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https://www.readbyqxmd.com/read/28109042/expression-and-characterization-of-a-codon-optimized-blood-coagulation-factor-viii
#1
S A Shestopal, J-J Hao, E Karnaukhova, Y Liang, M V Ovanesov, M Lin, J H Kurasawa, T K Lee, J H McVey, A G Sarafanov
BACKGROUND: Production of recombinant factor VIII (FVIII) is challenging due to its low expression. It was previously shown that codon-optimization of a B domain-deleted FVIII (BDD-FVIII) cDNA resulted in increased protein expression. However, it is well-recognized that synonymous mutations may affect the protein structure and function. OBJECTIVES: To compare biochemical properties of a BDD-FVIII expressed from codon-optimized (CO) and the wild-type (WT) cDNAs. METHODS: Each variant of the BDD-FVIII was expressed in several independent CHO cell lines, generated using a lentiviral platform...
January 21, 2017: Journal of Thrombosis and Haemostasis: JTH
https://www.readbyqxmd.com/read/28108862/effects-of-chromium-methionine-supplementation-with-different-sources-of-zinc-on-growth-performance-carcass-traits-meat-quality-serum-metabolites-endocrine-parameters-and-the-antioxidant-status-in-growing-finishing-pigs
#2
Xiao Xu, Li Liu, Shen-Fei Long, Xiang-Shu Piao, Terry L Ward, Fei Ji
This experiment was conducted to evaluate the effects of chromium methionine with/without zinc sulfate or zinc amino acid complex on the growth performance, carcass traits, meat quality, serum parameters, endocrine parameters, and antioxidant status of growing-finishing pigs. A total of 180 (32.0 ± 1.7 kg body weight, BW) crossbred pigs (Duroc × Landrace × Yorkshire) were used in a completely randomized design with three dietary treatments and 10 replicates per treatment (five pens of barrows and five pens of gilts with six pigs per replicate)...
January 21, 2017: Biological Trace Element Research
https://www.readbyqxmd.com/read/28108328/distribution-of-phospholipid-based-formulations-in-the-skin-investigated-by-combined-atr-ftir-and-tape-stripping-experiments
#3
Martin Wolf, Maria Halper, Raffaela Pribyl, Dieter Baurecht, Claudia Valenta
The spatial distribution of exogenous substances in the stratum corneum (SC) could have an influence on their skin irritation potential. In this study it was possible to monitor the distribution of phospholipids with their phosphatidylcholine scaffold on porcine ear skin by combining tape stripping and in vitro ATR-FTIR spectroscopy. Significant vibrational modes in the spectra could be successfully assigned to the functional groups of the molecules. Thus it was possible to track the phospholipids without the need of their deuterated form by calculating difference spectra from the treated - untreated skin samples...
January 17, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28108094/metabolic-profiling-identification-of-metabolites-formed-in-mediterranean-mussels-mytilus-galloprovincialis-after-diclofenac-exposure
#4
Bénilde Bonnefille, Lauren Arpin-Pont, Elena Gomez, Hélène Fenet, Frédérique Courant
Despite the growing concern on the presence of pharmaceutically active compounds in the environment, few studies have been conducted on their metabolism in marine organisms. In this study, a non-targeted strategy based on the generation of chemical profiles generated by liquid chromatography combined with high resolution mass spectrometry was used to highlight metabolite production by the Mediterranean mussel (Mytilus galloprovincialis) after diclofenac exposure. This method allowed revealing the production of 13 metabolites in mussel tissues...
January 17, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28107912/maldi-ltq-orbitrap-mass-spectrometry-imaging-for-lipidomic-analysis-in-kidney-under-cisplatin-chemotherapy
#5
Estefanía Moreno-Gordaliza, Diego Esteban-Fernández, Alberto Lázaro, Blanca Humanes, Sarah Aboulmagd, Alberto Tejedor, Michael W Linscheid, M Milagros Gómez-Gómez
Imaging techniques for mapping molecular distributions in tissue sections can reveal valuable information on biomolecules involved in relevant biochemical processes. A method has been developed for comprehensive, reproducible and sensitive lipid imaging by matrix-assisted laser/desorption ionization-LTQ-Orbitrap mass spectrometry in kidney sections, showing the benefits of exact mass determination. Matrix deposition parameters for positive and negative lipid ion imaging using different matrices such as 2,5-dihydroxybenzoic acid (DHB), 9-aminoacridine (9-AA) or α-cyano-4-hydroxycinnamic acid (CHCA) have been optimized for the broadest detection and identification of renal lipids...
March 1, 2017: Talanta
https://www.readbyqxmd.com/read/28107753/role-of-central-opioid-on-the-antinociceptive-effect-of-sulfated-polysaccharide-from-the-red-seaweed-solieria-filiformis-in-induced-temporomandibular-joint-pain
#6
Ianna Wivianne Fernandes Araújo, Hellíada Vasconcelos Chaves, José Mário Pachêco, Danielle Rocha Val, Lorena Vasconcelos Vieira, Rodrigo Santos, Raul Sousa Freitas, Renata Line Rivanor, Valdécio Silvano Monteiro, Juliana Trindade Clemente-Napimoga, Mirna Marques Bezerra, Norma Maria Barros Benevides
This study aimed to investigate the effect of sulfated polysaccharide from red seaweed Solieria filiformis (Fraction F II) in the inflammatory hypernociception in the temporomandibular joint (TMJ) of rats. Male Wistar rats were pretreated (30min) with a subcutaneous injection (s.c.) of vehicle or FII (0.03, 0.3 or 3.0mg/kg) followed by intra-TMJ injection of 1.5% Formalin or 5-hydroxytryptamine (5-HT, 225μg/TMJ). In other set of experiments rats were pretreated (15min) with an intrathecal injection of the non-selective opioid receptors Naloxone, or μ-opioid receptor antagonist CTOP, or δ-opioid receptor Naltridole hydrochloride, or κ-opioid receptor antagonist Nor-Binaltorphimine (Nor-BNI) followed by injection of FII (s...
January 17, 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/28107678/nobiletin-and-its-colonic-metabolites-suppress-colitis-associated-colon-carcinogenesis-by-down-regulating-inos-inducing-antioxidative-enzymes-and-arresting-cell-cycle-progression
#7
Xian Wu, Mingyue Song, Zili Gao, Yue Sun, Minqi Wang, Fang Li, Jinkai Zheng, Hang Xiao
Nobiletin (NOB) is a major citrus polymethoxyflavone (PMF) with various beneficial biological activities. We reported previously that dietary NOB significantly inhibited colitis-associated colon carcinogenesis in azoxymethane (AOM)/dextran sulfate sodium (DSS)-treated mice, and the chemopreventive effects were associated with NOB metabolites found in the mouse colonic tissues. In this study, to better understand the role of colonic metabolites of NOB, we determined the anti-inflammation and anticancer effects of a mixture of NOB and its major metabolites (NOB-Met) at the concentrations equivalent to those found in colonic tissues of NOB-fed mice...
January 11, 2017: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/28107445/beta-glucans-supplementation-associates-with-reduction-in-p-cresyl-sulfate-levels-and-improved-endothelial-vascular-reactivity-in-healthy-individuals
#8
Carmela Cosola, Maria De Angelis, Maria Teresa Rocchetti, Eustacchio Montemurno, Valentina Maranzano, Giuseppe Dalfino, Carlo Manno, Annapaola Zito, Michele Gesualdo, Marco Matteo Ciccone, Marco Gobbetti, Loreto Gesualdo
BACKGROUND: Oat and barley beta-glucans are prebiotic fibers known for their cholesterol-lowering activity, but their action on the human gut microbiota metabolism is still under research. Although the induction of short-chain fatty acids (SCFA) following their ingestion has previously been reported, no study has investigated their effects on proteolytic uremic toxins p-cresyl sulfate (pCS) and indoxyl sulfate (IS) levels, while others have failed to demonstrate an effect on the endothelial function measured through flow-mediated dilation (FMD)...
2017: PloS One
https://www.readbyqxmd.com/read/28107390/melanoma-cell-adhesion-and-migration-is-modulated-by-the-uronyl-2-o-sulfotransferase
#9
Katerina Nikolovska, Dorothe Spillmann, Jörg Haier, Andrea Ladányi, Christian Stock, Daniela G Seidler
Although the vast majority of melanomas are characterized by a high metastatic potential, if detected early, melanoma can have a good prognostic outcome. However, once metastasised, the prognosis is bleak. We showed previously that uronyl-2-O sulfotransferase (Ust) and 2-O sulfation of chondroitin/dermatan sulfate (CS/DS) are involved in cell migration. To demonstrate an impact of 2-O sulfation in metastasis we knocked-down Ust in mouse melanoma cells. This significantly reduced the amount of Ust protein and enzyme activity...
2017: PloS One
https://www.readbyqxmd.com/read/28106668/primidone-inhibits-trpm3-and-attenuates-thermal-nociception-in-vivo
#10
Ute Krügel, Isabelle Straub, Holger Beckmann, Michael Schaefer
The melastatin-related transient receptor potential channel TRPM3 is a nonselective cation channel expressed in nociceptive neurons and activated by heat. Since TRPM3-deficient mice show inflammatory thermal hyperalgesia, pharmacological inhibition of TRPM3 may exert antinociceptive properties. Fluorometric Ca influx assays and a compound library containing approved or clinically tested drugs were used to identify TRPM3 inhibitors. Biophysical properties of channel inhibition were assessed using electrophysiological methods...
January 12, 2017: Pain
https://www.readbyqxmd.com/read/28105510/diversity-and-distribution-of-thermophilic-bacteria-in-hot-springs-of-pakistan
#11
Arshia Amin, Iftikhar Ahmed, Nimaichand Salam, Byung-Yong Kim, Dharmesh Singh, Xiao-Yang Zhi, Min Xiao, Wen-Jun Li
Chilas and Hunza areas, located in the Main Mantle Thrust and Main Karakoram Thrust of the Himalayas, host a range of geochemically diverse hot springs. This Himalayan geothermal region encompassed hot springs ranging in temperature from 60 to 95 °C, in pH from 6.2 to 9.4, and in mineralogy from bicarbonates (Tato Field), sulfates (Tatta Pani) to mixed type (Murtazaabad). Microbial community structures in these geothermal springs remained largely unexplored to date. In this study, we report a comprehensive, culture-independent survey of microbial communities in nine samples from these geothermal fields by employing a bar-coded pyrosequencing technique...
January 19, 2017: Microbial Ecology
https://www.readbyqxmd.com/read/28105173/interleukin-6-promotes-tumor-progression-in-colitis-associated-colorectal-cancer-through-hif-1%C3%AE-regulation
#12
Jun Han, Qiulei Xi, Qingyang Meng, Jingzheng Liu, Yongxian Zhang, Yusong Han, Qiulin Zhuang, Yi Jiang, Qiurong Ding, Guohao Wu
Interleukin-6 (IL-6) is a well-known etiological factor of colitis-associated colorectal cancer (CAC) and has a significant role in CAC progression. In addition, hypoxia-inducible factor 1α (HIF-1α) serves a primary role in the progression of CAC. However, the association between IL-6 and HIF-1α during the progression of CAC remains unclear. To investigate this association, the present study induced CAC in a mouse model using azoxymethane and dextran sulfate sodium. In addition, an anti-IL-6 receptor antibody was used to inhibit IL-6...
December 2016: Oncology Letters
https://www.readbyqxmd.com/read/28104982/mirna-133a-ucp2-pathway-regulates-inflammatory-bowel-disease-progress-by-influencing-inflammation-oxidative-stress-and-energy-metabolism
#13
Xi Jin, Dong Chen, Ruo-Heng Zheng, Hong Zhang, Yi-Peng Chen, Zun Xiang
AIM: To investigate the role of the miR-133a-UCP2 pathway in the pathogenesis of inflammatory bowel disease (IBD) and to explore the potential downstream mechanisms with respect to inflammation, oxidative stress and energy metabolism. METHODS: C57BL/6 mice were fed dextran sulfate sodium (DSS) liquid for 7 consecutive days, followed by the administration of saline to the DSS group, UCP2 siRNA to the UCP2 group and a miR-133a mimic to the miR-133a group on days 8 and 11...
January 7, 2017: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/28104889/a-peptide-hormone-required-for-casparian-strip-diffusion-barrier-formation-in-arabidopsis-roots
#14
Takuya Nakayama, Hidefumi Shinohara, Mina Tanaka, Koki Baba, Mari Ogawa-Ohnishi, Yoshikatsu Matsubayashi
Plants achieve mineral ion homeostasis by means of a hydrophobic barrier on endodermal cells called the Casparian strip, which restricts lateral diffusion of ions between the root vascular bundles and the soil. We identified a family of sulfated peptides required for contiguous Casparian strip formation in Arabidopsis roots. These peptide hormones, which we named Casparian strip integrity factor 1 (CIF1) and CIF2, are expressed in the root stele and specifically bind the endodermis-expressed leucine-rich repeat receptor kinase GASSHO1 (GSO1)/SCHENGEN3 and its homolog, GSO2...
January 20, 2017: Science
https://www.readbyqxmd.com/read/28104888/root-diffusion-barrier-control-by-a-vasculature-derived-peptide-binding-to-the-sgn3-receptor
#15
Verónica G Doblas, Elwira Smakowska-Luzan, Satoshi Fujita, Julien Alassimone, Marie Barberon, Mathias Madalinski, Youssef Belkhadir, Niko Geldner
The root endodermis forms its extracellular diffusion barrier by developing ringlike impregnations called Casparian strips. A factor responsible for their establishment is the SCHENGEN3/GASSHO1 (SGN3/GSO1) receptor-like kinase. Its loss of function causes discontinuous Casparian strips. SGN3 also mediates endodermal overlignification of other Casparian strip mutants. Yet, without ligand, SGN3 function remained elusive. Here we report that schengen2 (sgn2) is defective in an enzyme sulfating peptide ligands...
January 20, 2017: Science
https://www.readbyqxmd.com/read/28104792/new-insight-into-chondroitin-and-heparosan-like-capsular-polysaccharide-synthesis-by-profiling-of-the-nucleotide-sugar-precursors
#16
Odile Francesca Restaino, Irene di Lauro, Rosaria Di Nuzzo, Mario De Rosa, Chiara Schiraldi
Escherichia coli K4 and K5 capsular polysaccharides have been used as starting material for the biotechnological production of chondroitin sulfate and heparin, respectively. The capsular polysaccharide covers the outer cell wall but in late exponential or stationary growth phase it is started to be released in the surrounding medium. The released capsular polysaccharide concentration was used, so far, as the only marker to connect the strain production ability to the different cultivation conditions employed...
January 19, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28104786/identification-of-a-chondroitin-synthase-from-an-unexpected-source-the-green-sulfur-bacterium-chlorobium-phaeobacteroides
#17
Dixy E Green, Paul L DeAngelis
Glycosaminoglycans [GAGs] are known to be present in all animals as well as some pathogenic microbes. Chondroitin sulfate is the most abundant GAG in mammals where it has various structural and adhesion roles. The Gram-negative bacteria Pasteurella multocida Type F and Escherichia coli K4 produce extracellular capsules composed of unsulfated chondroitin or a fructosylated chondroitin, respectively. Such polysaccharides that are structurally related to host molecules do not generally provoke a strong antibody response thus are thought to be employed as molecular camouflage during infection...
January 19, 2017: Glycobiology
https://www.readbyqxmd.com/read/28104757/analysis-and-identification-of-the-grem2-heparin-heparan-sulfate-binding-motif
#18
Chandramohan Kattamuri, Kristof Nolan, Thomas B Thompson
Bone Morphogenetic Proteins (BMP) are regulated by extracellular antagonists of the DAN (differential screening selected gene aberrative in neuroblastoma) family. Similar to the BMP ligands, certain DAN family members have been shown to interact with heparin and heparan sulfate (HS). Structural studies of DAN family members Gremlin-1 and Gremlin-2 have revealed a dimeric growth-factor like fold where a series of lysine residues cluster along one face of the protein. In this study, we used mutagenesis, heparin binding measurements, and cell surface binding analysis to identify lysine residues that are important for heparin/HS binding in Gremlin-2...
January 19, 2017: Biochemical Journal
https://www.readbyqxmd.com/read/28104657/draft-genome-sequence-of-desulfovibrio-beroc1-a-mercury-methylating-strain
#19
Marisol Goñi Urriza, Claire Gassie, Oliver Bouchez, Christophe Klopp, Rémy Guyoneaud
Desulfovibrio BerOc1 is a sulfate-reducing bacterium isolated from the Berre lagoon (French Mediterranean coast). BerOc1 is able to methylate and demethylate mercury. The genome size is 4,081,579 bp assembled into five contigs. We identified the hgcA and hgcB genes involved in mercury methylation, but not those responsible for mercury demethylation.
January 19, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28104516/influence-of-uv-lamp-sulfur-iv-concentration-and-ph-on-bromate-degradation-in-uv-sulfite-systems-mechanisms-and-applications
#20
Qian Xiao, Ting Wang, Shuili Yu, Peng Yi, Lei Li
Bromate (BrO3(-)) is a possible human carcinogen regulated worldwide at a strict standard of 10 μg/L in drinking water. Removal of BrO3(-) by advanced reduction processes (ARPs) has attracted much attention due to its high reduction efficiency and easier combination with ultraviolet (UV) disinfection. In this study, we employed a UV/sulfite process to degrade BrO3(-) and studied the effects of UV lamp, sulfur(IV) concentration, and pH on effectiveness of the system in degrading BrO3(-). Low-pressure UV lamps (UV-L) instead of medium-pressure UV lamps (UV-M) were selected because of the high ultraviolet-C (UV-C) efficiency of UV-L...
January 11, 2017: Water Research
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