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Yu Chun Cai, Fen Wu, Wei Hu, Jiaxu Chen, Shao Hong Chen, Bin Xu, Yan Lu, Lin Ai, Chun Li Yang, Shimin Zhao
Babesiosis has become a new global threat impacting human health, and most human babesiosis cases are caused by Babesia microti. Until now few antigens of B. microti have been described which can be used for the diagnosis of human babesiosis. In the present study, we report on the bioinformatic analysis, cloning and expression of the sequence encoding the B. microti seroreactive antigen 5-1-1 to investigate its potential incorporation in serologic diagnostic tools for babesiosis. Bioinformatic analysis and recombinant gene expression were performed to molecularly characterize seroreactive antigen 5-1-1...
March 17, 2018: Acta Tropica
Zhancheng Tian, Xiaoyue Du, Junzheng Du, Shandian Gao, Ruiming Yu, Muhammad Adeel Hassan, Guangyuan Liu, Jianxun Luo, Hong Yin
Tropical theileriosis, caused by Theileria annulata, is distributed worldwide and causes great economic losses in dairy. The reliable diagnostic method is critical for prevention and control of the disease. In this study, a sporozoite and macroschizont gene 2 (spm2) protein from T. annulata was used to develop an indirect ELISA for tropical theileriosis. Specificity test showed that there were no cross-reactions with antibodies raised against other bovine piroplasm species using ELISA or western blotting...
March 12, 2018: Acta Tropica
Simon Bengtsson, Mark de Blois, Britt-Marie Wilén, David Gustavsson
The aerobic granular sludge (AGS) technology is growing towards becoming a mature option for new municipal wastewater treatment plants and capacity extensions. A process based on AGS was compared to conventional activated sludge processes (with and without enhanced biological phosphorus removal), an integrated fixed-film activated sludge (IFAS) process and a membrane bioreactor (MBR) by estimating the land area demand (foot-print), electricity demand and chemicals consumption. The process alternatives compared included pre-settling, sludge digestion and necessary post-treatment to achieve effluent concentrations of 8 mg/L nitrogen and 0...
March 13, 2018: Environmental Technology
Wenyou Hu, Huifeng Wang, Lurui Dong, Biao Huang, Ole K Borggaard, Hans Christian Bruun Hansen, Yue He, Peter E Holm
Intensive human activities, in particular agricultural and industrial production have led to heavy metal accumulation in the peri-urban agricultural soils of China threatening soil environmental quality and agricultural product security. A combination of spatial analysis (SA), Pb isotope ratio analysis (IRA), input fluxes analysis (IFA), and positive matrix factorization (PMF) model was successfully used to assess the status and sources of heavy metals in typical peri-urban agricultural soils from a rapidly developing region of China...
March 9, 2018: Environmental Pollution
Raghavendra Yadavalli, Tobili Y Sam-Yellowe
In this study we performed light, immunofluorescent and transmission electron microscopy of Colpodella trophozoites to characterize trophozoite morphology and protein distribution. The use of Giemsa staining and antibodies to distinguish Colpodella life cycle stages has not been performed previously. Rhoptry and β-tubulin antibodies were used in immunofluorescent assays (IFA) to identify protein localization and distribution in the trophozoite stage of Colpodella (ATCC 50594). We report novel data identifying "doughnut-shaped" vesicles in the cytoplasm and apical end of Colpodella trophozoites reactive with antibodies specific to Plasmodium merozoite rhoptry proteins...
December 2017: International Microbiology: the Official Journal of the Spanish Society for Microbiology
Sophiya Dulal, Frédérique Liégeois, David Osrin, Adam Kuczynski, Dharma S Manandhar, Bhim P Shrestha, Aman Sen, Naomi Saville, Delan Devakumar, Audrey Prost
Introduction: Multiple Micronutrient (MMN) supplementation during pregnancy can decrease the proportion of infants born low birth weight and small for gestational age. Supplementation could also enhance children's cognitive function by improving access to key nutrients during fetal brain development and increasing birth weight, especially in areas where undernutrition is common. We tested the hypothesis that children whose mothers received MMN supplementation during pregnancy would have higher intelligence in early adolescence compared with those receiving Iron and Folic Acid (IFA) only...
2018: BMJ Global Health
Aramsri Meeprom, Catherine B Chan, Weerachat Sompong, Sirichai Adisakwattana
Methylglyoxal (MG) is a reactive precursor to advanced glycation end-products (AGEs), which exert deleterious effects on cells and tissues. MG also causes pancreatic β-cell dysfunction and apoptosis. Isoferulic acid (IFA), a naturally occurring cinnamic acid derivative, is considered to be an antiglycating agent. However, the effect of IFA on MG-induced pancreatic β-cell dysfunction remains unknown. The objective of this study was to determine the protective effect of IFA against MG-induced mitochrondrial dysfunction and apoptosis in INS-1 pancreatic β-cells...
March 8, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Nan Lv, Haishuang Tang, Shiyue Chen, Xinrui Wang, Yibin Fang, Christof Karmonik, Qinghai Huang, Jianmin Liu
OBJECTIVE: Vessel wall magnetic resonance imaging (MRI) has been suggested as a potential in vivo method to detect inflammation of aneurysm wall and identify unruptured intracranial aneurysm (UIA) with high rupture risk. This study aims to investigate the correlation between aneurysm wall enhancement (AWE) on vessel wall MRI and rupture-related morphological parameters in patients with multiple UIAs. METHODS: Clinical data and VW-MRI images were reviewed in 14 patients with 30 multiple UIAs...
March 7, 2018: World Neurosurgery
Yusuke Sayama, Aya Zamoto-Niikura, Chieko Matsumoto, Masayuki Saijo, Chiaki Ishihara, Keiji Matsubayashi, Tadashi Nagai, Masahiro Satake
BACKGROUND: Human babesiosis is caused mainly by Babesia microti and has recently become a public health concern due to an increase in transfusion-transmitted infection. Thus, the development of an antibody detection method with high specificity and sensitivity is a priority. Seroreactivity against B. microti has been reported to be highly specific not only to B. microti lineages but also to sublineages. This study aimed to elucidate the human antibody reactivity against various lineages, including US, Kobe, and Hobetsu, and sublineages (North America and East Asia) in the US lineage...
March 9, 2018: Transfusion
Ai-Ling Tian, MingMin Lu, Guillermo Calderón-Mantilla, Evangelia Petsalaki, Tania Dottorini, XiaoWei Tian, YuJian Wang, Si-Yang Huang, Jun-Ling Hou, XiangRui Li, Hany M Elsheikha, Xing-Quan Zhu
BACKGROUND: The molecular structure of Fasciola gigantica 14-3-3 protein has been characterized. However, the involvement of this protein in parasite pathogenesis remains elusive and its effect on the functions of innate immune cells is unknown. We report on the cloning and expression of a recombinant F. gigantica 14-3-3 epsilon protein (rFg14-3-3e), and testing its effects on specific functions of goat peripheral blood mononuclear cells (PBMCs). METHODS: rFg14-3-3e protein was expressed in Pichia pastoris...
March 6, 2018: Parasites & Vectors
Ji Eun Yu, In-Ohk Ouh, Hyeonjeong Kang, Hye-Young Lee, Kwang-Myun Cheong, In-Soo Cho, Sang-Ho Cha
Porcine reproductive and respiratory syndrome (PRRS) has been proposed to be one of the most important infectious diseases causing serious economic loss in swine industry worldwide. Due to its increasing genetic diversity, a rapid and accurate diagnosis is critical in PRRS control. The immunochromatographic strip test (ICST) has been recognized as a type of immunoassay with speed and convenience. In this study, the on-site diagnostic method with immunochromatographic assay was developed for detection of PRRSV-specific antibodies based on colloidal gold nanoparticles-labeled dual-type nucleocapsid (N) proteins encoded by open reading frame (ORF) 7, and evaluated using 991 field samples from pig farms and 66 serum samples from experimentally PRRSV-inoculated pigs...
March 6, 2018: Journal of Veterinary Science
Zewdie Birhanu, Gina M Chapleau, Stephanie E Ortolano, Girma Mamo, Stephanie L Martin, Katherine L Dickin
After decades of global response to iron-deficiency anemia, lessons learned from antenatal iron-folic acid (IFA) supplementation can inform new micronutrient supplementation efforts. The World Health Organization recommends calcium supplementation for the prevention of preeclampsia; however, little is documented on how to design programs to integrate calcium into the standard of care. Twenty interviews with pregnant women and 22 interviews with health providers and volunteers in two districts in Ethiopia were conducted to examine how barriers and facilitators to antenatal care, IFA supplementation, and initial reactions to calcium supplements and regimen might influence adherence and inform future programs...
February 2018: Maternal & Child Nutrition
Moshood O Omotayo, Katherine L Dickin, David L Pelletier, Stephanie L Martin, Jacqueline K Kung'u, Rebecca J Stoltzfus
Calcium (Ca) supplementation to prevent preeclampsia can save maternal and newborn lives, but there are no program models for integration into existing antenatal care platforms. We used a program impact pathway model to guide the design of integrated Ca and iron-folate (IFA) supplementation in Kenya. We provided healthcare providers with job aids (posters and counseling cards), trained them on counseling techniques and supplementation guidelines, and developed behavior change materials for pregnant women (pill-taking calendars)...
February 2018: Maternal & Child Nutrition
Khadija Begum, Césaire T Ouédraogo, K Ryan Wessells, Rebecca R Young, M Thierno Faye, Sara E Wuehler, Sonja Y Hess
The World Health Organization recommends iron and folic acid (IFA) supplementation for pregnant women. The high prevalence of anaemia among pregnant women in Niger warrants better understanding of the utilization of antenatal care (ANC) and IFA. We aimed to assess the prevalence of and factors associated with ANC coverage and adherence to IFA recommendation among pregnant women. Pregnant women (n = 923) from 64 randomly selected villages within the catchment area of 12 health centres were interviewed during a baseline household survey in Zinder, Niger...
February 2018: Maternal & Child Nutrition
Yared Hailemichael, Amber Woods, Tihui Fu, Qiuming He, Michael C Nielsen, Farah Hasan, Jason Roszik, Zhilan Xiao, Christina Vianden, Hiep Khong, Manisha Singh, Meenu Sharma, Faisal Faak, Derek Moore, Zhimin Dai, Scott M Anthony, Kimberly S Schluns, Padmanee Sharma, Victor H Engelhard, Willem W Overwijk
Anticancer vaccination is a promising approach to increase the efficacy of cytotoxic T lymphocyte-associated protein 4 (CTLA-4) and programmed death ligand 1 (PD-L1) checkpoint blockade therapies. However, the landmark FDA registration trial for anti-CTLA-4 therapy (ipilimumab) revealed a complete lack of benefit of adding vaccination with gp100 peptide formulated in incomplete Freund's adjuvant (IFA). Here, using a mouse model of melanoma, we found that gp100 vaccination induced gp100-specific effector T cells (Teffs), which dominantly forced trafficking of anti-CTLA-4-induced, non-gp100-specific Teffs away from the tumor, reducing tumor control...
February 26, 2018: Journal of Clinical Investigation
S John Calise, Bing Zheng, Tomoko Hasegawa, Minoru Satoh, Natasa Isailovic, Angela Ceribelli, Luis E C Andrade, Katherine Boylan, Ilaria Cavazzana, Marvin J Fritzler, Ignacio Garcia de la Torre, Falk Hiepe, Kathryn Kohl, Carlo Selmi, Yehuda Shoenfeld, Angela Tincani, Edward K L Chan
BACKGROUND: Anti-mitochondrial antibodies (AMA) are found in >90% of primary biliary cholangitis patients. Anti-rods/rings antibodies (anti-RR) are most commonly associated with interferon-α and ribavirin treatment in hepatitis C patients. Clinical laboratories routinely screen for AMA and anti-RR using indirect immunofluorescence on HEp-2 cells (HEp-2-IFA). Therefore, we sought to establish reference materials for use in AMA and anti-RR testing. METHODS: AMA-positive and anti-RR-positive human plasma samples (AMA-REF and RR-REF), identified as potential reference materials based on preliminary data, were further validated by multiple laboratories using HEp-2-IFA, immunoprecipitation (IP), western blotting, IP-western, line immunoassay (LIA), addressable laser bead immunoassay (ALBIA) and enzyme-linked immunosorbent assay (ELISA)...
February 26, 2018: Clinical Chemistry and Laboratory Medicine: CCLM
Na Liu, ChiYung Yam
As an alternative to methylammonium lead triiodide (MAPbI3 ), formamidinium lead triiodide (FAPbI3 ) perovskites have recently attracted significant attention because of their higher stability and smaller band gaps. Here, based on first-principles calculations, we investigate systematically the intrinsic defects in FAPbI3 . While methylammonium (MA)-related defects MAI and IMA in MAPbI3 have high formation energies, we found that formamidinium (FA)-related defects VFA , FAI and IFA in FAPbI3 have much lower formation energies...
February 23, 2018: Physical Chemistry Chemical Physics: PCCP
Ying Yu, Ying Chen, Yang Wang, Yuan Li, Lin Zhang, Jiuqing Xin
Mycoplasma gallisepticum (M. gallisepticum) is one of the most important pathogens that cause chronic respiratory disease in chickens. M. gallisepticum-derived lipid-associated membrane proteins (LAMPs) are thought to be one of the major factors in mycoplasma pathogenesis and are potent inducers of the host innate immune response. However, the interaction of pathogenic M. gallisepticum-derived LAMPs with Toll-like receptors (TLRs) and the signaling pathways responsible for activating inflammation and NF-κB have not been fully elucidated...
February 19, 2018: Microbial Pathogenesis
Arghavan Azizpour, Alireza Ghanadan, Maryam Nasimi, Ifa Etesami
BACKGROUND: Mycosis fungoides (MF) typically presents as erythematous scaly patches or plaques that may progress to cutaneous tumors. Although MF may be presented like other dermatoses, initial presentation as psoriasiform plaques simulating psoriasis is rare. Differentiating MF from psoriasis is important because systemic therapies used for psoriasis can worsen MF. We describe a case of psoriasiform MF and we also review the clinicopathological features of similar cases in the literature...
September 15, 2017: Dermatology Online Journal
Claudio Zett, Deborah M Rosa Stina, Renata Tiemi Kato, Eduardo Amorim Novais, Norma Allemann
PURPOSE: The aim of this study is to perform imaging of irises of different colors using spectral domain anterior segment optical coherence tomography angiography (AS-OCTA) and iris fluorescein angiography (IFA) and compare their effectiveness in examining iris vasculature. METHODS: This is a cross-sectional observational clinical study. Patients with no vascular iris alterations and different pigmentation levels were recruited. Participants were imaged using OCTA adapted with an anterior segment lens and IFA with a confocal scanning laser ophthalmoscope (cSLO) adapted with an anterior segment lens...
February 21, 2018: Graefe's Archive for Clinical and Experimental Ophthalmology
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