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chemiluminescence immunoassay

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https://www.readbyqxmd.com/read/28224431/clinical-value-of-serum-anti-mullerian-hormone-and-inhibin-b-in-prediction-of-ovarian-response-in-patients-with-polycystic-ovary-syndrome
#1
Fan Zhang, Xiao-Ling Liu, Nan Rong, Xiao-Wen Huang
The present study aimed to investigate the clinical value of serum anti-mullerian hormone (AMH) and inhibin B (INHB) in predicting the ovarian response of patients with polycystic ovary syndrome (PCOS). A total of 120 PCOS patients were enrolled and divided into three groups in terms of the ovarian response: a low-response group (n=36), a normal-response group (n=44), and a high-response group (n=40). The serum AMH and INHB levels were measured by enzyme-linked immunosorbent assay (ELISA). The follicle stimulating hormone (FSH), luteinizing hormone (LH), and estradiol (E2) levels were determined by chemiluminescence microparticle immunoassay...
February 2017: Journal of Huazhong University of Science and Technology. Medical Sciences
https://www.readbyqxmd.com/read/28224267/selection-of-dna-aptamers-for-extra-cellular-domain-of-human-epidermal-growth-factor-receptor-2-to-detect-her2-positive-carcinomas
#2
A Sett, B B Borthakur, U Bora
BACKGROUND: Human epidermal growth factor receptor 2 (Her2, an orphan receptor of ErbB family) is considered as an important biomarker as it plays a key role in the development and progression of aggressive types of breast, ovarian, stomach and gastric cancer. In the present study, we developed novel DNA aptamers against the extra-cellular domain (ECD) of Her2 protein for detection of Her2-positive carcinomas. METHODS: We cloned and expressed Her2-ECD protein in E...
February 21, 2017: Clinical & Translational Oncology
https://www.readbyqxmd.com/read/28224243/simultaneous-quantitation-of-endogenous-estrone-17%C3%AE-estradiol-and-estriol-in-human-serum-by-isotope-dilution-liquid-chromatography-tandem-mass-spectrometry-for-clinical-laboratory-applications
#3
Qiaoxuan Zhang, Liqiao Han, Jianbing Wang, Haibiao Lin, Peifeng Ke, Junhua Zhuang, Xianzhang Huang
Estrogen measurements are important in the assessment of female reproductive function and have expanding roles in other fields. A simple, accurate, highly sensitive and specific isotope-dilution liquid chromatography-tandem mass spectrometry method was developed and evaluated to simultaneously measure three endogenous estrogens in serum: estrone (E1), 17β-estradiol (E2), and estriol (E3). Chromatographic separation was achieved on a C18 column before electrospray ionization triple-quadrupole mass spectrometry in multiple reaction monitoring mode...
February 21, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28219358/mst-4-and-traf-6-expression-in-the-peripheral-blood-mononuclear-cells-of-patients-with-graves-disease-and-its-significance
#4
Ai Guo, Yan Tan, Chun Liu, Xiaoya Zheng
BACKGROUND: MST-4 and TRAF-6 are involved in the regulation of inflammatory and immune responses. However, whether they participate in the pathogenesis of Graves' disease (GD) has not yet been reported. Therefore, the purpose of this study was to investigate the expression of MST-4 and TRAF-6 in the peripheral blood of patients with GD to understand their role in the pathogenesis of GD. METHODS: Thirty newly diagnosed GD patients, 24 GD patients in remission (eGD) and 30 normal controls (NC) were recruited...
February 20, 2017: BMC Endocrine Disorders
https://www.readbyqxmd.com/read/28219030/in-situ-synthesized-cadmium-sulfide-nanowire-photosensor-with-a-parylene-passivation-layer-for-chemiluminescent-immunoassays
#5
Ju-Hee Im, Hong-Rae Kim, Byoung-Gi An, Young Wook Chang, Min-Jung Kang, Tae-Geol Lee, Jin Gyeng Son, Jae-Gwan Park, Jae-Chul Pyun
The direct in situ synthesis of cadmium sulfide (CdS) nanowires (NWs) was presented by direct synthesis of CdS NWs on the gold surface of an interdigitated electrode (IDE). In this work, we investigated the effect of a strong oxidant on the surfaces of the CdS NWs using X-ray photoelectron spectroscopy, transmission electron microscopy, and time-of-flight secondary ion mass spectrometry. We also fabricated a parylene-C film as a surface passivation layer for in situ-synthesized CdS NW photosensors and investigated the influence of the parylene-C passivation layer on the photoresponse during the coating of parylene-C under vacuum using a quartz crystal microbalance and a photoanalyzer...
February 16, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28214674/development-of-a-quick-serum-il-6-measuring-system-in-rheumatoid-arthritis
#6
Kensuke Koyama, Tetsuro Ohba, Kentaro Ishii, Giman Jung, Hirotaka Haro, Kenichi Matsuda
OBJECTIVE: Interleukin-6 (IL-6) plays a crucial role in the pathogenesis of rheumatoid arthritis (RA). Both fulfillment of remission criteria and assessment of other methods of evaluation of RA are important for preventing joint damage progression. Measurement of serum IL-6 concentrations has been reported to be useful for monitoring RA disease activity. However, it takes at least 4-5h to measure serum IL-6 concentrations using traditional methods, which limits its utility during routine assessment in daily clinical practice settings...
February 16, 2017: Cytokine
https://www.readbyqxmd.com/read/28207414/anti-aging-effects-of-m2000-%C3%AE-d-mannuronic-acid-as-a-novel-immunosuppressive-drug-on-the-enzymatic-and-non-enzymatic-oxidative-stress-parameters-in-an-experimental-model
#7
Soma Hosseini, Mohammad Abdollahi, Gholamreza Azizi, Mohammad Javad Fattahi, Noshin Rastkari, Farzaneh Tofighi Zavareh, Zahra Aghazadeh, Abbas Mirshafiey
BACKGROUND: The anti-aging property of β-D-mannuronic acid (M2000) as a novel non-steroidal anti-inflammatory and immunosuppressive agent was investigated on several determinants relative to the oxidative stress in an animal model. MATERIALS AND METHODS: Sprague-Dawley rats were used for evaluating the safety and efficacy properties of M2000 on some oxidative stress enzymes, including the following: mitochondrial superoxide dismutase (SOD2), catalase (CAT), glutathione peroxidase (GPX1), glutathione S-transferase (GST), myeloperoxidase (MPO), and inducible nitric oxide synthase (iNOS) gene expression by real-time PCR...
February 16, 2017: Journal of Basic and Clinical Physiology and Pharmacology
https://www.readbyqxmd.com/read/28205314/comparison-of-lc-ms-ms-vs-chemiluminescent-microparticle-immunoassay-in-measuring-the-valproic-acid-concentration-in-plasma-of-epilepsy-patients-in-a-new-perspective
#8
Zhipeng Wang, Yunlei Yun, Xinfang Xie, Chunhua You, Haijun Miao, Feng Zhang, Shouhong Gao, Wansheng Chen
OBJECTIVES: This study was designed to compare the performance of LC-MS/MS with chemiluminescent microparticle immunoassay (CMIA) for determination of VPA in epilepsy patients in the perspective of metabolites' hepatotoxicity. METHOD: Samples were collected and then analyzed using both LC-MS/MS and CMIA. A LO2 cells (normal human hepatic cells) experiment was carried out to confirm VPA metabolites' hepatotoxicity using AST(Aspertate Aminotransferase, AST), ALT(Alanine aminotransferase, ALT) and LDH(lactate dehydrogenase, LDH) in supernate as index...
February 15, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/28202792/towards-a-new-strategy-for-the-diagnosis-of-congenital-trypanosoma-cruzi-infection
#9
Alba Abras, Carmen Muñoz, Cristina Ballart, Pere Berenguer, Teresa Llovet, Mercedes Herrero, Silvia Tebar, María-Jesús Pinazo, Elizabeth Posada, Carmen Martí, Victoria Fumadó, Jordi Bosch, Oriol Coll, Teresa Juncosa, Gemma Ginovart, Josep Armengol, Joaquim Gascón, Montserrat Portús, Montserrat Gállego
The immigration of Latin American women of childbearing age has spread the congenital transmission of Chagas disease to non-endemic areas, and the disease is now a worldwide problem. Some European health authorities have implemented screening programs to prevent vertical transmission, but the lack of a uniform protocol calls for the urgent establishment of a new strategy, common for all laboratories. Our aims were (i) to analyze the trend of passive IgG antibodies in the newborn by means of five serological tests for the diagnosis and follow-up of congenital Trypanosoma cruzi infection, (ii) to assess the utility of these techniques for diagnosing a congenital transmission, and (iii) to propose a strategy for a prompt, efficient and cost-effective diagnosis of T...
February 15, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28193202/association-of-high-serum-vitamin-d-concentrations-with-active-pulmonary-tb-in-an-hiv-co-endemic-setting-harare-zimbabwe
#10
Cuthbert Musarurwa, Lynn Sodai Zijenah, Doreen Zvipo Duri, Kudzie Mateveke-Dangaiso, Kudakwashe Mhandire, Maria Mary Chipiti, Marshall Wesley Munjoma, Witmore Bayayi Mujaji
BACKGROUND: There is paucity data on the association of vitamin D deficiency (VDD) and active tuberculosis (TB) in southern Africa where the human immunodeficiency virus (HIV) is co-endemic. We examined the association of serum vitamin D concentrations with active pulmonary tuberculosis (PTB) in HIV-infected (n = 284) and uninfected (n = 267) Black Zimbabweans, in Harare, Zimbabwe. METHODS: We conducted a cross-sectional study of 551 participants comprising 145 HIV(+)/PTB (+), 139 HIV(+)/PTB(-), 134 HIV(-)/PTB(+) and 133 HIV(-)/PTB(-)...
February 13, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28192156/evaluation-of-two-novel-chemiluminescence-immunoassays-for-the-detection-of-clostridium-difficile-glutamate-dehydrogenase-and-toxin-a-b
#11
Annette Blaich, Reno Frei, Carine Castellano, Christine Kiessling, Angelika Geschke, Katharina M Rentsch, Adrian Egli
A novel immunoassay for Clostridium difficile glutamate dehydrogenase (GDH) and toxin A&B (LIAISON, DiaSorin) was compared to another GDH assay (Alere), PCR and toxigenic culture. The GDH-DiaSorin is slightly more sensitive than the GDH-Alere. Sensitivity of the Toxin-Diasorin test is in accordance to the sensitivity of other immunoassays in literature.
February 10, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28187244/analytical-performance-of-three-diagnostic-reagents-for-hbsag-on-an-automatic-elisa-analyzer
#12
Jiayi Hou, Jianping Ren, Ling Song, Fen Zhao, Peijun Liang
PURPOSE: We conducted performance tests of three HBsAg ELISA diagnostic reagents using an Addcare 600 (Yantai Addcare Bio-tech Limited Company) and studied the consistency between the qualitative results and chemiluminescent microparticle immunoassay (CMIA) results. METHODS: Diagnostic kits (ELISA) for HBsAg manufactured by INTEC ("A"), KHB ("B") and Wantai ("C") were tested on an Addcare 600 to evaluate their intermediate precision, repeatability, and C50. Furthermore, three ELISA detection systems and a quantitative test kit for HBsAg (Abbott) were employed to screen 1000 serum samples, while CMIA reactive samples were used to perform the confirmatory tests...
February 10, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/28182361/evaluation-of-line-immunoassay-to-detect-htlv-1-infection-in-an-endemic-area-southwestern-japan-comparison-with-polymerase-chain-reaction-and-western-blot
#13
Kazumi Umeki, Kunihiko Umekita, Yuuki Hashikura, Ikuo Yamamoto, Kazuyoshi Kubo, Yasuhiro Nagatomo, Akihiko Okayama
BACKGROUND: Human T-lymphotropic virus type 1 (HTLV-1) has been recognized as a cause of adult T-cell leukemia/lymphoma, HTLV-1-associated myelopathy/tropical spastic paraparesis, and HTLV-1-associated uveitis. HTLV-1 infection is normally detected by screening for HTLV-1 antibodies, and positive samples are confirmed by Western blot (WB). However, WB fails to confirm some samples that were positive for HTLV-1 antibodies on screening. Line immunoassay (LIA) is commonly used in Europe and Brazil, but not in Japan...
February 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/28181032/comprehensive-evaluation-of-chemiluminescent-immunoassays-for-the-laboratory-diagnosis-of-clostridium-difficile-infection
#14
A Makristathis, I Zeller, D Mitteregger, M Kundi, A M Hirschl
For the microbiological diagnosis of a Clostridium (C.) difficile infection (CDI), a two-test algorithm consisting of a C. difficile glutamate dehydrogenase (GDH)-immunoassay followed by a toxin-immunoassay in positive cases is widely used. In this study, two chemiluminescent immunoassays (CLIAs), one for GDH and the other for the toxins A and B, have been evaluated systematically using appropriate reference methods. Three-hundred diarrhoeal stool specimens submitted for CDI diagnosis were analysed by the LIAISON CLIAs (DiaSorin)...
February 8, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28171686/the-relationship-between-procalcitonin-and-thyroid-autoantibodies-in-patients-with-autoimmune-thyroiditis
#15
Ali Oncul, Ihsan Ates, Mehmet Fettah Arikan, Nisbet Yilmaz, Canan Topcuoglu, Fatma Meric Yilmaz, Mustafa Altay
BACKGROUND: The aim of this study is to investigate the serum levels of procalcitonin and its association with autoantibodies in patients with euthyroid Hashimoto's thyroiditis. METHODS: A total of 80 participants were included in the study; 40 of which were newly diagnosed with Hashimoto's thyroiditis, aged over 18, and 40 of which were healthy volunteers. The serum levels of procalcitonin were measured by enzyme-linked immunosorbent assay kit. Thyroid function tests were analyzed in hormone laboratory with Electro-chemiluminescence immunoassay...
February 7, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/28169461/harmonization-of-serum-25-hydroxycalciferol-assay-results-from-high-performance-liquid-chromatography-enzyme-immunoassay-radioimmunoassay-and-immunochemiluminescence-systems-a-multicenter-study
#16
Bahareh Nikooyeh, Siamak M Samiee, Marjan R Farzami, Hamid Alavimajd, Maliheh Zahedirad, Ali Kalayi, Nastaran Shariatzadeh, Nasrin Boroumand, Elham Golshekan, Yalda Gholamian, Tirang R Neyestani
BACKGROUND: Remarkable disagreement among different systems of 25-hydroxy vitamin D 25(OH)D assay makes decision making for both clinical and community interventions very difficult. This study aimed to harmonize the results obtained from different 25(OH)D assay systems. METHODS: A total of 275 serum samples were analyzed for 25(OH)D using DIAsource-enzyme immunoassay (EIA), DIAsource-radioimmunoassay (RIA), Roche-electrochemiluminescence (ECL), Diasorin-chemiluminescent immunoassay (CLIA), and high-performance liquid chromatography (HPLC), as the reference method...
February 7, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/28164642/serum-homocysteine-folate-and-vitamin-b12-levels-in-carbamazepine-treated-epileptic-children
#17
Tarun K Sharma, S K Vardey, S Sitaraman
BACKGROUND: Antiepileptic drugs (AEDs) have been associated with metabolic consequences that lead to an increase in risk of atherosclerosis in patients with epilepsy. Therefore, we evaluated whether differences exist in homocysteine, folate, and vitamin B12 levels in children receiving carbamazepine (CBZ) monotherapy. METHODS: A total of 58 newly diagnosed epileptic children with ages ranging from 2 to 15 years were enrolled at the start of study. However, after 3 months follow up, the final total sample size was only 50 epileptic children...
July 1, 2016: Clinical Laboratory
https://www.readbyqxmd.com/read/28164508/urinary-neutrophil-gelatinase-associated-lipocalin-does-not-distinguish-acute-rejection-from-other-causes-of-acute-kidney-injury-in-pediatric-renal-transplant-recipients
#18
Tomas Seeman, Karel Vondrak, Jiri Dusek, Nadezda Simankova, Jakub Zieg, Jaromir Hacek, Maria Chadimova, Bruno Sopko, Magdalena Fortova
BACKGROUND: The aim of this prospective single center study was to investigate the ability of urinary neutrophil gelatinase-associated lipocalin (NGAL) to distinguish acute rejection from other causes of acute kidney injury (AKI) in children after renal transplantation. METHODS: Fifteen children fulfilled the inclusion criteria (acute kidney injury (AKI) with allograft biopsy, at least 21 days after renal transplantation, no sepsis) during 2013 - 2014 in our pediatric transplantation center...
January 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/28155925/an-enzyme-induced-au-ag-core-shell-nanostructure-used-for-an-ultrasensitive-surface-enhanced-raman-scattering-immunoassay-of-cancer-biomarkers
#19
Lin Yang, Ming Xuan Gao, Lei Zhan, Min Gong, Shu Jun Zhen, Cheng Zhi Huang
Early detection of cancer is helpful for the control and prevention of diseases. Due to the low content of cancer biomarkers in the early disease phases, however, an ultrasensitive and selective method is critical. In this contribution, an ultrasensitive surface-enhanced Raman scattering (SERS) immunoassay is newly developed with the principle of introducing a common enzyme-induced deposition (EID) reaction to coat a silver layer on the surface of gold nanoparticles and to form a core-shell nanostructure of Au@Ag...
February 3, 2017: Nanoscale
https://www.readbyqxmd.com/read/28153228/a-novel-microchip-electrophoresis-based-chemiluminescence-immunoassay-for-the-detection-of-alpha-fetoprotein-in-human-serum
#20
Jingwen Liu, Jingjin Zhao, Shuting Li, Liangliang Zhang, Yong Huang, Shulin Zhao
A sensitive immunoassay method based on microchip electrophoresis chemiluminescence (MCE-CL) detection technology was developed for the detection of tumor marker alpha-fetoprotein (AFP). This method adopts the non-competitive immunoassay mode, and was conducted after AFP reacted with excessive horseradish peroxidase (HRP) labeled monoclonal antibody. The extreme pH value was adopted in the electrophoresis buffer solution. The use of brij 35 as an additive of electrophoresis buffer increased dramatically the resolution (Rs) and the reproducibility of the analysis...
April 1, 2017: Talanta
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