Read by QxMD icon Read

Immature platelet

Peter Rubak, Steen D Kristensen, Anne-Mette Hvas
Immature platelets may contain more platelet enzymes such as cyclooxygenase (COX)-1 and COX-2 than mature platelets. Patients with immune thrombocytopenia (ITP) have a higher fraction of immature platelets and can therefore be utilized as a biological model for investigating COX-1 and COX-2 platelet expression. The aims were to develop flow cytometric assays for platelet COX-1 and COX-2 and to investigate the COX-1 and COX-2 platelet expression, platelet turnover, and platelet glycoproteins in ITP patients (n = 10) compared with healthy individuals (n = 30)...
October 7, 2016: Platelets
Eva Szunyogova, Haiyan Zhou, Gillian K Maxwell, Rachael A Powis, Muntoni Francesco, Thomas H Gillingwater, Simon H Parson
Spinal Muscular Atrophy (SMA) is caused by mutation or deletion of the survival motor neuron 1 (SMN1) gene. Decreased levels of, cell-ubiquitous, SMN protein is associated with a range of systemic pathologies reported in severe patients. Despite high levels of SMN protein in normal liver, there is no comprehensive study of liver pathology in SMA. We describe failed liver development in response to reduced SMN levels, in a mouse model of severe SMA. The SMA liver is dark red, small and has: iron deposition; immature sinusoids congested with blood; persistent erythropoietic elements and increased immature red blood cells; increased and persistent megakaryocytes which release high levels of platelets found as clot-like accumulations in the heart...
October 4, 2016: Scientific Reports
Eli I Lev
No abstract text is available yet for this article.
October 4, 2016: Circulation
Sidra Asad Ali, Muhammad Shariq Shaikh
No abstract text is available yet for this article.
September 2016: Journal of the College of Physicians and Surgeons—Pakistan: JCPSP
Wasiluk Alicja, Polewko Agnieszka, Laudanski Piotr, Redzko Slawomir, Kicel-Wesolowska Barbara, Dabrowska Milena, Milewski Robert
BACKGROUND: The current study objective was to compare blood platelet indices in late preterm newborns (LPN) and full term newborns (FTN). MATERIALS AND METHODS: We recruited 58 LPN and 71 FTN. Platelet indices were estimated in blood samples collected from the umbilical artery. RESULTS: LPN demonstrated a decreased count of blood platelets (249 × 10³/μL) as compared to FTN (295 × 10³/μL), p < 0.001. Platelet hematocrit (PCT) also showed substantial differences in both groups (LPN = 0...
October 6, 2016: Journal of Maternal-fetal & Neonatal Medicine
Dai Maruyama, Hirokazu Nagai, Noriko Fukuhara, Toshiyuki Kitano, Takayuki Ishikawa, Hirohiko Shibayama, Ilseung Choi, Kiyohiko Hatake, Toshiki Uchida, Momoko Nishikori, Tomohiro Kinoshita, Yoshihiro Matsuno, Tomoaki Nishikawa, Satoko Takahara, Kensei Tobinai
In this multicenter, single-arm, phase II study, efficacy and safety of ibrutinib were examined in Japanese patients with relapsed or refractory mantle cell lymphoma (MCL). Patients (age ≥ 20 years) with relapsed or refractory MCL who had progressed after receiving at least one prior treatment regimen, were enrolled. Patients were treated with oral ibrutinib (560 mg/once daily; 28-day cycle) until disease progression (or relapse), unacceptable toxicity, or study end. Primary endpoint was overall response rate (ORR)...
September 12, 2016: Cancer Science
Saeed Moradi, Ali Talati, Maryam Forghani, Amir Hossein Jafarian, Mandana Naseri, Shiva Shojaeian
OBJECTIVE: Pulp regeneration within the root canal of necrotic teeth is considered an ideal treatment to allow for continued root development and recover teeth vitality. This study aims to evaluate the inductive effect of platelet-rich plasma (PRP) on expression of angiogenesis factors and pulpal revascularization of immature necrotic teeth. MATERIALS AND METHODS: In this experimental animal study, we randomly divided 28 immature premolars from two mixed breed dogs into four groups, two experimental, negative and a positive control...
2016: Cell Journal
Sabrina Buoro, Tommaso Mecca, Michela Seghezzi, Barbara Manenti, Lorenzo Cerutti, Paola Dominoni, Gavino Napolitano, Stefano Resmini, Alberto Crippa, Cosimo Ottomano, Giuseppe Lippi
BACKGROUND: Different hematological analyzers have different analytical performances that are often reflected in the criteria for sample stability of the complete blood count. This study aimed to assess the stability of several hematological parameters using the XN-9000 Sysmex and BC-6800 Mindray analyzers. METHODS: The impact of storage at room temperature and 4°C was evaluated after 2, 4, 6, 8, 24, 36 and 48h using ten normal and 40 abnormal blood samples. The variation from the baseline measurement was evaluated by the Steel-Dwass-Critchlow-Fligner test and by Bland-Altman plots, using quality specifications and critical difference as the total allowable variation...
July 2016: Revista Brasileira de Hematologia e Hemoterapia
Christian A Di Buduo, Manuela Currao, Alessandro Pecci, David L Kaplan, Carlo L Balduini, Alessandra Balduini
Eltrombopag is a small non-peptide thrombopoietin mimetic that has been approved for increasing platelet count not only in immune thrombocytopenia and HCV-related thrombocytopenia, but also in aplastic anemia. Moreover, this drug is under investigation for increasing platelet count in myelodysplastic syndromes. Despite current clinical practice, the mechanisms governing Eltrombopag's impact on human hematopoiesis are largely unknown in part due to the impossibility of using traditional in vivo models. To investigate Eltrombopag's impact on megakaryocyte functions, we employed our established in vitro model for studying hematopoietic stem cell differentiation combined with our latest 3D silk-based bone marrow tissue model...
August 11, 2016: Haematologica
Carlos Stambolsky, Soledad Rodríguez-Benítez, José Luis Gutiérrez-Pérez, Daniel Torres-Lagares, Jenifer Martín-González, Juan José Segura-Egea
INTRODUCTION: This study evaluates histologically the efficacy of 4 revascularization protocols in necrotic-infected immature dog teeth with apical periodontitis (AP). METHODS: Forty double-rooted immature premolar teeth from 4 female Beagle dogs aged 5 months were used. Four teeth were left untouched as negative controls; the other 36 teeth were infected to develop pulp necrosis and AP. Four teeth were left untreated and assigned to the positive control group. The last 28 teeth were randomly assigned into four experimental groups of 8 teeth, each one treated with a different treatment protocol: A1, sodium hypochlorite (SH)+blood clot (BC); A2, SH+platelet-rich plasma (PRP); B1, SH+modified tri-antibiotic paste (mTAP)+BC; B2, SH+mTAP+PRP...
November 2016: Archives of Oral Biology
Christian Stratz, Thomas Nührenberg, Michael Amann, Marco Cederqvist, Pascal Kleiner, Christian M Valina, Dietmar Trenk, Franz-Josef Neumann, Willibald Hochholzer
Reticulated platelets are associated with impaired antiplatelet response to thienopyridines. It is uncertain whether this interaction is caused by a decreased drug exposure due to high platelet turnover reflected by elevated levels of reticulated platelets or by intrinsic properties of reticulated platelets. This study sought to investigate if the impact of reticulated platelets on early antiplatelet response to thienopyridines is mainly caused by platelet turnover as previously suggested. Elective patients undergoing coronary intervention were randomised to loading with clopidogrel 600 mg or prasugrel 60 mg (n=200)...
August 4, 2016: Thrombosis and Haemostasis
Albert Njoroge Huho, Niveen Issaq, Ionela Iacobas, M Tarek Elghetany, Dolores Lopez-Terrada, Kevin Fisher, Jyotinder Nain Punia
Pediatric chronic myelogenous leukemia (CML) is uncommon. We report a pediatric patient with CML presenting with a normal white blood cell count and no circulating immature myeloid cells. The patient presented with extreme thrombocytosis (platelet count range: 2,175-3,064 x 109/L) noted incidentally. No splenomegaly was found. Examination of the bone marrow aspirate (BMA) revealed normal cellularity and normal myeloid: erythroid ratio with marked megakaryocytic hyperplasia. Molecular studies on the BMA detected both the major BCR/ABL1p210 fusion transcript (9,280 copies; p210/ABL1 ratio: 38...
July 25, 2016: Pediatric and Developmental Pathology
Wen-Jun Liu, Jing Bai, Qu-Lian Guo, Zhe Huang, Hong Yang, Yong-Qi Bai
The aim of the present study was to examine and understand changes in platelet functions prior to and after the treatment of primary immune thrombocytopenia (ITP) in children. An automatic hematology analyzer and whole blood flow cytometry were used to detect immature platelet fraction (IPF), IPC and membrane glycoproteins (CD62p, PAC-1 and CD42b) in ITP children (ITP group), children with complete response after ITP treatment (ITP-CR group) and children with elective surgery (normal control group). The results showed that, levels of platelet count (PLT) and plateletcrit in the ITP group were lower alhtough the levels of mean platelet volume, platelet distribution width and platelet-large cell ratio (P-LCR) were higher than those in the normal control and ITP-CR groups...
September 2016: Molecular Medicine Reports
Gamze Topçuoğlu, Hüseyin Sinan Topçuoğlu
This report describes 3 successful single-visit regenerative endodontic therapy cases using platelet-rich plasma (PRP) and Biodentine (Septodont, Saint Maurdes Fossés, France) for 3 immature mandibular molar teeth, all with necrotic pulp, in 2 children aged 8 years and 1 aged 9 years. Three teeth were separately diagnosed as having a necrotic pulp. After preparation of the access cavity under rubber dam isolation, the necrotic pulp was removed, and each canal was irrigated with 2.5% sodium hypochlorite, sterile saline, and 17% EDTA solutions...
September 2016: Journal of Endodontics
Neal S Kleiman
No abstract text is available yet for this article.
July 19, 2016: Journal of the American College of Cardiology
Christian Stratz, Timo Bömicke, Iris Younas, Anja Kittel, Michael Amann, Christian M Valina, Thomas Nührenberg, Dietmar Trenk, Franz-Josef Neumann, Willibald Hochholzer
BACKGROUND: Previous data suggest that reticulated platelets significantly affect antiplatelet response to thienopyridines. It is unknown whether parameters describing reticulated platelets can predict antiplatelet response to thienopyridines. OBJECTIVES: The authors sought to determine the extent to which parameters describing reticulated platelets can predict antiplatelet response to thienopyridine loading compared with established predictors. METHODS: This study randomized 300 patients undergoing elective coronary stenting to loading with clopidogrel 600 mg, prasugrel 30 mg, or prasugrel 60 mg...
July 19, 2016: Journal of the American College of Cardiology
Tomohiro Muronoi, Kansuke Koyama, Shin Nunomiya, Alan Kawarai Lefor, Masahiko Wada, Toshitaka Koinuma, Jun Shima, Masayuki Suzukawa
INTRODUCTION: The diagnostic and prognostic value of immature platelet fraction (IPF) in sepsis has not been determined. This study aimed to assess whether IPF is an early predictor of platelet decline due to coagulopathy and is associated with mortality in patients with sepsis. MATERIALS AND METHODS: In total, 149 patients with a platelet count of ≥80×10(3)/μL on intensive care unit admission (101 with sepsis, 48 controls without sepsis) were prospectively evaluated...
August 2016: Thrombosis Research
Arshi Naz, Samina Naz Mukry, Mahwish Rauf Shaikh, Ali Raza Bukhari, Tahir Sultan Shamsi
BACKGROUND AND OBJECTIVE: Immune thrombocytopenic purpura (ITP) is a clinical syndrome in which a decreased number of circulating platelets (thrombocytopenia) manifests as a bleeding tendency, easy bruising (purpura) or extravasation of blood from capillaries into skin and mucous membranes (petechiae). The diagnosis of ITP can be made clinically on the basis of symptoms, we need to see if ITP can be confirmed in patients by quantification of residual RNA containing immature platelets (megakaryocytic mass) or immature platelets fraction (IPF) using automated hematology analyzers (Sysmex XE-2100)...
May 2016: Pakistan Journal of Medical Sciences Quarterly
Masayo Nakata, Takayuki Nakagomi, Mitsuyo Maeda, Akiko Nakano-Doi, Yoshihiro Momota, Tomohiro Matsuyama
Recent therapeutic advances have increased the likelihood of recanalizing the obstructed brain arteries in patients with stroke. Therefore, it is important to understand the fate of neural cells under transient ischemia/reperfusion. injury. Accumulating evidence shows that neurogenesis occurs in perivascular regions following brain injury, although the precise mechanism and origin of these newborn neurons under transient ischemia/reperfusion. injury remain unclear. Using a mouse model of transient brain ischemia/reperfusion injury, we found that neural stem cells (NSCs) develop within injured areas...
June 29, 2016: Translational Stroke Research
Min Wu, Yi-Gui Li, Da-Nian Nie, Jing Long
OBJECTIVE: To investigate the clinical significance of bone marrow morphological differences in the differential diagnosis of megaloblastic anemia (MM) and refractory anemia (R4). METHODS: A total of 60 anemia patients selected from our hospital between April 2004 and April 2015 were divided into MA group (30 cases) and RA group (30 cases) in accordance with their clinical diagnosis. Clinical manifestations, results of bone marrow morphology test, blood examination, peripheral blood smear, erythroid megaloblastic variability rate and nucleated red blood cell level in the 2 groups were compared and analyzed...
June 2016: Zhongguo Shi Yan Xue Ye Xue za Zhi
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"