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https://www.readbyqxmd.com/read/28324956/a-graphene-oxide-ph-sensor-for-wound-monitoring
#1
B Melai, P Salvo, N Calisi, L Moni, A Bonini, C Paoletti, T Lomonaco, V Mollica, R Fuoco, F Di Francesco
This article describes the fabrication and characterization of a pH sensor for monitoring the wound status. The pH sensitive layer consists of a graphene oxide (GO) layer obtained by drop-casting 5 μΐ of GO dispersion onto the working electrode of a screen-printed substrate. Sensitivity was 31.8 mV/pH with an accuracy of 0.3 unit of pH. Open-circuit potentiometry was carried out to measure pH in an exudate sample. The GO pH sensor proved to be reliable as the comparison with results obtained from a standard glass electrode pH-meter showed negligible differences (<; 0...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28319900/bipolar-electrochemiluminescence-on-thread-a-new-class-of-electroanalytical-sensors
#2
Rui Liu, Cuiling Liu, Huijie Li, Min Liu, Dan Wang, Chunsun Zhang
This paper introduces a new and simple concept for fabricating low-cost, easy-to-use capillary microchannel (CMC) assisted thread-based microfluidic analytical devices (CMCA-μTADs) for bipolar electrochemiluminescence (BP-ECL) application. The thread with patterns of carbon screen-printed electrodes and bare thread zones (BTZs) is embedded into a CMC. Such CMCA-μTADs can produce a strong and stable BP-ECL signal, and have an extremely low cost ($0.01 per device). Interestingly, the CMCA-μTADs are ultraflexible, and can be bent with a 135° bending angle at the BTZ or with a 150° bending angle at the middle of bipolar electrode (BPE), with no loss of analytical performance...
March 7, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28302974/effect-of-the-chain-length-of-a-modified-layer-and-surface-roughness-of-an-electrode-on-impedimetric-immunosensors
#3
Chia-Hung Lin, Ming-Jie Lin, Ching-Chou Wu
An ultrasensitive label-free impedimetric immunosensor is constructed by modifying a 3-mercaptoproponic acid (MPA) monolayer on highly rough gold nanostructure (AuNS)-electrodeposited screen printed carbon electrodes (SPCEs) for the detection of small molecular weight drugs (SMWDs), such as salbutamol (SAL). The SPCEs preoxidized in a 0.1 M H2SO4 solution (called po-SPCEH2SO4) are electrodeposited with the AuNS to increase the roughness factor to 23.64 ± 1.76, larger than the AuNS/po-SPCENaOH or the AuNS/po-SPCEPBS...
2017: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
https://www.readbyqxmd.com/read/28300229/impedance-spectroscopy-for-monosaccharides-detection-using-responsive-hydrogel-modified-paper-based-electrodes
#4
C M Daikuzono, C Delaney, H Tesfay, L Florea, O N Oliveira, A Morrin, D Diamond
Herein we present a novel sensor for the detection of monosaccharides (e.g. glucose, fructose) in solution, using electrical impedance spectroscopy. The sensor is based on carbon interdigitated electrodes, printed on paper using screen printing. The surface of the electrodes was modified with a thin layer of hydrogel containing acrylamide copolymerised with 20 mol% 3-(Acrylamido)phenylboronic acid (PBA). It was observed that the hydrogel layers containing 20 mol% PBA swell considerably in the presence of glucose and fructose...
March 16, 2017: Analyst
https://www.readbyqxmd.com/read/28299685/fabrication-of-lab-on-paper-using-porous-au-paper-electrode-application-to-tumor-marker-electrochemical-immunoassays
#5
Shenguang Ge, Yan Zhang, Mei Yan, Jiadong Huang, Jinghua Yu
A simple, low-cost, and sensitive electrochemical lab-on-paper assay is developed based on a novel gold nanoparticle modified porous paper working electrode for use in point-of-care testing (POCT). Electrochemical methods are introduced for lab-on-paper based on screen-printed paper electrodes. To further improve specificity, performance, and sensitivity for point-of-care testing, a novel porous Au-paper working electrode (Au-PWE) is designed for lab-on-paper using growth of an interconnected Au nanoparticle (NP) layer on the surface of cellulose fibers in order to enhance the conductivity of the paper sample zone and immobilize the primary antibodies (Ab1)...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28299681/screen-printed-all-polymer-aptasensor-for-impedance-based-detection-of-influenza-a-virus
#6
Julie Kirkegaard, Noemi Rozlosnik
In this chapter a detailed description of the fabrication and testing of an aptasensor for influenza A virus detection is given. The sensor chip is an all-polymer chip fabricated with screen-printed poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) electrodes. Chip substrates are made by CO2 laser cutting of Poly(methyl methacrylate) (PMMA) sheets. Influenza A virus specific aptamers are immobilized onto the electrodes by UV cross-linking. Impedance based measurements at a single frequency, measured over time, are used to detect the virus in a buffer solution...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28299677/a-reagentless-screen-printed-amperometric-biosensor-for-the-determination-of-glutamate-in-food-and-clinical-applications
#7
G Hughes, R M Pemberton, P R Fielden, J P Hart
A reagentless biosensor has been successfully developed to measure glutamate in food and clinical samples. The enzyme, glutamate dehydrogenase (GLDH) and the cofactor, nicotinamide adenine dinucleotide (NAD(+)) are fully integrated onto the surface of a Meldola's Blue screen-printed carbon electrode (MB-SPCE). The biological components are immobilized by utilizing unpurified multi-walled carbon nanotubes (MWCNT's) mixed with the biopolymer chitosan (CHIT), which are drop-coated onto the surface of the MB-SPCE in a layer-by-layer fashion...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28293725/a-nano-au-c-mwcnt-based-label-free-amperometric-immunosensor-for-the-detection-of-capsicum-chlorosis-virus-in-bell-pepper
#8
Anshul Sharma, Ankur Kaushal, Saurabh Kulshrestha
Accurate and on time diagnosis of plant viruses is an essential prerequisite for efficient control in field conditions. A number of diagnostic methods have been reported with the required level of sensitivity. Here, we propose a label free immunosensor for efficient and sensitive detection of capsicum chlorosis virus (CaCV) in bell pepper. Antigen was immobilized over the surface of gold nanoparticle/multi-walled carbon nanotube (Nano-Au/C-MWCNT) screen printed electrodes using 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC)/N-hydroxysuccinimide (NHS) cross linking chemistry followed by interaction with groundnut bud necrosis virus (GBNV)/CaCV specific polyclonal antibody...
March 14, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28273937/an-electrochemical-method-for-sensitive-and-rapid-detection-of-fam134b-protein-in-colon-cancer-samples
#9
Farhadul Islam, Md Hakimul Haque, Sharda Yadav, Md Nazmul Islam, Vinod Gopalan, Nam-Trung Nguyen, Alfred K Lam, Muhammad J A Shiddiky
Despite the excellent diagnostic applications of the current conventional immunoassay methods such as ELISA, immunostaining and Western blot for FAM134B detection, they are laborious, expensive and required a long turnaround time. Here, we report an electrochemical approach for rapid, sensitive, and specific detection of FAM134B protein in biological (colon cancer cell extracts) and clinical (serum) samples. The approach utilises a differential pulse voltammetry (DPV) in the presence of the [Fe(CN)6](3-/4-) redox system to quantify the FAM134B protein in a two-step strategy that involves (i) initial attachment of FAM134B antibody on the surface of extravidin-modified screen-printed carbon electrode, and (ii) subsequent detection of FAM134B protein present in the biological/clinical samples...
December 2017: Scientific Reports
https://www.readbyqxmd.com/read/28268713/investigations-on-different-epoxies-for-electrical-insulation-of-microflex-structures
#10
P Cvancara, S Lauser, L Rudmann, T Stieglitz
The microflex interconnection (MFI) technique is often used to connect electrically and mechanically thin film ribbons or electrodes with a solid substrate like screen printed ceramics. For stabilization reasons epoxy is used to fix the MFI structure. As epoxy tends to form cracks when surrounded by water or electrolytes we are eager to find an epoxy which provides sufficient insulation between the single channels of the MFI structure also in a moist surrounding. Therefore we designed a device to investigate the insulating properties of different epoxies (Uhu Plus Endfest 300, Epo-Tek 353ND and 353ND-T) immersed in saline solution...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28268338/origami-microfluidic-paper-analytical-devices-ompad-for-sensing-and-diagnostics
#11
Meera Punjiya, Chung Hee Moon, Yu Chen, Sameer Sonkusale
Recent research activities in the area of low-cost sensing and diagnostics that are realized on cellulosic paper substrate are presented. First a three-dimensional origami paper-based analytical device (omPAD) with multiple electrochemical sensors, an integrated sample reservoir and tight integration with a custom CMOS potentiostat is presented. Second, an optical sensor array with built-in microfluidic channel for sample delivery is presented. The sensors are fabricated using a combination of wax printing and screen-printing using a solution based approach in ambient conditions without the need for expensive fabrication equipment or a cleanroom...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28262862/determination-of-mercury-ii-in-water-at-sub-nanomolar-levels-by-laser-ablation-icpms-analysis-of-screen-printed-electrodes-used-as-a-portable-voltammetric-preconcentration-system
#12
Zuriñe Abrego, Nora Unceta, Alicia Sánchez, Alberto Gómez-Caballero, Luis Maria Berrio-Ochoa, M Aranzazu Goicolea, Ramón J Barrio
Environmental pollution by mercury in ambient water samples is a recognized problem worldwide. Sample preservation and transport to the laboratory lead to uncertain analytical results. This study outlines the development of a procedure for on-site electrodeposition of mercury from water samples on a screen-printed gold electrode (SPGE) using portable voltammetric techniques. Once in the laboratory, Hg is analyzed by laser ablation inductively coupled plasma-mass spectrometry (LA-ICPMS) in order to ensure that the required sensitivity and precision levels for environmental sample analysis are reached...
March 6, 2017: Analyst
https://www.readbyqxmd.com/read/28256671/ratiometric-electrochemical-detection-of-hydrogen-peroxide-and-glucose
#13
Sean Goggins, Ellen A Apsey, Mary F Mahon, Christopher G Frost
Hydrogen peroxide (H2O2) detection is of high importance as it is a versatile (bio)marker whose detection can indicate the presence of explosives, enzyme activity and cell signalling pathways. Herein, we demonstrate the rapid and accurate ratiometric electrochemical detection of H2O2 using disposable screen-printed electrodes through a reaction-based indicator assay. Ferrocene derivatives equipped with self-immolative linkers and boronic acid ester moieties were synthesised and tested, and, through a thorough assay optimisation, the optimum probe showed good stability, sensitivity and selectivity towards H2O2...
March 3, 2017: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/28254662/sensors-based-on-mesoporous-sno2-cuwo4-with-high-selective-sensitivity-to-h2s-at-low-operating-temperature
#14
Adelina Stanoiu, Cristian E Simion, Jose Maria Calderon-Moreno, Petre Osiceanu, Mihaela Florea, Valentin S Teodorescu, Simona Somacescu
Development of new sensitive materials by different synthesis routes in order to emphasize the sensing properties for hazardous H2S detection is one of a nowadays challenge in the field of gas sensors. In this study we obtained mesoporous SnO2-CuWO4 with selective sensitivity to H2S by an inexpensive synthesis route with low environmental pollution level, using tripropylamine (TPA) as template and polyvinylpyrrolidone (PVP) as dispersant/stabilizer. In order to bring insights about the intrinsic properties, the powders were characterized by means of a variety of complementary techniques such as: X-Ray Diffraction, XRD; Transmission Electron Microscopy, TEM; High Resolution TEM, HRTEM; Raman Spectroscopy, RS; Porosity Analysis by N2 adsorption/desorption, BET; Scanning Electron Microscopy, SEM and X-ray Photoelectron Spectroscopy, XPS...
February 24, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28254335/magnetism-assisted-modification-of-screen-printed-electrode-with-magnetic-multi-walled-carbon-nanotubes-for-electrochemical-determination-of-dopamine
#15
Yong-Mei Zhang, Pei-Li Xu, Qiong Zeng, Yi-Ming Liu, Xun Liao, Mei-Fang Hou
A simple and sensitive dopamine (DA) electrochemical sensor was fabricated based on magnetism-assisted modification of screen printed electrode (SPE) with magnetic multi-walled carbon nanotubes (mMWCNTs). The mMWCNTs modified electrodes (mMWCNTs/SPE) combines the advantages of SPE and the simultaneous contribution of magnetic nanoparticles (MNPs) and MWCNTs, increasing sensitivity and selectivity of DA detection. The linearity was found between 5μM to 180μM, with the limit of detection (LOD) of 0.43μM. In the mean time, this modified electrode exhibited excellent selectivity for DA detection with almost no interference from ascorbic acid (AA), which co-exists with DA in many bio-samples and causes common interference...
May 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28254318/detection-of-esat-6-by-a-label-free-miniature-immuno-electrochemical-biosensor-as-a-diagnostic-tool-for-tuberculosis
#16
Mohamed Fethi Diouani, Oussama Ouerghi, Amira Refai, Kamel Belgacem, Chaker Tlili, Dhafer Laouini, Makram Essafi
Tuberculosis is a worldwide disease considered as a major health problem with high morbidity and mortality rates. Poor detection of Mycobacterium tuberculosis (Mtb), the causative agent of tuberculosis remains a major obstacle to the global control of this disease. Here we report the development of a new test based on the detection of the major virulent factor of Mtb, namely the early secreted antigenic target 6-kDa protein or ESAT-6. A label free electrochemical immunosensor using an anti-ESAT-6 monoclonal antibody as a bio-receptor is described herein...
May 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28248479/functionally-graded-bismuth-oxide-zirconia-bilayer-electrolytes-for-high-performance-intermediate-temperature-solid-oxide-fuel-cells-it-sofcs
#17
Dong Woo Joh, Jeong Hwa Park, Doyeub Kim, Eric D Wachsman, Kang Taek Lee
A functionally graded Bi1.6Er0.4O3 (ESB)/Y0.16Zr0.84O1.92 (YSZ) bilayer electrolyte is successfully developed via a cost-effective screen printing process using nanoscale ESB powders on the tape-cast NiO-YSZ anode support. Because of the highly enhanced oxygen incorporation process at the cathode/electrolyte interface, a novel bilayer solid oxide fuel cell (SOFC) yields extremely high power density of ∼2.1 W cm(-2) at 700 °C, which is a 2.4 times increase compared to that of the YSZ single electrolyte SOFC...
March 6, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28237900/synthesis-of-novel-monomeric-graphene-quantum-dots-and-corresponding-nanocomposite-with-molecularly-imprinted-polymer-for-electrochemical-detection-of-an-anticancerous-ifosfamide-drug
#18
Bhim Bali Prasad, Anil Kumar, Ragini Singh
This paper reports a typical synthesis of a nanocomposite of functionalized graphene quantum dots and imprinted polymer at the surface of screen-printed carbon electrode using N-acryloyl-4-aminobenzamide, as a functional monomer, and an anticancerous drug, ifosfamide, as a print molecule (test analyte). Herein, graphene quantum dots in nanocomposite practically induced the electrocatalytic activity by lowering the oxidation overpotential of test analyte and thereby amplifying electronic transmission, without any interfacial barrier in between the film and the electrode surface...
February 20, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28228002/custom-shaped-organic-photovoltaic-modules-freedom-of-design-by-printing
#19
M Välimäki, E Jansson, P Korhonen, A Peltoniemi, S Rousu
Freedom of design that was introduced as organic photovoltaic (OPV) modules were fabricated by printing. As proof-of-concept, we show OPV leaf fabrication in A5 size using gravure and rotary screen printing processes for the main active layers of the OPV structure. These printing methods allow direct printing of any kind of arbitrary, two-dimensional shapes including patterning of the electric contacts thus post-patterning stages are not needed. Fabrication of custom-shaped OPV modules requires detailed information about the technical boundaries set by the manufacturing process and materials which in turn influence the layout design and R2R upscaling...
December 2017: Nanoscale Research Letters
https://www.readbyqxmd.com/read/28226901/investigations-on-different-epoxies-for-electrical-insulation-of-microflex-structures
#20
P Cvancara, S Lauser, L Rudmann, T Stieglitz, P Cvancara, S Lauser, L Rudmann, T Stieglitz, P Cvancara, S Lauser, T Stieglitz, L Rudmann
The microflex interconnection (MFI) technique is often used to connect electrically and mechanically thin film ribbons or electrodes with a solid substrate like screen printed ceramics. For stabilization reasons epoxy is used to fix the MFI structure. As epoxy tends to form cracks when surrounded by water or electrolytes we are eager to find an epoxy which provides sufficient insulation between the single channels of the MFI structure also in a moist surrounding. Therefore we designed a device to investigate the insulating properties of different epoxies (Uhu Plus Endfest 300, Epo-Tek 353ND and 353ND-T) immersed in saline solution...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
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