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simultaneous drug detection

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https://www.readbyqxmd.com/read/28549217/surface-enhanced-raman-scattering-active-gold-nanoparticles-with-enzyme-mimicking-activities-for-measuring-glucose-and-lactate-in-living-tissues
#1
Yihui Hu, Hanjun Cheng, Xiaozhi Zhao, Jiangjiexing Wu, Faheem Muhammad, Shichao Lin, Jian He, Liqi Zhou, Chengping Zhang, Yu Deng, Peng Wang, Zhengyang Zhou, Shuming Nie, Hui Wei
Gold nanoparticles (AuNPs) with simultaneous plasmonic and biocatalytic properties provide a promising approach to developing versatile bioassays. However, the combination of AuNPs' intrinsic enzyme mimicking properties with their surface-enhanced Ra-man scattering (SERS) activities has yet to be explored. Here we designed a peroxidase mimicking nanozyme by in situ growing AuNPs into a highly porous and thermally stable metal-organic framework (MOF) called MIL-101. The obtained AuNPs@MIL-101 nanozymes acted as the peroxidase mimics to oxidize Raman inactive reporter leucomalachite green (LMG) into the active malachite green (MG) with hydrogen peroxide, and simultaneously as the SERS substrates to enhance the Raman signals of the as-produced MG...
May 26, 2017: ACS Nano
https://www.readbyqxmd.com/read/28547184/combination-of-magnetic-dispersive-micro-solid-phase-extraction-and-supramolecular-solvent-based-microextraction-followed-by-high-performance-liquid-chromatography-for-determination-of-trace-amounts-of-cholesterol-lowering-drugs-in-complicated-matrices
#2
Somayeh Arghavani-Beydokhti, Maryam Rajabi, Alireza Asghari
A novel, efficient, rapid, simple, sensitive, selective, and environmentally friendly method termed magnetic dispersive micro solid-phase extraction combined with supramolecular solvent-based microextraction (Mdμ-SPE-SSME) followed by high-performance liquid chromatography (HPLC) with UV detection is introduced for the simultaneous microextraction of cholesterol-lowering drugs in complicated matrices. In the first microextraction procedure, using layered double hydroxide (LDH)-coated Fe3O4 magnetic nanoparticles, an efficient sample cleanup is simply and rapidly provided without the need for time-consuming centrifugation and elution steps...
May 26, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28545929/a-simple-hplc-uv-method-for-quantification-of-enzalutamide-and-its-active-metabolite-n-desmethyl-enzalutamide-in-patients-with-metastatic-castration-resistant-prostate-cancer
#3
Alicja Puszkiel, Alain Plé, Olivier Huillard, Gaëlle Noé, Constance Thibault, Stéphane Oudard, François Goldwasser, Michel Vidal, Jérome Alexandre, Benoit Blanchet
Enzalutamide is currently approved for the treatment of patients with metastatic castration-resistant prostate cancer (mCRPC). To date, a single liquid chromatographic-tandem mass spectroscopy method is available to measure plasma enzalutamide concentrations in mCRPC patients. In this work, an accurate and sensitive HPLC-UV method has been developed for the simultaneous determination of enzalutamide and its active metabolite, N-desmethyl enzalutamide in plasma from mCRPC patients. Before precipitation of proteins with acetonitrile, samples were spiked with nilutamide (internal standard)...
April 5, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28545032/endoplasmic-reticulum-stress-mediated-membrane-expression-of-crt-erp57-induces-immunogenic-apoptosis-in-drug-resistant-endometrial-cancer-cells
#4
Qin Xu, Chuanben Chen, An Lin, Yunqing Xie
OBJECTIVE: To investigate the role of endoplasmic reticulum (ER) stress-mediated CRT/ERp57 complex expression underlying the mechanism of resistance to doxorubicin (DOX) in endometrial carcinoma (EC) in vivo and in vitro. METHODS: The expression of CRT, ERp57, p-PERK, eIF2α, p-eIF2α in EC patients and EC cells was detected by Western blots and by immunofluorescence assay. MTT assay was used to determine the LC50 of EC cells to DOX and cell viability. Apoptosis was assayed using flow cytometer...
May 8, 2017: Oncotarget
https://www.readbyqxmd.com/read/28544885/overcoming-interference-with-the-detection-of-a-stable-isotopically-labeled-microtracer-in-the-evaluation-of-beclabuvir-absolute-bioavailability-using-a-concomitant-microtracer-approach
#5
Hao Jiang, Craig Titsch, Jianing Zeng, Barry Jones, Philip Joyce, Yash Gandhi, Wesley Turley, Richard Burrell, Anne F Aubry, Mark E Arnold
The oral absolute bioavailability of beclabuvir in healthy subjects was determined using a microdose (100μg) of the stable isotopically labeled tracer via intravenous (IV) infusion started after oral dosing of beclabuvir (150mg). To simultaneously analyze the concentrations of the IV microtracer ([(13)C6]beclabuvir) and beclabuvir in plasma samples, a liquid chromatography-triple quadrupole mass spectrometry (LC-MS/MS) method was initially developed. Surprisingly beclabuvir significantly interfered with the IV microtracer detection when using the selected reaction monitoring (SRM) in the assay...
May 17, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28544466/simultaneous-imaging-of-endogenous-survivin-mrna-and-on-demand-drug-release-in-live-cells-by-using-a-mesoporous-silica-nanoquencher
#6
Peiyan Yuan, Xin Mao, Kok Chan Chong, Jiaqi Fu, Sijun Pan, Shuizhu Wu, Changmin Yu, Shao Q Yao
The design of multifunctional drug delivery systems capable of simultaneous target detection, imaging, and therapeutics in live mammalian cells is critical for biomedical research. In this study, by using mesoporous silica nanoparticles (MSNs) chemically modified with a small-molecule dark quencher, followed by sequential drug encapsulation, MSN capping with a dye-labeled antisense oligonucleotide, and bioorthogonal surface modification with cell-penetrating poly(disulfide)s, the authors have successfully developed the first mesoporous silica nanoquencher (qMSN), characterized by high drug-loading and endocytosis-independent cell uptake, which is able to quantitatively image endogenous survivin mRNA and release the loaded drug in a manner that depends on the survivin expression level in tumor cells...
May 24, 2017: Small
https://www.readbyqxmd.com/read/28532129/-131-i-traced-plga-lipid-nanoparticles-as-drug-delivery-carriers-for-the-targeted-chemotherapeutic-treatment-of-melanoma
#7
Haiyan Wang, Weizhong Sheng
Herein, folic acid (FA) conjugated Poly(d,l-lactide-co-glycolide) (PLGA)-lipid composites (FA-PL) were developed as nanocarriers for the targeted delivery of insoluble anti-cancer drug paclitaxel (PTX), resulting FA-PLP nanoparticles. Furthermore, (131)I, as a radioactive tracer, was used to label FA-PLP nanoparticles (FA-PLP-(131)I) to evaluate their cell uptake activity, in vivo blood circulation, and biodistribution. The FA-PLP-(131)I nanoparticles had a spherical morphology with great stability, a narrow size distribution (165...
December 2017: Nanoscale Research Letters
https://www.readbyqxmd.com/read/28524783/quantitative-analysis-of-veterinary-drugs-in-bovine-muscle-and-milk-by-liquid-chromatography-quadrupole-time-of-flight-mass-spectrometry
#8
Shizuka Saito-Shida, Takatoshi Sakai, Satoru Nemoto, Hiroshi Akiyama
A simple and reliable multiresidue method for quantitative determination of veterinary drugs in bovine muscle and milk using liquid chromatography quadrupole time-of-flight mass spectrometry (LC-QTOF-MS) was developed. Critical MS parameters such as capillary voltage, cone voltage, collision energy, desolvation gas temperature, and extraction mass window were carefully optimised to obtain the best possible sensitivity. Analytical samples were prepared using extraction with acetonitrile and hexane in the presence of anhydrous sodium sulphate and acetic acid, followed by ODS cartridge clean-up...
May 19, 2017: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
https://www.readbyqxmd.com/read/28521190/simultaneous-determination-of-bisphenol-a-and-estrogens-in-hair-samples-by-liquid-chromatography-electrospray-tandem-mass-spectrometry
#9
Chaelin Lee, Chong Hyeak Kim, Sunghwan Kim, Sung-Hee Cho
Bisphenol A (BPA), an endocrine disrupter, is widely used to make chemicals for polycarbonate, plastics, beverage containers, epoxy resins, and cash register receipts. BPA is one of the known xenoestrogens, which have weak estrogenic activity and cause obesity, diabetes, breast cancer, and reproductive disorders. Even though the concentration level of metabolomes in hair is usually lower than that in urine and blood, there are several reasons why we chose to use hair samples. First, the sampling procedure of hairs is simple...
May 11, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28503249/hierarchical-models-for-multiple-rare-outcomes-using-massive-observational-healthcare-databases
#10
Trevor R Shaddox, Patrick B Ryan, Martijn J Schuemie, David Madigan, Marc A Suchard
Clinical trials often lack power to identify rare adverse drug events (ADEs) and therefore cannot address the threat rare ADEs pose, motivating the need for new ADE detection techniques. Emerging national patient claims and electronic health record databases have inspired post-approval early detection methods like the Bayesian self-controlled case series (BSCCS) regression model. Existing BSCCS models do not account for multiple outcomes, where pathology may be shared across different ADEs. We integrate a pathology hierarchy into the BSCCS model by developing a novel informative hierarchical prior linking outcome-specific effects...
August 2016: Statistical Analysis and Data Mining
https://www.readbyqxmd.com/read/28498911/different-chromatographic-methods-for-simultaneous-determination-of-mefenamic-acid-and-two-of-its-toxic-impurities
#11
Martha M Morcoss, Nada S Abdelwahab, Nouruddin W Ali, Mohammed T Elsaady
Two sensitive, accurate and precise chromatographic methods mentioned as TLC-densitometric method and RP-HPLC-DAD method, were developed and validated for the simultaneous determination of mefenamic acid (MEF) and its two toxic impurities, benzoic acid (BA) and 2,3-dimethylaniline (DMA). In the proposed TLC-densitometric method a developing system consisting of chloroform:acetone:acetic acid:ammonia solution(70:30:2:2, v/v/v/v) was used, TLC aluminum plates 60 F254 was used as a stationary phase and the separated bands were UV-scanned at 225 nm...
May 11, 2017: Journal of Chromatographic Science
https://www.readbyqxmd.com/read/28497121/implementation-of-isavuconazole-in-a-fluorescence-based-high-performance-liquid-chromatography-kit-allowing-simultaneous-detection-of-all-four-currently-licensed-mold-active-triazoles
#12
René Jørgensen, Siri Rytcher Andersen, Karen Marie Thyssen Astvad, Maiken Cavling Arendrup
Isavuconazole (ISZ) is a newly available broad-spectrum triazole agent recently approved for the treatment of both invasive aspergillosis and mucormycosis. The aim of this study was to develop a simple and reliable method for therapeutic drug monitoring (TDM) of ISZ in human plasma samples. The method involves using a kit from ChromSystems intended for TDM of itraconazole (ITZ), posaconazole (PSZ), and voriconazole (VRZ) in serum/plasma for sample preparation and high-performance liquid chromatography, using fluorescence detection with emission and excitation wavelengths set to 261 and 366 nm, respectively...
May 2017: MSphere
https://www.readbyqxmd.com/read/28493020/development-and-validation-of-an-analytical-method-based-on-liquid-chromatography-tandem-mass-spectrometry-detection-for-the-simultaneous-determination-of-13-relevant-wastewater-derived-contaminants-in-lettuce
#13
Nicola Montemurro, Cristina Postigo, Antonio Lonigro, Sandra Perez, Damià Barceló
We present an analytical method developed and validated to study the potential uptake of 13 selected drugs (ten pharmaceuticals, one illicit drug, and two transformation products) into lettuce plants from contaminated water and soil. Some of the selected drugs (i.e., cocaine, methadone, cis-diltiazem, valsartan, and valsartan acid), which are commonly present in treated wastewater, were investigated for the first time in plant tissues. The method is based on ultrasonic solvent extraction with acetonitrile-methanol (1:1, v/v) and subsequent automated extract cleanup and analysis by means of online solid-phase extraction-liquid chromatography-tandem mass spectrometry...
May 11, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28490048/simultaneous-determination-of-imatinib-dasatinib-and-nilotinib-by-liquid-chromatography-tandem-mass-spectrometry-and-its-application-to-therapeutic-drug-monitoring
#14
Aneta Wojnicz, Beatriz Colom-Fernández, Juan L Steegmann, Cecilia Muñoz-Calleja, Francisco Abad-Santos, Ana Ruiz-Nuño
BACKGROUND: Imatinib, dasatinib, and nilotinib are tyrosine kinase inhibitors (TKIs) used as first-line treatment of chronic myeloid leukemia. Therapeutic drug monitoring is important to achieve treatment efficacy in the case of imatinib and nilotinib, and to control toxicity in the case of dasatinib. New high-sensitivity methods to monitor those drugs are needed, especially for dasatinib. Thus, a simple method to determine plasma levels of imatinib, dasatinib, and nilotinib for application in clinical practice was developed...
June 2017: Therapeutic Drug Monitoring
https://www.readbyqxmd.com/read/28490047/development-and-validation-of-an-lc-ms-ms-method-for-the-simultaneous-quantification-of-abiraterone-enzalutamide-and-their-major-metabolites-in-human-plasma
#15
Merel van Nuland, M J X Hillebrand, H Rosing, J H M Schellens, J H Beijnen
BACKGROUND: Abiraterone acetate and enzalutamide are 2 novel drugs for the treatment of metastatic castration-resistant prostate cancer. The metabolism of these drugs is extensive. Major metabolites are N-desmethyl enzalutamide, enzalutamide carboxylic acid, abiraterone N-oxide sulfate, and abiraterone sulfate; of which N-desmethyl enzalutamide is reported to possess antiandrogen capacities. A liquid chromatography-tandem mass spectrometry method for simultaneous quantification of abiraterone, enzalutamide, and the main metabolites has been developed and validated to support therapeutic drug monitoring...
June 2017: Therapeutic Drug Monitoring
https://www.readbyqxmd.com/read/28487913/recent-developments-in-protease-activity-assays-and-sensors
#16
REVIEW
Irvine Lian Hao Ong, Kun-Lin Yang
Proteases play a pivotal role in regulating important physiological processes from food digestion to blood clotting. They are also important biomarkers for many diseases such as cancers. The importance of proteases has led to extensive efforts in the screening of proteases and their inhibitors as potential drug molecules. For example, human immunodeficiency virus (HIV) patients have been treated with HIV-1 protease inhibitors to prolong the life expectancy of patients. Such a close relationship between diseases and proteases provides a strong motivation for developing sensitive, selective, and robust protease assays and sensors, which can be exploited to discover new proteases and inhibitors...
May 10, 2017: Analyst
https://www.readbyqxmd.com/read/28483280/stacking-cyclodextrin-microchip-electrokinetic-chromatographic-determination-of-gabapentinoid-drugs-in-pharmaceutical-and-biological-matrices
#17
Abdallah M Zeid, Noritada Kaji, Jenny Jeehan M Nasr, Fathalla F Belal, Yoshinobu Baba, Mohamed I Walash
A facile, rapid, and highly sensitive microchip-based electrokinetic chromatographic method was developed for the simultaneous analysis of two gabapentinoid drugs, gabapentin (GPN) and pregabalin (PGN). Both drugs were first reacted with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) via nucleophilic substitution reactions to yield highly fluorescent products with λex/em 470/540nm. Analyses of both fluorescently labeled compounds were achieved within 200s in a poly(methyl methacrylate) (PMMA) microchip with a 30mm separation channel...
April 26, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28477383/screening-free-radical-scavengers-in-xiexin-tang-by-hplc-abts-dad-q-tof-ms
#18
Yu-Qing Wang, Shu-Jiao Li, Guo Zhuang, Rong-Hui Geng, Xu Jiang
Xiexin Tang (XXT) is a traditional Chinese medicine (TCM) that has been used in herbal clinics for more than 1800 years. Many studies have shown that XXT has therapeutic effects on patients with arteriosclerosis (AS) owing to its antioxidant activity. However, there is little information about the relationship between the chemical composition of XXT and its antioxidant activity. In this study, the HPLC-ABTS-DAD-Q-TOF/MS method, which can simultaneously identify individual components and rapidly screen for antioxidant compounds, was used to screen and identify antioxidant components in XXT...
May 6, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28474802/rapid-and-sensitive-detection-of-ugt1a1-polymorphisms-associated-with-irinotecan-toxicity-by-a-novel-dna-microarray
#19
Ryouichi Tsunedomi, Shoichi Hazama, Naoko Okayama, Masaaki Oka, Hiroaki Nagano
Recent developments in the field of human genomics have greatly enhanced the potential for precision and personalized medicine. We have developed a novel DNA microarray, using a 3-mm square chip coated with diamond-like carbon to enhance the signal-to-background ratio, for use as an in vitro diagnostic tool in precision medicine. To verify the genotyping effectiveness of this newly developed DNA microarray we examined UDP-glucuronosyltransferase 1A1 (UGT1A1) polymorphisms in DNA extracted from patients with metastatic colorectal cancer...
May 5, 2017: Cancer Science
https://www.readbyqxmd.com/read/28463706/thc-and-cbd-in-blood-samples-and-seizures-in-norway-does-cbd-affect-thc-induced-impairment-in-apprehended-subjects
#20
Stine Marie Havig, Gudrun Høiseth, Maren Cecilie Strand, Ritva Anneli Karinen, Gerd-Wenche Brochmann, Dag Helge Strand, Liliana Bachs, Vigdis Vindenes
BACKGROUND AND AIMS: Several publications have suggested increasing cannabis potency over the last decade, which, together with lower amounts of cannabidiol (CBD), could contribute to an increase in adverse effects after cannabis smoking. Naturalistic studies on tetrahydrocannabinol (THC) and CBD in blood samples are, however, missing. This study aimed to investigate the relationship between THC- and CBD concentrations in blood samples among cannabis users, and to compare cannabinoid concentrations with the outcome of a clinical test of impairment (CTI) and between traffic accidents and non-accident driving under the influence of drugs (DUID)-cases...
April 15, 2017: Forensic Science International
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