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https://www.readbyqxmd.com/read/27836711/effect-of-cigarette-smoke-extract-on-p-glycoprotein-function-in-primary-cultured-and-newly-developed-alveolar-epithelial-cells
#1
Mikihisa Takano, Ryosuke Naka, Yoshihiro Sasaki, Saori Nishimoto, Ryoko Yumoto
The effect of cigarette smoke extract (CSE) on P-glycoprotein (P-gp) function in the distal lung is unclear. In this study, we first examined the expression and function of P-gp and the effect of CSE in rat primary cultured alveolar epithelial cells. The expression of P-gp protein was observed in type I-like cells, but not in type II cells. In type I-like cells, rhodamine 123 (Rho123) accumulation was enhanced by various P-gp inhibitors such as verapamil and cyclosporine A. In addition, the expression of P-gp mRNAs, mdr1a and mdr1b, as well as P-gp activity increased along with the transdifferentiation...
August 31, 2016: Drug Metabolism and Pharmacokinetics
https://www.readbyqxmd.com/read/27601334/polysorbate-20-increases-oral-absorption-of-digoxin-in-wild-type-sprague-dawley-rats-but-not-in-mdr1a-sprague-dawley-rats
#2
Carsten Uhd Nielsen, Ahmed A Abdulhussein, Dilan Colak, René Holm
The aim was to investigate the ability of polysorbate 20 to alter oral digoxin absorption in vitro and drug exposure in vivo via modulation of transporter mediated efflux. Transport studies were performed in MDCKII-MDR1 and Caco-2 cells using (3)H-digoxin. Pharmacokinetic studies were performed in wild type and mdr1a deficient Sprague Dawley rats. (3)H-digoxin was quantified using liquid scintillation counting. The results showed an increased absorptive transport and a reduced secretory transport in MDCKII-MDR and Caco-2 cells as a function of polysorbate 20 concentrations...
September 4, 2016: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/27555820/inhibition-of-p-glycoprotein-and-multidrug-resistance-associated-protein-2-regulates-the-hepatobiliary-excretion-and-plasma-exposure-of-thienorphine-and-its-glucuronide-conjugate
#3
Ling-Lei Kong, Guo-Lin Shen, Zhi-Yuan Wang, Xiao-Mei Zhuang, Wei-Bin Xiao, Mei Yuan, Ze-Hui Gong, Hua Li
Thienorphine (TNP) is a novel partial opioid agonist that has completed phase II clinical evaluation as a promising drug candidate for the treatment of opioid dependence. Previous studies have shown that TNP and its glucuronide conjugate (TNP-G) undergo significant bile excretion. The purpose of this study was to investigate the roles of efflux transporters in regulating biliary excretion and plasma exposure of TNP and TNP-G. An ATPase assay suggested that TNP and TNP-G were substrates of P-gp and MRP2, respectively...
2016: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/27276518/quantitative-targeted-absolute-proteomics-of-transporters-and-pharmacoproteomics-based-reconstruction-of-p-glycoprotein-function-in-mouse-small-intestine
#4
Takanori Akazawa, Yasuo Uchida, Masanori Tachikawa, Sumio Ohtsuki, Tetsuya Terasaki
The purpose of this study was to investigate whether a pharmacokinetic model integrating in vitro mdr1a efflux activity (which we previously reported) with in vitro/in vivo differences in protein expression level can reconstruct intestinal mdr1a function. In situ intestinal permeability-surface area product ratio between wild-type and mdr1a/1b (-/-) mice is one of the parameters used to describe intestinal mdr1a function. The reconstructed ratios of six mdr1a substrates (dexamethasone, digoxin, loperamide, quinidine, verapamil, vinblastine) and one nonsubstrate (diazepam) were consistent with the observed values reported by Adachi et al...
July 5, 2016: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/27139220/oral-serum-derived-bovine-immunoglobulin-protein-isolate-has-immunomodulatory-effects-on-the-colon-of-mice-that-spontaneously-develop-colitis
#5
Anna Pérez-Bosque, Lluïsa Miró, Mònica Maijó, Javier Polo, Joy M Campbell, Louis Russell, Joe D Crenshaw, Eric Weaver, Miquel Moretó
Dietary immunoglobulin concentrates prepared from animal plasma can modulate the immune response of gut-associated lymphoid tissue (GALT). Previous studies have revealed that supplementation with serum-derived bovine immunoglobulin/protein isolate (SBI) ameliorates colonic barrier alterations in the mdr1a-/- genetic mouse model of IBD. Here, we examine the effects of SBI on mucosal inflammation in mdr1a-/- mice that spontaneously develop colitis. Wild type (WT) mice and mice lacking the mdr1a gene (KO) were fed diets supplemented with either SBI (2% w/w) or milk proteins (Control diet), from day 21 (weaning) until day 56...
2016: PloS One
https://www.readbyqxmd.com/read/27026287/visualizing-spatial-distribution-of-alectinib-in-murine-brain-using-quantitative-mass-spectrometry-imaging
#6
Hiroaki Aikawa, Mitsuhiro Hayashi, Shoraku Ryu, Makiko Yamashita, Naoto Ohtsuka, Masanobu Nishidate, Yasuhiro Fujiwara, Akinobu Hamada
In the development of anticancer drugs, drug concentration measurements in the target tissue have been thought to be crucial for predicting drug efficacy and safety. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is commonly used for determination of average drug concentrations; however, complete loss of spatial information in the target tissue occurs. Mass spectrometry imaging (MSI) has been recently applied as an innovative tool for detection of molecular distribution of pharmacological agents in heterogeneous targets...
2016: Scientific Reports
https://www.readbyqxmd.com/read/26995548/methods-of-inducing-inflammatory-bowel-disease-in-mice
#7
Byoungwook Bang, Lenard M Lichtenberger
Animal models of experimentally induced inflammatory bowel disease (IBD) are useful for understanding more about the mechanistic basis of the disease, identifying new targets for therapeutic intervention, and testing novel therapeutics. This unit provides detailed protocols for five widely used mouse models of experimentally induced intestinal inflammation: chemical induction of colitis by dextran sodium sulfate (DSS), hapten-induced colitis via 2,4,6-trinitrobenzene sulfonic acid (TNBS), Helicobacter-induced colitis in mdr1a(-/-) mice, the CD4(+) CD45RB(hi) SCID transfer colitis model, and the IL-10(-/-) colitis model...
2016: Current Protocols in Pharmacology
https://www.readbyqxmd.com/read/26971931/a-role-for-p-glycoprotein-in-clearance-of-alzheimer-amyloid-%C3%AE-peptide-from-the-brain
#8
Wei Wang, Angela M Bodles-Brakhop, Steven W Barger
Most data indicates that Alzheimer's disease involves an accumulation of amyloid β - peptide (Aβ) in the CNS and that sporadic cases arise from a deficiency in Aβ clearance. Considerable attention has been given to mechanisms by which Aβ might be transported between the brain and blood, and evidence suggests that p-glycoprotein, also known as the multi-drug resistance (MDR) protein (product of the ABCB1 gene), plays a role in Aβ transport across the blood-brain barrier (BBB). We tested this possibility through two approaches: First, wild-type and MDR1A-knockout mice were compared after intravenous injection of [(125)I]-labeled Aβ; after 60 min, homogenates of brain parenchyma were subjected to γ-counting of TCA-precipitable material, and histological sections of brain were subjected to autoradiography...
2016: Current Alzheimer Research
https://www.readbyqxmd.com/read/26940566/inoculation-with-enterococci-does-not-affect-colon-inflammation-in-the-multi-drug-resistance-1a-deficient-mouse-model-of-ibd
#9
Matthew P G Barnett, Yvonne E M Dommels, Christine A Butts, Shuotun Zhu, Warren C McNabb, Nicole C Roy
BACKGROUND: Intestinal bacteria are thought to play a role in the pathogenesis of human inflammatory bowel disease (IBD). We investigated whether oral inoculation with specific intestinal bacteria increased colon inflammation in the multi-drug resistance 1a-deficient (Mdr1a (-/-) ) mouse model of IBD. METHODS: Five-week-old Mdr1a (-/-) mice (FVB background) and FVB mice were randomly assigned to one of two treatment groups (Control or Inoculation, n = 12 per group)...
2016: BMC Gastroenterology
https://www.readbyqxmd.com/read/26924499/-67-68-galmydar-a-metalloprobe-for-monitoring-breast-cancer-resistance-protein-bcrp-mediated-functional-transport-activity
#10
Jothilingam Sivapackiam, Scott E Harpstrite, Julie L Prior, Stephen Mattingly, Vijay Sharma
INTRODUCTION: For stratification of chemotherapeutic choices, radiopharmaceuticals capable of imaging breast cancer resistance protein (BCRP/ABCG2)-mediated functional transport are desired. To accomplish this objective, Galmydar, a fluorescent and moderately hydrophobic Ga(III) cationic complex and its (67/68)Ga-radiolabeled counterparts were interrogated in HEK293 cells stably transfected with BCRP and their WT counterparts transfected with empty vector. Additionally, the sensitivity and specificity of (68)Ga-Galmydar to evaluate functional expression of BCRP at the blood-brain barrier (BBB) was investigated in gene-knockout mdr1a/1b(-/-) (double knockout, dKO) and mdr1a/1b(-/-)ABCG2(-/-) (triple knockout, tKO) mouse models...
March 2016: Nuclear Medicine and Biology
https://www.readbyqxmd.com/read/26830080/investigation-of-utility-of-cerebrospinal-fluid-drug-concentration-as-a-surrogate-for-interstitial-fluid-concentration-using-microdialysis-coupled-with-cisternal-cerebrospinal-fluid-sampling-in-wild-type-and-mdr1a-rats
#11
Yoko Nagaya, Yoshitane Nozaki, Osamu Takenaka, Ryuji Watari, Kazutomi Kusano, Tsutomu Yoshimura, Hiroyuki Kusuhara
In drug discovery, the cerebrospinal fluid (CSF) drug concentration (CCSF) has been used as a surrogate for the interstitial fluid (ISF) concentration (CISF). However, the CCSF-to-CISF gradient suggested for P-glycoprotein (P-gp) substrates in rodents causes uncertainty in CISF estimations and subsequent pharmacokinetic-pharmacodynamic analyses. To evaluate the utility of CCSF as a surrogate for CISF, this study directly compared the CCSF with the CISF of 12 compounds, including P-gp substrates, under steady-state conditions in wild-type and Mdr1a(-/-) rats using microdialysis coupled with cisternal CSF sampling...
February 2016: Drug Metabolism and Pharmacokinetics
https://www.readbyqxmd.com/read/26780497/role-of-p-glycoprotein-in-mediating-rivastigmine-effect-on-amyloid-%C3%AE-brain-load-and-related-pathology-in-alzheimer-s-disease-mouse-model
#12
Loqman A Mohamed, Jeffrey N Keller, Amal Kaddoumi
Recently, we showed that rivastigmine decreased amyloid-β (Aβ) brain load in aged rats by enhancing its clearance across the blood-brain barrier (BBB) via upregulation of P-glycoprotein (P-gp) and low-density lipoprotein receptor-related protein 1 (LRP1). Here, we extend our previous work to clarify P-gp role in mediating rivastigmine effect on Aβ brain levels and neuroprotection in a mouse model of Alzheimer's disease (AD) that expresses different levels of P-gp. APPSWE mice were bred with mdr1a/b knockout mice to produce littermates that were divided into three groups; APP(+)/mdr1(+/+), APP(+)/mdr1(+/-) and APP(+)/mdr1(-/-)...
April 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/26593909/borneol-depresses-p-glycoprotein-function-by-a-nf-%C3%AE%C2%BAb-signaling-mediated-mechanism-in-a-blood-brain-barrier-in-vitro-model
#13
Xiang Fan, Lijuan Chai, Han Zhang, Yuefei Wang, Boli Zhang, Xiumei Gao
P-glycoprotein (P-gp) on brain microvascular endothelial cells (BMECs) that form the blood brain barrier (BBB), influences transportation of substances between blood and brain. The objective of this study was to characterize the effects of borneol on P-gp efflux function on BBB and explore the potential mechanisms. We established an in vitro BBB model comprised of rat BMECs and astrocytes to measure the effects of borneol on the known P-gp substrates transport across BBB, and examined the function and expression of P-gp in BMECs and the signaling pathways regulating P-gp expression...
2015: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/26558156/orally-administered-extract-from-prunella-vulgaris-attenuates-spontaneous-colitis-in-mdr1a-mice
#14
Kelley Mk Haarberg, Meghan J Wymore Brand, Anne-Marie C Overstreet, Catherine C Hauck, Patricia A Murphy, Jesse M Hostetter, Amanda E Ramer-Tait, Michael J Wannemuehler
AIM: To investigate the ability of a Prunella vulgaris (P. vulgaris) ethanolic extract to attenuate spontaneous typhlocolitis in mdr1a(-/-) mice. METHODS: Vehicle (5% ethanol) or P. vulgaris ethanolic extract (2.4 mg/d) were administered daily by oral gavage to mdr1a(-/-) or wild type FVB(WT) mice from 6 wk of age up to 20 wk of age. Clinical signs of disease were noted by monitoring weight loss. Mice experiencing weight loss in excess of 15% were removed from the study...
November 6, 2015: World Journal of Gastrointestinal Pharmacology and Therapeutics
https://www.readbyqxmd.com/read/26557010/novel-understanding-of-abc-transporters-abcb1-mdr-p-glycoprotein-abcc2-mrp2-and-abcg2-bcrp-in-colorectal-pathophysiology
#15
Vibeke Andersen, Katrine Svenningsen, Lina Almind Knudsen, Axel Kornerup Hansen, Uffe Holmskov, Allan Stensballe, Ulla Vogel
AIM: To evaluate ATP-binding cassette (ABC) transporters in colonic pathophysiology as they had recently been related to colorectal cancer (CRC) development. METHODS: Literature search was conducted on PubMed using combinations of the following terms: ABC transporters, ATP binding cassette transporter proteins, inflammatory bowel disease, ulcerative, colitis, Crohn's disease, colorectal cancer, colitis, intestinal inflammation, intestinal carcinogenesis, ABCB1/P-glycoprotein (P-gp/CD243/MDR1), ABCC2/multidrug resistance protein 2 (MRP2) and ABCG2/breast cancer resistance protein (BCRP), Abcb1/Mdr1a, abcc2/Mrp2, abcg2/Bcrp, knock-out mice, tight junction, membrane lipid function...
November 7, 2015: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/26554626/effect-of-subchronic-intravenous-morphine-infusion-and-naloxone-precipitated-morphine-withdrawal-on-p-gp-and-bcrp-at-the-rat-blood-brain-barrier
#16
Catarina Chaves, David Gómez-Zepeda, Sylvain Auvity, Marie-Claude Menet, Dominique Crété, Laurence Labat, Fernando Remião, Salvatore Cisternino, Xavier Declèves
Chronic morphine regimen increases P-glycoprotein (P-gp) and breast cancer-resistance protein (Bcrp) expressions at the rat blood–brain barrier (BBB) but what drives this effect is poorly understood. The objective of this study is to assess subchronic continuous morphine infusion and naloxone-precipitated morphine withdrawal effects on P-gp/Bcrp contents and activities at the rat BBB. Rats were treated either with (i) a continuous i.v. morphine for 120 h, (ii) escalating morphine dosing (10-40 mg/kg, i.p...
January 2016: Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/26460146/increased-intestinal-p-glycoprotein-expression-and-activity-with-progression-of-diabetes-and-its-modulation-by-epigallocatechin-3-gallate-evidence-from-pharmacokinetic-studies
#17
Ranjeet Prasad Dash, Bhanuchander Ellendula, Milee Agarwal, Manish Nivsarkar
The aim of this study was to evaluate the change in the expression and the activity of intestinal P-glycoprotein (efflux transporter) with progression of diabetes in rats. Diabetes was induced in Wistar rats using a combination of low dose streptozotocin along with high fat diet. The expression of intestinal P-glycoprotein significantly increased (P≤0.05) with the progression of diabetes which was inferred from the mRNA analysis of mdr1a and mdr1b genes in the ileum segment of rat intestine. Furthermore, a significant increase (P≤0...
November 15, 2015: European Journal of Pharmacology
https://www.readbyqxmd.com/read/26457007/ccr9-antagonists-in-the-treatment-of-ulcerative-colitis
#18
Pirow Bekker, Karen Ebsworth, Matthew J Walters, Robert D Berahovich, Linda S Ertl, Trevor T Charvat, Sreenivas Punna, Jay P Powers, James J Campbell, Timothy J Sullivan, Juan C Jaen, Thomas J Schall
While it has long been established that the chemokine receptor CCR9 and its ligand CCL25 are essential for the movement of leukocytes into the small intestine and the development of small-intestinal inflammation, the role of this chemokine-receptor pair in colonic inflammation is not clear. Toward this end, we compared colonic CCL25 protein levels in healthy individuals to those in patients with ulcerative colitis. In addition, we determined the effect of CCR9 pharmacological inhibition in the mdr1a(-/-) mouse model of ulcerative colitis...
2015: Mediators of Inflammation
https://www.readbyqxmd.com/read/26454782/5-ht2a-receptor-spect-imaging-with-%C3%A2-%C3%A2-%C3%A2-i-r91150-under-p-gp-inhibition-with-tariquidar-more-is-better
#19
Stergios Tsartsalis, Benjamin B Tournier, Trinh Huynh-Gatz, Noé Dumas, Nathalie Ginovart, Marcelle Moulin-Sallanon, Philippe Millet
INTRODUCTION: Pharmacological P-glycoprotein (P-gp) inhibition with tariquidar (TQD) is considered a promising strategy for the augmentation of radiotracer brain uptake. However, a region-dependent effect may compromise the robustness of quantitative studies. For this reason, we studied the effect of a TQD pretreatment on 5-HT2A imaging with [(123)I]R91150 and compared results with those obtained in Mdr1a knock-out (KO) rats. METHODS: Ex vivo autoradiography was performed in TQD (15 mg/kg) pretreated wild-type (WT-TQD), Mdr1a knock-out (KO) and untreated WT rats for Specific Binding Ratio (SBR) estimation...
January 2016: Nuclear Medicine and Biology
https://www.readbyqxmd.com/read/26438157/efficacy-of-parp-inhibitor-rucaparib-in-orthotopic-glioblastoma-xenografts-is-limited-by-ineffective-drug-penetration-into-the-central-nervous-system
#20
Karen E Parrish, Ling Cen, James Murray, David Calligaris, Sani Kizilbash, Rajendar K Mittapalli, Brett L Carlson, Mark A Schroeder, Julieann Sludden, Alan V Boddy, Nathalie Y R Agar, Nicola J Curtin, William F Elmquist, Jann N Sarkaria
PARP inhibition can enhance the efficacy of temozolomide and prolong survival in orthotopic glioblastoma (GBM) xenografts. The aim of this study was to evaluate the combination of the PARP inhibitor rucaparib with temozolomide and to correlate pharmacokinetic and pharmacodynamic studies with efficacy in patient-derived GBM xenograft models. The combination of rucaparib with temozolomide was highly effective in vitro in short-term explant cultures derived from GBM12, and, similarly, the combination of rucaparib and temozolomide (dosed for 5 days every 28 days for 3 cycles) significantly prolonged the time to tumor regrowth by 40% in heterotopic xenografts...
December 2015: Molecular Cancer Therapeutics
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