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Cancer stemcell

Guangchuan Wang, Guang Yu, Dongmei Wang, Shengnan Guo, Fengping Shan
Natural killer (NK) cells are innate lymphocytes that aid in the protection of the host from infectious diseases and cancer. In vitro studies of NK cells have provided a foundation for developing clinical adoptive NK-cell transferred immunotherapy against human tumors. To elucidate the functions and mechanisms of NK cell populations, it is important to develop an optimal, highly reproducible and reliable isolation method. The present comparative study was performed with four different NK cell isolation kits of magnetic bead labeling made by Miltenyi and Stemcell companies, including positive selection kits [cluster of differentiation (CD)-49b, using the monoclonal antibody DX5) MicroBeads] and negative selection kits...
May 2017: Experimental and Therapeutic Medicine
Maren Bredemeier, Sabine Kasimir-Bauer, Hans-Christian Kolberg, Thomas Herold, Sarah Synoracki, Siegfried Hauch, Philippos Edimiris, Agnes Bankfalvi, Mitra Tewes, Rainer Kimmig, Bahriye Aktas
The aim of the present study was to compare the phosphatidylinositol 3-kinase (PI3KCA)-AKT serine/threonine kinase (AKT) pathway in circulating tumor cells (CTCs) and corresponding cancerous tissues. Stemness‑like circulating tumor cells (slCTCs) and CTCs in epithelial-mesenchymal transition (EMT) have been implicated as the active source of metastatic spread in breast cancer (BC). In this regard, the PI3KCA‑AKT signaling pathway was demonstrated to be implicated in and to be frequently mutated in BC. The present study compared this pathway in slCTCs/CTCs in EMT and the corresponding tumor tissues of 90 metastatic BC patients (pts)...
May 2017: Molecular Medicine Reports
Maren Bredemeier, Philippos Edimiris, Mitra Tewes, Pawel Mach, Bahriye Aktas, Doreen Schellbach, Jenny Wagner, Rainer Kimmig, Sabine Kasimir-Bauer
BACKGROUND: Circulating tumor cells (CTC) are discussed to be an ideal surrogate marker for individualized treatment in metastatic breast cancer (MBC) since metastatic tissue is often difficult to obtain for repeated analysis. We established a nine gene qPCR panel to characterize the heterogeneous CTC population in MBC patients including epithelial CTC, their receptors (EPCAM, ERBB2, ERBB3, EGFR) CTC in Epithelial-Mesenchymal-Transition [(EMT); PIK3CA, AKT2), stem cell-like CTC (ALDH1) as well as resistant CTC (ERCC1, AURKA] to identify individual therapeutic targets...
July 5, 2016: Oncotarget
Marie Klintman, Richard Buus, Maggie Chon U Cheang, Amna Sheri, Ian E Smith, Mitch Dowsett
PURPOSE: The primary aim was to derive evidence for or against the clinical importance of several biologic processes in patients treated with neoadjuvant chemotherapy (NAC) by assessing expression of selected genes with prior implications in prognosis or treatment resistance. The secondary aim was to determine the prognostic impact in residual disease of the genes' expression. EXPERIMENTAL DESIGN: Expression levels of 24 genes were quantified by NanoString nCounter on formalin-fixed paraffin-embedded residual tumors from 126 patients treated with NAC and 56 paired presurgical biopsies...
May 15, 2016: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
Pavlina Janovska, Lucie Poppova, Karla Plevova, Hana Plesingerova, Martin Behal, Marketa Kaucka, Petra Ovesna, Michaela Hlozkova, Marek Borsky, Olga Stehlikova, Yvona Brychtova, Michael Doubek, Michaela Machalova, Sivasubramanian Baskar, Alois Kozubik, Sarka Pospisilova, Sarka Pavlova, Vitezslav Bryja
PURPOSE: ROR1, a receptor in the noncanonical Wnt/planar cell polarity (PCP) pathway, is upregulated in malignant B cells of chronic lymphocytic leukemia (CLL) patients. It has been shown that the Wnt/PCP pathway drives pathogenesis of CLL, but which factors activate the ROR1 and PCP pathway in CLL cells remains unclear. EXPERIMENTAL DESIGN: B lymphocytes from the peripheral blood of CLL patients were negatively separated using RosetteSep (StemCell) and gradient density centrifugation...
January 15, 2016: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
The development of the discipline Obstetrics and Gynecology is reflected by the medical history at the University of Basel. At the very beginning of academic obstetrics, the cantonal government realised the need of a more practical teaching completing the tradition of theoretical lecturing (1865). Obstetrics was (and still is) a typical example for the turn from theoretical to hands-on-teaching in medicine. At the time, when the independent chair of obstetrics at the University of Basel was founded (1867, Bischoff [1841-1892]), the fight against puerperal fever was the most urgent aim...
December 1, 2002: Therapeutische Umschau. Revue Thérapeutique
Keiyo Takubo, Toshio Suda
Stem cells exhibit a number of characteristic features, including the capacity for self-renewal and differentiation into multiple cell types, stress resistance, and drug efflux activity. These specific biological characteristics are supported by signals from the surrounding niche and the stemcell-specific transcription factor set, including hypoxia and the machinery that senses low oxygen levels. These properties are essential for normal stem cells, and when defective may induce cellular senescence and tumorigenesis...
May 2012: International Journal of Hematology
Puja Gaur, Eric L Sceusi, Shaija Samuel, Ling Xia, Fan Fan, Yunfei Zhou, Jia Lu, Federico Tozzi, Gabriel Lopez-Berestein, Pablo Vivas-Mejia, Asif Rashid, Jason B Fleming, Eddie K Abdalla, Steven A Curley, Jean-Nicolas Vauthey, Anil K Sood, James C Yao, Lee M Ellis
BACKGROUND & AIMS: Metastatic gastrointestinal neuroendocrine tumors (NETs) frequently are refractory to chemotherapy. Chemoresistance in various malignancies has been attributed to cancer stem cells (CSCs). We sought to identify gastrointestinal neuroendocrine CSCs (N-CSCs) in surgical specimens and a NET cell line and to characterize novel N-CSC therapeutic targets. METHODS: Human gastrointestinal NETs were evaluated for CSCs using the Aldefluor (Stemcell Technologies, Vancouver, Canada) assay...
November 2011: Gastroenterology
Christine Beeton, K George Chandy
Natural killer (NK) cells are large granular cytotoxic lymphocytes that belong to the innate immune system and play major roles in fighting against cancer and infections, but are also implicated in the early stages of pregnancy and transplant rejection. These cells are present in peripheral blood, from which they can be isolated. Cells can be isolated using either positive or negative selection. For positive selection we use antibodies directed to a surface marker present only on the cells of interest whereas for negative selection we use cocktails of antibodies targeted to surface markers present on all cells but the cells of interest...
2007: Journal of Visualized Experiments: JoVE
B Griessmeier
The following paper is the manuscript of an oral presentation during the annual GPOH meeting on May 22nd, 2000 in Berlin. Following her experiences as a member of the psychosocial care team of a children's cancer unit the author discusses the emotional impact of stemcell or bone marrow transplantation in children. Psychosocial care is identified as a way to support children emotionally during a situation of existential crisis, and to help them share their inner feelings and experiences.
July 2001: Klinische Pädiatrie
A M Westermann, M M Holtkamp, G A Linthorst, L van Leeuwen, E J Willemse, W C van Dijk, W J Nooijen, J W Baars, J H Schornagel, S Rodenhuis
BACKGROUND: After high-dose chemotherapy with autologous stem-cell support long hospital stays in the aplastic phase are expensive, lead to increased risk of hospital infections and to increasing pressure on available hospital beds. We developed a home care regimen that allows patients to be at home for most of the aplastic period, without daily hospital visits. PATIENTS AND METHODS: Between October 1995 and December 1997, transfer of supportive care to the home setting took place in three phases for patients undergoing high-dose chemotherapy with stem-cell transplant for malignant lymphoma (one course of BEAM), breast cancer or germ-cell cancer (three courses of tCTC)...
May 1999: Annals of Oncology: Official Journal of the European Society for Medical Oncology
R D Blumenthal, R Alisauskas, M Juweid, R M Sharkey, D M Goldenberg
BACKGROUND: Single doses of radioantibody are effective at treating single cells or small clusters of cancer cells. However, large tumor masses require either multiple doses of radioantibody or a multimodal approach to therapy using two or more therapeutic agents. Timing of the second dose in a multiple cycle scheme or the second treatment in a multimodal protocol will depend on recovery from toxicity associated with the first treatment. METHODS: BALB/c mice were dosed with a maximal tolerated dose (MTD) of I-131-MN-14 anti-carcinoembryonic antigen immunoglobulin G (IgG) (250 microCi) or F(ab')2 (1...
December 15, 1997: Cancer
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