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https://www.readbyqxmd.com/read/28929965/-a-negleted-bacteria-with-a-case-bartonella-henselae
#1
Kamuran Türker, Bekir Çelebi, Şeyda Andaç, Pınar Bulut, Şenay Yalçın, Saniye Dolhan
Bartonella henselae the causative agent of cat scratch disease (CSD), is a gram-negative, coccobacillus, facultative intracellular bacterium CSD usually presents as a clinical form of benign local lymphadenopathy (LAP) but sometimes it may progress to severe life threatening complications. Despite the fact that CSD is known to be a common disease, which is one of the important causes of local LAPs in the world, there are few publications in our country. For the diagnosis, the clinician should suspect for CSD and has to ask to the patient whether there is a story of cat scratch or not...
July 2017: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/28924978/using-formative-research-to-promote-antenatal-care-attendance-and-iron-folic-acid-supplementation-in-zinder-niger
#2
Sonja Y Hess, Césaire T Ouédraogo, Ibrahim F Bamba, K Ryan Wessells, Nancy Keith, Thierno Faye, Banda Ndiaye, Maimouna Doudou, Jennifer Nielsen
In Niger, use of antenatal care (ANC) and iron folic acid (IFA) supplements is suboptimal. The objectives of this paper are as follows: (a) to conduct formative research to understand barriers and beliefs among pregnant women related to ANC, IFA supplementation, and pregnancy outcomes; (b) assess the quality of currently provided ANC services; (c) use the findings to guide the development of programmatic interventions to improve coverage of ANC services and IFA supplementation of pregnant women. Structured in-home interviews (n = 72) and focus groups (n = 4) were conducted with pregnant women in 4 randomly selected villages in rural Zinder...
September 19, 2017: Maternal & Child Nutrition
https://www.readbyqxmd.com/read/28922404/eco-epidemiological-analysis-of-rickettsial-seropositivity-in-rural-areas-of-colombia-a-multilevel-approach
#3
Juan C Quintero V, Luis E Paternina T, Alexander Uribe Y, Carlos Muskus, Marylin Hidalgo, Juliana Gil, Astrid Vanessa Cienfuegos G, Lisardo Osorio Q, Carlos Rojas A
Rickettsiosis is a re-emergent infectious disease without epidemiological surveillance in Colombia. This disease is generally undiagnosed and several deadly outbreaks have been reported in the country in the last decade. The aim of this study is to analyze the eco-epidemiological aspects of rickettsial seropositivity in rural areas of Colombia where outbreaks of the disease were previously reported. A cross-sectional study, which included 597 people living in 246 households from nine hamlets in two municipalities of Colombia, was conducted from November 2015 to January 2016...
September 18, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28917325/comparison-of-five-diagnostic-tests-for-giardia-duodenalis-in-fecal-samples-from-young-dogs
#4
Fabienne D Uehlinger, S Ali Naqvi, Spencer J Greenwood, J Trenton McClure, Gary Conboy, Ryan O'Handley, Herman W Barkema
Five diagnostic tests were compared for the diagnosis of Giardia duodenalis in fecal samples of young dogs. Fecal samples were collected from 136 healthy dogs <1year old and examined using immunofluorescence antibody microscopy (IFA) after sucrose gradient centrifugation, zinc sulfate centrifugal flotation technique (ZSCT), SNAP(®)Giardia test, and ProSpecT(®)Giardia EZ Microplate assay. In addition, polymerase chain reaction (PCR) of the 16S rRNA gene was performed. Kappa (κ) statistic was calculated to assess diagnostic agreement between the IFA and each test...
September 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28915999/oxidative-stress-in-dairy-cows-seropositives-for-neospora-caninum
#5
Patrícia Glombowsky, Nathieli B Bottari, Vanderlei Klauck, Juscivete F Fávero, Natan M Soldá, Matheus D Baldissera, Gessica Perin, Vera M Morsch, Maria Rosa C Schetinger, Lenita M Stefani, Aleksandro S Da Silva
Bovine neosporosis is caused by the protozoan Neospora caninum and is one of the major causes of abortion in cows. Cattle are intermediate hosts of this parasite and may have asymptomatic or symptomatic infections. Therefore, the aim of this study was to evaluate oxidative stress marker reactive oxygen species (ROS), thiobarbituric reactive acid substances (TBARS) levels, glutathione S-transferase (GST), adenosine deaminase (ADA), and butyrylcholinesterase (BChE) activities in dairy cows seropositives for N...
October 2017: Comparative Immunology, Microbiology and Infectious Diseases
https://www.readbyqxmd.com/read/28911844/serologic-responses-to-peptides-of-anaplasma-phagocytophilum-and-borrelia-burgdorferi-in-dogs-infested-with-wild-caught-ixodes-scapularis
#6
R Chandrashekar, M J Beall, B Thatcher, J M Saucier, P Tyrrell, M R Lappin
Anaplasma phagocytophilum and Borrelia burgdorferi are both transmitted by Ixodes spp. and are associated with clinical illness in some infected dogs. This study evaluated canine antibody responses to the A. phagocytophilum p44 peptides APH-1 and APH-4 as well as the B. burgdorferi C6 peptide before and after doxycycline treatment. A total of eight dogs were infested with wild-caught I. scapularis for 1 week. Blood was collected prior to tick attachment and from Days 3-77 to 218-302 with doxycycline treatment beginning on Day 218...
August 2017: Veterinary Journal
https://www.readbyqxmd.com/read/28888478/mandibular-coronoidectomy-could-significantly-accelerate-the-healing-process-of-infratemporal-fossa-abscess
#7
Yi-Wen Liu, Krishna Prasad Regmi, Ming-Chao Ding, Yan Li, Lei Tian, Bo-Lei Cai
PURPOSE: Management of an infratemporal fossa abscess (IFA), which is a specific form of severe and advanced deep fascial space infection (DFI), is based mainly on traditional methods. The purpose of this study was to investigate the role of mandibular coronoidectomy in accelerating IFA healing. PATIENTS AND METHODS: This research is a single-center retrospective study composed of 23 patients with IFA. The predictor variables were gender, age, diabetes, severity score, and mandibular coronoidectomy...
August 17, 2017: Journal of Oral and Maxillofacial Surgery
https://www.readbyqxmd.com/read/28887100/verification-of-post-chemotherapeutic-clearance-of-theileria-equi-through-concordance-of-nested-pcr-and-immunoblot
#8
L N Wise, L S Kappmeyer, M G Silva, S N White, J F Grause, D P Knowles
Certain countries including the United States remain non-endemic for particular infectious diseases such as equine piroplasmosis through import restrictions and surveillance. Endemic regions often employ premunition as the primary method to control disease, however in non-endemic countries, chemosterilization combined with methods to confirm parasite elimination are required to maintain disease-free status. The ability of imidocarb diproprionate (ID) to clear persistent Theileria equi infection from infected horses has been shown through the inability of treated horses to transmit via blood transfer...
August 25, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28885292/incidence-and-risk-factors-of-anterior-arch-fracture-of-the-atlas-following-c1-laminectomy-without-fusion
#9
Takayoshi Shimizu, Bungo Otsuki, Shunsuke Fujibayashi, Shinji Kumamoto, Yasukazu Hijikata, Yu Shimizu, Mitsuru Takemoto, Masato Ota, Hideo Ito, Shuichi Matsuda
STUDY DESIGN: A retrospective multi-center study OBJECTIVE.: To identify the incidence of anterior arch fracture (AAF) of the atlas following C1 (first cervical vertebra) laminectomy without fusion, and its risk factors. SUMMARY OF BACKGROUND DATA: C1 laminectomy without fusion is a widely accepted surgical procedure performed to decompress the spinal cord that is compromised at the C1/C2 level, but without instability. Several case series have reported spontaneous AAF following this procedure...
September 6, 2017: Spine
https://www.readbyqxmd.com/read/28882612/proposed-panel-of-diagnostic-tools-for-accurate-temporal-classification-of-symptomatic-t-gondii-infection
#10
Geisa Baptista Barros, Elenice Moreira Lemos, Priscila Pinto E Silva-Dos-Santos, Reynaldo Dietze, Eliana Zandonade, José Roberto Mineo, Deise Aparecida de Oliveira Silva, Ana Cláudia Marquez Pajuaba, Matheus de Souza Gomes, Laurence Rodrigues do Amaral, Jordana Grazziela Coelho-Dos-Reis, Olindo Assis Martins-Filho, José Carlos Serufo
Serological tests available for the diagnosis of acute Toxoplasma gondii infection have limitations in establishing the temporal diagnosis of acute toxoplasmosis. The present analytical-descriptive investigation comprises of a prospective longitudinal cohort study to search for accurate biomarkers to distinguish acute, early and late convalescent T. gondii infection. Classic methods (immunofluorescence-IFA along with Enzyme-linked immunosorbent-ELISA and fluorescent-ELFA assays) for IgM, IgA, IgG and IgG avidity were employed in parallel with flow cytometry-based anti-fixed T...
September 4, 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/28878522/validation-of-geno-sen-s-scrub-typhus-real-time-polymerase-chain-reaction-kit-by-its-comparison-with-a-serological-elisa-test
#11
Velmurugan Anitharaj, Selvaraj Stephen, Jothimani Pradeep, Pratheesh Pooja, Sridharan Preethi
BACKGROUND: In the recent past, scrub typhus (ST) has been reported from different parts of India, based on Weil-Felix/enzyme-linked immunosorbent assay (ELISA)/indirect immunofluorescence assay (IFA). Molecular tests are applied only by a few researchers. AIMS: Evaluation of a new commercial real time polymerase chain reaction (PCR) kit for molecular diagnosis of ST by comparing it with the commonly used IgM ELISA is our aim. SETTINGS AND DESIGN: ST has been reported all over India including Puducherry and surrounding Tamil Nadu and identified as endemic for ST...
July 2017: Journal of Global Infectious Diseases
https://www.readbyqxmd.com/read/28878035/a-simplified-regimen-compared-with-who-guidelines-decreases-antenatal-calcium-supplement-intake-for-prevention-of-preeclampsia-in-a-cluster-randomized-noninferiority-trial-in-rural-kenya
#12
Moshood O Omotayo, Katherine L Dickin, David L Pelletier, Erick O Mwanga, Jacqueline K Kung'u, Rebecca J Stoltzfus
Background: To prevent preeclampsia, the WHO recommends antenatal calcium supplementation in populations with inadequate habitual intake. The WHO recommends 1500-2000 mg Ca/d with iron-folic acid (IFA) taken separately, a complex pill-taking regimen. Objective: The objective of this study was to test the hypothesis that simpler regimens with lower daily dosages would lead to higher adherence and similar supplement intake.Methods: In the Micronutrient Initiative Calcium Supplementation study, we compared the mean daily supplement intake associated with 2 dosing regimens with the use of a parallel, cluster-randomized noninferiority trial implemented in 16 primary health care facilities in rural Kenya...
September 6, 2017: Journal of Nutrition
https://www.readbyqxmd.com/read/28865205/investigation-of-the-bone-metabolism-in-dogs-with-leishmaniasis
#13
M Ari, A Bildik, S Ertug
The present study was aimed at determining bone metabolism related changes in dogs with visseral leishmaniasis such as vitamin D, calcium, phosphate and ALP levels as compared to healthy ones. A total of 40 serums of male dogs aged between 4 and 6 years that were sent to Adnan Menderes University, Faculty of Medicine, Parasitology Laboratory from Kuşadasy and Bodrum districts between the years 2012 and 2013, suspected with leishmaniasis, by the veterinary physicians in accordance with the cold chain rules and 20 of which were evaluated as leishmania seropositive and 20 as leishmania seronegative by the IFA test, were included in this study...
March 1, 2017: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/28862278/correction-rapid-determination-of-the-tumour-stroma-ratio-in-squamous-cell-carcinomas-with-desorption-electrospray-ionization-mass-spectrometry-desi-ms-a-proof-of-concept-demonstration
#14
Michael Woolman, Alessandra Tata, Delaram Dara, Jalna Meens, Elisa D'Arcangelo, Consuelo J Perez, Shamina Saiyara Prova, Emma Bluemke, Howard J Ginsberg, Demian Ifa, Alison McGuigan, Laurie Ailles, Arash Zarrine-Afsar
Correction for 'Rapid determination of the tumour stroma ratio in squamous cell carcinomas with desorption electrospray ionization mass spectrometry (DESI-MS): a proof-of-concept demonstration' by Michael Woolman et al., Analyst, 2017, 142, 3250-3260.
September 1, 2017: Analyst
https://www.readbyqxmd.com/read/28841878/the-role-of-lutheran-basal-cell-adhesion-molecule-in-human-bladder-carcinogenesis
#15
Hong-Yi Chang, Hsin-Mei Chang, Tsung-Jung Wu, Chang-Yao Chaing, Tzong-Shin Tzai, Hong-Lin Cheng, Giri Raghavaraju, Nan-Haw Chow, Hsiao-Sheng Liu
BACKGROUND: Lutheran/basal cell adhesion molecule (Lu/BCAM) is a membrane bound glycoprotein. This study was performed to investigate the role and downstream signaling pathway of Lu/BCAM in human bladder tumorigenesis. METHODS: Five human bladder cancer (E6, RT4, TSGH8301, TCCSUP and J82), one stable mouse fibroblast cell line (NIH-Lu) expressing Lu/BCAM transgene and sixty human uroepithelial carcinoma specimens were analyzed by real-time PCR, immunohistochemistry (IHC), immunofluorescence (IFA) staining, Western blotting and promoter luciferase assay for Lu/BCAM, respectively...
August 26, 2017: Journal of Biomedical Science
https://www.readbyqxmd.com/read/28826782/micrognathia-and-oropharyngeal-space-in-patients-with-robin-sequence-prenatal-mri-measurements
#16
Tessa D Kooiman, Carly E Calabrese, Ryne Didier, Judy A Estroff, Bonnie L Padwa, Maarten J Koudstaal, Cory M Resnick
PURPOSE: Micrognathia is the initiating feature of Robin sequence (RS) and leads to airway obstruction. Prenatal identification of micrognathia is currently qualitative and has not correlated with postnatal findings in previous studies. Oropharyngeal airway space has not been evaluated prenatally. The purposes of this study were to 1) quantitate mandibular characteristics and oropharyngeal size at prenatal magnetic resonance imaging (MRI) and 2) identify differences in fetuses with postnatal RS compared with those with micrognathia (without RS) and normal controls...
July 26, 2017: Journal of Oral and Maxillofacial Surgery
https://www.readbyqxmd.com/read/28818711/the-influence-of-solid-retention-time-on-ifas-mbr-systems-assessment-of-nitrous-oxide-emission
#17
Giorgio Mannina, Marco Capodici, Alida Cosenza, Vito Armando Laudicina, Daniele Di Trapani
The aim of the present study was to investigate the nitrous oxide (N2O) emissions from a moving bed based Integrated Fixed Film Activated Sludge (IFAS) - membrane bioreactor (MBR) pilot plant, designed according to the University of Cape Town (UCT) layout. The experimental campaign had a duration of 110 days and was characterized by three different sludge retention time (SRT) values (∞, 30 d and 15 d). Results highlighted that N2O concentrations decreased when the biofilm concentrations increased within the aerobic reactor...
August 14, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28815296/autoantibodies-against-rods-and-rings-related-impdh2-in-hepatitis-c-genotype-1-and-daa-therapy-in-a-real-life-cohort
#18
Werner Dammermann, Susanne Polywka, Inga Dettmann, Swantje Mindorf, Lars Komorowski, Malte Wehmeyer, Julian Schulze Zur Wiesch, Winfried Stöcker, Stefan Lüth
Autoantibodies against inosine-5'-monophosphate-dehydrogenase-2 (IMPDH2; "rods and rings" pattern) develop in chronic hepatitis C (CHC) patients under treatment with peg-interferon (IFN) and ribavirin (RBV), an inhibitor of IMPDH2. We investigated the influence of the alternative therapy with direct-acting antivirals (DAA)/ribavirin on anti-IMPDH2 autoantibody generation and the use of anti-IMPDH2 development as a marker for therapy outcome (sustained virologic response, SVR). We analyzed a "real life" cohort of 104 unselected CHC genotype 1 (GT1) patients treated with IFN/first-generation DAA/RBV prospectively compared to a historic cohort of 59 IFN/RBV-treated CHC GT1 patients...
August 16, 2017: Medical Microbiology and Immunology
https://www.readbyqxmd.com/read/28799592/rapid-determination-of-the-tumour-stroma-ratio-in-squamous-cell-carcinomas-with-desorption-electrospray-ionization-mass-spectrometry-desi-ms-a-proof-of-concept-demonstration
#19
Michael Woolman, Alessandra Tata, Delaram Dara, Jalna Meens, Elisa D'Arcangelo, Consuelo J Perez, Shamina Saiyara Prova, Emma Bluemke, Howard J Ginsberg, Demian Ifa, Alison McGuigan, Laurie Ailles, Arash Zarrine-Afsar
Squamous cell carcinomas constitute a major class of head & neck cancers, where the tumour stroma ratio (TSR) carries prognostic information. Patients affected by stroma-rich tumours exhibit a poor prognosis and a higher chance of relapse. As such, there is a need for a technology platform that allows rapid determination of the tumour stroma ratio. In this work, we provide a proof-of-principle demonstration that Desorption Electrospray Ionization Mass Spectrometry (DESI-MS) can be used to determine tumour stroma ratios...
August 11, 2017: Analyst
https://www.readbyqxmd.com/read/28795800/prompt-and-robust-humoral-immunity-elicited-by-a-conjugated-chimeric-malaria-antigen-with-a-truncated-flagellin
#20
Fangxia Guo, Yongdong Liu, Chun Zhang, Qi Wang, Lianyan Wang, Yuhui Gao, Jingxiu Bi, Heng Wang, Zhiguo Su
As one of the pathogen-associated molecular patterns (PAMPs), flagellin is recently utilized as a potent adjuvant for many subunit vaccines. In this study, a truncated flagellin (tFL) with deletion of the hypervariable regions was adopted as a carrier-adjuvant by chemical conjugation with a chimeric malaria antigen M.RCAg-1 (M312) via a heterobifunctional polyethylene glycol (PEG) linker. After booster immunization in mice without any extra adjuvants, the M312-PEG-tFL conjugates elicited M312-specific antibody titers 100-1000 times higher than M312 and 10-100 times higher than the physical mixture of M312 and tFL...
August 22, 2017: Bioconjugate Chemistry
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