Read by QxMD icon Read

Artur Lichtenberg

Jan-Philipp Minol, Payam Akhyari, Udo Boeken, Alexander Albert, Philipp Rellecke, Vanessa Dimitrova, Stephan Urs Sixt, Hiroyuki Kamiya, Artur Lichtenberg
Background: Cardiac redo surgery, especially after a full sternotomy, is considered a high-risk procedure. Minimally invasive mitral valve surgery (MIMVS) is a potential therapeutic approach. However, current developments in interventional cardiology necessitate additional discussion regarding the therapy of choice in high-risk patients. In this context, it is necessary to clarify the perioperative and postoperative risks induced by the factor previous sternotomy in the setting of MIMVS...
2018: Frontiers in Surgery
Jörn Hülsmann, Hug Aubin, Fabian Oberle, Nicolas Schuetterle, Shahbaz T Bandesha, Makoto Iijima, Artur Lichtenberg, Payam Akhyari
Whole-organ engineering - based on the functional repopulation of acellular whole-organ scaffolds derived from perfusion-based in toto decellularization of the specific organ system - is one of the most promising fields in tissue engineering. However, to date, we still have hardly any insights into the process of perfusion-based scaffold generation itself, with human-scale scaffolds usually obtained by adoption of small animal decellularization models, although those organs are of decreased biomass and potentially different biological characteristics...
February 21, 2018: Biomedical Materials
Diyar Saeed, Bujar Maxhera, Najla Sadat, Georgi Petrov, Alexander Albert, Artur Lichtenberg
We aimed to investigate the prevalence of gastrointestinal bleeding (GIB) events in patients supported with HeartWare ventricular assist device (VAD) and activated lavare cycle. Thirty-two GIB events were documented in 22 patients (21%) after median support duration of 22 days (IQR: 11-157 days). There were 13 patients with early episodes of GIB. Meanwhile, 17 GIB events were documented in 9 patients after a median support duration of 174 days (IQR 25-736 days) (late bleeders), accounting for 0.18 events per patient's years...
January 2018: ASAIO Journal: a Peer-reviewed Journal of the American Society for Artificial Internal Organs
Sebastian Temme, Daniela Friebe, Timo Schmidt, Gereon Poschmann, Julia Hesse, Bodo Steckel, Kai Stühler, Meik Kunz, Thomas Dandekar, Zhaoping Ding, Payam Akhyari, Artur Lichtenberg, Jürgen Schrader
Epicardium-derived cells (EPDC) and atrial stromal cells (ASC) display cardio-regenerative potential, but the molecular details are still unexplored. Signals which induce activation, migration and differentiation of these cells are largely unknown. Here we have isolated rat ventricular EPDC and rat/human ASC and performed genetic and proteomic profiling. EPDC and ASC expressed epicardial/mesenchymal markers (WT-1, Tbx18, CD73, CD90, CD44, CD105), cardiac markers (Gata4, Tbx5, troponin T) and also contained phosphocreatine...
December 2017: Stem Cell Research
Payam Akhyari, Fabian Oberle, Joern Huelsmann, Hans Heid, Stefan Lehr, Andreas Barbian, Sentaro Nakanishi, Hug Aubin, Alexander Jenke, Artur Lichtenberg
Whole-organ engineering is an innovative field of regenerative medicine with growing translational perspectives. Recent reports suggest the feasibility of decellularization and repopulation of entire human size hearts. However, little is known about the susceptibility of epicardial adipose tissue (EAT) to decellularization. Here, human size hearts of ovine donors were subjected to perfusion-based decellularization using detergent solutions. Upon basic histological evaluation and total DNA measurement myocardial regions prove largely decellularized while EAT demonstrated cellular remnants, further confirmed by transmission electron microscopy...
September 12, 2017: Tissue Engineering. Part A
Hug Aubin, George Petrov, Hannan Dalyanoglu, Maximillian Richter, Diyar Saeed, Payam Akhyari, Detlef Kindgen-Milles, Alexander Albert, Artur Lichtenberg
AIM: Mobile extracorporeal life support (ECLS) may soon be on the verge to become a fundamental part of emergency medicine. Here, we report on our four-year experience of providing advanced mechanical circulatory support for out-of-center patients within the Düsseldorf ECLS Network (DELSN). METHODS: This retrospective cohort study analyses the outcome of 160 patients with refractory circulatory failure consecutively treated with mobile veno-arterial extracorporeal membrane oxygenation (vaECMO) between July 2011 and October 2015 within the DELSN...
December 2017: Resuscitation
Yukiharu Sugimura, Anna Kathrin Schmidt, Artur Lichtenberg, Alexander Assmann, Payam Akhyari
The demand for an improvement of the biocompatibility and durability of vascular and valvular implants requires translational animal models to study the in vivo fate of cardiovascular grafts. In the present article, a review on the development and application of a microsurgical rat model of infrarenal implantation of aortic grafts and aortic valved conduits is provided. By refinement of surgical techniques and inclusion of hemodynamic considerations, a functional model has been created, which provides a modular platform for the in vivo assessment of biological and tissue-engineered grafts...
December 2017: Tissue Engineering. Part C, Methods
Katrin Botzet, Hannan Dalyanoglu, Ralf Schäfer, Artur Lichtenberg, Jochen D Schipke, Bernhard Korbmacher
No abstract text is available yet for this article.
August 6, 2017: Thoracic and Cardiovascular Surgeon
Stephan Sixt, Hug Aubin, Robert Kalb, Philipp Rellecke, Artur Lichtenberg, Alexander Albert
BACKGROUND: In the past, minimally invasive cardiac surgery (MICS)- coronary artery bypass graft surgery (CABG) alternatives have been introduced that dramatically reduce the invasiveness of standard operative procedures while still showing excellent clinical outcomes. However, in patients with high morbidity, reduced lung function impeding single-lung ventilation is one of the major concerns for MICS-CABG procedures, although those patients might reap the largest benefit from a procedure of reduced invasiveness...
December 2017: Annals of Thoracic Surgery
Anna K Schmidt, Diyar Saeed, Arash Mehdiani, Bozena Sowinski, Ralf Westenfeld, Payam Akhyari, Artur Lichtenberg, Udo Boeken
INTRODUCTION: In times of organ shortage, death while on the heart waiting-list still represents a major problem. As a consequence, bridging to transplant as well as the decision when to escalate therapy play a very important role. METHODS AND RESULTS: We report on two young patients with dilated cardiomyopathy and acute decompensation who were successfully bridged to heart transplantation with both left and temporary right ventricular assist devices in just 2 months...
October 27, 2017: International Journal of Artificial Organs
Arash Mehdiani, Payam Akhyari, Hiroyuki Kamiya, Joachim Ahlers, Erhard Godehardt, Alexander Albert, Udo Boeken, Artur Lichtenberg
Background The aim of the study was to assess the value of post-operative cardiac troponin T-levels, measured with a new highly sensitive assay (hs-cTnT), as a suitable parameter to predict patients' outcome after cardiac surgery. With the introduction of the new hs-cTnT assay the correlation between measured levels and the post-operative patient's outcome remains to be evaluated. Methods Patients undergoing coronary artery bypass grafting (n = 213) were included. Perioperative measurements of hs-cTnT and CK-MB were correlated to parameters of clinical outcome and further explored...
June 2017: Acta Cardiologica
Jan-Philipp Minol, Isabella Reinsch, Maximilian Luik, Anne Leferink, Mareike Barth, Alexander Assmann, Artur Lichtenberg, Payam Akhyari
Reactive oxygen species (ROS) play an important role in the process of cardiovascular degeneration. We evaluated the potential of a controlled, local induction of ROS-release by application of rose bengal (RB) and photo energy to induce atherosclerosis-like focal vascular degeneration in vivo. After injection of RB, rats fed with a pro-degenerative diet underwent focal irradiation of the abdominal aorta by a green laser (ROS group), while the controls received irradiation without RB. Aortic tissue was analyzed by histology and immunohistochemistry at 0, 2, 4, 8, 28 and 56 days (n = 5)...
2017: PloS One
Elena V Timchenko, Pavel E Timchenko, Artur Lichtenberg, Alexander Assmann, Hug Aubin, Payam Akhyari, Larisa T Volova, Svetlana V Pershutkina
We report the results of experimental studies on cardiac implants using a Raman spectroscopy method (RS). Raman spectra characteristics of leaves and walls of cardiac implants were obtained; the implants were manufactured by protocols of detergent-enzymatic technique (DET) and biological, detergent-free (BIO) decellularization, using detergents (group DET) or a detergent-free, nonproteolytic, actin-disassembling regimen (BIO). There were input optical coefficients that allowed us to carry out evaluation of the protocols of DET and BIO decellularization on the basis of the concentrations of glycosaminoglycans, proteins, amides, and DNA...
September 1, 2017: Journal of Biomedical Optics
Hug Aubin, Jörn Hülsmann, Artur Lichtenberg, Payam Akhyari
No abstract text is available yet for this article.
April 15, 2017: Acta Biomaterialia
Diyar Saeed, Alexander Assmann, Muhammad Abdeen, Alexander Albert, Bujar Maxhera, Najla Sadat, Stephan Sixt, Artur Lichtenberg
Selected patients who fail to be weaned off temporary veno-arterial extracorporeal membrane oxygenation support may be considered for long-term left ventricular assist devices.  We describe here a left ventricular assist device implantation technique in patients with prior veno-arterial extracorporeal membrane oxygenation support without the use of a cardiopulmonary bypass machine, which minimizes the intraoperative trauma and blood loss while still meeting all the goals of the standard procedure.
December 9, 2016: Multimedia Manual of Cardiothoracic Surgery: MMCTS
Alexander Assmann, Marc Struß, Franziska Schiffer, Friederike Heidelberg, Hiroshi Munakata, Elena V Timchenko, Pavel E Timchenko, Tim Kaufmann, Khon Huynh, Yukiharu Sugimura, Quentin Leidl, Antonio Pinto, Volker R Stoldt, Artur Lichtenberg, Payam Akhyari
Low immunogenicity and high repopulation capacity are crucial determinants for the functional and structural performance of acellular cardiovascular implants. The present study evaluates a detergent-free, non-proteolytic, actin-disassembling regimen (BIO) for decellularization of heart valve and vessel grafts, particularly focusing on their bio-functionality. Rat aortic conduits (rAoC; n = 89) and porcine aortic valve samples (n = 106) are decellularized using detergents (group DET) or the BIO regimen...
January 11, 2017: Journal of Tissue Engineering and Regenerative Medicine
Jörn Hülsmann, Hug Aubin, Alexander Wehrmann, Artur Lichtenberg, Payam Akhyari
Here, we investigate the impact of integrated three-dimensional (3D) left ventricular (LV) stretching on myocardial maturation in a whole-heart bioreactor setting. Therefore, decellularized rat hearts were selectively repopulated with rodent neonatal cardiomyocytes (5 · 10(6) cells per heart) and cultured over 5 days. Continuous medium perfusion was maintained through the coronary artery system in a customized whole-heart bioreactor system with or without integrated biomechanical stimulation of LV. 3D repopulation effectiveness and cellular vitality were evaluated by repetitive metabolic WST-1 assays and 3D confocal microscopy analysis through fluorescent staining, also assessing cellular organization...
May 2017: Biotechnology and Bioengineering
Antonio Pinto, Moritz Benjamin Immohr, Annika Jahn, Alexander Jenke, Udo Boeken, Artur Lichtenberg, Payam Akhyari
OBJECTIVES: Cardiac surgery with cardiopulmonary bypass (CPB) provokes ischaemia and reperfusion injury (IRI). Superoxide is a main mediator of IRI and is detoxified by superoxide dismutases (SODs). Extracellular SOD (SOD3) is the prevailing isoform in the cardiovascular system. Its mutation is associated with elevated risk for ischaemic heart disease as epidemiological and experimental studies suggest. We investigated the influence of SOD3 on IRI in the context of CPB and hypothesized a protective role for this enzyme...
December 2016: European Journal of Cardio-thoracic Surgery
Makoto Iijima, Hug Aubin, Meike Steinbrink, Franziska Schiffer, Alexander Assmann, Richard D Weisel, Yoshiro Matsui, Ren-Ke Li, Artur Lichtenberg, Payam Akhyari
No ideal small-diameter vascular graft for widespread clinical application has yet been developed and current approaches still suffer from graft failure because of thrombosis or degeneration. Decellularized vascular grafts are a promising strategy as they preserve native vessel architecture while eliminating cell-based antigens and allow for autologous recellularization. In the present study, a functional in vivo rodent aortic transplantation model was used in order to evaluate the benefit of bioactive coating of decellularized vascular grafts with vascular endothelial growth factor (VEGF) conjugated to a temperature-sensitive aliphatic polyester hydrogel (HG)...
September 30, 2016: Journal of Tissue Engineering and Regenerative Medicine
Diyar Saeed, Stephan Sixt, Alexander Albert, Artur Lichtenberg
No abstract text is available yet for this article.
November 2016: Journal of Thoracic and Cardiovascular Surgery
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"