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Christian wadsack

J Loegl, U Hiden, E Nussbaumer, C Schliefsteiner, S Cvitic, I Lang, C Wadsack, B Huppertz, G Desoye
The human placenta comprises a special type of tissue macrophages, the Hofbauer cells (HBC), which exhibit M2 macrophage phenotype. Several subtypes of M2-polarized macrophages (M2a, M2b and M2c) exist in almost all tissues. Macrophage polarization depends on the way of macrophage activation and leads to the expression of specific cell surface markers and the acquisition of specific functions, including tissue remodeling and the promotion of angiogenesis. The placenta is a highly vascularized and rapidly growing organ, suggesting a role of HBC in feto-placental angiogenesis...
November 2016: Reproduction: the Official Journal of the Society for the Study of Fertility
Christian Löffler, Horst Sattler, Lena Peters, Anika Tuleweit, Uta Löffler, Daniel Wadsack, Michael Uppenkamp, Raoul Bergner
OBJECTIVES: Power Doppler ultrasound is used to assess joint vascularity in acute arthritis. PDUS signals have been correlated with synovial histology and bone deterioration. Little is known about the correlation between power Doppler signals and synovial white blood count. In our study, we analyzed power Doppler signals in inflammatory joint diseases including gout, calcium pyrophosphate deposition disease, rheumatoid arthritis, spondyloarthritis and others and correlated power Doppler signals with synovial white blood count and with serologic markers of inflammation...
October 2016: Joint, Bone, Spine: Revue du Rhumatisme
Carolin Besenboeck, Silvija Cvitic, Uwe Lang, Gernot Desoye, Christian Wadsack
The phospholipase A2 (PLA2) family is a very diverse group of enzymes, all serving in the cleavage of phospholipids, thereby releasing high amounts of arachidonic acid (AA) and lysophospholipids. AA serves as a substrate for prostaglandin production, which is of special importance in pregnancy for the onset of parturition. Novel research demonstrates that PLA2 action affects the immune response of the mother toward the child and is therefore probably implied in the tolerance of the fetus and prevention of miscarriage...
June 2016: Reproduction: the Official Journal of the Society for the Study of Fertility
Eva Bernhart, Sabine Damm, Andrea Wintersperger, Christoph Nusshold, Anna Martina Brunner, Ioanna Plastira, Gerald Rechberger, Helga Reicher, Christian Wadsack, Andreas Zimmer, Ernst Malle, Wolfgang Sattler
Glioblastoma is the most common malignant brain tumor, which, despite combined radio- and chemotherapy, recurs and is invariably fatal for affected patients. Members of the sphingolipid (SL) family are potent effectors of glioma cell proliferation. In particular sphingosine-1-phosphate (S1P) and the corresponding G protein-coupled S1P receptors transmit proliferative signals to glioma cells. To investigate the contribution to glioma cell proliferation we inhibited the first step of de novo SL synthesis in p53(wt) and p53(mut) glioma cells, and interfered with S1P signaling specifically in p53(wt) U87MG cells...
July 15, 2015: Biochemical Pharmacology
Ivana Sreckovic, Ruth Birner-Gruenberger, Carolin Besenboeck, Milica Miljkovic, Tatjana Stojakovic, Hubert Scharnagl, Gunther Marsche, Uwe Lang, Jelena Kotur-Stevuljevic, Zorana Jelic-Ivanovic, Gernot Desoye, Christian Wadsack
Gestational diabetes mellitus (GDM) is related to neonatal macrosomia and an increased risk of vascular events. We hypothesized that GDM exerts qualitative effects on neonatal high-density lipoprotein (HDL). HDL was isolated from control (n=11) and GDM maternal/neonatal donors (n=9) and subjected to shotgun proteomics. Differences in HDL mobility were assessed by FPLC and native gel-electrophoresis. Paraoxonase (PON1) activity, cholesterol ester-transfer protein (CETP) mass and activity, phospholipid, triglyceride and cholesterol concentrations were quantified with commercial kits...
November 2014: Biochimica et Biophysica Acta
Dalia El-Gamal, Shailaja P Rao, Michael Holzer, Seth Hallström, Johannes Haybaeck, Martin Gauster, Christian Wadsack, Andrijana Kozina, Saša Frank, Rudolf Schicho, Rufina Schuligoi, Akos Heinemann, Gunther Marsche
The dramatic cardiovascular mortality of patients with chronic kidney disease is attributable in a significant proportion to endothelial dysfunction. Cyanate, a reactive species in equilibrium with urea, is formed in excess in chronic kidney disease. Cyanate is thought to have a causal role in promoting cardiovascular disease, but the underlying mechanisms remain unclear. Immunohistochemical analysis performed in the present study revealed that carbamylated epitopes associate mainly with endothelial cells in human atherosclerotic lesions...
November 2014: Kidney International
Michael Holzer, Markus Trieb, Viktoria Konya, Christian Wadsack, Akos Heinemann, Gunther Marsche
Most coronary deaths occur in patients older than 65years. Age associated alterations in the composition and function of high-density lipoproteins (HDL) may contribute to cardiovascular mortality. The effect of advanced age on the composition and function of HDL is not well understood. HDL was isolated from healthy young and elderly subjects. HDL composition, cellular cholesterol efflux/uptake, anti-oxidant properties and paraoxonase activity were assessed. We observed a 3-fold increase of the acute phase protein serum amyloid A, an increased content of complement C3 and proteins involved in endopeptidase/protease inhibition in HDL of elderly subjects, whereas levels of apolipoprotein E were significantly decreased...
September 2013: Biochimica et Biophysica Acta
Ivana Sreckovic, Ruth Birner-Gruenberger, Britta Obrist, Tatjana Stojakovic, Hubert Scharnagl, Michael Holzer, Monika Scholler, Sonia Philipose, Gunther Marsche, Uwe Lang, Gernot Desoye, Christian Wadsack
In human high-density lipoprotein (HDL) represents the major cholesterol carrying lipoprotein class in cord blood, while cholesterol is mainly carried by low-density lipoprotein in maternal serum. Additionally, to carrying cholesterol, HDL also associates with a range of proteins as cargo. We tested the hypothesis that fetal HDL carries proteins qualitatively and quantitatively different from maternal HDL. These differences then contribute to distinct HDL functionality in both circulations. Shotgun proteomics and biochemical analyses were used to assess composition/function of fetal and maternal HDL isolated from uncomplicated human pregnancies at term of gestation...
April 2013: Biochimica et Biophysica Acta
Mireille N M van Poppel, Willibald Zeck, Daniela Ulrich, Eva-Christina Schest, Birgit Hirschmugl, Uwe Lang, Christian Wadsack, Gernot Desoye
OBJECTIVE: Chemerin is a novel adipokine implicated in inflammation and obesity. We hypothesized that foetal chemerin would be elevated in gestational diabetes mellitus (GDM) and correlate with foetal and maternal adiposity. DESIGN: Observational, longitudinal study. SUBJECTS AND MEASUREMENTS: Foetal chemerin was measured separately in arterial and venous cord blood of 30 infants born to mothers with (n = 15) and without GDM (n = 15), in their mothers in early third trimester and at delivery and in amniotic fluid (week 32) of women with GDM...
January 2014: Clinical Endocrinology
Christian Wadsack, Gernot Desoye, Ursula Hiden
This review aims to provide a comprehensive summary of the aspects of endothelial and vascular dysfunction in the feto-placental vasculature occurring in pregnancy pathologies. This endothelium is continuous with the fetal circulation. Its function and potential dysfunction in pathologies will have a profound impact on fetal development. Gestational diabetes mellitus represents one of these pathologies, in which its associated metabolic derangements will alter feto-placental endothelial functions. These, in turn, may result in functional changes of the placenta, which may entail impaired fetal development...
May 2012: Wiener Medizinische Wochenschrift
Michael Holzer, Peter Wolf, Sanja Curcic, Ruth Birner-Gruenberger, Wolfgang Weger, Martin Inzinger, Dalia El-Gamal, Christian Wadsack, Akos Heinemann, Gunther Marsche
Psoriasis, a chronic inflammatory skin disease, has been linked to increased myocardial infarction and stroke. Functional impairment of HDL may contribute to the excess cardiovascular mortality of psoriatic patients. However, data available regarding the impact of psoriasis on HDL composition and function are limited. HDL from psoriasis patients and healthy controls was isolated by ultracentrifugation and shotgun proteomics, and biochemical methods were used to monitor changed HDL composition. We observed a significant reduction in apoA-I levels of HDL from psoriatic patients, whereas levels of apoA-II and proteins involved in acute-phase response, immune response, and endopeptidase/protease inhibition were increased...
August 2012: Journal of Lipid Research
Monika Scholler, Christian Wadsack, Jari Metso, Anil Paul Chirackal Manavalan, Ivana Sreckovic, Cornelia Schweinzer, Ursula Hiden, Matti Jauhiainen, Gernot Desoye, Ute Panzenboeck
CONTEXT: Phospholipid (PL) transfer protein (PLTP) plays a crucial role in high-density lipoprotein (HDL) metabolism. In the fetal circulation, HDL particles are the main cholesterol carriers and are involved in maternal-fetal cholesterol transfer across human placental endothelial cells (HPEC). OBJECTIVE: The aim was to investigate local function(s) of PLTP at the fetoplacental endothelium. Because HPEC display morphological and functional diversity when isolated from arteries or veins, we hypothesized that PLTP activity may differ between arterial and venous HPEC...
July 2012: Journal of Clinical Endocrinology and Metabolism
Monika Scholler, Christian Wadsack, Ingrid Lang, Karoline Etschmaier, Cornelia Schweinzer, Gunther Marsche, Martina Dieber-Rotheneder, Gernot Desoye, Ute Panzenboeck
CONTEXT: Gestational diabetes mellitus (GDM) causes alterations in fetal high-density lipoproteins (HDL). Because phospholipid transfer protein (PLTP) is important for HDL (re)assembly and is expressed in the human placenta, we hypothesized that circulating fetal and/or placental PLTP expression and activity are altered in GDM. DESIGN: PLTP levels and activity were determined in maternal and fetal sera from GDM and controls. Placental PLTP was immunolocalized, and its expression was measured in placental tissue...
February 2012: Journal of Clinical Endocrinology and Metabolism
Manuela Augsten, Hubert Hackl, Birgit Ebner, Angela Chemelli, Otto Glatter, Gunther Marsche, Uwe Lang, Gernot Desoye, Christian Wadsack
Maternal lipoproteins have been studied extensively in human pregnancies, but little is known about the role of fetal lipoproteins. The vascularized human placenta interfaces between the mother and fetus to transfer nutrients for sustaining pregnancy. Unlike that of adults, fetal high-density lipoprotein (HDL), which is in contact with placental vessels, is characterized by a high proportion of apolipoprotein E (apoE). We hypothesize this unique composition of fetal HDL affects key functions of the growing fetal tissues...
November 21, 2011: Physiological Genomics
Martin Gauster, Ursula Hiden, Mireille van Poppel, Sasa Frank, Christian Wadsack, Sylvie Hauguel-de Mouzon, Gernot Desoye
OBJECTIVE: This study addressed the hypothesis that placental endothelial lipase (EL) expression is affected by pregnancies complicated by obesity and gestational diabetes mellitus (GDM). RESEARCH DESIGN AND METHODS: EL expression in placental tissues from pregnancies complicated by obesity, GDM, or obesity combined with GDM (obese-GDM) was analyzed by quantitative RT-PCR. Moreover, primary placental cells were isolated and treated with insulin, glucose, leptin, or tumor necrosis factor (TNF)-α, and EL expression was measured...
October 2011: Diabetes
Dalia El-Gamal, Michael Holzer, Martin Gauster, Rudolf Schicho, Veronika Binder, Viktoria Konya, Christian Wadsack, Rufina Schuligoi, Akos Heinemann, Gunther Marsche
AIM: Recent work has shown that humans are significantly exposed to isocyanic acid/cyanate, which is generated when coal, biomass, or tobacco is burned. In vivo, cyanate is formed by the phagocyte protein myeloperoxidase and by breakdown of urea. Carbamylation of proteins through cyanate has been demonstrated to predict cardiovascular risk and is thought to promote vascular dysfunction; however, the underlying mechanisms remain unclear. RESULTS: Here, we show that cyanate induces intercellular cell adhesion molecule-1 (ICAM-1) expression with subsequently enhanced neutrophil adhesion in human coronary artery endothelial cells...
January 15, 2012: Antioxidants & Redox Signaling
Michael Holzer, Ruth Birner-Gruenberger, Tatjana Stojakovic, Dalia El-Gamal, Veronika Binder, Christian Wadsack, Akos Heinemann, Gunther Marsche
Functional impairment of HDL may contribute to the excess cardiovascular mortality experienced by patients with renal disease, but the effect of advanced renal disease on the composition and function of HDL is not well understood. Here, we used mass spectrometry and biochemical analyses to study alterations in the proteome and lipid composition of HDL isolated from patients on maintenance hemodialysis. We identified a significant increase in the amount of acute phase protein serum amyloid A1, albumin, lipoprotein-associated phospholipase A2, and apoC-III composing uremic HDL...
September 2011: Journal of the American Society of Nephrology: JASN
Emin Turkay Korgun, Gozde Unek, Emilio Herrera, Carolyn J Jones, Christian Wadsack, Dijle Kipmen-Korgun, Gernot Desoye
As cell cycle regulation is fundamental to the normal growth and development of the placenta, the aim of the present study was to determine the immunolocalizations of cell cycle related proteins, which have key roles in proliferation, differentiation and apoptosis during the development of the rat placenta. Here immunohistochemistry has been used to localize G1 cyclins (D1, D3, E), which are major determinants of proliferation, CIP/KIP inhibitors (p21, p27, p57), p53 as a master regulator and proliferating cell nuclear antigen in all cell types of the rat term placenta...
September 2011: Histochemistry and Cell Biology
Gernot Desoye, Martin Gauster, Christian Wadsack
The placenta is positioned between the maternal and fetal circulation and hence plays a key role in transporting maternal nutrients to the developing fetus. Fetal growth changes in the 2 most frequent pregnancy pathologies, gestational diabetes mellitus and fetal growth restriction, are predominantly characterized by an exaggerated and restricted fat accretion, respectively. Glucose, by its regulating effect on fetal insulin concentrations, and lipids have been strongly implicated in fetal fat deposition. Transplacental glucose flux is highly efficient and limited only by nutrient availability (flow-limited)--ie, driven by the maternal-fetal glucose concentration gradient and blood flow, with little, if any, effect of placental morphology, glucose consumption, and transporter expression...
December 2011: American Journal of Clinical Nutrition
Michael Holzer, Martin Gauster, Thomas Pfeifer, Christian Wadsack, Guenter Fauler, Philipp Stiegler, Harald Koefeler, Eckhard Beubler, Rufina Schuligoi, Akos Heinemann, Gunther Marsche
Carbamylation of proteins through reactive cyanate has been demonstrated to predict an increased cardiovascular risk. Cyanate is formed in vivo by breakdown of urea and at sites of inflammation by the phagocyte protein myeloperoxidase. Because myeloperoxidase (MPO) associates with high-density lipoprotein (HDL) in human atherosclerotic intima, we examined in the present study whether cyanate specifically targets HDL. Mass spectrometry analysis revealed that protein carbamylation is a major posttranslational modification of HDL...
June 15, 2011: Antioxidants & Redox Signaling
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