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https://www.readbyqxmd.com/read/27911694/seroprevalence-of-babesia-microti-in-individuals-with-lyme-disease
#1
Sabino R Curcio, Laurel P Tria, Azad L Gucwa
INTRODUCTION: Babesiosis is an emerging tick-borne disease (TBD) caused by Babesia microti, an intracellular parasite of red blood cells. Currently, it is the highest ranked pathogen transmitted by blood transfusion. Most healthy individuals infected with B. microti are asymptomatic, but may be at risk for chronic infection. Similar to Lyme disease transmitted by Borrelia burgdorferi, B. microti is spread by Ixodes scapularis ticks. The rate of coinfection with these TBDs in humans is unclear as most studies have focused their prevalence in ticks or rodent reservoirs...
December 2016: Vector Borne and Zoonotic Diseases
https://www.readbyqxmd.com/read/27907198/a-molecular-approach-applied-to-enteroviruses-surveillance-in-northern-taiwan-2008-2012
#2
Wan-Yu Chung, Pai-Shan Chiang, Shu-Ting Luo, Tzou-Yien Lin, Kuo-Chien Tsao, Min-Shi Lee
Traditional methods for detection and serotyping of enterovirus infections are virus isolation and immunofluorescence assay (VI-IFA), which are labor-intensive and time-consuming. Recently, VP1 gene has been targeted to develop a CODEHOP-based RT-PCR (VP1-CODEHOP) for the same purpose. In this study, we conducted a 5-year enterovirus surveillance comparing the VI-IFA and VP1-CODEHOP tests. Throat swabs were collected from 431 pediatric patients and 208(48%) and 250(58%) were tested positive by the VI-IFA and VP1-CODEHOP tests, respectively...
2016: PloS One
https://www.readbyqxmd.com/read/27905278/seroprevalence-of-canine-leishmaniasis-in-northern-cyprus
#3
Tayfun Çanakçı, Arif Kurtdede, Serdar Paşa, Seray Töz Özensoy, Yusuf Özbel
OBJECTIVE: Canine leishmaniasis (CanL) is an important public and veterinary health problem in Mediterranean Basin countries. In this study, we aimed to determine the seroprevalence of CanL in several provinces of Northern Cyprus. METHODS: The seroprevalence of CanL was determined by the indirect fluorescent antibody (IFA) test in dog sera. In total, 281 dogs were randomly selected from Nicosia (n=80), Trikomo (n=58), Famagusta (n=60), Morphou (n=30), and Kyrenia (n=53), consistent with a statistically representative number of the regional dog population...
September 2016: Türkiye Parazitolojii Dergisi
https://www.readbyqxmd.com/read/27893414/recombinant-haemonchus-contortus-24-kda-excretory-secretory-protein-rhces-24-modulate-the-immune-functions-of-goat-pbmcs-in-vitro
#4
Javaid Ali Gadahi, Baojie Li, Muhammad Ehsan, Shuai Wang, Zhenchao Zhang, Yujian Wang, Muhammad Waqqas Hasan, Ruofeng Yan, Xiaokai Song, Lixin Xu, Xiangrui Li
A 24 kDa protein is one of the important components in Haemonchus contortus (barber pole worm) excretory/secretory products (HcESPs), which was shown to have important antigenic function. However, little is known about the immunomodulatory effects of this proteinon host cell. In the present study gene encoding 24kDa excretory/secretory protein (HcES-24) was cloned. The recombinant protein of HcES-24 (rHcES-24) was expressed in a histidine-tagged fusion protein soluble form in Escherichia coli. Binding activity of rHcES-24 to goat PBMCs was confirmed by immunofluorescence assay (IFA) and its immunomudulatory effect on cytokine secretion, cell proliferation, cell migration and nitric oxide production were observed by co-incubation of rHcES-24...
November 21, 2016: Oncotarget
https://www.readbyqxmd.com/read/27889321/rapid-antigen-detection-test-for-respiratory-syncytial-virus-diagnosis-as-a-diagnostic-tool
#5
Flávio da Silva Mesquita, Danielle Bruna Leal de Oliveira, Daniela Crema, Célia Miranda Nunes Pinez, Thaís Cristina Colmanetti, Luciano Matsumia Thomazelli, Alfredo Elias Gilio, Sandra Elisabeth Vieira, Marina Baquião Martinez, Viviane Fongaro Botosso, Edison Luiz Durigon
OBJECTIVE: The aim of this study was to evaluate the QuickVue(®) RSV Test Kit (QUIDEL Corp, CA, USA) as a screening tool for respiratory syncytial virus in children with acute respiratory disease in comparison with the indirect immunofluorescence assay as gold standard. In Brazil, rapid antigen detection tests for respiratory syncytial virus are not routinely utilized as a diagnostic tool, except for the diagnosis of dengue and influenza. METHODS: The authors retrospectively analyzed 486 nasopharyngeal aspirate samples from children under age 5 with acute respiratory infection, between December 2013 and August 2014, the samples were analyzed by IFI and QuickVue(®) RSV Test kit...
November 24, 2016: Jornal de Pediatria
https://www.readbyqxmd.com/read/27886586/nitrous-oxide-from-moving-bed-based-integrated-fixed-film-activated-sludge-membrane-bioreactors
#6
Giorgio Mannina, Marco Capodici, Alida Cosenza, Daniele Di Trapani, Vito Armando Laudicina, Hallvard Ødegaard
The present paper reports the results of a nitrous oxide (N2O) production investigation in a moving bed based integrated fixed film activated sludge (IFAS) membrane bioreactor (MBR) pilot plant designed in accordance with the University of Cape Town layout for biological phosphorous removal. Gaseous and liquid samples were collected in order to measure the gaseous as well as the dissolved concentration of N2O. Furthermore, the gas flow rate from each reactor was measured and the gas flux was estimated. The results confirmed that the anoxic reactor represents the main source of nitrous oxide production...
November 22, 2016: Journal of Environmental Management
https://www.readbyqxmd.com/read/27885561/outbreak-of-severe-pseudorabies-virus-infection-in-pig-offal-fed-farmed-mink-in-liaoning-province-china
#7
Hao Liu, Xin-Tong Li, Bo Hu, Xiao-Yu Deng, Lei Zhang, Shi-Zhen Lian, Hai-Ling Zhang, Shuang Lv, Xiang-Hong Xue, Rong-Guang Lu, Ning Shi, Ming-Hao Yan, Peng-Peng Xiao, Xi-Jun Yan
An outbreak of severe pseudorabies virus (PRV) infection in farmed mink occurred in northern China in late 2014, causing significant economic losses in the local fur industry. Here, we report the first case of a PRV outbreak in mink in northeastern China, caused by feeding farmed mink with raw pork or organs contaminated by PRV. Mink infected with virulent PRV exhibited diarrhea, neurologic signs, and higher mortality, which can be misdiagnosed as highly pathogenic mink enteritis virus (MEV), canine distemper virus (CDV), and food poisoning...
November 24, 2016: Archives of Virology
https://www.readbyqxmd.com/read/27884438/toxoplasma-gondii-in-stranded-marine-mammals-from-the-north-sea-and-eastern-atlantic-ocean-findings-and-diagnostic-difficulties
#8
Norbert van de Velde, Brecht Devleesschauwer, Mardik Leopold, Lineke Begeman, Lonneke IJsseldijk, Sjoukje Hiemstra, Jooske IJzer, Andrew Brownlow, Nicholas Davison, Jan Haelters, Thierry Jauniaux, Ursula Siebert, Pierre Dorny, Stéphane De Craeye
The occurrence of the zoonotic protozoan parasite Toxoplasma gondii in marine mammals remains a poorly understood phenomenon. In this study, samples from 589 marine mammal species and 34 European otters (Lutra lutra), stranded on the coasts of Scotland, Belgium, France, The Netherlands and Germany, were tested for the presence of T. gondii. Brain samples were analysed by polymerase chain reaction (PCR) for detection of parasite DNA. Blood and muscle fluid samples were tested for specific antibodies using a modified agglutination test (MAT), a commercial multi-species enzyme-linked immunosorbent assay (ELISA) and an immunofluorescence assay (IFA)...
October 30, 2016: Veterinary Parasitology
https://www.readbyqxmd.com/read/27884171/molecular-characterization-and-protective-efficacy-of-silent-information-regulator-2a-from-eimeria-tenella
#9
Hui Dong, Sihan Yang, Qiping Zhao, Hongyu Han, Shunhai Zhu, Xuelong Zhu, Cong Li, Ziwen Wang, Weili Xia, Qifei Men, Liangyu Yang, Bing Huang
BACKGROUND: Silent information regulator 2 (SIR2) proteins are a family of NAD + -dependent protein deacetylases that are considered potential targets for anti-parasitic agents. In this study, we cloned and characterized SIR2A of the protozoan parasite Eimeria tenella (EtSIR2A) and investigated its protective efficacy as a DNA vaccine. METHODS: The EtSIR2A gene encoding 33.37 kDa protein from E. tenella second-generation merozoites was cloned, and recombinant EtSIR2A protein (rEtSIR2A) was produced in an Escherichia coli expression system...
November 25, 2016: Parasites & Vectors
https://www.readbyqxmd.com/read/27876989/seroprevalence-of-toxoplasmosis-in-diabetic-pregnant-women-in-southwestern-of-iran
#10
Jasem Saki, Shahla Shafieenia, Masoud Foroutan-Rad
To investigate anti-Toxoplasma gondii IgG and IgM antibodies in diabetic pregnant women in Ahvaz, southwest of Iran this experiment was performed. In current study the sera of 110 diabetic pregnant women as well as 110 non diabetic pregnant women referred to the hospitals affiliated with the Ahvaz Jundishapur University of Medical Sciences were assessed for anti-T. gondii IgG and IgM antibodies by ELISA and IFA methods. The ELISA assessments showed that 47 (42.7 %) and 3 (2.7 %) of diabetic women were positive for IgG and IgM antibodies, respectively...
December 2016: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/27876003/pla2r-antibodies-and-pla2r-glomerular-deposits-in-psoriasis-patients-with-membranous-nephropathy
#11
Yong-Chun Ge, Bo Jin, Cai-Hong Zeng, Ming-Chao Zhang, Da-Cheng Chen, Ru Yin, Wei-Bo Le
BACKGROUND: The association between psoriasis and membranous nephropathy (MN) remains largely unclear. We examined the prevalence of serum PLA2R antibody and characterized the expression of PLA2R and THSD7A in glomeruli in patients with MN and psoriasis. METHODS: A total of 24 patients with MN without evidence of a secondary cause except psoriasis were enrolled. The clinical and pathological features were retrospectively analyzed. Serum anti-PLA2R antibody was measured using IFA Mosaic...
November 22, 2016: BMC Nephrology
https://www.readbyqxmd.com/read/27872568/weekly-iron-folic-acid-supplementation-plays-differential-role-in-maintaining-iron-markers-level-in-non-anaemic-and-anaemic-primigravida-a-randomized-controlled-study
#12
Hari Shankar, Neeta Kumar, Rajat Sandhir, Suneeta Mittal, Santosh Kurra, Lakhbir Dhaliwal, Gurjit Kaur, Nomita Chandhiok, B S Dhillon, D N Rao
: Anaemia during pregnancy is most commonly observed and highly prevalent in South-East Asia. Various effective programmes have been laid down for its management, mainly daily supplementation of iron folic acid (IFA) tablets. Following the same, standard obstetrical practice has included the IFA supplementation without requiring the determination of iron deficiency. In this study, a total of 120 primigravida (N = 60; non-anaemic (Hb > 11 g/dl) and N = 60 anaemic (Hb = 8-11 g/dl)) were selected among those attending the Antenatal Clinic in Department of Obstetrics and Gynaecology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India...
November 2016: Saudi Journal of Biological Sciences
https://www.readbyqxmd.com/read/27872103/rapid-and-continued-t-cell-differentiation-into-long-term-effector-and-memory-stem-cells-in-vaccinated-melanoma-patients
#13
Philippe Gannon, Petra Baumgaertner, Alexandre Huber, Emanuela M Iancu, Laurene Cagnon, Samia Abed-Maillard, Helene Maby-El Hajjami, Daniel E Speiser, Nathalie Rufer
PURPOSE: Cancer patients benefit increasingly from T cell-based therapies, such as adoptive T cell transfer, checkpoint blockade or vaccination. We have previously shown that serial vaccinations with Melan-AMART-126-35 peptide, CpG-B and IFA generated robust tumor-specific CD8 T cell responses in melanoma patients. Here, we describe the detailed kinetics of early- and long-term establishment of T cell frequency, differentiation (into memory and effector cells), poly-functionality and clonotype repertoire induced by vaccination...
November 21, 2016: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/27828620/prevalence-of-american-trypanosomiasis-and-leishmaniases-in-domestic-dogs-in-a-rural-area-of-the-municipality-of-s%C3%A3-o-jo%C3%A3-o-do-piau%C3%A3-piau%C3%A3-state-brazil
#14
Taliha Dias Perez, Fabiano Borges Figueiredo, Artur Augusto Mendes Velho Junior, Valmir Laurentino Silva, Maria de Fátima Madeira, Reginaldo Peçanha Brazil, José Rodrigues Coura
Chagas disease and the leishmaniases are endemic zoonoses of great importance to public health in the state of Piauí, Brazil. The domestic dog (Canis familiaris) is a major reservoir, host of Trypanosoma cruzi and Leishmania spp. in both urban and rural areas, playing an important role in the transmission of these parasites. The present study evaluated the prevalence of both infectious diseases in dogs of a rural area in the municipality of São João do Piauí, Piauí State. One hundred twenty-nine blood samples were collected for serological assessment: for the leishmaniases, 49 (38%) animals tested positive by the Dual-Path Platform technology (DPP), nine (6%) by the Enzyme-linked Immunosorbent Assay (ELISA), and 19 (14...
November 3, 2016: Revista do Instituto de Medicina Tropical de São Paulo
https://www.readbyqxmd.com/read/27812650/antibodies-for-rickettsia-spp-in-patients-with-negative-serology-for-dengue-virus-leptospirosis-and-meningococcal-disease-in-municipalities-of-s%C3%A3-o-paulo-state-brazil
#15
Juliana Anacleto Cabral Prata, Celso Eduardo de Souza, Rodrigo Nogueira Angerami, Taíse Marongio Cotrim de Moraes Barbosa, Fabiana Cristina Pereira Dos Santos, Silvia Colombo, Vânia Martins Fontes Del Guercio, Maria Rita Donalísio
INTRODUCTION: Brazilian spotted fever is an infectious disease with a high mortality rate if not treated early. Differential diagnosis is difficult, as the first clinical signs are non-specific and can be confused with other diseases. The aim of the study was to investigate evidence of infection with Rickettsia rickettsii and Rickettsia parkeri in negative sera samples, collected in 2014, from patients with suspected leptospirosis, dengue fever, and meningococcal disease in Atibaia and Bragança Paulista municipalities of the State of São Paulo...
September 2016: Revista da Sociedade Brasileira de Medicina Tropical
https://www.readbyqxmd.com/read/27798160/water-in-oil-only-adjuvants-selectively-promote-t-follicular-helper-cell-polarization-through-a-type-i-ifn-and-il-6-dependent-pathway
#16
Nicolas Riteau, Andrea J Radtke, Kevin Shenderov, Lara Mittereder, Sandra D Oland, Sara Hieny, Dragana Jankovic, Alan Sher
T follicular helper (Tfh) cells are a subset of CD4(+) T lymphocytes that promote the development of humoral immunity. Although the triggers required for the differentiation of the other major Th subsets are well defined, those responsible for Tfh cell responses are still poorly understood. We determined that mice immunized with peptide or protein Ags emulsified in IFA or related water-in-oil adjuvants develop a highly polarized response in which the majority of the Ag-specific CD4(+) T cells are germinal center-homing CXCR5(+)Bcl6(+) Tfh cells...
November 15, 2016: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/27790466/maternal-health-situation-in-bihar-and-madhya-pradesh-a-comparative-analysis-of-state-fact-sheets-of-national-family-health-survey-nfhs-3-and-4
#17
REVIEW
Ranjit Kumar Dehury, Janmejaya Samal
INTRODUCTION: Maternal health constitutes the health of women during pregnancy, childbirth and the postpartum period. Bihar and Madhya Pradesh (MP) constitute the Empowered Action Group (EAG) states under National Rural Health Mission (NRHM) and are consistently having poor maternal health indicators. AIM: The main objective of this study was to assess the maternal health situation of Bihar and MP based on National Family Health Survey (NFHS-3) and 4 fact sheets...
September 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/27790134/optical-coherence-tomography-angiography-of-iris-nevus-a-case-report
#18
Davide Allegrini, Giovanni Montesano, Alfredo Pece
Iris nevus is common: 6% of patients with suspected iris melanoma have lesions other than melanoma, and 36% of them are nevi. Iris nevus turns into melanoma in approximately 8% of cases at a mean of 15 years. This case report provides the first description of an iris tumor examined with iris optical coherence tomography angiography (OCTA) compared to iris fluorescein angiography (IFA). A 60-year-old man with a diagnosis of iris nevus in the left eye was referred to our department for IFA and iris OCTA. The iris vasculature in IFA was visible only in the early phases, but not clearly...
September 2016: Case Reports in Ophthalmology
https://www.readbyqxmd.com/read/27784235/study-in-treatment-of-collagen-induced-arthritis-in-dba-1-mice-model-by-genistein
#19
Peng Zhang, Yiping Hu, Jinchao Li, Ling Qin, Ye Yue, Wenxiang Cheng, Xiaohua Pan
BACKGROUND: This work aimed to evaluate the effects of genistein treatment in Collagen Induced Arthritis (CIA) mouse model. METHODS: CIA was elicited in DBA/1 Mice by an intradermal injection of 100 μL of an emulsion of bovine type II collagen (CII) in isovolumic incomplete Freund's adjuvant (IFA) at the base of the tail. Twenty-one days later, a second injection of CII in IFA was administered at the base of the tail. After the symptoms of arthritis showed in mouse model, we divided animals into two groups according to their clinical symptom scores...
October 25, 2016: Current Pharmaceutical Design
https://www.readbyqxmd.com/read/27778241/monitoring-toxic-ionic-liquids-in-zebrafish-danio-rerio-with-desorption-electrospray-ionization-mass-spectrometry-imaging-desi-msi
#20
Consuelo J Perez, Alessandra Tata, Michel L de Campos, Chun Peng, Demian R Ifa
Ambient mass spectrometry imaging has become an increasingly powerful technique for the direct analysis of biological tissues in the open environment with minimal sample preparation and fast analysis times. In this study, we introduce desorption electrospray ionization mass spectrometry imaging (DESI-MSI) as a novel, rapid, and sensitive approach to localize the accumulation of a mildly toxic ionic liquid (IL), AMMOENG 130 in zebrafish (Danio rerio). The work demonstrates that DESI-MSI has the potential to rapidly monitor the accumulation of IL pollutants in aquatic organisms...
October 24, 2016: Journal of the American Society for Mass Spectrometry
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