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https://www.readbyqxmd.com/read/28340426/porcine-parvovirus-induces-activation-of-nf-%C3%AE%C2%BAb-signaling-pathways-in-pk-15-cells-mediated-by-toll-like-receptors
#1
Liyan Cao, Jianfei Chen, Yanwu Wei, Hongyan Shi, Xin Zhang, Jing Yuan, Da Shi, Jianbo Liu, Xiangdong Zhu, Xin Wang, Shangjin Cui, Li Feng
Porcine parvovirus (PPV) is a pathogenic factor that primarily induces severe reproductive failure of pregnant swine, which results in extensive losses to the swine industry worldwide. In this study, a potential mechanism of PPV-induced activation of the nuclear transcription factor-kappaB (NF-κB) by infection in porcine kidney cells (PK-15) was elucidated for the first time. The subcellular localization of p65 analyzed by immunofluorescence assay (IFA) showed that PPV infection induced p65 translocation from the cytoplasm to the nucleus...
March 21, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/28333859/multicenter-prospective-study-for-laboratory-diagnosis-of-hhv8-infection-in-solid-organ-donors-and-transplant-recipients-and-evaluation-of-the-clinical-impact-after-transplantation
#2
Angela Chiereghin, Patrizia Barozzi, Evangelia Petrisli, Giulia Piccirilli, Liliana Gabrielli, Giovanni Riva, Leonardo Potenza, Gianni Cappelli, Nicola De Ruvo, Irene Libri, Umberto Maggiore, Maria Cristina Morelli, Luciano Potena, Paola Todeschini, Dino Gibertoni, Manuel Labanti, Gabriela Sangiorgi, Gaetano La Manna, Antonio Daniele Pinna, Mario Luppi, Tiziana Lazzarotto
BACKGROUND: We performed serological and molecular pretransplant screening in solid organ transplant (SOT) donors and recipients in north-central Italy and a surveillance program for human herpes virus 8 (HHV8) infection after transplant, aiming to establish an optimal management of HHV8 infection in SOT recipients. METHODS: For pretransplant HHV8 screening in both donors and recipients, 6 serological (4 indirect immunofluorescent assays (IFA) and 2 enzyme-linked immunosorbent assays (ELISA) - both HHV8 lytic and latent antigen-based) and 2 molecular assays were used...
March 22, 2017: Transplantation
https://www.readbyqxmd.com/read/28332274/time-course-expression-qtl-atlas-of-the-global-transcriptional-response-of-wheat-to-fusarium-graminearum
#3
Mina Samad-Zamini, Wolfgang Schweiger, Thomas Nussbaumer, Klaus F X Mayer, Hermann Buerstmayr
Fusarium head blight is a devastating disease of small grain cereals such as bread wheat (Triticum aestivum). The pathogen switches from a biotrophic to a nectrotrophic lifestyle in course of disease development forcing its host to adapt its defence strategies. Using a genetical genomics approach we illustrate genome-wide reconfigurations of genetic control over transcript abundances between two decisive time points after inoculation with the causative pathogen Fusarium graminearum. Whole transcriptome measurements have been recorded for 163 lines of a wheat doubled haploid population segregating for several resistance genes yielding 15 552 at 30 hours and 15 888 eQTL at 50 hours after inoculation...
March 23, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28297162/issue-information-ifa
#4
(no author information available yet)
No abstract text is available yet for this article.
March 2017: Journal of Hepato-biliary-pancreatic Sciences
https://www.readbyqxmd.com/read/28286760/the-effect-of-different-attentional-focus-instructions-during-finger-movement-tasks-in-healthy-subjects-an-exploratory-study
#5
Giacomo Rossettini, Marco Testa, Marco Vicentini, Paolo Manganotti
External focus of attention (EFA) and internal focus of attention (IFA) represent commonly used strategies to instruct subjects during exercise. Several studies showed EFA to be more effective than IFA to improve motor performance and learning. To date the role of these strategies on motor performance during finger movement was less studied. The objective of the study was to investigate motor performance, patient's preference induced by IFA and EFA, and the focus during control condition. Ten healthy right-handed participants performed a finger movement task in control, EFA, and IFA conditions (counterbalanced)...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28275128/home-fortification-during-the-first-1000-d-improves-child-development-in-bangladesh-a-cluster-randomized-effectiveness-trial
#6
Susana L Matias, Malay K Mridha, Fahmida Tofail, Charles D Arnold, Md Showkat A Khan, Zakia Siddiqui, Md Barkat Ullah, Kathryn G Dewey
Background: Nutrition during the first 1000 d is critical for brain development.Objective: We evaluated the effects on child development of home fortification with lipid-based nutrient supplements (LNSs) for mothers and/or children or micronutrient powder (MNP) for children.Design: We conducted a cluster-randomized effectiveness trial with 4 arms: 1) LNSs during pregnancy and the first 6 mo postpartum and LNSs for the offspring from 6 to 24 mo (LNS-LNS), 2) iron and folic acid (IFA) during pregnancy and the first 3 mo postpartum and LNSs for the children from 6 to 24 mo (IFA-LNS), 3) IFA (as above) and MNP for the offspring from 6 to 24 mo (IFA-MNP), and 4) IFA (as above) and no child supplement (IFA-Control)...
March 8, 2017: American Journal of Clinical Nutrition
https://www.readbyqxmd.com/read/28275125/lipid-based-nutrient-supplementation-in-the-first-1000-d-improves-child-growth-in-bangladesh-a-cluster-randomized-effectiveness-trial
#7
Kathryn G Dewey, Malay K Mridha, Susana L Matias, Charles D Arnold, Joseph R Cummins, Md Showkat Ali Khan, Zeina Maalouf-Manasseh, Zakia Siddiqui, Md Barkat Ullah, Stephen A Vosti
Background: Stunting in linear growth occurs mainly during the first 1000 d, from conception through 24 mo of age. Despite the recognition of this critical period, there have been few evaluations of the growth impact of interventions that cover most of this window.Objective: We evaluated home fortification approaches for preventing maternal and child undernutrition within a community-based health program. We hypothesized that small-quantity lipid-based nutrient supplements (LNSs) provided to women during pregnancy and the first 6 mo postpartum, LNSs provided to their offspring from 6 to 24 mo of age, or both would result in greater child length-for-age z score (LAZ) at 24 mo than iron and folic acid (IFA) provided to women during pregnancy and postpartum plus micronutrient powder (MNP) or no supplementation for their offspring from 6 to 24 mo...
March 8, 2017: American Journal of Clinical Nutrition
https://www.readbyqxmd.com/read/28275100/maternal-supplementation-with-small-quantity-lipid-based-nutrient-supplements-compared-with-multiple-micronutrients-but-not-with-iron-and-folic-acid-reduces-the-prevalence-of-low-gestational-weight-gain-in-semi-urban-ghana-a-randomized-controlled-trial
#8
Seth Adu-Afarwuah, Anna Lartey, Harriet Okronipa, Per Ashorn, Ulla Ashorn, Mamane Zeilani, Mary Arimond, Stephen A Vosti, Kathryn G Dewey
Background: It is unclear whether maternal supplementation with small-quantity lipid-based nutrient supplements (SQ-LNSs; 118 kcal/d) affects maternal weight.Objective: We compared several secondary anthropometric measures between 3 groups of women in the iLiNS (International Lipid-based Nutrient Supplements)-DYAD trial in Ghana.Methods: Women (n = 1320; <20 wk of gestation) were randomly assigned to receive 60 mg Fe + 400 μg folic acid/d (IFA), 18 vitamins and minerals/d [multiple micronutrients (MMNs)], or 20 g SQ-LNSs with 22 micronutrients/d (LNS) during pregnancy and a placebo (200 mg Ca/d), MMNs, or SQ-LNSs, respectively, for 6 mo postpartum...
March 8, 2017: Journal of Nutrition
https://www.readbyqxmd.com/read/28270223/biomechanical-characteristics-of-fixation-methods-for-floating-pubic-symphysis
#9
Wenhao Song, Dongsheng Zhou, Yu He
BACKGROUND: Floating pubic symphysis (FPS) is a relatively rare injury caused by high-energy mechanisms. There are several fixation methods used to treat FPS, including external fixation, subcutaneous fixation, internal fixation, and percutaneous cannulated screw fixation. To choose the appropriate fixation, it is necessary to study the biomechanical performance of these different methods. The goal of this study was to compare the biomechanical characteristics of six methods by finite element analysis...
March 7, 2017: Journal of Orthopaedic Surgery and Research
https://www.readbyqxmd.com/read/28268195/-candidatus-rickettsia-nicoyana-a-novel-rickettsia-species-isolated-from-ornithodoros-knoxjonesi-in-costa-rica
#10
Rolando D Moreira-Soto, Andrés Moreira-Soto, Eugenia Corrales-Aguilar, Ólger Calderón-Arguedas, Adriana Troyo
Rickettsiae are intracellular bacteria commonly associated with hematophagous arthropods. Most of them have been described in hard ticks, but some have been found in soft ticks. Here we report the detection and isolation of a new Rickettsia from Ornithodoros knoxjonesi larvae collected from Balantiopteryx plicata (Emballonuridae) in Nicoya, Costa Rica. Two ticks were processed to detect Rickettsia spp. genes gltA, ompA, ompB, and htrA by PCR. Part of the macerate was also inoculated into Vero E6 and C6/36 cell lines, and cells were evaluated by Giménez stain, indirect immunofluorescence assay (IFA), and PCR...
February 27, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28250195/adherence-specific-social-support-enhances-adherence-to-calcium-supplementation-regimens-among-pregnant-women
#11
Stephanie L Martin, Moshood O Omotayo, Gretel H Pelto, Gina M Chapleau, Rebecca J Stoltzfus, Katherine L Dickin
Background: WHO guidelines recommend integrating calcium supplementation into antenatal care (ANC) alongside iron and folic acid (IFA) to reduce maternal mortality. However, supplementation programs face multiple barriers, and strategies to improve adherence are needed. An adherence partner is someone whom pregnant women ask to support adherence at home.Objectives: This study 1) assessed adherence partner acceptability, feasibility, and associations with calcium and IFA supplement adherence and 2) examined relations between social support and adherence...
March 1, 2017: Journal of Nutrition
https://www.readbyqxmd.com/read/28249707/bartonella-species-pathogenic-for-humans-infect-pets-free-ranging-wild-mammals-and-their-ectoparasites-in-the-caatinga-biome-northeastern-brazil-a-serological-and-molecular-study
#12
Mariana Campos Fontalvo, Alexsandra Rodrigues de Mendonça Favacho, Andreina de Carvalho Araujo, Naylla Mayana Dos Santos, Glauber Meneses Barboza de Oliveira, Daniel Moura Aguiar, Elba Regina Sampaio de Lemos, Mauricio Claudio Horta
This study verified the occurrence of Bartonella spp. in dogs, cats, wild mammals and their ectoparasites in Petrolina and Lagoa Grande Counties, Pernambuco, located in a semi-arid region in Northeastern Brazil. Anti-Bartonella spp. antibodies were detected by indirect immunofluorescence assay (IFA) in 24.8% of dogs (27/109) and in 15% of cats (6/40). Bartonella sp. DNA was identified by PCR performed on DNA extracted from blood and ectoparasites using primers targeting Bartonella sp. gltA and ribC genes in 100% (9/9) of Pulex irritans from Cerdocyon thous, 57...
February 26, 2017: Brazilian Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28243851/inactivation-of-kupffer-cells-by-selenizing-astragalus-polysaccharides-prevents-ccl4-induced-hepatocellular-necrosis-in-the-male-wistar-rat
#13
Mohammed Hamid, Dandan Liu, Yassin Abdulrahim, Alamzeb Khan, Gang Qian, Kehe Huang
Selenizing astragalus polysaccharides-3 (sAPS3) was prepared by nitric acid-sodium selenite method. The effects of sAPS3 on carbon tetrachloride (CCl4) induced hepatocellular necrosis, and its underlying mechanisms were studied in male Wistar rats. Hepatic damage was induced by intraperitoneal injection of CCl4 twice a week, for 3 weeks. Meanwhile, the rats in addition to CCl4 were also exposed to sodium selenite (SS), astragalus polysaccharides (APS), SS + APS or sAPS3, in parallel by oral gavage once a day for 3 weeks...
February 27, 2017: Biological Trace Element Research
https://www.readbyqxmd.com/read/28237695/seroprevalence-study-of-toscana-virus-and-viruses-belonging-to-the-sandfly-fever-naples-antigenic-complex-in-central-and-southern-italy
#14
Serena Marchi, Claudia M Trombetta, Otfried Kistner, Emanuele Montomoli
Sandfly fever viruses are transmitted by the bite of phlebotomine sandflies; serotypes sandfly fever Naples virus, sandfly fever Sicilian virus and sandfly fever Cyprus virus cause febrile illness, whereas Toscana virus (TOSV) may cause neuroinvasive infections. Although TOSV is an important cause of aseptic meningitis in central and southern Italy, in many cases the infection is asymptomatic, leading to underestimation of the actual spread of the virus. This serosurvey aimed to assess the seroprevalence of TOSV in a random population in Siena (Tuscany, central Italy) in 2003-2004 and 2013-2014 and Bari (Apulia, southern Italy) in 2004 and 2015...
February 22, 2017: Journal of Infection and Public Health
https://www.readbyqxmd.com/read/28235730/effect-of-organic-loading-rates-on-biodegradation-of-linear-alkyl-benzene-sulfonate-oil-and-grease-in-greywater-by-integrated-fixed-film-activated-sludge-ifas
#15
Hadi Eslami, Mohammad Hassan Ehrampoush, Mohammad Taghi Ghaneian, Mehdi Mokhtari, Aliasghar Ebrahimi
In this study, performance of Integrated Fixed-film Activated Sludge (IFAS) system in treatment of Linear Alkylbenzene Sulfonate (LAS), and oil & grease in synthetic greywater and effect of Organic Loading Rates (OLRs) on removal efficiency within a period of 105 days were investigated. Present study was carried out in a pilot scale under such conditions as temperature of 30 ± 1 °C, dissolved oxygen of 2.32 ± 0.91 mg/l, pH of 8.01 ± 0.95 and OLRs of 0.11-1.3gCOD/L.d. Also, Scanning Electron Microscopy (SEM) images were employed to specify rate of the biofilm formed on the media inside the reactor IFAS...
May 15, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28223026/development-and-application-of-nsp5-elisa-for-the-detection-of-antibody-to-infectious-bronchitis-virus
#16
Jing Lei, Tingting Shi, Dongnan Sun, Kaikun Mo, Yan Yan, Yulan Jin, Min Liao, Jiyong Zhou
Infectious bronchitis virus (IBV) continues to be one of the most important poultry pathogens worldwide. The current commercially available enzyme-linked immunosorbent assay (ELISA) kits for IBV specific antibody detection are mostly based on the whole virion, and few serological tests based on nonstructural proteins of IBV have been developed. Herein, an alternative indirect ELISA for detection of IBV antibody was developed with IBV nonstructural protein 5 (nsp5) produced by Escherichia coli. Using an indirect immunofluorescence assay (IFA) and a commercial ELISA kit as reference, we optimized the nsp5-ELISA and determined its cut-off as 0...
May 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28219125/issue-information-ifa
#17
(no author information available yet)
No abstract text is available yet for this article.
February 2017: Journal of Hepato-biliary-pancreatic Sciences
https://www.readbyqxmd.com/read/28207460/anthropometric-outcome-measures-in-patients-with-metopic-craniosynostosis
#18
Scott J Farber, Dennis C Nguyen, Gary B Skolnick, Sybill D Naidoo, Matthew D Smyth, Kamlesh B Patel
PURPOSE: Treatment of metopic craniosynostosis is performed by either fronto-orbital advancement (FOA) or endoscopic-assisted techniques. Interfrontal angle (IFA) is a validated measure of trigonocephaly, but requires a computed tomography scan. The most common direct measure to assess surgical outcome in patients with trigonocephaly is frontal width (ft-ft). The aim of this study is to determine if frontal width correlates with IFA and successful surgical correction 1 year after treatment...
February 15, 2017: Journal of Craniofacial Surgery
https://www.readbyqxmd.com/read/28202789/use-of-multiplex-real-time-pcr-to-diagnose-scrub-typhus
#19
Wiwit Tantibhedhyangkul, Ekkarat Wongsawat, Saowaluk Silpasakorn, Duangdao Waywa, Nuttawut Saenyasiri, Jintapa Suesuay, Wilawan Thipmontree, Yupin Suputtamongkol
Scrub typhus, caused by Orientia tsutsugamushi, is a common cause of acute undifferentiated febrile illness in the Asia--Pacific region. However, its non-specific clinical manifestation often prevents early diagnosis. We propose the use of PCR and serologic tests as diagnostic tools. Here, we developed a multiplex real-time PCR assay using hydrolysis (Taqman) probes targeting O. tsutsugamushi47-kDa, groEL, and human IFNB genes to improve early diagnosis of scrub typhus. The amplification efficiency was higher than 94%, and the lower detection limit was 10 copies per reaction...
February 15, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28202026/an-indirect-enzyme-linked-immunosorbent-assay-for-the-identification-of-antibodies-to-senecavirus-a-in-swine
#20
Cheryl M T Dvorak, Zeynep Akkutay-Yoldar, Suzanne R Stone, Steven J P Tousignant, Fabio A Vannucci, Michael P Murtaugh
BACKGROUND: Senecavirus A (SVA), a member of the family Picornaviridae, genus Senecavirus, is a recently identified single-stranded RNA virus closely related to members of the Cardiovirus genus. SVA was originally identified as a cell culture contaminant and was not associated with disease until 2007 when it was first observed in pigs with Idiopathic Vesicular Disease (IVD). Vesicular disease is sporadically observed in swine, is not debilitating, but is significant due to its resemblance to foreign animal diseases, such as foot-and-mouth disease (FMD), whose presence would be economically devastating to the United States...
February 15, 2017: BMC Veterinary Research
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