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https://www.readbyqxmd.com/read/29155795/in-vivo-imaging-of-cx3cr1gfp-gfp-reporter-mice-with-spectral-domain-optical-coherence-tomography-and-scanning-laser-ophthalmoscopy
#1
Despina Kokona, Joël Jovanovic, Andreas Ebneter, Martin S Zinkernagel
Spectral domain optical coherence tomography (SD-OCT) and scanning laser ophthalmoscopy (SLO) are extensively used in experimental ophthalmology. In the present protocol, mice expressing green fluorescent protein (gfp) under the promoter of Cx3cr1 (BALB/c-Cx3cr1(gfp/gfp)) were used to image microglia cells in vivo in the retina. Microglia are resident macrophages of the retina and have been implicated in several retinal diseases(1)(,)(2)(,)(3)(,)(4)(,)(5)(,)(6). This protocol provides a detailed approach for generation of retinal B-scans, with SD-OCT, and imaging of microglia cell distribution in Cx3cr1(gfp/gfp) mice with SLO in vivo, using an ophthalmic imaging platform system...
November 11, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29148032/secondary-glaucoma-in-uveitis-comparison-of-the-optic-nerve-head-morphology-among-a-nonmydriatic-fundus-camera-hrt-and-sd-oct
#2
Milena Pahlitzsch, Matthias K J Klamann, Saskia Jacob, Carl Erb, Sibylle Winterhalter, Necip Torun, Anna-Karina B Maier, Eckart Bertelmann
PURPOSE: To assess the correlation between the disc damage likelihood scale (DDLS) objectively measured by a nonmydriatic fundus camera, confocal laser scanning ophthalmoscopy (HRT3), and spectral-domain optical coherence tomography (SD-OCT) in uveitic glaucoma. METHODS: A total of 59 patients with uveitic glaucoma (21 female, 38 male; mean age 56.8 ± 18.7 years) were included in this prospective cross-sectional study. All patients were measured by the Kowa Nonmyd WX 3D camera (2D/3D nonmydriatic retinal camera, Kowa Company), the HRT3 (Heidelberg Engineering), and SD-OCT (Carl Zeiss Meditec) by one examiner on the same day...
November 4, 2017: European Journal of Ophthalmology
https://www.readbyqxmd.com/read/29148030/incidence-and-quantitative-analysis-of-dissociated-optic-nerve-fiber-layer-appearance-real-loss-of-retinal-nerve-fiber-layer
#3
Yoon J Kim, Kyoung S Lee, Soo G Joe, June-Gone Kim
PURPOSE: To assess the association between appearance of dissociated optic nerve fiber layer (DONFL) after internal limiting membrane (ILM) peeling and changes in the macular nerve fiber layer (NFL) area by spectral-domain optical coherence tomography (SD-OCT). METHODS: This study included 132 consecutive patients who had successfully undergone vitrectomy with ILM peeling for idiopathic epiretinal membrane or macular holes and had been followed up for ≥6 months...
November 4, 2017: European Journal of Ophthalmology
https://www.readbyqxmd.com/read/29138978/-morphology-of-the-vitreoretinal-interface-in-fellow-eyes-of-patients-with-full-thickness-macular-holes
#4
J E Klaas, S Burzer, S Abraham, N Feucht, C P Lohmann, M Maier
PURPOSE: We performed a retrospective, observational clinical study to evaluate the vitreoretinal interface (VRI) in fellow eyes of patients with full thickness macular holes (FTMH) based on spectral domain optical coherence tomography (SD-OCT) examinations. METHODS: The VRI in fellow eyes of 38 patients with idiopathic FTMH, 6 of which had concomitant vitreomacular traction (VMT) and the VRI of 32 patients with FTMH with complete resolution of VMT were examined by SD-OCT for the presence of vitreomacular adhesion (VMA), VMT and the formation of FTMH, lamellar macular holes (LMH), macular pseudoholes (MPH) or epiretinal membranes (ERM)...
November 14, 2017: Der Ophthalmologe: Zeitschrift der Deutschen Ophthalmologischen Gesellschaft
https://www.readbyqxmd.com/read/29133640/evaluation-of-spectral-domain-optical-coherence-tomography-parameters-in-ocular-hypertension-preperimetric-and-early-glaucoma
#5
Tuğba Aydogan, BetÜl İlkay Sezgin Akçay, Esra Kardeş, Ahmet Ergin
PURPOSE: The objective of this study is to evaluate the diagnostic ability of retinal nerve fiber layer (RNFL), macular, optic nerve head (ONH) parameters in healthy subjects, ocular hypertension (OHT), preperimetric glaucoma (PPG), and early glaucoma (EG) patients, to reveal factors affecting the diagnostic ability of spectral domain-optical coherence tomography (SD-OCT) parameters and risk factors for glaucoma. METHODS: Three hundred and twenty-six eyes (89 healthy, 77 OHT, 94 PPG, and 66 EG eyes) were analyzed...
November 2017: Indian Journal of Ophthalmology
https://www.readbyqxmd.com/read/29131901/comparison-of-foveal-macular-and-peripapillary-intraretinal-thicknesses-between-autism-spectrum-disorder-and-neurotypical-subjects
#6
José Javier García-Medina, María García-Piñero, Mónica Del-Río-Vellosillo, Jesarán Fares-Valdivia, Ana Belén Ragel-Hernández, Salvador Martínez-Saura, María Dolores Cárcel-López, Vicente Zanon-Moreno, María Dolores Pinazo-Duran, María Paz Villegas-Pérez
Purpose: To compare thicknesses of intraretinal layers segmented by spectral-domain optical coherence tomography (SD-OCT) between autism spectrum disorder (ASD) and neurotypical (NT) individuals. Methods: We performed 2 scans on 108 eyes from 54 participants (27 high-functioning ASD and 27 age- and sex-matched NT subjects): macular fast volume and peripapillary retinal nerve fiber layer (pRNFL). Macula was automatically segmented. The mean foveal and macular thickness of nine different layers and the thickness of nine pRNFL sectors were considered...
November 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29126757/quantifying-the-rate-of-ellipsoid-zone-loss-in-stargardt-disease
#7
Cindy X Cai, Jacob G Light, James T Handa
PURPOSE: To determine a reliable method of using the ellipsoid zone (EZ) on optical coherence tomography (OCT) to track disease progression in Stardgardt disease (STGD). DESIGN: retrospective reliability study METHODS: STGD patients with genetically confirmed ABCA4 gene mutations seen at the Wilmer Eye Institute with follow-up visits separated by at least 12 months were identified. Spectral domain optical coherence tomography (SD-OCT) macula volume scans centered at the fovea and fundus autofluorescence (FAF) images were obtained...
November 7, 2017: American Journal of Ophthalmology
https://www.readbyqxmd.com/read/29125012/optical-assessment-of-the-in-vivo-tympanic-membrane-status-using-a-handheld-optical-coherence-tomography-based-otoscope
#8
Kibeom Park, Nam Hyun Cho, Mansik Jeon, Sang Heun Lee, Jeong Hun Jang, Stephen A Boppart, Woonggyu Jung, Jeehyun Kim
OBJECTIVE: Conventional otoscopes and oto-endoscopes, which are used to examine the tympanic membrane (TM), do not provide tomographic information. Optical coherence tomography (OCT) non-invasively reveals the depth-resolved internal microstructure of the TM with very high spatial resolution. We designed this study to examine the TMs with middle ear diseases using a handheld otoscope employing 860 nm spectral domain (SD)-OCT, combined with video camera and to demonstrate the clinical applicability of this system...
November 10, 2017: Acta Oto-laryngologica
https://www.readbyqxmd.com/read/29121358/optical-coherence-tomography-findings-in-endogenous-fungal-chorioretinitis-retinitis-and-endophthalmitis
#9
John D Stephens, Murtaza K Adam, Bohzo Todorich, Lisa J Faia, Sunir Garg, James P Dunn, Sonia Mehta
BACKGROUND AND OBJECTIVE: To describe spectral-domain optical coherence tomography (SD-OCT) findings in eyes with endogenous fungal chorioretinitis and endophthalmitis. PATIENTS AND METHODS: Retrospective, observational case series of subjects at Wills Eye Hospital and William Beaumont Hospital were identified by screening OCT billing data and cross-referencing with patient charts. Clinical and imaging data were collected for each patient and reviewed. RESULTS: Twelve eyes of seven consecutive patients were identified, demonstrating two patterns of posterior ocular involvement: chorioretinal infiltration and superficial retinal/retinal vascular infiltration without choroidal involvement...
November 1, 2017: Ophthalmic Surgery, Lasers & Imaging Retina
https://www.readbyqxmd.com/read/29120248/the-evaluation-of-anatomic-and-functional-changes-in-unilateral-moderate-amblyopic-eyes-using-optical-coherence-tomography-and-pupil-cycle-time
#10
Tuncay Kusbeci, Omer Karti, Eyyup Karahan, Mustafa Oguztoreli
PURPOSE: To investigate whether macular anatomic structure and afferent visual system function differ among amblyopic eyes, non-amblyopic fellow eyes, and controls, using spectral-domain optical coherence tomography (SD-OCT), and pupil cycle time (PCT). METHODS: This observational, cross-sectional study included 30 patients with unilateral amblyopia and 30 healthy subjects. Optical coherence tomography (OCT) and pupil cycle time (PCT) were used to evaluate patients with unilateral amblyopia and were compared with their non-amblyopic fellow eyes and age- and gender-matched healthy eyes (30 participants)...
November 9, 2017: Current Eye Research
https://www.readbyqxmd.com/read/29119022/bruch-s-membrane-opening-minimum-rim-width-measurement-with-sd-oct-a-method-to-correct-for-the-opening-size-of-bruch-s-membrane
#11
Robert Kromer, Martin Stephan Spitzer
A precise evaluation of the retinal nerve fiber layer thickness (RNFLT) is key for diagnosing and monitoring glaucoma. The Bruch's membrane opening minimum rim width (BMO-MRW) has been proposed as a reproducible assessment of the optic nerve. The BMO-MRW measures the minimum distance from the BMO to the internal limiting membrane. We propose an approach to correct the BMO-MRW using the BMO size for increased accuracy in interindividual comparisons in future studies. Eighty-one healthy patients received SPECTRALIS spectral domain optical coherence tomography measurements for the peripapillary RNFLT and BMO-MRW...
2017: Journal of Ophthalmology
https://www.readbyqxmd.com/read/29118497/comparing-ganglion-cell-inner-plexiform-layer-thickness-with-focal-and-global-responses-on-multifocal-electroretinogram-in-glaucoma
#12
Aparna Rao, Rohit V Chandrashekhar, Debananda Padhy, Sujoy Mukherjee, Gopinath Das, Sarada Sarangi
BACKGROUND: The aim of this study was to evaluate responses on multifocal electroretinogram (mfERG) with ganglion cell-inner plexiform layer (GCIPL) thickness on cirrus spectral-domain optical coherence tomography (SD-OCT) in glaucoma. METHODS: All diagnosed glaucoma patients attending glaucoma services at our institute from November 2012 to April 2013 were screened for this observational hospital-based study. Controls included patients attending our outpatient services for general eye checkup...
September 2017: Oman Journal of Ophthalmology
https://www.readbyqxmd.com/read/29115893/the-impact-of-epiretinal-membrane-in-neovascular-age-related-macular-degeneration-treatment-a-spectral-domain-optical-coherence-tomography-study
#13
Irini Chatziralli, Panagiotis Stavrakas, George Theodossiadis, Konstantinos Ananikas, Eleni Dimitriou, Panagiotis Theodossiadis
PURPOSE: The purpose of this prospective study was to evaluate the impact of epiretinal membrane (ERM) on anatomical and functional results in patients with wet age-related macular degeneration (AMD) treated with intravitreal anti-vascular endothelial growth (anti-VEGF) injections. METHODS: Participants in the study were 48 patients with either wet AMD alone (AMD group, n = 27) or AMD and ERM (AMD/ERM group, n = 21). All patients received intravitreal anti-VEGF injections (three monthly injections and PRN thereafter) and were followed up for at least 12 months...
November 8, 2017: Seminars in Ophthalmology
https://www.readbyqxmd.com/read/29111873/longitudinal-optical-coherence-tomography-study-of-optic-atrophy-in-secondary-progressive-multiple-sclerosis-results-from-a-clinical-trial-cohort
#14
Kimberly M Winges, Charles F Murchison, Dennis N Bourdette, Rebecca I Spain
BACKGROUND: Limited prospective information exists regarding spectral-domain optical coherence tomography (SD-OCT) in secondary progressive multiple sclerosis (SPMS). OBJECTIVE: Document cross-sectional and longitudinal retinal nerve fiber layer (RNFL) and macular ganglion cell plus inner plexiform layer (GCIPL) features of an SPMS clinical trial cohort. METHODS: Prospective, observational study using a 2-year randomized placebo-controlled SPMS trial cohort with yearly SD-OCT testing...
November 1, 2017: Multiple Sclerosis: Clinical and Laboratory Research
https://www.readbyqxmd.com/read/29111586/an-unusual-case-of-feline-acute-corneal-hydrops-atypical-disease-presentation-and-possible-in%C3%A2-vivo-detection-of-descemet-s-membrane-detachment-in-the-cat-s-unaffected-eye
#15
Brittany N Schlesener, Erin M Scott, Lucien V Vallone
A 1-year-old, female spayed, domestic shorthair cat presented for blepharospasm of the right eye. Slit-lamp biomicroscopic examination showed focal corneal ulceration and presumptive keratomalacia of the right eye. Examination of the left eye was normal apart from a focal endothelial opacity. Within the first 24 h of medical management, the right eye developed marked corneal edema and globular anterior protrusion of the corneal surface consistent with feline acute corneal hydrops (FACH). Surgical management consisted of a bridge conjunctival graft, nictitating membrane flap, and temporary tarsorrhaphy...
November 7, 2017: Veterinary Ophthalmology
https://www.readbyqxmd.com/read/29110446/dense-concentric-circle-scanning-protocol-for-measuring-pulsatile-retinal-blood-flow-in-rats-with-doppler-optical-coherence-tomography
#16
Bingyao Tan, Zohreh Hosseinaee, Kostadinka Bizheva
The variability in the spatial orientation of retinal blood vessels near the optic nerve head (ONH) results in imprecision of the measured Doppler angle and therefore the pulsatile blood flow (BF), when those parameters are evaluated using Doppler OCT imaging protocols based on dual-concentric circular scans. Here, we utilized a dense concentric circle scanning protocol and evaluated its precision for measuring pulsatile retinal BF in rats for different numbers of the circular scans. An spectral domain optical coherence tomography (SD-OCT) system operating in the 1060-nm spectral range with image acquisition rate of 47,000 A-scans/s was used to acquire concentric circular scans centered at the rat's ONH, with diameters ranging from 0...
November 2017: Journal of Biomedical Optics
https://www.readbyqxmd.com/read/29110126/-lamellar-macular-holes-with-hyporeflective-epiretinal-proliferation-oct-diagnostics-and-clinical-course
#17
REVIEW
D Vogt, F Bottoni, S G Priglinger, R G Schumann
BACKGROUND: High-resolution spectral domain optical coherence tomography (SD-OCT) is the standard examination for assessment of lamellar macular holes (LMH). According to the current SD-OCT classification of LMHs, they are characterized by (1) an irregular foveal contour, (2) a defect in the inner fovea, and (3) a separation of inner retinal layers from outer retinal layers of the fovea leading to an intraretinal splitting with loss of retinal tissue. OBJECTIVE: The article aims to give an overview on the current knowledge of retinal imaging in LMH diagnostics and clinical course of disease...
November 6, 2017: Der Ophthalmologe: Zeitschrift der Deutschen Ophthalmologischen Gesellschaft
https://www.readbyqxmd.com/read/29109902/assessment-of-spectral-domain-optical-coherence-tomography-findings-in-three-cases-of-x-linked-juvenile-retinoschisis-in-the-same-family
#18
Sibel Doğuizi, Mehmet Ali Şekeroğlu, Salih Çolak, Mustafa Alpaslan Anayol, Pelin Yılmazbaş
X-linked juvenile retinoschisis (XLRS) is an X-linked hereditary retinal dystrophy characterized by splitting of the neurosensory retina. On fundus examination, the macula often has a spoke wheel appearance with foveal cystic lesions, and separation of the retinal layers is typical on spectral-domain optical coherence tomography (SD-OCT). Patients with XLRS can exhibit different clinical courses, stages, and SD-OCT findings, even among members of the same family. SD-OCT is an important imaging method that allows us to achieve more detailed information about XLRS...
October 2017: Turkish Journal of Ophthalmology
https://www.readbyqxmd.com/read/29109897/optic-coherence-angiography-findings-in-type-2-macular-telangiectasia
#19
Hilal Nalcı, Figen Şermet, Sibel Demirel, Emin Özmert
Objectives: To evaluate the vascular changes of idiopathic macular telangiectasia type 2 (MacTel 2) patients with optical coherence tomography angiography (OCTA) and correlate these changes with the findings of spectral domain optical coherence tomography (SD-OCT). Materials and Methods: Simultaneous SD-OCT and OCTA images of 10 eyes of 6 patients who were diagnosed as MacTel 2 in Ankara University Faculty of Medicine, Department of Ophthalmology were obtained and graded according to the OCTA grading system for MacTel 2...
October 2017: Turkish Journal of Ophthalmology
https://www.readbyqxmd.com/read/29108397/internal-retinal-layer-thickness-and-macular-migration-after-internal-limiting-membrane-peeling-in-macular-hole-surgery
#20
Mun Y Faria, Nuno P Ferreira, Sofia Mano, Diana M Cristóvao, David C Sousa, Manuel E Monteiro-Grillo
PURPOSE: To provide a spectral-domain optical coherence tomography (SD-OCT)-based analysis of retinal layers thickness and nasal displacement of closed macular hole after internal limiting membrane peeling in macular hole surgery. METHODS: In this nonrandomized prospective interventional study, 36 eyes of 32 patients were subjected to pars plana vitrectomy and 3.5 mm diameter internal limiting membrane (ILM) peeling for idiopathic macular hole (IMH). Nasal and temporal internal retinal layer thickness were assessed with SD-OCT...
October 16, 2017: European Journal of Ophthalmology
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