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chemiluminescence immunoassay

Henry H Nguyen, Abdel Aziz Shaheen, Natalia Baeza, Ellina Lytvyak, Stefan J Urbanski, Andrew L Mason, Gary L Norman, Marvin J Fritzler, Mark G Swain
BACKGROUND AND AIMS: Up to 20% of Primary Biliary Cholangitis (PBC) patients are estimated to have features that overlap with Autoimmune Hepatitis (AIH). Patients with PBC-AIH overlap syndrome (PBC-AIH OS) have been reported to exhibit suboptimal responses to ursodeoxycholic acid therapy, and are more likely to progress to cirrhosis. Anti-double stranded DNA (anti-dsDNA) and anti-p53 have been previously suggested to be potential autoantibodies for identifying patients with PBC-AIH OS...
2018: PloS One
Alessia Gloria, Alberto Contri, Augusto Carluccio, Domenico Robbe
Blood progesterone concentration is used in several procedures related to the reproduction in the bitch, such as ovulation monitoring, estimating time of parturition, or hypo-luteoidism management. Several techniques are available to evaluate blood progesterone concentration, such as the radioimmunoassay (RIA), the chemiluminescent immunoassay (CLIA), and the enzyme-linked immunosorbent assay (ELISA). The aim of this study was to compare the blood progesterone concentration using these three methods during the periovulatory period of 23 bitches...
March 7, 2018: Animal Reproduction Science
Hiroyuki Takahama, Seiji Takashio, Toshio Nishikimi, Tomohiro Hayashi, Chiaki Nagai-Okatani, Yasuaki Nakagawa, Makoto Amaki, Takahiro Ohara, Takuya Hasegawa, Yasuo Sugano, Hideaki Kanzaki, Satoshi Yasuda, Kenji Kangawa, Naoto Minamino, Toshihisa Anzai
BACKGROUND: A recent study showed that both glycosylation of pro-B-type natriuretic peptide (BNP) and the proBNP/total BNP ratio are decreased in acute decompensated heart failure (ADHF). However, the following points regarding the proBNP/total BNP ratio have not been determined in patients with ADHF: 1) the relationship with the severity of ADHF, 2) the changes in the ratio during treatment, and 3) the relationship with cyclic guanosine monophosphate (cGMP)-generating activity. METHODS: Plasma proBNP and total BNP (proBNP+mature BNP) were measured in patients with ADHF (n=154)...
May 1, 2018: International Journal of Cardiology
Christina Boeck, Sophia Pfister, Alexander Bürkle, Valerie Vanhooren, Claude Libert, Juan Salinas-Manrique, Detlef E Dietrich, Iris-Tatjana Kolassa, Alexander Karabatsiakis
BACKGROUND: Glycans are short chains of saccharides linked to glycoproteins that are known to be involved in a wide range of inflammatory processes. As depression has been consistently associated with chronic low-grade inflammation, we asked whether patients with Major Depressive Disorder show alterations in the N-glycosylation pattern of serum proteins that might be linked to associated changes in inflammatory processes. METHODS: In a study cohort of 21 female patients with an acute depressive episode and 21 non-depressed female control subjects aged between 50 and 69 years, we analyzed the serum N-glycan profile by DNA Sequencer Adapted-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) and assessed the serum levels of interleukin (IL)- 6, tumor necrosis factor (TNF)-α and C-reactive protein (CRP) by chemiluminescence immunoassays and nephelometry...
February 27, 2018: Journal of Affective Disorders
Robert J Kemppainen, Ellen N Behrend, Stephanie F Carter, Janeva E Cole
Hormone assays that use a solid-phase, automated, chemiluminescent enzyme immunoassay (CEIA) with an alkaline phosphatase-tagged hormone or antibody as a reporter are performed on serum or EDTA plasma in our laboratory. CEIA cortisol results appeared to increase in the presence of excess EDTA. We investigated the effect of the addition of different amounts of EDTA on cortisol concentrations in pooled canine serum samples. The recommended EDTA plasma concentration of 4.1 mmol/L (1.8 mg/mL) did not alter cortisol concentrations when added to serum pools; however, the addition of ≥5...
March 1, 2018: Journal of Veterinary Diagnostic Investigation
Jolien Claessens, Thibaut Belmondo, Ellen De Langhe, Rene Westhovens, Koen Poesen, Sophie Hüe, Daniel Blockmans, Michael Mahler, Marvin J Fritzler, Xavier Bossuyt
Solid phase assays (SPAs) and automated microscope systems are increasingly used to screen for antinuclear antibodies (ANAs). The goal of this study was to evaluate the performance of three automated ANA screening assays; NOVA Lite HEp-2 using NOVA View® (NV, Inova Diagnostics), an automated indirect immunofluorescence method, EliA™ CTD Screen (Fluorescence Enzyme Immunoassay, FEIA; Thermo Fisher) and QUANTA Flash® CTD Screen Plus (Chemiluminescence immunoassay, CIA; Inova Diagnostics). The assays were performed on 480 diagnostic samples from patients with an ANA-associated rheumatic disease (AARD; systemic lupus erythematosus, primary Sjögren's syndrome, systemic sclerosis, inflammatory myopathy, mixed connective tissue disease) and on 767 samples from diseased and healthy controls...
March 8, 2018: Autoimmunity Reviews
Xiaojuan Ren, Hui Zhang, Hui Cong, Xudong Wang, Hongbing Ni, Xianjuan Shen, Shaoqing Ju
Epithelium ovarian cancer (EOC) is currently the prevalent malignant cancer worldwide. However, there is a lack of efficient biomarkers for EOC screening. Accumulating evidence reveals that serum miRNA detectable in various types of cancer. Therefore, we explore the diagnostic value of combined detection of plasma miR-193a-5p, HE4 and CA125 for EOC. Serum samples were collected from 45 patients with primary EOC, 30 patients with benign ovarian tumor patients and 40 healthy controls. The expression of serum miR-193a-5p was detected by real-time quantitative PCR, and serum HE4 and CA125 were detected by chemiluminescent immunoassay...
March 8, 2018: Pathology Oncology Research: POR
R Jhorawat, N Bansal, P Beniwal, D Agarwal, V Malhotra
The aim of this study was to measure insulin resistance (IR) in dialysis patients and its relation to Vitamin D status and nutritional parameters. We included patients on maintenance dialysis, both hemodialysis and peritoneal dialysis (HD and PD). IR was measured by homeostatic model assessment (HOMA)-IR index defined as fasting serum insulin (μU/L) × fasting blood sugar (mmol/L)/22.5. Baseline Vitamin D levels were measured by chemiluminescence immunoassay (CLIA) method. HOMA-IR index correlated with nutritional parameters such as 7-point subjective global assessment (SGA) and anthropometric measures, for example, body fat percentage, lean body weight (LBW), mid-arm circumference (MAC), and mid-arm muscle circumference (MAMC)...
January 2018: Indian Journal of Nephrology
Hidetoshi Takedatsu, Keiichi Mitsuyama, Shuhei Fukunaga, Shinichiro Yoshioka, Ryosuke Yamauchi, Atsushi Mori, Hiroshi Yamasaki, Kotaro Kuwaki, Hideto Sakisaka, Shotaro Sakisaka, Takuji Torimura
BACKGROUND AND AIM: Proteinase 3 antineutrophil cytoplasmic antibodies (PR3-ANCAs) are well-known serological markers for granulomatosis with polyangiitis, but their role as serological markers for inflammatory bowel disease (IBD) remains uncertain. The present study aimed to evaluate the diagnostic and clinical role of PR3-ANCAs as markers for IBD. METHODS: Using a new methodology with chemiluminescence enzyme immunoassay, serum PR3-ANCA titres were assessed in 102 patients with ulcerative colitis (UC), 67 patients with Crohn's disease (CD), 44 controls with other intestinal diseases and 66 healthy controls...
March 7, 2018: Journal of Gastroenterology and Hepatology
Małgorzata Jamka, Anita Arslanow, Annika Bohner, Marcin Krawczyk, Susanne N Weber, Frank Grünhage, Frank Lammert, Caroline S Stokes
BACKGROUND/AIMS: Common genetic variations in vitamin D metabolism are associated with liver stiffness. Whether these genes are implicated in hepatic steatosis remains unclear. Here we aimed to analyse the association of common vitamin D pathway gene variants with liver steatosis. METHODS: Liver steatosis was assessed non-invasively in 241 patients with chronic liver conditions by controlled attenuation parameter (CAP). The following polymorphisms were genotyped using TaqMan assays: group-specific component (GC) rs7041, 7-dehydrocholesterol reductase (DHCR7) rs12785878, cytochrome P450 2R1 (CYP2R1) rs10741657, -vitamin D receptor (VDR) rs7974353...
March 7, 2018: Digestion
Jonathan Vogelgsang, Jens Wiltfang, Hans W Klafki
The technical performance of immunological assays and their suitability for the intended use should be carefully validated before implementation in research, clinical studies or routine. We describe here the evaluation of a sandwich electrochemiluminescence immunoassay for measuring total Amyloid-β levels in human blood plasma as an example of a laboratory protocol for a partial "fit for purpose" assay performance validation. We tested two different assay protocols and addressed impact of sample dilution, parallelism, intra- and inter-assay variance, lower limit of quantification, lower limit of detection, and analytical spike recoveries...
2018: Methods in Molecular Biology
Vladislava T Djurasinović, Biljana S Mihaljević, Sandra B Šipetić Grujičić, Svetlana D Ignjatović, Goran Trajković, Milena R Todorović-Balint, Darko A Antić, Jelena S Bila, Boško M Andjelić, Jelena J Jeličić, Vojin M Vuković, Aleksandra M Nikolic, Stanislaw Klek
INTRODUCTION: Vitamin D has a role in cellular differentiation, proliferation, apoptosis, and angiogenesis and therefore is studied as a prognostic factor in cancer. The aim of our study was to assess the prevalence and significance of 25(OH)D deficiency in patients with lymphoid malignancies. METHODOLOGY: Between January 2014 and June 2016 at the Clinic for Hematology, Clinical Center of Serbia, Belgrade, the pretreatment serum level of 25(OH)D was determined in 133 (62 women/71 men, median age 58 (18-84) years) previously untreated patients with lymphoid malignancy using a chemiluminescent immunoassay...
March 6, 2018: Supportive Care in Cancer: Official Journal of the Multinational Association of Supportive Care in Cancer
Victoria Ortiz de la Tabla, Marta Berruezo, Elena García Payá, Marta Fernández, José Alberto García, Mar Masiá, Félix Gutiérrez
BACKGROUND: Mycoplasma pneumoniae is considered an important etiologic agent of community-acquired pneumonia (CAP) in outpatients. We aimed to evaluate the diagnostic accuracy of a quick automated chemiluminescent immunoassay (CLIA) for M. pneumoniae in a population-based prospective study of CAP. METHODS: A total of 137 outpatients diagnosed with CAP were included in the study. Acute- and convalescent phase sera were analyzed for IgG and IgM to M. pneumoniae with both CLIA (VirClia® ) and ELISA immunoassays...
March 6, 2018: Journal of Clinical Laboratory Analysis
Juan C Domínguez-Romero, Bienvenida Gilbert-López, Miriam Beneito-Cambra, Antonio Molina-Díaz
In this work, the implementation of Bead Injection with multicommutation-based flow systems is reported. A surface renewable chemiluminescence (CL) flow sensor is presented based on the use of CL reaction of luminol with H2 O2 . Dowex 1 × 8 beads with immobilized luminol onto them were injected in the flow system by means of a six-port rotary valve and were accommodated into a 1 mm optical glass flow cell placed just in front of the rectangular photosensor window with the same size than the cell wall. Automatic computer-controlled manipulation of both reagents and sample solutions was undertaken using a multicommutated flow system which comprises five three-way solenoid valves, a home-made electronic interface and a Java-written software...
May 15, 2018: Talanta
Timur Ekiz, Serkan Fatih Yeğen, Mehmet Kağan Katar, Ömer Genç, Selin Genç
This study compared the 25-hyrdoxyvitamin (OH) D and bone mineral density (BMD) values of patients with and without cholecystectomy. Although 25(OH) D levels were significantly lower in the cholecystectomy group (12.1 ± 6.2 vs. 15.6 ± 6.6 ng/mL), no significant difference was observed between the groups in terms of BMD measurements. INTRODUCTION: Although 25 (OH) D levels were studied and found to be lower in patients with cholecystectomy, the data is scarce as regards the BMD...
March 2, 2018: Archives of Osteoporosis
Binbin Song, Xia Li, Qiang Zhou, Xinmei Yang, Yiming Jiang, Aifen Wang
BACKGROUND: This study is to investigate the application of the bone turnover markers type I procollagen carboxyterminal propeptide (PICP) and β-isomerized forms of type I collagen breakdown products (β-CTx) in the diagnosis and treatment of breast cancer with bone metastases. METHODS: A total of 162 breast cancer patients were included in this study. There were 70 cases with bone metastasis (BM group) and 92 cases without bone metastasis (non-bone metastasis, NBM group)...
January 1, 2018: Clinical Laboratory
Rafael Benito, Jorge Arribas, Sonia Algarate, Rocío Cebollada, M José Gude
Organ donors and recipients are routinely screened for hepatitis C virus (HCV) infection, typically via anti-HCV detection. We analyze the utility of an alternative HCV core antigen (HCV-Ag) quantification system, the ARCHITECT HCV Ag Assay, in this setting. We simultaneously tested 315 samples from potential organ donors and recipients using two chemiluminescent microparticle immunoassays: ARCHITECT Anti-HCV and HCV Ag (Abbott, Germany). HCV-Ag was detected in 81 of the serum samples (25.71%) and anti-HCV in 87 (27...
February 2, 2018: Diagnostic Microbiology and Infectious Disease
Leila Nikniaz, Masood Shirmohammadi, Jafar Sadegh Tabrizi, Homayoun Sadeghi Bazargani, Mostafa Farahbakhsh
OBJECTIVE: The relationship between serum 25-hydroxy-vitamin D and within normal range liver enzymes in a population-based study in the northwest of Iran was investigated. METHOD: This cross-sectional study was comprised of 700 apparently healthy Iranian adults (287 men & 413 women) who participated in the major lifestyle promotion project (LPP) conducted in East Azarbaijan-Iran in 2015. The ultraviolet method and chemiluminescent immunoassay technology were respectively used for determination of the serum levels of liver enzymes (alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) and 25-hydroxy-vitamin D...
February 22, 2018: International Journal for Vitamin and Nutrition Research. Journal International de Vitaminologie et de Nutrition
Meng-Lan Wang, Juan Liao, Bing Wei, Dong-Mei Zhang, Ming He, Ming-Chuan Tao, En-Qiang Chen, Hong Tang
AIM: Recent studies revealed that both quantitative hepatitis B surface antigen (qHBsAg) and hepatitis B core-related antigen (qHBcrAg) could serve as a good marker for predicting treatment response and indirectly reflecting intrahepatic cccDNA levels. This study aimed to compare the value of qHBsAg and qHBcrAg in predicting HBeAg seroconversion among patients undergoing PEG-IFN therapy. METHODS: A total of 31 HBeAg-positive patients, who underwent PEG-IFN therapy for 12 months and follow-up for six months were retrospectively included in this study...
February 20, 2018: Infectious Diseases
Chaochao Chen, Jiaxun Luo, Chenglong Li, Mingfang Ma, Wenbo Yu, Jianzhong Shen, Zhanhui Wang
The chemical contaminants in food and environment are quite harmful to food safety and human health. Rapid, accurate and cheap detection can effectively control the potencial risk derived from these chemical contaminants. Among all detection methods, the immunoassay based on specific interaction of antibodies-analyte is one of the most widely used technique in the field. However, biological antibodies employed in immunoassay usually cannot tolerate extreme conditions, resulting in unstable state in both physical and chemical profiles...
February 20, 2018: Journal of Agricultural and Food Chemistry
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