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María L Scala-Benuzzi, Julio Raba, Galo J A A Soler-Illia, Rudolf J Schneider, Germán A Messina
We report a novel and innovative electrochemical paper-based immunocapture assay (EPIA) to address the need for ultrasensitive detection of emerging pollutants without regulatory status and whose effects on environment and human health are not completely yet understood. In particular, we present the application of this system towards highly sensitive detection of the emerging pollutant ethinyl estradiol (EE2). The EPIA approach is based on the use of paper microzones modified with silica nanoparticles (SNs) and anti-EE2 specific antibodies for capture and pre-concentration of EE2 from river water samples...
February 23, 2018: Analytical Chemistry
Seongkyu Song, Hyunseok Shim, Sang Kyoo Lim, Soon Moon Jeong
We demonstrate wide colour tunability of polydimethylsiloxane-based alternating-current-driven electroluminescent devices with intrinsically stretchable characteristics achieved by simply modulating the electrical frequency. By employing both a screen-printed emitting layer and frequency-dependent colour tuning of ZnS:Cu-based phosphors, we demonstrate various coloured patterned images in a single device. We also show enhanced colour-tuning performance by mixing multi-colour phosphors, which results in a broad range of available coordinates in colour space...
February 20, 2018: Scientific Reports
Maher Sayhi, Oussama Ouerghi, Kamel Belgacem, Marwa Arbi, Yudum Tepeli, Abdeljalil Ghram, Ülkü Anik, Lars Österlund, Dhafer Laouini, Mohamed Fethi Diouani
Influenza is a viral infectious disease considered as a source of many health problems and enormous socioeconomic disruptions. Conventional methods are inadequate for in-field detection of the virus and generally suffer from being laborious and time-consuming. Thus, studies aiming to develop effective alternatives to conventional methods are urgently needed. In this work, we developed an approach for the isolation and detection of influenza A virus subtype H9N2. For this aim, two specific influenza receptors were used...
February 7, 2018: Biosensors & Bioelectronics
Guiyin Li, Shanshan Li, Zhihong Wang, Yewei Xue, Chenyang Dong, Junxiang Zeng, Yong Huang, Jintao Liang, Zhide Zhou
Sensitive and accurate detection of tumor markers is critical to early diagnosis, point-of-care and portable medical supervision. Alpha fetoprotein (AFP) is an important clinical tumor marker for hepatocellular carcinoma (HCC), and the concentration of AFP in human serum is related to the stage of HCC. In this paper, a label-free electrochemical aptasensor for AFP detection was fabricated using AFP-aptamer as the recognition molecule and thionin/reduced graphene oxide/gold nanoparticles (TH/RGO/Au NPs) as the sensor platform...
February 13, 2018: Analytical Biochemistry
Shikha Sharma, Nidhi Singh, Vartika Tomar, Ramesh Chandra
Serotonin is one of the important neurotransmitters of our body. It's abnormal concentration is associated with multiple disorders and diseases. Sensitive and precise electrochemical determination of serotonin is not possible with bare working electrodes due to various reasons viz. electro-chemical fouling, presence of other biological molecules having similar oxidation potential, and lower concentration of serotonin in biological samples. Surface modification of working electrode is required for fast, precise, selective, and sensitive detection of serotonin...
February 6, 2018: Biosensors & Bioelectronics
Mohadeseh Rahimi-Mohseni, Jahan Bakhsh Raoof, Reza Ojani, Tahereh A Aghajanzadeh, Ayemeh Bagheri Hashkavayi
A new and facile method for electrochemical determination of L-tyrosine was designed. Tyrosinase from banana peel tissue was immobilized at fiber matrices of the paper disc via physical adsorption. The mediator potassium hexacyanoferrate and 3-mercaptopropyl trimethoxysilane-functionalized silica nanoparticles were added to the paper disc, respectively. The modified paper disc was placed on the top of the graphite screen printed electrode and electrochemical characterization of this biosensor was studied by cyclic voltammetry and electrochemical impedance spectroscopy methods...
February 12, 2018: International Journal of Biological Macromolecules
Li Ren'ai, Kaili Zhang, Guangxue Chen, Bin Su, Junfei Tian, Minghui He, Fachuang Lu
We report a green fabrication of conductive paper based on in situ polymerization of polymerizable deep eutectic solvents (PDESs) through a screen printing process. By pre-designed circuit paths and careful integration, on-demand input/output 3D circuits can be achieved, showing its flexibility to origami electronics.
February 15, 2018: Chemical Communications: Chem Comm
Samar Damiati, Martin Peacock, Stefan Leonhardt, Laila Damiati, Mohammed A Baghdadi, Holger Becker, Rimantas Kodzius, Bernhard Schuster
Hepatic oval cells (HOCs) are considered the progeny of the intrahepatic stem cells that are found in a small population in the liver after hepatocyte proliferation is inhibited. Due to their small number, isolation and capture of these cells constitute a challenging task for immunosensor technology. This work describes the development of a 3D-printed continuous flow system and exploits disposable screen-printed electrodes for the rapid detection of HOCs that over-express the OV6 marker on their membrane. Multiwall carbon nanotube (MWCNT) electrodes have a chitosan film that serves as a scaffold for the immobilization of oval cell marker antibodies (anti-OV6-Ab), which enhance the sensitivity of the biomarker and makes the designed sensor specific for oval cells...
February 14, 2018: Genes
Chen-Su Chiang, Yia-Ming Wu, Wen-Hsi Lee
Solar cell is one of the most popular alternative energies. The aim of this study is to construct an ohmic contact between front electrode and Si-based solar cell by a Newly-invented low-cost paste and low temperature sintering process. The core-shell of CucoreAgshell powders were prepared for making high solid content paste, then screen printing the fine line on laser-opening H-pattern silicon substrate and applying firing process. Because the silver coverage is more than 95% and silver nanoparticles start to melt at 200 °C...
April 1, 2018: Journal of Nanoscience and Nanotechnology
Keerthy Dhara, T Ramachandran, Bipin G Nair, T G Satheesh Babu
A highly sensitive nonenzymatic hydrogen peroxide (H2O2) sensor was fabricated using platinum nanoparticles decorated reduced graphene oxide (Pt/rGO) nanocomposite. The Pt/rGO nanocomposite was prepared by single-step chemical reduction method. Nanocomposite was characterized by various analytical techniques including Raman spectroscopy, X-ray diffraction, field emission scanning electron microscope and high-resolution transmission electron microscopy. Screen printed electrodes (SPEs) were fabricated and the nanocomposite was cast on the working area of the SPE...
June 1, 2018: Journal of Nanoscience and Nanotechnology
Inge Verboven, Jeroen Stryckers, Viktorija Mecnika, Glen Vandevenne, Manoj Jose, Wim Deferme
To maintain typical textile properties, smart designs of light emitting devices are printed directly onto textile substrates. A first approach shows improved designs for alternating current powder electroluminescence (ACPEL) devices. A configuration with the following build-up, starting from the textile substrate, was applied using the screen printing technique: silver (10 µm)/barium titanate (10 µm)/zinc-oxide (10 µm) and poly(3,4-ethylenedioxythiophene)poly(styrenesulfonate) (10 µm). Textile properties such as flexibility, drapability and air permeability are preserved by implementing a pixel-like design of the printed layers...
February 13, 2018: Materials
V Poza-Nogueiras, M Arellano, E Rosales, M Pazos, E González-Romero, M A Sanromán
Conventional water treatments are generally inadequate for degradation of emerging pollutants such as ionic liquids (ILs). The use of heterogeneous electro-Fenton (HEF) has attracted great interest, due to its ability to efficiently oxidize a wide range of organic pollutants operating in cycles or in continuous mode. In this study, the removal of a complex IL from the imidazolinium family (1,3-Bis(2,4,6-trimethylphenyl)imidazolinium chloride), by means of HEF using iron alginate spheres as catalyst has been investigated, resulting in significant TOC decay after 6 h...
February 8, 2018: Chemosphere
Yang Wang, Jinping Luo, Juntao Liu, Xinrong Li, Zhuang Kong, Hongyan Jin, Xinxia Cai
17β-estradiol (17β-E2) plays a critical role in regulating reproduction in human, there is therefore an urgent need to detect it sensitively and precisely in a cost-effective and easy method. In this paper, a label-free integrated microfluidic paper-based analytical device was developed for highly sensitive electrochemical detection of 17β-E2. The microfluidic channel of the paper-based sensor was fabricated with wax printing and the three electrodes, including working, counter and reference electrode were screen-printed...
February 3, 2018: Biosensors & Bioelectronics
Mohammad Johari-Ahar, Pari Karami, Mostafa Ghanei, Abbas Afkhami, Hasan Bagheri
This work demonstrates the development of a gold screen-printed electrode (Au-SPE)-based biosensor modified with a molecularly imprinted polymer and amplified using antibody-conjugated nano-liposomes. The developed biosensor was utilized for dual determination of epidermal growth factor receptor (EGFR) and vascular endothelial growth factor (VEGF) as cancer biomarkers. To prepare this biosensor, Au-SPE was modified with 3,3'-dithiodipropionic acid di(N-hydroxysuccinimide ester) via self-assembly method and then the target proteins (EGFR and VEGF) were covalently attached to the modified SPE...
February 3, 2018: Biosensors & Bioelectronics
Elena Fernández, Lorena Vidal, Antonio Canals
A novel approach is presented to determine hydrophilic phenols in olive oil samples, employing vortex-assisted reversed-phase dispersive liquid-liquid microextraction (RP-DLLME) for sample preparation and screen-printed carbon electrodes for voltammetric analysis. The oxidation of oleuropein, hydroxytyrosol, caffeic acid, ferulic acid and tyrosol was investigated, being caffeic acid and tyrosol selected for quantification. A matrix-matching calibration using sunflower oil as analyte-free sample diluted with hexane was employed to compensate matrix effects...
May 1, 2018: Talanta
Xin Li, Xinlei Wang, Tian Fang, Lizhi Zhang, Jingming Gong
Owing to the lack of chromophores and ionizable functional groups, it is a significant challenge to determine perfluorooctane sulfonyl fluoride (PFOSF) by traditional high-performance liquid chromatography or liquid chromatography/mass spectrometry, especially at a low concentration. In this work a unique photoelectrochemical (PEC) sensing strip has been developed for the first time for the detection of PFOSF. The sensing strip is cost effective and disposable, whereby BiOI nanoflake arrays are fabricated on a home-made screen-printed electrode through a facile one-step in-situ electrodeposition process, and then the molecule tags (i...
May 1, 2018: Talanta
K L Westmacott, A Crew, O Doran, J P Hart
This paper describes the development of a novel electrochemical assay for the measurement of water-soluble vitamins in food and pharmaceutical products. The optimum conditions for the determination of vitamin B 1 (thiamine), B 2 (riboflavin) and B 6 (pyridoxine) in phosphate buffer were established using cyclic voltammetry in conjunction with screen printed carbon electrodes (SPCEs). The optimum current response for all three vitamins was achieved in 0.1M phosphate buffer pH 11 using an initial potential of -1...
May 1, 2018: Talanta
Yaoguang Wang, Guanhui Zhao, Huan Wang, Wei Cao, Bin Du, Qin Wei
In this study, a sensitive sandwich-type electrochemical immunosensor was fabricated for the detection of alpha fetoprotein (AFP) based on Co3O4@MnO2-thionine (Co3O4@MnO2-Th) and the screen printing technique. Meanwhile, the pseudo enzyme-linked immunosorbent assay (pseudo-ELISA) method was applied in fabricating the immunosensor. Screen-printed carbon electrode (SPCE) was applied for achieving the detection of AFP. Simultaneously, the amino functionalized Co3O4@MnO2-Th was employed as secondary label and could greatly improve the electrochemical response signal, which was beneficial for detecting AFP...
February 3, 2018: Biosensors & Bioelectronics
Shikha Sharma, Julia Zapatero-Rodríguez, Rahul Saxena, Richard O'Kennedy, Sudha Srivastava
Assesment of human epidermal growth factor receptor 2 status is a key factor prompting definitive treatment decisions that help in reducing mortality rates associated with breast cancer. In this article, highly sensitive and low-cost impedimetric immunosensor using single-chain fragment variable antibody fragments was developed for quantitative detection of human epidermal growth factor receptor 2 from serum employing gold nanoparticle-modified disposable screen-printed carbon electrodes. The gold nanoparticles facilitate fast electron transfer and offer a biocompatible surface for immobilization of small antibody fragments in an oriented manner, resulting in improved antigen binding efficiency...
January 31, 2018: Biosensors & Bioelectronics
Dignya Desai, Ashok Kumar, Debajyoti Bose, Manali Datta
A facile label free, ultrasensitive platform for a rapid detection of chronic kidney disease has been fabricated. Early intervention in patients with chronic kidney disease has the potential to delay, or even prevent, the development of end stage renal disease and complications, leading to a marked impact on life expectancy and quality of life. Thus, a potable electrochemical diagnostic biosensor has become an attractive option as electrochemical analysis is feasible to use for on-site detection of samples...
May 15, 2018: Biosensors & Bioelectronics
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