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chemiluminescence immunoassay

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https://www.readbyqxmd.com/read/27914221/prevalence-of-trypanosoma-cruzi-hiv-coinfection-in-southern-brazil
#1
Dulce Stauffert, Mariangela Freitas da Silveira, Marília Arndt Mesenburg, Adriane Brod Manta, Alessandra da Silva Dutra, Guilherme Lucas de Oliveira Bicca, Marcos Marreiro Villela
Chagas disease reactivation has been a defining condition for acquired immune deficiency syndrome in Brazil for individuals coinfected with Trypanosoma cruzi and HIV since 2004. Although the first coinfection case was reported in the 1980s, its prevalence has not been firmly established. In order to know coinfection prevalence, a cross-sectional study of 200 HIV patients was performed between January and July 2013 in the city of Pelotas, in southern Rio Grande do Sul, an endemic area for Chagas disease. Ten subjects were found positive for T...
November 30, 2016: Brazilian Journal of Infectious Diseases
https://www.readbyqxmd.com/read/27906594/the-relationship-between-thyroid-function-and-the-prevalence-of-type-2-diabetes-mellitus-in-euthyroid-subjects
#2
Yeqing Gu, Huihui Li, Xue Bao, Qing Zhang, Li Liu, Ge Meng, Hongmei Wu, Huanmin Du, Hongbin Shi, Yang Xia, Qian Su, Liyun Fang, Fei Yu, Huijun Yang, Bin Yu, Shaomei Sun, Xing Wang, Ming Zhou, Qiyu Jia, Qi Guo, Hong Chang, Guolin Wang, Guowei Huang, Kun Song, Kaijun Niu
PURPOSE: The thyroid hormones (TH) are primarily responsible for the regulation of energy balance and metabolism, suggesting that TH levels may contribute to the development of type 2 diabetes mellitus (T2DM). However, few studies have investigated the relationship between TH and T2DM in a general population. The aim of this study is to evaluate whether serum TH levels within the reference range are related to T2DM. METHODS: A cross-sectional study (n=15,296) was performed in Tianjin, China...
December 1, 2016: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/27899969/diagnostic-usefulness-of-the-risk-of-ovarian-malignancy-algorithm-using-the-electrochemiluminescence-immunoassay-for-he4-and-the-chemiluminescence-microparticle-immunoassay-for-ca125
#3
Anita Chudecka-Głaz, Aneta Cymbaluk-Płoska, Katarzyna Luterek-Puszyńska, Janusz Menkiszak
The present study aimed to investigate the usefulness of the Risk of Ovarian Malignancy Algorithm (ROMA) in the preoperative stratification of patients with ovarian tumors using a novel combination of laboratory tests. The study group (n=619) consisted of 354 premenopausal and 265 postmenopausal patients. The levels of carbohydrate antigen 125 (CA125) and human epididymis protein 4 (HE4) were determined, and ROMA calculations were performed for each pre- and postmenopausal patient. HE4 levels were determined using an electrochemiluminescence immunoassay, while CA125 levels were determined by a chemiluminescence microparticle immunoassay...
November 2016: Oncology Letters
https://www.readbyqxmd.com/read/27896669/detection-of-autoantibodies-to-dsf70-ledgfp75-in-mexican-hispanics-using-multiple-complementary-assay-platforms
#4
Mónica Vázquez-Del Mercado, Eduardo Gómez-Bañuelos, Rosa Elena Navarro-Hernández, Oscar Pizano-Martinez, Adan Saldaña-Millán, Efrain Chavarria-Avila, Lorena Gonzalez-Rosas, Lilia Andrade-Ortega, Miguel Angel Saavedra, Olga Lidia Vera-Lastra, Luis Javier Jara, Gabriel Medrano-Ramírez, Claudia Cruz-Reyes, Ignacio García-De la Torre, Marta Escarra-Senmarti, Lisiane Maria Enriconi-Dos Anjos, Anamika Basu, Roger Albesa, Michael Mahler, Carlos A Casiano
PURPOSE: Antinuclear autoantibodies (ANA) targeting the dense fine speckled antigen DFS70, also known as lens epithelium-derived growth factor p75 (LEDGF/p75), are attracting attention due to their low frequency in systemic rheumatic diseases but increased frequency in clinical laboratory referrals and healthy individuals (HI). These ANA specifically recognize the stress protein DFS70/LEDGFp75, implicated in cancer, HIV-AIDS, and inflammation. While their frequency has been investigated in various ethnic populations, there is little information on their frequency among Hispanics/Latinos...
December 2017: Auto- Immunity Highlights
https://www.readbyqxmd.com/read/27895276/rubella-immunity-in-pregnant-native-taiwanese-and-immigrants-from-asian-countries
#5
Yeong-Hwa Zen, Ching-Tang Shih, Wan-Ju Kung, Chien-Hung Lee, Ching-Chiang Lin
Vaccination is considered the most effective method to prevent rubella spread and congenital rubella syndrome (CRS). The aim of the present study was to investigate the rubella immunity among native and immigrant pregnant women in Taiwan. From 2000 to 2014, a total of 16,879 pregnant women who received routine prenatal examinations were recruited in this study. The rubella IgG antibodies were assayed using a microparticle enzyme immunoassay or chemiluminescent microparticle immunoassay. Subjects were categorized by nationality and subcategorized by specific periods of time for comparison...
November 28, 2016: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/27882133/effects-of-forced-swimming-stress-on-thyroid-function-pituitary-thyroid-stimulating-hormone-and-hypothalamus-thyrotropin-releasing-hormone-expression-in-adrenalectomy-wistar-rats
#6
Qiuyan Sun, Aihua Liu, Yanan Ma, Anyi Wang, Xinhong Guo, Weiping Teng, Yaqiu Jiang
In order to study the impact that is imposed on the hypothalamic-pituitary-thyroid (HPT) axis of adrenalectomy male Wistar rats by stress caused by swimming, the blood level of triiodothyronine (T3), thyroxine (T4) and thyroid-stimulating hormone (TSH), the expression of TSHβ mRNA at the pituitary and thyrotropin releasing hormone (TRH) expression at the paraventricular nucleus (PVN) were measured. A total of 50 male Wistar rats of 6-8 weeks of age and with an average weight of 190-210 grams were randomly divided into the following two groups: The surgical (without adrenal glands) and non-surgical (adrenalectomy) group...
November 2016: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/27881117/novel-prokaryotic-expression-of-thioredoxin-fused-insulinoma-associated-protein-tyrosine-phosphatase-2-ia-2-its-characterization-and-immunodiagnostic-application
#7
Luciano Lucas Guerra, Natalia Inés Faccinetti, Aldana Trabucchi, Bruno David Rovitto, Adriana Victoria Sabljic, Edgardo Poskus, Ruben Francisco Iacono, Silvina Noemí Valdez
BACKGROUND: The insulinoma associated protein tyrosine phosphatase 2 (IA-2) is one of the immunodominant autoantigens involved in the autoimmune attack to the beta-cell in Type 1 Diabetes Mellitus. In this work we have developed a complete and original process for the production and recovery of the properly folded intracellular domain of IA-2 fused to thioredoxin (TrxIA-2ic) in Escherichia coli GI698 and GI724 strains. We have also carried out the biochemical and immunochemical characterization of TrxIA-2icand design variants of non-radiometric immunoassays for the efficient detection of IA-2 autoantibodies (IA-2A)...
November 24, 2016: BMC Biotechnology
https://www.readbyqxmd.com/read/27874311/long-term-persistence-in-protection-and-response-to-a-hepatitis-b-vaccine-booster-among-adolescents-immunized-in-infancy-in-the-western-region-of-china
#8
Zhen-Zi Wang, Yu-Hua Gao, Wei Lu, Cun-Duo Jin, Ying Zeng, Ling Yan, Feng Ding, Tong Li, Xue-En Liu, Hui Zhuang
OBJECTIVES: To evaluate the persistence of protection from hepatitis B (HB) vaccination among adolescents immunized with a primary series of HB vaccine as infants, and the immune response to booster doses. METHODS: Healthy adolescents aged 15-17 years vaccinated with HB vaccine only at birth were enrolled. Baseline serum hepatitis B surface antigen (HBsAg), antibody against hepatitis B surface antigen (anti-HBs) and antibody against hepatitis B core antigen (anti-HBc) were detected by Enzyme-Linked Immunosorbent Assay (ELISA) and anti-HBs level was measured using Chemiluminescent Microparticle Immunoassay (CMIA)...
November 22, 2016: Human Vaccines & Immunotherapeutics
https://www.readbyqxmd.com/read/27873208/validation-procedure-for-multiplex-antibiotic-immunoassays-using-flow-based-chemiluminescence-microarrays
#9
Verena Katharina Meyer, Daniela Meloni, Fabio Olivo, Erwin Märtlbauer, Richard Dietrich, Reinhard Niessner, Michael Seidel
Small molecules like antibiotics or other pharmaceuticals can be detected and quantified, among others, with indirect competitive immunoassays. With regard to multiplex quantification, these tests can be performed as chemiluminescence microarray immunoassays, in which, in principle, the analyte in the sample and the same substance immobilized on the chip surface compete for a limited number of specific antibody binding sites. The amount of the specific primary antibody that has been bound to the surface is visualized by means of a chemiluminescence reaction...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27871082/self-reported-physical-activity-quality-of-life-and-psychological-status-in-relation-to-plasma-25-hydroxyvitamin-d-concentration-in-patients-treated-with-hemodialysis
#10
Alicja E Grzegorzewska, Agnieszka Izdebska, Leszek Niepolski, Wojciech Warchoł, Paweł P Jagodziński
BACKGROUND/AIMS: Vitamin D status is announced among factors that may influence physical performance and mental health. Our aim was to evaluate self-reported physical activity, quality of life, psychiatric functioning, and affects with respect to plasma 25-hydroxyvitamin D [25(OH)D] concentrations in HD patients. METHODS: The study was carried out in HD patients not receiving vitamin D supplements (n = 112). IPAQ-L, QLI-D, GHQ-28, and PANAS were used in psychological evaluations...
November 21, 2016: Kidney & Blood Pressure Research
https://www.readbyqxmd.com/read/27845789/simultaneous-detection-of-multiple-viruses-based-on-chemiluminescence-and-magnetic-separation
#11
Zeeshan Ali, Jiuhai Wang, Yongjun Tang, Bin Liu, Nongyue He, Zhiyang Li
Nucleic acid testing (NAT) based methods are more sensitive and specific, and are preferred over enzyme immunoassays. Different NAT based protocols have been designed to detect multiple pathogens in order to reduce the inherent high cost for detection. However, these assays do not reliably detect a large number of pathogens at once. In this report, a DNA hybridization based chemiluminescence detection method has been proposed for reliable detection of multiple pathogens. The idea was practically demonstrated by carrying out simultaneous extraction and amplification of hepatitis B virus (HBV), hepatitis C virus (HCV), and human immunodeficiency virus (HIV-1 group M, herein referred to as HIV) through the one step multiplex reverse transcription polymerase chain reaction (RT-PCR)...
November 15, 2016: Biomaterials Science
https://www.readbyqxmd.com/read/27816940/does-methylphenidate-reduce-testosterone-levels-in-humans-a-prospective-study-in-children-with-attention-deficit-hyperactivity-disorder
#12
Liang-Jen Wang, Miao-Chun Chou, Wen-Jiun Chou, Min-Jing Lee, Pao-Yen Lin, Sheng-Yu Lee, Yi-Hsuan Lee
BACKGROUND: Animal studies and case reports have suggested that methylphenidate (MPH) exerts adverse effects on gonadal hormones. This study aimed to determine whether MPH alters testosterone levels in children with attention-deficit/hyperactivity disorder (ADHD) through comparison of those with or without MPH treatment. METHODS: This 4-week, nonrandomized, prospective study conducted in Taiwan included 203 ADHD patients with a mean age of 8.7 years (boys: 75.8%)...
November 5, 2016: International Journal of Neuropsychopharmacology
https://www.readbyqxmd.com/read/27810706/how-to-measuring-blood-cytokines-in-biological-psychiatry-using-commercially-available-multiplex-immunoassays
#13
Raoul Belzeaux, Marie-Noëlle Lefebvre, Anne Lazzari, Tifenn Le Carpentier, Julia-Lou Consoloni, Xavier Zendjidjian, Mocrane Abbar, Philippe Courtet, Jean Naudin, José Boucraut, Pierre Gressens, Nicolas Glaichenhaus, El Chérif Ibrahim
Cytokines produced by both immune and non-immune cells are likely to play roles in the development and/or progression of psychiatric disorders. Indeed, many investigators have compared the blood cytokine levels in psychiatric patients with those of healthy controls or monitored their levels in patients during disease progression to identify biomarkers. Nevertheless, very few studies have confirmed that such cytokines remain stable in healthy individuals through periods of weeks and months. This is an important issue to consider before using blood cytokine levels as biomarkers of disease traits, disease state, or treatment response...
January 2017: Psychoneuroendocrinology
https://www.readbyqxmd.com/read/27798889/evaluation-of-an-automated-clia-system-for-the-determination-of-carbohydrate-antigen-19-9
#14
Emanuela Anastasi, Chantal DI Segni, Silvestra Tudini, Giuseppina Gennarini, Antonio Angeloni
AIM: To compare analytical characteristics between two different methods, a automated chemiluminescent immunoassay (CLIA) versus a radioimmunometric assay (RIA), for the determination of carbohydrate antigen 19-9 (CA19-9). MATERIALS AND METHODS: One hundred and eighty-five blood samples from consecutively enrolled individuals (87 males and 98 females, aged 22-89 and 29-89 years, respectively) were evaluated. For both assays, a cut-off of 37 U/ml was used. RESULTS: Comparison between assays was analyzed using Passing-Bablok regression, which showed a high inter-assay correlation (r=0...
October 2016: Anticancer Research
https://www.readbyqxmd.com/read/27788631/detection-of-occult-hepatitis-b-and-window-period-infection-among-blood-donors-by-individual-donation-nucleic-acid-testing-in-a-tertiary-care-center-in-south-india
#15
Cinzia S Keechilot, Veena Shenoy, Anil Kumar, Lalitha Biswas, Sukhithasri Vijayrajratnam, Kavitha Dinesh, Prem Nair
With the introduction of highly sensitive hepatitis B surface antigen immunoassay, transfusion associated HBV infection have reduced drastically but they still tend to occur due to blood donors with occult hepatitis B infection (OBI) and window period (WP) infection. Sera from, 24338 healthy voluntary blood donors were screened for HBsAg, HIV and HCV antibody using Vitros Enhanced Chemiluminescent Immunoassay. The median age of the donor population was 30 (range 18-54) with male preponderance (98%). All serologically negative samples were screened by nucleic acid testing (NAT) for viral DNA and RNA...
October 27, 2016: Pathogens and Global Health
https://www.readbyqxmd.com/read/27787390/high-25-hydroxyvitamin-d-is-associated-with-unexpectedly-high-plasma-inflammatory-markers-in-hiv-patients-on-antiretroviral-therapy
#16
Louie Mar A Gangcuangco, Lindsay B Kohorn, Dominic C Chow, Sheila M Keating, Philip J Norris, Lorna S Nagamine, Lishomwa C Ndhlovu, Scott A Souza, Kalpana J Kallianpur, Cecilia M Shikuma
Inflammation associated with low 25-hydroxyvitamin D (25(OH)D) is associated with increased morbidity and mortality among HIV-infected patients with vitamin D deficiency. We investigated the association between 25(OH)D and soluble biomarkers among HIV-infected patients on stable antiretroviral therapy. This is a cross-sectional study. This study focuses on assessment in subjects 40 years or older on stable antiretroviral therapy (ART) for >3 months. Chemiluminescent immunoassay was used to determine plasma 25(OH)D levels...
October 2016: Medicine (Baltimore)
https://www.readbyqxmd.com/read/27783125/chemiluminescence-lateral-flow-immunoassay-cartridge-with-integrated-amorphous-silicon-photosensors-array-for-human-serum-albumin-detection-in-urine-samples
#17
Martina Zangheri, Fabio Di Nardo, Mara Mirasoli, Laura Anfossi, Augusto Nascetti, Domenico Caputo, Giampiero De Cesare, Massimo Guardigli, Claudio Baggiani, Aldo Roda
A novel and disposable cartridge for chemiluminescent (CL)-lateral flow immunoassay (LFIA) with integrated amorphous silicon (a-Si:H) photosensors array was developed and applied to quantitatively detect human serum albumin (HSA) in urine samples. The presented analytical method is based on an indirect competitive immunoassay using horseradish peroxidase (HRP) as a tracer, which is detected by adding the luminol/enhancer/hydrogen peroxide CL cocktail. The system comprises an array of a-Si:H photosensors deposited on a glass substrate, on which a PDMS cartridge that houses the LFIA strip and the reagents necessary for the CL immunoassay was optically coupled to obtain an integrated analytical device controlled by a portable read-out electronics...
December 2016: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/27771345/the-association-between-vitamin-d-receptor-polymorphisms-and-serum-25-hydroxyvitamin-d-levels-with-ulcerative-colitis-in-chinese-han-population
#18
Shu-Zi Zheng, Da-Guan Zhang, Hao Wu, Li-Jia Jiang, Jie Jin, Xiu-Qing Lin, Ran Ding, Yi Jiang
There is now growing evidence suggesting that Vitamin D is playing a critical role in modulating the innate and adaptive immune responses. Several polymorphisms have been identified in the vitamin D receptor (VDR) gene but their association with ulcerative colitis (UC) susceptibility remained controversy. In the current study, we examined the association between VDR polymorphisms and serum level of 25-hydroxyvitamin D [25(OH)D] with UC in Chinese Han population. Polymorphisms of FokI (rs2228570)/BsmI (rs1544410)/ApaI (rs7975232)/TaqI (rs731236) in the VDR gene were assessed in a case-control study comprising 404 UC patients and 612 controls...
October 19, 2016: Clinics and Research in Hepatology and Gastroenterology
https://www.readbyqxmd.com/read/27767254/the-chemiluminescence-immunoassay-for-aflatoxin-b1-based-on-functionalized-magnetic-nanoparticles-with-two-strategies-of-antigen-probe-immobilization
#19
Manwen Yao, Linyu Wang, Chengzhi Fang
A rapid and sensitive chemiluminescence immunoassay (CLIA) based on magnetic nanoparticles (MNPs) was developed to detect aflatoxin B1 (AFB1), which is a potent carcinogen in nature. We prepared monodisperse MNPs (300 nm in diameter) according to the solvothermal synthesis reaction and the MNPs were coated with silica by the Stöber method. Triethox was used as a one-step carboxylation reagent, and 3-aminopropyltriethoxysilane (APTES) an amination reagent, to modify the MNPs. We prepared two types of solid phase antigens using the above synthesized functionalized MNPs coupled with the later prepared AFB1-oxime active ester and the purchased BSA-AFB1 respectively...
October 21, 2016: Luminescence: the Journal of Biological and Chemical Luminescence
https://www.readbyqxmd.com/read/27766170/laboratory-experience-with-the-liaison-analyzer-in-the-diagnosis-of-clostridium-difficile-associated-diarrhea
#20
Orsolya Benedek, Andreas Podbielski, Philipp Warnke
BACKGROUND: Chemiluminescent or enzyme-linked fluorescent immunoassays are commonly used to diagnose Clostridium difficile-associated diarrhea. METHODS: The LIAISON analyzer (DiaSorin, Italy) was compared to miniVIDAS (bioMérieux, France) and, furthermore, to culture of toxigenic strains. In total, 249 native stool samples were analyzed. Sensitivities, specificities, and positive and negative predictive values were investigated. Furthermore, performance under routine conditions was assessed...
September 29, 2016: European Journal of Microbiology & Immunology
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