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chemiluminescence immunoassay

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https://www.readbyqxmd.com/read/29145466/serum-tsh-levels-as-a-predictor-of-malignancy-in-thyroid-nodules-a-prospective-study
#1
Lenara Golbert, Ana Patrícia de Cristo, Carlo Sasso Faccin, Mauricio Farenzena, Heloísa Folgierini, Marcia Silveira Graudenz, Ana Luiza Maia
BACKGROUND: The role of serum TSH concentrations as a predictor of malignancy of thyroid nodule remains unclear. OBJECTIVE: To prospectively evaluate the usefulness of serum TSH levels as a predictor of malignancy in thyroid nodules. METHODS: Patients with thyroid nodule(s) who underwent fine-needle aspiration biopsy under ultrasonographic guidance in a tertiary, university-based hospital were consecutively evaluated. Patients with known thyroid cancer and/or patients receiving thyroid medication were excluded...
2017: PloS One
https://www.readbyqxmd.com/read/29143619/diagnostic-accuracy-of-tests-to-detect-hepatitis-b-surface-antigen-a-systematic-review-of-the-literature-and-meta-analysis
#2
Ali Amini, Olivia Varsaneux, Helen Kelly, Weiming Tang, Wen Chen, Debrah I Boeras, Jane Falconer, Joseph D Tucker, Roger Chou, Azumi Ishizaki, Philippa Easterbrook, Rosanna W Peeling
BACKGROUND: Chronic Hepatitis B Virus (HBV) infection is characterised by the persistence of hepatitis B surface antigen (HBsAg). Expanding HBV diagnosis and treatment programmes into low resource settings will require high quality but inexpensive rapid diagnostic tests (RDTs) in addition to laboratory-based enzyme immunoassays (EIAs) to detect HBsAg. The purpose of this review is to assess the clinical accuracy of available diagnostic tests to detect HBsAg to inform recommendations on testing strategies in 2017 WHO hepatitis testing guidelines...
November 1, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/29140979/ginsenoside-rg3-inhibits-angiogenesis-in-a-rat-model-of-endometriosis-through-the-vegfr-2-mediated-pi3k-akt-mtor-signaling-pathway
#3
Yang Cao, Qing Ye, Mengfei Zhuang, Shuwu Xie, Ruihua Zhong, Jingang Cui, Jieyun Zhou, Yan Zhu, Tingting Zhang, Lin Cao
OBJECTIVE: This study aimed to investigate the link between the inhibitory effect of ginsenoside Rg3 on the ectopic endometrium growth and the VEGFR-2-mediated PI3K/Akt/mTOR signaling pathway, a mechanism known to inhibit angiogenesis and induce ectopic endometrial cell apoptosis. MATERIALS AND METHODS: A model of endometriosis was established by allotransplantation in rats. The rats were randomly divided into 5 groups: the ginsenoside Rg3 low-dose group (group A,5mg/kgBW/d of ginsenoside Rg3), the ginsenoside Rg3 high-dose group (group B, 10mg/kgBW/d of ginsenoside Rg3), the gestrinone group (group C, 0...
2017: PloS One
https://www.readbyqxmd.com/read/29137944/reproductive-factors-nutritional-status-and-serum-25-oh-d-levels-in-women-with-breast-cancer-a-case-control-study
#4
REVIEW
Nighat Y Sofi, Monika Jain, Umesh Kapil, Vuthaluru Seenu, Lakshmy R, Chander P Yadav, Ravindra M Pandey, Neha Sareen
The study was conducted with an objective to investigate the association between reproductive factors, nutritional status and serum 25(OH)D levels among women diagnosed with breast cancer (BC). A total of 200 women with BC attending a tertiary healthcare institute of Delhi, India matched with 200 healthy women for age (±2years) and socio economic status were included in the study. Data was collected on socio-demographic profile, reproductive factors, physical activity and dietary intake (24h dietary recall and food frequency questionnaire) using interviewer administered structured questionnaires and standard tools...
November 11, 2017: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/29132457/-relationship-between-serum-25-oh-d-levels-at-birth-and-respiratory-distress-syndrome-in-preterm-infants
#5
Ren-Qiang Yu, Dao-Zhen Chen, Xiao-Qing Hao, Shi-Hong Jiang, Guang-Dong Fang, Qin Zhou
OBJECTIVE: To investigate the relationship between serum 25-hydroxyvitamin D [25(OH)D] levels at birth and respiratory distress syndrome (RDS) in preterm infants. METHODS: This retrospective study recruited preterm infants with gestational age of below 34 weeks who were born between January 2014 and December 2016. These preterm infants were divided into two groups: RDS (n=72) and control (n=40). Clinical data of the two groups were collected, including gestational age, birth weight, gender, delivery mode, Apgar scores at 1 minute and 5 minutes, incidence of maternal gestational diabetes mellitus, and use of prenatal steroid hormone...
November 2017: Zhongguo Dang Dai Er Ke za Zhi, Chinese Journal of Contemporary Pediatrics
https://www.readbyqxmd.com/read/29129877/evaluation-of-the-chemiluminescent-enzyme-immunoassay-system-for-the-measurement-of-testosterone-in-the-serum-and-whole-blood-of-stallions
#6
Yuko Toishi, Nobuo Tsunoda, Shun-Ichi Nagata, Rikio Kirisawa, Kentaro Nagaoka, Gen Watanabe, Yojiro Yanagawa, Seiji Katagiri, Kazuyoshi Taya
Testosterone (T) concentration is a useful indicator of reproductive function in male animals. However, T concentration is not usually measured in veterinary clinics, partly due to the unavailability of reliable and rapid assays for animal samples. In this study, a rapid chemiluminescent enzyme immunoassay system (CLEIA system) that was developed for the measurement of T concentration in humans use was validated for stallion blood samples. First, serum T concentrations were measured using the CLEIA system and compared with those measured by a fluoroimmunoassay that has been validated for use in stallions...
November 11, 2017: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/29123427/effect-of-age-and-estrogen-on-biochemical-markers-of-bone-turnover-in-postmenopausal-women-a-population-based-study-from-nepal
#7
Bashu Dev Pardhe, Sabala Pathak, Anjeela Bhetwal, Sumitra Ghimire, Shreena Shakya, Puspa Raj Khanal, Sujan Babu Marahatta
Background: Osteoporosis, a common disease worldwide, is characterized by low bone mass and architectural deterioration of bone tissue, leading to enhanced bone fragility and increase in fracture risks. Both menopause and aging are related factors leading to greater risk of bone disease, particularly among postmenopausal women. Hence, the main purpose of this study was to investigate the differences in biochemical markers of bone turnover and to evaluate the association of hormones and age-related factors with biochemical markers between pre- and postmenopausal women...
2017: International Journal of Women's Health
https://www.readbyqxmd.com/read/29118518/insignificant-correlation-between-thyroid-hormone-and-antithyroid-peroxidase-antibodies-in-alopecia-areata-patients-in-northern-rajasthan
#8
Gagandeep Kaur, C M Kuldeep, Puneet Bhargava, Deepak Kumar Mathur, Sonam Sharda, Pulkit Chaturvedi
Background: Alopecia areata (AA) is a common, recurrent form of nonscarring alopecia which often presents as circumscribed patches of spontaneous hair loss. The global prevalence of this disease varies from 0.1% to 0.2% in general population and 7-30 cases per 1000 dermatological patients. The etiology of AA still remains uncertain; however, genetic or environmental factor and autoimmunity are claimed responsible for it. Various autoimmune diseases, such as Hashimoto's thyroiditis, diabetes mellitus, vitiligo, and lupus erythematosus, have been reported in association with AA...
October 2017: International Journal of Trichology
https://www.readbyqxmd.com/read/29115801/the-relationship-between-the-vitamin-serum-25-oh-d-and-the-b12-concentrations-in-obese-women
#9
Nikola Curic, Branislava Ilincic, Natasa Milic, Velibor Cabarkapa, Stanislava Nikolic, Milica Medic-Stojanoska, Rinaldo Pellicano, Ludovico Abenavoli
BACKGROUND: In obesity, low levels of vitamin D (VD) and vitamin B12 (VB12), may be the result of different pathophysiological mechanisms, but the possible association between them has not yet been defined. The aim of this cross-sectional analysis was to investigate the possible relationship between serum 25-hydroxyvitamin D (25(OH)D) and VB12 levels in middle aged women. METHODS: In 80 women, we indirectly evaluated body composition and body volumes [extracellular fluid volume (ECV) and total body water (TBW)] by anthropometric and bioelectrical impedance analysis...
November 7, 2017: Minerva Medica
https://www.readbyqxmd.com/read/29115296/fully-automated-chemiluminescence-vs-ria-aldosterone-assay-in-primary-aldosteronism-work-up
#10
F Pizzolo, G Salvagno, B Caruso, C Cocco, F Zorzi, C Zaltron, A Castagna, L Bertolone, F Morandini, G Lippi, O Olivieri
Aldosterone and renin measurement is a cornerstone for primary aldosteronism (PA) diagnosis, but different thresholds are used according to different assays. A fully automated chemiluminescence (CL) immunoassay for renin and aldosterone was recently proposed, showing good performance for PA screening by aldosterone to renin ratio (ARR). This study aimed to define the accuracy of this assay in the screening and in the most popular confirmatory test of autonomous aldosterone production, the intravenous saline loading test (ivSLT)...
December 2017: Journal of Human Hypertension
https://www.readbyqxmd.com/read/29109862/comparative-analysis-of-thyroid-function-parameters-in-pregnant-women
#11
Feng Ren, Huan Zhou, Min Chen, Xianqiu Xiao, Xiaoping Rui
The aim of the present study was to investigate the serum levels of free triiodothyronine (FT3), free thyroxine (FT4) and thyroid stimulating hormone (TSH) during pregnancy and comparative analysis of serum markers levels in non-pregnant women and pregnant women. Pregnant women were divided into four groups according to their gestational age: 8-14, 15-20, 21-36 and ≥37 weeks. Non-pregnant women were divided into three groups according to their age: 20-40, 41-55 and 56-85 years, and women of reproductive age (20-40 years) as control, which match their age and body mass index with pregnant women...
November 2017: Biomedical Reports
https://www.readbyqxmd.com/read/29108365/a-3-year-observation-of-testosterone-deficiency-in-chinese-patients-with-chronic-heart-failure
#12
Ying Han, Weiju Sun, Guizhi Sun, Xiaolu Hou, Zhaowei Gong, Jing Xu, Xiuping Bai, Lu Fu
Testosterone deficiency is present in a certain proportion men with chronic heart failure (CHF). Low testosterone levels in American and European patients with CHF lead to the high mortality and readmission rates. Interestingly, this relationship has not been studied in Chinese patients. To this end, 167 Chinese men with CHF underwent clinical and laboratory evaluations associated with determinations of testosterone levels. Total testosterone (TT) levels and sex hormone-binding globulin were measured by chemiluminescence or immunoassays assays and free testosterone (FT) levels were calculated, Based upon results from these assays, patients were divided into either a low testosterone (LT; n = 93) or normal testosterone (NT; n = 74) group...
October 3, 2017: Oncotarget
https://www.readbyqxmd.com/read/29099903/-repeatability-and-variability-of-total-t4-measurements-at-three-german-veterinary-laboratories
#13
Ralf Mueller, Teresa Böhm, Christoph Klinger, Janine Classen, Laura Udraite, Monika Linek, Ralf S Mueller
OBJECTIVE: To evaluate the reproducibility of serum testing for total thyroxine (T4) in three German laboratories. MATERIALS AND METHODS: Serum was taken from 53 dogs with suspected hypothyroidism and divided into five aliquots. Three aliquots of each sample were marked with different names and sent to one laboratory (two aliquots simultaneously and one aliquot 1-3 days later). The remaining aliquots were sent to two other laboratories. Laboratory 1 used an enzyme immunoassay for T4 measurements, whereas laboratories 2 and 3 used a chemiluminescence immunoassay...
November 3, 2017: Tierärztliche Praxis. Ausgabe K, Kleintiere/Heimtiere
https://www.readbyqxmd.com/read/29098964/misdiagnosis-of-graves-hyperthyroidism-due-to-therapeutic-biotin-intervention
#14
Yentl De Roeck, Eva Philipse, Theodorus B Twickler, Luc Van Gaal
BACKGROUND: Lately, high dose of biotin is often given orally to patients with a primary progressive multiple sclerosis (PPMS). However, the molecule biotin is also a principle compound in various analytic immunoassays. CLINICAL CASE: An asymptomatic 60-year-old woman with PPMS on high dose of biotin therapy (3 × 100 mg/d) displayed abnormal thyroid function tests (TSH 0.02 mU/l, fT4 > 103 pmol/l, and fT3 > 46 pmol/l). TSH was determined by a homogeneous sandwich chemiluminescent immunoassay and fT4 and fT3 were both determined by a homogeneous, sequential, chemiluminescent immunoassay...
November 3, 2017: Acta Clinica Belgica
https://www.readbyqxmd.com/read/29092879/eggs-early-in-complementary-feeding-increase-choline-pathway-biomarkers-and-dha-a-randomized-controlled-trial-in-ecuador
#15
Lora L Iannotti, Chessa K Lutter, William F Waters, Carlos Andres Gallegos Riofrío, Carla Malo, Gregory Reinhart, Ana Palacios, Celia Karp, Melissa Chapnick, Katherine Cox, Santiago Aguirre, Luis Narvaez, Fernando López, Rohini Sidhu, Pamela Kell, Xuntian Jiang, Hideji Fujiwara, Daniel S Ory, Rebecca Young, Christine P Stewart
Background: Choline status has been associated with stunting among young children. Findings from this study showed that an egg intervention improved linear growth by a length-for-age z score of 0.63.Objective: We aimed to test the efficacy of eggs introduced early in complementary feeding on plasma concentrations of biomarkers in choline pathways, vitamins B-12 and A, and essential fatty acids.Design: A randomized controlled trial, the Lulun ("egg" in Kichwa) Project, was conducted in a rural indigenous population of Ecuador...
November 1, 2017: American Journal of Clinical Nutrition
https://www.readbyqxmd.com/read/29091300/serologic-and-viral-genome-prevalence-of-hsv-ebv-and-hcmv-among-healthy-adults-in-wuhan-china
#16
Le Wen, Yong Qiu, Shuang Cheng, Xuan Jiang, Yan-Ping Ma, Wei Fang, Wei Wang, Jie Cui, Qiang Ruan, Fei Zhao, Fei Hu, Min-Hua Luo
The worldwide infection rate of herpesvirus is high, but the detailed prevalence in China, especially the central area, remains unclear. In the present study, the prevalence of Herpes simplex virus (HSV), Epstein-Barr virus (EBV), and human cytomegalovirus (HCMV) was investigated in 303 healthy adults in Wuhan, a representative city in Central China. Viral specific IgG and IgM titers were examined in the serum by chemiluminescent immunoassay, and the existence of viral genomic DNA in blood cells were determined by nested PCR...
November 1, 2017: Journal of Medical Virology
https://www.readbyqxmd.com/read/29081654/association-between-serum-%C3%AE-2-microglobulin-levels-and-frailty-in-an-elderly-chinese-population-results-from-rulas
#17
Zu-Yun Liu, Yan-Yu Shen, Li-Juan Ji, Xiao-Yan Jiang, Xiao-Feng Wang, Yan Shi
OBJECTIVE: To examine the association between serum β2-microglobulin (B2M) levels and frailty in an elderly Chinese population. DESIGN: A population-based cohort study. SETTING AND PARTICIPANTS: We used data on 1,663 elderly participants (aged 70-84 years) from the aging arm of the Rugao Longevity and Ageing study, a population-based observational two-arm cohort study conducted in Rugao, China. MEASUREMENTS: The serum B2M was measured with chemiluminescence immunoassay by a technician in the biochemistry laboratory of the Rugao People's Hospital...
2017: Clinical Interventions in Aging
https://www.readbyqxmd.com/read/29075534/nt-probnp-cardiometabolic-risk-factors-and-nutritional-status-in-hemodialysis-patients
#18
Jacques Ducros, Laurent Larifla, Henri Merault, Lydia Foucan
BACKGROUND: We aimed to evaluate the association between NT-proBNP and malnutrition in HD patients while taking into account the four established categories of parameters for diagnosis of protein energy wasting (PEW). METHODS: A cross-sectional study was performed in Afro-Caribbean dialysis patients. One component in each of the 4 categories for the wasting syndrome was retained: serum albumin ≤ 38 g/L, BMI ≤ 23 Kg/m(2), serum creatinine ≤ 818 µmol/L, and normalized protein catabolic rate (nPCR) ≤ 0...
2017: International Journal of Nephrology
https://www.readbyqxmd.com/read/29065856/factors-associated-to-serum-25-hydroxyvitamin-d-levels-among-older-adult-populations-in-urban-and-suburban-communities-in-shanghai-china
#19
Qun Cheng, Yanping Du, Wei Hong, Wenjing Tang, Huilin Li, Minmin Chen, Songbai Zheng
BACKGROUND: Vitamin D deficiency is widespread in China, particularly among older adults. Factors associated with suboptimal vitamin D levels are not well defined. The present study was a population-based study that included 10 urban and suburban communities in Shanghai, to evaluate vitamin D status and its correlates among older adults. METHOD: This cross-sectional study was based on study data for 3924 healthy men and women aged 65-95 years. Anthropometric and socioeconomic data were collected in June-July 2014...
October 24, 2017: BMC Geriatrics
https://www.readbyqxmd.com/read/29060990/-relationship-between-hbeag-from-hbsag-positive-mothers-and-regulatory-t-cells-in-neonates-and-its-influence-on-hbv-intrauterine-transmission
#20
H Y Hao, Z Q Yang, X X Xu, X F Wang, B Wang, X H Shi, Z D Fu, B Wang, S P Wang
Objective: To explore the relationship between HBeAg in HBsAg positive mothers and CD(4)(+)CD(25)(+)Foxp3(+)regulatory T cells (Treg) in newborns, as well as how they would influence the increasing risk on HBV intrauterine transmission. Methods: We collected information on general demographic characteristics and delivery on 270 HBsAg positive mothers and their newborns from the Third People's Hospital of Taiyuan. Fluorescence quantitative polymerase chain reaction (FQ-PCR) and chemiluminescence immunoassay (CLIA) were used to detect HBV DNA and HBV serological markers in peripheral blood from both mothers and neonates...
October 10, 2017: Zhonghua Liu Xing Bing Xue za Zhi, Zhonghua Liuxingbingxue Zazhi
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