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https://www.readbyqxmd.com/read/28912096/analytical-verification-and-method-comparison-of-the-advia-centaur%C3%A2-intact-parathyroid-hormone-assay
#1
Esther Fernández, Josep Lluís Bedini, Xavier Filella
OBJECTIVES: This study is the first verification of the novel iPTH Siemens ADVIA Centaur® Intact Parathyroid Hormone (iPTHm) chemiluminescence immunoassay based on monoclonal antibodies. We also compared the iPTH results obtained using this assay with the previous ADVIA Centaur® Parathyroid Hormone assay (iPTHp) based on polyclonal antibodies. DESIGN AND METHODS: The analytical performance study of iPTHm assay included LoD, LoQ, intra- and inter-assay reproducibility, and linearity...
September 11, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28911998/establishing-age-specific-reference-intervals-for-anti-m%C3%A3-llerian-hormone-in-adult-chinese-women-based-on-a-multicenter-population
#2
Xinqi Cheng, Qiong Zhang, Min Liu, Shijun Li, Zhihua Tao, Kiyoshi Ichihara, Songlin Yu, Kai Zhang, Pengchang Li, Jianhua Han, Ling Qiu
AIM OF THIS STUDY: Anti-Müllerian hormone (AMH) is useful for the assessment of ovarian reserve and treatment of individualized in vitro fertilization (IVF). The aim of this study is to establish AMH reference interval for adult Chinese women on the Beckman Beckman DxI 800 platform. MATERIAL AND METHODS: From May to September 2013, serum from 1169 apparently healthy adult females from five representative cities in China (Beijing, Hangzhou, Guangzhou, Dalian and Urumqi) were collected, and AMH was analyzed on the platform of Beckman DxI 800 automated chemiluminescence immunoassay...
September 12, 2017: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/28905534/larger-testicular-volume-is-independently-associated-with-favorable-indices-of-lung-function
#3
Tae Beom Kim, I Nae Park
BACKGROUND: Men with chronic obstructive pulmonary disease, have reduced endogenous testosterone levels, but the relationship between pulmonary function and endogenous testosterone levels, is inconsistent. Testicular volume is a known indicator of endogenous testosterone levels, male fertility, and male potency. In the present study, the authors investigated the relationship, between testicular volume and lung function. METHODS: One hundred and eighty-one South Korean men age 40-70, hospitalized for urological surgery, were retrospectively enrolled, irrespective of the presence of respiratory disease...
September 1, 2017: Tuberculosis and Respiratory Diseases
https://www.readbyqxmd.com/read/28891673/correlation-between-serum-lead-and-thyroid-diseases-papillary-thyroid-carcinoma-nodular-goiter-and-thyroid-adenoma
#4
Hui Li, Xiang Li, Jie Liu, Langping Jin, Fan Yang, Junbo Wang, Ouchen Wang, Ying Gao
Studies have showed that lead was associated with human health. However, the effects of lead on thyroid functions are inconsistent, and studies based on Chinese population are fragmentary. To evaluate the correlation between lead and thyroid functions of Chinese with different thyroid diseases, we conducted a hospital-based study. Ninety-six papillary thyroid carcinoma (PTC), 10 nodular goiter (NG), and 7 thyroid adenoma (TA) patients were recruited from the First Affiliated Hospital of Wenzhou Medical University, China...
September 11, 2017: International Journal of Environmental Health Research
https://www.readbyqxmd.com/read/28891077/a-comparison-of-the-effects-of-cyp3a5-polymorphism-on-tacrolimus-blood-concentrations-measured-by-4-immunoassay-methods-in-renal-transplant-patients
#5
Yumiko Akamine, Hideaki Kagaya, Tadashi Ohkubo, Shigeru Satoh, Masatomo Miura
WHAT IS KNOWN AND OBJECTIVE: The anti-tacrolimus antibodies used in commercial immunoassay methods have cross-reactivity with tacrolimus metabolites. The aim of this study was to investigate differences in the effects of CYP3A5 polymorphism on tacrolimus concentrations obtained by four immunoassay methods in renal transplant patients. METHODS: Samples (n = 508) were evaluated using four immunoassays (chemiluminescence enzyme immunoassay [CLIA], affinity column-mediated immunoassay [ACMIA], electrochemiluminescence immunoassay [ECLIA] and latex agglutination turbidimetric immunoassay [LTIA])...
September 10, 2017: Journal of Clinical Pharmacy and Therapeutics
https://www.readbyqxmd.com/read/28881814/diagnostic-and-prognostic-values-of-cerebrospinal-fluid-cyfra-21-1-in-patients-with-leptomeningeal-carcinomatosis
#6
Jae-Won Hyun, Ji Hyun Park, Boo Gil Kang, Eun Young Park, Boram Park, Jungnam Joo, Jong Kuk Kim, Su-Hyun Kim, Jee Hyang Jeong, Hyang Woon Lee, Kee Duk Park, Kyung Gyu Choi, Sang-Hyun Hwang, Ho-Shin Gwak, Ho Jin Kim
OBJECTIVES: To investigate the diagnostic and prognostic values of cerebrospinal fluid (CSF) CYFRA 21-1 in patients with leptomeningeal carcinomatosis (LMC). METHODS: Concentration of CSF CYFRA 21-1 was detected using electro-chemiluminescent immunoassay. The difference in level of CYFRA 21-1 between 61 patients with LMC and 200 patients with other neurological disease was evaluated, and diagnostic performance of CSF CYFRA 21-1 was investigated. In LMC patients treated with ventriculo-lumbar perfusion (VLP) chemotherapy, prognostic performance of CSF CYFRA 21-1 was evaluated...
August 8, 2017: Oncotarget
https://www.readbyqxmd.com/read/28878003/evaluation-of-the-lumipulse%C3%A2-g-tp-n-chemiluminescent-immunoassay-as-a-syphilis-screening-test
#7
Daniel A Ortiz, Michael J Loeffelholz
A syphilis diagnosis is often aided by the detection of treponemal and non-treponemal antibodies. Automated treponemal antibody detection systems enable high-volume clinical laboratories to perform syphilis screening at a faster pace with lower labor costs. The Lumipulse G TP-N chemiluminescent immunoassay is an automated system that qualitatively detects IgG and IgM antibodies against Treponema pallidum antigens in human serum and plasma. To assess performance characteristics and workflow efficiency, the Lumipulse G TP-N assay was compared to the Bioplex 2200 Syphilis IgG multiplex flow immunoassay...
September 6, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28872086/-subclinical-hypothyroidism-and-endocrine-metabolic-characteristics-in-women-with-polycystic-ovary-syndrome
#8
Fangzhi Yue, Dongmei Zhang, Fei Gong, Li Zhang, Zhixiang Sun, Minxiang Lei
To explore associations of subclinical hypothyroidism (SCH) with endocrine metabolic characteristics in women with polycystic ovary syndrome (PCOS).
 Methods: A total of 321 women who were newly diagnosed as PCOS were recruited from two endocrine outpatient clinics. The diagnosis of PCOS was established according to the 2003 Rotterdam consensus criteria. Thyroid function was examined by chemiluminescent immunoassay. Patients who had normal free thyroxine (FT4) were divided into different SCH subgroups according to two thyroid stimulating hormone (TSH) cutoff points (4...
August 28, 2017: Zhong Nan da Xue Xue Bao. Yi Xue Ban, Journal of Central South University. Medical Sciences
https://www.readbyqxmd.com/read/28870829/chemiluminescence-immunoassays-for-estradiol-and-ethinylestradiol-based-on-new-biotinylated-estrogen-derivatives
#9
Hussein Kanso, Nicolas Inguimbert, Georges Istamboulie, Lise Barthelmebs, Carole Calas-Blanchard, Thierry Noguer
New chemiluminescence-based immunoassays for sensitive detection of 17-β estradiol (E2) and ethinylestradiol (EE2) are described on the basis of the use of biotinylated estrogen derivatives. Estrogen derivatives bearing a carboxylic group (E2-COOH and EE2-COOH) on C-3 position were synthesized, covalently bound to aminated biotin and subsequently immobilized on avidin-coated microtiter plates. The assay principle was based on competition between free and immobilized estrogens for their binding to primary antibodies, with subsequent revelation using horseradish peroxidase (HRP)-labeled secondary antibodies...
September 7, 2017: Analytical Biochemistry
https://www.readbyqxmd.com/read/28864194/cathodic-electrogenerated-chemiluminescence-of-aromatic-tb-iii-chelates-at-polystyrene-graphite-composite-electrodes
#10
Kalle Salminen, Päivi Grönroos, Sami Tuomi, Sakari Kulmala
Tb(III) chelates exhibit intense hot electron-induced electrogenerated chemiluminescence during cathodic polarization of metal/polystyrene-graphite (M/PG) electrodes in fully aqueous solutions. The M/PG working electrode provides a sensitive means for the determination of aromatic Tb(III) chelates at nanomolar concentration levels with a linear log-log calibration curve spanning more than five orders of magnitude. The charge transport and other properties of these novel electrodes were studied by electrochemiluminescence measurements and cyclic voltammetry...
September 8, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28863782/effectiveness-of-pivka-ii-in-the-detection-of-hepatocellular-carcinoma-based-on-real-world-clinical-data
#11
Rentao Yu, Zhaoxia Tan, Xiaomei Xiang, Yunjie Dan, Guohong Deng
BACKGROUND: Protein Induced by Vitamin K Absence or Antagonist-II (PIVKA-II) is an efficient biomarker specific for hepatocellular carcinoma (HCC). Some researchers have proved that levels of PIVKA-II reflect HCC oncogenesis and progression. However, the effectiveness of PIVKA-II based on real-world clnical data has barely been studied. METHODS: A total of 14,861 samples were tested in Southwest Hospital in over 2 years' time. Among them, 4073 samples were PIVKA-II positive...
September 1, 2017: BMC Cancer
https://www.readbyqxmd.com/read/28856221/analytical-performance-evaluation-of-the-elecsys%C3%A2-cyclosporine-and-elecsys%C3%A2-tacrolimus-assays-on-the-cobas-e411-analyzer
#12
Maki Sasano, Shigeki Kimura, Ikuhiro Maeda, Yoh Hidaka
BACKGROUND: Cyclosporine (CsA) and tacrolimus (TAC) are immunosuppressant drugs that are often used to treat autoimmune diseases and as transplantation therapy; therefore, their concentrations need to be monitored carefully. We herein evaluated the analytical performance of the Elecsys® Cyclosporine and Elecsys® Tacrolimus assay kits, which have been newly developed to measure CsA and TAC concentrations in the whole blood. METHODS: We used residual whole blood samples from autoimmune disease and transplantation patients who were being treated with CsA or TAC...
August 2017: Pract Lab Med
https://www.readbyqxmd.com/read/28852111/development-of-an-affimer-antibody-combined-immunological-diagnosis-kit-for-glypican-3
#13
Chunmei Xie, Christian Tiede, Xuanyi Zhang, Congrong Wang, Zhixiong Li, Xiao Xu, Michael J McPherson, Darren C Tomlinson, Weiwen Xu
Glypican-3 (GPC3) is a promising new marker for hepatocellular carcinoma, but the reported values for serum GPC3 differ markedly between currently available kits. Here we isolated Affimer non-antibody binding proteins against GPC3 by phage display and developed a new sandwich chemiluminescence immunoassay (CLIA) combining an Affimer with a monoclonal antibody (Affimer-MAb CLIA). The proposed CLIA assay demonstrated a wide linear range  0.03-600 ng/mL) with a good linear correlation coefficient (0.9999), a high detection limitation (0...
August 29, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28851333/a-gonadotropin-releasing-hormone-antagonist-reduces-serum-adrenal-androgen-levels-in-prostate-cancer-patients
#14
Yoshiyuki Miyazawa, Yoshitaka Sekine, Takahiro Syuto, Masashi Nomura, Hidekazu Koike, Hiroshi Matsui, Yasuhiro Shibata, Kazuto Ito, Kazuhiro Suzuki
BACKGROUND: Adrenal androgens play an important role in the development of castration-resistant prostate cancer therapeutics. The effect of gonadotropin-releasing hormone (GnRH) antagonists on adrenal androgens has not been studied sufficiently. We measured testicular and adrenal androgen levels in patients treated with a GnRH antagonist. METHODS: This study included 47 patients with histologically proven prostate cancer. All of the patients were treated with the GnRH antagonist degarelix...
August 29, 2017: BMC Urology
https://www.readbyqxmd.com/read/28844341/comparing-three-treponemal-tests-for-syphilis-screening
#15
Sean A Buono, Hilary A Godwin, Nicole M Green
We compared the performance and ease of use for three high-throughput treponemal immunoassays: Phoenix Biotech Trep-Sure Total Antibody EIA, Siemens ADVIA® Centaur Syphilis Assay, and DiaSorin LIAISON® Treponema Assay. One thousand serum samples submitted for routine screening were used in this study. Each assay demonstrated comparable sensitivity, specificity, and percent agreement (98-100%) compared with Treponema pallidum particle agglutination (TP-PA). Thus, treponemal immunoassays are an acceptable alternative for syphilis screening or confirmatory testing...
July 26, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28843825/implementation-of-a-rapid-hit-immunoassay-at-a-university-hospital-retrospective-analysis-of-hit-laboratory-orders-in-patients-with-thrombocytopenia
#16
Anne Black, Susanne Heimerl, Linnéa Oertli, Wolf Wilczek, Andreas Greinacher, Michael Spannagl, Wolfgang Herr, Christina Hart
BACKGROUND: Heparin-induced thrombocytopenia (HIT) is a rare cause of thrombocytopenia and a potentially life-threatening adverse drug reaction. Clinical overdiagnosis of HIT results in costly laboratory tests and anticoagulation. Criteria and algorithms for diagnosis are established, but their translation into clinical practice is still challenging. STUDY DESIGN AND METHODS: In a retrospective approach we studied all HIT related laboratory test requests within four years and evaluated data before (1st period, 24month) and after (2nd period, 24month) replacing particle gel immunoassay (PaGIA) and enzyme-linked immunosorbent assay (ELISA) by a chemiluminescent immunoassay (CLIA)...
August 15, 2017: Thrombosis Research
https://www.readbyqxmd.com/read/28842664/evaluation-of-the-elecsys-syphilis-immunoassay-for-routine-screening-of-serum-samples-in-china
#17
Chuanmin Tao, Xiaoke Hao, Wei Xu, Jie Zhang, Shiyang Pan, Zhihua Tao, Xiaofei Li, Junmei Chen, Bingchang Zhang, Yurong Qiu, Yanan Wu, Qishui Ou, Xianzhang Huang, Lanlan Wang
We compared the performance of the Roche Diagnostics Elecsys immunoassay for the detection of Treponema pallidum specific antibodies in patient serum samples with that of the Abbott Laboratories Architect chemiluminescent microparticle immunoassay and the InTec and KHB enzyme-linked immunosorbent assays, which are commonly used in China. We tested 13,767 serum samples collected from 13 independent laboratories throughout China, which included samples from 999 previously confirmed syphilis cases and 158 'borderline' samples previously identified using the Architect, InTec, and KHB tests...
August 25, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28840992/clinical-usefulness-of-human-epididymis-protein-4-in-lung-cancer
#18
Soo In Choi, Mi Ae Jang, Byung Ryul Jeon, Hee Bong Shin, You Kyoung Lee, Yong Wha Lee
Human epididymis protein 4 (HE4) has been suggested as a useful new biomarker of lung cancer; however, few relevant large-scale studies have been published. In this study, we evaluated the utility of serum HE4 for lung cancer detection. HE4 levels were measured in serum samples from 100 lung cancer patients, 57 patients with benign lung diseases, and 274 healthy controls by using a chemiluminescent immunoassay, and variations in HE4 levels were analyzed by clinical status such as lung cancer, benign lung disease, and healthy condition, Tumor, Lymph Nodes, Metastasis (TNM) stage, tumor score, and histological cancer type...
November 2017: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/28840989/comparison-of-traditional-and-reverse-syphilis-screening-algorithms-in-medical-health-checkups
#19
Eun Hee Nah, Seon Cho, Suyoung Kim, Han Ik Cho, Jong Yil Chai
BACKGROUND: The syphilis diagnostic algorithms applied in different countries vary significantly depending on the local syphilis epidemiology and other considerations, including the expected workload, the need for automation in the laboratory and budget factors. This study was performed to investigate the efficacy of traditional and reverse syphilis diagnostic algorithms during general health checkups. METHODS: In total, 1,000 blood specimens were obtained from 908 men and 92 women during their regular health checkups...
November 2017: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/28834954/low-varicella-zoster-virus-seroprevalence-among-young-children-in-the-democratic-republic-of-the-congo
#20
Reena H Doshi, Vivian H Alfonso, Patrick Mukadi, Nicole A Hoff, Sue Gerber, Ado Bwaka, Stephen G Higgins, Brian Cowell, Guillaume Ngoie Mwamba, Emile Okitolonda, Jean-Jacques Muyembe-Tamfum, Anne W Rimoin
BACKGROUND: Varicella zoster virus (VZV) causes both varicella (chickenpox) and herpes zoster (shingles) and is associated with significant global morbidity. Most epidemiological data on VZV come from high-come countries and to date there are limited data on the burden of VZV in Africa. METHODS: We assessed the seroprevalence of VZV antibodies among children in the Democratic Republic of Congo in collaboration with the 2013-2014 Demographic and Health Survey (DHS)...
August 19, 2017: Pediatric Infectious Disease Journal
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