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https://www.readbyqxmd.com/read/29785122/aloperine-executes-antitumor-effects-through-the-induction-of-apoptosis-and-cell-cycle-arrest-in-prostate-cancer-in-vitro-and-in-vivo
#1
Zhixin Ling, Han Guan, Zonghao You, Can Wang, Ling Hu, Lei Zhang, Yiduo Wang, Shuqiu Chen, Bin Xu, Ming Chen
Background: Prostate cancer (PCa) is one of the most common malignant diseases among male patients. Although androgen deprivation therapy remains the main treatment for PCa, most patients would inevitably progress to castration-resistant PCa, which is the main cause of cancer-related deaths. Thus, novel antitumor agents are urgently needed. Recent studies demonstrated that aloperine (ALO) as a natural alkaloid showed antitumor effects in other cancer types. However, the biological function and underlying mechanisms of ALO in PCa have not been investigated...
2018: OncoTargets and Therapy
https://www.readbyqxmd.com/read/29748961/growth-hormone-releasing-hormone-receptor-antagonists-modify-molecular-machinery-in-the-progression-of-prostate-cancer
#2
Laura Muñoz-Moreno, Andrew V Schally, Juan C Prieto, M José Carmena, Ana M Bajo
BACKGROUND: Therapeutic strategies should be designed to transform aggressive prostate cancer phenotypes to a chronic situation. To evaluate the effects of the new growth hormone-releasing hormone receptor (GHRH-R) antagonists: MIA-602, MIA-606, and MIA-690 on processes associated with cancer progression as cell proliferation, adhesion, migration, and angiogenesis. METHODS: We used three human prostate cell lines (RWPE-1, LNCaP, and PC3). We analyzed several molecules such as E-cadherin, β-catenin, Bcl2, Bax, p53, MMP2, MMP9, PCNA, and VEGF and signaling mechanisms that are involved on effects exerted by GHRH-R antagonists...
May 10, 2018: Prostate
https://www.readbyqxmd.com/read/29629344/esculetin-inhibits-the-survival-of-human-prostate-cancer-cells-by-inducing-apoptosis-and-arresting-the-cell-cycle
#3
Kader Turkekul, R Dilsu Colpan, Talha Baykul, Mehmet D Ozdemir, Suat Erdogan
Background: Prostate cancer (PCa) is one of the most important causes of death in men and thus new therapeutic approaches are needed. In this study, antiproliferative and anti-migration properties of a coumarin derivative esculetin were evaluated. Methods: Human PCa cell lines PC3, DU145, and LNCaP were treated with various concentrations of esculetin for 24 to 72 hours, and cell viability was determined by the MTT test. Cell cycle and apoptosis were analyzed by using cell-based cytometer...
March 2018: Journal of Cancer Prevention
https://www.readbyqxmd.com/read/29622769/alterations-in-the-p53-socs2-axis-contribute-to-tumor-growth-in-colon-cancer
#4
Jong-Hwan Kim, Mi-Jin Lee, Goung-Ran Yu, Sang-Wook Kim, Kyu-Yun Jang, Hee-Chul Yu, Baik-Hwan Cho, Dae-Ghon Kim
Altered expression of suppressor of cytokine signaling (SOCS) is found in various tumors. However, regulation of SOCS2 by upstream molecules has yet to be clearly elucidated, particularly in tumor cells. SCOCS2 expression was examined in tumor cells transfected with an inducible p53 expression system. The impact of SOCS2 on cell proliferation was measured with in vitro assays. Inhibition of tumorigenicity by SOCS2 knockdown was assessed via a mouse model. Expression profiles were compared and genes differentially expressed were identified using four types of p53-null cells (Saos, HLK3, PC3, and H1299) and the same cells stably expressing p53...
April 6, 2018: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/29620284/enrichment-and-mutation-detection-of-circulating-tumor-cells-from-blood-samples
#5
Ruqin Kou, Jian Zhao, Priya Gogoi, Shannon Carskadon, Will Chow, Clara Hwang, Nallasivam Palanisamy, Conrad Leung, Yixin Wang
The potential of circulating tumor cells (CTCs) in the diagnosis and prognosis of cancer patients has become increasingly attractive. However, molecular analysis of CTCs is hindered by low sensitivity and a high level of background leukocytes in CTC enrichment technologies. We have developed a novel protocol using a microfluidic device, which enriches and retrieves CTCs from blood samples. The principle of CTC capturing is that tumor cells are larger and less deformable than normal blood cells. To evaluate the potential of utilizing Celsee PREP100 in CTC molecular analysis, we prepared prostate cancer cell lines PC3 and LNCaP, retrieved the captured cells and analyzed them using PCR amplicon sequencing...
March 30, 2018: Oncology Reports
https://www.readbyqxmd.com/read/29573707/a-novel-tetrahydroisoquinoline-thiq-analogue-induces-mitochondria-dependent-apoptosis
#6
Xia Sun, Min Liu, Luyao Gao, Ying Mao, Diming Zhao, Jianfeng Zhuang, Lei Liu
Lung cancer continues to be a leading cause of cancer-related death worldwide, with non-small cell lung cancer (NSCLC) accounting for more than 80% of lung cancer cases. Current therapies for NSCLC have only limited effect and treatment resistance develops rapidly. In a previous study, we have shown that C1 -phenylethynyl tetrahydroisoquinoline (THIQ) analogue 4 has anti-proliferative activity against PC3 human prostate cancer cells. However, this anticancer effect was achieved with relatively high IC50 in A549 lung cancer cells...
March 7, 2018: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29549167/tetrandrine-tet-induces-death-receptors-apo-trail-r1-dr4-and-apo-trail-r2-dr5-and-sensitizes-prostate-cancer-cells-to-trail-induced-apoptosis
#7
Gauri Shishodia, Sweaty Koul, Qin Dong, Hari K Koul
TNF-related apoptosis-inducing ligand (TRAIL) selectively induces apoptosis in cancer cells, but not in normal cells, as such is a promising therapeutic agent. However, therapeutic resistance limits its clinical use in many malignancies including prostate cancer (PCa). Strategies to sensitize cancer cells to TRAIL are urgently needed. We demonstrate here that small-molecule Tetrandrine (TET) potentially sensitizes previously resistant (LNCaP and C4-2B cells) and mildly sensitive (PC3 cells) PCa cells to TRAIL- induced apoptosis, and they do so by up-regulating mRNA expression and protein levels of death receptors Apo Trail R1 (DR4) and Apo Trail R2 (DR5)...
March 16, 2018: Molecular Cancer Therapeutics
https://www.readbyqxmd.com/read/29475156/potent-hydrazone-derivatives-targeting-esophageal-cancer-cells
#8
Ling-Yu Li, Jia-Di Peng, Wenjuan Zhou, Hui Qiao, Xin Deng, Zhou-Hua Li, Ji-Deng Li, Yun-Dong Fu, Song Li, Kai Sun, Hong-Min Liu, Wen Zhao
Hydrazone and their derivatives are a series of highly active molecules, which are widely used as lead compounds for the research and development of new anti-cancer drugs. In this study, 20 compounds were synthesized, based on this scaffold and their in vitro cytotoxicity against 6 cancer cell lines, including EC9706, SMMC-7721, MCF7, PC3, MGC-803 and EC109 was tested. Among them, compound 6p, showed strong anti-proliferative activities on esophageal carcinoma cells: EC9706 and EC109 with IC50 values of 1...
March 25, 2018: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28918838/inhibition-of-midkine-suppresses-prostate-cancer-cd133-stem-cell-growth-and-migration
#9
Suat Erdogan, Zeynep B Doganlar, Oguzhan Doganlar, Kader Turkekul, Riza Serttas
BACKGROUND: Midkine (MDK) is a tumor-promoting factor that is often overexpressed in various human carcinomas, and the role of MDK has not yet been fully investigated in prostate cancer stem cells. MATERIALS AND METHODS: Prostate cancer CD133+ stem cells (PCSCs) were isolated from human castration-resistant PC3 cells. PCSCs were treated with different concentrations of MDK inhibitor, iMDK, for 24-72 hours. The IC50 values were determined by the MTT test. Endogenous MDK messenger RNA expression was knocked down by small interfering RNA...
September 2017: American Journal of the Medical Sciences
https://www.readbyqxmd.com/read/28869219/andrographolide-sensitizes-prostate-cancer-cells-to-trail-induced-apoptosis
#10
Ruo-Jing Wei, Xin-Shi Zhang, Da-Lin He
Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a promising agent for anticancer therapy. The identification of small molecules that can establish the sensitivity of prostate cancer (PCa) cells to TRAIL-induced apoptosis is crucial for the targeted treatment of PCa. PC3, DU145, JAC-1, TsuPr1, and LNCaP cells were treated with Andrographolide (Andro) and TRAIL, and the apoptosis was measured using the Annexin V/PI double staining method. Real time-polymerase chain reaction (PCR) and Western blot analysis were performed to measure the expression levels of target molecules...
March 2018: Asian Journal of Andrology
https://www.readbyqxmd.com/read/28846663/chemopreventive-potential-of-ethanolic-extracts-of-luobuma-leaves-apocynum-venetum-l-in-androgen-insensitive-prostate-cancer
#11
Szu-Ping Huang, Tzu-Ming Ho, Chih-Wen Yang, Ya-Ju Chang, Jie-Fu Chen, Ning-Sing Shaw, Jia-Cherng Horng, Shih-Lan Hsu, Ming-Yuan Liao, Li-Chen Wu, Ja-An Annie Ho
Luobuma (Apocynum venetum L. (AVL)) is a popular beverage in Asia and has been reportedly to be associated with the bioactivities such as cardiotonic, diuretic, antioxidative, and antihypertensive. However, its biofunction as chemoprevention activity is seldom addressed. Herein, we aimed to characterize the anti-androgen-insensitive-prostate-cancer (anti-AIPC) bioactive compounds of Luobuma, and to investigate the associated molecular mechanisms. Activity-guided-fractionation (antioxidative activity and cell survivability) of Luobuma ethanolic extracts was performed to isolate and characterize the major bioactive compounds using Ultra Performance Liquid Chromatography (UPLC), Liquid Chromatography-Mass Spectrometry (LC-MS), and Nuclear Magnetic Resonance (NMR)...
August 28, 2017: Nutrients
https://www.readbyqxmd.com/read/28765552/antitumoral-effects-of-%C3%AE-cdcpli-a-pla2-inhibitor-from-crotalus-durissus-collilineatus-via-pi3k-akt-pathway-on-mda-mb-231-breast-cancer-cell
#12
Sarah N C Gimenes, Daiana S Lopes, Patrícia T Alves, Fernanda V P V Azevedo, Lara Vecchi, Luiz R Goulart, Thais C S Rodrigues, André L Q Santos, Vera L de C Brites, Thaise L Teixeira, Cláudio V da Silva, Matheus H Dias, Samuel C Teixeira, Renata S Rodrigues, Kelly A G Yoneyama, Ricardo A Oliveira, Veridiana de M Rodrigues
Phospholipases A2 (PLA2s) overexpression is closely associated with the malignant potential of breast cancers. Here, we showed for the first the antitumoral effects of γCdcPLI, a PLA2 inhibitor from Crotalus durissus collilineatus via PI3K/Akt pathway on MDA-MB-231 cell. Firstly, γCdcPLI was more cytotoxic to MDA-MB-231 breast cancer cells than other cell lines (MCF-7, HeLa, PC3 and A549) and did not affect the viability of non-tumorigenic breast cell (MCF 10A). In addition, γCdcPLI induced modulation of important mediators of apoptosis pathways such as p53, MAPK-ERK, BIRC5 and MDM2...
August 1, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28624236/regulation-of-the-proprotein-convertases-expression-and-activity-during-regenerative-angiogenesis-role-of-hypoxia-inducible-factor-hif
#13
Jia Ma, Serge Evrard, Iker Badiola, Geraldine Siegfried, Abdel-Majid Khatib
Proprotein convertases (PCs) are involved in various physiological and pathological processes ranging from embryogenesis to carcinogenesis. Here, using the zebrafish fin regeneration model, we report induced expression of furin and PC5 but not PACE4 and PC7 during fin regeneration that is associated with increased PC activity. Stabilization of HIF by cobalt chloride (CoCl2) further increases these processes. The use of the general PC-inhibitor decanoyl-RVKR-cholromethyl ketone (CMK) inhibited control and CoCl2 -induced PC activity...
August 2017: European Journal of Cell Biology
https://www.readbyqxmd.com/read/28551639/a-novel-near-infrared-fluorescent-protein-irfp720-facilitates-transcriptional-profiling-of-prostate-cancer-bone-metastasis-in-mice
#14
Mariko Honda, Satomi Yogosawa, Minori Kamada, Yuko Kamata, Takahiro Kimura, Yusuke Koike, Toru Harada, Hiroyuki Takahashi, Shin Egawa, Kiyotsugu Yoshida
BACKGROUND: Bone represents a frequent site of prostate cancer metastasis. As the molecular mechanism remains unclear, an accessible animal model is required. MATERIALS AND METHODS: We established a novel murine metastasis model using near-infrared fluorescent protein iRFP720-labelled prostate cancer (PC3) cells. To clarify transcriptional alterations during metastasis, iRFP720-PC3 cells were intracardially injected into male mice. mRNA expression profiles of metastasis in bone using marrow cancer cells extracted by centrifugal separation and cell sorting were compared with those of parental cells by microarray...
June 2017: Anticancer Research
https://www.readbyqxmd.com/read/28525372/overexpression-of-a-novel-candidate-oncogene-kif14-correlates-with-tumor-progression-and-poor-prognosis-in-prostate-cancer
#15
Yixiang Zhang, Yeqing Yuan, Pei Liang, Zhaoxia Zhang, Xiaojing Guo, Ligang Xia, Yingying Zhao, Xing-Sheng Shu, Shengkun Sun, Ying Ying, Yingduan Cheng
Prostate cancer (PCa) is the second leading cause of death from cancer in men. The mechanism underlying tumorigenesis and development of PCa is largely unknown. Here, we identified Kinesin family member 14 (KIF14) as a novel candidate oncogene in PCa. We found that KIF14 was overexpressed in multiple PCa cell lines and primary PCa tissues. Knockdown of KIF14 in DU145 and PC3 prostate cancer cells suppressed cell proliferation, induced cell cycle arrest and apoptosis. Transcriptome analysis by RNA-sequencing demonstrated that KIF4 suppression led to transcriptional changes of genes involved in p53 and TGF-beta signaling pathway...
July 11, 2017: Oncotarget
https://www.readbyqxmd.com/read/28485104/microrna-181a-promotes-docetaxel-resistance-in-prostate-cancer-cells
#16
Cameron M Armstrong, Chengfei Liu, Wei Lou, Alan P Lombard, Christopher P Evans, Allen C Gao
BACKGROUND: Docetaxel is one of the primary drugs used for treating castration resistant prostate cancer (CRPC). Unfortunately, over time patients invariably develop resistance to docetaxel therapy and their disease will continue to progress. The mechanisms by which resistance develops are still incompletely understood. This study seeks to determine the involvement of miRNAs, specifically miR-181a, in docetaxel resistance in CRPC. METHODS: Real-time PCR was used to measure miR-181a expression in parental and docetaxel resistant C4-2B and DU145 cells (TaxR and DU145-DTXR)...
June 2017: Prostate
https://www.readbyqxmd.com/read/28454475/oridonin-induces-g2-m-cell-cycle-arrest-and-apoptosis-via-the-pi3k-akt-signaling-pathway-in-hormone-independent-prostate-cancer-cells
#17
Jianlei Lu, Xiang Chen, Shuang Qu, Bing Yao, Yuexin Xu, Jiahui Wu, Yucui Jin, Changyan Ma
Oridonin is an active constituent isolated from the traditional Chinese herb Rabdosia rubescens, which exerts antitumor effects in experimental and clinical settings. However, its antitumor effects and underlying mechanisms on prostate cancer cells have not yet been clearly identified. In the present study, the androgen-independent prostate cancer PC3 and DU145 cell lines were used as models to investigate the effects and possible mechanisms of oridonin on cellular proliferation and apoptosis. Results demonstrated that oridonin inhibited cellular proliferation, and was able to significantly induce G2/M cell cycle arrest and apoptosis...
April 2017: Oncology Letters
https://www.readbyqxmd.com/read/28259690/the-herbal-medicine-melissa-officinalis-extract-effects-on-gene-expression-of-p53-bcl-2-her2-vegf-a-and-htert-in-human-lung-breast-and-prostate-cancer-cell-lines
#18
Rana Jahanban-Esfahlan, Khaled Seidi, Amir Monfaredan, Vahid Shafie-Irannejad, Mehran Mesgari Abbasi, Ansar Karimian, Bahman Yousefi
INTRODUCTION: Earlier, we verified that Melissa officinalis extract (MOE) elicits potent antiproliferative effects on different human cancer cells. To gain insights into the molecular mechanisms accounting for the cytotoxic effects of MOE, we assessed the expression patterns of several prominent molecules with therapeutic potential in cancer by Quantitative PCR (Q-PCR). METHODS: A549, MCF-7 and PC3 cancer cells were grown in complete RPMI 1640 and seeded in 24 well micro plates...
May 20, 2017: Gene
https://www.readbyqxmd.com/read/28242062/synthesis-and-topoisomerase-i-inhibitory-properties-of-klavuzon-derivatives
#19
İsmail Akçok, Derya Mete, Ayhan Şen, Pınar Kasaplar, Kemal S Korkmaz, Ali Çağır
Klavuzon is a naphthalen-1-yl substituted α,β-unsaturated δ-lactone derivative, and is one of the anti-proliferative members of this class of compounds. Asymmetric and racemic syntheses of novel α,β-unsaturated δ-lactone derivatives are important to investigate their potential for the treatment of cancer. In this study, asymmetric and racemic syntheses of heteroatom-substituted klavuzon derivatives are reported. The syntheses were completed by a well-known three-step procedure. Anti-proliferative activity of seven novel racemic klavuzon derivatives were reported against MCF-7, PC3, HCT116 p53+/+ and HCT116 p53-/- cancer cell lines...
April 2017: Bioorganic Chemistry
https://www.readbyqxmd.com/read/28199187/combinatorial-effect-of-curcumin-with-docetaxel-modulates-apoptotic-and-cell-survival-molecules-in-prostate-cancer
#20
Saswati Banerjee, Santosh K Singh, Indrajit Chowdhury, James W Lillard, Rajesh Singh
Docetaxel is the most commonly used chemotherapeutic agent to target androgen signaling in metastatic prostate cancer (PCa); however, prolonged treatment with docetaxel results in drug-resistant cancer cells. Combination therapies have the potential of increasing the effectiveness of drug treatment as well as decreasing the side effects. Curcumin is a nontoxic organic compound with multifaceted chemopreventive potential. In this study, we evaluated whether curcumin can reinforce the effect of docetaxel on PCa cells...
March 1, 2017: Frontiers in Bioscience (Elite Edition)
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