125 (CA125) and carcinoembryonic antigen (CEA) in human serum to aid the diagnosis and prognosis of gastric cancer. The method was based on a microplate sandwich immunoassay using europium-labeled anti-CA125 antibodies and samarium-labeled anti-CEA antibodies as fluorescent reporters...
May 2, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/29727158/assembly-of-multifunctionalized-gold-nanoparticles-with-chemiluminescent-catalytic-and-immune-activity-for-label-free-immunoassays
#13
Yao Huang, Lingfeng Gao, Hua Cui
In this study, we report a universal label-free immunoassay to detect antigen based on multifunctionalized gold nanoparticles (MF-GNPs), which were obtained by successive assembly of N-aminobutyl-N-ethylisoluminol functionalized gold nanoparticles (ABEI-GNPs) with antibody, bovine serum albumin (BSA) and Co2+ . MF-GNPs exhibited excellent chemiluminescent (CL), catalytic and immune activity. It was demonstrated that the CL signal of MF-GNPs decreased in the presence of antigens via antigen-antibody specific binding using human immunoglobulin G (hIgG) and corresponding antibody goat-anti-human IgG (anti-hIgG) as a model system, due to that immunoreaction led to the aggregation of GNPs...
May 4, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29725479/detection-of-urinary-survivin-using-a-magnetic-particles-based-chemiluminescence-immunoassay-for-the-preliminary-diagnosis-of-bladder-cancer-and-renal-cell-carcinoma-combined-with-laptm4b
#14
Yang Yang, Jianjun Xu, Qingyun Zhang
The aim of the present study was to establish a simple step magnetic particles (MPs) based chemiluminescence enzyme immunoassay (CLEIA) for the detection of urinary survivin, and to investigate the diagnostic value of urinary survivin and lysosome-associated protein transmembrane-4β (LAPTM4B) in bladder cancer (BC) and renal cell carcinoma (RCC). The MPs-based CLEIA was developed on the basis of a double antibodies sandwich immunoreaction and luminol-H2 O2 chemiluminescence system. The parameters of the method were optimized and evaluated...
May 2018: Oncology Letters
https://www.readbyqxmd.com/read/29713602/chemiluminescence-based-immunoassay-for-the-detection-of-heroin-and-its-metabolites
#15
Smritee Singh, Priya Mishra, Ivneet Banga, Avanish S Parmar, Prem Prakash Tripathi, Sonu Gandhi
Introduction: Continuous use of opiates causes drug-related illnesses, which poses an alarming situation to develop sensitive detection platform. In this study, a highly sensitive and reliable chemiluminescence immunoassay (CI) has been developed for the detection of heroin and its major metabolites in spiked urine samples. Methods: To develop robust immunoassay, monoacetyl morphine-bovine serum albumin (MAM-BSA) conjugate was synthesized and characterized thoroughly by physicochemical techniques. The anti-MAM antibodies were developed, labeled with horseradish peroxidase (HRP) and immunoassay was developed to detect the presence of target drug in spiked urine samples...
2018: BioImpacts: BI
https://www.readbyqxmd.com/read/29704799/simultaneous-determination-of-cyclosporine-and-tacrolimus-in-human-whole-blood-by-ultra-high-performance-liquid-chromatography-tandem-mass-spectrometry-and-comparison-with-a-chemiluminescence-microparticle-immunoassay
#16
Shenghui Mei, Jiaqing Wang, Di Chen, Leting Zhu, Ming Zhao, Xiaoxin Tian, Xin Hu, Zhigang Zhao
Overestimation of immunoassays for cyclosporine (CsA) and tacrolimus (TAC) analysis in human whole blood is a problem. The liquid chromatography tandem mass spectrometry is recommended as a golden method for CsA and TAC analysis. The aim of the study is to develop and validate an ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) method for simultaneous determination of CsA and TAC in human whole blood and evaluate its agreement with a chemiluminescence microparticle immunoassay (CMIA)...
April 13, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29699957/iodine-deficiency-in-pregnant-women-at-first-trimester-in-ankara
#17
Kazibe Koyuncu, Batuhan Turgay, Feride Söylemez
OBJECTIVE: Iodine deficiency in pregnant woman in Ankara was shown in previous studies. We aimed to conduct a study in a tertiary center to investigate for the need for iodine replacement in our population. MATERIAL AND METHODS: This was a single tertiary center, non-interventional, retrospective, cross-sectional study. Data were retrieved retrospectively from 440 women who had been in first trimester in gestational age. Maternal iodine status, TSH levels, T4 levels were examined...
April 27, 2018: Journal of the Turkish German Gynecological Association
https://www.readbyqxmd.com/read/29692313/the-prevalence-of-hepatitis-c-virus-antibody-in-hiv-negative-persons-in-kenya-2007
#18
Kathleen N Ly, Andrea A Kim, Jan Drobenuic, Maja Kodani, Joel M Montgomery, Barry S Fields, Eyasu H Teshale
The prevalence of hepatitis C virus (HCV) infection in the Kenyan population has not been previously determined. We estimated the Kenyan HCV prevalence in HIV-negative persons aged 15-64 years. This is a retrospective cross-sectional study using data from the 2007 Kenya AIDS Indicator Survey-a nationally representative sample of 15,853 persons aged 15-64 years who completed a health interview and provided a blood specimen. Of the 1,091 randomly selected participants, 50 tested positive for HCV antibody using the automated chemiluminescence immunoassay, corresponding to a weighted HCV antibody positivity rate of 4...
April 23, 2018: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/29684370/analytical-validation-and-investigation-on-reference-intervals-of-aldosterone-and-renin-in-chinese-han-population-by-using-fully-automated-chemiluminescence-immunoassays
#19
Lingyan Deng, Zhigang Xiong, Huijun Li, Xiaomei Lei, Liming Cheng
BACKGROUND: The aldosterone/renin ratio (ARR) is recommended to screen for primary aldosteronism (PA) in hypertension. We estimated fully automated chemiluminescence immunoassays (CLIA) for plasma aldosterone concentrations (PAC) and plasma direct renin concentrations (PRC) and investigated their reference intervals in Chinese Han population. METHODS: PAC and PRC were measured on a fully automated analyzer (LIAISON XL, DiaSorin, Italy). Performance characteristics were estimated according to CLSI proved guidelines...
April 20, 2018: Clinical Biochemistry
https://www.readbyqxmd.com/read/29667557/modification-of-sexual-hormones-in-rheumatoid-arthritis-patients-by-m2000-%C3%AE-d-mannuronic-acid-as-a-novel-nsaid-with-immunosuppressive-property
#20
Mozhgan Jahanbakhshi, Zohreh Babaloo, Seyed Shahabeddin Mortazavi-Jahromi, Maryam Monsef Shokri, Hossein Ahmadi, Abbas Mirshafiey
BACKGROUND: Based on in-vitro, in-vivo and human studies, the β-D-mannuronic acid (M2000) has been introduced as a novel non-steroidal anti-inflammatory drug (NSAID) with immunosuppressive properties. OBJECTIVE: This study aimed to evaluate the efficacy of this drug on serum level of sex hormones (Estradiol, Progesterone, and DHEAS) in rheumatoid arthritis (RA) patients. METHODS: The present research was performed on 10 RA patients who had an inadequate response to conventional treatments (clinical trial identifier: IRCT2014011213739N2)...
April 17, 2018: Endocrine, Metabolic & Immune Disorders Drug Targets
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