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https://www.readbyqxmd.com/read/29329617/dna-detection-of-trypanosoma-evansi-diagnostic-validity-of-a-new-assay-based-on-loop-mediated-isothermal-amplification-lamp
#1
Qunbo Tong, Rui Chen, Qingming Kong, Julie Goossens, Magdalena Radwanska, Di Lou, Jianzu Ding, Bin Zheng, Yixiu Fu, Tianping Wang, Magez Stefan, Shaohong Lu
Trypanosoma evansi (T. evansi) is the most widely spread pathogenic trypanosome in the world. The control of trypanosomiasis depends on accurate diagnosis and effective treatment. Focusing on the presence of T. evansi in Asia, we developed a detection assay based on tracing phosphate ions (Pi) generated during LAMP targeting the variant surface glycoprotein (VSG) gene of Rode Trypanozoon antigenic type 1.2 (RoTat 1.2 VSG). The diagnostic potential as well as the use of the assay as a test-of-cure method after berenil treatment, was assessed in mice at different time points of infection...
January 30, 2018: Veterinary Parasitology
https://www.readbyqxmd.com/read/29311739/application-of-variable-selection-in-the-origin-discrimination-of-wolfiporia-cocos-f-a-wolf-ryvarden-gilb-based-on-near-infrared-spectroscopy
#2
Tianjun Yuan, Yanli Zhao, Ji Zhang, Yuanzhong Wang
Dried sclerotium of Wolfiporia cocos (F.A. Wolf) Ryvarden & Gilb. is a traditional Chinese medicine. Its chemical components showed difference among geographical origins, which made it difficult to keep therapeutic potency consistent. The identification of the geographical origin of W. cocos is the fundamental prerequisite for its worldwide recognition and acceptance. Four variable selection methods were employed for near infrared spectroscopy (NIR) variable selection and the characteristic variables were screened for the establishment of Fisher function models in further identification of the origin of W...
January 8, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29301952/the-deubiquitinase-otu5-regulates-root-responses-to-phosphate-starvation
#3
Der-Fen Suen, Yi-Hsiu Tsai, Ya-Tan Cheng, Ramalingam Radjacommare, Ram Nivas Ahirwar, Hongyong Fu, Wolfgang Schmidt
Phosphorus, taken up by plants as inorganic phosphate (Pi), is an essential but often growth-limiting mineral nutrient for plants. As part of an orchestrated response to improve its acquisition, insufficient Pi supply triggers alterations in root architecture and epidermal cell morphogenesis. Mutants defective in the expression of the OVARIAN TUMOR DOMAIN-CONTAINING DEUBIQUITINATING ENZYME5 (OTU5) exhibited a constitutive Pi deficiency root phenotype, comprising the formation of long and dense root hairs and attenuated primary root growth...
January 4, 2018: Plant Physiology
https://www.readbyqxmd.com/read/29203613/nfe2l1-silenced-insulinoma-cells-acquire-aggressiveness-and-chemo-resistance
#4
Jingqi Fu, Hongzhi Zheng, Qi Cui, Chengjie Chen, Simeng Bao, Jing Sun, Lu Li, Bei Yang, Huihui Wang, Yongyong Hou, Yuanyuan Xu, Yuanhong Xu, Qiang Zhang, Jingbo Pi
The transcription factor nuclear factor erythroid 2 like 1 (NFE2L1 or NRF1) is involved in various critical cell processes such as maintenance of ubiquitin-proteasome system and regulation of the cellular antioxidant response. We previously determined that pancreatic β-cell-specific Nfe2l1-knockout mice had hyperinsulinemia, and that silencing of Nfe2l1 in mouse islets or MIN6 insulinoma β-cells induced elevated basal insulin release and altered glucose metabolism. Hypoglycemia is a major issue with aggressive insulinomas, although a role of NFE2L1 in this pathology is not defined...
December 4, 2017: Endocrine-related Cancer
https://www.readbyqxmd.com/read/29183726/gsdme-mediates-caspase-3-dependent-pyroptosis-in-gastric-cancer
#5
Yubin Wang, Bo Yin, Dinuo Li, Guijun Wang, Xiangdong Han, Xuejun Sun
Gastric cancer is a malignancy that starts from the cells in the stomach with relatively low overall survival rate. Chemotherapy following resection surgery has been recommended as a curative strategy for gastric cancer. However, the mechanism of the chemotherapy drugs on gastric cancer is not completely understood. Pyroptosis is a form of programmed cell death and plays critical role in immunity. The role of pyroptosis on cancer cells is less known. In this study, we treated SGC-7901 and MKN-45 with 5-FU and found that the cell viability was significantly decreased...
November 25, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29151957/curcumin-enhances-the-anticancer-effect-of-5-fluorouracil-against-gastric-cancer-through-down-regulation-of-cox-2-and-nf-%C3%AE%C2%BAb-signaling-pathways
#6
Hongru Yang, Shaoqiu Huang, Yumeng Wei, Shousong Cao, Chao Pi, Ting Feng, Jing Liang, Ling Zhao, Guosheng Ren
Background: 5-fluorouracil (5-FU) is one of the most commonly used first-line anticancer drugs to treat gastric cancer in clinical practice. However, severe adverse events such as gastrointestinal toxicity and bone marrow suppression limit its clinical application. Combination chemotherapy to combine two or more anticancer drugs with different mechanistic action is an effective anticancer strategy against gastric cancer. Therefore, we studied the anticancer effect of the combination of 5-FU with curcumin against gastric cancer MKN45 and AGS cells (normal gastric mucosal GES-1 cells as control) and associated molecular mechanisms...
2017: Journal of Cancer
https://www.readbyqxmd.com/read/29136743/-drug-resistance-mutations-and-its-associated-factors-among-579-hiv-aids-patients-experiencing-failure-of-antiretroviral-therapy-in-jiangsu-province-china
#7
P P Xiao, J Lu, H Y Hu, X Q Xu, P Ding, G F Fu, X P Huan, Y Zhou, H T Yang
Objective: To understand the incidence and related factors of HIV-1 drug resistance among HIV/AIDS patients experiencing treatment failure in Jiangsu province, China. Methods: The HIV/AIDS integrated prevention and control data information management system of China were used to collect the basic data of patients, blood specimens were collected from patients who had antiretroviral therapy (ART) failure with ≥12 months and older than 18 years in 2016 in Jiangsu, excluding cases with missing information, 713 cases were enrolled in this study...
November 6, 2017: Zhonghua Yu Fang Yi Xue za Zhi [Chinese Journal of Preventive Medicine]
https://www.readbyqxmd.com/read/29126308/nrf2-deficiency-promotes-the-progression-from-acute-tubular-damage-to-chronic-renal-fibrosis-following-unilateral-ureteral-obstruction
#8
Weiwei Kong, Jingqi Fu, Nan Liu, Congcong Jiao, Guangying Guo, Junjun Luan, Huihui Wang, Li Yao, Lining Wang, Masayuki Yamamoto, Jingbo Pi, Hua Zhou
Background: Nuclear factor erythroid 2-related factor 2 (Nrf2) is a central mediator of cellular responses to oxidative stress. We hypothesized that Nrf2 modulates progression from acute tubular damage to renal fibrosis. We asked whether Nrf2 deletion increases renal injury in mice following unilateral ureteral obstruction (UUO). Methods: We explored the time course of renal injury and Nrf2 expression in Nrf2+/+ mice following UUO. We compared Nrf2+/+ and Nrf2-/- mice following UUO in tubular damage, transdifferentiation [vimentin, proliferating cell nuclear antigen (PCNA)], fibrosis [fibronectin, α-smooth muscle actin (SMA)], antioxidative and inflammatory responses...
November 6, 2017: Nephrology, Dialysis, Transplantation
https://www.readbyqxmd.com/read/29124255/a-microdialysis-based-analytical-system-for-dynamic-monitoring-of-arsenic-transformation-under-microbial-activity
#9
Keng-Chang Hsu, Jing-Ru Hsieh, Ya-Ching Chen, Pi-Fu Hsu, Chih-Chang Hung, Yeou-Lih Huang
In this study, a microdialysis (MD) technique was combined with high-performance liquid chromatography/inductively coupled plasma mass spectrometry (HPLC-ICP-MS) for continuous monitoring of the dynamic variations of arsenic species in a microbe-inoculated culture broth. After optimizing the analytical system, retrodialysis was applied to assess the probe recovery and analytical performance of the proposed method. The mean probe recoveries of four arsenic species were in the range from 57.0 to 81.8%. The limit of detection (LOD) for arsenic was in the range of 0...
November 10, 2017: Analyst
https://www.readbyqxmd.com/read/29081891/nrf2-is-a-potential-modulator-of-hyperresistance-to-arsenic-toxicity-in-stem-like-keratinocytes
#10
Xiafang Wu, Bei Yang, Yuxin Hu, Ru Sun, Huihui Wang, Jingqi Fu, Yongyong Hou, Jingbo Pi, Yuanyuan Xu
Arsenic is a well-known human carcinogen. Stem cells are indicated to be involved in arsenic carcinogenesis and have a survival selection advantage during arsenic exposure with underlying mechanisms undefined. In the present study, we demonstrated that CD34(high)-enriched cells derived from HaCaT human keratinocytes showed stem-like phenotypes. These cells were more resistant to arsenic toxicity and had higher arsenic efflux ability than their mature compartments. The master transcription factor in antioxidant defense, nuclear factor erythroid 2-related factor 2 (NRF2) with its downstream genes, was highly expressed in CD34(high)-enriched cells...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/29064419/ni-ii-complexes-with-schiff-base-ligands-preparation-characterization-dna-protein-interaction-and-cytotoxicity-studies
#11
Hui Yu, Wei Zhang, Qing Yu, Fu-Ping Huang, He-Dong Bian, Hong Liang
In this study, two Ni(II) complexes, namely [Ni(HL1)₂(OAc)₂] (1) and [Ni(L2)₂] (2) (where HL1 and HL2 are (E)-1-((1-(2-hydroxyethyl)-1H-pyrazol-5-ylimino)methyl)-naphthalen-2-ol) and (E)-ethyl-5-((2-hydroxynaphthalen-1-yl)methyleneamino)-1-methyl-1H-pyrazole-4-carboxylate, respectively), were synthesized and characterized by X-ray crystallography, Electrospray Ionization Mass Spectrometry (ESI-MS), elemental analysis, and IR. Their uptake in biological macromolecules and cancer cells were preliminarily investigated through electronic absorption (UV-Vis), circular dichroism (CD) and fluorescence quenching measurements...
October 24, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29061907/deubiquitllating-enzyme-otu5-contributes-to-dna-methylation-patterns-and-is-critical-for-phosphate-nutrition-signals
#12
Ming-Ren Yen, Der-Fen Suen, Fei-Man Hsu, Yi-Hsiu Tsai, Hongyong Fu, Wolfgang Schmidt, Pao-Yang Chen
Phosphate (Pi) starvation induces a suite of adaptive responses aimed at recalibrating cellular Pi homeostasis. Plants harboring a mutation in OVARIAN TUMOR DOMAIN-CONTAINING DEUBIQUITILATING ENZYME5 (OTU5) showed altered DNA methylation of root hair-related genes and altered Pi-responsive root traits. Unlike the wild type, homozygous otu5 mutants did not respond to Pi starvation by increased lateral root formation and increased root hair length, but formed very short root hairs when grown on low Pi media. Under Pi-replete conditions, otu5 plants developed more root hairs than the wild type due to attenuated primary root growth, a phenotype which resembled that of Pi-deficient plants...
October 23, 2017: Plant Physiology
https://www.readbyqxmd.com/read/29040392/%C3%AE-cypermethrin-and-its-metabolite-3-phenoxybenzoic-acid-exhibit-immunotoxicity-in-murine-macrophages
#13
Xia Wang, Bingnan He, Baida Kong, Lai Wei, Rong Wang, Chenqian Zhou, Yiyan Shao, Jiajia Lin, Yuanxiang Jin, Zhengwei Fu
β-Cypermethrin (β-CYP), one of most important pyrethroids, is widely used to control insects, and has been detected in organisms, including human. Pyrethroids have been shown to pose neurotoxicity, hepatotoxicity, endocrine disruption and reproductive risks in mammals. However, research in immunotoxicity of pyrethroids, especially their metabolites, is limited. A common metabolite of pyrethroids is 3-phenoxybenzoic acid (3-PBA) in mammals. Thus, in this study, we evaluated the immunotoxicity of β-CYP and 3-PBA in mouse macrophages, RAW 264...
October 11, 2017: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/29035339/promoting-effect-of-pinostrobin-on-the-proliferation-differentiation-and-mineralization-of-murine-pre-osteoblastic-mc3t3-e1-cells
#14
Chengbo Gu, Linan Fu, Xiaohan Yuan, Zhiguo Liu
Pinostrobin (PI), a natural flavonoid found in a variety of plants, is well known for its rich pharmacological activities. However, its osteogenic function remains unclear. The aim of this study is to evaluate the effect of PI on the proliferation, differentiation, and mineralization of murine pre-osteoblastic MC3T3-E1 cells in vitro using MTT, alkaline phosphatase (ALP) activity, the synthesis of collagen I (Col I) assay, and Von-Kossa staining, respectively. The expression of osteocalcin (OCN) mRNA in cells was detected by real-time PCR...
October 16, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29016433/under-correction-of-sagittal-deformities-based-on-age-adjusted-alignment-thresholds-leads-to-worse-hrqol-while-over-correction-provides-no-additional-benefit
#15
Justin K Scheer, Renaud Lafage, Frank J Schwab, Barthelemy Liabaud, Justin S Smith, Gregory M Mundis, Richard Hostin, Christopher I Shaffrey, Douglas C Burton, Robert A Hart, Han Jo Kim, Shay Bess, Munish Gupta, Virginie Lafage, Christopher P Ames
STUDY DESIGN: Retrospective review of prospectively-collected database. OBJECTIVE: This study aims to compare 2-year clinical outcomes of patients who underwent surgical reconstructions based on their achievement to age-adjusted alignment ideals. SUMMARY OF BACKGROUND DATA: Recent research in sagittal plane has proposed age-adjusted alignment thresholds. However, the impact of these thresholds on post-operative HRQL is yet to be investigated...
October 9, 2017: Spine
https://www.readbyqxmd.com/read/28989847/comparison-between-s2-alar-iliac-screw-fixation-and-iliac-screw-fixation-in-adult-deformity-surgery-reoperation-rates-and-spinopelvic-parameters
#16
Wataru Ishida, Benjamin D Elder, Christina Holmes, Sheng-Fu L Lo, C Rory Goodwin, Thomas A Kosztowski, Ali Bydon, Ziya L Gokaslan, Jean-Paul Wolinsky, Daniel M Sciubba, Timothy F Witham
STUDY DESIGN: Retrospective cohort study. OBJECTIVE: The S2-alar-iliac (S2AI) technique has been described as an alternative method for pelvic fixation in place of iliac screws (ISs) in spinal deformity surgery. The objective of this study was to analyze the impact of S2AI screws on radiographical outcomes, including spinopelvic parameters. METHODS: A retrospective review of 17 patients receiving ISs and 46 patients receiving S2AI screws for correction of adult spinal deformity between 2010 and 2015 with minimum 1-year follow-up was conducted...
October 2017: Global Spine Journal
https://www.readbyqxmd.com/read/28983470/the-espf-n-terminal-of-enterohemorrhagic-escherichia-coli-o157-h7-edl933w-imparts-stronger-toxicity-effects-on-ht-29-cells-than-the-c-terminal
#17
Xiangyu Wang, Yanli Du, Ying Hua, Muqing Fu, Cong Niu, Bao Zhang, Wei Zhao, Qiwei Zhang, Chengsong Wan
Enterohemorrhagic Escherichia coli (EHEC) O157:H7 EspF is an important multifunctional protein that destroys the tight junctions of intestinal epithelial cells and promotes host cell apoptosis. However, its molecular mechanism remains elusive. We knocked out the espF sequence (747 bp, ΔespF), N-terminal sequence (219 bp, ΔespFN ), and C-terminal sequence (528 bp, ΔespFC ) separately using the pKD46-mediated λ Red homologous recombination system. Then, we built the corresponding complementation strains, namely, ΔespF/pespF, ΔespFN/pespFN , and ΔespFC/pespFC by overlap PCR, which were used in infecting HT-29 cells and BALB/C mice...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28943966/ligands-of-the-peroxisome-proliferator-activated-receptor-%C3%AE-inhibit-hepatoce-llular-carcinoma-cell-proliferation
#18
Zhe Jin, Baoxing Jia, Yu Fu, Ludong Tan, Qingmin Chen, Peiqiang Jiang, Yahui Liu
This study was designed to investigate the regulatory role of the peroxisome proliferator-activated receptor γ (PPARγ) in the growth of hepatocellular carcinoma cells under the hypothesis that the levels of the phosphatase and tensin homologue deleted on chromosome 10 (PTEN) mRNA and the phosphorylated Akt (pAkt) protein would be affected by the presence of different receptor ligand concentrations. SMMC-7721 hepatocellular carcinoma cells were cultured in the presence of different concentrations of either 15-deoxyprostaglandin J2 (15-d-PGJ2) or pioglitazone and experiments were conducted in order to determine cell growth changes and measure levels of PTEN mRNA and pAkt protein...
October 2017: Oncology Letters
https://www.readbyqxmd.com/read/28930181/ambient-air-pollution-and-risk-for-ischemic-stroke-a-short-term-exposure-assessment-in-south-china
#19
Pi Guo, Yulin Wang, Wenru Feng, Jiagang Wu, Chuanxi Fu, Hai Deng, Jun Huang, Li Wang, Murui Zheng, Huazhang Liu
Data on the association between air pollution and risk of ischemic stroke in China are still limited. This study aimed to investigate the association between short-term exposure to ambient air pollution and risk of ischemic strokes in Guangzhou, the most densely-populated city in south China, using a large-scale multicenter database of stroke hospital admissions. Daily counts of ischemic stroke admissions over the study years 2013-2015 were obtained from the Guangzhou Cardiovascular and Cerebrovascular Disease Event Surveillance System...
September 20, 2017: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/28901461/mir106b-regulates-retinoblastoma-y79-cells-through-runx3
#20
Ge Yang, Yang Fu, Luxi Zhang, Xiaoyan Lu, Qiuming Li
MicroRNAs are increasingly recognized as important regulators of cancer. The aim of the present study was to investigate the role of miR-106b in the regulation of Y79 retinoblastoma. Y79 cells were transfected with antisense oligonucleotides (ASO) against miR-106b (ASO-miR-106b) or ASO-control. After transfection, the levels of miR-106b were monitored with real-time PCR (RT-PCR). The effects of ASO-miR-106b transfection on cell viability was evaluated by Cell Counting Kit-8 (CCK-8) analysis at 24, 48 and 72 h after transfection...
November 2017: Oncology Reports
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