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Sulfotransferase

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https://www.readbyqxmd.com/read/29660372/heparan-sulfate-3-o-sulfotransferase-2-hs3st2-displays-an-unexpected-subcellular-localization-in-the-plasma-membrane
#1
Maxime Delos, François Foulquier, Charles Hellec, Dorothée Vicogne, Alexandre Fifre, Mathieu Carpentier, Dulce Papy-Garcia, Fabrice Allain, Agnès Denys
BACKGROUND: Heparan sulfate (HS) 3-O-sulfation can be catalysed by seven 3-O-sulfotransferases (HS3STs) in humans, still it is the rarest modification in HS and its biological function is yet misunderstood. HS3ST2 and HS3ST3B exhibit the same activity in vitro. They are however differently expressed in macrophages depending on cell environment, which suggests that they may be involved in distinct cellular processes. Here, we hypothesized that both isozymes might also display distinct subcellular localizations...
April 13, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29659839/airway-glycomic-and-allergic-inflammatory-consequences-resulting-from-keratan-sulfate-galactose-6-o-sulfotransferase-chst1-deficiency
#2
Tadahiro Kumagai, Takumi Kiwamoto, Mary E Brummet, Fan Wu, Kazuhiro Aoki, Zhou Zhu, Bruce S Bochner, Michael Tiemeyer
Siglec-F is a pro-apoptotic receptor on mouse eosinophils that recognizes 6'-sulfated sialyl Lewis X and 6'-sulfated sialyl N-acetyl-lactosamine as well as multivalent sialyl N-acetyl-lactosamine structures on glycan arrays. We hypothesized that attenuation of the carbohydrate sulfotransferase 1 (CHST1) gene encoding keratan sulfate galactose 6-O-sulfotransferase, an enzyme likely required for 6'-sulfation of some of these putative Siglec-F glycan ligands, would result in decreased Siglec-F lung ligand levels and enhanced allergic eosinophilic airway inflammation...
April 6, 2018: Glycobiology
https://www.readbyqxmd.com/read/29648993/serum-carbohydrate-sulfotransferase-7-in-lung-cancer-and-non-malignant-pulmonary-inflammations
#3
Željko Debeljak, Sandra Dundović, Sonja Badovinac, Sanja Mandić, Miroslav Samaržija, Branko Dmitrović, Marija Miloš, Lana Maričić, Vatroslav Šerić, Vikica Buljanović
BACKGROUND: Carbohydrate sulfotransferases (CHST) were shown to be involved in carcinogenesis. The aim of the study was to assess the diagnostic value of serum CHST7 concentration in differentiation between lung cancer and non-malignant pulmonary inflammations. METHODS: Clinical case-control study involving 125 participants was conducted: the control group containing cases of pneumonia and chronic obstructive pulmonary disease was compared to the lung cancer group composed of primary and metastatic cancers...
April 12, 2018: Clinical Chemistry and Laboratory Medicine: CCLM
https://www.readbyqxmd.com/read/29626075/sulfotransferase-4a1-sult4a1-increases-its-expression-in-mouse-neurons-as-they-mature
#4
Takuyu Hashiguchi, Sawako Shindo, Shih-Heng Chen, Jau-Shyong Hong, Masahiko Negishi
Cytosolic sulfotransferases (SULTs) catalyze sulfation and play essential roles in detoxification of xenobiotics as well as inactivation of endo-biotics. Sulfotransferase 4A1 (SULT4A1) which was originally isolated as a brain-specific sulfotransferase, is the most highly conserved isoform among SULTs in vertebrates. Here, expression of SULT4A1 was examined neuron enriched and neuron-glia mixed cells derived from mouse embryo brains at day 14 gestation and mixed glia from 2 days-old neonate brains. Western blots showed an increase of SULT4A1 expression as neurons maturated...
April 6, 2018: Drug Metabolism and Disposition: the Biological Fate of Chemicals
https://www.readbyqxmd.com/read/29621420/regulation-of-eosinophil-recruitment-and-allergic-airway-inflammation-by-heparan-sulfate-proteoglycan-hspg-modifying-enzymes
#5
Xiao Na Ge, Idil Bastan, Sung Gil Ha, Yana G Greenberg, Jeffrey D Esko, Savita P Rao, P Sriramarao
BACKGROUND: HSPGs are glycoproteins containing covalently attached heparan sulfate (HS) chains which bind to growth factors, chemokines, etc., and regulate various aspects of inflammation including cell recruitment. We previously showed that deletion of endothelial N-acetylglucosamine N-deacetylase-N-sulfotransferase-1 (Ndst1), an enzyme responsible for N-sulfation during HS biosynthesis, reduces allergic airway inflammation (AAI). Here, we investigated the importance of O-sulfation mediated by uronyl 2-O-sulfotransferase (Hs2st) in development of AAI relative to N-sulfation...
April 5, 2018: Experimental Lung Research
https://www.readbyqxmd.com/read/29619629/effect-of-kynurenic-acid-on-pupae-viability-of-drosophila-melanogaster-cinnabar-and-cardinal-eye-color-mutants-with-altered-tryptophan-kynurenine-metabolism
#6
Valeriya Navrotskaya, Artur Wnorowski, Waldemar Turski, Gregory Oxenkrug
Kynurenic acid (KYNA) is one of the metabolites of evolutionary conserved tryptophan (Trp)/kynurenine (Kyn) metabolic pathway. Elevation of KYNA contributes to development of psychosis in schizophrenia but attenuates neurodegeneration in Drosophila model of Huntington's disease. We have reported that KYNA increased lethality of pupae of wild-type flies, but not of vermilion (v) mutants with impaired formation of Kyn from Trp, suggesting that KYNA toxicity depends on its interaction with downstream Kyn metabolites [i...
April 4, 2018: Neurotoxicity Research
https://www.readbyqxmd.com/read/29596660/metabolic-activation-of-the-cooked-meat-carcinogen-2-amino-1-methyl-6-phenylimidazo-4-5-b-pyridine-in-human-prostate
#7
Medjda Bellamri, Shun Xiao, Paari Murugan, Christopher J Weight, Robert J Turesky
2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP), an heterocyclic aromatic amine (HAA) formed in cooked meat, is a rodent and possible human prostate carcinogen. Recently, we identified DNA adducts of PhIP in the genome of prostate cancer patients, but adducts of 2-amino-3,8-dimethylmidazo[4,5-f]quinoxaline (MeIQx) and 2-amino-9H-pyrido[2,3-b]indole (AαC), other prominent HAAs formed in cooked meats, were not detected. We have investigated the bioactivation of HAAs by Phase I and II enzymes in the human prostate (LNCaP) cell line using cytotoxicity and DNA adducts as endpoints...
March 27, 2018: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/29589903/changes-in-protein-dynamics-in-escherichia-coli-sufs-reveal-a-possible-conserved-regulatory-mechanism-in-type-ii-cysteine-desulfurase-systems
#8
Dokyong Kim, Harsimran Singh, Yuyuan Dai, Guangchao Dong, Laura S Busenlehner, Franklin Wayne Outten, Patrick A Frantom
In the Suf Fe-S cluster assembly pathway, the activity of the cysteine desulfurase, SufS, is regulated by interactions with the accessory sulfotransferase protein, SufE. SufE has been shown to stimulate SufS activity, likely through inducing conformational changes in the SufS active site that promote the desulfurase step and by acting as an efficient persulfide acceptor in the transpersulfuration step. Previous results point toward an additional level of regulation through a 'half-sites' mechanism that affects the stoichiometry and affinity for SufE as the dimeric SufS shifts between desulfurase and transpersulfuration activities...
March 28, 2018: Biochemistry
https://www.readbyqxmd.com/read/29588428/expression-of-sult2a1-sult2b1-and-hsd3b1-in-the-porcine-testis-and-epididymis
#9
Bettina Zimmer, Linda Tenbusch, Michele C Klymiuk, Yaser Dezhkam, Gerhard Schuler
In the porcine testis in addition to estrogen sulfates the formation of numerous sulfonated neutral hydroxysteroids has been observed. However, their functions and the underlying synthetic pathways are still widely unclear. To obtain further information on their formation in postpubertal boars the expression of sulfotransferases considered relevant for neutral hydroxysteroids (SULT2A1, SULT2B1) was investigated in the testis and defined segments of the epididymis applying real-time RT-qPCR, Western blot and immunohistochemistry (IHC)...
March 27, 2018: Journal of Molecular Endocrinology
https://www.readbyqxmd.com/read/29581787/sult1e1-inhibits-cell-proliferation-and-invasion-by-activating-ppar%C3%AE-in-breast-cancer
#10
Yali Xu, Xiaoyan Lin, Jiawen Xu, Haiyan Jing, Yejun Qin, Yintao Li
Sulfotransferase family 1E member 1 (SULT1E1) is known to catalyze sulfoconjugation and play a crucial role in the deactivation of estrogen homeostasis, which is involved in tumorigenesis and the progression of breast and endometrial cancers. Our previous study has shown that the protein levels of SULT1E1 were decreased in breast cancer; however, the underlying mechanism is still poorly understood. In this study, we explored the functional and molecular mechanisms by which SULT1E1 influenced breast cancer. Here, we identified that overexpression of SULT1E1 inhibited breast cancer cell growth through inducing apoptosis and arresting cell cycle progression...
2018: Journal of Cancer
https://www.readbyqxmd.com/read/29559501/synaptogenesis-is-modulated-by-heparan-sulfate-in-caenorhabditis-elegans
#11
María Lázaro-Peña, Carlos Díaz-Balzac, Hannes Bülow, Scott Emmons
The nervous system regulates complex behaviors through a network of neurons interconnected by synapses. How specific synaptic connections are genetically determined is still unclear. Male mating is the most complex behavior in C. elegans It is composed of sequential steps that are governed by more than 3,000 chemical connections. Here we show that heparan sulfates (HS) play a role in the formation and function of the male neural network. HS, sulfated in position 3 by the HS modification enzyme HST-3.1 / HS 3- O -sulfotransferase and attached to the HSPG glypicans LON-2/glypican and GPN-1/glypican, functions cell-autonomously and non-autonomously for response to hermaphrodite contact during mating...
March 20, 2018: Genetics
https://www.readbyqxmd.com/read/29547633/the-heparan-sulfate-3-o-sulfotransferases-hs3st-2-3b-and-4-enhance-proliferation-and-survival-in-breast-cancer-mda-mb-231-cells
#12
Charles Hellec, Maxime Delos, Mathieu Carpentier, Agnès Denys, Fabrice Allain
Heparan sulfate 3-O-sulfotransferases (HS3STs) catalyze the final maturation step of heparan sulfates. Although seven HS3ST isozymes have been described in human, 3-O-sulfation is a relatively rare modification, and only a few biological processes have been described to be influenced by 3-O-sulfated motifs. A conflicting literature has recently reported that HS3ST2, 3A, 3B and 4 may exhibit either tumor-promoting or anti-oncogenic properties, depending on the model used and cancer cell phenotype. Hence, we decided to compare the consequences of the overexpression of each of these HS3STs in the same cellular model...
2018: PloS One
https://www.readbyqxmd.com/read/29521245/designing-ligands-for-leishmania-plasmodium-and-aspergillus-n-myristoyl-transferase-with-specificity-and-anti-target-safe-virtual-libraries
#13
Alfonso T Garcia-Sosa
Leishmaniasis, malaria, and fungal diseases are burdens on individuals and populations and can present severe complications. Easily accessible chemical treatments for these diseases are increasingly sought-after. Targeting the parasite N-myristoyl transferase while avoiding the human enzyme and other anti-targets may allow the prospect of compounds with pan-activity against these diseases, which would simplify treatments and costs. Developing chemical libraries, both virtual and physical, that have been filtered and flagged early on in the drug discovery process (before virtual screening) could reduce attrition rates of compounds being developed and failing late in development stages due to problems of side-effects or toxicity...
March 8, 2018: Current Computer-aided Drug Design
https://www.readbyqxmd.com/read/29516954/heparan-sulfate-d-glucosamine-3-o-sulfotransferase-3b1-is-a-novel-regulator-of-transforming-growth-factor-beta-mediated-epithelial-to-mesenchymal-transition-and-regulated-by-mir-218-in-nonsmall-cell-lung-cancer
#14
Zengli Zhang, Hanyi Jiang, Yongsheng Wang, Minhua Shi
Background: Due to high metastasis and recurrence rate. Recent studies indicated that epithelial-to-mesenchymal transition (EMT) was involved in the progression and metastasis in cancer. Some reports also indicate that HS3ST3B1 played a role in angiogenesis and the proliferation of cancer cells. In this study, we aim to investigate its role in non-small cell lung cancer (NSCLC) . Materials and Methods: All cell lines were purchased from ATCC and cultured in our central lab...
January 2018: Journal of Cancer Research and Therapeutics
https://www.readbyqxmd.com/read/29514872/homozygous-chst11-mutation-in-chondrodysplasia-brachydactyly-overriding-digits-clino-symphalangism-and-synpolydactyly
#15
Rana Muhammad Kamran Shabbir, Gökhan Nalbant, Nafees Ahmad, Sajid Malik, Aslıhan Tolun
BACKGROUND: Carbohydrate sulfotransferase 11 (CHST11) is a membrane protein of Golgi that catalyses the transfer of sulfate to position 4 of the N-acetylgalactosamine residues of chondroitin. Chondroitin sulfate is the predominant proteoglycan in cartilage, and its sulfation is important in the developing growth plate of cartilage. A homozygous deletion encompassing part of the gene and the embedded miRNA MIR3922 had been detected in a woman with hand/foot malformation and malignant lymphoproliferative disease...
March 7, 2018: Journal of Medical Genetics
https://www.readbyqxmd.com/read/29503429/-metabolism-of-sesamin-and-drug-sesamin-interaction
#16
Toshiyuki Sakaki, Kaori Yasuda, Miyu Nishikawa, Shinichi Ikushiro
 Sesamin, derived from sesame seeds, is known to have various biological effects. Since some of these effects appear to be derived from its metabolites, the elucidation of sesamin metabolism is essential to understanding the molecular mechanism of its effects. In addition, it is important to clarify drug-sesamin interactions in order to address safety concerns, as some food factors are known to affect drug metabolism. Our previous studies revealed that sesamin was sequentially metabolized by cytochrome P450 (CYP) and UDP-glucuronosyltransferase or sulfotransferase...
2018: Yakugaku Zasshi: Journal of the Pharmaceutical Society of Japan
https://www.readbyqxmd.com/read/29484646/a-microbial-enzymatic-strategy-for-producing-chondroitin-sulfate-glycosaminoglycans
#17
Zhengxiong Zhou, Qing Li, Hao Huang, Hao Wang, Yang Wang, Guocheng Du, Jian Chen, Zhen Kang
Chondroitin sulfate has been widely used in both medical and clinical applications. Commercial chondroitin sulfate has been mainly acquired from animal tissue extraction. Here we report a new two-step biological strategy for producing chondroitin sulfate A and chondroitin sulfate C. First, the chondroitin biosynthesis pathway in a recombinant Bacillus subtilis strain using sucrose as carbon source was systematically optimized and the titer of chondroitin was significantly enhanced to 7.15 g/L. Then, specific sulfation transformation systems were successfully constructed and optimized by combining the purified aryl sulfotransferase IV (ASST IV), chondroitin 4-sulfotransferase (C4ST) and chondroitin 6-sulfotransferase (C6ST)...
February 27, 2018: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/29467139/formation-and-hydrolysis-of-sulfonated-estrogens-in-the-porcine-testis-and-epididymis
#18
Gerhard Schuler, Yaser Dezhkam, Linda Tenbusch, Michele C Klymiuk, Bettina Zimmer, Bernd Hoffmann
Boars exhibit high concentrations of sulfonated estrogens (SE) mainly originating from the testicular-epididymal compartment. Intriguingly, in porcine Leydig cells sulfonation of estrogens is colocalized with aromatase and steroid sulfatase (STS), indicating that de novo synthesis of unconjugated estrogens (UE), their sulfonation and hydrolysis of SE occur within the same cell type. So far in boars no plausible concept concerning the role of SE has been put forward. To obtain new information on SE formation and hydrolysis the porcine testicular-epididymal compartment was screened for the expression of the estrogen specific sulfotransferase SULT1E1 and STS applying real-time RT-qPCR, Western Blot and immunohistochemistry...
February 21, 2018: Journal of Molecular Endocrinology
https://www.readbyqxmd.com/read/29445054/comparison-of-drug-metabolism-and-its-related-hepatotoxic-effects-in-heparg-cryopreserved-human-hepatocytes-and-hepg2-cell-cultures
#19
Yuichi Yokoyama, Yoshifumi Sasaki, Natsuko Terasaki, Taku Kawataki, Koji Takekawa, Yumiko Iwase, Toshinobu Shimizu, Seigo Sanoh, Shigeru Ohta
Differentiated HepaRG cells maintain liver-specific functions such as drug-metabolizing enzymes. In this study, the feasibility of HepaRG cells as a human hepatocyte model for in vitro toxicity assessment was examined using selected hepatotoxic compounds. First, basal drug-metabolizing enzyme activities (CYP1A2, CYP2B6, CYP2C9, CYP2C19, CYP2D6, CYP3A4, uridine 5'-diphospho-glucuronosyltransferase [UGT], and sulfotransferases [SULT]) were measured in HepaRG, human hepatocytes, and HepG2 cells. Enzyme activities in differentiated HepaRG cells were comparable to those in human hepatocytes and much higher than those in HepG2 cells, except for SULT activity...
February 14, 2018: Biological & Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/29440314/the-steroid-sulfate-axis-and-its-relationship-to-maternal-behavior-and-mental-health
#20
William Davies
Steroid hormones can exist in functionally-dissociable sulfated and non-sulfated (free) forms and can exert profound effects on numerous aspects of mammalian physiology; the ratio of free to sulfated steroids is governed by the antagonistic actions of steroid sulfatase (STS) and sulfotransferase (SULT) enzymes. Here, I examine evidence from human and animal model studies which suggests that STS and its major substrate (dehydroepiandrosterone sulfate, DHEAS) and product (DHEA) can influence brain function, behavior and mental health, before summarising how the activity of this axis varies throughout mammalian pregnancy and the postpartum period...
February 13, 2018: Journal of Molecular Endocrinology
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