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IL-1 AND Asthma

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https://www.readbyqxmd.com/read/28528200/sputum-transcriptomics-reveal-up-regulation-of-il-1-receptor-family-members-in-severe-asthma
#1
Christos Rossios, Stelios Pavlidis, Uruj Hoda, Chih-Hsi Kuo, Coen Wiegman, Kirsty Russell, Kai Sun, Matthew J Loza, Frederic Baribaud, Andrew L Durham, Oluwaseun Ojo, Rene Lutter, Anthony Rowe, Aruna Bansal, Charles Auffray, Ana Sousa, Julie Corfield, Ratko Djukanovic, Yike Guo, Peter J Sterk, Kian Fan Chung, Ian M Adcock
BACKGROUND: Sputum analysis in asthma is used to define airway inflammatory processes and may guide therapy. OBJECTIVE: To determine differential gene and protein expression in sputum samples from patients with severe asthma (SA) compared to mild-moderate non-smoking asthmatics (MMA). METHODS: Induced sputum was obtained from non-smoking SA (SAn), smokers/ex-smokers with SA (SAsm), MMA and healthy non-smoking controls. Differential cell counts, microarray analysis of cell pellets and SOMAscan analysis of sputum analytes was performed...
May 17, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28524369/therapeutic-roles-of-telocytes-in-ova-induced-acute-asthma-in-mice
#2
Ling Ye, Dongli Song, Meiling Jin, Xiangdong Wang
Telocytes (TCs) newly discovered as the mesenchyme-derived interstitial cells were found to have supportive effects on mesenchymal stem cells (MSCs). The present study aimed at investigating effects of TCs or TCs gathered with MSCs on experimental airway inflammation and hyper-responsiveness. The TCs were isolated from the lung tissue of the female BALB/c mice. The ovalbumin (OVA)-induced asthma model was established. TCs (1 × 10(6) /2 × 10(6) ) and/or MSCs (1 × 10(6) ) were injected through mice tail vein for consecutive three days before OVA excited the mice...
May 19, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28522569/brain-derived-neurotrophic-factor-and-airway-fibrosis-in-asthma
#3
Michelle R Freeman, Venkatachalem Sathish, Logan J Manlove, Shengyu Wang, Rodney D Britt, Michael A Thompson, Christina M Pabelick, Y S Prakash
Airway remodeling in asthma driven by inflammation involves proliferation of epithelial cells and airway smooth muscle (ASM), as well as enhanced extracellular matrix (ECM) generation and deposition, i.e. fibrosis. Accordingly, understanding pro-fibrotic mechanisms is important for developing novel therapeutic strategies in asthma. Recent studies, including our own, have suggested a role for locally-produced growth factors such as brain-derived neurotrophic factor (BDNF) in mediating and modulating inflammation effects...
May 18, 2017: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/28515358/irf5-distinguishes-severe-asthma-in-humans-and-drives-th1-phenotype-and-airway-hyperreactivity-in-mice
#4
Timothy B Oriss, Mahesh Raundhal, Christina Morse, Rachael E Huff, Sudipta Das, Rachel Hannum, Marc C Gauthier, Kathryn L Scholl, Krishnendu Chakraborty, Seyed M Nouraie, Sally E Wenzel, Prabir Ray, Anuradha Ray
Severe asthma (SA) is a significant problem both clinically and economically, given its poor response to corticosteroids (CS). We recently reported a complex type 1-dominated (IFN-γ-dominated) immune response in more than 50% of severe asthmatics despite high-dose CS treatment. Also, IFN-γ was found to be critical for increased airway hyperreactivity (AHR) in our model of SA. The transcription factor IRF5 expressed in M1 macrophages can induce a Th1/Th17 response in cocultured human T cells. Here we show markedly higher expression of IRF5 in bronchoalveolar lavage (BAL) cells of severe asthmatics as compared with that in cells from milder asthmatics or healthy controls...
May 18, 2017: JCI Insight
https://www.readbyqxmd.com/read/28509396/anti-inflammatory-antioxidant-and-immunomodulatory-effects-of-curcumin-in-ovalbumin-sensitized-rat
#5
Farzaneh Shakeri, Mohammad Hossein Boskabady
BACKGROUND: Anti-inflammatory and antioxidant properties of curcumin have been shown. In this study, anti-inflammatory, antioxidant, and immunomodulatory effects of curcumin in sensitized rat were evaluated. METHODS: Six groups of rats including control (C), ovalbumin-sensitized (as a rat model of asthma, S), S groups treated with curcumin (Cu 0.15, 0.30, and 0.60 mg/mL), and 1.25 μg/mL dexamethasone (S + D) were studied. Curcumin and dexamethasone were given in animals' drinking water during sensitization period...
May 16, 2017: BioFactors
https://www.readbyqxmd.com/read/28502165/grape-seed-proanthocyanidin-inhibits-mucin-synthesis-and-viral-replication-by-suppression-of-ap-1-and-nf-%C3%AE%C2%BAb-via-p38-mapks-jnk-signaling-pathways-in-respiratory-syncytial-virus-infected-a549-cells
#6
Jin-Woo Lee, Young Il Kim, Chang-Nim Im, Sung Wan Kim, Su Jin Kim, Seoyeon Min, Yong Hoon Joo, Sung-Vin Yim, Namhyun Chung
Airway epithelial cells are often infected by respiratory syncytial virus (RSV), one of the most common causes of asthma, bronchiolitis, chronic obstructive pulmonary disease (COPD), and pneumonia. During the infection process, excessive mucins aggravate airway inflammation. However, the mechanism underlying RSV-induced airway hyperresponsiveness and inflammation is poorly understood. Furthermore, no reliable vaccines or drugs for antiviral therapy are available. In this study, the effect of the natural compound grape seed proanthocyanidin (GSP) on RSV-infected human airway epithelial cells A549 was evaluated...
May 15, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28500314/activation-of-eosinophils-interacting-with-bronchial-epithelial-cells-by-antimicrobial-peptide-ll-37-implications-in-allergic-asthma
#7
Delong Jiao, Chun-Kwok Wong, Miranda Sin-Man Tsang, Ida Miu-Ting Chu, Dehua Liu, Jing Zhu, Man Chu, Christopher Wai-Kei Lam
The role of antimicrobial peptide LL-37 in asthma exacerbation is unclear. Microbial infection, which is the most common inducer of asthma exacerbation, is accompanied by elevated LL-37. The present study found that co-culture of eosinophils and bronchial epithelial cell line BEAS-2B significantly enhanced intercellular adhesion molecule-1 on both cells and CD18 expression on eosinophils upon LL-37 stimulation. IL-6, CXCL8 and CCL4 were substantially released in co-culture in the presence of LL-37. LL-37 triggered the activation of eosinophils interacting with BEAS-2B cells in a P2X purinoceptor 7/epidermal growth factor receptor-dependent manner...
May 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28493079/tlr2-regulates-allergic-airway-inflammation-and-autophagy-through-pi3k-akt-signaling-pathway
#8
Xuqin Jiang, Lei Fang, Huimei Wu, Xiaodong Mei, Fang He, Peishan Ding, Rongyu Liu
Toll-like receptors (TLRs) are innate pattern recognition receptors that play a critical role in allergic inflammation, yet their contribution to autophagy in asthma remains poorly defined. Here, we investigate the role of Toll-like receptor 2 (TLR2) in phosphoinositide 3-kinases/protein kinase B (PI3K/Akt) pathway-mediated autophagy in ovalbumin-induced airway inflammation in mice. Wild-type (WT) and TLR2-knockout (TLR2(-/-)) C57BL/6 mice were ovalbumin-sensitized and ovalbumin-challenged. In ovalbumin-challenged WT mice, enhanced expression of TLR2 in lung tissue, remarkable inflammatory cell infiltrates, goblet cell hyperplasia, and increased mucus production were observed...
May 10, 2017: Inflammation
https://www.readbyqxmd.com/read/28483577/formoterol-and-fluticasone-propionate-combination-improves-histone-deacetylation-and-anti-inflammatory-activities-in-bronchial-epithelial-cells-exposed-to-cigarette-smoke
#9
M Ferraro, M Gjomarkaj, L Siena, S Di Vincenzo, E Pace
BACKGROUND: The addition of long-acting beta2-agonists (LABAs) to corticosteroids improves asthma control. Cigarette smoke exposure, increasing oxidative stress, may negatively affect corticosteroid responses. The anti-inflammatory effects of formoterol (FO) and fluticasone propionate (FP) in human bronchial epithelial cells exposed to cigarette smoke extracts (CSE) are unknown. AIMS: This study explored whether FP, alone and in combination with FO, in human bronchial epithelial cells line (16-HBE) and primary bronchial epithelial cells (NHBE), counteracted some CSE-mediated effects and in particular some of the molecular mechanisms of corticosteroid resistance...
May 5, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28479600/a-crucial-role-of-the-pd-1h-coinhibitory-receptor-in-suppressing-experimental-asthma
#10
Huafeng Liu, Xin Li, Li Hu, Min Zhu, Bailin He, Liqun Luo, Lieping Chen
Programmed death one homolog (PD-1H) is a cell surface molecule of the B7/CD28 immune modulatory gene family. Although PD-1H has been shown to function as a coinhibitory receptor on T cells to limit naive T-cell activation and proliferation, its role in the regulation of the T-cell response to allergens is unknown. We report here that genetic ablation or blockade of PD-1H drastically promotes pulmonary inflammation with massive accumulation of eosinophils in a mouse model of experimental asthma, indicating a suppressive function of PD-1H in allergic inflammation...
May 8, 2017: Cellular & Molecular Immunology
https://www.readbyqxmd.com/read/28477201/isolation-and-purification-of-th9-cells-for-the-study-of-inflammatory-diseases-in-research-and-clinical-settings
#11
Patricia Keating, James X Hartmann
Th9 cells are associated with atopic and inflammatory diseases, and their increased levels and function correlate with the severity of symptoms in various inflammatory disorders including asthma, food allergy, atopic dermatitis, ulcerative colitis, and psoriatic arthritis. Thus, clinical trials are warranted to evaluate the role of Th9 cells in allergic diseases with the goal of controlling these ailments.Circulating T cells (naïve or memory CD4+ T cells) purified from human blood and expanded using anti-CD3 and anti-CD28 antibodies can be treated with appropriate cytokines in order to polarize them to the Th9 phenotype as evidenced by their production of IL-9...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28477193/transcription-factors-downstream-of-il-4-and-tgf-%C3%AE-signals-analysis-by-quantitative-pcr-western-blot-and-flow-cytometry
#12
Atsushi Sugimoto, Ryoji Kawakami, Norihisa Mikami
IL-9-producing Th9 cell is a novel Th cell subset involved in type II allergic inflammations such as asthma. Th9 cells can be induced from naïve Th cells in the presence of IL-4 and TGF-β. It is also well established that downstream signals of IL-4 and TGF-β, including STAT6, IRF4, Smad, and PU.1, directly mediate IL-9 production in Th9 cells. In this chapter we describe the methods of flow cytometry, qPCR and western blot analysis to determine the expression or activation of these transcription factors downstream of IL-4 and TGF-β...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28474843/n-glycan-in-cockroach-allergen-regulates-human-basophil-function
#13
Danh C Do, Shuang Yang, Xu Yao, Robert G Hamilton, John T Schroeder, Peisong Gao
INTRODUCTION: Cockroach allergen exposure elicits cockroach sensitization and poses an increased risk for asthma. However, the major components in cockroach allergen and the mechanisms underlying the induction of cockroach allergen-induced allergy and asthma remain largely elusive. We sought to examine the role of cockroach-associated glycan in regulating human basophil function. METHODS: N-linked glycans from naturally purified cockroach allergen Bla g 2 were characterized by MALDI-TOF mass spectrometry...
February 20, 2017: Immunity, Inflammation and Disease
https://www.readbyqxmd.com/read/28474507/il-17f-induces-il-6-via-tak1-nf%C3%AE%C2%BAb-pathway-in-airway-smooth-muscle-cells
#14
Masayuki Nakajima, Mio Kawaguchi, Kyoko Ota, Junichi Fujita, Satoshi Matsukura, Shau-Ku Huang, Yuko Morishima, Yukio Ishii, Hiroaki Satoh, Tohru Sakamoto, Nobuyuki Hizawa
INTRODUCTION: Interleukin (IL)-17F plays a critical role in the pathophysiology of asthma. However, the precise role of IL-17F in airway smooth muscle cells (ASMCs) and its regulatory mechanisms remain to be defined. Therefore, we sought to investigate the expression of IL-6 by IL-17F and the involvement of transforming growth factor β-activated kinase 1 (TAK1) and nuclear factor (NF)-κB by in ASMCs. METHODS: ASMCs were cultured in the presence or absence of IL-17F...
June 2017: Immunity, Inflammation and Disease
https://www.readbyqxmd.com/read/28471975/thymic-stromal-lymphopoietin-il-33-and-periostin-in-hospitalized-infants-with-viral-bronchiolitis
#15
María Luz García-García, Cristina Calvo, Ana Moreira, José Antonio Cañas, Francisco Pozo, Beatriz Sastre, Sergio Quevedo, Inmaculada Casas, Victoria Del Pozo
Much attention has recently been focused on thymic stromal lymphopoietin (TSLP), IL-33, and periostin in allergic disease, but less is known about their role in viral bronchiolitis.The aim of the study was to investigate whether infants exhibit enhanced nasal airway secretion of TSLP, IL-33, and periostin during natural respiratory viral bronchiolitis compared to healthy controls.In total, 213 infants < 2 years of age, hospitalized with bronchiolitis from October/2013 to April/2016 were enrolled alongside 45 healthy infants...
May 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28465252/antioxidant-and-anti-inflammatory-activities-of-the-major-phenolics-from-zygophyllum-simplex-l
#16
Hossam M Abdallah, Ahmed Esmat
ETHNOPHARMACOLOGICAL RELEVANCE: Zygophyllum simplex L. is a halophyte plant that follows Zygophyllaceae. The plant is growing in arid and semiarid regions. It has been used traditionally in Arabic region to treat gout, asthma and inflammation. AIM OF STUDY: Although ant-inflammatory activity has been reported for this plant, this study aimed to isolate and identify the major constituents of Zygophyllum simplex L., as well as assessing their antioxidant and anti-inflammatory activities in-vitro...
April 29, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28461298/differences-between-asthma-copd-overlap-syndrome-and-adult-onset-asthma
#17
Minna Tommola, Pinja Ilmarinen, Leena E Tuomisto, Lauri Lehtimäki, Jussi Haanpää, Onni Niemelä, Hannu Kankaanranta
Differences between asthma-COPD overlap syndrome (ACOS) and adult-onset asthma are poorly understood. This study aimed to evaluate these differences in a clinical cohort of patients with adult-onset asthma, as a part of the Seinäjoki Adult Asthma Study (SAAS).188 patients were diagnosed with adult-onset asthma and re-evaluated 12 years after diagnosis. They were divided into three groups based on smoking history and post bronchodilator spirometry values: 1) never- and ex-smokers with <10 smoked pack-years; 2) non-obstructive (forced expiratory volume in 1 s (FEV1)/forced vital capacity (FVC) ≥0...
May 2017: European Respiratory Journal: Official Journal of the European Society for Clinical Respiratory Physiology
https://www.readbyqxmd.com/read/28444548/post-effect-of-air-quality-improvement-on-biomarkers-for-systemic-inflammation-and-microparticles-in-asthma-patients-after-the-2008-beijing-olympic-games-a-pilot-study
#18
Jinming Gao, Xiaohua Xu, Zhekang Ying, Lei Jiang, Mianhua Zhong, Aixia Wang, Lung-Chi Chen, Bo Lu, Qinghua Sun
This study's aim was to investigate the post-effect of an air quality improvement on systemic inflammation and circulating microparticles in asthmatic patients during, and 2 months after, the Beijing Olympics 2008. We measured the levels of circulating inflammatory cytokines and microparticles in the peripheral blood from asthma patients and healthy controls during (phase 1), and 2 months after (phase 2) the Beijing 2008 Olympic Games. The concentrations of circulating cytokines (including TNFα, IL-6, IL-8, and IL-10) were still seen reduced in phase 2 when compared with those in phase 1...
April 25, 2017: Inflammation
https://www.readbyqxmd.com/read/28439933/effect-of-anti-ige-in-occupational-asthma-caused-by-exposure-to-low-molecular-weight-agents
#19
M Ollé-Monge, M J Cruz, S Gomez-Ollés, I Ojanguren, J Vanoirbeek, X Muñoz
BACKGROUND: The role of immunoglobulin (Ig)-E in occupational asthma (OA) due to low molecular weight (LMW) agents is not well established compared to classical atopic asthma. In the present study we evaluate whether anti-IgE monoclonal antibody (mAb) has an effect in a mouse model of OA, using persulfate salts. METHODS: On days 1 and 8, BALB/C mice were dermally sensitized with 5% ammonium persulfate (AP) or dimethyl sulfoxide (DMSO). On days 15, 18 and 21 animals were injected intraperitoneally with anti-IgE mAb or PBS 6 hours before challenge with AP or saline...
April 25, 2017: Allergy
https://www.readbyqxmd.com/read/28437599/human-%C3%AE-defensin-3-induces-il-8-release-and-apoptosis-in-airway-smooth-muscle-cells
#20
Weijia Wang, Xiaoyan Qu, Xiaomin Dang, Dong Shang, Liang Yang, Ying Li, Dan Xu, James G Martin, Qutayba Hamid, Jiankang Liu, Ying Chang
BACKGROUND: Human airway smooth muscle cells (ASMCs) may have a pro-inflammatory role through the release of inflammatory mediators. Increasing evidence indicates that human β-defensins (HBDs) are related to pathogenesis of asthma. OBJECTIVES: To examine the plasma level of HBD-1, -2 and -3 in asthmatic patients and the expression of their mouse orthologs in the lung tissue of a mouse model of chronic severe asthma. Further to investigate the effect of HBD-3 on the release of the pro-inflammatory cytokine IL-8 and to explore the mechanisms...
April 24, 2017: Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology
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