Read by QxMD icon Read


Christina Caroline Plaschke, Anita Gothelf, Julie Gehl, Irene Wessel
BACKGROUND: Electrochemotherapy, the combination of electroporation and chemotherapy, is mainly used in the palliative setting for treatment of cutaneous and subcutaneous metastases; however, new applications are continuously being explored. Patients with head and neck cancer are primarily treated with surgery and/or radio-chemotherapy. In the setting of local recurrence with no further curative treatment options available, electrochemotherapy could be of value. We therefore performed a systematic search of the present literature...
October 5, 2016: Acta Oncologica
A L Tosi, L G Campana, F Dughiero, M Forzan, M Rastrelli, E Sieni, C R Rossi
Tissue electrical conductivity is correlated with tissue characteristics. In this work, some soft tissue sarcomas (STS) excised from patients have been evaluated in terms of histological characteristics (cell size and density) and electrical resistance. The electrical resistance has been measured using the ex vivo study on soft tissue tumors electrical characteristics (ESTTE) protocol proposed by the authors in order to study electrical resistance of surgical samples excised by patients in a fixed measurement setup...
October 1, 2016: Medical & Biological Engineering & Computing
Simone Ribero, Eugenio Sportoletti Baduel, Matteo Brizio, Franco Picciotto, Emi Dika, Maria Teresa Fierro, Giuseppe Macripò, Pietro Quaglino
BACKGROUND: Metastatic extraorbital sebaceous carcinoma is a rare event that could involve the head and neck. The treatment of choice for the initial stage of the disease is surgery and/or radiotherapy. The treatment of recurrent or advanced disease is still controversial. MATERIAL AND METHODS: Extensive literature search was done, and the treatment options are discussed. RESULTS: Results. The literature search found several treatment modalities in use for the treatment of metastatic extraorbital sebaceous carcinoma...
September 1, 2016: Radiology and Oncology
Tanja Dolinsek, Lara Prosen, Maja Cemazar, Tjasa Potocnik, Gregor Sersa
BACKGROUND: The aim of the study was to explore the effectiveness of electrochemotherapy (ECT) during the treatment of melanoma patients with BRAF inhibitors. Its effectiveness was tested on BRAF mutated and non-mutated melanoma cells in vitro and in combination with BRAF inhibitors. MATERIALS AND METHODS: ECT with bleomycin was performed on two human melanoma cell lines, with (SK-MEL-28) or without (CHL-1) BRAF V600E mutation. Cell survival was determined using clonogenic assay to determine the effectiveness of ECT in melanoma cells of different mutation status...
September 1, 2016: Radiology and Oncology
Tadeja Forjanič, Damijan Miklavčič
The effectiveness of electrochemotherapy, a local treatment using electric pulses to increase the uptake of chemotherapeutic drug, includes several antitumor mechanisms. In addition to the cytotoxic action of chemotherapeutic drug, treatment outcome also depends on antitumor immune response. In order to assess the contribution of different antitumor mechanisms to the observed treatment outcome, we designed a model of tumor volume dynamics, which is able to quantify early and late treatment effects. Model integrates characteristics of both main posttreatment processes, namely removal of lethally damaged cells from tumor volume and tumor-immune interaction...
September 22, 2016: Medical & Biological Engineering & Computing
Anna Zygogianni, George Kyrgias, John Scarlatos, Michael Koukourakis, Kyriakos Souliotis, John Kouvaris, Nikolaos Kelekis, Vassilis Kouloulias
BACKGROUND: The aim of this study was to investigate whether electrochemotherapy is a clinically and cost-effective treatment option against skin tumors. MATERIALS AND METHODS: We performed an analysis of the current literature based on database searches in PubMed/MEDLINE and we included articles till July 2012. Terms used for the search were 'electrochemotherapy', 'skin cancer', 'recurrence', and 'cutaneous and subcutaneous tumors'. Only papers published in English were included...
2016: Asian Pacific Journal of Cancer Prevention: APJCP
Gian M Tomassini, Piero Covarelli, Marco A Tomassini, Alessia Corsi, Leonardo Bianchi, Katharina Hansel, Luca Stingeni
BACKGROUND: The treatment of cutaneous advanced non-melanoma skin cancers (NMSC) and multiple cutaneous and subcutaneous melanoma metastases (Mm) represents a main therapeutic challenge. Electrochemotherapy (ECT) is an anticancer procedure that facilitates the penetration of cytotoxic drugs into cancer cells by means of the application of electrical pulses. The aim of our study was to evaluate efficacy and tolerability of ECT in the treatment of advanced NMSC and Mm. METHODS: Thirteen patients with a total of 88 cutaneous and subcutaneous tumour lesions were selected (69 Mm in 6 patients and 19 NMSC in 7 patients)...
October 2016: Giornale Italiano di Dermatologia e Venereologia: Organo Ufficiale, Società Italiana di Dermatologia e Sifilografia
Tina Kosjek, Anja Krajnc, Tjasa Gornik, Dusan Zigon, Ales Groselj, Gregor Sersa, Maja Cemazar
Bleomycin is a cytotoxic antibiotic available as a compost of structurally strongly related glycopeptides, which is in vivo found chelated with several metals. Its pharmacotherapy has merely been based on experimental dose - response data, whereas its biodistribution and pharmacokinetics remain fundamentally unknown. This is reasoned by an absence of a specific and sensitive mass spectrometry-based analytical method for its determination in biological tissues. We herein reveal the results of our study on the mass spectrometric behavior of two main bleomycin fractions A2 and B2, including their metal complexes, particularly the predominant copper chelates...
November 1, 2016: Talanta
Luca Giovanni Campana, Roberto Marconato, Elisabetta Sieni, Sara Valpione, Luigi Corti, Simone Mocellin, Carlo Riccardo Rossi
Electrochemotherapy (ECT) has emerged among European centers as an innovative locoregional treatment for patients with unresectable skin cancers or superficial metastases from any histotype. The combined administration of a cytotoxic agent (bleomycin or cisplatin) with properly tuned electric pulses results in locally-enhanced drug delivery (reversible electroporation) into malignant cells and sustained tumor response. Reversible electroporation represents the basis of ECT and allows the potentiation of two low permeant cytotoxic agents such as bleomycin (~8000 fold) and cisplatin (~80 fold)...
August 2016: Recenti Progressi in Medicina
P Di Gennaro, G Gerlini, C Urso, S Sestini, P Brandani, N Pimpinelli, L Borgognoni
Electrochemotherapy (ECT) represents an effective local treatment for skin unresectable melanoma metastases with high overall objective response rate. ECT is based on the combination of anti-neoplastic drugs administration and cancer cells electroporation. Whether ECT can also activate the immune system is a matter of debate, however a significant recruitment of dendritic cells in melanoma treated metastases has been described. Herein we investigated immediate and late effects of ECT treatment on T cell subsets in ECT-treated lesions by fluorescent immunohistochemistry...
July 30, 2016: Clinical & Experimental Metastasis
Sebastian Theurich, Sacha I Rothschild, Michael Hoffmann, Mario Fabri, Andrea Sommer, Maria Garcia-Marquez, Martin Thelen, Catherine Schill, Ramona Merki, Thomas Schmid, Dieter Koeberle, Alfred Zippelius, Christian Baues, Cornelia Mauch, Christian Tigges, Alexander Kreuter, Jan Borggrefe, Michael von Bergwelt-Baildon, Max Schlaak
Immune checkpoint inhibition with ipilimumab has revolutionized cancer immunotherapy and significantly improved outcomes of patients with advanced malignant melanoma. Local peripheral treatments (LPT), such as radiotherapy or electrochemotherapy, have been shown to modulate systemic immune responses, and preliminary data have raised the hypothesis that the combination of LPT with systemic immune checkpoint blockade might be beneficial. Clinical data from 127 consecutively treated melanoma patients at four cancer centers in Germany and Switzerland were analyzed...
September 2, 2016: Cancer Immunology Research
Enrico P Spugnini, Stefano Fais, Tommaso Azzarito, Alfonso Baldi
Electrochemotherapy (ECT) is a medical strategy that allows an increased efficacy of chemotherapy agents after the application of permeabilizing electric pulses having appropriate characteristics (form, voltage, frequency). In the past ten years, the clinical efficacy of this therapeutic approach in several spontaneous models of tumors in animals has been shown. Moreover, some of the molecular and cellular mechanisms responsible for this phenomenon have been elucidated. Our group has been deeply involved in the development of new ECT protocols for companion animals, implementing the use of the technique as first line treatment, and evaluating different chemotherapy agents in laboratory animals as well as pets...
July 28, 2016: Journal of Cellular Physiology
Janja Dermol, Olga N Pakhomova, Andrei G Pakhomov, Damijan Miklavčič
Electroporation-induced cell sensitization was described as the occurrence of a delayed hypersensitivity to electric pulses caused by pretreating cells with electric pulses. It was achieved by increasing the duration of the electroporation treatment at the same cumulative energy input. It could be exploited in electroporation-based treatments such as electrochemotherapy and tissue ablation with irreversible electroporation. The mechanisms responsible for cell sensitization, however, have not yet been identified...
2016: PloS One
Giuseppe Bianchi, Laura Campanacci, Mattia Ronchetti, Davide Donati
INTRODUCTION: Bone metastatic disease is a major cause of pain and decreased quality of life in patients with cancer. In addition to systemic therapy and pain control with narcotic analgesics, standard local treatments include palliation with radiation therapy and surgery. However, 20-30 % of patients do not respond to conventional treatments, increasing the interest in alternative therapies. We present the results of a new minimally invasive technique in the treatment of bone metastases...
July 21, 2016: World Journal of Surgery
L G Campana, A Testori, P Curatolo, P Quaglino, S Mocellin, M Framarini, L Borgognoni, P A Ascierto, N Mozzillo, M Guida, S Bucher, R Rotunno, F Marenco, G L De Salvo, A De Paoli, C R Rossi, A Bonadies
BACKGROUND: Cutaneous metastases represent a therapeutic challenge. An increasing body of experience suggests that electrochemotherapy (ECT) provides effective tumor control, although its evidence basis should be strengthened. METHODS: This prospective, multicenter, observational study enrolled patients with superficial metastases, who underwent ECT at 10 centers between 2008 and 2013. Outcomes included adherence to European Standard Operating Procedures of ECT (ESOPE), tumor response, local progression-free survival (LPFS), toxicity and patient-reported outcomes (PROs, EORTC QLQ-C30 plus an 8-item questionnaire)...
June 29, 2016: European Journal of Surgical Oncology
Mauro Montuori, Letizia Santurro, Andrea Feliziani, Flavio DE Sanctis, Edoardo Ricciardi, Daniele Gaudio, Elena Campione, Luca Bianchi, Maria B Silvi, Piero Rossi
BACKGROUND: To evaluate feasibility, safety and efficacy in day surgery of Electrochemotherapy using Bleomycin in non melanoma Head and Neck skin cancer. METHODS: We performed a prospective study on fifteen patients treated at the General Surgery Department at University Hospital of Tor Vergata, Rome between April 2011 and February 2013. RESULTS: We observed 100% Complete Response evaluated with WHO criteria at 6 weeks after treatment. No partial response was achieved...
July 5, 2016: Giornale Italiano di Dermatologia e Venereologia: Organo Ufficiale, Società Italiana di Dermatologia e Sifilografia
Evie C Knight, Michael A Shipstone
BACKGROUND: Canine eosinophilic granuloma (CEG) is an uncommon disease. Lesions are typically located in the oral cavity and other cutaneous sites, but are rarely reported to affect the digits. The majority of cases are treated with prednisolone as a monotherapy; alternative treatment options include corticosteroids administered in combination with azathioprine, antihistamines, electrochemotherapy with bleomycin, and surgical resection. Neither chlorambucil nor laser previously have been reported as treatments...
October 2016: Veterinary Dermatology
Daniel C Sweeney, Matej Reberšek, Janja Dermol, Lea Rems, Damijan Miklavčič, Rafael V Davalos
High frequency bipolar electric pulses have been shown to mitigate undesirable muscle contraction during irreversible electroporation (IRE) therapy. Here we evaluate the potential applicability of such pulses for introducing exogenous molecules into cells, such as in electrochemotherapy (ECT). For this purpose we develop a method for calculating the time course of the effective permeability of an electroporated cell membrane based on real-time imaging of propidium transport into single cells that allows a quantitative comparison between different pulsing schemes...
June 29, 2016: Biochimica et Biophysica Acta
Markus V Heppt, Thomas K Eigentler, Katharina C Kähler, Rudolf A Herbst, Daniela Göppner, Thilo Gambichler, Jens Ulrich, Edgar Dippel, Carmen Loquai, Beatrice Schell, Bastian Schilling, Susanne G Schäd, Erwin S Schultz, Fanny Matheis, Julia K Tietze, Carola Berking
Growing evidence suggests that concurrent loco-regional and systemic treatment modalities may lead to synergistic anti-tumor effects in advanced melanoma. In this retrospective multicenter study, we evaluate the use of electrochemotherapy (ECT) combined with ipilimumab or PD-1 inhibition. We investigated patients with unresectable or metastatic melanoma who received the combination of ECT and immune checkpoint blockade for distant or cutaneous metastases within 4 weeks. Clinical and laboratory data were collected and analyzed with respect to safety and efficacy...
August 2016: Cancer Immunology, Immunotherapy: CII
S Ribero, E Balagna, E Sportoletti Baduel, F Picciotto, M Sanlorenzo, M T Fierro, P Quaglino, G Macripo
Generalized eruptive keratoacanthoma, is considered a serious condition because the eruptions are diffuse, persistent, and recurrent. Constant pruritus, visceral neoplasms, and unsatisfactory response to treatment are ominous prognostic factors. Even if skin cancers are usually well controlled with surgical and/or radiotherapic approaches, there are some cases in which these two techniques are not effective. With respect to surgery, radiotherapy and other standard treatments, ECT acts rapidly on multiple lesions with limited side effects and no functional impairment; moreover, repeated sessions can be performed to achieve or maintain the clinical response...
June 6, 2016: Dermatologic Therapy
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"