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https://www.readbyqxmd.com/read/29224163/methylation-sensitive-high-resolution-melting-ms-hrm
#1
Dianna Hussmann, Lise Lotte Hansen
Methylation-Sensitive High Resolution Melting (MS-HRM) is an in-tube, PCR-based method to detect methylation levels at specific loci of interest. A unique primer design facilitates a high sensitivity of the assays enabling detection of down to 0.1-1% methylated alleles in an unmethylated background.Primers for MS-HRM assays are designed to be complementary to the methylated allele, and a specific annealing temperature enables these primers to anneal both to the methylated and the unmethylated alleles thereby increasing the sensitivity of the assays...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29216463/application-of-double-focusing-sector-field-icp-ms-for-determination-of-ultratrace-constituents-in-samples-characterized-by-complex-composition-of-the-matrix
#2
Ilia Rodushkin, Cora Paulukat, Simon Pontér, Emma Engström, Douglas C Baxter, Dieke Sörlin, Nicola Pallavicini, Katerina Rodushkina
The performance of double focusing, sector field mass spectrometry (ICP-SFMS) for determination of analytes, including technology critical elements (TCE), at ultra-trace levels in environmental and clinical matrices was critically evaluated. Different configurations of the ICP-SFMS introduction system as well as various sample preparations, pre-concentration and matrix separation methods were employed and compared. Factors affecting detection capabilities and accuracy of data produced (instrumental sensitivity, contamination risks, purity of reagents, spectral interferences, matrix effects, analyte recovery and losses) were discussed...
December 4, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/29200368/influence-of-fasting-duration-on-body-fluid-and-hemodynamics
#3
Masanori Tsukamoto, Takashi Hitosugi, Takeshi Yokoyama
Fasting before general anesthesia aims to reduce the volume and acidity of stomach contents, which reduces the risk of regurgitation and aspiration. Prolonged fasting for many hours prior to surgery could lead to unstable hemodynamics, however. Therefore, preoperative oral intake of clear fluids 2 hours prior to surgery is recommended to decrease dehydration without an increase in aspiration risk. In this study, we investigated the body fluid composition and hemodynamics of patients undergoing general anesthesia as the first case of the day versus the second subsequent case...
2017: Anesthesia Progress
https://www.readbyqxmd.com/read/29191357/patient-specific-evolution-of-renal-function-in-chronic-heart-failure-patients-dynamically-predicts-clinical-outcome-in-the-bio-shift-study
#4
Milos Brankovic, K Martijn Akkerhuis, Nick van Boven, Sharda Anroedh, Alina Constantinescu, Kadir Caliskan, Olivier Manintveld, Jan Hein Cornel, Sara Baart, Dimitris Rizopoulos, Hans Hillege, Eric Boersma, Victor Umans, Isabella Kardys
Renal dysfunction is an important component of chronic heart failure (CHF), but its single assessment does not sufficiently reflect clinically silent progression of CHF prior to adverse clinical outcome. Therefore, we aimed to investigate temporal evolutions of glomerular and tubular markers in 263 stable patients with CHF, and to determine if their patient-specific evolutions during this clinically silent period can dynamically predict clinical outcome. We determined the risk of clinical outcome (composite endpoint of Heart Failure hospitalization, cardiac death, Left Ventricular Assist Device placement, and heart transplantation) in relation to marker levels, slopes and areas under their trajectories...
November 28, 2017: Kidney International
https://www.readbyqxmd.com/read/29178032/lithium-increases-ammonium-excretion-leading-to-altered-urinary-acid-base-buffer-composition
#5
Francesco Trepiccione, Claudia Altobelli, Giovambattista Capasso, Birgitte Mønster Christensen, Sebastian Frische
Previous reports identify a voltage dependent distal renal tubular acidosis (dRTA) secondary to lithium (Li+) salt administration. This was based on the inability of Li+-treated patients to increase the urine-blood (U-B) pCO2 when challenged with NaHCO3 and, the ability of sodium neutral phosphate or Na2SO4 administration to restore U-B pCO2 in experimental animal models. The underlying mechanisms for the Li+-induced dRTA are still unknown. To address this point, a 7 days time course of the urinary acid-base parameters was investigated in rats challenged with LiCl, LiCitrate, NaCl, or NaCitrate...
November 24, 2017: Journal of Nephrology
https://www.readbyqxmd.com/read/29162796/deconvoluting-interrelationships-between-concentrations-and-chemical-shifts-in-urine-provides-a-powerful-analysis-tool
#6
Panteleimon G Takis, Hartmut Schäfer, Manfred Spraul, Claudio Luchinat
The NMR chemical shifts of a substance in a complex mixture strongly depend on the composition of the mixture itself, as many weak interactions occur that are hardly predictable. Chemical shift variability is the major obstacle to automatically assigning, and subsequently quantitating, metabolite signals in body fluids, particularly urine. Here we demonstrate that the chemical shifts of signals in urine are actually predictable. This is achieved by constructing ca. 4000 artificial mixtures where the concentrations of 52 most abundant urine metabolites-including 11 inorganic ions-are varied, to sparsely but efficiently populate an N-dimensional concentration matrix...
November 21, 2017: Nature Communications
https://www.readbyqxmd.com/read/29158619/the-effect-of-mineral-based-alkaline-water-on-hydration-status-and-the-metabolic-response-to-short-term-anaerobic-exercise
#7
Jakub Chycki, Tomasz Zając, Adam Maszczyk, Anna Kurylas
Previously it was demonstrated that mineralization and alkalization properties of mineral water are important factors influencing acid-base balance and hydration in athletes. The purpose of this study was to investigate the effects of drinking different types of water on urine pH, specific urine gravity, and post-exercise lactate utilization in response to strenuous exercise. Thirty-six male soccer players were divided into three intervention groups, consuming around 4.0 l/day of different types of water for 7 days: HM (n=12; highly mineralized water), LM (n=12; low mineralized water), and CON (n=12; table water)...
September 2017: Biology of Sport
https://www.readbyqxmd.com/read/29155503/relatives-in-silent-kidney-disease-screening-risks-study-a-chinese-cohort-study
#8
Philip Kam-Tao Li, Jack Kit-Chung Ng, Yuk Lun Cheng, Tze Hoi Kwan, Chi Bon Leung, Miu Fong Lau, Koon Shing Choi, Samuel Ka-Shun Fung, Yiu Wing Ho, Siu Ka Mak, Sydney Chi-Wai Tang, Kin Shing Wong, David Yong, Sing Leung Lui
AIM: Family members of patients with end-stage renal disease (ESRD) have higher risk for chronic kidney disease (CKD). Limited study has examined the risk of developing CKD in relatives of patients in earlier stages of CKD. METHODS: From January 2008 to June 2009, the Hong Kong Society of Nephrology studied first-degree relatives of stage 1-5 CKD patients from 11 local hospitals. A total of 844 relatives of 466 index CKD patients (stages 1-2: 29.6%; stage 3: 16...
December 2017: Nephrology
https://www.readbyqxmd.com/read/29150995/efficacy-and-safety-of-extracranial-vein-angioplasty-in-multiple-sclerosis-a-randomized-clinical-trial
#9
Paolo Zamboni, Luigi Tesio, Stefania Galimberti, Luca Massacesi, Fabrizio Salvi, Roberto D'Alessandro, Patrizia Cenni, Roberto Galeotti, Donato Papini, Roberto D'Amico, Silvana Simi, Maria Grazia Valsecchi, Graziella Filippini
Importance: Chronic cerebrospinal venous insufficiency (CCSVI) is characterized by restricted venous outflow from the brain and spinal cord. Whether this condition is associated with multiple sclerosis (MS) and whether venous percutaneous transluminal angioplasty (PTA) is beneficial in persons with MS and CCSVI is controversial. Objective: To determine the efficacy and safety of venous PTA in patients with MS and CCSVI. Design, Setting, and Participants: We analyzed 177 patients with relapsing-remitting MS; 62 were ineligible, including 47 (26...
November 18, 2017: JAMA Neurology
https://www.readbyqxmd.com/read/29150907/sensitivity-of-doping-biomarkers-after-administration-of-a-single-dose-testosterone-gel
#10
Jenny Mullen, Annica Börjesson, Oscar Hopcraft, Jenny J Schulze, Magnus Ericsson, Anders Rane, Mikael Lehtihet, Lena Ekström
Micro-doping with testosterone (T) is challenging to detect with the current doping tests. Today, the methods available to detect T are longitudinally monitoring of urine biomarkers in the Athlete Biological Passport (ABP) and measuring the isotopic composition of excreted biomarkers to distinguish the origin of the molecule. In this study, we investigated the detectability of a single dose of 100 mg T gel in 8 healthy male subjects. We also studied which biomarkers were most sensitive to T gel administration, including blood biomarkers...
November 18, 2017: Drug Testing and Analysis
https://www.readbyqxmd.com/read/29150416/-bicarbonate-from-physiology-to-treatment-for-all-clinicians
#11
Julie Beaume, Antoine Braconnier, Thibault Dolley-Hitze, Jean-Philippe Bertocchio
Acid-base regulation is essential to maintain homeostasis in humans. Carbonic acid/bicarbonate (H2CO3/HCO3(-)) couple is the most predominant extracellular buffer to keep plasma pH within a physiological range. The ability to (re)generate such a buffer is a key milestone that necessitates to understand a precise physiology of both renal tubule and digestive tract. Here, we first reviewed renal and digestive cycles of bicarbonate in physiology. We also reviewed pathological findings where acid-base disequilibrium is involved and nutritional and/or alkali therapy could be necessary...
November 14, 2017: Néphrologie & Thérapeutique
https://www.readbyqxmd.com/read/29137703/fabrication-of-a-novel-aptasensor-based-on-three-dimensional-reduced-graphene-oxide-polyaniline-gold-nanoparticle-composite-as-a-novel-platform-for-high-sensitive-and-specific-cocaine-detection
#12
Pegah Hashemi, Hasan Bagheri, Abbas Afkhami, Yalda Hosseinzadeh Ardakani, Tayyebeh Madrakian
In the present research, we have developed a novel label free aptasensor based on screen printed carbon electrode (SPCE) modified with three-dimensional magnetic reduced graphene oxide(3D-MRGO)/polyaniline(PA)/gold nanoparticle(AuNP) nanocomposite for impedimetric determination of cocaine. To achieve this goal, a specific thiolated cocaine aptamer was immobilized onto the surface of synthesized nanocomposite. The signaling mechanism of the proposed aptasensor was based on increase in the [Fe(CN)6]3-/4- charge transfer resistance (RCT) as an electrochemical probe in the presence of target analyte...
December 15, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/29136792/comparative-performances-of-a-bare-graphite-polyurethane-composite-electrode-unmodified-and-modified-with-graphene-and-carbon-nanotubes-in-the-electrochemical-determination-of-escitalopram
#13
Marina Baccarin, Priscila Cervini, Eder Tadeu Gomes Cavalheiro
A bare composite graphite-polyurethane electrode (EGPU) and two other modified with graphene (EGPU-GR) and functionalized multi-walled carbon nanotubes (EGPU-CNTs) were prepared and compared regarding their voltammetric response to escitalopran (EST). The modifiers were characterized by Raman spectroscopy and the resulting electrode materials by contact angle measurements with a hydrophilicity character in the ascending order for the composites: GPU > GPU-GR > GPU-CNTs and scanning electron microscopy (SEM)...
February 1, 2018: Talanta
https://www.readbyqxmd.com/read/29127057/quantitative-analysis-of-clonidine-and-ephedrine-by-a-microfluidic-system-on-chip-electromembrane-extraction-followed-by-high-performance-liquid-chromatography
#14
Mahroo Baharfar, Yadollah Yamini, Shahram Seidi, Monireh Karami
In this work, a microfluidic device was developed for on-chip electromembrane extraction of trace amounts of ephedrine (EPH) and clonidine (CLO) in human urine and plasma samples followed by HPLC-UV analysis. Two polymethylmethacrylate plates were used as substrates and a microchannel was carved in each plate. The microchannel channel on the underneath plate provided the flow pass of the sample solution and the one on the upper plate dedicated to a compartment for the stagnant acceptor phase. A piece of polypropylene sheet was impregnated by an organic solvent and mounted between the two parts of the chip device...
November 2, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29124388/measuring-the-impact-of-olive-pomace-enriched-biscuits-on-the-gut-microbiota-and-its-metabolic-activity-in-mildly-hypercholesterolaemic-subjects
#15
Lorenza Conterno, Francesca Martinelli, Matteo Tamburini, Francesca Fava, Andrea Mancini, Maddalena Sordo, Massimo Pindo, Stefan Martens, Domenico Masuero, Urska Vrhovsek, Claudia Dal Lago, Gabriele Ferrario, Mario Morandini, Kieran Tuohy
PURPOSE: Olive pomace is a major waste product of olive oil production but remains rich in polyphenols and fibres. We measured the potential of an olive pomace-enriched biscuit formulation delivering 17.1 ± 4.01 mg/100 g of hydroxytyrosol and its derivatives, to modulate the composition and metabolic activity of the human gut microbiota. METHODS: In a double-blind, controlled parallel dietary intervention 62 otherwise healthy hypercholesterolemic (total plasma cholesterol 180-240 mg/dl) subjects were randomly assigned to eat 90 g of olive pomace-enriched biscuit (olive-enriched product, OEP) or an isoenergetic control (CTRL) for 8 weeks...
November 9, 2017: European Journal of Nutrition
https://www.readbyqxmd.com/read/29102059/on-chip-pulsed-electromembrane-extraction-as-a-new-concept-for-analysis-of-biological-fluids-in-a-small-device
#16
Monireh Karami, Yadollah Yamini, Yousef Abdossalami Asl, Maryam Rezazadeh
In the present work, an on-chip pulsed electromembrane extraction technique followed by HPLC-UV was developed for the analysis of codeine, naloxone and naltrexone as model analytes in biological fluids. The chip consisted of two channels for the introduction of the donor and acceptor phases. The channels were carved in two poly (methyl methacrylate) plates and a porous polypropylene membrane, which is impregnated by an organic solvent separating the two channels. Two platinum electrodes were mounted on the bottom of these channels and a pulsed electrical voltage was applied as an electrical driving force for the migration of ionized analytes from the sample solution through the porous sheet membrane into the acceptor phase...
October 23, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/29100363/alterations-in-expressed-prostate-secretion-urine-psa-n-glycosylation-discriminate-prostate-cancer-from-benign-prostate-hyperplasia
#17
Gaozhen Jia, Zhenyang Dong, Chenxia Sun, Fuping Wen, Haifeng Wang, Huaizu Guo, Xu Gao, Chuanliang Xu, Chuanliang Xu, Chenghua Yang, Yinghao Sun
The prostate specific antigen (PSA) test is widely used for early diagnosis of prostate cancer (PCa). However, its limited sensitivity has led to over-diagnosis and over-treatment of PCa. Glycosylation alteration is a common phenomenon in cancer development. Different PSA glycan subforms have been proposed as diagnostic markers to better differentiate PCa from benign prostate hyperplasia (BPH). In this study, we purified PSA from expressed prostate secretions (EPS)-urine samples from 32 BPH and 30 PCa patients and provided detailed PSA glycan profiles in Chinese population...
September 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/29097482/urine-anion-gap-to-predict-urine-ammonium-and-related-outcomes-in-kidney-disease
#18
Kalani L Raphael, Sarah Gilligan, Joachim H Ix
BACKGROUND AND OBJECTIVES: Low urine ammonium excretion is associated with ESRD in CKD. Few laboratories measure urine ammonium, limiting clinical application. We determined correlations between urine ammonium, the standard urine anion gap, and a modified urine anion gap that includes sulfate and phosphate and compared risks of ESRD or death between these ammonium estimates and directly measured ammonium. DESIGN, SETTING, PARTICIPANTS, & MEASUREMENTS: We measured ammonium, sodium, potassium, chloride, phosphate, and sulfate from baseline 24-hour urine collections in 1044 African-American Study of Kidney Disease and Hypertension participants...
November 2, 2017: Clinical Journal of the American Society of Nephrology: CJASN
https://www.readbyqxmd.com/read/29097438/whole-grain-rich-diet-reduces-body-weight-and-systemic-low-grade-inflammation-without-inducing-major-changes-of-the-gut-microbiome-a-randomised-cross-over-trial
#19
Henrik Munch Roager, Josef K Vogt, Mette Kristensen, Lea Benedicte S Hansen, Sabine Ibrügger, Rasmus B Mærkedahl, Martin Iain Bahl, Mads Vendelbo Lind, Rikke L Nielsen, Hanne Frøkiær, Rikke Juul Gøbel, Rikard Landberg, Alastair B Ross, Susanne Brix, Jesper Holck, Anne S Meyer, Morten H Sparholt, Anders F Christensen, Vera Carvalho, Jens Juul Holst, Jüri Johannes Rumessen, Allan Linneberg, Thomas Sicheritz-Pontén, Marlene D Dalgaard, Andreas Blennow, Henrik Lauritz Frandsen, Silas Villas-Bôas, Karsten Kristiansen, Henrik Vestergaard, Torben Hansen, Claus T Ekstrøm, Christian Ritz, Henrik Bjørn Nielsen, Oluf Borbye Pedersen, Ramneek Gupta, Lotte Lauritzen, Tine Rask Licht
OBJECTIVE: To investigate whether a whole grain diet alters the gut microbiome and insulin sensitivity, as well as biomarkers of metabolic health and gut functionality. DESIGN: 60 Danish adults at risk of developing metabolic syndrome were included in a randomised cross-over trial with two 8-week dietary intervention periods comprising whole grain diet and refined grain diet, separated by a washout period of ≥6 weeks. The response to the interventions on the gut microbiome composition and insulin sensitivity as well on measures of glucose and lipid metabolism, gut functionality, inflammatory markers, anthropometry and urine metabolomics were assessed...
November 1, 2017: Gut
https://www.readbyqxmd.com/read/29097390/differential-impact-of-serial-measurement-of-nonplatelet-thromboxane-generation-on-long-term-outcome-after-cardiac-surgery
#20
Nikolaos Kakouros, Tyler J Gluckman, John V Conte, Thomas S Kickler, Katherine Laws, Bruce A Barton, Jeffrey J Rade
BACKGROUND: Systemic thromboxane generation, not suppressible by standard aspirin therapy and likely arising from nonplatelet sources, increases the risk of atherothrombosis and death in patients with cardiovascular disease. In the RIGOR (Reduction in Graft Occlusion Rates) study, greater nonplatelet thromboxane generation occurred early compared with late after coronary artery bypass graft surgery, although only the latter correlated with graft failure. We hypothesize that a similar differential association exists between nonplatelet thromboxane generation and long-term clinical outcome...
November 2, 2017: Journal of the American Heart Association
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