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urine composition

Holly Kramer, Gina Kuffel, Krystal Thomas-White, Alan J Wolfe, Kavitha Vellanki, David J Leehey, Vinod K Bansal, Linda Brubaker, Robert Flanigan, Julia Koval, Anuradha Wadhwa, Michael J Zilliox
PURPOSE: To examine the characteristics of the midstream urine microbiome in adults with stage 3-5 non-dialysis-dependent chronic kidney disease (CKD). METHODS: Patients with non-dialysis-dependent CKD (estimated glomerular filtration rate [eGFR] < 60 ml/min/1.73 m2 ) and diuretic use were recruited from outpatient nephrology clinics. Midstream voided urine specimens were collected using the clean-catch method. The bacterial composition was determined by sequencing the hypervariable (V4) region of the bacterial 16S ribosomal RNA gene...
April 12, 2018: International Urology and Nephrology
Anirudha Anirudhasingh Gmail Com Singh, David Lee, Harrison Jeong, Christine Yu, Jiuru Li, ChenHao Fang, Praveena Sabnekar, Xiaopu Liu, Takahiro Yoshida, Nikolai Sopko, Trinity Bivalacqua
Decellularized tissues have been increasingly popular for constructing scaffolds for tissue engineering applications due to their beneficial biological compositions and mechanical properties. It is therefore natural to consider decellularized trachea for construction of tissue-engineered trachea as well as other tubular organs. A neo-urinary conduit (NUC) is such a tubular organ that works as a passage for urine removal in bladder cancer patients who need a urinary diversion after their diseased bladder is removed...
April 13, 2018: Tissue Engineering. Part A
Lyubov Chaykovska, Fabian Heunisch, Gina von Einem, Carl-Friedrich Hocher, Oleg Tsuprykov, Mira Pavkovic, Peter Sandner, Axel Kretschmer, Chang Chu, Saban Elitok, Johannes-Peter Stasch, Berthold Hocher
BACKGROUND: The use of iodine-based contrast agents entails the risk of contrast induced nephropathy (CIN). Radiocontrast agents elicit the third most common cause of nephropathy among hospitalized patients, accounting for 11-12% of cases. CIN is connected with clinically significant consequences, including increased morbidity, prolonged hospitalization, increased risk of complications, potential need for dialysis, and increased mortality rate. The number of in-hospital examinations using iodine-based contrast media has been significantly increasing over the last decade...
2018: PloS One
Brenda Eskenazi, Sookee An, Stephen A Rauch, Eric S Coker, Angelina Maphula, Muvhulawa Obida, Madelein Crause, Katherine R Kogut, Riana Bornman, Jonathan Chevrier
BACKGROUND: Although indoor residual spraying (IRS) with dichlorodiphenyltrichloroethane (DDT) and pyrethroids effectively controls malaria, it potentially increases human exposure to these insecticides. Previous studies suggest that prenatal exposure to these insecticides may impact human neurodevelopment. OBJECTIVES: We aimed to estimate the effects of maternal insecticide exposure and neurodevelopment of toddlers living in a malaria-endemic region currently using IRS...
April 6, 2018: Environmental Health Perspectives
Daniel G K Rasmussen, Tine W Hansen, Bernt J von Scholten, Signe H Nielsen, Henrik Reinhard, Hans-Henrik Parving, Martin Tepel, Morten A Karsdal, Peter K Jacobsen, Federica Genovese, Peter Rossing
OBJECTIVE: Type 2 diabetes is a common risk factor for the development of chronic kidney disease (CKD). Enhanced de novo collagen type VI (COL VI) formation has been associated with renal fibrosis and CKD. We investigated the hypothesis that PRO-C6, a product specifically generated during COL VI formation, is prognostic for adverse outcomes in patients with type 2 diabetes and microalbuminuria. RESEARCH DESIGN AND METHODS: In a prospective, observational study, we measured PRO-C6 in serum ( S -PRO-C6) and urine (U-PRO-C6) of 198 patients with type 2 diabetes and microalbuminuria without symptoms of coronary artery disease...
April 11, 2018: Diabetes Care
A L Silva, E Detmann, J Dijkstra, A M Pedroso, L H P Silva, A F Machado, F C Sousa, G B Dos Santos, M I Marcondes
The objective of this study was to evaluate the influence of different amounts of rumen-undegradable protein (RUP) on intake, N balance, performance, mammary gland development, carcass traits, and hormonal status of Holstein heifers at different physiological stages (PS). Sixteen prepubertal (PRE) heifers (initial BW = 106 ± 7.6 kg; age = 4.3 ± 0.46 mo) and 16 pubertal (PUB) heifers (initial BW = 224 ± 7.9 kg; age = 12.6 ± 0.45 mo) were used in an experiment over a period of 84 d. Four diets with increasing RUP contents (38, 44, 51, and 57% of dietary crude protein) and heifers at 2 PS (PRE or PUB) were used in a 4 × 2 factorial arrangement of treatments in a completely randomized design...
April 4, 2018: Journal of Dairy Science
Lei Zhang, Jing Zhang
By introduction of Mo metal species (molybdenum-based polyoxometalates) into the Cu-MOF as co-precursor, molybdenum carbide nanosphere (Mox C@C) was prepared via a simple calcining routine and a further etching the metallic Cu process. The obtained Mox C@C showed a unique structure where well-dispersed Mox C nanoparticles (NPs) were encapsulated in porous carbon matrix. As-fabricated novel 3D porous architecture Mox C@C nanosphere exhibited a potent and persistent electro-oxidation behavior followed by well-separated oxidation peaks (peak to peak voltage is about 350 mV) toward adenine (A) and guanine (G) by differential pulse voltammetry (DPV)...
March 29, 2018: Biosensors & Bioelectronics
David A Drew, Ronit Katz, Stephen Kritchevsky, Joachim H Ix, Michael G Shlipak, Anne B Newman, Andy Hoofnagle, Linda Fried, Mark J Sarnak, Orlando M Gutierrez
BACKGROUND: Fibroblast growth factor 23 (FGF-23) is a hormone that regulates phosphorus levels and vitamin D metabolism. Previous studies have shown FGF-23 to be a risk factor for incident end-stage renal disease; however, there are less data on the association of FGF-23 with earlier kidney-related outcomes. METHODS: Serum FGF-23 was assayed using an intact ELISA assay in 2,496 participants of the Healthy Aging and Body Composition Study, a cohort of well-functioning older adults...
April 5, 2018: American Journal of Nephrology
Courtney N Hemphill, Tryon A Wickersham, Jason E Sawyer, T M Brown-Brandl, Harvey C Freetly, Kristin E Hales
The objective of this study was to determine if feeding monensin would improve diet digestion, energy and nitrogen balance in bred heifers receiving a limit-fed corn stalk-based diet. Sixteen pregnant Meat Animal Research Center (MARC) III composite heifers were used in a 161-d completely randomized design. Heifers were randomly assigned to one of two treatments, no monensin (CON) or 150 mg/d monensin (MON), with eight heifers in each treatment group. Heifers were limit-fed a corn stalk-based diet at 100% of MEm requirements...
April 2, 2018: Journal of Animal Science
Sigrid Beer-Borst, Xhyljeta Luta, Stefanie Hayoz, Kathrin Sommerhalder, Corinna Gréa Krause, Julia Eisenblätter, Sandra Jent, Stefan Siegenthaler, Rafael Aubert, Max Haldimann, Pasquale Strazzullo
BACKGROUND: Blood pressure is a primary cardiovascular disease risk factor. Population-wide governmental strategies aim to reduce lifestyle and dietary risk factors for hypertension, one of which is an unbalanced diet with high sodium and low potassium intakes. Nutrition interventions in the workplace are considered a promising approach in encouraging health-promoting behaviors. We developed and conducted the health promoting sodium reduction trial "Healthful & Tasty: Sure!" in worksites in the German-speaking part of Switzerland from May 2015 to Nov 2016, for which we present the study protocol and baseline characteristics...
April 2, 2018: BMC Public Health
Juan Sánchez-González, Sara Odoardi, Ana María Bermejo, Pilar Bermejo-Barrera, Francesco Saverio Romolo, Antonio Moreda-Piñeiro, Sabina Strano-Rossi
Several molecularly imprinted polymers (MIPs) have been synthesized for the first time using various synthetic cannabinoids (JWH007, JWH015 and JWH098) as template molecules. Ethylene dimethacrylate (EDMA) was used as a functional monomer for all cases. Similarly, divinylbenzene (DVB) and 2,2'-azobisisobutyronitrile (AIBN) were used as cross-linker and initiator, respectively. The prepared MIPs have been fully characterized and evaluated as new selective adsorbents for micro-solid phase extraction (μ-SPE) of synthetic cannabinoids in urine...
March 26, 2018: Journal of Chromatography. A
Vasantha Jotwani, Ronit Katz, Joachim H Ix, Orlando M Gutiérrez, Michael Bennett, Chirag R Parikh, Steven R Cummings, Mark J Sarnak, Michael G Shlipak
RATIONALE & OBJECTIVE: Novel urinary biomarkers have enabled earlier detection of kidney tubular damage, but their prognostic value for adverse cardiovascular outcomes is uncertain. We hypothesized that tubular damage, measured by urine α1 -microglobulin (A1M), amino-terminal propeptide of type III procollagen (PIIINP), and neutrophil gelatinase-associated lipocalin (NGAL), would be associated with higher risks for cardiovascular events and mortality among elders. STUDY DESIGN: Case-cohort study...
March 27, 2018: American Journal of Kidney Diseases: the Official Journal of the National Kidney Foundation
Jun Peng, Huairu Tian, Qiuzheng Du, Xuanhong Hui, Hua He
Graphene oxide (GO), nanosized Fe3 O4 and zeolite imidazolate framework-8 (ZIF-8) were hybridized as a multifunctional sorbent for use in microextraction. The sorbent was characterized by SEM, TEM, XRD and FTIR. The composite is porous, has a high specific surface (> 600 m2 ·g-1 ) and is paramagnetic. The GO sheets are shown to act as carriers for the Fe3 O4 nanoparticles and ZIF-8. The composite is a viable material for the preconcentration of atorvastatin and simvastatin from urine prior to their determination by HPLC with PDA detection...
January 27, 2018: Mikrochimica Acta
Behzad Rezaei, Hamid Reza Jamei, Ali Asghar Ensafi
An aptamer-based method is described for electrochemical determination of lysozyme. A glassy carbon electrode was modified with a nanocomposite composed of multi-walled carbon nanotubes, poly(diallyl dimethyl ammonium chloride), and carbon quantum dots. The composition of the nanocomposite (MWCNT/PDDA/CQD) warrants good electrical conductivity and a high surface-to-volume ratio. The lysozyme-binding aptamers were immobilized on the nanocomposite via covalent coupling between the amino groups of the aptamer and the carboxy groups of the nanocomposite...
February 14, 2018: Mikrochimica Acta
Jing Xu, Zheng Zhang, Xiao-Mei He, Ren-Qi Wang, Dilshad Hussain, Yu-Qi Feng
The authors have immobilized nanowires made from zirconium glycerolate (ZrGly) on magnetite (Fe3 O4 ) nanoparticles by applying a solvothermal growth process using metal-glycerolate as a precursor. The structure and the dissolution-recrystallization mechanism of the resulting Fe3 O4 @ZrGly composite were investigated by attenuated total reflection-FTIR, energy-dispersive X-ray analysis, thermogravimetric analysis and solid-state cross polarization/magic angle spinning13 C NMR spectroscopy. The interaction between the zirconium glycerolate in Fe3 O4 @ZrGly and cis-diols leads to efficient adsorption of riboncleosides which then can be quantified by HPLC with UV detection...
December 8, 2017: Mikrochimica Acta
Zachariah DeFilipp, Jonathan U Peled, Shuli Li, Jasmin Mahabamunuge, Zeina Dagher, Ann E Slingerland, Candice Del Rio, Betsy Valles, Maria E Kempner, Melissa Smith, Jami Brown, Bimalangshu R Dey, Areej El-Jawahri, Steven L McAfee, Thomas R Spitzer, Karen K Ballen, Anthony D Sung, Tara E Dalton, Julia A Messina, Katja Dettmer, Gerhard Liebisch, Peter Oefner, Ying Taur, Eric G Pamer, Ernst Holler, Michael K Mansour, Marcel R M van den Brink, Elizabeth Hohmann, Robert R Jenq, Yi-Bin Chen
We hypothesized that third-party fecal microbiota transplantation (FMT) may restore intestinal microbiome diversity after allogeneic hematopoietic cell transplantation (allo-HCT). In this open-label single-group pilot study, 18 subjects were enrolled before allo-HCT and planned to receive third-party FMT capsules. FMT capsules were administered no later than 4 weeks after neutrophil engraftment, and antibiotics were not allowed within 48 hours before FMT. Five patients did not receive FMT because of the development of early acute gastrointestinal (GI) graft-versus-host disease (GVHD) before FMT (n = 3), persistent HCT-associated GI toxicity (n = 1), or patient decision (n = 1)...
April 10, 2018: Blood Advances
Loek P Smits, Ruud S Kootte, Evgeni Levin, Andrei Prodan, Susana Fuentes, Erwin G Zoetendal, Zeneng Wang, Bruce S Levison, Maartje C P Cleophas, E Marleen Kemper, Geesje M Dallinga-Thie, Albert K Groen, Leo A B Joosten, Mihai G Netea, Erik S G Stroes, Willem M de Vos, Stanley L Hazen, Max Nieuwdorp
BACKGROUND: Intestinal microbiota have been found to be linked to cardiovascular disease via conversion of the dietary compounds choline and carnitine to the atherogenic metabolite TMAO (trimethylamine-N-oxide). Specifically, a vegan diet was associated with decreased plasma TMAO levels and nearly absent TMAO production on carnitine challenge. METHODS AND RESULTS: We performed a double-blind randomized controlled pilot study in which 20 male metabolic syndrome patients were randomized to single lean vegan-donor or autologous fecal microbiota transplantation...
March 26, 2018: Journal of the American Heart Association
Alfred Haglind, Mikael Hedeland, Torbjörn Arvidsson, Curt E Pettersson
The widening application area of SFC-MS with polar analytes and water-containing samples facilitates the use of quick and simple sample preparation techniques such as "dilute and shoot" and protein precipitation. This has also introduced new polar interfering components such as alkali metal ions naturally abundant in e.g. blood plasma and urine, which have shown to be retained using screening conditions in SFC/ESI-TOF-MS and causing areas of major ion suppression. Analytes co-eluting with these clusters will have a decreased signal intensity, which might have a major effect on both quantification and identification...
March 19, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Colby J Vorland, Berdine R Martin, Connie M Weaver, Munro Peacock, Kathleen M Hill Gallant
There are limited data on phosphorus balance and the effect of dietary calcium supplements on phosphorus balance in adolescents. The purpose of this study was to determine phosphorus balance and the effect of increasing dietary calcium intake with a supplement on net phosphorus absorption and balance in healthy adolescent girls. This study utilized stored urine, fecal, and diet samples from a previously conducted study that focused on calcium balance. Eleven healthy girls ages 11 to 14 years participated in a randomized crossover study, which consisted of two 3-week periods of a controlled diet with low (817 ± 19...
March 2018: JBMR Plus
Attilio Naccarato, Rosangela Elliani, Brunella Cavaliere, Giovanni Sindona, Antonio Tagarelli
Polyamines are aliphatic amines with low molecular weight that are widely recognized as one of the most important cancer biomarkers for early diagnosis and treatment. The goal of the work herein presented is the development of a rapid and simple method for the quantification of free polyamines (i.e., putrescine, cadaverine, spermidine, spermine) and N-monoacetylated polyamines (i.e., N1 -Acetylspermidine, N8 -Acetylspermidine, and N1 -Acetylspermine) in human urine. A preliminary derivatization with propyl chloroformate combined with the use of solid phase microextraction (SPME) allowed for an easy and automatable protocol involving minimal sample handling and no consumption of organic solvents...
March 19, 2018: Journal of Chromatography. A
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