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https://www.readbyqxmd.com/read/28815296/autoantibodies-against-rods-and-rings-related-impdh2-in-hepatitis-c-genotype-1-and-daa-therapy-in-a-real-life-cohort
#1
Werner Dammermann, Susanne Polywka, Inga Dettmann, Swantje Mindorf, Lars Komorowski, Malte Wehmeyer, Julian Schulze Zur Wiesch, Winfried Stöcker, Stefan Lüth
Autoantibodies against inosine-5'-monophosphate-dehydrogenase-2 (IMPDH2; "rods and rings" pattern) develop in chronic hepatitis C (CHC) patients under treatment with peg-interferon (IFN) and ribavirin (RBV), an inhibitor of IMPDH2. We investigated the influence of the alternative therapy with direct-acting antivirals (DAA)/ribavirin on anti-IMPDH2 autoantibody generation and the use of anti-IMPDH2 development as a marker for therapy outcome (sustained virologic response, SVR). We analyzed a "real life" cohort of 104 unselected CHC genotype 1 (GT1) patients treated with IFN/first-generation DAA/RBV prospectively compared to a historic cohort of 59 IFN/RBV-treated CHC GT1 patients...
August 16, 2017: Medical Microbiology and Immunology
https://www.readbyqxmd.com/read/28799592/rapid-determination-of-the-tumour-stroma-ratio-in-squamous-cell-carcinomas-with-desorption-electrospray-ionization-mass-spectrometry-desi-ms-a-proof-of-concept-demonstration
#2
Michael Woolman, Alessandra Tata, Delaram Dara, Jalna Meens, Elisa D'Arcangelo, Consuelo J Perez, Shamina Saiyara Prova, Emma Bluemke, Howard J Ginsberg, Demian Ifa, Alison McGuigan, Laurie Ailles, Arash Zarrine-Afsar
Squamous cell carcinomas constitute a major class of head & neck cancers, where the tumour stroma ratio (TSR) carries prognostic information. Patients affected by stroma-rich tumours exhibit a poor prognosis and a higher chance of relapse. As such, there is a need for a technology platform that allows rapid determination of the tumour stroma ratio. In this work, we provide a proof-of-principle demonstration that Desorption Electrospray Ionization Mass Spectrometry (DESI-MS) can be used to determine tumour stroma ratios...
August 11, 2017: Analyst
https://www.readbyqxmd.com/read/28795800/prompt-and-robust-humoral-immunity-elicited-by-a-conjugated-chimeric-malaria-antigen-with-a-truncated-flagellin
#3
Fangxia Guo, Yong Dong Liu, Chun Zhang, Qi Wang, Lianyan Wang, Yuhui Gao, Jingxiu Bi, Heng Wang, Zhiguo Su
As one of the pathogen-associated molecular patterns (PAMPs), flagellin is recently utilized as a potent adjuvant for many subunit vaccines. In this study, a truncated flagellin (tFL) with deletion of the hypervariable regions was adopted as a carrier-adjuvant by chemical conjugation with a chimeric malaria antigen M.RCAg-1 (M312) via a hetero-bifunctional PEG linker. After boosting immunization in mice without any extra adjuvants, the M312-PEG-tFL conjugates elicited 100-1,000 times higher M312-specific antibody titers than M312, and 10-100 times higher than the physical mixture of M312 and tFL...
August 10, 2017: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/28794206/lipid-based-nutrient-supplements-during-pregnancy-and-lactation-did-not-affect-human-milk-oligosaccharides-and-bioactive-proteins-in-a-randomized-trial
#4
Josh M Jorgensen, Charles Arnold, Per Ashorn, Ulla Ashorn, David Chaima, Yin Bun Cheung, Jasmine Cc Davis, Yue-Mei Fan, Elisha Goonatilleke, Emma Kortekangas, Chiza Kumwenda, Carlito B Lebrilla, Kenneth Maleta, Sarah M Totten, Lauren D Wu, Kathryn G Dewey
Background: Human milk oligosaccharides (HMOs) and bioactive proteins are beneficial to infant health. Recent evidence suggests that maternal nutrition may affect the amount of HMOs and proteins in breast milk; however, the effect of nutrient supplementation on HMOs and bioactive proteins has not yet been well studied.Objective: We aimed to determine whether lipid-based nutrient supplements (LNSs) affect milk bioactive protein and HMO concentrations at 6 mo postpartum in women in rural Malawi. These are secondary outcomes of a previously published randomized controlled trial...
August 9, 2017: Journal of Nutrition
https://www.readbyqxmd.com/read/28793991/trial-of-ready-to-use-supplemental-food-and-corn-soy-blend-in-pregnant-malawian-women-with-moderate-malnutrition-a-randomized-controlled-clinical-trial
#5
Meghan Callaghan-Gillespie, Andrew A Schaffner, Patsy Garcia, Jocelyn Fry, Rachael Eckert, Shirin Malek, Indi Trehan, Chrissie Thakwalakwa, Kenneth M Maleta, Mark J Manary, Peggy C Papathakis
Background: Malnutrition during pregnancy in sub-Saharan Africa is associated with poor birth outcomes.Objective: This study compared maternal and offspring anthropometry for moderately malnourished pregnant women receiving ready-to-use supplemental food (RUSF), a fortified corn-soy blend (CSB+) with a daily multiple micronutrient antenatal supplement [United Nations International Multiple Micronutrient Preparation (UNIMMAP)], or standard of care comprising CSB+ and iron and folic acid (IFA).Design: A single-blind randomized controlled clinical trial was conducted in southern Malawi among 1828 pregnant women with moderate malnutrition, defined as a midupper arm circumference (MUAC) ≥20...
August 9, 2017: American Journal of Clinical Nutrition
https://www.readbyqxmd.com/read/28764157/seroepidemiology-of-spotted-fever-rickettsiosis-in-uttar-pradesh-a-prospective-study
#6
Chandra Dev Pati Tripathi, Mastan Singh, Jyotsna Agarwal, Chandra Kanta, Virendra Atam
INTRODUCTION: Spotted Fever Rickettsiosis (SFR), an acute febrile illness caused by Rickettsia rickettsii, R. conorii and R. akari which is associated with considerable morbidity and mortality. SFR is one of the most covert emerging infections of the present time which is prevalent in various parts of India as shown by the increase in the number of clinically diagnosed patients in various states except Uttar Pradesh. AIM: To diagnose SFR in clinically suspected patients using serological tests and recognition of common epidemiologic situations and clinical manifestations of SFR in the state of Uttar Pradesh...
June 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28761904/iglon5-antibody-neurological-accompaniments-and-outcomes-in-20-patients
#7
Josephe A Honorat, Lars Komorowski, Keith A Josephs, Kai Fechner, Erik K St Louis, Shannon R Hinson, Sabine Lederer, Neeraj Kumar, Avi Gadoth, Vanda A Lennon, Sean J Pittock, Andrew McKeon
OBJECTIVE: To describe the phenotypes, treatment response, and outcome of IgLON5 autoimmunity. METHODS: Archived serum and CSF specimens from 367 patients known to harbor unclassified antibodies which stained neural synapses diffusely (mimicking amphiphysin-IgG) were reevaluated by indirect immunofluorescence assay (IFA) using a composite of mouse tissues and recombinant IgLON5-transfected cell-based assay (CBA, Euroimmun). RESULTS: Available specimens (serum, 25; CSF, 9) from 26/367 patients (7%) had identical IFA appearance and robust IgLON5 CBA positivity...
September 2017: Neurology® Neuroimmunology & Neuroinflammation
https://www.readbyqxmd.com/read/28761480/detection-of-potentially-diagnostic-leishmania-antigens-with-western-blot-analysis-of-sera-from-patients-with-cutaneous-and-visceral-leishmaniases
#8
Seyyed Javad Seyyedtabaei, Ali Rostami, Ali Haghighi, Mehdi Mohebali, Bahram Kazemi, Shirzad Fallahi, Adel Spotin
BACKGROUND: Visceral leishmaniasis (VL) and cutaneous leishmaniasis (CL) are important public health problems in Iran. We aimed to evaluate the diagnostic potential of Western blot (WB) compared with indirect immunofluorescence test (IFAT) to serodiagnosis of leishmaniasis. METHODS: This study was performed from 2010-2014 and participants were different parts of Iran. Serum samples were obtained from 43 patients with proven CL, 33 patients with proven VL, 39 patients with other parasitic diseases and 23 healthy individuals...
April 2017: Iranian Journal of Parasitology
https://www.readbyqxmd.com/read/28757561/porcine-interferon-stimulated-gene-12a-restricts-porcine-reproductive-and-respiratory-syndrome-virus-replication-in-marc-145-cells
#9
Likai Ji, Xiang Zhou, Wan Liang, Jianjian Liu, Bang Liu
Porcine reproductive and respiratory syndrome virus (PRRSV) causes severe losses in the global pig industry. In the present study, we investigated the molecular characterization of porcine interferon stimulated gene 12a (ISG12A) and confirmed its anti-PRRSV ability for the first time. We found that porcine ISG12A was localized in mitochondria and significantly decreased the number of cells in G2/S phase. Porcine ISG12A mRNA was up-regulated in cells/tissues of Tongcheng (TC) pigs and Large White (LW) pigs after PRRSV challenge...
July 25, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28750668/from-hybridomas-to-a-robust-microalgal-based-production-platform-molecular-design-of-a-diatom-secreting-monoclonal-antibodies-directed-against-the-marburg-virus-nucleoprotein
#10
Franziska Hempel, Michael Maurer, Björn Brockmann, Christian Mayer, Nadine Biedenkopf, Anne Kelterbaum, Stephan Becker, Uwe G Maier
BACKGROUND: The ideal protein expression system should provide recombinant proteins in high quality and quantity involving low production costs only. However, especially for complex therapeutic proteins like monoclonal antibodies many challenges remain to meet this goal and up to now production of monoclonal antibodies is very costly and delicate. Particularly, emerging disease outbreaks like Ebola virus in Western Africa in 2014-2016 make it necessary to reevaluate existing production platforms and develop robust and cheap alternatives that are easy to handle...
July 27, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28749993/functional-characterization-of-an-aquaporin-from-a-microsporidium-nosema-bombycis
#11
Gong Chen, Wei Wang, Hongli Chen, Weijiang Dai, Xiangran Peng, Xiaoliang Li, Xudong Tang, Li Xu, Zhongyuan Shen
Microsporidia are a diverse group of eukaryotic organisms, capable of causing parasitic infections in both vertebrates and invertebrates. During the germination process, there is an increase in the osmotic pressure of microsporidian spores. As part of this study, we cloned a homologous aquaporin gene in Nosema bombycis, and named it Nosema bombycis aquaporin (NbAQP). Sequence analysis revealed that the NbAQP contains an open reading frame with a length of 750 bp and encodes a polypeptide of 249 amino acids...
2017: PloS One
https://www.readbyqxmd.com/read/28747311/an-n-terminal-pfs230-domain-produced-in-baculovirus-as-a-biological-active-transmission-blocking-vaccine-candidate
#12
Shwu-Maan Lee, Chia-Kuei Wu, Jordan L Plieskatt, Kazutoyo Miura, John M Hickey, C Richter King
Transmission-blocking vaccines have the potential to accelerate malaria parasite elimination by inducing antibodies that block parasite transmission from humans to mosquitoes. Pfs230, a gametocyte surface protein involved in gamete function, has long been a promising candidate. Due to the large size (3,135 amino acids), complex domains, and repeating six-cysteine (6-Cys) motifs with a multitude of disulfide bonds, the feasibility of expression of a full-length protein has been difficult. A priority focus, therefore, has been on the generation of single domains, including N-terminal fragments...
July 26, 2017: Clinical and Vaccine Immunology: CVI
https://www.readbyqxmd.com/read/28746450/anti-toxoplasma-gondii-antibodies-in-beef-cattle-slaughtered-in-the-metropolitan-region-of-bel%C3%A3-m-brazilian-amazon
#13
Ediclei Lima do Carmo, Rafaela Dos Anjos Pinheiro Bogoevich Morais, Michele de Souza Lima, Carla Cristina Guimarães de Moraes, George Rêgo Albuquerque, Aristeu Vieira da Silva, Marinete Marins Póvoa
The relevance of consuming raw or undercooked beef in the transmission of toxoplasmosis is unclear due to the high resistance of cattle to infection. However, this possibility needs to be considered in endemic areas, such as the Amazon, where the consumption of beef is frequent. The objective of this study was to determine the frequency of anti-Toxoplasma gondii IgG antibodies in beef cattle slaughtered in the metropolitan region of Belem, Pará state, Brazil. Blood samples were collected from 500 animals of both genders in a licensed slaughterhouse in Belém...
April 2017: Revista Brasileira de Parasitologia Veterinária, Brazilian Journal of Veterinary Parasitology
https://www.readbyqxmd.com/read/28744286/a-b-cell-driven-autoimmune-pathway-leading-to-pathological-hallmarks-of-progressive-multiple-sclerosis-in-the-marmoset-experimental-autoimmune-encephalomyelitis-model
#14
REVIEW
Bert A 't Hart, Jordon Dunham, Bart W Faber, Jon D Laman, Jack van Horssen, Jan Bauer, Yolanda S Kap
The absence of pathological hallmarks of progressive multiple sclerosis (MS) in commonly used rodent models of experimental autoimmune encephalomyelitis (EAE) hinders the development of adequate treatments for progressive disease. Work reviewed here shows that such hallmarks are present in the EAE model in marmoset monkeys (Callithrix jacchus). The minimal requirement for induction of progressive MS pathology is immunization with a synthetic peptide representing residues 34-56 from human myelin oligodendrocyte glycoprotein (MOG) formulated with a mineral oil [incomplete Freund's adjuvant (IFA)]...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28742401/chikungunya-virus-seroprevalence-and-associated-factors-among-hospital-attendees-in-two-states-of-southwest-nigeria-a-preliminary-assessment
#15
Olayinka M Olajiga, Olajumoke E Adesoye, Adewale P Emilolorun, Abiodun J Adeyemi, Emmanuel O Adeyefa, Ismail A Aderibigbe, Salmot A Adejumo, Wasiu O Adebimpe, Oluyinka O Opaleye, Waidi F Sule, Daniel O Oluwayelu
Chikungunya virus (CHIKV) is a re-emerging pathogen causing long-term polyarthritis and encephalitis. In conducting a preliminary investigation, we hypothesized that there is no serologic evidence of CHIKV infection among attendees of selected hospitals in Lagos and Osun States, Nigeria. Sera from 304 consecutively selected participants were screened for CHIKV IgG and IgM using ELISA. Findings were analyzed vis-à-vis participants' demographic and clinical data. Over 90.0% of the participants had never heard of CHIKV despite the fact that a large proportion of them (88...
August 2017: Immunological Investigations
https://www.readbyqxmd.com/read/28725312/two-cases-of-pneumatosis-cystoides-intestinalis-with-intraperitoneal-free-air
#16
Kensuke Wada, Nobuhiro Takeuchi, Masakazu Emori, Masanori Takada, Yusuke Nomura, Akihito Otsuka
Intraperitoneal free air (IFA) is sometimes accompanied by pneumatosis cystoides intestinalis (PCI); therefore, proper diagnosis is essential for PCI management. We report two cases of PCI with IFA. A 70-year-old female taking anti-psychotic medication for schizophrenia presented with repeated vomiting and high-grade fever. Computed tomography revealed small, linear gaseous cysts in the intestinal wall along with IFA. Although there was no sign of peritoneal irritation, intestinal perforation was not excluded...
June 2017: Gastroenterology Research
https://www.readbyqxmd.com/read/28724657/prenatal-lipid-based-nutrient-supplements-do-not-affect-pregnancy-or-childbirth-complications-or-cesarean-delivery-in-bangladesh-a-cluster-randomized-controlled-effectiveness-trial
#17
Malay K Mridha, Susana L Matias, Rina Rani Paul, Sohrab Hussain, Mostofa Sarker, Mokbul Hossain, Janet M Peerson, Stephen A Vosti, Kathryn G Dewey
Background: Pregnancy and childbirth complications and cesarean delivery are common in Bangladesh.Objective: We evaluated the effect of lipid-based nutrient supplements for pregnant and lactating women (LNS-PL) on pregnancy and childbirth complications and cesarean delivery.Methods: We conducted the Rang-Din Nutrition Study, a cluster-randomized controlled effectiveness trial within a community health program in rural Bangladesh. We enrolled 4011 pregnant women in early pregnancy. Women in 48 clusters received iron and folic acid (IFA; 60 mg Fe + 400 μg folic acid/d) and women in 16 clusters received LNS-PL (20 g/d, 118 kcal) containing essential fatty acids and 22 vitamins and minerals...
July 19, 2017: Journal of Nutrition
https://www.readbyqxmd.com/read/28720142/comparison-of-serological-assays-to-titrate-hantaan-and-seoul-hantavirus-specific-antibodies
#18
Weihong Li, Shouchun Cao, Quanfu Zhang, Jiandong Li, Shuo Zhang, Wei Wu, Jing Qu, Chuan Li, Mifang Liang, Dexin Li
BACKGROUND: Hantaan and Seoul viruses, in the Hantavirus genus, are known to cause hemorrhagic fever with renal syndrome (HFRS). The plaque reduction neutralization test (PRNT), as conventional neutralization test for hantaviruses, is laborious and time-consuming. Alternatives to PRNT for hantaviruses are required. METHODS: In this study, the methods for Hantaan and Seoul viruses serological typing including microneutralization test (MNT), pseudoparticle neutralization test (PPNT) and immunofluorescence assay based on viral glycoproteins (IFA-GP) were developed and compared with PRNT using a panel of 74 sera including 44 convalescent sera of laboratory confirmed HFRS patients and 30 patients sera of non-hantavirus infection...
July 18, 2017: Virology Journal
https://www.readbyqxmd.com/read/28718045/full-length-genome-sequence-analysis-of-four-subgroup-j-avian-leukosis-virus-strains-isolated-from-chickens-with-clinical-hemangioma
#19
Lulu Lin, Peikun Wang, Yongli Yang, Haijuan Li, Teng Huang, Ping Wei
Since 2014, cases of hemangioma associated with avian leukosis virus subgroup J (ALV-J) have been emerging in commercial chickens in Guangxi. In this study, four strains of the subgroup J avian leukosis virus (ALV-J), named GX14HG01, GX14HG04, GX14LT07, and GX14ZS14, were isolated from chickens with clinical hemangioma in 2014 by DF-1 cell culture and then identified with ELISA detection of ALV group specific antigen p27, the detection of subtype specific PCR and indirect immunofluorescence assay (IFA) with ALV-J specific monoclonal antibody...
July 17, 2017: Virus Genes
https://www.readbyqxmd.com/read/28715231/bayesian-estimation-for-item-factor-analysis-models-with-sparse-categorical-indicators
#20
Sierra A Bainter
Psychometric models for item-level data are broadly useful in psychology. A recurring issue for estimating item factor analysis (IFA) models is low-item endorsement (item sparseness), due to limited sample sizes or extreme items such as rare symptoms or behaviors. In this paper, I demonstrate that under conditions characterized by sparseness, currently available estimation methods, including maximum likelihood (ML), are likely to fail to converge or lead to extreme estimates and low empirical power. Bayesian estimation incorporating prior information is a promising alternative to ML estimation for IFA models with item sparseness...
July 17, 2017: Multivariate Behavioral Research
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