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https://www.readbyqxmd.com/read/27911378/iridium-oxide-reduced-graphene-oxide-nanohybrid-thin-film-modified-screen-printed-electrodes-as-disposable-electrochemical-paper-microfluidic-ph-sensors
#1
Jiang Yang, Tae-Joon Kwak, Xiaodong Zhang, Robert McClain, Woo-Jin Chang, Sundaram Gunasekaran
A facile, controllable, inexpensive and green electrochemical synthesis of IrO2-graphene nanohybrid thin films is developed to fabricate an easy-to-use integrated paper microfluidic electrochemical pH sensor for resource-limited settings. Taking advantages from both pH meters and strips, the pH sensing platform is composed of hydrophobic barrier-patterned paper micropad (µPAD) using polydimethylsiloxane (PDMS), screen-printed electrode (SPE) modified with IrO2-graphene films and molded acrylonitrile butadiene styrene (ABS) plastic holder...
November 22, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27904390/ascorbate-oxidase-based-amperometric-biosensor-for-l-ascorbic-acid-determination-in-beverages
#2
Gábor Csiffáry, Péter Fűtő, Nóra Adányi, Attila Kiss
A novel biosensor for l-ascorbic acid determination in different beverages was elaborated. The ascorbate oxidase enzyme (AAO) from Cucurbita sp., EC 1.10.3.3, was immobilized on a screen-printed carbon electrode with poly(ethylene glycol) (400) diglycidyl ether (PEGDGE) as a crosslinking agent. The standards and samples were measured first with a blank electrode. An inert protein, bovine serum albumin (BSA), was immobilized on the surface of this electrode with PEGDGE. The BSA mass was equivalent to the mass of 10 U of AAO enzyme immobilized on the electrodes (0...
March 2016: Food Technology and Biotechnology
https://www.readbyqxmd.com/read/27886072/peroxynitrite-sensor-based-on-a-screen-printed-carbon-electrode-modified-with-a-poly-2-6-dihydroxynaphthalene-film
#3
Ioana Silvia Hosu, Diana Constantinescu-Aruxandei, Maria-Luiza Jecu, Florin Oancea, Mihaela Badea Doni
For the first time the electropolymerization of 2,6-dihydroxynaphthalene (2,6-DHN) on a screen printed carbon electrode (SPCE) was investigated and evaluated for peroxynitrite (PON) detection. Cyclic voltammetry was used to electrodeposit the poly(2,6-DHN) on the carbon electrode surface. The surface morphology and structure of poly(2,6-DHN) film were investigated by SEM and FTIR analysis, and the electrochemical features by cyclic voltammetry. The poly(2,6-DHN)/SPCE sensor showed excellent electrocatalytic activity for PON oxidation in alkaline solutions at very low potentials (0-100 mV vs...
November 23, 2016: Sensors
https://www.readbyqxmd.com/read/27883132/microfluidic-pdms-on-paper-pop-devices
#4
Jin-Wen Shangguan, Yu Liu, Jian-Bin Pan, Bi-Yi Xu, Jing-Juan Xu, Hong-Yuan Chen
In this paper, we propose a generalized concept of microfluidic polydimethylsiloxane (PDMS) on paper (POP) devices, which combines well the merits of paper chips and PDMS chips. First, we optimized the conditions for accurate PDMS spatial patterning on paper, based on screen printing and a high temperature enabled superfast curing technique, which enables PDMS patterning to an accuracy of tens of microns in less than ten seconds. This, in turn, makes it available for seamless, reversible and reliable integration of the resulting paper layer with other PDMS channel structures...
November 24, 2016: Lab on a Chip
https://www.readbyqxmd.com/read/27881933/new-bis-azobenzocrown-s-with-dodecylmethylmalonyl-linkers-as-ionophores-for-sodium-selective-potentiometric-sensors
#5
Elżbieta Luboch, Maciej Jeszke, Mirosław Szarmach, Natalia Łukasik
Novel biscrowns 1 and 2 were synthesized from 13-membered azobenzocrown ethers containing bromoalkylenoxy chains in para position relative to the azo group. The synthesized diester molecules are dodecylmethylmalonic acid derivatives differing by the linker length. The synthesized compounds have the potential of being used as sodium ionophores in ion-selective electrodes. They were characterized and used as ionophores in classic and miniature, solid contact (screen-printed and glassy carbon) membrane ion-selective electrodes...
2016: Journal of Inclusion Phenomena and Macrocyclic Chemistry
https://www.readbyqxmd.com/read/27879639/disposable-amperometric-immunosensor-for-the-determination-of-human-p53-protein-in-cell-lysates-using-magnetic-micro-carriers
#6
María Pedrero, F Javier Manuel de Villena, Cristina Muñoz-San Martín, Susana Campuzano, María Garranzo-Asensio, Rodrigo Barderas, José M Pingarrón
An amperometric magnetoimmunosensor for the determination of human p53 protein is described in this work using a sandwich configuration involving the covalent immobilization of a specific capture antibody onto activated carboxylic-modified magnetic beads (HOOC-MBs) and incubation of the modified MBs with a mixture of the target protein and horseradish peroxidase-labeled antibody (HRP-anti-p53). The resulting modified MBs are captured by a magnet placed under the surface of a disposable carbon screen-printed electrode (SPCE) and the amperometric responses are measured at -0...
November 21, 2016: Biosensors
https://www.readbyqxmd.com/read/27865959/ultrasensitive-cardiac-troponin-i-antibody-based-nanohybrid-sensor-for-rapid-detection-of-human-heart-attack
#7
Deepika Bhatnagar, Inderpreet Kaur, Ashok Kumar
An ultrasensitive cardiac troponin I antibody conjugated with graphene quantum dots (GQD) and polyamidoamine (PAMAM) nanohybrid modified gold electrode based sensor was developed for the rapid detection of heart attack (myocardial infarction) in human. Screen printed gold (Au) electrode was decorated with 4-aminothiophenol for amine functionalization of the Au surface. These amino groups were further coupled with carboxyl functionalities of GQD with EDC-NHS reaction. In order to enhance the sensitivity of the sensor, PAMAM dendrimer was successively embedded on GQD through carbodiimide coupling to provide ultra-high surface area for antibody immobilization...
November 16, 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27856164/paper-based-maskless-enzymatic-sensor-for-glucose-determination-combining-ink-and-wire-electrodes
#8
O Amor-Gutiérrez, E Costa Rama, A Costa-García, M T Fernández-Abedul
In this work we have developed an amperometric enzymatic biosensor in a paper-based platform with a mixed electrode configuration: carbon ink for the working electrode (WE) and metal wires (from a low-cost standard electronic connection) for reference (RE) and auxiliary electrodes (AE). A hydrophobic wax-defined paper area was impregnated with diluted carbon ink. Three gold-plated pins of the standard connection are employed, one for connecting the WE and the other two acting as RE and AE. The standard connection works as a clip in order to support the paper in between...
November 6, 2016: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27844462/analytical-description-of-degradation-relaxation-transformations-in-nanoinhomogeneous-spinel-ceramics
#9
O Shpotyuk, M Brunner, I Hadzaman, V Balitska, H Klym
Mathematical models of degradation-relaxation kinetics are considered for jammed thick-film systems composed of screen-printed spinel Cu0.1Ni0.1Co1.6Mn1.2O4 and conductive Ag or Ag-Pd alloys. Structurally intrinsic nanoinhomogeneous ceramics due to Ag and Ag-Pd diffusing agents embedded in a spinel phase environment are shown to define governing kinetics of thermally induced degradation under 170 °C obeying an obvious non-exponential behavior in a negative relative resistance drift. The characteristic stretched-to-compressed exponential crossover is detected for degradation-relaxation kinetics in thick-film systems with conductive contacts made of Ag-Pd and Ag alloys...
December 2016: Nanoscale Research Letters
https://www.readbyqxmd.com/read/27836615/facile-synthesis-of-prussian-blue-nanocubes-silver-nanowires-network-as-a-water-based-ink-for-the-direct-screen-printed-flexible-biosensor-chips
#10
Pengqi Yang, Jingmeng Peng, Zhenyu Chu, Danfeng Jiang, Wanqin Jin
The large-scale fabrication of nanocomposite based biosensors is always a challenge in the technology commercialization from laboratory to industry. In order to address this issue, we have designed a facile chemical method of fabricated nanocomposite ink applied to the screen-printed biosensor chip. This ink can be derived in the water through the in-situ growth of Prussian blue nanocubes (PBNCs) on the silver nanowires (AgNWs) to construct a composite nanostructure by a facile chemical method. Then a miniature flexible biosensor chip was screen-printed by using the prepared nanocomposite ink...
October 8, 2016: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27836596/arm-microcontroller-based-portable-nitrite-electrochemical-analyzer-using-cytochrome-c-reductase-biofunctionalized-onto-screen-printed-carbon-electrode
#11
Paulraj Santharaman, Krishna Arun Venkatesh, Kanagavel Vairamani, Alby Robson Benjamin, Niroj K Sethy, Kalpana Bhargava, Chandran Karunakaran
Nitrite (NO2(-)) supplementation limits hypoxia-induced oxidative stress and activates the alternate NO pathway which may partially account for the nitrite-mediated cardioprotection. So, sensitive and selective biosensors with point-of-care devices need to be explored to detect the physiological nitrite level due to its important role in human pathophysiology. In this work, cytochrome c reductase (CcR) biofunctionalized self assembled monolayer (SAM) functionalized on gold nanoparticles (GNPs) in polypyrrole (PPy) nanocomposite onto the screen printed carbon electrode (SPCE) was investigated as a biosensor for the detection of nitrite based on its electrochemical and catalytic properties...
October 19, 2016: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27834910/influence-of-pzt-coating-thickness-and-electrical-pole-alignment-on-microresonator-properties
#12
Giedrius Janusas, Sigita Ponelyte, Alfredas Brunius, Asta Guobiene, Andrius Vilkauskas, Arvydas Palevicius
With increasing technical requirements in the design of microresonators, the development of new techniques for lightweight, simple, and inexpensive components becomes relevant. Lead zirconate titanate (PZT) is a powerful tool in the formation of these components, allowing a self-actuation or self-sensing capability. Different fabrication methods lead to the variation of the properties of the device itself. This research paper covers the fabrication of a novel PZT film and the investigations of its chemical, surface, and dynamic properties when film thickness is varied...
November 10, 2016: Sensors
https://www.readbyqxmd.com/read/27829554/ultrasensitive-electrochemical-aptasensor-based-on-sandwich-architecture-for-selective-label-free-detection-of-colorectal-cancer-ct26-cells
#13
Ayemeh Bagheri Hashkavayi, Jahan Bakhsh Raoof, Reza Ojani, Saeid Kavoosian
Colorectal cancer is one of the most common cancers in the world and has no effective treatment. Therefore, development of new methods for early diagnosis is instantly required. Biological recognition probes such as synthetic receptor and aptamer is one of the candidate recognition layers to detect important biomolecules. In this work, an electrochemical aptasensor was developed by fabricating an aptamer-cell-aptamer sandwich architecture on an SBA-15-3-aminopropyltriethoxysilane (SBA-15-pr-NH2) and Au nanoparticles (AuNPs) modified graphite screen printed electrode (GSPE) surface for the selective, label-free detection of CT26 cancer cells...
October 19, 2016: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27827963/electrochemical-affinity-biosensors-based-on-disposable-screen-printed-electrodes-for-detection-of-food-allergens
#14
REVIEW
Alina Vasilescu, Gilvanda Nunes, Akhtar Hayat, Usman Latif, Jean-Louis Marty
Food allergens are proteins from nuts and tree nuts, fish, shellfish, wheat, soy, eggs or milk which trigger severe adverse reactions in the human body, involving IgE-type antibodies. Sensitive detection of allergens in a large variety of food matrices has become increasingly important considering the emergence of functional foods and new food manufacturing technologies. For example, proteins such as casein from milk or lysozyme and ovalbumin from eggs are sometimes used as fining agents in the wine industry...
November 5, 2016: Sensors
https://www.readbyqxmd.com/read/27827833/a-stretchable-radio-frequency-strain-sensor-using-screen-printing-technology
#15
Heijun Jeong, Sungjoon Lim
In this paper, we propose a stretchable radio-frequency (RF) strain sensor fabricated with screen printing technology. The RF sensor is designed using a half-wavelength patch that resonates at 3.7 GHz. The resonant frequency is determined by the length of the patch, and it therefore changes when the patch is stretched. Polydimethylsiloxane (PDMS) is used to create the substrate, because of its stretchable and screen-printable surface. In addition, Dupont PE872 (Dupont, NC, American) silver conductive ink is used to create the stretchable conductive patterns...
November 2, 2016: Sensors
https://www.readbyqxmd.com/read/27808302/electrochemical-characterization-of-the-pyranose-2-oxidase-variant-n593c-shows-a-complete-loss-of-the-oxidase-function-with-full-preservation-of-substrate-dehydrogenase-activity
#16
Dagmar Brugger, Leander Sützl, Kawah Zahma, Dietmar Haltrich, Clemens K Peterbauer, Leonard Stoica
This study presents the first electrochemical characterization of the pyranose oxidase (POx) variant N593C (herein called POx-C), which is considered a promising candidate for future glucose-sensing applications. The resulting cyclic voltammograms obtained in the presence of various concentrations of glucose and mediator (1,4-benzoquinone, BQ), as well as the control experiments by addition of catalase, support the conclusion of a complete suppression of the oxidase function and oxygen reactivity at POx-C. Additionally, these electrochemical experiments demonstrate, contrary to previous biochemical studies, that POx-C has a fully retained enzymatic activity towards glucose...
November 30, 2016: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/27801797/electrochemical-behavior-and-determination-of-chlorogenic-acid-based-on-multi-walled-carbon-nanotubes-modified-screen-printed-electrode
#17
Xiaoyan Ma, Hongqiao Yang, Huabin Xiong, Xiaofen Li, Jinting Gao, Yuntao Gao
In this paper, the multi-walled carbon nanotubes modified screen-printed electrode (MWCNTs/SPE) was prepared and the MWCNTs/SPE was employed for the electrochemical determination of the antioxidant substance chlorogenic acids (CGAs). A pair of well-defined redox peaks of CGA was observed at the MWCNTs/SPE in 0.10 mol/L acetic acid-sodium acetate buffer (pH 6.2) and the electrode process was adsorption-controlled. Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) methods for the determination of CGA were proposed based on the MWCNTs/SPE...
October 27, 2016: Sensors
https://www.readbyqxmd.com/read/27792199/nonenzymatic-glucose-sensor-based-on-in-situ-reduction-of-ni-nio-graphene-nanocomposite
#18
Xiaohui Zhang, Zheng Zhang, Qingliang Liao, Shuo Liu, Zhuo Kang, Yue Zhang
Ni/NiO nanoflower modified reduced graphene oxide (rGO) nanocomposite (Ni/NiO-rGO) was introduced to screen printed electrode (SPE) for the construction of a nonenzymatic electrochemical glucose biosensor. The Ni/NiO-rGO nanocomposite was synthesized by an in situ reduction process. Graphene oxide (GO) hybrid Nafion sheets first chemical adsorbed Ni ions and assembled on the SPE. Subsequently, GO and Ni ions were reduced by hydrazine hydrate. The electrochemical properties of such a Ni/NiO-rGO modified SPE were carefully investigated...
October 26, 2016: Sensors
https://www.readbyqxmd.com/read/27782067/one-step-assembly-of-thin-films-of-carbon-nanotubes-on-screen-printed-interface-for-electrochemical-aptasensing-of-breast-cancer-biomarker
#19
Muhammad Azhar Hayat Nawaz, Sajid Rauf, Gaelle Catanante, Mian Hasnain Nawaz, Gilvanda Nunes, Jean Louis Marty, Akhtar Hayat
Thin films of organic moiety functionalized carbon nanotubes (CNTs) from a very well-dispersed aqueous solution were designed on a screen printed transducer surface through a single step directed assembly methodology. Very high density of CNTs was obtained on the screen printed electrode surface, with the formation of a thin and uniform layer on transducer substrate. Functionalized CNTs were characterized by X-ray diffraction spectroscopy (XRD), Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA) and Brunauer-Emmett- Teller (BET) surface area analyzer methodologies, while CNT coated screen printed transducer platform was analyzed by scanning electron microscopy (SEM), atomic force microscopy (AFM), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS)...
October 6, 2016: Sensors
https://www.readbyqxmd.com/read/27775661/printable-electrochemical-biosensors-a-focus-on-screen-printed-electrodes-and-their-application
#20
Keiichiro Yamanaka, Mun'delanji C Vestergaard, Eiichi Tamiya
In this review we present electrochemical biosensor developments, focusing on screen-printed electrodes (SPEs) and their applications. In particular, we discuss how SPEs enable simple integration, and the portability needed for on-field applications. First, we briefly discuss the general concept of biosensors and quickly move on to electrochemical biosensors. Drawing from research undertaken in this area, we cover the development of electrochemical DNA biosensors in great detail. Through specific examples, we describe the fabrication and surface modification of printed electrodes for sensitive and selective detection of targeted DNA sequences, as well as integration with reverse transcription-polymerase chain reaction (RT-PCR)...
October 21, 2016: Sensors
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