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16S sepsis

Rikke Heidemann Olsen, Henrik Christensen, Susanne Kabell, Magne Bisgaard
Pododermatitis has been observed in several layer flocks in Denmark during 2015. The aetiology is complex including litter quality, nutrition, management etc. Bacterial pathogens associated with pododermatitis, however, have not received much attention. The aim of the present study was therefore to identify 106 bacterial isolates obtained from pododermatitis in table egg layers in addition to five isolates from spleen/bursa presternalis. Isolates were obtained from layers from six affected flocks. All isolates were identified by standard bacterial methods, species-specific PCRs, 16S rRNA sequencing or Matrix-assisted laser desorption-ionization identification...
March 8, 2018: Avian Pathology: Journal of the W.V.P.A
Binoy Mathews Nedumgottil
Aims and Objective: Oral biofilms in denture wearers are populated with a large number of bacteria, a few of which have been associated with medical conditions such as sepsis and infective endocarditis (IE). The present study was designed to investigate the relative presence of pathogenic bacteria in biofilms of denture wearers specifically those that are associated with IE. Methods: Biofilm samples from 88 denture wearers were collected and processed to extract total genomic DNA...
January 2018: Journal of Indian Prosthodontic Society
Romy D Zwittink, Ingrid B Renes, Richard A van Lingen, Diny van Zoeren-Grobben, Prokopis Konstanti, Obbe F Norbruis, Rocio Martin, Liesbeth J M Groot Jebbink, Jan Knol, Clara Belzer
Antibiotic treatment is common practice in the neonatal ward for the prevention and treatment of sepsis, which is one of the leading causes of mortality and morbidity in preterm infants. Although the effect of antibiotic treatment on microbiota development is well recognised, little attention has been paid to treatment duration. We studied the effect of short and long intravenous antibiotic administration on intestinal microbiota development in preterm infants. Faecal samples from 15 preterm infants (35 ± 1 weeks gestation and 2871 ± 260 g birth weight) exposed to no, short (≤ 3 days) or long (≥ 5 days) treatment with amoxicillin/ceftazidime were collected during the first six postnatal weeks...
January 24, 2018: European Journal of Clinical Microbiology & Infectious Diseases
Richard G Brown, Julian R Marchesi, Yun S Lee, Ann Smith, Benjamin Lehne, Lindsay M Kindinger, Vasso Terzidou, Elaine Holmes, Jeremy K Nicholson, Phillip R Bennett, David A MacIntyre
BACKGROUND: Preterm prelabour rupture of the fetal membranes (PPROM) precedes 30% of preterm births and is a risk factor for early onset neonatal sepsis. As PPROM is strongly associated with ascending vaginal infection, prophylactic antibiotics are widely used. The evolution of vaginal microbiota compositions associated with PPROM and the impact of antibiotics on bacterial compositions are unknown. METHODS: We prospectively assessed vaginal microbiota prior to and following PPROM using MiSeq-based sequencing of 16S rRNA gene amplicons and examined the impact of erythromycin prophylaxis on bacterial load and community structures...
January 24, 2018: BMC Medicine
Benjamin H Singer, Robert P Dickson, Scott J Denstaedt, Michael W Newstead, Kwi Kim, Nicole R Falkowski, John R Erb-Downward, Thomas M Schmidt, Gary B Huffnagle, Theodore J Standiford
RATIONALE: Sepsis causes brain dysfunction and neuroinflammation. It is unknown whether neuroinflammation in sepsis is initiated by dissemination of bacteria to the brain and sustained by persistent infection, or whether neuroinflammation is a sterile process resulting solely from circulating inflammatory mediators. OBJECTIVES: To determine if gut bacteria translocate to the brain during sepsis, and are associated with neuroinflammation. METHODS: Murine sepsis was induced using cecal ligation and puncture, and sepsis survivor mice were compared with sham and unoperated control animals...
March 15, 2018: American Journal of Respiratory and Critical Care Medicine
Marcelo Pillonetto, Lavinia N Arend, Helisson Faoro, Helena R S D'Espindula, Jochen Blom, Theo H M Smits, Marcelo T Mira, Fabio Rezzonico
The species Phytobacter diazotrophicus and the associated genus Phytobacter were originally described by Zhanget al. [Arch Microbiol189 (2008), 431-439] on the basis of few endophytic nitrogen-fixing bacteria isolated from wild rice (Oryza rufipogon) in China. In this study, we demonstrate that a number of clinical isolates that were either described in the literature, preserved in culture collections, or obtained during a 2013 multi-state sepsis outbreak in Brazil also belong to the same genus. 16S rRNA gene sequencing, multilocus sequence analysis based on gyrB, rpoB, atpD and infB genes, as well as digital DNA-DNA hybridization support the existence of a second species within the genus Phytobacter...
November 10, 2017: International Journal of Systematic and Evolutionary Microbiology
Isela García-Gudiño, Eucario Yllescas-Medrano, Rolando Maida-Claros, Diana Soriano-Becerril, Nestor F Díaz, Guadalupe García-López, Anayansí Molina-Hernández, Oscar Flores-Herrera, Francisco J Zavala-Díaz de la Serna, María Del Rosario Peralta-Pérez, Héctor Flores-Herrera
It is estimated that 15% of all newborns admitted to the neonatal intensive care unit (NICU) for suspected sepsis receive multiple broad-spectrum antibiotics without pathogen identification. The gold standard for bacterial sepsis detection is blood culture, but the sensitivity of this method is very low. Recently, amplification and analysis of the 16S ribosomal DNA (rDNA) bacterial gene in combination with denaturing gradient gel electrophoresis (DGGE) has proven to be a useful approach for identifying bacteria that are difficult to isolate by standard culture methods...
October 31, 2017: European Journal of Pediatrics
Suyan Li, Jian Lv, Jianguo Li, Zhaolong Zhao, Hui Guo, Yanni Zhang, Shichao Cheng, Jianbin Sun, Hongming Pan, Shaopeng Fan, Zhongxin Li
OBJECTIVE: The pathogenesis of sepsis associated encephalopathy (SAE) remains poorly understood. Vagus nerve plays an important role in gut-microbiota-brain axis. This study aimed to investigate whether vague nerve is a key mediator of the impact of intestinal microbiota on SAE. METHODS: Male rats were randomly divided into four groups (n=20): SHAM (SH) group, lipopolysaccharide (LPS) group, fecal microbiota transplantation (FMT) +LPS group, and vagotomy (VGX)+LPS+FMT group...
October 6, 2017: Neuroscience Letters
Irshad M Sulaiman, Pratik Banerjee, Ying-Hsin Hsieh, Nancy Miranda, Steven Simpson, Khalil Kerdahi
Staphylococcus spp. is considered as one of the most common human-pathogenic bacteria, causing illnesses ranging from nonthreatening skin infections to lethal diseases, including sepsis, pneumonia, bloodstream infections, and food poisoning. The emergence of methicillin-resistant Staphylococcus aureus strains has increased morbidity and mortality and resulted in a major healthcare burden worldwide. Single and multilocus sequence typing have been extensively used in the identification of Staphylococcus species...
September 15, 2017: Journal of AOAC International
Andrea Ticinesi, Christian Milani, Fulvio Lauretani, Antonio Nouvenne, Leonardo Mancabelli, Gabriele Andrea Lugli, Francesca Turroni, Sabrina Duranti, Marta Mangifesta, Alice Viappiani, Chiara Ferrario, Marcello Maggio, Marco Ventura, Tiziana Meschi
Reduced biodiversity and increased representation of opportunistic pathogens are typical features of gut microbiota composition in aging. Few studies have investigated their correlation with polypharmacy, multimorbidity and frailty. To assess it, we analyzed the fecal microbiota from 76 inpatients, aged 83 ± 8. Microbiome biodiversity (Chao1 index) and relative abundance of individual bacterial taxa were determined by next-generation 16S rRNA microbial profiling. Their correlation with number of drugs, and indexes of multimorbidity and frailty were verified using multivariate linear regression models...
September 11, 2017: Scientific Reports
Sebastian M Toescu, Sandra Lacey, Hu Liang Low
A 49-year-old female underwent multiple craniotomies for resection of recurrent malignant meningiomata (WHO grade III). She re-presented with sepsis due to a ventricular empyema. The craniotomy wound was urgently debrided, and isolates of the gram-negative rod, Weeksella virosa, were identified on 16S PCR. This species is most commonly found as a genitourinary commensal. We present the first documented intracranial infection by Weeksella virosa and its successful treatment with oral β-lactam antibiotics.
November 2017: Acta Neurochirurgica
Iva Kotaskova, Petr Nemec, Martina Vanerkova, Barbora Malisova, Renata Tejkalova, Marek Orban, Vita Zampachova, Tomas Freiberger
BACKGROUND: The presence of more than one bacterial agent is relatively rare in infective endocarditis, although more common in prosthetic cases. Molecular diagnosis from a removed heart tissue is considered a quick and effective way to diagnose fastidious or intracellular agents. CASE PRESENTATION: Here we describe the case of postpartum polymicrobial prosthetic valve endocarditis in a young woman. Sneathia sanguinegens and Mycoplasma hominis were simultaneously detected from the heart valve sample using broad range 16S rRNA polymerase chain reaction (PCR) followed by sequencing while culture remained negative...
August 14, 2017: BMC Infectious Diseases
Gregory R Young, Darren L Smith, Nicholas D Embleton, Janet E Berrington, Edward C Schwalbe, Stephen P Cummings, Christopher J van der Gast, Clare Lanyon
Necrotising enterocolitis (NEC) and sepsis are serious diseases of preterm infants that can result in feeding intolerance, the need for bowel resection, impaired physiological and neurological development, and high mortality rates. Neonatal healthcare improvements have allowed greater survival rates in preterm infants leading to increased numbers at risk of developing NEC and sepsis. Gut bacteria play a role in protection from or propensity to these conditions and have therefore, been studied extensively using targeted 16S rRNA gene sequencing methods...
2017: Frontiers in Cellular and Infection Microbiology
Jieun Kim, Sung Kuk Hong, Myungsook Kim, Dongeun Yong, Kyungwon Lee
Urosepsis due to Aerococcus urinae is rare in clinical settings with only a few of reported cases worldwide by 16S rRNA sequencing. Here we report a case of sepsis caused by A. urinae in a 86 year-old male with complicated urinary tract infection which was confirmed through peptide mass fingerprinting of matrix-assisted laser desorption ionization time of flight mass spectrometry.
September 2017: Infection & Chemotherapy
K H W Dautzenberg, F N Polderman, R J van Suylen, M A M Moviat
Both purpura fulminans and toxic epidermal necrolysis (TEN) are rare and life-threatening disorders with a high mortality. We present a case of suspected rapidly progressive, severe pneumococcal sepsis-induced purpura fulminans complicated by multiple organ failure, severe epidermolysis and cutaneous necrosis. We show the diagnostic challenge to differentiate between purpura fulminans and TEN, as the extensive epidermolysis in purpura fulminans may mimic TEN and we highlight the additional value of repeated skin biopsies and 16S rRNA gene sequencing...
May 2017: Netherlands Journal of Medicine
Chang-Feng Liu, Xin-Ping Shi, Yun Chen, Ye Jin, Bing Zhang
BACKGROUND: The survival rate of septic patients mainly depends on a rapid and reliable diagnosis. A rapid, broad range, specific and sensitive quantitative diagnostic test is the urgent need. Thus, we developed a TaqMan-Based Multiplex real-time PCR assays to identify bloodstream pathogens within a few hours. METHODS: Primers and TaqMan probes were designed to be complementary to conserved regions in the 16S rDNA gene of different kinds of bacteria. To evaluate accurately, sensitively, and specifically, the known bacteria samples (Standard strains, whole blood samples) are determined by TaqMan-Based Multiplex real-time PCR...
May 17, 2017: Journal of Clinical Laboratory Analysis
Anne-Marie Rick, Angie Aguilar, Rosita Cortes, Remei Gordillo, Mario Melgar, Gabriela Samayoa-Reyes, Daniel N Frank, Edwin J Asturias
BACKGROUND: Infection causes 1 of every 5 neonatal deaths globally. Group B Streptococcus (GBS) is the most significant pathogen, although little is known about its epidemiology and risk in low-income countries. METHODS: A cross-sectional study in 2015 at a public hospital in Guatemala City enrolled women ≥35 weeks' gestation. Vaginal and rectal swabs were processed using Lim broth and GBS CHROMagar then agglutination testing. Risk factors were assessed using multivariate analysis...
2017: Open Forum Infectious Diseases
Samantha J Thulborn, Madiha Dilpazir, Koirobi Haldar, Vijay Mistry, Christopher E Brightling, Michael R Barer, Mona Bafadhel
BACKGROUND: Pentraxin 3 (PTX3) is an acute phase protein, involved in antibacterial resistance. Recent studies have shown PTX3 levels to be elevated in the presence of a bacterial infection and in a murine sepsis model. OBJECTIVE: We aim to investigate if sputum PTX3 can be used as a biomarker for bacterial infection in subjects with COPD. MATERIALS AND METHODS: Sputum samples from 142 COPD patients (102 men) with a mean (range) age of 69 years (45-85) and mean (SD) post-bronchodilator percentage predicted forced expiratory volume in 1 second (FEV1) of 50% (19) were analyzed for PTX3, using a commercial assay at stable state and during an exacerbation...
2017: International Journal of Chronic Obstructive Pulmonary Disease
Jacqueline M Lankelma, Emma Birnie, Tassili A F Weehuizen, Brendon P Scicluna, Clara Belzer, Riekelt H Houtkooper, Joris J T H Roelofs, Alex F de Vos, Tom van der Poll, Andries E Budding, W Joost Wiersinga
BACKGROUND: Melioidosis, caused by the Gram-negative bacterium Burkholderia pseudomallei, is an emerging cause of pneumonia-derived sepsis in the tropics. The gut microbiota supports local mucosal immunity and is increasingly recognized as a protective mediator in host defenses against systemic infection. Here, we aimed to characterize the composition and function of the intestinal microbiota during experimental melioidosis. METHODOLOGY/PRINCIPAL FINDINGS: C57BL/6 mice were infected intranasally with B...
April 2017: PLoS Neglected Tropical Diseases
Harish Punia, Geeta Gathwala, Dhara B Dhaulakhandi, Mohammed Aamir
The gold standard for detecting bacterial sepsis is blood culture. However, the sensitivity of blood culture is low and the results take 48-72 h. Molecular assays for the detection of bacterial DNA permit early detection of a bacterial cause as the turnaround time is 6-8 h. We undertook an evaluation of the performance of universal bacterial primer (16S rRNA) polymerase chain reaction (PCR) in the diagnosis of neonatal sepsis at a tertiary care medical college teaching hospital. 16S rRNA PCR was positive in all cases of blood culture proven sepsis...
January 1, 2017: Tropical Doctor
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