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https://www.readbyqxmd.com/read/29159199/dna-mediated-gene-therapy-in-a-mouse-model-of-limb-girdle-muscular-dystrophy-2b
#1
Julia Ma, Christophe Pichavant, Haley du Bois, Mital Bhakta, Michele P Calos
Mutations in the gene for dysferlin cause a degenerative disorder of skeletal muscle known as limb girdle muscular dystrophy 2B. To achieve gene delivery of plasmids encoding dysferlin to hind limb muscles of dysferlin knockout mice, we used a vascular injection method that perfused naked plasmid DNA into all major muscle groups of the hind limb. We monitored delivery by luciferase live imaging and western blot, confirming strong dysferlin expression that persisted over the 3-month time course of the experiment...
December 15, 2017: Molecular Therapy. Methods & Clinical Development
https://www.readbyqxmd.com/read/29158520/myd88-promotes-myoblast-fusion-in-a-cell-autonomous-manner
#2
Sajedah M Hindi, Jonghyun Shin, Yann S Gallot, Alex R Straughn, Adriana Simionescu-Bankston, Lubna Hindi, Guangyan Xiong, Robert P Friedland, Ashok Kumar
Myoblast fusion is an indispensable step for skeletal muscle development, postnatal growth, and regeneration. Myeloid differentiation primary response gene 88 (MyD88) is an adaptor protein that mediates Toll-like receptors and interleukin-1 receptor signaling. Here we report a cell-autonomous role of MyD88 in the regulation of myoblast fusion. MyD88 protein levels are increased during in vitro myogenesis and in conditions that promote skeletal muscle growth in vivo. Deletion of MyD88 impairs fusion of myoblasts without affecting their survival, proliferation, or differentiation...
November 20, 2017: Nature Communications
https://www.readbyqxmd.com/read/29156747/lethality-of-inappropriate-plasma-exposure-on-chicken-embryonic-development
#3
Jiao Jiao Zhang, Jin Oh Jo, Do Luong Huynh, Mrinmoy Ghosh, Nameun Kim, Sang Baek Lee, Hak Kyo Lee, Young Sun Mok, Taeho Kwon, Dong Kee Jeong
In this study, we examined the effects of non-thermal dielectric barrier discharge plasma on embryonic development in chicken eggs in order to determine the optimal level of plasma exposure for the promotion of embryonic growth. We exposed developing chicken embryos at either Hamburger-Hamilton (HH) stage 04 or HH 20 to plasma at voltages of 11.7 kV to 27.6 kV. Our results show exposure at 11.7 kV for 1 min promoted chicken embryonic development, but exposure to more duration and intensity of plasma resulted in dose-dependent embryonic death and HH 20 stage embryos survive longer than those at stage HH 04...
October 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/29155716/evaluation-of-injury-induced-senescence-and-in-vivo-reprogramming-in-the-skeletal-muscle
#4
Coralie Cazin, Aurelie Chiche, Han Li
Cellular senescence is a stress response that is characterized by a stable cellular growth arrest, which is important for many physiological and pathological processes, such as cancer and ageing. Recently, senescence has also been implicated in tissue repair and regeneration. Therefore, it has become increasingly critical to identify senescent cells in vivo. Senescence-associated β-galactosidase (SA-β-Gal) assay is the most widely used assay to detect senescent cells both in culture and in vivo. This assay is based on the increased lysosomal contents in the senescent cells, which allows the histochemical detection of lysosomal β-galactosidase activity at suboptimum pH (6 or 5...
October 26, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29147013/integrin-alpha-11-in-the-regulation-of-the-myofibroblast-phenotype-implications-for-fibrotic-diseases
#5
Ruchi Bansal, Shigeki Nakagawa, Saleh Yazdani, Joop van Baarlen, Anu Venkatesh, Anna P Koh, Won-Min Song, Nicolas Goossens, Hideo Watanabe, Mary B Beasley, Charles A Powell, Gert Storm, Naftali Kaminski, Harry van Goor, Scott L Friedman, Yujin Hoshida, Jai Prakash
Tissue fibrosis, characterized by excessive accumulation of aberrant extracellular matrix (ECM) produced by myofibroblasts, is a growing cause of mortality worldwide. Understanding the factors that induce myofibroblastic differentiation is paramount to prevent or reverse the fibrogenic process. Integrin-mediated interaction between the ECM and cytoskeleton promotes myofibroblast differentiation. In the present study, we explored the significance of integrin alpha 11 (ITGA11), the integrin alpha subunit that selectively binds to type I collagen during tissue fibrosis in the liver, lungs and kidneys...
November 17, 2017: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/29146735/reduced-dnmt3a-increases-gdf5-expression-with-suppressed-satellite-cell-differentiation-and-impaired-skeletal-muscle-regeneration
#6
Yukino Hatazawa, Yusuke Ono, Yuma Hirose, Sayaka Kanai, Nobuharu L Fujii, Shuichi Machida, Ichizo Nishino, Takahiko Shimizu, Masaki Okano, Yasutomi Kamei, Yoshihiro Ogawa
DNA methylation is an epigenetic mechanism regulating gene expression. In this study, we observed that DNA methyltransferase 3a (Dnmt3a) expression is decreased after muscle atrophy. We made skeletal muscle-specific Dnmt3a knockout mice (Dnmt3a-KO mice). The regeneration capacity after muscle injury was markedly decreased in Dnmt3a-KO mice. Diminished mRNA and protein expression of Dnmt3a were observed in skeletal muscles as well as in satellite cells, which are important for muscle regeneration, in Dnmt3a-KO mice...
November 16, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29143982/tweak-fn14-interaction-induces-proliferation-and-migration-in-human-airway-smooth-muscle-cells-via-activating-the-nf-%C3%AE%C2%BAb-pathway
#7
Cuimin Zhu, Leguo Zhang, Zhiming Liu, Chen Li, Yajie Bai
Asthma, an increasingly common chronic disease among children, are characterized by airway remodeling, which is partly attributed to the proliferation and migration of airway smooth muscle cell (ASMC). The purpose of the present study was to investigate potential roles and mechanisms of the tumor necrosis factor-like weak inducer of apoptosis (TWEAK)/fibroblast growth factor-inducible molecule 14 (Fn14) axis on cell proliferation and migration in HASMCs. Compared to HASMCs from non-asthmatic patients, those from asthmatic patients showed elevated expression levels of both Fn14 and TWEAK...
November 16, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29142264/impairment-of-bkca-channels-in-human-placental-chorionic-plate-arteries-is-potentially-relevant-to-the-development-of-preeclampsia
#8
Mengzhou He, Fanfan Li, Meitao Yang, Yao Fan, Rajluxmee Beejadhursing, Yin Xie, Yuan Zhou, Dongrui Deng
Preeclampsia has known associations with insufficient placental perfusion. The large-conductance Ca(2+)-activated K(+) (BKca) channels that have recently been found to play important roles in cellular growth and vasodilatation could potentially participate in the development of preeclampsia. However, the mechanisms by which downregulated BKca channels are involved in the development of preeclampsia remain unknown. In this study, we investigated the mechanism(s) underlying the impairment of vascular tone regulation by BKca channels in human placental chorionic plate arteries (CPAs) in preeclampsia...
November 16, 2017: Hypertension Research: Official Journal of the Japanese Society of Hypertension
https://www.readbyqxmd.com/read/29140133/ite-inhibits-growth-of-human-pulmonary-artery-endothelial-cells
#9
Ling-Pin Pang, Yan Li, Qing-Yun Zou, Chi Zhou, Wei Lei, Jing Zheng, Shi-An Huang
AIM: Pulmonary arterial hypertension (PAH), a deadly disorder is associated with excessive growth of human pulmonary artery endothelial (HPAECs) and smooth muscle (HPASMCs) cells. Current therapies primarily aim at promoting vasodilation, which only ameliorates clinical symptoms without a cure. 2-(1'H-indole-3'-carbonyl)-thiazole-4-carboxylic acid methyl ester (ITE) is an endogenous aryl hydrocarbon receptor (AhR) ligand, and mediates many cellular function including cell growth. However, the roles of ITE in human lung endothelial cells remain elusive...
October 2017: Experimental Lung Research
https://www.readbyqxmd.com/read/29133788/regulation-of-angiotensin-ii-actions-by-enhancers-and-super-enhancers-in-vascular-smooth-muscle-cells
#10
Sadhan Das, Parijat Senapati, Zhuo Chen, Marpadga A Reddy, Rituparna Ganguly, Linda Lanting, Varun Mandi, Anita Bansal, Amy Leung, Selena Zhang, Ye Jia, Xiwei Wu, Dustin E Schones, Rama Natarajan
Angiotensin II (AngII) promotes hypertension and atherosclerosis by activating growth-promoting and pro-inflammatory gene expression in vascular smooth muscle cells (VSMCs). Enhancers and super-enhancers (SEs) play critical roles in driving disease-associated gene expression. However, enhancers/SEs mediating VSMC dysfunction remain uncharacterized. Here, we show that AngII alters vascular enhancer and SE repertoires in cultured VSMCs in vitro, ex vivo, and in AngII-infused mice aortas in vivo. AngII-induced enhancers/SEs are enriched in binding sites for signal-dependent transcription factors and dependent on key signaling kinases...
November 13, 2017: Nature Communications
https://www.readbyqxmd.com/read/29123487/hypertrophy-stimulation-at-the-onset-of-type-i-diabetes-maintains-the-soleus-but-not-the-edl-muscle-mass-in-wistar-rats
#11
Marco A S Fortes, Maria V M Scervino, Gabriel N Marzuca-Nassr, Kaio F Vitzel, Carlos H da Justa Pinheiro, Rui Curi
Diabetes mellitus induces a reduction in skeletal muscle mass and strength. Strength training is prescribed as part of treatment since it improves glycemic control and promotes increase of skeletal muscle mass. The mechanisms involved in overload-induced muscle hypertrophy elicited at the establishment of the type I diabetic state was investigated in Wistar rats. The purpose was to examine whether the overload-induced hypertrophy can counteract the hypotrophy associated to the diabetic state. The experiments were performed in oxidative (soleus) or glycolytic (EDL) muscles...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/29121593/stimulation-of-liver-igf-1-expression-promotes-peak-bone-mass-achievement-in-growing-rats-a-study-with-pomegranate-seed-oil
#12
Deepa Bachagol, Gilbert Stanley Joseph, Govindraj Ellur, Kalpana Patel, Pamisetty Aruna, Monika Mittal, Shyamsundar Pal China, Ravendra Pratap Singh, Kunal Sharan
Peak bone mass (PBM) achieved at adulthood is a strong determinant of future onset of osteoporosis, and maximizing it is one of the strategies to combat the disease. Recently, pomegranate seed oil (PSO) has been shown to have bone-sparing effect in ovariectomized mice. However, its effect on growing skeleton and its molecular mechanism remain unclear. In the present study, we evaluated the effect of PSO on PBM in growing rats and associated mechanism of action. PSO was given at various doses to 21-day-old growing rats for 90 days by oral gavage...
October 12, 2017: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/29119737/endostatin-inhibits-fibrosis-by-modulating-the-pdgfr-erk-signal-pathway-an-in-vitro-study
#13
Yuan Li, Hai-Tao Ren
Accumulating evidence indicates that endostatin inhibits fibrosis. However, the mechanism is yet to be clarified. The aim of this study is to evaluate the effect of endostatin on platelet-derived growth factor-BB (PDGF-BB)- or transforming growth factor β1 (TGF-β1)-induced fibrosis in cultured human skin fibroblasts, and to further examine the molecular mechanisms involved. Human dermal fibroblasts were cultured in Dulbecco's modified Eagle's medium (DMEM) and serum-starved for 48 h before treatment. Cells were grouped as follows: "PDGF-BB", "PDGF-BB+ endostatin", "TGF-β1", "TGF-β1+endostatin", "endostatin", and "blank control"...
2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/29119332/therapeutic-effect-of-vinorine-on-sciatic-nerve-injured-rat
#14
Dongdong Guo, Xingang Lu, Xiaoli Xu, Haixin Gou, Zhiqian Wang, Yi Cao, Xingjing Luo
Vinorine is a monoterpenoid indole alkaloid, a type of natural alkaloids. Growing reports exhibited the numerous pharmacology activities of vinorine such as anti-inflammation, anti-bacterial and anti-tumor. In this study, the effect of vinorine injection (7.5, 15 and 30 mg/kg) on motor function, sensation and nerve regeneration in sciatic nerve crush injury rat was investigated. The results of behavioral analysis, electrophysiological analysis and muscle histological analysis suggested that vinorine promoted the motor function recovery after sciatic nerve injury...
November 8, 2017: Neurochemical Research
https://www.readbyqxmd.com/read/29118903/mir-665-regulates-vsmcs-proliferation-via-targeting-fgf9-and-mef2d-and-modulating-activities-of-wnt-%C3%AE-catenin-signaling
#15
Kai Li, Jin Pan, Jianjun Wang, Fengrui Liu, Li Wang
Abnormal proliferation of vascular smooth muscle cells (VSMCs) contributes to the development of cardiovascular diseases. Studies have showed the great impact of microRNAs (miRNAs) on the cell proliferation in VSMCs. This study examined the in vitro functional roles of miR-665 in the VSMCs and explored the underlying molecular mechanisms. The mRNA and protein expression levels were determined by qRT-PCR and western blot assays, respectively. CCK-8, transwell invasion and wound healing assays were performed to measure VSMCs proliferation, invasion and migration, respectively...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/29117616/invasive-pituitary-adenoma-derived-tumor-associated-fibroblasts-promote-tumor-progression-both-in-vitro-and-in-vivo
#16
Liang Lv, Shizhen Zhang, Yu Hu, Peizhi Zhou, Ling Gao, Mengmeng Wang, Zhen Sun, Cheng Chen, Senlin Yin, Xiujie Wang, Shu Jiang
Tumor-associated fibroblasts are the most abundant population in tumor stroma and impact on tumor initiation and progression. However, the biological function of tumor-associated fibroblasts in pituitary adenomas has not been fully elucidated to date. So, this study aims to clarify the function and significance of primary cultured pituitary adenoma-derived tumor-associated fibroblasts on rat pituitary adenoma cells. We identified primary cultured tumor-associated fibroblasts and normal fibroblasts based on the expression of α-smooth muscle actin as well as morphology...
November 8, 2017: Experimental and Clinical Endocrinology & Diabetes
https://www.readbyqxmd.com/read/29114532/changes-in-physical-fitness-and-body-composition-according-to-the-physical-activities-of-korean-adolescents
#17
Minsoo Cho, Ji-Youn Kim
This study comparatively analyzed obesity, lean balance, and physical fitness related to growth and development in female middle school students to investigate the importance of regular physical activity on body composition and physical fitness in adolescence. The subjects were 31 elementary school students in grades 5-6 in Seoul, consisting of 16 students in the regularly active group and 15 students in the inactive group. Body composition and physical fitness factors were measured. Height, weight, body mass index (BMI), fat mass, and fat-free mass were measured using Inbody 3...
October 2017: Journal of Exercise Rehabilitation
https://www.readbyqxmd.com/read/29114002/sdf-1%C3%AE-stromal-cell-derived-factor-1%C3%AE-induces-cardiac-fibroblasts-renal-microvascular-smooth-muscle-cells-and-glomerular-mesangial-cells-to-proliferate-cause-hypertrophy-and-produce-collagen
#18
Edwin K Jackson, Yumeng Zhang, Delbert D Gillespie, Xiao Zhu, Dongmei Cheng, Travis C Jackson
BACKGROUND: Activated cardiac fibroblasts (CFs), preglomerular vascular smooth muscle cells (PGVSMCs), and glomerular mesangial cells (GMCs) proliferate, cause hypertrophy, and produce collagen; in this way, activated CFs contribute to cardiac fibrosis, and activated PGVSMCs and GMCs promote renal fibrosis. In heart and kidney diseases, SDF-1α (stromal cell-derived factor 1α; endogenous CXCR4 [C-X-C motif chemokine receptor 4] receptor agonist) levels are often elevated; therefore, it is important to know whether and how the SDF-1α/CXCR4 axis activates CFs, PGVSMCs, or GMCs...
November 7, 2017: Journal of the American Heart Association
https://www.readbyqxmd.com/read/29111780/e26-transformation-specific-transcription-factor-ets2-as-an-oncogene-promotes-the-progression-of-hypopharyngeal-cancer
#19
Xuejun Liu, Chuqin Zhang, Zhonghua Zhang, Zuping Zhang, Wei Ji, Shengda Cao, Xiaolan Cai, Dapeng Lei, Xinliang Pan
The E26 transformation-specific (ETS) family is one of the largest families of transcription factors. Upon activation by MAPK pathway, ETS participates in cell proliferation, differentiation, migration, apoptosis, and metastasis. However, the mechanism by which ETS is deregulated in cancer is unclear. In this study, the authors investigated the role of ETS factor, ETS2, in hypopharyngeal cancer pathogenesis in hypopharyngeal cancer tissues (N = 20) and corresponding non-neoplastic tissues (N = 20). The results showed that expression of ETS2 was increased in cancer tissues as compared with the expression in corresponding non-neoplastic tissues...
November 2017: Cancer Biotherapy & Radiopharmaceuticals
https://www.readbyqxmd.com/read/29109273/blockade-of-activin-type-ii-receptors-with-a-dual-anti-actriia-iib-antibody-is-critical-to-promote-maximal-skeletal-muscle-hypertrophy
#20
Frederic Morvan, Jean-Michel Rondeau, Chao Zou, Giulia Minetti, Clemens Scheufler, Meike Scharenberg, Carsten Jacobi, Pascale Brebbia, Veronique Ritter, Gauthier Toussaint, Claudia Koelbing, Xavier Leber, Alain Schilb, Florian Witte, Sylvie Lehmann, Elke Koch, Sabine Geisse, David J Glass, Estelle Lach-Trifilieff
The TGF-β family ligands myostatin, GDF11, and activins are negative regulators of skeletal muscle mass, which have been reported to primarily signal via the ActRIIB receptor on skeletal muscle and thereby induce muscle wasting described as cachexia. Use of a soluble ActRIIB-Fc "trap," to block myostatin pathway signaling in normal or cachectic mice leads to hypertrophy or prevention of muscle loss, perhaps suggesting that the ActRIIB receptor is primarily responsible for muscle growth regulation. Genetic evidence demonstrates however that both ActRIIB- and ActRIIA-deficient mice display a hypertrophic phenotype...
November 6, 2017: Proceedings of the National Academy of Sciences of the United States of America
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