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https://www.readbyqxmd.com/read/29038294/the-nsaid-allosteric-site-of-human-cytosolic-sulfotransferases
#1
Ting Wang, Ian Cook, Thomas S Leyh
Non-steroidal anti-inflammatory drugs (NSAIDs) are among the most commonly prescribed drugs worldwide - more than 111 million prescriptions were written in the United Sates in 2014. NSAIDs allosterically inhibit cytosolic sulfotransferases (SULTs) with high specificity and therapeutically relevant affinities. The current study focuses on the interactions of SULT1A1 and mefenamic acid (MEF) - a potent, highly specific NSAID inhibitor of 1A1. Here, the first structure of an NSAID allosteric site - the MEF-binding site of SULT1A1 - is determined using spin-label-triangulation NMR...
October 16, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29031804/transcriptional-response-after-exposure-to-domoic-acid-producing-pseudo-nitzschia-in-the-digestive-gland-of-the-mussel-mytilus-galloprovincialis
#2
Antonio J Pazos, Pablo Ventoso, Roi Martínez-Escauriaza, M Luz Pérez-Parallé, Juan Blanco, Juan C Triviño, José L Sánchez
Bivalve molluscs are filter feeding species that can accumulate biotoxins in their body tissues during harmful algal blooms. Amnesic Shellfish Poisoning (ASP) is caused by species of the diatom genus Pseudo-nitzschia, which produces the toxin domoic acid. The Mytilus galloprovincialis digestive gland transcriptome was de novo assembled based on the sequencing of 12 cDNA libraries, six obtained from control mussels and six from mussels naturally exposed to domoic acid-producing diatom Pseudo-nitzschia australis...
October 11, 2017: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/29025375/dopamine-induced-sult1a3-4-promotes-emt-and-cancer-stemness-in-hepatocellular-carcinoma
#3
Juan Zou, Hong Li, Qianling Huang, Xiaomin Liu, Xiaoxiao Qi, Ying Wang, Linlin Lu, Zhongqiu Liu
Hepatocellular carcinoma has the second highest incidence rate among malignant cancers in China. Hepatocellular carcinoma development is complex because of the metabolism disequilibrium involving SULT1A3/4, a predominant sulfotransferase that metabolizes sulfonic xenobiotics and endogenous catecholamines. However, the correlation between SULT1A3/4 and hepatocellular carcinoma progression is unclear. By utilizing immunofluorescence and immunohistochemical analysis, we found that in nine hepatocellular carcinoma clinical specimens, SULT1A3/4 was abundantly expressed in tumor tissues compared to that in the adjacent tissues...
October 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/29022574/osthole-prevents-acetaminophen-induced-liver-injury-in-mice
#4
Yun Cai, Wu Sun, Xin-Xin Zhang, Yan-Die Lin, Hao Chen, Hao Li
Acetaminophen (APAP) overdose leads to severe hepatotoxicity. Osthole, a natural coumarin found in traditional Chinese medicinal herbs, has therapeutic potential in the treatment of various diseases. In this study, we investigated the effects of osthole against APAP-induced hepatotoxicity in mice. Mice were administered osthole (100 mg·kg(-1)·d(-1), ip) for 3 d, then on the fourth day APAP (300 mg/kg, ip) was co-administered with osthole. The mice were euthanized post-APAP, their serum and livers were collected for analysis...
October 12, 2017: Acta Pharmacologica Sinica
https://www.readbyqxmd.com/read/28983631/integrated-analysis-reveals-candidate-genes-and-transcription-factors-in-lung-adenocarcinoma
#5
Baiwang Chen, Shuhong Gao, Changwei Ji, Ge Song
Lung adenocarcinoma is the most common type of non‑small cell lung cancer in Asia. Therefore, it is important to improve understanding of the underlying transcriptional regulatory mechanisms involved. The present study aimed to identify potential candidate genes and transcription factors (TFs) associated with the disease. Four gene expression profiles were downloaded from the Gene Expression Omnibus database, which included 141 lung adenocarcinoma patients and 191 healthy controls. The differentially expressed genes (DEGs) were screened out and functional annotation was performed...
September 28, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28970022/stereoselective-degradation-and-thyroid-endocrine-disruption-of-lambda-cyhalothrin-in-lizards-eremias-argus-following-oral-exposure
#6
Jing Chang, Weiyu Hao, Yuanyuan Xu, Peng Xu, Wei Li, Jianzhong Li, Huili Wang
The disturbance of the thyroid system and elimination of chiral pyrethroid pesticides with respect to enantioselectivity in reptiles have so far received limited attention by research. In this study, bioaccumulation, thyroid gland lesions, thyroid hormone levels, and hypothalamus-pituitary-thyroid axis-related gene expression in male Eremias argus were investigated after three weeks oral administration of lambda-cyhalothrin (LCT) enantiomers. In the lizard liver, the concentration of LCT was negatively correlated with the metabolite-3-phenoxybenzoic acid (PBA) level during 21 days of exposure...
September 29, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28939687/rna-seq-profiling-of-intestinal-expression-of-xenobiotic-processing-genes-in-germ-free-mice
#7
Zidong Donna Fu, Felcy P Selwyn, Julia Yue Cui, Curtis D Klaassen
Intestinal bacteria can affect xenobiotic metabolism through both direct bacterial enzyme-catalyzed modification of the xenobiotics and indirect alterations of the expression of host genes. In order to determine the effect of the lack of intestinal bacteria on the expression of xenobiotic-processing genes (XPGs) of the host intestine, the mRNA profiles of 303 XPGs were characterized by high-throughput RNA-sequencing in four sections of the intestine and compared to that in the liver from adult male conventional (CV) and germ-free (GF) mice...
September 22, 2017: Drug Metabolism and Disposition: the Biological Fate of Chemicals
https://www.readbyqxmd.com/read/28938414/sex-and-tissue-specific-role-of-estrogen-sulfotransferase-in-energy-homeostasis-and-insulin-sensitivity
#8
Wojciech G Garbacz, Mengxi Jiang, Meishu Xu, Jun Yamauchi, H Henry Dong, Wen Xie
Estrogen Sulfotransferase (EST) catalyzes the sulfoconjugation and deactivation of estrogens. Previously we showed that loss of Est in male ob/ob mice, but not in female ob/ob mice, exacerbated the diabetic phenotype, but the underlying mechanism was unclear. Here we show that transgenic reconstitution of Est in the adipose tissue, but not in the liver, attenuated diabetic phenotype in Est deficient ob/ob mice (obe). Mechanistically, adipose reconstitution of Est in obe mice (oae) resulted in reduced local and systemic inflammation, improved insulin sensitivity, and increased energy expenditure...
September 5, 2017: Endocrinology
https://www.readbyqxmd.com/read/28933499/multi-biomarker-approach-in-the-scallop-chlamys-farreri-to-assess-pahs-pollution-in-qingdao-coastal-areas-of-china
#9
Luqing Pan, Mengyu Zhang, Qian Jin, Rongwang Ji
A multi-biomarker approach was conducted in the scallop Chlamys farreri from three sites, denoted here as S1, S2, and S3, in Qingdao coastal areas of China in March, June, September and December 2014 to assess pollution from polycyclic aromatic hydrocarbons (PAHs) and to select appropriate biomarkers. A suite of biological responses of the gills and digestive glands of the scallops was assayed, including: (i) phase I detoxification enzymes of 7-ethoxyresorufin-O-deethylase (EROD), epoxide hydrolase (EH), and dihydrodiol dehydrogenase (DD) and phase II detoxification enzymes of glutathione-S-transferase (GST) and sulfotransferase (SULT); (ii) antioxidant enzymes: catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx); (iii) oxidative damage parameters: lipid peroxidation (LPO) expressed by malondialdehyde (MDA) contents, protein carbonylation (PC) and DNA damage (F value); and (iv) the metabolism-related genes of EH, DD, GST, SULT and SOD...
September 21, 2017: Environmental Science. Processes & Impacts
https://www.readbyqxmd.com/read/28912067/farnesoid-x-receptor-regulates-sult1e1-expression-through-inhibition-of-pgc1%C3%AE-binding-to-hnf4%C3%AE
#10
Shuai Wang, Xue Yuan, Danyi Lu, Lianxia Guo, Baojian Wu
Sulfotransferase 1E1 (SULT1E1, also known as estrogen sulfotransferase) plays an important role in metabolism and detoxification of many endogenous and exogenous compounds (e.g., estrogens and flavonoids). Here we aimed to assess the effects of farnesoid X receptor (FXR) activation on SULT1E1 expression, and to determine the mechanism thereof. Treatment with specific FXR agonists (i.e., GW4064 and CDCA) significantly decreased both mRNA and protein levels of SULT1E1 in HepG2 cells. This was accompanied by a decrease in the enzymatic activity...
September 11, 2017: Biochemical Pharmacology
https://www.readbyqxmd.com/read/28884488/uniqueness-of-entamoeba-sulfur-metabolism-sulfolipid-metabolism-that-plays-pleiotropic-roles-in-the-parasitic-life-cycle
#11
Fumika Mi-Ichi, Tomofumi Miyamoto, Hiroki Yoshida
Sulfur metabolism is ubiquitous and terminally synthesizes various biomolecules that are crucial for organisms, such as sulfur-containing amino acids and co-factors, sulfolipids, and sulfated saccharides. Entamoeba histolytica, a protozoan parasite responsible for amoebiasis, possesses the unique sulfur metabolism features of atypical localization and its terminal product being limited to sulfolipids. Here, we present an overall scheme of E. histolytica sulfur metabolism by relating all sulfotransferases and sulfatases to their substrates and products...
September 7, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/28875717/sult1a1-copy-number-variation-ethnic-distribution-analysis-in-an-indian-population
#12
Suhani Almal, Harish Padh
Cytosolic sulfotransferases (SULTs) are phase II detoxification enzymes involved in metabolism of numerous xenobiotics, drugs and endogenous compounds. Interindividual variation in sulfonation capacity is important for determining an individual's response to xenobiotics. SNPs in SULTs, mainly SULT1A1 have been associated with cancer risk and also with response to therapeutic agents. Copy number variation (CNVs) in SULT1A1 is found to be correlated with altered enzyme activity. This short report primarily focuses on CNV in SULT1A1 and its distribution among different ethnic populations around the globe...
September 24, 2017: Annals of Human Biology
https://www.readbyqxmd.com/read/28873964/the-pearl-oyster-pinctada-fucata-martensii-genome-and-multi-omic-analyses-provide-insights-into-biomineralization
#13
Xiaodong Du, Guangyi Fan, Yu Jiao, He Zhang, Ximing Guo, Ronglian Huang, Zhe Zheng, Chao Bian, Yuewen Deng, Qingheng Wang, Zhongduo Wang, Xinming Liang, Haiying Liang, Chengcheng Shi, Xiaoxia Zhao, Fengming Sun, Ruijuan Hao, Jie Bai, Jialiang Liu, Wenbin Chen, Jinlian Liang, Weiqing Liu, Zhe Xu, Qiong Shi, Xun Xu, Guofan Zhang, Xin Liu
Nacre, the iridescent material found in pearls and shells of molluscs, is formed through an extraordinary process of matrix-assisted biomineralization. Despite recent advances, many aspects of the biomineralization process and its evolutionary origin remain unknown. The pearl oyster Pinctada fucata martensii is a well-known master of biomineralization, but the molecular mechanisms that underlie its production of shells and pearls are not fully understood. We sequenced the highly polymorphic genome of the pearl oyster and conducted multi-omic and biochemical studies to probe nacre formation...
August 1, 2017: GigaScience
https://www.readbyqxmd.com/read/28867356/relationship-of-sult1a1-copy-number-variation-with-estrogen-metabolism-and-human-health
#14
Jixia Liu, Ran Zhao, Zhan Ye, Alexander J Frey, Emily R Schriver, Nathaniel W Snyder, Scott J Hebbring
Human cytosolic sulfotransferase 1A1 (SULT1A1) is considered to be one of the most important SULT isoforms for metabolism, detoxification, and carcinogenesis. This theory is driven by observations that SULT1A1 is widely expressed in multiple tissues and acts on a wide range of phenolic substrates. SULT1A1 is subject to functional common copy number variation (CNV) including deletions or duplications. However, it is less clear how SULT1A1 CNV impacts health and disease. To better understand the biological role of SULT1A1 in human health, we genotyped CNV in 14,275 Marshfield Clinic patients linked to an extensive electronic health record...
August 31, 2017: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/28866654/hydroxylation-and-sulfation-of-sex-steroid-hormones-in-inflammatory-liver
#15
Sang R Lee, Seung-Yeon Lee, Sang-Yun Kim, Si-Yun Ryu, Bae-Kuen Park, Eui-Ju Hong
Sex steroids, also known as gonadal steroids, are oxidized with hydroxylation by cytochrome P450, glucuronidation by UDP-glucuronosyltransferase, sulfation by sulfotransferase, andO-methylation by catechol O-methyltransferase. Thus, it is important to determine the process by which inflammation influences metabolism of gonadal hormones. Therefore, we investigated the mechanism of metabolic enzymes against high physiologic inflammatory responsein vivo to study their biochemical properties in liver diseases. In this study, C57BL/6N mice were induced with hepatic inflammation by diethylnitrosamine (DEN) exposure...
September 26, 2017: Journal of Biomedical Research
https://www.readbyqxmd.com/read/28863307/transcriptomic-characterization-of-bovine-primary-cultured-hepatocytes-a-cross-comparison-with-a-bovine-liver-and-the-madin-darby-bovine-kidney-cells
#16
Ramy Elgendy, Mery Giantin, Mauro Dacasto
Bovine primary cultured hepatocytes (CHs) are widely used in vitro models for liver toxicity testing. However, little is known about their whole-transcriptome profile and its resemblance to the normal liver tissue. In the present study, we profiled - by microarray - the whole-transcriptome of bovine CHs (n=4) and compared it with the transcriptomic landscape of control liver samples (n=8), as well the Madin-Darby bovine kidney (MDBK) cells (n=4). Compared with liver tissue, the bovine CHs relatively expressed (fold change >2, P<0...
August 8, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28821720/structural-basis-for-the-broad-substrate-specificity-of-the-human-tyrosylprotein-sulfotransferase-1
#17
Shinnosuke Tanaka, Toshiaki Nishiyori, Hidetaka Kojo, Reo Otsubo, Moe Tsuruta, Katsuhisa Kurogi, Ming-Cheh Liu, Masahito Suiko, Yoichi Sakakibara, Yoshimitsu Kakuta
Tyrosylprotein sulfotransferases (TPSTs) are enzymes that catalyze post-translational tyrosine sulfation of proteins. In humans, there are only two TPST isoforms, designated TPST1 and TPST2. In a previous study, we reported the crystal structure of TPST2, which revealed the catalytic mechanism of the tyrosine sulfation reaction. However, detailed molecular mechanisms underlying how TPSTs catalyse a variety of substrate proteins with different efficiencies and how TPSTs catalyze the sulfation of multiple tyrosine residues in a substrate protein remain unresolved...
August 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28807679/identification-and-characterization-of-5%C3%AE-cyprinol-sulfating-cytosolic-sulfotransferases-sults-in-the-zebrafish-danio-rerio
#18
Katsuhisa Kurogi, Maki Yoshihama, Austin Horton, Isaac T Schiefer, Matthew D Krasowski, Lee R Hagey, Frederick E Williams, Yoichi Sakakibara, Naoya Kenmochi, Masahito Suiko, Ming-Cheh Liu
5α-Cyprinol 27-sulfate is the major biliary bile salt present in cypriniform fish including the zebrafish (Danio rerio). The current study was designed to identify the zebrafish cytosolic sulfotransferase (Sult) enzyme(s) capable of sulfating 5α-cyprinol and to characterize the zebrafish 5α-cyprinol-sulfating Sults in comparison with human SULT2A1. Enzymatic assays using zebrafish homogenates showed 5α-cyprinol-sulfating activity. A systematic analysis, using a panel of recombinant zebrafish Sults, revealed two Sult2 subfamily members, Sult2st2 and Sult2st3, as major 5α-cyprinol-sulfating Sults...
August 11, 2017: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/28802998/on-the-sulfation-of-o-desmethyltramadol-by-human-cytosolic-sulfotransferases
#19
Mohammed I Rasool, Ahsan F Bairam, Katsuhisa Kurogi, Ming-Cheh Liu
BACKGROUND: Previous studies have demonstrated that sulfate conjugation is involved in the metabolism of the active metabolite of tramadol, O-desmethyltramadol (O-DMT). The current study aimed to systematically identify the human cytosolic sulfotransferases (SULTs) that are capable of mediating the sulfation of O-DMT. METHODS: The sulfation of O-DMT under metabolic conditions was demonstrated using HepG2 hepatoma cells and Caco-2 human colon carcinoma cells. O-DMT-sulfating activity of thirteen known human SULTs and four human organ specimens was examined using an established sulfotransferase assay...
February 16, 2017: Pharmacological Reports: PR
https://www.readbyqxmd.com/read/28797077/gender-difference-in-nash-susceptibility-roles-of-hepatocyte-ikk%C3%AE-and-sult1e1
#20
Noriko Matsushita, Mohamed T Hassanein, Marcos Martinez-Clemente, Raul Lazaro, Samuel W French, Wen Xie, Keane Lai, Michael Karin, Hidekazu Tsukamoto
Myeloid cell and hepatocyte IKKβ may mediate the genesis of obesity and insulin resistance in mice fed high fat diet. However, their gender-specific roles in the pathogenesis of non-alcoholic steatohepatitis (NASH) are not known. Here we demonstrate myeloid IKKβ deficiency prevents Western diet-induced obesity and visceral adiposity in females but not in males, and attenuates hyperglycemia, global IR, and NASH in both genders. In contrast, all metabolic sequela including NASH are aggravated by hepatocyte IKKβ deficiency (IkbkbΔhep) in male but not female mice...
2017: PloS One
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