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Immature platelet

Mohammad-Hossein Beigi, Atefeh Atefi, Hamid-Reza Ghanaei, Sheyda Labbaf, Fatemeh Ejeian, Mohammad-Hossein Nasr-Esfahani
In the current study the effect of superimposing platelet-rich plasma (PRP) on different culture mediums in a three-dimensional (3D) alginate scaffold encapsulated with adipose mesenchymal stem cells (ADSCs) for cartilage tissue repair is reported. The 3D alginate scaffolds with co-administration of PRP and/or chondrogenic supplements had a significant effect on the differentiation of ADSCs into mature cartilage, as assessed by evaluation of the expression of cartilage related markers of Sox9, Collagen II, Aggrecan and collagen and glycosaminoglycan assays...
March 9, 2018: Journal of Tissue Engineering and Regenerative Medicine
Hepeng Fu, Yuxiang Zhang
Platelets play a pivotal role in the thromboembolic, inflammatory, and immunomodulatory process. The alteration of platelet quality often affects the treatment and prognosis of critically ill patients, and has a significant correlation with mortality. With the further research on the function of the platelet, it is found that the abnormal platelet quality can present during the early stage of the illness of the critically ill patients. In order to evaluate the alterations of the platelet quality more accurately, the further studies of platelet parameters, including platelet counts (PLT), platelet hematocrit (PCT), platelet large cell ratio (PLCR), mean platelet volume (MPV), platelet distribution width (PDW), immature platelet fraction (IPF) and so on, are still be the focus of current researches in the field of critical illness...
March 2018: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
Joanna Drozd-Sokołowska, Krzysztof Mądry, Anna Waszczuk-Gajda, Przemysław Biecek, Paweł Szwedyk, Katarzyna Budziszewska, Magdalena Raźny, Magdalena Dutka, Agata Obara, Ewa Wasilewska, Krzysztof Lewandowski, Agnieszka Piekarska, Grażyna Bober, Helena Krzemień, Beata Stella-Hołowiecka, Katarzyna Kapelko-Słowik, Waldemar Sawicki, Małgorzata Paszkowska-Kowalewska, Rafał Machowicz, Jadwiga Dwilewicz-Trojaczek
Atypical chronic myeloid leukaemia (aCML) belongs to myelodysplastic/myeloproliferative neoplasms. Because of its rarity and changing diagnostic criteria throughout subsequent classifications, data on aCML are very scarce. Therefore, we at the Polish Adult Leukemia Group performed a nationwide survey on aCML. Eleven biggest Polish centres participated in the study. Altogether, 45 patients were reported, among whom only 18 patients (40%) fulfilled diagnostic criteria. Among misdiagnosed patients, myelodysplastic/myeloproliferative syndrome unclassifiable and chronic myelomonocytic leukaemia were the most frequent diagnoses...
March 7, 2018: Hematological Oncology
Claudia E Imperiali, Ariadna Arbiol-Roca, Lourdes Sanchez-Navarro, Macarena Dastis-Arias, Juan C Lopez-Delgado, Anna Cortes-Bosch, Ana Sancho-Cerro, Dolors Dot-Bach
Introduction: The Sysmex XN-series haematology analyser has newly adopted a fluorescent channel to measure immature platelet fraction (IPF). To promote the clinical utility of this promising parameter, establishing a reliable reference interval is mandatory. According to previous studies, IPF values may be affected by the employed analyser and the ethnic background of the individual, but no differences seem to be found between individuals' genders. Therefore, this study aimed to define the reference interval for IPF in a Spanish population following Clinical and Laboratory Standard Institute (CLSI) guidelines...
February 15, 2018: Biochemia Medica: časopis Hrvatskoga Društva Medicinskih Biokemičara
Philip Rauber, Frank Lammert, Katharina Grotemeyer, Beate Appenrodt
BACKGROUND AND AIMS: Thrombocytopenia occurs frequently in patients with cirrhosis. The immature platelet fraction (IPF%) is measured to differentiate the causes of thrombocytopenia. To date the relevance of thrombopoietin (TPO) in the context of cirrhosis is unknown. The aim of our study was to investigate the cause of thrombocytopenia in patients with liver cirrhosis by measuring IPF%, TPO and spleen size. In addition we examined the use of IPF% to evaluate the severity of cirrhosis and its complications...
2018: PloS One
Matthew J Flick, Joseph S Palumbo
Platelets were first implicated in the pathogenesis of cancer and inflammatory pathologies decades ago, but an understanding of the mechanisms coupling platelet function to cancer progression is still a work in progress. In their paper, Servais et al. push the field forward by illustrating that platelets represent a crucial link between inflammatory disease and tumorigenesis [1]. This interesting paper presents data showing that platelets promote intestinal tumorigenesis in the context of inflammatory colitis by supporting the recruitment and/or expansion of myeloid-derived suppressor cells (MDSCs)...
February 8, 2018: Journal of Thrombosis and Haemostasis: JTH
Sirong He, Chengshi Wang, Xiaojiong Du, Younan Chen, Jiuming Zhao, Bole Tian, Huimin Lu, Yi Zhang, Jingping Liu, Guang Yang, Lan Li, Hongxia Li, Jingqiu Cheng, Yanrong Lu
Deficient insulin secretion caused by immaturity is the predominant disadvantage of neonatal porcine islets (NPIs) when they serve as a source for islet xenotransplantation. We hypothesize that the transplantation of NPIs with a combination of mesenchymal stem cells (MSCs) can accelerate NPI maturation and improve the engraftment and function of NPIs. After indirect coculturing with monkey MSCs over 21 d, insulin secretion and the expression of regulatory genes relevant to development were assessed in NPIs...
January 18, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
Lianhua He, Chunfang Liu, Congcong Sun, Jingxia Wang, Kai Zhi, Danni Sun, Hui Wang, Qianqian Wang, Na Lin
Wu-tou decoction (WTD) is a classic traditional Chinese medicine formula and has been extensively used for the treatment of rheumatoid arthritis (RA). Previous reports indicates that WTD possesses anti-inflammatory and antinociceptive activities, and inhibits the development of arthritic joints and disease severity of collagen-induced arthritis (CIA) or adjuvant-induced rats; however, its action on angiogenesis of RA has not been clarified. The present study aims to determine the anti-angiogenic activity of WTD in CIA rats and in various angiogenesis models...
January 31, 2018: Rejuvenation Research
Marc Beltempo, Isabelle Viel-Thériault, Roseline Thibeault, Anne-Sophie Julien, Bruno Piedboeuf
BACKGROUND: Late-onset sepsis in very low birth weight (VLBW) infants is a diagnostic challenge. We aimed to evaluate the diagnostic utility of the C-Reactive protein (CRP) and the complete blood count (CBC) for late-onset sepsis in VLBW infants. METHODS: In a 5-year retrospective cohort of 416 VLBW infants born at less than 1500 g, there were 590 separate late-onset sepsis evaluations. CRP and CBC were drawn at time of initial blood culture (T0), at 16-24 h (T24) and 40-48 h (T48) after...
January 30, 2018: BMC Pediatrics
Kansuke Koyama, Shinshu Katayama, Tomohiro Muronoi, Ken Tonai, Yuya Goto, Toshitaka Koinuma, Jun Shima, Shin Nunomiya
INTRODUCTION: The pathogenesis of thrombocytopenia in patients with sepsis is not fully understood. The aims of this study were to investigate changes in thrombopoietic activity over time by using absolute immature platelet counts (AIPC) and to examine the impact of platelet production on thrombocytopenia and mortality in patients with sepsis. METHODS: This retrospective observational study included adult patients with sepsis admitted to the intensive care unit at a university hospital...
2018: PloS One
Giovanni Nassa, Giorgio Giurato, Giovanni Cimmino, Francesca Rizzo, Maria Ravo, Annamaria Salvati, Tuula A Nyman, Yafeng Zhu, Mattias Vesterlund, Janne Lehtiö, Paolo Golino, Alessandro Weisz, Roberta Tarallo
Platelet activation triggers thrombus formation in physiological and pathological conditions, such as acute coronary syndromes. Current therapies still fail to prevent thrombotic events in numerous patients, indicating that the mechanisms modulating platelet response during activation need to be clarified. The evidence that platelets are capable of de novo protein synthesis in response to stimuli raised the issue of how megakaryocyte-derived mRNAs are regulated in these anucleate cell fragments. Proteogenomics was applied here to investigate this phenomeon in platelets activated in vitro with Collagen or Thrombin Receptor Activating Peptide...
January 11, 2018: Scientific Reports
Rosa Di Liddo, Thomas Bertalot, Alessio Borean, Ivan Pirola, Alberto Argentoni, Sandra Schrenk, Carola Cenzi, Stefano Capelli, Maria Teresa Conconi, Pier Paolo Parnigotto
The wound healing is a complex process wherein inflammation, proliferation and regeneration evolve according to a spatio-temporal pattern from the activation of coagulation cascade to the formation of a plug clot including fibrin matrix, blood-borne cells and cytokines/growth factors. Creating environments conducive to tissue repair, the haemoderivatives are commonly proposed for the treatment of hard-to-heal wounds. Here, we explored in vitro the intrinsic regenerative potentialities of a leucocyte- and platelet-rich fibrin product, known as CPL-MB, defining the stemness grade of cells sprouting from the haemoderivative...
January 5, 2018: Journal of Cellular and Molecular Medicine
A L Frelinger, R F Grace, A J Gerrits, S L Carmichael, E E Forde, A D Michelson
BACKGROUND:  Treatment decisions for patients with immune thrombocytopenia (ITP) are difficult because patients with similarly low platelet counts differ in their bleeding tendency. We recently reported that platelet function tests, independent of platelet count, are associated with concurrent bleeding severity, suggesting that these tests may be useful indicators of future bleeding in ITP. OBJECTIVES:  To test this hypothesis, we evaluated the consistency of these platelet function tests over time and their association with subsequent bleeding severity...
January 2018: Thrombosis and Haemostasis
Benjamin J Samelson-Jones, Paula M Kramer, Michael Chicka, William T Gunning Iii, Michele P Lambert
MYH9-related disease is a rare cause of thrombocytopenia. We report an infant girl who presented with severe thrombocytopenia at birth and was initially diagnosed with and treated for neonatal alloimmune thrombocytopenia. However, persistent thrombocytopenia led to the suspicion of congenital thrombocytopenia and subsequent identification of a novel variant in MYH9 (E1421K). In silico analysis strongly predicts that this is a disruptive substitution. Immunofluorescent analysis of neutrophils demonstrates abnormal aggregates of MYH9 protein...
December 29, 2017: Pediatric Blood & Cancer
Yiyan Wang, Xiaoheng Li, Fei Ge, Kaiming Yuan, Zhijian Su, Guimin Wang, Qingquan Lian, Ren-Shan Ge
Platelet-derived growth factor (PDGF) is one family of growth factors that regulate cell growth and differentiation. Rat Leydig cells express PDGF-β receptor (PDGFRB) during pubertal development. However, the mechanism of PDGF in the regulation of Leydig cell development is unclear. In the present study, rat immature Leydig cells were isolated from the testes of 35-day-old Sprague-Dawley rats and treated with 1 and 10 ng/mL of PDGF-BB. After 24 h of treatment, these cells were harvested for genomics profiling and the medium steroids were measured...
January 2018: Journal of Molecular Endocrinology
Albert N Huho, Niveen Issaq, Ionela Iacobas, Tarek M Elghetany, Dolores López-Terrada, Kevin E Fisher, Jyotinder N Punia
Pediatric chronic myelogenous leukemia is uncommon. We report a pediatric patient with chronic myelogenous leukemia presenting with a normal white blood cell count and no circulating immature myeloid cells. The patient presented with extreme thrombocytosis (platelet count range: 2175-3064 × 109 /L) noted incidentally. No splenomegaly was found. Examination of the bone marrow aspirate revealed normal cellularity and normal myeloid: erythroid ratio with marked megakaryocytic hyperplasia. Molecular studies on the bone marrow aspirate detected both the major BCR/ABL1 p210 fusion transcript (9280 copies; p210/ ABL1 ratio: 38...
January 2018: Pediatric and Developmental Pathology
Liv Vernstrøm, Kristian Løkke Funck, Erik Lerkevang Grove, Esben Laugesen, Jonathan Mathias Baier, Anne-Mette Hvas, Per Løgstrup Poulsen
INTRODUCTION: The antiplatelet effect of low-dose aspirin in patients with type 2 diabetes (T2DM) without cardiovascular disease (CVD) has not been thoroughly explored. We investigated if platelet aggregation increased during the standard 24-hour aspirin dosing interval in patients with T2DM compared to non-diabetic controls. Furthermore, we evaluated baseline platelet aggregation, the acute effects of aspirin on platelet aggregation and platelet turnover. MATERIALS AND METHODS: We included 21 patients with T2DM and 21 age and sex-matched controls...
January 2018: Thrombosis Research
Ulrike Ratsch, Thorsten Kaiser, Holger Stepan, Alexander Jank
BACKGROUND: Bone marrow function in pregnancy is influencing blood cell concentration of platelets. The steady state of consumption, recovery and production of platelets is essential for coagulation and bleeding prevention. Reticulated platelets are an intermediate form of thrombocytes during thrombopoiesis representing platelet production. The immature platelet fraction (IPF) represents these platelets as percentage of all thrombocytes. Until now, there is little knowledge on IPF during pregnancy...
October 2017: Pregnancy Hypertension
T Y He, C R Li, Y Xia, F F Liang, Y Luo, J Yang
Objective: To investigate the clinical features and genetic characteristics of cases with Ras-associated autoimmune leukoproliferative disorder(RALD). Method: Characteristics of clinical data and gene mutation of the first two cases in China with RALD were retrospectively analyzed. The related literature was searched by using search terms "NRAS" , "KRAS" or "RALD" . Result: Case1, a seven-year-seven-month old girl, was admitted due to "thrombocytopenia and splenomegaly for three years" . Palpation showed enlargement of submandibular lymph nodes and hepatosplenomegaly...
November 2, 2017: Zhonghua Er Ke za Zhi. Chinese Journal of Pediatrics
Jonathan Schupp, Franziska K Krebs, Niklas Zimmer, Emily Trzeciak, Detlef Schuppan, Andrea Tuettenberg
Myeloid cells are the most abundant cells in the tumor microenvironment (TME). The tumor recruits and modulates endogenous myeloid cells to tumor-associated macrophages (TAM), dendritic cells (DC), myeloid-derived suppressor cells (MDSC) and neutrophils (TAN), to sustain an immunosuppressive environment. Pathologically overexpressed mediators produced by cancer cells like granulocyte-macrophage colony-stimulating- and vascular endothelial growth factor induce myelopoiesis in the bone marrow. Excess of myeloid cells in the blood, periphery and tumor has been associated with tumor burden...
November 2, 2017: Cellular Immunology
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