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chemiluminescence immunoassay

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https://www.readbyqxmd.com/read/28734353/streptavidin-biotin-peroxidase-nanocomplex-amplified-microfluidics-immunoassays-for-simultaneous-detection-of-inflammatory-biomarkers
#1
Jing Wu, Yiping Chen, Mingzhu Yang, Yu Wang, Cheng Zhang, Mo Yang, Jiashu Sun, Mengxia Xie, Xingyu Jiang
Simultaneous, sensitive and quantitative detection of biomarkers in infectious disease is crucial for guiding antimicrobial treatment and predicting prognosis. This work reported an ultrasensitive and quantitative microfluidic immunoassay combined with the streptavidin-biotin-peroxidase (SA-B-HRP) nanocomplex-signal amplification system (MIS) to detect two inflammatory biomarkers, procalcitonin (PCT, for discriminating bacterial infections from nonbacterial infections) and interleukin-6 (IL-6, for monitoring the kinetics of infectious disease) simultaneously...
August 22, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28728299/-the-value-of-b7-h4-and-carcinoembryonic-antigen-in-diagnosing-the-benign-and-malignant-pleural-effusion
#2
F Wei, Y Wei, L F Li, G L Li, G J Wang
Objective: To evaluate the value of combined detection of negative costimulatory molecule B7-H4 and carcinoembryonic antigen (CEA) in diagnosing malignant and benign pleural effusion. Methods: Ninety-seven pleural effusion specimen were collected, 55 of which were diagnosed as malignant pleural effusion and 42 were benign pleural effusion. Enzyme-linked immunosorbent assay(ELISA) was used to examine the concentration of B7-H4 and CEA in pleural effusion. Electro-chemiluminescence immunoassay was used to detect the CEA level in pleural effusion...
July 23, 2017: Zhonghua Zhong Liu za Zhi [Chinese Journal of Oncology]
https://www.readbyqxmd.com/read/28724170/circulating-insulin-like-growth-factor-1-and-insulin-like-growth-factor-binding-protein-3-predict-three-months-outcome-after-ischemic-stroke
#3
Moritz Armbrust, Hans Worthmann, Reinhard Dengler, Helmut Schumacher, Ralf Lichtinghagen, Christoph Cyrill Eschenfelder, Matthias Endres, Martin Ebinger
Reports on neuroprotective effects of Insulin-like growth factor-1 (IGF-1) and Insulin-like growth factor binding protein-3 (IGFBP-3) in ischemic brain tissue are inconsistent. The aim of this study was to determine if plasma levels of IGF-1 and IGFBP-3 in acute stroke patients are indicative of 3 months functional outcome. Plasma levels were measured via chemiluminescence immunoassay in heparin blood samples of patients included in the EARLY trial (NCT00562588). Plasma samples were drawn on admission and 8 days post-stroke...
July 2017: Experimental and Clinical Endocrinology & Diabetes
https://www.readbyqxmd.com/read/28721140/serum-adipokines-and-vitamin-d-levels-in-patients-with-type-1-diabetes-mellitus
#4
Mohamed M Ismail, Tamer A Abdel Hamid, Alshaymaa A Ibrahim, Huda Marzouk
INTRODUCTION: Adiponectin, leptin and resistin are adipokines that play important roles in the regulation of lipid and carbohydrate metabolism in type 2 diabetes (T2DM). However, their influence in type 1 diabetes mellitus is still unknown. The aim of this study was to measure serum adiponectin, leptin and resistin levels and to investigate their relationships with vitamin D and other clinical and laboratory parameters in patients with type 1 diabetes. MATERIAL AND METHODS: Fifty subjects with type 1 diabetes and 50 healthy age- and sex-matched subjects were selected from the Endocrinology Outpatient Clinic of Cairo University Pediatrics Hospital...
June 2017: Archives of Medical Science: AMS
https://www.readbyqxmd.com/read/28719014/bioavailability-and-tolerability-of-nebulised-dexamethasone-sodium-phosphate-in-adult-horses
#5
A D Haspel, S Giguère, K A Hart, L J Berghaus, J L Davis
BACKGROUND: Nebulisation of the injectable dexamethasone sodium phosphate (DSP) would offer an inexpensive way of delivering a potent corticosteroid directly to the lungs of horses with asthma. However, this approach would be advantageous only if systemic absorption is minimal and if the preservatives present in the formulation do not induce airway inflammation. OBJECTIVE: To investigate the bioavailability of nebulised DSP and to determine whether it induces airway inflammation or hypothalamic-pituitary-adrenal (HPA) axis suppression in healthy adult horses...
July 18, 2017: Equine Veterinary Journal
https://www.readbyqxmd.com/read/28712183/association-of-abo-and-rh-blood-group-types-to-hepatitis-b-hepatitis-c-hiv-and-syphilis-infection-a-five-year-experience-in-healthy-blood-donors-in-a-tertiary-care-hospital
#6
Zufishan Batool, Saadia Haroon Durrani, Shazia Tariq
BACKGROUND: Aim of the study: The aim of the study was to find out the frequency of Hepatitis B Hepatitis C, Syphilis, HIV and malaria in apparently healthy blood donors and to find out any association between ABO and Rh blood groups. METHODS: It was a descriptive study carried out at Rehman Medical Institute laboratory. All blood donors who volunteered for blood donation from Jan 2008 to Dec 2014 were reviewed for blood groups and screening tests. Those who were eligible were then screened for Hepatitis B, Hepatitis C, HIV, syphilis and malaria on Architect 8200i through chemiluminescent immunoassay whereas malaria was screened by a thin film...
January 2017: Journal of Ayub Medical College, Abbottabad: JAMC
https://www.readbyqxmd.com/read/28708188/validation-of-a-membrane-touch-biosensor-for-the-qualitative-detection-of-igg-class-antibodies-to-herpes-simplex-virus-type-2
#7
Tony Loughman, Baljit Singh, Brian Seddon, Philip Noone, Padmanabhan Santhosh
A novel type of biosensor was assessed for application to the qualitative determination of circulating antibodies to herpes simplex virus type 2 (HSV-2). The device utilises a high activity HSV-2 type specific gG2 antigen for antibody capture and commercially available ELISA reagents. The study compares the diagnostic performance of a prototype HSV-2 biochip to well-established in vitro tests routinely applied in clinical procedures. A panel of human serum samples (n = 60) previously characterised for HSV-2 serological status using the DiaSorin LIAISON® HSV-2 chemiluminescent immunoassay were assayed on the HSV-2 biochip and the Focus Diagnostics HerpeSelect® 2 ELISA IgG kit to determine concordance with the predicate test method...
July 14, 2017: Analyst
https://www.readbyqxmd.com/read/28704828/extremely-high-prevalence-of-maternal-and-neonatal-vitamin-d-deficiency-in-the-arab-population
#8
Mona A Fouda, Iqbal Z Turkestani, Sara Almusharraf, Abdulrahman Al-Ajlan, Fatima F Angkaya-Bagayawa, Shaun Sabico, Ashry Gad Mohammed, Rana Hassanato, Amal Al-Serehi, Naemah M Alshingetti, Nasser M Al-Daghri
BACKGROUND: Vitamin D deficiency is a global public health problem. The published literature on vitamin D deficiency is limited among Arab pregnant women and its association with different metabolic markers. OBJECTIVE: To determine the prevalence of vitamin D deficiency in Arab pregnant women and neonates and its association with various biomarker profiles. METHODS: This is a multicenter study taken from a large prospective project in Riyadh, the capital city of Saudi Arabia...
July 14, 2017: Neonatology
https://www.readbyqxmd.com/read/28704441/a-comparison-of-sex-steroid-concentration-levels-in-the-vitreous-and-serum-of-patients-with-vitreoretinal-diseases
#9
Yuko Nishikawa, Seita Morishita, Taeko Horie, Masanori Fukumoto, Takaki Sato, Teruyo Kida, Hidehiro Oku, Jun Sugasawa, Tsunehiko Ikeda, Kimitoshi Nakamura
The purpose of this study was to compare steroid hormone concentration levels in the vitreous and serum of vitreoretinal disease patients to elucidate the possibility of neurosteroid production in the retina. Serum and vitreous samples were collected from vitrectomy patients, and estradiol (E2) and testosterone (T) concentrations were measured using electro-chemiluminescence immunoassay. We measured E2 in epiretinal membrane (ERM, n = 14), macular hole (MH, n = 18), proliferative diabetic retinopathy (PDR, n = 20), and retinal detachment (RD, n = 19) cases, and T in ERM (n = 14), MH (n = 17), PDR (n = 13), and RD (n = 17) cases...
2017: PloS One
https://www.readbyqxmd.com/read/28704179/analytical-evaluation-of-the-new-beckman-coulter-access-high-sensitivity-cardiac-troponin-i-immunoassay
#10
Giuseppe Lippi, Anna Ferrari, Giorgio Gandini, Matteo Gelati, Claudia Lo Cascio, Gian Luca Salvagno
BACKGROUND: This study was aimed to evaluate the analytical performance of the novel chemiluminescent and fully-automated Beckman Coulter Access hsTnI high-sensitivity immunoassay for measurement of cardiac troponin I (cTnI). METHODS: The study, using lithium heparin samples, included assessment of limit of blank (LOB), limit of detection (LOD), functional sensitivity, linearity, imprecision (within run, between-run and total), calculation of 99th percentile upper reference limit (URL) in 175 healthy blood donors (mean age, 36±12 years; 47% women) and comparison with two other commercial cTnI immunoassays...
July 12, 2017: Clinical Chemistry and Laboratory Medicine: CCLM
https://www.readbyqxmd.com/read/28702774/do-fsh-lh-ratio-and-gonadal-hormone-levels-predict-clinical-improvement-in-postmenopausal-schizophrenia-women
#11
Alexandre González-Rodríguez, Miquel Bernardo, Rafael Penadés, Bárbara Arias, Victoria Ruiz Cortés, Mary V Seeman, Rosa Catalán
Menopause is a process characterized by a decline in estrogen levels and is therefore a period of biological vulnerability for psychotic relapse in women with schizophrenia. Our goal was to correlate not only gonadal hormone levels but also follicle-stimulating hormone (FSH) and luteinizing hormone (LH) levels with improvement in specific clinical symptoms. Thirty-seven acutely ill postmenopausal schizophrenia women with a newly initiated, clinically determined change in antipsychotic medication participated in a 12-week prospective observational outcome study...
July 12, 2017: Archives of Women's Mental Health
https://www.readbyqxmd.com/read/28693499/conditions-of-gestation-childbirth-and-childhood-associated-with-c-peptide-in-young-adults-in-the-1982-birth-cohort-in-pelotas-rs-brazil
#12
Romildo Luiz Monteiro Andrade, Denise Petrucci Gigante, Isabel Oliveira de Oliveira, Bernardo Lessa Horta
BACKGROUND: The connecting peptide in insulin has been associated with cardiovascular risk and overall mortality in the adult population. However, its early determinants are unknown. Assess the association of exposures during pregnancy, delivery, and childhood with C-peptide among 22-23 years old individuals prospectively followed since birth, in a southern Brazilian city. METHODS: In 1982, all hospital births in the city were identified and those livebirths whose families lived in the urban area were evaluated (n = 5914)...
July 11, 2017: BMC Cardiovascular Disorders
https://www.readbyqxmd.com/read/28692637/comparison-of-2-human-chorionic-gonadotropin-immunoassays-commercially-available-for-monitoring-patients-with-gestational-trophoblastic-disease
#13
Juliana Maria Quinalha de Souza, Antonio Braga, Rafael Sanches Dos Santos, Marcos Montanha Ramos, Rafael Cortés-Charry, Izildinha Maestá
OBJECTIVE: The aim of this study was to compare serum human chorionic gonadotropin (hCG) levels in patients with gestational trophoblastic disease (GTD) using 2 commercially available hCG immunoassays. METHODS: Serum samples were obtained from patients with GTD attending the Botucatu Medical School Trophoblastic Diseases Center of São Paulo State University (UNESP), from November 2014 to October 2015. Serum hCG levels were measured with both Architect i2000SR and Immulite 2000 XPi chemiluminescence assays...
July 7, 2017: International Journal of Gynecological Cancer
https://www.readbyqxmd.com/read/28684903/serum-levels-of-igfbp7-are-elevated-during-acute-exacerbation-in-copd-patients
#14
Wenjing Ruan, Minliang Wu, Liuhong Shi, Fengying Li, Liangliang Dong, Yuanhua Qiu, Xiaohong Wu, Kejing Ying
OBJECTIVE: The purpose of this study was to explore the insulin-like growth factor binding protein 7 (IGFBP7) level in the serum of chronic obstructive pulmonary disease (COPD) patients during acute exacerbation (AE). METHODS: The study population consisted of 47 AECOPD patients, including 25 patients enrolled between January 2011 and February 2011 (the first group) and 22 patients enrolled from December 2011 to August 2012 (the second group) and 29 healthy controls...
2017: International Journal of Chronic Obstructive Pulmonary Disease
https://www.readbyqxmd.com/read/28682926/evaluation-of-the-novel-methotrexate-architect-chemiluminescent-inmunoassay-clinical-impact-on-pharmacokinetic-monitoring
#15
María Dolores Aumente, Julia López-Santamaría, Concepción Donoso-Rengifo, Inmaculada Reyes-Torres, Pablo Montejano Hervás
BACKGROUND: Fluorescence polarization immunoassay (FPIA) has been probably the most widely used technique for the determination of methotrexate (MTX) concentrations in clinical laboratories. After its replacement by a novel Architect chemiluminescent microparticle immunoassay (CMIA), it is essential to verify that there are no differences between the methods that can induce an error in leucovorin rescue with dire consequences for the patient. The objective of our study was to compare plasma/serum MTX measurements between CMIA and FPIA, reference method in this study, in the work conditions of a clinical pharmacokinetics unit to determine whether any difference would affect clinical decisions on the management of this drug...
July 3, 2017: Therapeutic Drug Monitoring
https://www.readbyqxmd.com/read/28680338/evaluation-of-selected-immunological-parameters-and-the-concentration-of-vitamin-d-in-children-with-asthma-case-control-study
#16
Agata Wawrzyniak, Agnieszka Lipińska-Opałka, Robert Zdanowski, Sławomir Lewicki, Piotr Murawski, Bolesław Kalicki
Due to the increased incidence of allergic diseases and emerging effects of unsatisfactory control of asthma, new mechanisms for supervising the immune system should be searched. The aim of the study was to analyze the percentage of CD3, CD4, CD8, CD19, CD16/56, NKT, CD3 anti-HLADR3 and Foxp3 regulatory lymphocytes in patients with asthma. Additionally the correlation between immune parameters, severity of asthma and serum concentration of vitamin D was performed. 25 children diagnosed with asthma were enrolled...
2017: Central-European Journal of Immunology
https://www.readbyqxmd.com/read/28680336/significant-reduction-of-peripheral-blood-interleukin-35-and-cd4-ebi3-t-cells-which-are-negatively-correlated-with-an-increase-in-the-plasma-il-17-and-ctni-level-in-viral-myocarditis-patients
#17
Han Ouyang, Li Xiang, Jianchang Chen, Weiting Xu, Yang Jiao, Huaying Shen
INTRODUCTION: Viral myocarditis (VMC) has become an increasingly common heart disease that endangers human health. In the present study, the plasma interleukin-35 (IL-35) level and the percentage of CD4(+)EBI3(+) T cells in VMC patients were detected to investigate the significance of changes in these parameters in the plasma of VMC patients and their association with the disease. MATERIAL AND METHODS: ELISA was performed to detect the plasma IL-35 level and the percentage of peripheral blood CD4(+)EBI3(+) T cells in 40 VMC patients and in 20 healthy individuals...
2017: Central-European Journal of Immunology
https://www.readbyqxmd.com/read/28679720/immunoassays
#18
Thomas O Kohl, Carl A Ascoli
The enzyme immunoassay (EIA) is one of the most powerful of all immunochemical techniques. First described in the early 1970s, these assays are now used routinely in laboratory analyses and diagnostics. In biology and biotechnology, the EIA is a valuable and versatile tool used to detect and quantitate antigens and antibodies. Application of the appropriate EIA permits rapid quantification of different antigens and antibodies (referred to here as analytes) present at very low concentrations within a mixture...
July 5, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28674718/seroprevalence-of-toxoplasma-gondii-rubella-virus-and-cytomegalovirus-among-pregnant-women-and-the-importance-of-avidity-assays
#19
Mumtaz C Sirin, Neval Agus, Nisel Yilmaz, Arzu Bayram, Yeser K Derici, Pinar Samlioglu, Sevgi Y Hanci, Guliz Dogan
OBJECTIVES: To determine the seroprevalence of Toxoplasma gondii (T. gondii), Rubella virus, and Cytomegalovirus (CMV) among pregnant women in Izmir, Turkey. METHODS: Medical records of pregnant women attending Izmir Tepecik Training and Research Hospital, Izmir, Turkey between January 2014 and January 2016 were analyzed in this retrospective cross-sectional study. The 7513 T. gondii IgM/IgG results, 7189 Rubella IgM/IgG results, 906 CMV IgM/IgG results and 146 avidity test results were evaluated...
July 2017: Saudi Medical Journal
https://www.readbyqxmd.com/read/28661055/evaluation-the-architect-ebv-vca-igm-vca-igg-and-ebna-1-igg-chemiluminescent-immunoassays-to-assess-ebv-serostatus-prior-transplantation
#20
Sarah Maylin, Linda Feghoul, Maud Salmona, Adel Herda, Séverine Mercier-Delarue, François Simon, Jérôme Legoff
Prior to transplantation of hematopoietic stem cells or solid organ, donor and recipient EBV serostatus has to be determined to assess risks of post-transplant lymphoproliferative disorders. Sensitivity of EBV Viral capsid antigens (VCA) IgG and EBV nuclear antigen-1 (EBNA-1) is critical to define past infection and a good specificity of VCA IgM is required to avoid any disqualification of cord blood (CB) units. Architect™ EBV antibody panel (Architect assay) providing a high throughput was compared to a semi-automated ELISA (Etimax assays Diasorin) to assess sensitivities and specificities of VCA and EBNA-1 IgG and VCA IgM on 419 sera collected from immunocompromised patients (n=184) and from pregnant women who agreed to give CB cells (n=235)...
June 29, 2017: Journal of Medical Virology
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