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https://www.readbyqxmd.com/read/28738563/urinary-volatile-fingerprint-based-on-mass-spectrometry-for-the-discrimination-of-patients-with-lung-cancer-and-controls
#1
Álvaro García Ramos, Ana Pérez Antón, Miguel Del Nogal Sánchez, José Luis Pérez Pavón, Bernardo Moreno Cordero
Profile signals of urine samples corresponding to patients with lung cancer and controls were obtained using a non-separative methodology. The method is based on the coupling of a headspace sampler, a programed temperature vaporizer and a mass spectrometer (HS-PTV-MS). With only a centrifugation step as prior sample treatment, the samples were subjected to the headspace generation process and the volatiles generated were introduced into the PTV where they were trapped in the Tenax® packed liner while the solvent was purged...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28737692/salt-reductions-in-some-foods-in-the-netherlands-monitoring-of-food-composition-and-salt-intake
#2
Elisabeth H M Temme, Marieke A H Hendriksen, Ivon E J Milder, Ido B Toxopeus, Susanne Westenbrink, Henny A M Brants, Daphne L van der A
BACKGROUND AND OBJECTIVES: High salt intake increases blood pressure and thereby the risk of chronic diseases. Food reformulation (or food product improvement) may lower the dietary intake of salt. This study describes the changes in salt contents of foods in the Dutch market over a five-year period (2011-2016) and differences in estimated salt intake over a 10-year period (2006-2015). METHODS: To assess the salt contents of foods; we obtained recent data from chemical analyses and from food labels...
July 22, 2017: Nutrients
https://www.readbyqxmd.com/read/28732927/ultrasound-promoted-dispersive-micro-solid-phase-extraction-of-trace-anti-hypertensive-drugs-from-biological-matrices-using-a-sonochemically-synthesized-conductive-polymer-nanocomposite
#3
Maryam Hemmati, Maryam Rajabi, Alireza Asghari
In this work, a rapid and efficient procedure named ultrasound meliorated dispersive micro solid-phase extraction followed by high performance liquid chromatography-ultra violet detection (US-D-μSPE-HPLC-UV) was developed for the pre-concentration of the main trace anti-hypertensive drugs in complex matrices. The basis of this procedure was a polypyrrole-sodium dodecylbenzenesulfonate/zinc oxide (PPy-DBSNa/ZnO) nanocomposite. It was readily synthesized by the impressive way of in situ sonochemical oxidative polymerization in the presence of some additives such as FeCl3 and DBSNa, ultimately leading to the effective coating of PPy on the ZnO nanoparticle cores...
November 2017: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/28727048/nutrient-composition-digestible-and-metabolizable-energy-content-and-prediction-of-energy-for-animal-protein-byproducts-in-finishing-pig-diets
#4
B J Kerr, R Jha, P E Urriola, G C Shurson
An industry survey and animal experiment were conducted to evaluate compositional variability and DE and ME content of animal protein byproducts and to generate equations to predict DE and ME content based on chemical analysis. For the 220 samples collected, the greatest concentration of CP was observed in blood meal (BM) and the least in meat and bone meal (MBM) and the greatest concentration of ether extract was in meat meal and the least in BM, with ash content greatest in MBM and least in BM, with Ca and P levels being 36...
June 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28720369/metabolic-work-up-of-patients-with-urolithiasis-indications-and-diagnostic-algorithm
#5
REVIEW
Hans-Göran Tiselius, Michel Daudon, Kay Thomas, Christian Seitz
CONTEXT: The incidence of urinary tract stone disease is increasing and the risk of recurrent stone formation is high. Appropriate therapeutic procedures with the aim of counteracting the progress of stone formation are highly desirable. Metabolic work-up is considered essential as a base for optimal design and follow-up of effective recurrence prevention. OBJECTIVE: To scrutinize the current literature with regard to principles of metabolic work-up for this heterogeneous group of patients...
February 2017: European Urology Focus
https://www.readbyqxmd.com/read/28711247/ratio-of-dietary-rumen-degradable-protein-to-rumen-undegradable-protein-affects-nitrogen-partitioning-but-does-not-affect-the-bovine-milk-proteome-produced-by-mid-lactation-holstein-dairy-cows
#6
R Tacoma, J Fields, D B Ebenstein, Y-W Lam, S L Greenwood
Little is known about the bovine milk proteome or whether it can be affected by diet. The objective of this study was to determine if the dietary rumen degradable protein (RDP):rumen undegradable protein (RUP) ratio could alter the bovine milk proteome. Six Holstein cows (parity: 2.5 ± 0.8) in mid lactation were blocked by days in milk (80 ± 43 d in milk) and milk yield (57.5 ± 6.0 kg) and randomly assigned to treatment groups. The experiment was conducted as a double-crossover design consisting of three 21-d periods...
July 12, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28709086/sensitive-detection-of-brucine-an-anti-metastatic-drug-for-hepatocellular-carcinoma-at-carbon-nanotubes-nafion-composite-based-biosensor
#7
Rutesh Savalia, Sanghamitra Chatterjee
A neoteric approach for the electrochemical analysis of brucine in biological environment has been developed. The glassy carbon electrode modified with single walled carbon nanotubes and nafion composite film is delineated for the first time to determine brucine employing square wave voltammetry. The quantification of brucine at physiological pH 7.2 manifests remarkable performance at the developed biosensor. The effect of several operational parameters has been studied in the present investigation. Under optimized conditions, a dynamic linear range from 1nM to 8µM with a high sensitivity of 340...
July 6, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28700482/beneficial-effects-of-fu-zheng-qu-zhuo-oral-liquid-combined-with-standard-integrated-therapy-in-patients-with-chronic-kidney-disease-stage-3-4-a-randomized-placebo-controlled-clinical-trial
#8
Shen Li, Xiang-Rong Rao, Xi-Wen Dai, Kun Pei, Lee Wang, Bao-Min Huo, Xiu-Juan Wang, Ling-Xin Kong, Nan-Nan Zhang, Feng-Mei Lian
BACKGROUND: The high worldwide prevalence of chronic kidney disease (CKD) is a critical health problem and the development of more effective therapies is urgently needed. We conducted a randomized, double-blinded, placebo-controlled clinical trial from October 2010 to December 2012 to assess whether Fu-Zheng-Qu-Zhuo oral liquid (FZQZ) has a beneficial effect in preventing CKD progression when added to standard integrated therapies. METHODS: Patients with CKD stage 3 to 4 from 3 hospitals in Beijing, China were enrolled...
July 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28698649/characterizing-the-scent-and-chemical-composition-of-panthera-leo-marking-fluid-using-solid-phase-microextraction-and-multidimensional-gas-chromatography-mass-spectrometry-olfactometry
#9
Simone B Soso, Jacek A Koziel
Lions (Panthera leo) use chemical signaling to indicate health, reproductive status, and territorial ownership. To date, no study has reported on both scent and composition of marking fluid (MF) from P. leo. The objectives of this study were to: 1) develop a novel method for simultaneous chemical and scent identification of lion MF in its totality (urine + MF), 2) identify characteristic odorants responsible for the overall scent of MF as perceived by human panelists, and 3) compare the existing library of known odorous compounds characterized as eliciting behaviors in animals in order to understand potential functionality in lion behavior...
July 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28695173/identification-of-symptom-clusters-in-cancer-patients-at-palliative-care-clinic
#10
Gülçin Şenel Özalp, Neşe Uysal, Gonca Oğuz, Nesteren Koçak, Şerife Karaca, Nihan Kadıoğulları
OBJECTIVE: Cancer patients often experience a large number of symptoms together. The aim of this study is to determine the symptom clusters in cancer patients at palliative care clinic. METHODS: Hundred and seventy consecutive patients were enrolled in the study. Memorial Symptom Assessment Scale was used for symptom assessment of the patients. RESULTS: The most experienced symptoms by the patients during the past week before hospitalization in palliative care clinic were lack of energy (95...
July 2017: Asia-Pacific Journal of Oncology Nursing
https://www.readbyqxmd.com/read/28683796/effectiveness-of-dapagliflozin-on-vascular-endothelial-function-and-glycemic-control-in-patients-with-early-stage-type-2-diabetes-mellitus-defence-study
#11
Fumika Shigiyama, Naoki Kumashiro, Masahiko Miyagi, Kayoko Ikehara, Eiichiro Kanda, Hiroshi Uchino, Takahisa Hirose
BACKGROUND: Recent studies reported that sodium glucose cotransporter 2 (SGLT2) inhibitors can potentially reduce the risk of cardiovascular mortality in patients with type 2 diabetes mellitus (T2DM). However, there is little or no information on the therapeutic effects of SGLT2 inhibitors on the progression of atherosclerosis. This dapagliflozin effectiveness on vascular endothelial function and glycemic control (DEFENCE) study was designed to determine the effects of dapagliflozin, a SGLT2 inhibitor, on endothelial function in patients with early-stage T2DM...
July 6, 2017: Cardiovascular Diabetology
https://www.readbyqxmd.com/read/28667496/skin-health-connected-to-the-use-of-absorbent-hygiene-products-a-review
#12
REVIEW
Johanna Karlsson Bender, Jan Faergemann, Maria Sköld
Over the past 50 years, absorbent hygiene products such as baby diapers and incontinence products have become essential features of modern day life. Through innovation and enhanced technology, their design, composition and performance have been dramatically upgraded from their early forms, and they have transformed the lives of millions of people, improving their quality of life. Skin health related to the use of absorbent hygiene products has accordingly also greatly improved. Still, the wearing of absorbent hygiene products will affect the skin, and for some users the changes in microclimate, mechanical interactions and the exposure to urine and faeces may result in irritant contact dermatitis, i...
June 30, 2017: Dermatology and Therapy
https://www.readbyqxmd.com/read/28646338/chemotherapy-induced-gastrointestinal-toxicity-is-associated-with-changes-in-serum-and-urine-metabolome-and-fecal-microbiota-in-male-sprague-dawley-rats
#13
Richard A Forsgård, Vannina G Marrachelli, Katri Korpela, Rafael Frias, Maria Carmen Collado, Riitta Korpela, Daniel Monleon, Thomas Spillmann, Pia Österlund
PURPOSE: Chemotherapy-induced gastrointestinal toxicity (CIGT) is a complex process that involves multiple pathophysiological mechanisms. We have previously shown that commonly used chemotherapeutics 5-fluorouracil, oxaliplatin, and irinotecan damage the intestinal mucosa and increase intestinal permeability to iohexol. We hypothesized that CIGT is associated with alterations in fecal microbiota and metabolome. Our aim was to characterize these changes and examine how they relate to the severity of CIGT...
June 23, 2017: Cancer Chemotherapy and Pharmacology
https://www.readbyqxmd.com/read/28638886/mechanisms-of-coix-seed-compositions-in-the-treatment-of-spleen-deficiency-and-wet-dampness-zheng
#14
Xiaochun Han, Xuming Ji, Haijun Zhao, Yanan Zhang, Guowei Liu, Yanfang Wang, Wenxiao Zhao, Shijun Wang
BACKGROUND: Coix seed has the functions of fortifying the spleen and inhibiting the dampness. However, it remains unclear which Coix seed compositions is responsible for these functions. Previous investigations have revealed that the main compositions of Coix seed are proteins, polysaccharides, oils and starches. The objectives of this study are to explore which is the most effective compositions in fortifying the spleen and examine how Coix seed works in regulating the water transport on the spleen deficiency and wet dampness (SDWD) rat model...
2017: African Journal of Traditional, Complementary, and Alternative Medicines: AJTCAM
https://www.readbyqxmd.com/read/28635280/antiadhesive-activity-and-metabolomics-analysis-of-rat-urine-after-cranberry-vaccinium-macrocarpon-aiton-administration
#15
Gregorio Peron, Anna Pellizzaro, Paola Brun, Elisabetta Schievano, Stefano Mammi, Stefania Sut, Ignazio Castagliuolo, Stefano Dall'Acqua
Cranberry (Vaccinium macrocarpon Aiton) is used to treat noncomplicated urinary tract infections (UTIs). A-type procyanidins (PAC-A) are considered the active constituents able to inhibit bacterial adhesion to the urinary epithelium. However, the role of PAC-A in UTIs is debated, because of their poor bioavailability, extensive metabolism, limited knowledge about urinary excretion, and contradictory clinical trials. The effects of 35-day cranberry supplementation (11 mg/kg PAC-A, 4 mg/kg PAC-B) were studied in healthy rats using a ultra performance liquid chromatography-mass spectrometry (UPLC-MS)-based metabolomics approach...
July 5, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28633848/novel-synthesis-of-nanocomposite-for-the-extraction-of-sildenafil-citrate-viagra-from-water-and-urine-samples-process-screening-and-optimization
#16
Arash Asfaram, Mehrorang Ghaedi, Mihir Kumar Purkait
A sensitive analytical method is investigated to concentrate and determine trace level of Sildenafil Citrate (SLC) present in water and urine samples. The method is based on a sample treatment using dispersive solid-phase micro-extraction (DSPME) with laboratory-made Mn@ CuS/ZnS nanocomposite loaded on activated carbon (Mn@ CuS/ZnS-NCs-AC) as a sorbent for the target analyte. The efficiency was enhanced by ultrasound-assisted (UA) with dispersive nanocomposite solid-phase micro-extraction (UA-DNSPME). Four significant variables affecting SLC recovery like; pH, eluent volume, sonication time and adsorbent mass were selected by the Plackett-Burman design (PBD) experiments...
September 2017: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/28632180/preview-prevention-of-diabetes-through-lifestyle-intervention-and-population-studies-in-europe-and-around-the-world-design-methods-and-baseline-participant-description-of-an-adult-cohort-enrolled-into-a-three-year-randomised-clinical-trial
#17
Mikael Fogelholm, Thomas Meinert Larsen, Margriet Westerterp-Plantenga, Ian Macdonald, J Alfredo Martinez, Nadka Boyadjieva, Sally Poppitt, Wolfgang Schlicht, Gareth Stratton, Jouko Sundvall, Tony Lam, Elli Jalo, Pia Christensen, Mathijs Drummen, Elizabeth Simpson, Santiago Navas-Carretero, Teodora Handjieva-Darlenska, Roslyn Muirhead, Marta P Silvestre, Daniela Kahlert, Laura Pastor-Sanz, Jennie Brand-Miller, Anne Raben
Type-2 diabetes (T2D) is one of the fastest growing chronic diseases worldwide. The PREVIEW project has been initiated to find the most effective lifestyle (diet and physical activity) for the prevention of T2D, in overweight and obese participants with increased risk for T2D. The study is a three-year multi-centre, 2 × 2 factorial, randomised controlled trial. The impact of a high-protein, low-glycaemic index (GI) vs. moderate protein, moderate-GI diet in combination with moderate or high-intensity physical activity on the incidence of T2D and the related clinical end-points are investigated...
June 20, 2017: Nutrients
https://www.readbyqxmd.com/read/28629683/the-relationship-between-dietary-intake-growth-and-body-composition-in-inborn-errors-of-intermediary-protein-metabolism
#18
Maureen Evans, Helen Truby, Avihu Boneh
OBJECTIVES: To examine relationships between dietary intake, growth and body composition patterns in patients with inborn errors of intermediary protein metabolism and to determine a safe protein:energy ratio (P:E ratio) associated with optimal growth outcomes. STUDY DESIGN: Retrospective longitudinal data of growth and dietary intake in patients (n = 75) with isovaleric acidemia (IVA; n = 7), methylmalonic acidemia/propionic acidemia (MMA/PA; n = 14), urea cycle defects (UCD; n = 44), classical maple syrup urine disease (MSUD; n = 10) were collected...
June 16, 2017: Journal of Pediatrics
https://www.readbyqxmd.com/read/28628622/urinary-catheter-associated-microbiota-change-in-accordance-with-treatment-and-infection-status
#19
Laetitia Bossa, Kimberly Kline, Diane McDougald, Bonsan Bonne Lee, Scott A Rice
The use of long-term catheterisation to manage insensate bladders, often associated with spinal cord injury (SCI), increases the risk of microbial colonisation and infection of the urinary tract. Urinary tract infection (UTI) is typically diagnosed and treated based on the culturing of organisms from the urine, although this approach overlooks low titer, slow growing and non-traditional pathogens. Here, we present an investigation of the urinary tract microbiome in catheterised SCI individuals, using T-RFLP and metagenomic sequencing of the microbial community...
2017: PloS One
https://www.readbyqxmd.com/read/28627269/profiling-and-identification-of-chlorogenic-acid-metabolites-in-rats-by-ultra-high-performance-liquid-chromatography-coupled-with-linear-ion-trap-orbitrap-mass-spectrometer
#20
Fei Wang, Zhanpeng Shang, Lulu Xu, Zhibin Wang, Wenjing Zhao, XiaoDan Mei, Jianqiu Lu, Jiangyu Zhang
1. Chlorogenic acids (CGAs), one kind of major bioactive constituents isolated from Flos Lonicerae Japonicae, possess many biological activities, such as antibacterial, antioxidant, and antiviral activities. In this study, we established an efficient strategy using ultra-high-performance liquid chromatography coupled with linear ion trap-Orbitrap mass spectrometry (UHPLC-LTQ-Orbitrap MS) to profile the in vivo metabolic fate of CGAs in rat urine and plasma. 2. The extract from Flos Lonicerae Japonicae was orally administrated to Sprague-Dawley (SD) rats at a dose of 1000 mg/kg body weight...
June 19, 2017: Xenobiotica; the Fate of Foreign Compounds in Biological Systems
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