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dbs viral load

Fabien Taieb, Tran Hong Tram, Hien Thi Ho, Van Anh Pham, Lan Nguyen, Ban Hien Pham, Linh An Tong, Edouard Tuaillon, Eric Delaporte, Anh Tuan Nguyen, Duc Duong Bui, NhanThi Do, Yoann Madec
Background.  Although it is the best method to detect early therapeutic failure, viral load (VL) monitoring is still not widely available in many resource-limited settings because of difficulties in specimen transfer, personnel shortage, and insufficient laboratory infrastructures. Dried blood spot (DBS) use, which was introduced in the latest World Health Organization recommendations, can overcome these difficulties. This evaluation aimed at validating VL measurement in DBS, in a laboratory without previous DBS experience and in routine testing conditions...
September 2016: Open Forum Infectious Diseases
Brunna Lemos Crespo Marques, Márcia Paschoal do Espírito-Santo, Vanessa Alves Marques, Juliana Custódio Miguel, Elisangela Ferreira da Silva, Cristiane Alves Villela-Nogueira, Lia Laura Lewis-Ximenez, Elisabeth Lampe, Livia Melo Villar
BACKGROUND: Dried blood spots (DBS) could be an excellent alternative for HCV diagnosis, since it is less invasive and can be stored and transported without refrigeration. OBJECTIVES: The aim of this study was to optimize quantitative and qualitative methods for HCV detection in DBS. STUDY DESIGN: DBS and serum samples were collected from 99 subjects (59 anti-HCV/HCV RNA positive and 40 seronegative samples). Seven extraction methods and different PCR parameters were evaluated in DBS samples in the quantitative RT-PCR (qRT-PCR) developed to amplify the 5' noncoding region of HCV...
September 2016: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
Pierre de Truchis, Minh Patrick Lê, Mamane Daou, Boubacar Madougou, Yacouba Nouhou, Sahada Moussa Saley, Achirou Sani, Eric Adehossi, Elisabeth Rouveix, Mamadou Saidou, Gilles Peytavin, Constance Delaugerre
OBJECTIVES: The objectives of this study were to determine the rate of viral success in HIV-infected patients on first-line ART by the assessment of dried blood spot (DBS) viral load (VL) and to assess the performance of DBS sampling for VL measurement, genotypic resistance and antiretroviral concentration determinations. METHODS: HIV-infected patients treated for >1 year with first-line ART in Niamey, Niger were included. VL based on nucleic acid sequence-based amplification (NASBA) assay (limit of quantification <800 copies/mL) was measured on DBS capillary samples...
July 20, 2016: Journal of Antimicrobial Chemotherapy
Sheri A Lippman, Starley B Shade, Alison M El Ayadi, Jennifer M Gilvydis, Jessica S Grignon, Teri Liegler, Jessica Morris, Evasen Naidoo, Lisa M Prach, Adrian Puren, Scott Barnhart
BACKGROUND: Attrition along the HIV care continuum slows gains in mitigating the South African HIV epidemic. Understanding population-level gaps in HIV identification, linkage, retention in care, and viral suppression is critical to target programming. METHODS: We conducted a population-based household survey, HIV rapid testing, point-of-care CD4 testing, and viral load measurement from dried blood spots using multistage cluster sampling in 2 subdistricts of North West Province from January to March, 2014...
September 1, 2016: Journal of Acquired Immune Deficiency Syndromes: JAIDS
Sheila N Balinda, Pascale Ondoa, Ekwaro A Obuku, Aletta Kliphuis, Isaac Egau, Michelle Bronze, Lordwin Kasambula, Rob Schuurman, Nicole Spieker, Tobias F Rinke de Wit, Cissy Kityo
BACKGROUND: WHO recommends regular viral load (VL) monitoring of patients on antiretroviral therapy (ART) for timely detection of virological failure, prevention of acquired HIV drug resistance (HIVDR) and avoiding unnecessary switching to second-line ART. However, the cost and complexity of routine VL testing remains prohibitive in most resource limited settings (RLS). We evaluated a simple, low-cost, qualitative viral-failure assay (VFA) on dried blood spots (DBS) in three clinical settings in Uganda...
2016: PloS One
Fulvio Erba, Davide Brambilla, Susanna Ceffa, Fausto Ciccacci, Richard Luhanga, Zita Sidumo, Leonardo Palombi, Sandro Mancinelli, Maria Cristina Marazzi, Mauro Andreotti, Marina Giuliano
BACKGROUND: The use of dried blood spots (DBS) for HIV-1 viral load quantification can greatly improve access to viral monitoring for HIV-infected patients receiving treatment in resource-limited settings. OBJECTIVES: To evaluate and validate HIV viral load measurement from DBS in sub-Saharan Africa, with a reliable, all-automated, standard commercial assay such as the Abbott m2000. METHODS: A total of 277 DBS were collected in different health centres in Malawi and Mozambique and analysed for viral load determination using the Abbott m2000 assay with the corresponding plasma samples as gold standard...
December 2015: South African Medical Journal, Suid-Afrikaanse Tydskrif Vir Geneeskunde
O Ndiaye, J Gozlan, H Diop-Ndiaye, A S Sall, S Chapelain, A Leprêtre, M Maynart, M Gueye, G Lo, M Thiam, I Ba, K Lacombe, P M Girard, S Mboup, C T Kane
The aim of this pilot study was to analyze the Hepatitis C Virus (HCV) genotypes circulating in Senegal among Drug User (DUs), using Dried Blood Spots (DBS) as RNA source for molecular assays. Heroin and/or cocaine users (n = 506) were recruited in Dakar from April to July 2011, using a Respondent Driven Sampling (RDS) method. DBS preparation consisted of five drops of whole blood from finger applied to a Whatman paper card. HCV infection was screened by the detection of anti-HCV antibodies, using a rapid immune-chromatographic test...
December 24, 2015: Journal of Medical Virology
François Boillot, Laetitia Serrano, Jeremie Muwonga, Jean Pierre Kabuayi, Alain Kambale, Fidèle Mutaka, Paula I Fujiwara, Josef Decosas, Martine Peeters, Eric Delaporte
BACKGROUND: As part of its policy to shift monitoring of antiretroviral therapy (ART) to primary health care (PHC) workers, the Ministry of Health of the Democratic Republic of Congo (DRC) tested the feasibility of using dried blood spots (DBS) for viral load (VL) quantification and genotypic drug resistance testing in off-site high-throughput laboratories. METHODS: DBS samples from adults on ART were collected in 13 decentralized PHC facilities in the Nord-Kivu province and shipped during program quarterly supervision to a reference laboratory 2000 km away, where VL was quantified with a commercial assay (m2000rt, Abbott)...
January 1, 2016: Journal of Acquired Immune Deficiency Syndromes: JAIDS
Michael J Vinikoor, Samuel Zürcher, Kalo Musukuma, Obert Kachuwaire, Andri Rauch, Benjamin H Chi, Meri Gorgievski, Marcel Zwahlen, Gilles Wandeler
BACKGROUND: Access to hepatitis B viral load (VL) testing is poor in sub-Saharan Africa (SSA) due to economic and logistical reasons. OBJECTIVES: To demonstrate the feasibility of testing dried blood spots (DBS) for hepatitis B virus (HBV) VL in a laboratory in Lusaka, Zambia, and to compare HBV VLs between DBS and plasma samples. STUDY DESIGN: Paired plasma and DBS samples from HIV-HBV co-infected Zambian adults were analyzed for HBV VL using the COBAS AmpliPrep/COBAS TaqMan HBV test (Version 2...
November 2015: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
Patricia Alvarez, Leticia Martín, Luis Prieto, Jacinta Obiang, Antonio Vargas, Pedro Avedillo, Pablo Rojo, Carolina Fernández McPhee, Agustín Benito, José Tomás Ramos, África Holguín
OBJECTIVES: Viral load (VL) testing is used for early HIV diagnosis in infants (EID) and for detecting early therapeutic failure events, but can be affected by HIV genetic variability. Dried blood samples (DBS) increase VL access and EID in remote settings and when low blood volume is available. METHODS: This study compares VL values using Siemens VERSANT HIV-1 RNA 1.0 kPCR assay (kPCR) and Roche CAP/CTM Quantitative test v2.0 (CAP/CTM v2.0) in 176 DBS carrying different HIV-1 variants collected from 69 Equatoguinean mothers and their infants with known HIV-1 status (71 infected, 105 uninfected)...
September 2015: Journal of Infection
Sue Napierala Mavedzenge, Calum Davey, Tarisai Chirenje, Phyllis Mushati, Sibongile Mtetwa, Jeffrey Dirawo, Boniface Mudenge, Andrew Phillips, Frances M Cowan
BACKGROUND: In the context of a community-randomized trial of antiretrovirals for HIV prevention and treatment among sex workers in Zimbabwe (the SAPPH-IRe trial), we will measure the proportion of women with HIV viral load (VL) above 1000 copies/mL ("VL>1000") as our primary endpoint. We sought to characterize VL assay performance by comparing results from finger prick dried blood spots (DBS) collected in the field with plasma samples, to determine whether finger prick DBS is an acceptable sample for VL quantification in the setting...
2015: PloS One
Sarah E Rutstein, Mina C Hosseinipour, Deborah Kamwendo, Alice Soko, Memory Mkandawire, Andrea K Biddle, William C Miller, Morris Weinberger, Stephanie B Wheeler, Abdoulaye Sarr, Sundeep Gupta, Frank Chimbwandira, Reuben Mwenda, Steve Kamiza, Irving Hoffman, Ronald Mataya
OBJECTIVES: To evaluate the feasibility and effectiveness of dried blood spots (DBS) use for viral load (VL) monitoring, describing patient outcomes and programmatic challenges that are relevant for DBS implementation in sub-Saharan Africa. METHODS: We recruited adult antiretroviral therapy (ART) patients from five district hospitals in Malawi. Eligibility reflected anticipated Ministry of Health VL monitoring criteria. Testing was conducted at a central laboratory...
2015: PloS One
Sivapragashini Danaviah, Tulio de Oliveira, Ruth Bland, Johannes Viljoen, Sureshnee Pillay, Edouard Tuaillon, Philippe Van de Perre, Marie-Louise Newell
Exposure of the infant's gut to cell-associated and cell-free HIV-1 trafficking in breast milk (BM) remains a primary cause of mother-to-child transmission (MTCT). The mammary gland represents a unique environment for HIV-1 replication and host-virus interplay. We aimed to explore the origin of the virus transmitted during breastfeeding, and the link with quasi-species found in acellular and cellular fractions of breast-milk (BM) and in maternal plasma. The C2-V5 region of the env gene was amplified, cloned and sequenced from the RNA and DNA of BM, the RNA from the mother's plasma (PLA) and the DNA from infant's dried blood spot (DBS) in 11 post-natal mother-infant pairs...
2015: PloS One
Xiaoning Wu, Megan Crask, Hector Ramirez, Trevor Landas, Tri D Do, Christiane Honisch, Stephen Will, Paul D Baum
BACKGROUND: Dried blood spots (DBS) improve access to HIV viral load (VL) testing, but yield increased VL measurements compared to the plasma reference method because of cell-associated viral nucleic acid. In clinical settings, DBS methods may falsely categorize many patients as failing therapy. OBJECTIVES: Description of a simple method, free virus elution (FVE), to preferentially elute plasma-associated virus from DBS samples with phosphate-buffered saline, and an initial HIV VL performance comparison with standard DBS elution methods...
April 2015: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
Abou Abdallah Malick Diouara, Halimatou Diop Ndiaye, Ibrehima Guindo, Nestor Bangoura, Mohamed Cissé, Tchiakpe Edmond, Flabou Bougoudogo, Souleymame Mboup, Martine Peeters, Ahidjo Ayouba, Ndèye Coumba Touré Kane
INTRODUCTION: Access to antiretroviral treatment (ART) becomes more and more effective in resource-limited settings (RLS). However, this global effort would be even more profitable if the access to laboratory services especially in decentralized settings was strengthened. We report the virological outcome and HIV-1 drug resistance in three West African countries using dried blood spots (DBS) samples. METHODS: We included HIV-1-infected adults on ART ≥6 months and followed up in capital cities and decentralized sites in Senegal, Mali and Guinea-Conakry...
2014: Journal of the International AIDS Society
Hai Zhao, Lingjie Liao, Hui Xing, Zhe Wang, Bin Su, Jia Liu, JianJun Wu, Yi Feng, Cui He, Yuhua Ruan, Yiming Shao
OBJECTIVES: We evaluated an In-house assay for HIV-1 drug resistance genotyping by using DBS samples in China. METHODS: The amplification sensitivity was assessed using 79 DBS specimens with plasma viral load ranging from 1,000 to 6,000 copies/ml. Precision was assessed using 5 DBS specimens with 5 replicates tested in one test run. Reproducibility was evaluated using other 5 DBS specimens with 5 replicates genotyped in 5 test runs. Nucleotide sequence identity and the degree of concordance in detecting drug resistance mutations were assessed within and between test runs...
2015: Current HIV Research
Rami Kantor, Allison DeLong, Maya Balamane, Leeann Schreier, Robert M Lloyd, Wilfred Injera, Lydia Kamle, Fidelis Mambo, Sarah Muyonga, David Katzenstein, Joseph Hogan, Nathan Buziba, Lameck Diero
INTRODUCTION: Antiretroviral resistance leads to treatment failure and resistance transmission. Resistance data in western Kenya are limited. Collection of non-plasma analytes may provide additional resistance information. METHODS: We assessed HIV diversity using the REGA tool, transmitted resistance by the WHO mutation list and acquired resistance upon first-line failure by the IAS-USA mutation list, at the Academic Model Providing Access to Healthcare (AMPATH), a major treatment programme in western Kenya...
2014: Journal of the International AIDS Society
James Kimaro, Elichilia Shao, Balthazar Nyombi, Emanuel Kifaro, Dorcas Maruapula, Simani Gaseitsiwe, Rosemary Musonda
INTRODUCTION: A dried blood spot (DBS) on filter paper has been used for different tests globally and has gained popularities in resource limited settings especially during HIV/AIDS epidemic. We assessed the efficiency of molecular characterization of HIV-1 subtypes using DBS collected under field conditions in northern Tanzania. MATERIALS AND METHODS: In 2011 and 2012, 60 DBS samples were collected under field conditions from exposed and newly diagnosed HIV-1 infected children from Kilimanjaro (n=20), Arusha (n=20), Tanga (n=10) and Manyara (n=10)...
2014: Journal of the International AIDS Society
Andrea Calcagno, Ilaria Motta, Maria Grazia Milia, Roberto Rostagno, Marco Simiele, Valentina Libanore, Silvia Fontana, Antonio D'Avolio, Valeria Ghisetti, Giovanni Di Perri, Stefano Bonora
AIMS: In limited resource settings monitoring antiretroviral (ARV) treatment efficacy is restrained by the lack of access to technological equipment. The aim of the study was to assess the use of dried plasma (DPS) and blood spots (DBS) to facilitate ARV monitoring in remote settings where clinical monitoring is the primary strategy. METHODS: A cross-sectional study in HIV-positive ARV-treated patients in Kiremba, Burundi was performed. DBS were used for HIV-1 viral load (limit of the assay 250 copies ml(-1)) and genotypic drug resistance tests and dried plasma spots were used for concentration measurements...
May 2015: British Journal of Clinical Pharmacology
Jamie Greenman, Teri Roberts, Jennifer Cohn, Luke Messac
The entry of new all-oral direct acting antiviral therapy for hepatitis C provides an opportunity to scale up HCV care in low- and middle-income countries. In HIV, use of dried blood spots (DBS) has facilitated the diagnosis and management of HIV in resource-poor settings. DBS may be used in a similar way to facilitate diagnosis and management of HCV. Here, we present a systematic review of the literature of DBS for HCV RNA detection and genotyping. Using an a priori review protocol, three databases were searched for studies published up to August 2013 that reported the use of dried blood and serum spots in genotyping, detection and measurement of HCV RNA, as well as the rate of degradation of HCV RNA when stored in DBS at room temperature...
April 2015: Journal of Viral Hepatitis
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