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https://www.readbyqxmd.com/read/29777291/growth-of-asymptomatic-intracranial-fusiform-aneurysms-incidence-and-risk-factors
#1
Jusun Moon, Young Dae Cho, Dong Hyun Yoo, Jeongjun Lee, Hyun-Seung Kang, Won-Sang Cho, Jeong Eun Kim, Li Zhang, Moon Hee Han
PURPOSE: Growth of intracranial fusiform aneurysms (IFA) may become clinically problematic through a mass effect or rupture. We investigated the growth rate and factors contributing to growth in asymptomatic untreated IFA. METHOD: As a retrospective review, we assessed patients diagnosed with asymptomatic IFA between August 2000 and September 2014, all untreated. No acute or symptomatic dissecting lesions were considered. Clinical and serial angiographic follow-up data were analyzed, defining growth as expansion > 2 mm in one or more dimensions...
May 18, 2018: Clinical Neuroradiology
https://www.readbyqxmd.com/read/29756651/optical-signature-of-nerve-tissue-exploratory-ex-vivo-study-comparing-optical-histological-and-molecular-characteristics-of-different-adipose-and-nerve-tissues
#2
Andrea J R Balthasar, Torre M Bydlon, Hans Ippel, Marjolein van der Voort, Benno H W Hendriks, Gerald W Lucassen, Geert-Jan van Geffen, Maarten van Kleef, Paul van Dijk, Arno Lataster
BACKGROUND: During several anesthesiological procedures, needles are inserted through the skin of a patient to target nerves. In most cases, the needle traverses several tissues-skin, subcutaneous adipose tissue, muscles, nerves, and blood vessels-to reach the target nerve. A clear identification of the target nerve can improve the success of the nerve block and reduce the rate of complications. This may be accomplished with diffuse reflectance spectroscopy (DRS) which can provide a quantitative measure of the tissue composition...
May 14, 2018: Lasers in Surgery and Medicine
https://www.readbyqxmd.com/read/29753281/a-network-pharmacology-integrated-metabolomics-strategy-for-clarifying-the-difference-between-effective-compounds-of-raw-and-processed-farfarae-flos-by-ultra-high-performance-liquid-chromatography-quadrupole-time-of-flight-mass-spectrometry
#3
Mingya Ding, Zhen Li, Xie-An Yu, Dong Zhang, Jin Li, Hui Wang, Jun He, Xiu-Mei Gao, Yan-Xu Chang
This study aimed to clarify the difference between the effective compounds of raw and processed Farfarae flos using a network pharmacology-integrated metabolomics strategy. First, metabolomics data were obtained by ultra high-performance liquid chromatography-quadrupole-time of flight mass spectrometry (UHPLC-Q-TOF/MS). Then, metabolomics analysis was developed to screen for the influential compounds that were different between raw and processed Farfarae flos. Finally, a network pharmacology approach was applied to verify the activity of the screened compounds...
May 4, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/29751360/intact-intraoperative-fluorescence-angiography-ifa-to-prevent-anastomotic-leak-in-rectal-cancer-surgery-a-randomised-controlled-trial
#4
G Armstrong, J Croft, N Corrigan, J M Brown, V Goh, P Quirke, C Hulme, D Tolan, A Kirby, R Cahill, R O'Connell, D Miskovic, M Coleman, D G Jayne
PURPOSE: Anastomotic leak (AL) is a major complication of rectal cancer surgery. Despite advances in surgical practice, rates of AL have remained static at around 10-15%. The aetiology of AL is multifactorial, but one of the most crucial risk factors, which is mostly under the control of the surgeon, is blood supply to the anastomosis. The MRC/NIHR IntAct study will determine whether assessment of anastomotic perfusion using a fluorescent dye (Indocyanine Green) and near-infrared laparoscopy can minimise the rate of anastomotic leak as compared to conventional white light laparoscopy...
May 11, 2018: Colorectal Disease: the Official Journal of the Association of Coloproctology of Great Britain and Ireland
https://www.readbyqxmd.com/read/29751298/role-of-cyclic-diguanylate-in-affecting-microbial-community-shifts-at-different-ph-during-the-operation-of-simultaneous-partial-nitrification-anammox-and-denitrification-process
#5
Chao Wang, Sitong Liu, Xiaochen Xu, Yongzhao Guo, Fenglin Yang, Dong Wang
The intracellular cyclic diguanylate acid (c-di-GMP) has emerged as a prominent second signal molecule that coordinates sessile-motile transition and biofilm formation in many bacteria. Herein, we study the role of c-di-GMP in affecting microbial community shifts at different pH levels during simultaneous partial nitrification, anammox and denitrification process (SNAD) in integrated fixed film activated sludge (IFAS) reactor. The results demonstrated that the contents of c-di-GMP notably decreased in suspended sludge, whereas the contents of c-di-GMP in biofilm had no significant change as pH gradually increased from 7...
May 8, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/29749366/experimental-zika-virus-infection-in-aedes-aegypti-susceptibility-transmission-co-infection-with-dengue-chikungunya-viruses
#6
Devendra T Mourya, Mangesh D Gokhale, Triparna D Majumdar, Pragya D Yadav, Vimal Kumar, Mangala S Mavale
Background & objectives: There are reports about the susceptibility of Aedes mosquitoes to ZIKV from various countries, however, no such information is available from Indian sub-continent, although, high level of group cross-reactivity of ZIKV with other flaviviruses has been reported. During outbreak situations, many cases of Dengue (DEN) and Chikungunya (CHIK) are reported. In such scenario, vector mosquitoes are likely to get co-infection/secondary-infection with one or other virus...
January 2018: Indian Journal of Medical Research
https://www.readbyqxmd.com/read/29743308/performance-evaluation-of-a-prototype-architect-antibody-assay-for-babesia-microti
#7
Kevin Cheng, Kelly E Coller, Christopher C Marohnic, Zachary A Pfeiffer, James R Fino, Randee R Elsing, Janet Bergsma, Marilee A Marcinkus, Alak K Kar, Orlando H Gumbs, Kathy S Otis, Jeffrey Fishpaugh, Phillip W Schultz, Mark R Pope, Alfredo R Narvaez, Susan J Wong, Susan Madison-Antenucci, Thomas P Leary, George J Dawson
The tick-borne protozoan Babesia microti is responsible for more than 200 cases of transfusion-transmitted babesiosis (TTB) infection in the United States over the last 30 years. Measures to mitigate the risk of TTB include nucleic acid testing (NAT) and B. microti antibody testing. A fully automated prototype B. microti antibody test was developed on the ARCHITECT instrument. The specificity was determined to be 99.98% in volunteer blood donors (n=28,740) from areas considered as low endemic for B. microti The sensitivity of the prototype test was studied in experimentally-infected macaques; a total of 128 samples were detected compared to 125 with the indirect fluorescent antibody test (IFA), additionally, 83 (89...
May 9, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29737440/detection-of-toxoplasma-specific-immunoglobulin-g-in-human-sera-performance-comparison-of-in-house-dot-elisa-with-eclia-and-elisa
#8
Aref Teimouri, Mohammad Hossein Modarressi, Saeedeh Shojaee, Mehdi Mohebali, Nima Zouei, Mostafa Rezaian, Hossein Keshavarz
In the current study, performance of electrochemiluminescence immunoassay (ECLIA) in detection of anti-toxoplasma IgG in human sera was compared with that of enzyme-linked immunosorbent assay (ELISA). Furthermore, performance of an in house Dot-ELISA in detection of anti-toxoplasma IgG was compared with that of ECLIA and ELISA. In total, 219 human sera were tested to detect anti-toxoplasma IgG using Dynex DS2® and Roche Cobas® e411 Automated Analyzers. Discordant results rechecked using immunofluorescence assay (IFA)...
May 8, 2018: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/29736821/monoclonal-antibody-against-the-universal-m2-epitope-of-influenza-a-virus
#9
Mingfang Feng, Zhuangchuan Yuan, Wenjun Xia, Xiaozhi Huang, XingBo Wang, Yan Yan, Min Liao, Jiyong Zhou
M2 protein, a highly conserved protein of influenza A virus (IAV), plays an important role in virus particle uncoating, assembly, and budding. In the present study, eight monoclonal antibodies (mAbs) against the M2 protein of the H3N2 IAV strain were generated with recombinant truncated M2 protein or BSA-coupled M2 peptides as immunogens. The linear epitopes recognized by the mAbs were defined by IFA and peptide ELISA. The results showed that mAb 10F4 recognized an epitope located in the N-terminal 6-12 amino acids of the M2 peptide, and the mAbs 10D9, 1E2, 4B5, and 5G10 recognized the epitopes located in the C-terminal 62-77 amino acids of the M2 peptide...
May 7, 2018: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29735837/diagnostic-performance-of-serological-tests-to-detect-antibodies-against-acute-scrub-typhus-infection-in-central-india
#10
Kiran Pote, Rahul Narang, Pradeep Deshmukh
Background: Differentiating scrub typhus from other acute febrile illness is difficult due to non specificity of clinical symptoms and relative absence of eschar in Indian population. The diagnosis thus relies mainly on laboratory tests. Antibody based serological tests are mainstay of scrub typhus diagnosis. Here, we evaluated the diagnostic performance of IgM ELISA, IgM IFA and ICT to detect antibodies against O. tsutsugamushi in acute serum of febrile patients. Methodology: The serum samples from 600 randomly selected patients suffering from acute undifferentiated fever were tested by all the three tests mentioned above...
January 2018: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/29734823/from-the-field-improving-fetal-and-infant-growth-in-vulnerable-populations
#11
Seth Adu-Afarwuah
BACKGROUND: This article summarizes a presentation given at the 2017 Dairy for Global Nutrition Conference in Boise, Idaho. OBJECTIVE: To give an overview of the pattern of early growth faltering in developing countries and examine the implications of the iLiNS-DYAD randomized trial in Ghana. METHODS: The pattern of growth faltering in developing countries was outlined. In Ghana, 1320 women ≤ 20 weeks of pregnancy were assigned to 20 g/d small-quantity lipid-based nutrient supplement (SQ-LNS; LNS group) or multiple micronutrients (MMNs) containing 22 and 18 vitamins and minerals, respectively, until 6 months postpartum, or iron and folic acid (IFA) until delivery, and thereafter placebo until 6 months postpartum...
January 1, 2018: Food and Nutrition Bulletin
https://www.readbyqxmd.com/read/29720430/evaluation-of-a-rapid-diagnostic-test-for-the-detection-of-burkholderia-pseudomallei-in-the-lao-people-s-democratic-republic
#12
Kate L Woods, Latsaniphone Boutthasavong, Caoimhe NicFhogartaigh, Sue J Lee, Viengmon Davong, David P AuCoin, David A B Dance
Burkholderia pseudomallei causes significant global morbidity and mortality, with the highest disease burden in parts of Asia where culture-based diagnosis is often not available. We prospectively evaluated the Active Melioidosis Detect (AMD, InBios International, USA) lateral flow immunoassay (LFI) for rapid detection of B. pseudomallei in turbid blood cultures, pus, sputum, sterile fluid, urine, and sera. Performance was compared to B. pseudomallei detection using monoclonal antibody latex agglutination (LA) and immunofluorescence assays (IFA), with culture as the gold standard...
May 2, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29720135/compliance-with-iron-and-folic-acid-supplementation-ifas-and-associated-factors-among-pregnant-women-results-from-a-cross-sectional-study-in-kiambu-county-kenya
#13
Mary Wanjira Kamau, Waithira Mirie, Samuel Kimani
BACKGROUND: Macro and micronutrients including iron and folic acid deficiencies are prevalent in Kenya, particularly during pregnancy resulting in anaemia. Despite efforts to control anaemia in pregnancy by adopting Iron and Folic Acid Supplementation (IFAS), this public health problem has persisted contributing to significant morbidity and mortality. The problem notwithstanding, there is poor IFAS compliance, whose reasons remain poorly understood, calling for their investigations. We sought to determine compliance status with IFAS and associated factors among pregnant women...
May 2, 2018: BMC Public Health
https://www.readbyqxmd.com/read/29706479/scrub-typhus-diagnosis-on-acute-specimens-using-serological-and-molecular-assays-a-3-year-prospective-study
#14
Munegowda Koralur, Rahul Singh, Muralidhar Varma, Shalini Shenoy, Vasudeva Acharya, Asha Kamath, John Stenos, Eugen Athan, Indira Bairy
Scrub typhus (ST) is an underdiagnosed acute febrile illness in the Asia Pacific region with recent reemergence. Clinical diagnosis is difficult, and laboratory confirmation is largely based on serological and molecular tests. However, Weil-Felix test still remains the only test available in much of the rural tropics where a disproportionate number of cases occur. Sensitive and affordable assays are important for broader use and accurate diagnosis. We evaluated the diagnostic capabilities of serological and molecular assays on single acute clinical samples...
February 24, 2018: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/29703230/golgi-tethering-factor-golgin-97-suppresses-breast-cancer-cell-invasiveness-by-modulating-nf-%C3%AE%C2%BAb-activity
#15
Rae-Mann Hsu, Cai-Yan Zhong, Chih-Liang Wang, Wei-Chao Liao, Chi Yang, Shih-Yu Lin, Jia-Wei Lin, Hsiao-Yun Cheng, Po-Yu Li, Chia-Jung Yu
BACKGROUND: Golgin-97 is a tethering factor in the trans-Golgi network (TGN) and is crucial for vesicular trafficking and maintaining cell polarity. However, the significance of golgin-97 in human diseases such as cancer remains unclear. METHODS: We searched for a potential role of golgin-97 in cancers using Kaplan-Meier Plotter ( http://kmplot.com ) and Oncomine ( www.oncomine.org ) datasets. Specific functions of golgin-97 in migration and invasion were examined in golgin-97-knockdown and golgin-97-overexpressing cells...
April 27, 2018: Cell Communication and Signaling: CCS
https://www.readbyqxmd.com/read/29698560/review-and-perspectives-on-the-applications-of-mass-spectrometry-imaging-under-ambient-conditions
#16
Consuelo J Perez, Aafreen K Bagga, Shamina S Prova, Maryam Y Taemeh, Demian R Ifa
Ambient MS-based techniques are performed under ambient conditions in which the ionization and desorption occur in the open environment allowing the direct analysis of molecules on surfaces with minimal or no sample preparation. A selected group of ambient MS techniques demonstrate imaging capabilities that can provide information about the localization of molecules on complex sample surfaces such as biological tissues. 2D, 3D, and multimodal imaging have unlocked an array of applications to systematically address complex problems in many areas of research such as drug monitoring, natural products forensics, and cancer diagnostics...
April 26, 2018: Rapid Communications in Mass Spectrometry: RCM
https://www.readbyqxmd.com/read/29698128/preparation-of-the-secretory-recombinant-alv-j-gp85-protein-using-pichia-pastoris-and-its-immunoprotection-as-vaccine-antigen-combining-with-cpg-odn-adjuvant
#17
Weifang Jing, Jinrun Zhou, Chunyang Wang, Jianhua Qiu, Huijun Guo, Hongmei Li
This study focuses on preparing the secretory recombinant J subgroup of avian leukosis virus (ALV-J) gp85 protein using Pichia pastoris and evaluating its immunoprotection as vaccine antigen combining with CpG-ODN adjuvant. The secretory recombinant plasmid pPIC9-gp85 containing ALV-J gp85 gene was designed and was transfected into the genome of P. pastoris (GS115) cells. The recombinant plasmid was expressed under the induction of methanol. The expressed products in the medium of the cells were purified and identified with endoglycosidase digestion assay and western blot mediated with monoclonal antibody (MAb) JE9...
April 26, 2018: Viral Immunology
https://www.readbyqxmd.com/read/29687068/a-case-of-pediatric-keratosis-lichenoides-chronica-with-unusual-presentation-of-severe-oral-erosions
#18
Maryam Nasimi, Arghavan Azizpour, Vahideh Lajevardi, Azita Nikoo, Ifa Etesami
No abstract text is available yet for this article.
April 2018: JAAD Case Reports
https://www.readbyqxmd.com/read/29684605/development-of-monoclonal-antibodies-against-igm-of-sea-bass-lateolabrax-japonicus-and-analysis-of-phagocytosis-by-migm-lymphocytes
#19
Shun Yang, Xiaoqian Tang, Xiuzhen Sheng, Jing Xing, Wenbin Zhan
B cells in some fish were recently found to have potent phagocytic activities. Sea bass (Lateolabrax japonicus) as an important economical marine fish species, it could be used as an appropriate model to study the functions of B cells in phagocytosis. In the paper, three positive hybridomas designated as 1E11, 2H4 and 3F3 secreting monoclonal antibodies (MAbs) against sea bass immunoglobulin M (IgM) were produced and used as research tools. Indirect enzyme-linked immunosorbent assay showed that all the three MAbs had a high binding capacity with sea bass serum IgM...
April 20, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29683723/model-fit-and-item-factor-analysis-overfactoring-underfactoring-and-a-program-to-guide-interpretation
#20
D Angus Clark, Ryan P Bowles
In exploratory item factor analysis (IFA), researchers may use model fit statistics and commonly invoked fit thresholds to help determine the dimensionality of an assessment. However, these indices and thresholds may mislead as they were developed in a confirmatory framework for models with continuous, not categorical, indicators. The present study used Monte Carlo simulation methods to investigate the ability of popular model fit statistics (chi-square, root mean square error of approximation, the comparative fit index, and the Tucker-Lewis index) and their standard cutoff values to detect the optimal number of latent dimensions underlying sets of dichotomous items...
April 23, 2018: Multivariate Behavioral Research
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