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https://www.readbyqxmd.com/read/28941417/protective-role-of-liriodendrin-in-mice-with-dextran-sulphate-sodium-induced-ulcerative-colitis
#1
Zhen Zhang, Lei Yang, Botao Wang, Lanqiu Zhang, Qi Zhang, Dihua Li, Shukun Zhang, Hongwei Gao, Ximo Wang
Sargentodoxa cuneata, containing syringaresinol and its glycoside liriodendrin as the main bioactive compounds, is a well-known traditional Chinese medicine for treating intestinal inflammation. In our preliminary study, liriodendrin inhibited NF-kB activation in sepsis-induced acute lung injury. The present study was designed to investigate its effect on dextran sulfate sodium (DSS)-induced colitis in a mouse model and to explore the possible related mechanisms. Experimental colitis was established by giving mice drinking water containing 3% (w/v) DSS for 7days...
September 20, 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/28938606/oroxylin-a-inhibits-colitis-by-inactivating-nlrp3-inflammasome
#2
Wei Zhou, Xiuting Liu, Xin Zhang, Jingjing Tang, Zhiyu Li, Qing Wang, Rong Hu
NLRP3 inflammasome is a novel therapeutic target for inflammatory bowel disease (IBD). The aim of this study was to investigate the anti-inflammatory effect of a bioactive flavonoid-oroxylin A on the treatment of dextran sulfate sodium (DSS)-induced murine colitis via targeting NLRP3 inflammasome. In this study, we found that oroxylin A attenuated experimental colitis in mice, including loss of body weights, shortening of the colon lengths and infiltration of inflammatory cells. The production of IL-1β, IL-6 and TNF-α in colon was also markedly reduced by oroxylin A...
August 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/28938381/accelerated-epi-on-versus-standard-epi-off-corneal-collagen-cross-linking-for-progressive-keratoconus-in-pediatric-patients
#3
Maria A Henriquez, Ana M Rodríguez, Luis Izquierdo
PURPOSE: To evaluate and compare the effectiveness and safety of accelerated transepithelial (A-epi-on) corneal collagen cross-linking (CXL) with standard CXL (epi-off) for children with progressive keratoconus. METHODS: Prospective, cohort study including 61 eyes of 51 patients younger than 18 years with progressive keratoconus at Oftalmosalud Instituto de Ojos, Lima, Peru. A-epi-on CXL was performed for 36 eyes using 30 minutes of impregnation (0.25% riboflavin, 1...
September 21, 2017: Cornea
https://www.readbyqxmd.com/read/28938014/intestinal-helminth-infection-drives-carcinogenesis-in-colitis-associated-colon-cancer
#4
Eva Pastille, Annika Frede, Henry J McSorley, Jessica Gräb, Alexandra Adamczyk, Sebastian Kollenda, Wiebke Hansen, Matthias Epple, Jan Buer, Rick M Maizels, Robert Klopfleisch, Astrid M Westendorf
Inflammatory bowel diseases (IBD) are chronic inflammatory disorders of the gastrointestinal tract, strongly associated with an increased risk of colorectal cancer development. Parasitic infections caused by helminths have been shown to modulate the host's immune response by releasing immunomodulatory molecules and inducing regulatory T cells (Tregs). This immunosuppressive state provoked in the host has been considered as a novel and promising approach to treat IBD patients and alleviate acute intestinal inflammation...
September 22, 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28937245/dextran-sodium-sulphate-dss-induced-colitis-alters-the-expression-of-neurotrophins-in-smooth-muscle-cells-of-rat-colon
#5
M Al-Qudah, D A Shammala, A Al-Dwairi, O Al-Shboul, A G Mustafa
Neurotrophins are present in the gastrointestinal tract where they participate in the survival and growth of enteric neurons, augmentation of enteric circuits, elevation of colonic myoelectrical activity and also in different aspects of colitis. Previous studies largely focused on the role of neural and mucosal neurotrophins in gut inflammation. The expression of neurotrophins in colonic smooth muscle cells (SMCs) and the interactions of this potential source with colitis has not been studied in the gut. The expression of NGF, BDNF, NT-3 and TN-4 in SMCs from longitudinal and circular muscle layers of rat colon from normal and Dextran sodium sulphate (DSS)-induced colitis rats was measured by ELISA...
September 22, 2017: Physiological Research
https://www.readbyqxmd.com/read/28935635/serpinb3-is-overexpressed-in-the-liver-in-presence-of-iron-overload
#6
Cristian Turato, Patricia Kent, Giada Sebastiani, Stefania Cannito, Elisabetta Morello, Liliana Terrin, Alessandra Biasiolo, Davide Simonato, Maurizio Parola, Kostas Pantopoulos, Patrizia Pontisso
Iron overload results in cellular toxicity, tissue injury, organ fibrosis and increased risk of neoplastic transformation. SerpinB3 is a serine protease inhibitor overexpressed in the liver in oxidative stress conditions, able to induce fibrosis and increased risk of malignant transformation. Aim of the present study was to assess the effect of iron overload on SerpinB3 expression in the liver using in vivo and in vitro models.The expression of Serpinb3 was assessed in the liver of hemojuvelin knockout mice (Hjv-/-), an established model of hereditary hemochromatosis, and of wild type control mice, following dietary or pharmacological iron manipulation...
September 20, 2017: Journal of Investigative Medicine: the Official Publication of the American Federation for Clinical Research
https://www.readbyqxmd.com/read/28935001/lack-of-effect-of-murine-astrovirus-infection-on-dextran-sulfate-induced-colitis-in-nlrp3-deficient-mice
#7
Susan R Compton, Carmen J Booth, James D Macy
Murine astrovirus (MuAstV) is a recently identified, widespread infection among laboratory mice. MuAstV is found predominantly in the gastrointestinal tract of mice. Human and turkey astroviruses have been shown to disrupt tight junctions in the intestinal epithelium. The potential of MuAstV to alter research results was tested in a well-established dextran sodium sulfate (DSS)-induced colitis model in Nod-like receptor 3 (NLRP3)-deficient mice. This model offers a direct approach to determine whether MuAstV, as a component of the mouse microbiome, contributes to the issue of poor reproducibility in murine inflammatory bowel disease research...
October 1, 2017: Comparative Medicine
https://www.readbyqxmd.com/read/28935000/effect-of-early-life-gut-mucosal-compromise-on-disease-progression-in-nod-mice
#8
Katja M Bendtsen, Camilla Hf Hansen, Lukasz Krych, Karsten Buschard, Helene Farlov, Axel K Hansen
Disease expression in spontaneous nonobese diabetic (NOD) mice depends on environmental stimuli such as stress, diet, and gut microbiota composition. We evaluated a brief, early-life gut intervention in which pups were weaned to low-dose dextran sulfate sodium (DSS). We hypothesized that the mucus-reducing effect of this compound and subsequent increased host-bacterial contact would delay disease onset and decrease insulitis due to enhanced oral tolerance. However, disease incidence did not differ between groups, although median survival (time point when 50% of the mice are still alive) of the control group was 184 d compared with 205 d for DSS-treated mice...
October 1, 2017: Comparative Medicine
https://www.readbyqxmd.com/read/28934661/dextran-coated-silver-nanoparticles-chemical-sensor-for-selective-cysteine-detection
#9
Slađana Davidović, Vesna Lazić, Ivana Vukoje, Jelena Papan, S Phillip Anhrenkiel, Suzana Dimitrijević, Jovan M Nedeljković
A simple, fast and non-costly method for selective cysteine (Cys) detection, based on optical changes of silver colloids, is developed. For that purpose, stable colloids consisting of silver nanoparticles (Ag NPs) coated with polysaccharide dextran (Dex), isolated from bacterium species Leuconostoc mesenteroides T3, were prepared. The synthesized samples were thoroughly characterized including absorption and FTIR spectroscopy, as well as transmission electron microscopy and X-ray diffraction analysis. The silver colloids display high sensitivity and selectivity towards Cys detection in aqueous solutions...
September 12, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28933966/mobile-laser-indirect-ophthalmoscope-for-the-induction-of-choroidal-neovascularization-in-a-mouse-model
#10
Dov Weinberger, Elite Bor-Shavit, Tilda Barliya, Mor Dahbash, Opher Kinrot, Dan D Gaton, Yael Nisgav, Tami Livnat
PURPOSE: This study aims to evaluate and standardize the reliability of a mobile laser indirect ophthalmoscope in the induction of choroidal neovascularization (CNV) in a mouse model. MATERIALS & METHODS: A diode laser indirect ophthalmoscope was used to induce CNV in pigmented male C57BL/6J mice. Standardization of spot size and laser intensity was determined using different aspheric lenses with increasing laser intensities applied around the optic disc. Development of CNV was evaluated 1, 5, and 14 days post laser application using fluorescein angiography (FA), histology, and choroidal flat mounts stained for the endothelial marker CD31 and FITC-dextran...
September 21, 2017: Current Eye Research
https://www.readbyqxmd.com/read/28933847/preparation-and-characterization-of-mucoadhesive-nanoparticles-for-enhancing-cellular-uptake-of-coenzyme-q10
#11
Ji-Soo Lee, Ji Woon Suh, Eun Suh Kim, Hyeon Gyu Lee
The mucoadhesive nanoparticles (NPs) for oral delivery of coenzyme Q10 (CoQ10) were prepared using natural mucoadhesive polysaccharides, chitosan (CS) and dextran sulfate sodium salt (DS) in order to improve the solubility, cellular uptake, thermo- and photo-stability of CoQ10. CoQ10-loaded NPs were prepared in the range of 340-450 nm with an entrapment efficiency of 60-98%. The mucoadhesiveness and cellular uptake of NPs were evaluated by measuring the amount of mucin adsorbed on NPs and CoQ10 absorbed in Caco-2 cells, respectively...
September 21, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28933644/development-and-optimization-of-a-sensitive-pseudovirus-based-assay-for-hiv-1-neutralizing-antibodies-detection-using-a3r5-cells
#12
Qingqing Chen, Jianhui Nie, Weijin Huang, Yanmei Jiao, Lan Li, Tong Zhang, Juan Zhao, Hao Wu, Youchun Wang
Sensitive assays for HIV-1 neutralizing antibody detection are urgently needed for vaccine immunogen optimization and identification of protective immune response levels. In this study, we developed an easy-to-use HIV-1 pseudovirus neutralization assay based on a human CD4(+) lymphoblastoid cell line A3R5 by employing a high efficient pseudovirus production system. Optimal conditions for cell counts, infection time, virus dose and concentration of DEAE-dextran were tested and identified. For T-cell line-adapted tier 1 virus strains, significantly higher inhibitory efficiency was observed for both monoclonal neutralizing antibody (4 fold) and plasma (2 fold) samples in A3R5 than those in TZM-bl assay...
September 21, 2017: Human Vaccines & Immunotherapeutics
https://www.readbyqxmd.com/read/28932838/engineered-biomimetic-nanovesicles-show-intrinsic-anti-inflammatory-properties-for-the-treatment-of-inflammatory-bowel-diseases
#13
Claudia Corbo, Walter E Cromer, Roberto Molinaro, Naama E Toledano Furman, Kelly A Hartman, Enrica De Rosa, Christian Boada, Xin Wang, David C Zawieja, Marco Agostini, Francesco Salvatore, Bincy P Abraham, Ennio Tasciotti
Inflammatory bowel disease (IBD), which includes Crohn's disease and ulcerative colitis, is a chronic inflammatory condition of the gastrointestinal (GI) tract. Currently, it is treated with immunosuppressant or biologics that often induce severe adverse effects. Thus, there is an urgent clinical need for more specific treatments. To provide a valid therapeutic tool for IBD therapy, in this work we developed biomimetic nanovesicles by manipulating leukocyte membranes to exploit mechanisms of T-cell recruitment during inflammation...
September 21, 2017: Nanoscale
https://www.readbyqxmd.com/read/28932816/a-quorum-sensing-system-that-regulates-streptococcus-pneumoniae-biofilm-formation-and-surface-polysaccharide-production
#14
Roger Junges, Gabriela Salvadori, Sudhanshu Shekhar, Heidi A Åmdal, Jimstan N Periselneris, Tsute Chen, Jeremy S Brown, Fernanda C Petersen
Despite vaccines, Streptococcus pneumoniae kills more than a million people yearly. Thus, understanding how pneumococci transition from commensals to pathogens is particularly relevant. Quorum sensing regulates collective behaviors and thus represents a potential driver of commensal-to-pathogen transitions. Rgg/small hydrophobic peptide (SHP) quorum-sensing systems are widespread in streptococci, yet they remain largely uncharacterized in S. pneumoniae. Using directional transcriptome sequencing, we show that the S...
September 2017: MSphere
https://www.readbyqxmd.com/read/28932762/liposome-disruption-assay-to-examine-lytic-properties-of-biomolecules
#15
John R Jimah, Paul H Schlesinger, Niraj H Tolia
Proteins may have three dimensional structural or amino acid features that suggest a role in targeting and disrupting lipids within cell membranes. It is often necessary to experimentally investigate if these proteins and biomolecules are able to disrupt membranes in order to conclusively characterize the function of these biomolecules. Here, we describe an in vitro assay to evaluate the membrane lytic properties of proteins and biomolecules. Large unilamellar vesicles (liposomes) containing carboxyfluorescein at fluorescence-quenching concentrations are treated with the biomolecule of interest...
August 5, 2017: Bio-protocol
https://www.readbyqxmd.com/read/28931831/hyperhomocysteinemia-alters-retinal-endothelial-cells-barrier-function-and-angiogenic-potential-via-activation-of-oxidative-stress
#16
Riyaz Mohamed, Isha Sharma, Ahmed S Ibrahim, Heba Saleh, Nehal M Elsherbiny, Sadanand Fulzele, Khaled Elmasry, Sylvia B Smith, Mohamed Al-Shabrawey, Amany Tawfik
Hyperhomocysteinemia (HHcy) is associated with several human visual disorders, such as diabetic retinopathy (DR) and age-related macular degeneration (AMD). Breakdown of the blood-retinal barrier (BRB) is linked to vision loss in DR and AMD. Our previous work revealed that HHcy altered BRB in retinal endothelial cells in vivo. Here we hypothesize that homocysteine (Hcy) alters retinal endothelial cell barrier function and angiogenic potential via activation of oxidative stress. Human retinal endothelial cells (HRECs) treated with and without different concentrations of Hcy showed a reduction of tight junction protein expression, increased FITC dextran leakage, decreased transcellular electrical resistance and increased angiogenic potential...
September 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28931526/cellubrevin-vesicle-associated-membrane-protein-3-vamp-3-mediated-endocytosis-and-trafficking-regulate-platelet-functions
#17
Meenakshi Banerjee, Smita Joshi, Jinchao Zhang, Carole L Moncman, Shilpi Yadav, Beth A Bouchard, Brian Storrie, Sidney W Whiteheart
Endocytosis is key to fibrinogen (Fg) uptake, trafficking of integrins (αIIbβ3, αvβ3) and purinergic receptors (P2Y1, P2Y12), and thus for normal platelet function. However, the molecular machinery required and possible trafficking routes are still ill-defined. To further identify elements of the platelet endocytic machinery, we examined the role of a vesicle-residing, Soluble N-ethylmaleimide Factor Attachment Protein Receptor (v-SNARE) called Cellubrevin/Vesicle-Associated Membrane Protein-3 (VAMP-3) in platelet function...
September 20, 2017: Blood
https://www.readbyqxmd.com/read/28930937/administration-of-hypertonic-solutions-for-hemorrhagic-shock-a-systematic-review-and-meta-analysis-of-clinical-trials
#18
Meng-Che Wu, Tin-Yun Liao, Erica M Lee, Yueh-Sheng Chen, Wan-Ting Hsu, Meng-Tse Gabriel Lee, Po-Yang Tsou, Shyr-Chyr Chen, Chien-Chang Lee
BACKGROUND: Several clinical trials on hypertonic fluid administration have been completed, but the results have been inconclusive. The objective of this study is to summarize current evidence for treating hypovolemic patients with hypertonic solutions by performing a systematic review and meta-analysis. METHODS: Major electronic databases were searched from inception through June 2014. We included only randomized controlled trials involving hemorrhagic shock patients treated with hypertonic solutions...
September 19, 2017: Anesthesia and Analgesia
https://www.readbyqxmd.com/read/28929336/melatonin-alters-amino-acid-metabolism-and-inflammatory-responses-in-colitis-mice
#19
Gang Liu, Qian Jiang, Shuai Chen, Jun Fang, Wenkai Ren, Jie Yin, Kang Yao, Yulong Yin
Inflammatory bowel disease is a chronic inflammatory dysfunction of the gastrointestinal tract. This study explored the hypothesis that melatonin has beneficial functions in the mouse model of colitis induced by dextran sodium sulfate (DSS), with a specific focus on the expression of intestinal inflammatory cytokines and the serum levels of amino acids. The results revealed that mice with melatonin supplementation had a reduction in weight loss and disease index induced by DSS treatment. Melatonin stifled the expression of colonic IL-17 in mice with DSS-induced colitis...
September 19, 2017: Amino Acids
https://www.readbyqxmd.com/read/28929101/current-trends-in-volume-replacement-therapy-and-the-use-of-synthetic-colloids-in-small-animals-an-internet-based-survey-2016
#20
Ivayla D Yozova, Judith Howard, Nadja E Sigrist, Katja-Nicole Adamik
The use of synthetic colloids (SCs), particularly hydroxyethyl starch (HES), in people has changed in recent years following new evidence raising concerns about their efficacy and safety. Although fluid therapy guidelines for small animals are often extrapolated from human medicine, little information exists on current practice in veterinary medicine. The objective of the present study was to investigate current fluid selection, use of plasma volume expanders including SCs, and recent changes in their use in small animal practice...
2017: Frontiers in Veterinary Science
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