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Robabeh Abedini, Maryam Nasimi, Pedram Nourmohammad Pour, Ifa Etesami, Safa Al-Asiri, Hamid Reza Tohidinik
There is little known about illness perception in patients with skin tumors. We conducted this study to investigate Iranian patients' understanding of skin tumors, and to evaluate their sun-protective behavior changes after treatment of skin cancer. Patients with a skin biopsy of basal cell carcinoma were asked to complete questionnaires. A total of 110 patients were enrolled in the study. Patients were mostly referred to our tumor clinic from rural areas. At the skin cancer perception investigation, 63% of patients did not consider their disease as a long-lasting situation...
November 15, 2017: Journal of Cancer Education: the Official Journal of the American Association for Cancer Education
Yunxiao Sun, Zhen Peng, Libiao Zhang, Huaqian Wang, Xiangyang He, Xingwen Peng, Qin Zhang, Hui Liu, Junhua Rao, Haifeng Wang, Jie Wu
Experimental autoimmune encephalomyelitis (EAE) induced by self-myelin antigen is a widely used multiple sclerosis (MS) model for preclinical studies of new therapeutics and potential pathogenesis. By comparison with rodent EAE models, EAE models in primates are more similar to MS. Some groups have developed EAE models in primates by using common marmoset (Callithrix jacchus). However, this model has some limitations. EAE in cynomolgus monkey (Macaque fasciculrais) could overcome these limitations. In this study, EAE was induced in cynomolgus monkey by immunizing with the recombinant human myelin oligodendrocyte glycoprotein extracellular domain (1-125) (rhMOG1-125) and a synthetic peptide, representing peptide 34-56 of human myelin oligodendrocyte glycoprotein (MOG34-56) in complete Freund's adjuvant (CFA) and in combination with intravenous injection of pertussis toxin, and subsequent booster immunizations with the same dose of antigen in incomplete Freund's adjuvant (IFA) until the animals developed clinically significant EAE (score≥2)...
November 9, 2017: Protein and Peptide Letters
Jian Wang, Wenqi Zheng, Fei Liu, Yaru Wang, Yiwen He, Li Zheng, Qi Fan, Enjie Luo, Yaming Cao, Liwang Cui
BACKGROUND: A vaccine that targets multiple developmental stages of malaria parasites would be an effective tool for malaria control and elimination. METHODS: A conserved gene in Plasmodium, the Plasmodium berghei gene (PBANKA_020570) encoding a 51 kDa protein (pb51 gene), was identified through search of the PlasmoDB database using a combination of expression and protein localization criteria. A partial domain of the Pb51 protein was expressed in a prokaryotic expression system (rPb51) and used for immunization in mice...
November 13, 2017: Malaria Journal
Patrick John Kelly, Liza Köster, Jing Li, Jilei Zhang, Ke Huang, Gillian Carmichael Branford, Silvia Marchi, Michel Vandenplas, Chengming Wang
BACKGROUND: As there is little data on vector-borne diseases of cats in the Caribbean region and even around the world, we tested feral cats from St Kitts by PCR to detect infections with Babesia, Ehrlichia and spotted fever group Rickettsia (SFGR) and surveyed them for antibodies to Rickettsia rickettsii and Ehrlichia canis. RESULTS: Whole blood was collected from apparently healthy feral cats during spay/ neuter campaigns on St Kitts in 2011 (N = 68) and 2014 (N = 52)...
November 13, 2017: BMC Veterinary Research
Paul Moretti, Jean-Marc Choubert, Jean-Pierre Canler, Pierre Buffière, Olivier Pétrimaux, Paul Lessard
The integrated fixed-film activated sludge (IFAS) process is being increasingly used to enhance nitrogen removal for former activated sludge systems. The aim of this work is to evaluate a numerical model of a new nitrifying/denitrifying IFAS configuration. It consists of two carrier-free reactors (anoxic and aerobic) and one IFAS reactor with a filling ratio of 43% of carriers, followed by a clarifier. Simulations were carried out with GPS-X involving the nitrification reaction combined with a 1D heterogeneous biofilm model, including attachment/detachment processes...
November 10, 2017: Bioprocess and Biosystems Engineering
Andrew E Yang, Charles A Hartranft, Alisha Reiss, Charles R Holden
OBJECTIVES: Nearly 25% of vascular surgery patients operated on for peripheral vascular disease are readmitted within 30 days. Development of adjuncts to minimize wound complications remains an area of great interest. Here, we present our experience with intraoperative fluorescent angiography (IFA) as a tool to predict skin flap viability following lower extremity amputations (LEAs). METHODS: Experience with IFA using indocyanine green (ICG) dye was reviewed retrospectively at a single institution...
January 1, 2017: Vascular and Endovascular Surgery
Hadjila Yanes, Aurélie Hennebique, Isabelle Pelloux, Sandrine Boisset, Dominique J Bicout, Yvan Caspar, Max Maurin
Tularemia is a zoonosis caused by the bacterium Francisella tularensis Its specific diagnosis remains based on serological methods, while detection of F. tularensis in clinical samples by culture or PCR is rarely obtained. The aim of the present study was to evaluate the performance of the SERION ELISA classic Francisella tularensis IgG and IgM tests (Virion/Serion GmbH Institute, Würzburg, Germany) and the VIRapid® Tularemia immunochromatographic (ICT) test (VIRCELL, Granada, Spain), compared to the in-house microagglutination (MAT) and indirect immunofluorescence (IFA) tests currently used at the French National Reference Centre for Francisella...
November 8, 2017: Journal of Clinical Microbiology
Joice Faria, Caroline Couto, Sheron Wierzynski, Nathieli Bottari, Matheus Baldissera, Wanderson Pereira, Aleksandro Da Silva
Toxoplasma gondii is a cosmopolitan protozoan that causes disease in several species, including humans. In cats, these infections are usually asymptomatic, but in other species it can lead to high levels of inflammatory and cell damage markers, which may cause cellular damage. Therefore, the aim of this study was to measure levels of tumor necrosis factor (TNF-α), reactive oxygen species (ROS), and nitric oxide (nitrite/nitrate - NOx) in the serum of cats seropositive for T. gondii. Initially, we investigated the presence of antibodies against T...
November 10, 2017: Journal of Parasitology
José A Oteo, Ricardo Maggi, Aránzazu Portillo, Julie Bradley, Lara García-Álvarez, Montserrat San-Martín, Xavier Roura, Edward Breitschwerdt
BACKGROUND: The genus Bartonella includes fastidious, facultative intracellular bacteria mainly transmitted by arthropods and distributed among mammalian reservoirs. Bartonella spp. implicated as etiological agents of zoonoses are increasing. Apart from the classical Bartonella henselae, B. bacilliformis or B. quintana, other species (B. elizabethae, B. rochalimae, B. vinsonii arupensis and B. v. berkhoffii, B. tamiae or B. koehlerae, among others) have also been associated with human and/or animal diseases...
November 7, 2017: Parasites & Vectors
Ganesh Tadepalli, Balakrishna Konduru, Harishchandra Sripathy Murali, Harsh Vardhan Batra
Recombinant engineering of immunologically active chimeric protein consisting of Omp19 and P39 domains of B. abortus (rOP), was purified under denaturing conditions upon expression in E. coli BL21 (DE3) and refolded to dynamic form. The immuno-protective efficacy of rOP was evaluated by challenging the BALB/c mice intraperitoneally (I.P) with the infective species of Brucella in the absence or presence of adjuvants, such as Aluminum hydroxide gel (Al), or Freund's Complete Adjuvant (FCA)/Incomplete Freund's Adjuvant (IFA)...
October 26, 2017: Immunology Letters
Junhao Chen, Ruihua Zhang, Shaoli Lin, Pengfei Li, Jingjing Lan, Zhijing Xie, Yu Wang, Shijin Jiang
BACKGROUND: DNA-launched infectious system is a useful tool with high rescue efficiency that allows the introduction of mutations in specific positions to investigate the function of an individual viral element. Rescued virus particles could be harvested by directly transfecting the DNA-launched recombinant plasmid to the host cells, which will reduce labor and experimental cost by skipping the in vitro transcription assay. METHODS: A total of four overlapping fragments covering the entire viral genome were amplified and then were assembled into a transformation vector based on pIRES2-EGFP to establish the DNA-launched infectious system of duck hepatitis A virus type 1 (DHAV-1), named pIR-DHAV-1...
November 3, 2017: Virology Journal
Yuhao Sun, Xiaolin Chen, Ziqiang Cheng, Song Liu, Huahua Yu, Xueqin Wang, Pengcheng Li
In this study, polysaccharides from Grateloupia filicinia (GFP) were extracted and several low molecular weight (Mw) G. filicina polysaccharides (LGFPs) were prepared by the hydrogen peroxide (H₂O₂) oxidation method. Additionally, the effect of different experimental conditions on the degradation of GFP was determined. Results showed that the GFP degradation rate was positively related to H₂O₂ concentration and temperature, and negatively related to pH. Chemical analysis and Fourier transform infrared spectra (FT-IR) of GFP and LGFPs showed that the degradation caused a slight decrease of total sugar and sulfate content...
November 3, 2017: Marine Drugs
Elizabeth L Prado, Ulla Ashorn, John Phuka, Kenneth Maleta, John Sadalaki, Brietta M Oaks, Marjorie Haskell, Lindsay H Allen, Steve A Vosti, Per Ashorn, Kathryn G Dewey
Pregnant and post-partum women require increased nutrient intake and optimal cognition, which depends on adequate nutrition, to enable reasoning and learning for caregiving. We aimed to assess (a) differences in maternal cognition and caregiving between women in Malawi who received different nutritional supplements, (b) 14 effect modifiers, and (c) associations of cognition and caregiving with biomarkers of iron, Vitamin A, B-vitamin, and fatty acid status. In a randomized controlled trial (n = 869), pregnant women daily received either multiple micronutrients (MMN), 20 g/day lipid-based nutrient supplements (LNS), or a control iron/folic acid (IFA) tablet...
November 2, 2017: Maternal & Child Nutrition
(no author information available yet)
No abstract text is available yet for this article.
November 2017: Journal of Hepato-biliary-pancreatic Sciences
Ken-Ichiro Otsuyama, Hidehiro Tsuneoka, Hiroka Yoshidomi, Mio Haraguchi, Masashi Yanagihara, Nobuko Tokuda, Junzo Nojima, Kiyoshi Ichihara
We evaluated the utility of Western blot (WB) bands of Bartonella henselae in detecting anti-B. henselae immunoglobulin M (IgM) for serodiagnosis of cat scratch disease (CSD). IgM band patterns were examined using sera from 92 patients clinically suspected of having CSD and from 130 healthy individuals. Positive WB bands were observed in 49 (53.5%) of the 92 patients' sera. Three bands at 8--10, 31--35, and 70 kDa were regarded as relevant for B. henselae because all of the positive sera yielded at least one of the three bands, and none of the healthy control sera showed reactivity to any of them...
November 1, 2017: Journal of Clinical Microbiology
Tanvir Abir, Felix Akpojene Ogbo, Garry John Stevens, Andrew Nicolas Page, Abul Hasnat Milton, Kingsley Emwinyore Agho
BACKGROUND: Appropriate antenatal care (ANC) is an important preventive public health intervention to ensure women's and newborn health outcomes. The study aimed to investigate the impact of ANC, iron-folic acid (IFA) supplementation and tetanus toxoid (TT) vaccination during pregnancy on child mortality in Bangladesh. METHOD: A cross-sectional study of three datasets from the Bangladesh Demographic and Health Surveys for the years 2004, 2007 and 2011 were pooled and used for the analyses...
2017: PloS One
Hua Dai, Haixia Zhang, Meiling Wang, Fan Ge, Ying Shen
Objective To prepare recombinant protein of human neuropilin 1 b1b2 domain (HuNRP1(b)) and monoclonal antibodies (mAbs) against the recombinant HuNRP1(b) (rHuNRP1(b)). Methods The coding sequence of HuNRP1(b) was amplified and cloned into vector pET22b to construct recombinant plasmid pET-HuNRP1(b). After analyzed by restriction enzyme digestion and DNA sequencing, pET-HuNRP1(b) was transformed into Escherichia coli and induced to express rHuNRP1(b) with histidine tag (His-HuNRP1(b)), which was identified by SDS-PAGE analysis...
September 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
Daniela Cavalleri, Martin Murphy, Wolfgang Seewald, Jason Drake, Steve Nanchen
BACKGROUND: Dogs worldwide are at risk of Babesia spp. infections. Preventive efficacy of lotilaner tablets (Credelio™, Elanco) against Babesia canis was evaluated in two studies. METHODS: Sixteen dogs in Study 1 and 12 dogs in Study 2, all seronegative and polymerase chain reaction (PCR) negative for B. canis, were randomized to a sham-treated control group or a lotilaner (20-43 mg/kg) treatment group, administered on Day 0 (Study 1: n = 8/group; Study 2: n = 6/group)...
November 1, 2017: Parasites & Vectors
Maira Salomão Fortes, Fabiana Maria Ruiz Lopes-Mori, Eloiza Teles Caldart, Caroline Constantino, Fernanda Evers, Sthefany Pagliari, Jonatas Campos de Almeida, Luiz Daniel Barros, Roberta Lemos Freire, João Luis Garcia, Selwyn Arlington Headley, Italmar Teodorico Navarro
This study investigated the prevalence of caprine toxoplasmosis in goat herds from Southern Brazil by the indirect immunofluorescence assay (IFA), and compared these results with the enzyme-linked immunosorbent assay (ELISA) and the modified agglutination test (MAT). In addition, possible risk factors associated with infection due to Toxoplasma gondii were determined. The serum samples evaluated were from 1,058 goats derived from 94 goat herds within the state of Paraná, Southern Brazil. Seropositivity by IFA was 30...
October 27, 2017: Tropical Animal Health and Production
Jian Xu, Jing Wu, Bo Jiang, Houjun He, Xixi Zhang, Xiaoyang Li, Dawei Yang, Xiufen Huang, Joshua E Sealy, Munir Iqbal, Yongqing Li
Glycoprotein D (gD) of bovine herpesvirus-1 (BoHV-1) is essential for attachment and penetration of cells during infection and is a major target for neutralizing antibodies during an adaptive immune response. Currently there are no recombinant antibodies capable of binding gD epitopes for use in treating BoHV-1 infection. In this study, a bovine scFv gene derived from a hybridoma secreting monoclonal antibodies (McAbs) against the amino acid motif MEESKGYEPP of gD was expressed in E. coli. Molecular modeling, western blot and ELISA analysis showed that this scFv had a high affinity for BoHV-1 gD, with a Kd of 161...
October 23, 2017: Applied Microbiology and Biotechnology
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