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16S sepsis

Yanling Wei, Jun Yang, Jun Wang, Yang Yang, Juan Huang, Hao Gong, Hongli Cui, Dongfeng Chen
BACKGROUND: The dysbiosis of intestinal microbiota plays an important role in the development of gut-derived infections, making it a potential therapeutic target against multiple organ dysfunction syndrome (MODS) after sepsis. However, the effectiveness of fecal microbiota transplantation (FMT) in treating this disease has been rarely investigated. METHODS: Two male patients, a 65-year-old and an 84-year-old, were initially diagnosed with cerebellar hemorrhage and cerebral infarction, respectively, after admission...
October 18, 2016: Critical Care: the Official Journal of the Critical Care Forum
Piyush Jha, Choon-Mee Kim, Dong-Min Kim, Jong-Hoon Chung, Na-Ra Yoon, Babita Jha, Seok Won Kim, Sook Jin Jang, Young-Joon Ahn, Jae Keun Chung, Doo Young Jeon
Enterobacter aerogenes is recognized as an important bacterial pathogen in hospital-acquired infections. This report describes two unusual cases of septicemia caused by E. aerogenes in immunocompetent healthcare workers. E. aerogenes was isolated from blood cultures of the two patients experiencing septicemia. The clinical isolates were initially identified as E. aerogenes using a VITEK II automated system and 16S rRNA sequence analysis, and; both isolates involved in the outbreak shared a common pulse-field gel electrophoresis pattern...
2016: SpringerPlus
Chandirasegaran Massilamany, Akram Mohammed, John Dustin Loy, Tanya Purvis, Bharathi Krishnan, Rakesh H Basavalingappa, Christy M Kelley, Chittibabu Guda, Raúl G Barletta, Etsuko N Moriyama, Timothy P L Smith, Jay Reddy
BACKGROUND: We recently reported the identification of Bacillus sp. NRRL B-14911 that induces heart autoimmunity by generating cardiac-reactive T cells through molecular mimicry. This marine bacterium was originally isolated from the Gulf of Mexico, but no associations with human diseases were reported. Therefore, to characterize its biological and medical significance, we sought to determine and analyze the complete genome sequence of Bacillus sp. NRRL B-14911. RESULTS: Based on the phylogenetic analysis of 16S ribosomal RNA (rRNA) genes, sequence analysis of the 16S-23S rDNA intergenic transcribed spacers, phenotypic microarray, and matrix-assisted laser desorption ionization time-of-flight mass spectrometry, we propose that this organism belongs to the species Bacillus infantis, previously shown to be associated with sepsis in a newborn child...
2016: BMC Genomics
Barış Ata Borsa, Mehmet Ersoy Aldağ, Birsen Tunalı, Uğur Dinç, Zeynep Güngördü Dalar, Veli Cengiz Özalp
The high prevalence of Bacillus species in nature and the detection of these bacteria as contaminant in cultures may lead diagnostic dilemma, however they should still be considered as a pathogen particularly in case of repeated positive cultures from patients with risk factors. Bacillus pumilus is a bacteria, though rarely, been reported as the causative agent of various infections such as sepsis, endocarditis, skin infections and food poisoning in human. In this report, a sepsis case in an immunocompetent patient caused by B...
July 2016: Mikrobiyoloji Bülteni
Yakov Vitrenko, Iryna Kostenko, Kateryna Kulebyakina, Alla Duda, Mariya Klunnyk, Khrystyna Sorochynska
Cell preparations to be used in clinical practice must be free of infectious agents. Safety concerns are especially elevated upon the use of human fetal tissues which are otherwise highly advantageous in cell therapy. We demonstrate that treating fetal samples with antibiotic, extensive washing and homogenization prior to cryoconservation efficiently removes microbes in general. Screening a large collection by an automatic culture system showed that 89% fetal tissue samples were sterile while contamination was detected in 10...
August 5, 2016: Cell Transplantation
Celeste M Lavallee, Jayden A R MacPherson, Mi Zhou, Yanhua Gao, Pamela R Wizzard, Paul W Wales, Justine M Turner, Benjamin P Willing
BACKGROUND: Total parenteral nutrition (TPN) is a cause of intestinal microbial dysbiosis and impaired gut barrier function. This may contribute to life-threatening parenteral nutrition-associated liver disease and sepsis in infants. We compared the effects of a lipid emulsion containing long-chain ω-3 polyunsaturated fatty acids (PUFAs; SMOFlipid) and a predominantly ω-6 PUFA emulsion (Intralipid) on microbial composition and host response at the mucosal surface. MATERIALS AND METHODS: Neonatal piglets were provided isocaloric, isonitrogenous TPN for 14 days versus sow-fed (SF) controls...
August 5, 2016: JPEN. Journal of Parenteral and Enteral Nutrition
Dina A Midan, Wafaa Moustafa M Abo El Fotoh, Abeer H El Shalakany
OBJECTIVES: This study aimed to explore whether 16S rRNA gene amplification by real time PCR and sequencing could serve as genetic based methods in rapid and accurate diagnosis of neonatal sepsis. PATIENTS AND METHODS: This case control study was conducted on 40 neonates suffering from sepsis like manifestations recruited from the neonatal intensive care unit of Menoufia university hospital over a period of 6 months. Their blood samples were used for paired analysis of bacterial growth using BACTEC 9050 instrument and real time PCR assay with subsequent DNA sequencing for bacterial species identification...
August 2, 2016: Journal of Maternal-fetal & Neonatal Medicine
Ana Luisa Kalb Lopes, Josiane Cardoso, Fernanda Roberta Correa Cleto Dos Santos, Ana Claudia Graziani Silva, Maria Isabel Stets, Nilson Ivo Tonin Zanchin, Maurilio José Soares, Marco Aurélio Krieger
Bloodstream infections are important public health problems, associated with high mortality due to the inability to detect the pathogen quickly in the early stages of infection. Such inability has led to a growing interest in the development of a rapid, sensitive, and specific assay to detect these pathogens. In an effort to improve diagnostic efficiency, we present here a magnetic separation method for bacteria that is based on mutated lysozyme (LysE35A) to capture S. aureus from whole blood. LysE35A-coated beads were able to bind different MSSA and MRSA isolates in the blood and also other six Gram-positive and two Gram-negative species in whole blood...
September 2016: Journal of Microbiological Methods
Lorrance L Majewski, Masayuki Nogi, Matthew J Bankowski, Heath H Chung
Bordetella is a gram-negative, glucose non-fermenting bacillus, consisting of many host-associated species. B. trematum has previously been identified in wound infections, but rarely known to be a source of bacteremia. Currently, 16S rRNA sequencing represents the reference standard method by which identification is made. Herein, we present a case of fatal B. trematum bacteremia with septic shock. The presumed primary site of the infection was a rapidly developing left leg deep soft tissue infection without necrotizing fasciitis...
September 2016: Diagnostic Microbiology and Infectious Disease
Steven J Schiff, Julius Kiwanuka, Gina Riggio, Lan Nguyen, Kevin Mu, Emily Sproul, Joel Bazira, Juliet Mwanga-Amumpaire, Dickson Tumusiime, Eunice Nyesigire, Nkangi Lwanga, Kaleb T Bogale, Vivek Kapur, James R Broach, Sarah U Morton, Benjamin C Warf, Mary Poss
Neonatal sepsis (NS) is responsible for over 1 million yearly deaths worldwide. In the developing world, NS is often treated without an identified microbial pathogen. Amplicon sequencing of the bacterial 16S rRNA gene can be used to identify organisms that are difficult to detect by routine microbiological methods. However, contaminating bacteria are ubiquitous in both hospital settings and research reagents and must be accounted for to make effective use of these data. In this study, we sequenced the bacterial 16S rRNA gene obtained from blood and cerebrospinal fluid (CSF) of 80 neonates presenting with NS to the Mbarara Regional Hospital in Uganda...
2016: Frontiers in Medicine
A E Nieman, P H M Savelkoul, A Beishuizen, B Henrich, B Lamik, C R MacKenzie, D Kindgen-Milles, A Helmers, C Diaz, S G Sakka, R P Schade
BACKGROUND: Rapid diagnosis and appropriate antimicrobial therapy are of major importance to decrease morbidity and mortality in patients with blood stream infections (BSI). Blood culture, the current gold standard for detecting bacteria in blood, requires at least 24-48 hours and has limited sensitivity if obtained during antibiotic treatment of the patient. The aim of this prospective multicenter study was to clinically evaluate the application of a commercial universal 16S/18S rDNA PCR, SepsiTest™ (PCR-ST), directly on whole blood...
2016: BMC Infectious Diseases
Alejandro Carabarin-Lima, Libia León-Izurieta, Rosa Del Carmen Rocha-Gracia, Miguel Castañeda-Lucio, Carmen Torres, Zita Gutiérrez-Cazarez, Sirenia González-Posos, Claudia F Martínez de la Peña, Ygnacio Martinez-Laguna, Patricia Lozano-Zarain
The genus Klebsiella belongs to the family Enterobacteriaceae, and is currently considered to be non-motile and non-flagellated. In the present work, 25 Klebsiella strains isolated from nosocomial infections were assessed for motility under different growth conditions. One Klebsiella isolate, KpBUAP021, demonstrated a swim-like motility phenotype. The K. pneumoniae genotype was confirmed by 16S rRNA and rpoB gene sequence analysis. Multilocus sequence typing analysis also revealed that the KpBUAP021 strain places it in the ST345 sequence type, and belongs to the phylogenetic Kpl group...
August 2016: Journal of Medical Microbiology
Ngo Tat Trung, Tran Thi Thu Hien, Tran Thi Thanh Huyen, Dao Thanh Quyen, Trinh Van Son, Phan Quoc Hoan, Nguyen Thi Kim Phuong, Tran Thi Lien, Mai Thanh Binh, Hoang Van Tong, Christian G Meyer, Thirumalaisamy P Velavan, Le Huu Song
BACKGROUND: Blood cultures are commonly employed to identify bacterial pathogens causing sepsis. PCR assays to diagnose septicemia require extraction of bacterial DNA from blood samples and thus, delay the initiation of appropriate antimicrobial treatment. The presence of abundant human DNA may hamper the sensitivity of PCR in the detection of bacteria. METHODS: We used serial dilutions of E. Coli spiked pseudo-blood-sepsis samples to develop a simple method that combines the use of a polar detergent solvent and adjustment of the basic pH to remove human DNA...
2016: BMC Infectious Diseases
K W Pang, L Coorevits, J Van Acker, W Pauwels
Enterococcus cecorum septicemia is rare in humans. This case describes a man with underlying liver cirrhosis, a comorbidity that contributes to half of the E. cecorum infections described in the current literature. The patient was successfully treated with ceftriaxone. Identification of this species was accurately made with Matrix-Assisted Laser Desorption-Ionization Time-of-Flight Mass Spectrometry and confirmed by 16S rDNA sequencing.
April 26, 2016: Acta Clinica Belgica
Mahua Das Gupta, Harsimran Kaur, Pallab Ray, Vikas Gautam, G D Puri
Early diagnosis and treatment of sepsis by appropriate antibiotics is of utmost importance. Therefore, we evaluated 16S rRNA panbacterial polymerase chain reaction (PCR) for rapid diagnosis of sepsis in 49 adult patients in Intensive Care Units (ICUs) and compared it with an automated blood culture. 8 ml of 10 ml blood collected was inoculated into BACTEC® aerobic bottle and the remaining 2 ml was used for DNA extraction and PCR. 109 of 115 (93%) episodes of suspected sepsis showed concordant results between automated culture and PCR...
April 2016: Indian Journal of Medical Microbiology
Anusha Rohit, Biswajit Maiti, Shalini Shenoy, Indrani Karunasagar
BACKGROUND & OBJECTIVES: The difficulties in diagnosis of neonatal sepsis are due to varied clinical presentation, low sensitivity of blood culture which is considered the gold standard and empirical antibiotic usage affecting the outcome of results. Though polymerase chain reaction (PCR) based detection of bacterial 16S rRNA gene has been reported earlier, this does not provide identification of the causative agent. In this study, we used restriction fragment length polymorphism (RFLP) of amplified 16S rRNA gene to identify the organisms involved in neonatal sepsis and compared the findings with blood culture...
January 2016: Indian Journal of Medical Research
Salah Zangenah, Nasir Abbasi, Anders F Andersson, Peter Bergman
C. canimorsus and C. cynodegmi are dog and cat commensals which can be transmitted to humans via bites or scratches and can cause sepsis, meningitis, endocarditis, and eye- or wound infections. Recently an additional Capnocytophaga species was identified as part of the oral flora of healthy dogs and was given the name "C. canis". We previously identified a Capnocytophaga isolate that could not be typed with available diagnostic tests including MALDI-TOF, 16S rRNA sequencing or species-specific PCR. This strain and 21 other Capnocytophaga spp isolated in Sweden from clinical blood- or wound-cultures were subjected to whole genome sequencing using the Illumina platform...
2016: Scientific Reports
Min Young Lee, Myeong Hee Kim, Woo In Lee, So Young Kang, You La Jeon
Aerococcus urinae is an uncommon pathogen that was first identified in 1992. Herein, we report a case of bloodstream infection caused by A. urinae, which occurred in a 92-year-old Korean female patient with an underlying urologic infection who had altered consciousness. The blood culture yielded positive results for A. urinae; however, identifying A. urinae was challenging. Ultimately, we used 16S ribosomal RNA (rRNA) gene sequencing to identify the organism. The patient recovered after being treated with ertapenem and meropenem...
May 2016: Laboratory Medicine
A Torri, F Delbianco, F D Baccarini, M Fusari, S Bertini, F Congestrì, M Matteucci, M F Pedna, G D Testa, V Sambri
The first isolation of Catabacter hongkongensis in Italy is reported. Pleomorphic Gram-positive rods were grown in blood cultures from samples obtained from a 55-year-old patient admitted to the intensive care unit with sepsis after he experienced massive thoracic and abdominal trauma. The identification was obtained by amplification and sequencing of the 16S rRNA gene.
January 2016: New Microbes and New Infections
Anne Krüger, Marina Oldenburg, Chiranjeevi Chebrolu, Daniela Beisser, Julia Kolter, Anna M Sigmund, Jörg Steinmann, Simon Schäfer, Hubertus Hochrein, Sven Rahmann, Hermann Wagner, Philipp Henneke, Veit Hornung, Jan Buer, Carsten J Kirschning
Toll-like receptor (TLR) 13 and TLR2 are the major sensors of Gram-positive bacteria in mice. TLR13 recognizes Sa19, a specific 23S ribosomal (r) RNA-derived fragment and bacterial modification of Sa19 ablates binding to TLR13, and to antibiotics such as erythromycin. Similarly, RNase A-treated Staphylococcus aureus activate human peripheral blood mononuclear cells (PBMCs) only via TLR2, implying single-stranded (ss) RNA as major stimulant. Here, we identify human TLR8 as functional TLR13 equivalent that promiscuously senses ssRNA...
December 2015: EMBO Reports
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