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miR-204

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https://www.readbyqxmd.com/read/27907930/microrna-profiling-in-clear-cell-renal-cell-carcinoma-tissues-potentially-links-tumorigenesis-and-recurrence-with-obesity
#1
X Shu, M A Hildebrandt, J Gu, N M Tannir, S F Matin, J A Karam, C G Wood, X Wu
BACKGROUND: Twenty to 40% localised RCC patients may experience recurrence after curative surgery. Limited miRNA predictors have been identified for ccRCC recurrence. METHODS: Through a multi-phase study design, we analysed miRNAs in tissues obtained from 203 ccRCC patients. Paired t-test was used for tumour-normal comparisons and Cox regression model was performed to compute hazard ratios (HRs) and corresponding 95% CIs. RESULTS: A 17-miRNA signature was identified that can concordantly classify >95% of tumour/adjacent normal samples...
December 1, 2016: British Journal of Cancer
https://www.readbyqxmd.com/read/27807800/establishment-of-a-mirna-mrna-regulatory-network-in-metastatic-renal-cell-carcinoma-and-screening-of-potential-therapeutic-targets
#2
Jie Zhu, Xin Ma, Yu Zhang, Dong Ni, Qing Ai, Hongzhao Li, Xu Zhang
This study aimed to screen effective diagnosis or treatment biomarkers for renal cell carcinoma, especially for metastatic renal cell carcinoma (mRCC) based on microRNA (miRNA) and messenger RNA (mRNA) genechip, and their regulatory network. The differential expressions of miRNAs and mRNAs were examined by miRNA and mRNA gene-chip analyses, respectively, in patients with either localized renal cell carcinoma (lRCC) or mRCC, and a miRNA-mRNA regulatory network was established. Subsequently, the regulation of selected mRNAs by miRNAs was validated by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and dual-luciferase reporter gene assay...
November 2, 2016: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/27789576/the-role-and-regulation-of-rab40b-tks5-complex-during-invadopodia-formation-and-cancer-cell-invasion
#3
Abitha Jacob, Erik Linklater, Brian A Bayless, Traci Lyons, Rytis Prekeris
Invadopodia formation and extracellular matrix degradation are key events during cancer cell invasion, yet little is known about mechanisms mediating these processes. Here, we report that Rab40b plays a key role in mediating invadopodia function during breast cancer cell invasion. We also identify Tks5 (also known as SH3PXD2A), a known Src kinase substrate, as a new Rab40b effector protein and show that Tks5 functions as a tether that mediates Rab40b-dependent targeting of transport vesicles containing MMP2 and MMP9 to the extending invadopodia...
December 1, 2016: Journal of Cell Science
https://www.readbyqxmd.com/read/27753902/os-02-02-microrna-204-and-sirtuin1-regulate-vascular-endoplasmic-reticulum-stress-and-endothelium-dependent-vasorelaxation
#4
Modar Kassan, Ajit Vikram, Qiuxia Li, Young-Rae Kim, Santosh Kumar, Julia Jacob, Irani Kaikobad
OBJECTIVE: Endoplasmic reticulum (ER) stress leads to vascular endothelial dysfunction in hypertension. The role of microRNAs in vascular ER stress is not fully understood. Here we examined the role of vascular microRNA-204 (miR-204) in inducing endothelial ER stress and dysfunction by governing the expression of the SIRTUIN1 (SIRT1) lysine deacetylase. DESIGN AND METHOD: Human Umbilical Vein cells (HUVECs) were treated with tunicamycin or transfected with miR-204 Mimic or siRNA SIRT1...
September 2016: Journal of Hypertension
https://www.readbyqxmd.com/read/27748572/mir-204-5p-targeting-sirt1-regulates-hepatocellular-carcinoma-progression
#5
Guangbin Jiang, Li Wen, Hongmei Zheng, Zhiyuan Jian, Weiping Deng
Hepatocellular carcinoma (HCC) is the most common type of cancer, which presents rapid tumor growth, drug resistance, and metastasis. Recently, microRNAs are shown to be involved in the cell biological processes in HCC, but the underlying molecular mechanisms remain unclear. This study aimed to investigate the cellular function and molecular mechanism of miR-204-5p in HCC. SIRT1 mRNA and miR-204-5p were examined by real-time reverse transcription polymerase chain reaction. SIRT1 protein levels were measured by Western blotting...
October 2016: Cell Biochemistry and Function
https://www.readbyqxmd.com/read/27739496/the-microrna-expression-profile-of-mouse-m%C3%A3-ller-glia-in-vivo-and-in-vitro
#6
Stefanie G Wohl, Thomas A Reh
The profile of miRNAs in mature glia is not well characterized, and most studies have been done in cultured glia. In order to identify the miRNAs in adult and young (postnatal day 11/12) Müller glia of the neural retina, we isolated the Müller glia from Rlbp-CreER: Stop(f/f)-tdTomato mice by means of fluorescent activated cell sorting and analyzed their miRNAs using NanoStrings Technologies(®). In freshly isolated adult Müller glia, we identified 7 miRNAs with high expression levels in the glia, but very low levels in the retinal neurons...
October 14, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27734898/microrna-mir-204-and-mir-1236-inhibit-hepatitis-b-virus-replication-via-two-different-mechanisms
#7
Jyun-Yuan Huang, Hung-Lin Chen, Chiaho Shih
Hepatitis B virus (HBV) is a major human pathogen. In this study, we found that miR-204 and miR-1236 were down-regulated in HBV-producing cells, and each could suppress HBV replication. Using a bioinformatic approach and a reporter assay, we identified miR-1236, which can reduce HBV replication and protein production by directly targeting at HBV specific mRNA. In contrast, miR-204 was identified by a microarray approach, and had no effect on HBV RNA and protein production. Surprisingly, miR-204 could inhibit HBV pregenomic RNA encapsidation and capsid assembly...
October 13, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27733811/proinflammatory-cytokines-decrease-the-expression-of-genes-critical-for-rpe-function
#8
R Krishnan Kutty, William Samuel, Kaifa Boyce, Aswini Cherukuri, Todd Duncan, Cynthia Jaworski, Chandrasekharam N Nagineni, T Michael Redmond
PURPOSE: Proinflammatory cytokines interferon gamma (IFN-γ), tumor necrosis factor alpha (TNF-α), and interleukin-1 beta (IL-1β) secreted by infiltrating lymphocytes or macrophages may play a role in triggering RPE dysfunction associated with age-related macular degeneration (AMD). Binding of these proinflammatory cytokines to their specific receptors residing on the RPE cell surface can activate signaling pathways that, in turn, may dysregulate cellular gene expression. The purpose of the present study was to investigate whether IFN-γ, TNF-α, and IL-1β have an adverse effect on the expression of genes essential for RPE function, employing the RPE cell line ARPE-19 as a model system...
2016: Molecular Vision
https://www.readbyqxmd.com/read/27703260/dual-targeting-of-angpt1-and-tgfbr2-genes-by-mir-204-controls-angiogenesis-in-breast-cancer
#9
Ali Flores-Pérez, Laurence A Marchat, Sergio Rodríguez-Cuevas, Verónica Bautista-Piña, Alfredo Hidalgo-Miranda, Elena Aréchaga Ocampo, Mónica Sierra Martínez, Carlos Palma-Flores, Miguel A Fonseca-Sánchez, Horacio Astudillo-de la Vega, Erika Ruíz-García, Juan Antonio González-Barrios, Carlos Pérez-Plasencia, María L Streber, César López-Camarillo
Deregulated expression of microRNAs has been associated with angiogenesis. Studying the miRNome of locally advanced breast tumors we unsuspectedly found a dramatically repression of miR-204, a small non-coding RNA with no previous involvement in tumor angiogenesis. Downregulation of miR-204 was confirmed in an independent cohort of patients and breast cancer cell lines. Gain-of-function analysis indicates that ectopic expression of miR-204 impairs cell proliferation, anchorage-independent growth, migration, invasion, and the formation of 3D capillary networks in vitro...
October 5, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27695046/role-of-dicer1-dependent-factors-in-the-paracrine-regulation-of-epididymal-gene-expression
#10
Olivia Jerczynski, Nicolas Lacroix-Pépin, Eric Boilard, Ezequiel Calvo, Agathe Bernet, Michel A Fortier, Ida Björkgren, Petra Sipilä, Clémence Belleannée
Dicer1 is an endoribonuclease involved in the biogenesis of functional molecules such as microRNAs (miRNAs) and endogenous small interfering RNAs (endo-siRNAs). These small non-coding RNAs are important regulators of post-transcriptional gene expression and participate in the control of male fertility. With the knowledge that 1) Dicer1-dependent factors are required for proper sperm maturation in the epididymis, and that 2) miRNAs are potent mediators of intercellular communication in most biological systems, we investigated the role of Dicer1-dependent factors produced by the proximal epididymis (initial segment/caput)- including miRNAs- on the regulation of epididymal gene expression in the distal epididymis regions (i...
2016: PloS One
https://www.readbyqxmd.com/read/27686228/the-uca1-mir-204-sirt1-axis-modulates-docetaxel-sensitivity-of-prostate-cancer-cells
#11
Xu Wang, Bo Yang, Baojing Ma
PURPOSE: In this study, we firstly investigated the regulative effect of miR-204 on Sirt1 expression in prostate cancer cells. Then, we examined whether miR-204 downregulation in the cells is a result of UCA1 upregulation. In addition, the regulative effect of UCA1/miR-204/Sirt1 axis on docetaxel sensitivity of prostate cancer cells was studied. METHODS: QRT-PCR was performed to detect UCA1, miR-204 and Sirt1 mRNA expression. Western blot assay was performed to assess Sirt1, P-gp and caspase-3 expression...
September 29, 2016: Cancer Chemotherapy and Pharmacology
https://www.readbyqxmd.com/read/27682490/-the-expression-of-serum-mirnas-in-pneumoconiosis-complicated-with-pulmonary-tuberculosis-patients
#12
Y T Liu, Q Zeng, S X Li, D Y Yang, W Y Xing, L M Feng, M Zhang
Objective: To inquiry the changes of serum miRNAs expression in pneumoconiosis complicated with pulmonary tuberculosis patients. Methods: The study subjects (all subjects are male) were divided into three groups: Ⅰstage pneumoconiosis (38 cases) 、Pneumoconiosis complicated with pulmonary tuberculosis (12 cases) and healthy control (40cases) . The first two groups come from Tianjin worker's sanatorium and healthy control group came from a Hospital Health Screening Center. RT-qPCR was used to detect the expression levels of miR-21, miR-200c, miR-16, miR-204, miR-206, miR-155, let-7g, miR-30b, miR-192, miR-29a in serum...
July 20, 2016: Chinese Journal of Industrial Hygiene and Occupational Diseases
https://www.readbyqxmd.com/read/27682205/impact-of-endurance-exercise-training-on-adipocyte-microrna-expression-in-overweight-men
#13
Thomas Tsiloulis, Joshua Pike, David Powell, Fernando J Rossello, Benedict J Canny, Ruth C R Meex, Matthew J Watt
Adipocytes are major regulators of metabolism and endurance exercise training improves adipocyte function; however, the molecular mechanisms that regulate chronic adaptive responses remain unresolved. microRNAs (miRNAs) influence adipocyte differentiation and metabolism. Accordingly, we aimed to determine whether adipocyte miRNA expression is responsive to exercise training and to identify exercise-responsive miRNAs that influence adipocyte metabolism. Next generation sequencing was used to profile miRNA expression of adipocytes that were isolated from subcutaneous abdominal (ABD) and gluteofemoral (GF) adipose tissue of overweight men before and after 6 wk of endurance exercise training...
September 28, 2016: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/27667646/lncrna-uca1-enhances-cell-proliferation-through-functioning-as-a-cerna-of-sox4-in-esophageal-cancer
#14
Changjie Jiao, Zhiming Song, Jianming Chen, Jing Zhong, Weibin Cai, Suke Tian, Songlin Chen, Yunfeng Yi, Yun Xiao
Esophageal cancer (EC) is one of the most common gastrointestinal cancers, which leads to the sixth ranking of cancer-related death. Long non-coding RNAs (lncRNAs) play pivotal roles in many biological processes. lncRNA human urothelial carcinoma associated 1 (UCA1) is significantly upregulated and functions as an important oncogene in many types of human cancers. However, the role of UCA1 in EC and its underlying mechanism remains unclear. In the present study, we demonstrated that UCA1 was significantly upregulated in EC tissues and associated with poor prognosis...
September 22, 2016: Oncology Reports
https://www.readbyqxmd.com/read/27643248/os-02-02-microrna-204-and-sirtuin1-regulate-vascular-endoplasmic-reticulum-stress-and-endothelium-dependent-vasorelaxation
#15
Modar Kassan, Ajit Vikram, Qiuxia Li, Young-Rae Kim, Santosh Kumar, Julia Jacob, Irani Kaikobad
OBJECTIVE: Endoplasmic reticulum (ER) stress leads to vascular endothelial dysfunction in hypertension. The role of microRNAs in vascular ER stress is not fully understood. Here we examined the role of vascular microRNA-204 (miR-204) in inducing endothelial ER stress and dysfunction by governing the expression of the SIRTUIN1 (SIRT1) lysine deacetylase. DESIGN AND METHOD: Human Umbilical Vein cells (HUVECs) were treated with tunicamycin or transfected with miR-204 Mimic or siRNA SIRT1...
September 2016: Journal of Hypertension
https://www.readbyqxmd.com/read/27588402/mir-204-suppresses-the-development-and-progression-of-human-glioblastoma-by-targeting-atf2
#16
Shiwei Song, Abul Fajol, Xiankun Tu, Baogang Ren, Songsheng Shi
In human cancers, miRNAs are important regulators of multiple cellular processes, and aberrant miRNA expression has been observed, and their alterations contribute to multiple cancer development and progression. Till now, little has been known about the role of miR-204 in human glioblastoma (GBM). In the present study, we used in-vitro assays to investigate the mechanisms of miR-204 in GBM cell lines and 60 cases of GBM tissues. Here, we found that miR-204 expression is downregulated in both GBM cell lines A172, U87 and U251 cells and GBM tissues as compared with NHA cells and normal tissues (all p<0...
August 31, 2016: Oncotarget
https://www.readbyqxmd.com/read/27586459/vascular-microrna-204-is-remotely-governed-by-the-microbiome-and-impairs-endothelium-dependent-vasorelaxation-by-downregulating-sirtuin1
#17
Ajit Vikram, Young-Rae Kim, Santosh Kumar, Qiuxia Li, Modar Kassan, Julia S Jacobs, Kaikobad Irani
Gut microbiota promotes atherosclerosis, and vascular endothelial dysfunction, signalled by impaired endothelium-dependent vasorelaxation, is an early marker of atherosclerosis. Here we show that vascular microRNA-204 (miR-204) expression is remotely regulated by the microbiome, and impairs endothelial function by targeting the Sirtuin1 lysine deacetylase (Sirt1). MiR-204 is downregulated, while Sirt1 is upregulated, in aortas of germ-free mice. Suppression of gut microbiome with broad-spectrum antibiotics decreases miR-204, increases Sirt1 and bioavailable vascular nitric oxide, and improves endothelium-dependent vasorelaxation in mouse aortas...
September 2, 2016: Nature Communications
https://www.readbyqxmd.com/read/27571956/weighted-gene-co-expression-network-analysis-of-colorectal-cancer-liver-metastasis-genome-sequencing-data-and-screening-of-anti-metastasis-drugs
#18
Bo Gao, Qin Shao, Hani Choudhry, Victoria Marcus, Kung Dong, Jiannis Ragoussis, Zu-Hua Gao
Approximately 9% of cancer-related deaths are caused by colorectal cancer (CRC). CRC patients are prone to liver metastasis, which is the most important cause for the high CRC mortality rate. Understanding the molecular mechanism of CRC liver metastasis could help us to find novel targets for the effective treatment of this deadly disease. Using weighted gene co-expression network analysis on the sequencing data of CRC with and with metastasis, we identified 5 colorectal cancer liver metastasis related modules which were labeled as brown, blue, grey, yellow and turquoise...
September 2016: International Journal of Oncology
https://www.readbyqxmd.com/read/27565418/the-expression-of-microrna-574-3p-as-a-predictor-of-postoperative-outcome-in-patients-with-esophageal-squamous-cell-carcinoma
#19
Tomoyuki Okumura, Hirohumi Kojima, Takeshi Miwa, Shinichi Sekine, Isaya Hashimoto, Shozo Hojo, Takuya Nagata, Yutaka Shimada
BACKGROUND: Despite advances in radical esophagectomies and adjuvant therapy, the postoperative prognosis in esophageal squamous cell carcinoma (ESCC) patients remains poor. The aim of this study was to identify a molecular signature to predict postoperative favorable outcomes in patients with ESCC. METHODS: As a training data set, total RNA was extracted from formalin-fixed paraffin-embedded samples of surgically removed specimens from 19 ESCC patients who underwent curative esophagectomy...
2016: World Journal of Surgical Oncology
https://www.readbyqxmd.com/read/27519795/tumor-suppressor-mirna-204-5p-promotes-apoptosis-by-targeting-bcl2-in-prostate-cancer-cells
#20
Yi-Chia Lin, Ji-Fan Lin, Te-Fu Tsai, Kuang-Yu Chou, Hung-En Chen, Thomas I-Sheng Hwang
BACKGROUND: Prostate cancer (PCa) is a leading cause of cancer-related death in men, which emphasizes the need for novel therapeutic approaches. Targeting microRNA (miRNA) has been considered as a therapeutic strategy against cancers. Human miR-204-5p potentially targeting BCL2 has been reported to be downregulated in various cancers. We hypothesized that miR-204-5p overexpression induces cancer cell apoptosis by repressing BCL2 expression. METHODS: A vector harboring mature miR-204-5p was constructed and delivered into human PCa cells...
August 9, 2016: Asian Journal of Surgery
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