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chemiluminescence immunoassay

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https://www.readbyqxmd.com/read/28329932/-an-investigation-on-immunological-effect-of-hepatitis-b-vaccine-amongst-adult-population-in-high-labor-export-rural-regions-under-4-different-strategies
#1
X Y Zheng, Z H Ji, Z W Guo, Y W Liu, Z J Shao, Y P Yan
Objective: To grope for an ideal immune strategy in grown-ups via comparison of immunological effects under 4 different vaccination schemes. Methods: Study population was selected by stratified random cluster sampling. A total of 4 different vaccination proposals, including Strategy A (3 doses, 10 μg, administrated repeatedly into the unilateral deltoid muscle at 0-1-6 months), Strategy B (2 doses, 20 μg, administrated into the bilateral deltoid muscles simultaneously), Strategy C (3 doses, 10 μg, administrated repeatedly into the unilateral deltoid muscle at 0-1-2 months) and Strategy D (2 doses, 10 μg, administrated to the bilateral deltoid muscles at the same time), were conducted in Liangzhou, Minqin Gulang, and the Tianzhu Tibetan Autonomic county respectively, in Wuwei city, Gansu province...
March 10, 2017: Zhonghua Liu Xing Bing Xue za Zhi, Zhonghua Liuxingbingxue Zazhi
https://www.readbyqxmd.com/read/28329259/evaluation-of-modified-2-tiered-serodiagnostic-testing-algorithms-for-early-lyme-disease
#2
John A Branda, Klemen Strle, Lise E Nigrovic, Paul M Lantos, Timothy J Lepore, Nitin S Damle, Mary Jane Ferraro, Allen C Steere
Background.: The conventional 2-tiered serologic testing protocol for Lyme disease (LD), an enzyme immunoassay (EIA) followed by immunoglobulin M and immunoglobulin G Western blots, performs well in late-stage LD but is insensitive in patients with erythema migrans (EM), the most common manifestation of the illness. Western blots are also complex, difficult to interpret, and relatively expensive. In an effort to improve test performance and simplify testing in early LD, we evaluated several modified 2-tiered testing (MTTT) protocols, which use 2 assays designed as first-tier tests sequentially, without the need of Western blots...
January 27, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/28326299/the-association-between-dehydroepiandrosterone-sulfate-dhea-s-and-bone-mineral-density-in-korean-men-and-women
#3
Seung-Gun Park, Sena Hwang, Jong-Suk Kim, Kyung-Chae Park, Yuri Kwon, Kyong-Chol Kim
BACKGROUND: The relationship between dehydroepiandrosterone sulfate (DHEA-S) and bone mineral density (BMD) is controversial. And findings of most studies that have investigated this relationship are restricted to postmenopausal women. In this study, we investigated the relationship between serum DHEA-S and BMD in both men and women. METHODS: This cross-sectional study evaluated a total of 294 healthy Korean participants through a medical examination program. And a subgroup of 154 participants was subjected to a longitudinal analysis...
February 2017: Journal of Bone Metabolism
https://www.readbyqxmd.com/read/28321506/standardizing-25-hydroxyvitamin-d-data-from-the-hunmen-cohort
#4
E Jakab, E Kalina, Z Petho, Z Pap, A Balogh, W B Grant, H P Bhattoa
Standardization of 25-hydroxyvitamin D (25OHD) values is still a challenge. We propose standardization by correction of the measured 25OHD values using the linear regression bias from the National Institute of Standards and Technology (NIST) 'total' target values reported by Vitamin D External Quality Assessment Scheme (DEQAS). Our approach could perhaps be a practical solution to the anomaly surrounding non-standardized 25OHD values. INTRODUCTION: Standardization of 25OHD values is still a challenge...
March 20, 2017: Osteoporosis International
https://www.readbyqxmd.com/read/28302204/-a-preliminary-study-of-retinoblastoma-related-serum-tumor-markers
#5
Zhi-Ping Liu, Ke-Ying Zhou, Li-Li Chen, Zhi-Hui Xiao, Yan-Zhao Chen
OBJECTIVE: To screen out retinoblastoma (RB)-related serum tumor markers by measuring the levels of serum alpha fetoprotein (AFP), carcino-embryonic antigen (CEA), neuron-specific enolase (NSE), carbohydrate antigen 125 (CA125), carbohydrate antigen 153 (CA153), carbohydrate antigen 199 (CA199), and carbohydrate antigen 724 (CA724) in children with RB. METHODS: The levels of seven serum tumor markers (AFP, CEA, NSE, CA125, CA153, CA199, and CA724) were determined in 20 children with RB and 20 healthy children (control) using a chemiluminescent immunoassay...
March 2017: Zhongguo Dang Dai Er Ke za Zhi, Chinese Journal of Contemporary Pediatrics
https://www.readbyqxmd.com/read/28293250/expression-of-a-human-prostatic-acid-phosphatase-pap-igm-fc-fusion-protein-in-plants-using-in-vitro-tissue-subculture
#6
Yang J Kang, Deuk-Su Kim, Soon-Chul Myung, Kisung Ko
In this study, prostatic acid phosphatase (PAP), which is overexpressed in human prostate cancer cells, was cloned to be fused to the IgM constant fragment (Fc) for enhancing immunogenicity and expressed in transgenic tobacco plants. Then, the transgenic plants were propagated by in vitro tissue subculture. Gene insertion and expression of the recombinant PAP-IgM Fc fusion protein were confirmed in each tested the first, second, and third subculture generations (SG1, SG2, and SG3, respectively). Transcription levels were constantly maintained in the SG1, SG2, and SG3 leaf section (top, middle, and base)...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28288197/dog-cloning-with-in-vivo-matured-oocytes-obtained-using-electric-chemiluminescence-immunoassay-predicted-ovulation-method
#7
Seunghoon Lee, Minghui Zhao, Jingu No, Yoonseok Nam, Gi-Sun Im, Tai-Young Hur
Radioactive immunoassay (RIA) is a traditional serum hormone assay method, but the application of the method in reproductive studies is limited by the associated radioactivity. The aim of present study was to evaluate the reliability of RIA and to compare its canine serum progesterone concentration determination accuracy to that of the electric chemiluminescence immunoassay (ECLI). In vivo matured oocytes were utilized for canine somatic cell nuclear transfer (SCNT), and serum progesterone levels were assessed to accurately determine ovulation and oocyte maturation...
2017: PloS One
https://www.readbyqxmd.com/read/28279841/european-multicentre-pilot-survey-to-assess-vitamin-d-status-in-rheumatoid-arthritis-patients-and-early-development-of-a-new-patient-reported-outcome-questionnaire-d-pro
#8
REVIEW
Jelena Vojinovic, Angela Tincani, Alberto Sulli, Stefano Soldano, Laura Andreoli, Francesca Dall'Ara, Ruxandra Ionescu, Katarina Simic Pasalic, Inete Balcune, Ivan Ferraz-Amaro, Małgorzata Tlustochowicz, Irena Butrimiene, Egle Punceviciene, Natalia Toroptsova, Simeon Grazio, Jadranka Morovic-Vergles, Pavol Masaryk, Kati Otsa, Miguel Bernardes, Vladimira Boyadzhieva, Fausto Salaffi, Maurizio Cutolo
OBJECTIVE: To collect data on vitamin D (25(OH)D) serum levels in a large number of rheumatoid arthritis (RA) patients from different European countries, to investigate their relation with disease activity, disability, quality of life, and possibly to construct a new Patient Reported Outcome (PRO) questionnaire in order to self-estimate if they are at risk for vitamin D insufficiency/deficiency-related clinical implications (D-PRO). METHODS: This was a European League Against Rheumatism (EULAR) supported cross-sectional study (project No CLI064) which involved 625 RA patients (mean age 55±11 years, mean disease duration 11±9 years), 276 age and sex matched healthy subjects, and rheumatologists working in academic institutions or hospital centres, as well as PARE organizations (patient representatives) from 13 European countries...
March 6, 2017: Autoimmunity Reviews
https://www.readbyqxmd.com/read/28276194/a-highly-sensitive-detection-of-chloramphenicol-based-on-chemiluminescence-immunoassays-with-the-cheap-functionalized-fe3-o4-sio2-magnetic-nanoparticles
#9
Wang Linyu, Yao Manwen, Fang Chengzhi, Yao Xi
A strategy has been applied to chloramphenicol (CAP) detection with chemiluminescence immunoassays (CLIA) based on cheap functionalized Fe3 O4 @SiO2 magnetic nanoparticles (Fe-MNPs). The strategy that bovine serum albumin (BSA) was immobilized on cheap functionalized Fe-MNPs and that the CAP molecules were then immobilized on BSA, avoided the long process of dialysis for preparation of the BSA-CAP conjugates. The samples were detected for both methods that utilized two different kinds of functionalized Fe-MNPs (amine-functionalized Fe3 O4 @SiO2 and carboxylic acid-functionalized Fe3 O4 @SiO2 )...
March 8, 2017: Luminescence: the Journal of Biological and Chemical Luminescence
https://www.readbyqxmd.com/read/28271738/profile-of-instrumentation-laboratory-s-hemosil%C3%A2-acustar-hit-ab-pf4-h-assay-for-diagnosis-of-heparin-induced-thrombocytopenia
#10
Michael Nagler, Adam C Cuker
Immunoassays play an essential role in the diagnosis of heparin-induced thrombocytopenia (HIT). The objective of this article is to review HemosIL® AcuStar HIT-Ab(PF4-H) (Instrumentation Laboratory, Bedford, MA, USA), a new chemiluminescent immunoassay for HIT. Areas covered: The authors searched the published literature for evaluation studies of HemosIL® AcuStar HIT-Ab(PF4-H) and sought information from the manufacturer. In this paper, the authors discuss the analytical principle and technical aspects of the assay; describe its diagnostic performance in validation studies; report on its reproducibility, cost-effectiveness, and regulatory status; and discuss the implications of the assay on clinical practice and means of integrating it in diagnostic pathways...
March 8, 2017: Expert Review of Molecular Diagnostics
https://www.readbyqxmd.com/read/28271696/folate-deficiency-and-gene-polymorphisms-of-mthfr-mtr-and-mtrr-elevate-the-hyperhomocysteinemia-risk
#11
Wen-Xing Li, Fei Cheng, A-Jie Zhang, Shao-Xing Dai, Gong-Hua Li, Wen-Wen Lv, Tao Zhou, Qiang Zhang, Hong Zhang, Tao Zhang, Fang Liu, Dahai Liu, Jing-Fei Huang
BACKGROUND: Hyperhomocysteinemia (HHcy) is an independent risk factor for cardiovascular diseases (CVDs). We aimed to investigate the joint effect of homocysteine metabolism gene polymorphisms, as well as the folate deficiency on the risk of HHcy in a Chinese hypertensive population. METHODS: This study enrolled 480 hypertensive patients aged 28 - 75 from six hospitals in different Chinese regions from 9/2005 - 12/2005. Known genotypes of methylenetetrahydrofolate reductase (MTHFR) C677T and A1298C, methionine synthase (MTR) A2756G, and methionine synthase reductase (MTRR) A66G were detected by PCRRFLP methods...
March 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/28259362/a-systematic-review-of-methods-for-quantifying-serum-testosterone-in-patients-with-prostate-cancer-who-underwent-castration
#12
I Comas, R Ferrer, J Planas, A Celma, L Regis, J Morote
BACKGROUND: The clinical practice guidelines recommend measuring serum testosterone in patients with prostate cancer (PC) who undergo castration. The serum testosterone concentration should be <50ng/dL, a level established by using a radioimmunoassay method. The use of chemiluminescent immunoassays (IA) has become widespread, although their metrological characteristics do not seem appropriate for quantifying low testosterone concentrations. The objective of this review is to analyse the methods for quantifying testosterone and to establish whether there is scientific evidence that justifies measuring it in patients with PC who undergo castration, through liquid chromatography attached to a mass spectrometry in tandem (LC-MSMS)...
March 2, 2017: Actas Urologicas Españolas
https://www.readbyqxmd.com/read/28250769/pediatric-tsh-reference-intervals-and-prevalence-of-high-thyroid-antibodies-in-the-lebanese-population
#13
Marie-Hélène Gannagé-Yared, Nicole Balech, Vanessa Farah, Marianne Antar, Rindala Saliba, Elise Chahine
The aims of this study are to establish reference values for TSH in Lebanese schoolchildren; to look at the relationship between TSH and age, gender, BMI, socioeconomic status (SES), and thyroid antibodies (TAb); and to investigate the prevalence of abnormal TAb in this population. 974 Lebanese schoolchildren aged 8-18 years were recruited from 10 schools of different SES. Third-generation TSH, TPO-Ab, and Tg-Ab measurements were performed using the IMMULITE chemiluminescent immunoassay. The mean TSH is 2.06 ± 1...
2017: International Journal of Endocrinology
https://www.readbyqxmd.com/read/28248852/serosurveillance-of-hepatitis-a-in-a-region-which-adopted-the-universal-mass-vaccination
#14
Maria Filomena Gallone, Francesco Desiante, Maria Serena Gallone, Giovanna Barbuti, Silvio Tafuri, Cinzia Germinario
Hepatitis A is a common infectious disease worldwide that was endemic in many regions of Southern Italy, such as Apulia region. After a large hepatitis A outbreak occurred between 1996 and 1997, in Apulia an active-free immunization program that was targeted to new-borns and adolescents was started. The aim of this study is to investigate the hepatitis A seroprevalence in the adult Apulian population 18 years after the immunization program introduction, in order to evaluate the risk of new epidemics onset.The study was carried out from May 2011 to June 2012 among blood donors from Department of Transfusion Medicine and Blood Bank of Policlinico General Hospital in Bari...
March 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28242262/vitamin-d-assays-in-clinical-laboratory-past-present-and-future-challenges
#15
Shereen H Atef
Vitamin D status is usually assessed by measuring the serum 25-hydroxyvitamin D (25(OH)D) concentration. There has been a dramatic increase in 25-OHD requests over recent years prompting many laboratories to consider the use of automated immunoassays. In this presentation, we will discuss and compare the two major techniques that are used for measuring of vitamin D (the binding assay and chemical assay techniques). Chemiluminescence immunoassays (CLIA), radioimmunoassy (RIA), and binding protein assay are belonging to the binding assay, while the chemical assay includes high performance liquid chromatography (HPLC) and liquid chromatography-tandem mass spectrometry (LC-MS/MS)...
February 24, 2017: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/28230309/evaluation-of-positive-and-false-positive-results-in-syphilis-screening-of-blood-donors-in-rio-de-janeiro-brazil
#16
V S Sandes, S G C Silva, I J F Motta, L G C Velarde, S R de Castilho
OBJECTIVES: We propose to analyse the positive and false-positive results of treponemal and nontreponemal tests in blood donors from Brazil and to evaluate possible factors associated with the results of treponemal tests. BACKGROUND: Treponemal tests have been used widely for syphilis screening in blood banks. The introduction of these tests in donor screening has caused an impact and a loss of donors who need to be assessed. METHODS: This was a retrospective cross-sectional study of syphilis screening and confirmatory test results of blood donors that were obtained before and after adopting a chemiluminescent immunoassay (CLIA)...
February 23, 2017: Transfusion Medicine
https://www.readbyqxmd.com/read/28224431/clinical-value-of-serum-anti-mullerian-hormone-and-inhibin-b-in-prediction-of-ovarian-response-in-patients-with-polycystic-ovary-syndrome
#17
Fan Zhang, Xiao-Ling Liu, Nan Rong, Xiao-Wen Huang
The present study aimed to investigate the clinical value of serum anti-mullerian hormone (AMH) and inhibin B (INHB) in predicting the ovarian response of patients with polycystic ovary syndrome (PCOS). A total of 120 PCOS patients were enrolled and divided into three groups in terms of the ovarian response: a low-response group (n=36), a normal-response group (n=44), and a high-response group (n=40). The serum AMH and INHB levels were measured by enzyme-linked immunosorbent assay (ELISA). The follicle stimulating hormone (FSH), luteinizing hormone (LH), and estradiol (E2) levels were determined by chemiluminescence microparticle immunoassay...
February 2017: Journal of Huazhong University of Science and Technology. Medical Sciences
https://www.readbyqxmd.com/read/28224267/selection-of-dna-aptamers-for-extra-cellular-domain-of-human-epidermal-growth-factor-receptor-2-to-detect-her2-positive-carcinomas
#18
A Sett, B B Borthakur, U Bora
BACKGROUND: Human epidermal growth factor receptor 2 (Her2, an orphan receptor of ErbB family) is considered as an important biomarker as it plays a key role in the development and progression of aggressive types of breast, ovarian, stomach and gastric cancer. In the present study, we developed novel DNA aptamers against the extra-cellular domain (ECD) of Her2 protein for detection of Her2-positive carcinomas. METHODS: We cloned and expressed Her2-ECD protein in E...
February 21, 2017: Clinical & Translational Oncology
https://www.readbyqxmd.com/read/28224243/simultaneous-quantitation-of-endogenous-estrone-17%C3%AE-estradiol-and-estriol-in-human-serum-by-isotope-dilution-liquid-chromatography-tandem-mass-spectrometry-for-clinical-laboratory-applications
#19
Qiaoxuan Zhang, Liqiao Han, Jianbing Wang, Haibiao Lin, Peifeng Ke, Junhua Zhuang, Xianzhang Huang
Estrogen measurements are important in the assessment of female reproductive function and have expanding roles in other fields. A simple, accurate, highly sensitive and specific isotope-dilution liquid chromatography-tandem mass spectrometry method was developed and evaluated to simultaneously measure three endogenous estrogens in serum: estrone (E1), 17β-estradiol (E2), and estriol (E3). Chromatographic separation was achieved on a C18 column before electrospray ionization triple-quadrupole mass spectrometry in multiple reaction monitoring mode...
February 21, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28219358/mst-4-and-traf-6-expression-in-the-peripheral-blood-mononuclear-cells-of-patients-with-graves-disease-and-its-significance
#20
COMPARATIVE STUDY
Ai Guo, Yan Tan, Chun Liu, Xiaoya Zheng
BACKGROUND: MST-4 and TRAF-6 are involved in the regulation of inflammatory and immune responses. However, whether they participate in the pathogenesis of Graves' disease (GD) has not yet been reported. Therefore, the purpose of this study was to investigate the expression of MST-4 and TRAF-6 in the peripheral blood of patients with GD to understand their role in the pathogenesis of GD. METHODS: Thirty newly diagnosed GD patients, 24 GD patients in remission (eGD) and 30 normal controls (NC) were recruited...
February 20, 2017: BMC Endocrine Disorders
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