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Seong Je Cho, Hui-Bog Noh, Mi-Sook Won, Chul-Ho Cho, Kwang Bok Kim, Yoon-Bo Shim
A selective nonenzymatic glucose sensor was developed based on the direct oxidation of glucose on hierarchical CuCo bimetal-coated with a glucose-imprinted polymer (GIP). Glucose was introduced into the GIP composed of Nafion and polyurethane along with aminophenyl boronic acid (APBA), which was formed on the bimetal electrode formed on a screen-printed electrode. The extraction of glucose from the GIP allowed for the selective permeation of glucose into the bimetal electrode surface for oxidation. The GIP-coated bimetal sensor probe was characterized using electrochemical and surface analytical methods...
August 10, 2017: Biosensors & Bioelectronics
Daniel Martín-Yerga, Alejandro Pérez-Junquera, David Hernández-Santos, Pablo Fanjul-Bolado
Real-time spectroelectrochemistry of [Ru(bpy)3](2+) electroluminescence showed a strong correlation with electrochemical processes occurring at metal screen-printed electrodes. Luminescence was quenched when the metal oxidation takes place, but it behaved differently when gold or silver were reduced, which suggests that changes in the structural characteristics of metallic electrodes play a decisive role in luminescence spectroelectrochemistry.
August 18, 2017: Physical Chemistry Chemical Physics: PCCP
Zheng-Hu Shi, Xiao-Qing Zhang, Qian-Na Zhen, Ming Zuo, Gang Tian, Yi-Fan He, Min Ding
Metanephrines (MNs) were suggested as a potential first-line biochemical index for the diagnosis of phaeochromocytomas (PHEO). In this study, we developed a simple electrochemical method for the quantitative measurement of MNs in spot urine samples. As MNs contain a hydroxyphenyl group, they could be oxidized at a certain potential to quinines, which could be further detected by the differential pulse voltammetry (DPV) method using unmodified screen-printed carbon electrode (SPCE). Meanwhile, the solid phase extraction (SPE) technique was used to eliminate the matrix effect in the samples...
August 14, 2017: Scientific Reports
F R Caetano, E A Carneiro, D Agustini, L C S Figueiredo-Filho, C E Banks, M F Bergamini, L H Marcolino-Junior
Microfluidic devices constructed using low cost materials presents as alternative for conventional flow analysis systems because they provide advantages as low consumption of reagents and samples, high speed of analysis, possibility of portability and the easiness of construction and maintenance. Herein, is described for the first time the use of an electrochemical biosensor for phenol detection combined with a very simple and efficient microfluidic device based on commercial textile threads. Taking advantages of capillary phenomena and gravity forces, the solution transportation is promoted without any external forces or injection pump...
July 29, 2017: Biosensors & Bioelectronics
Georges Dubourg, Apostolos Segkos, Jaroslav Katona, Marko Radović, Slavica Savić, Georgios Niarchos, Christos Tsamis, Vesna Crnojević-Bengin
This paper describes the fabrication and the characterization of an original example of a miniaturized resistive-type humidity sensor, printed on flexible substrate in a large-scale manner. The fabrication process involves laser ablation for the design of interdigitated electrodes on PET (Poly-Ethylene Terephthalate) substrate and a screen-printing process for the deposition of the sensitive material, which is based on TiO₂ nanoparticles. The laser ablation process was carefully optimized to obtain micro-scale and well-resolved electrodes on PET substrate...
August 11, 2017: Sensors
Mahbubur Rahman, Lee Yook Heng, Dedi Futra, Chew Poh Chiang, Zulkafli A Rashid, Tan Ling Ling
The present research describes a simple method for the identification of the gender of arowana fish (Scleropages formosus). The DNA biosensor was able to detect specific DNA sequence at extremely low level down to atto M regimes. An electrochemical DNA biosensor based on acrylic microsphere-gold nanoparticle (AcMP-AuNP) hybrid composite was fabricated. Hydrophobic poly(n-butylacrylate-N-acryloxysuccinimide) microspheres were synthesised with a facile and well-established one-step photopolymerization procedure and physically adsorbed on the AuNPs at the surface of a carbon screen printed electrode (SPE)...
August 10, 2017: Nanoscale Research Letters
Zuzana Koudelkova, Tomas Syrovy, Pavlina Ambrozova, Zdenek Moravec, Lubomir Kubac, David Hynek, Lukas Richtera, Vojtech Adam
In this study, the preparation and electrochemical application of a chromium(III) oxide modified carbon paste electrode (Cr-CPE) and a screen printed electrode (SPE), made from the same material and optimized for the simple, cheap and sensitive simultaneous determination of zinc, cadmium, lead, copper and the detection of silver ions, is described. The limits of detection and quantification were 25 and 80 µg·L(-1) for Zn(II), 3 and 10 µg·L(-1) for Cd(II), 3 and 10 µg·L(-1) for Pb(II), 3 and 10 µg·L(-1) for Cu(II), and 3 and 10 µg·L(-1) for Ag(I), respectively...
August 9, 2017: Sensors
Thitirat Putnin, Watthanachai Jumpathong, Rawiwan Laocharoensuk, Jaroon Jakmunee, Kontad Ounnunkad
This work focuses on fabricating poly(2-aminobenzylamine)-modified screen-printed carbon electrode as an electrochemical immunosensor for the label-free detection of human immunoglobulin G. To selectively detect immunoglobulin G, the anti-immunoglobulin G antibody with high affinity to immunoglobulin G was covalently linked with the amine group of poly(2-aminobenzylamine) film-deposited screen-printed carbon electrode. The selectivity for immunoglobulin G was subsequently assured by being challenged with redox-active interferences and adventitious adsorption did not significantly interfere the analyte signal...
August 6, 2017: Artificial Cells, Nanomedicine, and Biotechnology
Kasra Saeedfar, Lee Yook Heng, Chew Poh Chiang
Multi-wall carbon nanotubes (MWCNTs) were modified to design a new DNA biosensor. Functionalized MWCNTs were equipped with gold nanoparticles (GNPs) (~15nm) (GNP-MWCNTCOOH) to construct DNA biosensors based on carbon-paste screen-printed (SPE) electrodes. GNP attachment onto functionalized MWCNTs was carried out by microwave irradiation and was confirmed by spectroscopic studies and surface analysis. DNA biosensors based on differential pulse voltammetry (DPV) were constructed by immobilizing thiolated single-stranded DNA probes onto GNP-MWCNTCOOH...
July 31, 2017: Bioelectrochemistry
Sorachon Yoriya, Angkana Chumphu, Pusit Pookmanee, Wreerat Laithong, Sirichai Thepa, Roongrojana Songprakorp
Crystalline TiO₂ has shown its great photocatalytic properties in bacterial inactivation. This work presents a design fabrication of low-cost, layered TiO₂ films assembled reactors and a study of their performance for a better understanding to elucidate the photocatalytic effect on inactivation of E. coli in water. The ability to reduce the number of bacteria in water samples for the layered TiO₂ composing reactors has been investigated as a function of time, while varying the parameters of light sources, initial concentration of bacteria, and ratios of TiO₂ film area and volume of water...
September 30, 2016: Materials
Hao Wu, Biao Tang, Robert A Hayes, Yingying Dou, Yuanyuan Guo, Hongwei Jiang, Guofu Zhou
Industrialization of electrofluidic devices requires both high performance coating laminates and efficient material utilization on large area substrates. Here we show that screen printing can be effectively used to provide homogeneous pin-hole free patterned amorphous fluoropolymer dielectric layers to provide both the insulating and fluidic reversibility required for devices. Subsequently, we over-coat photoresist using slit coating on this normally extremely hydrophobic layer. In this way, we are able to pattern the photoresist by conventional lithography to provide the chemical contrast required for liquids dosing by self-assembly and highly-reversible electrofluidic switching...
August 19, 2016: Materials
Ahmed A Ibrahim, Preeti Tiwari, M S Al-Assiri, A E Al-Salami, Ahmad Umar, Rajesh Kumar, S H Kim, Z A Ansari, S Baskoutas
Herein, we report a facile synthesis, characterization, and electrochemical sensing application of ZnO nanopeanuts synthesized by a simple aqueous solution process and characterized by various techniques in order to confirm the compositional, morphological, structural, crystalline phase, and optical properties of the synthesized material. The detailed characterizations revealed that the synthesized material possesses a peanut-shaped morphology, dense growth, and a wurtzite hexagonal phase along with good crystal and optical properties...
July 13, 2017: Materials
Ana Chira, Bogdan Bucur, Gabriel-Lucian Radu
Copper substrates deposed on a gold screen-printed electrode were covered with different aryl diazonium salts by electrodeposition at 0.25 mA for 30 or 300 s. Seven compounds were investigated: 4-aminophenylacetic acid, 4-aminophenethyl alcohol, 4-fluoroaniline, 4-(heptadecafluorooctyl)aniline, 4-aminoantipyrine, 4-(4-aminophenyl)butyric acid and 3,4,5-trimethoxyaniline. Quantitative monitoring of the electrodeposition process was carried out by electrogravimetry using quartz crystal microbalance (QCM). The electrodeposited mass varies between 26 ng/cm² for 4-fluoroaniline formed during 30 s to 442 ng/cm² for 4-phenylbutyric acid formed during 300 s...
February 28, 2017: Materials
Xin He, Ruihui He, Qiuming Lan, Weijie Wu, Feng Duan, Jundong Xiao, Mei Zhang, Qingguang Zeng, Jianhao Wu, Junyan Liu
A transparent and flexible film heater was fabricated; based on a hybrid structure of poly(3,4-ethylenedioxythiophene) poly(styrenesulfonate) (PEDOT:PSS) and silver nanowires (Ag NWs) using a screen printing; which is a scalable production technology. The resulting film integrates the advantages of the two conductive materials; easy film-forming and strong adhesion to the substrate of the polymer PEDOT:PSS; and high conductivity of the Ag NWs. The fabricated composite films with different NW densities exhibited the transmittance within the range from 82...
February 23, 2017: Materials
Victoria V Shumyantseva, Tatiana V Bulko, Larisa V Sigolaeva, Alexey V Kuzikov, Pavel V Pogodin, Alexander I Archakov
Electroanalysis of myoglobin (Mb) in 10 plasma samples of healthy donors (HDs) and 14 plasma samples of patients with acute myocardial infarction (AMI) was carried out with screen-printed electrodes modified first with multi-walled carbon nanotubes (MWCNT) and then with a molecularly imprinted polymer film (MIP), viz., myoglobin-imprinted electropolymerized poly(o-phenylenediamine). The differential pulse voltammetry (DPV) parameters, such as a maximum amplitude of reduction peak current (A, nA), a reduction peak area (S, nA × V), and a peak potential (P, V), were measured for the MWCNT/MIP-sensors after their incubation with non-diluted plasma...
July 20, 2017: Biosensors & Bioelectronics
Víctor Ruiz-Valdepeñas Montiel, María L Gutiérrez, Rebeca M Torrente-Rodríguez, Eloy Povedano, Eva Vargas, Á Julio Reviejo, Rosario Linacero, Francisco J Gallego, Susana Campuzano, José M Pingarrón
A novel electrochemical disposable nucleic acid biosensor for simple, rapid, and specific detection of adulterations with horsemeat is reported in this work. The biosensing platform involves immobilization of a 40-mer RNA probe specific for a characteristic fragment of the mitochondrial DNA D-loop region of horse onto the surface of magnetic microcarriers. In addition, signal amplification was accomplished by using a commercial antibody specific to RNA/DNA duplexes and a bacterial protein conjugated with a horseradish peroxidase homopolymer (ProtA-HRP40)...
August 10, 2017: Analytical Chemistry
Ricardo A G de Oliveira, Elsa María Materón, Matias Eliseo Melendez, Andre L Carvalho, Ronaldo C Faria
Breast cancer is the most common cancer in women worldwide. The detection of biomarkers has played a significant role in the early diagnosis and prognosis of breast cancer. Herein, we describe the construction of a disposable microfluidic immunoarray device (DµID) for rapid and low-cost detection of CA15-3 a protein biomarker for breast cancer. The DµID was constructed using a simple and rapid prototyping technique and was applied to detect CA15-3 in cancer patients. The DµID construction was based on a use of double-sided adhesive card with the microfluidic channel and a screen-printed array with 8 electrodes...
July 25, 2017: ACS Applied Materials & Interfaces
M Asunción Alonso-Lomillo, Olga Domínguez-Renedo, Abraham Saldaña-Botín, M Julia Arcos-Martínez
This paper shows the ability of simple modified screen-printed devices for the electrochemical detection of ascorbic acid in different samples, including serum samples. Even if the presence of uric acid does not greatly affect the analysis of ascorbic acid using screen-printed carbon electrodes modified by gold nanoparticles, dopamine must be considered an interferent. Thus, the joint determination of the three compounds has been set out using methods of multivariate calibration. The validated models, built by partial least squares regressions, have been used to determine the concentration of ascorbic acid, dopamine and uric acid of spiked serum samples, with successful results...
November 1, 2017: Talanta
Maximilian Aigner, Patricia Preissegger, Kurt Kalcher, Eda Mehmeti, Peter Macheroux, Dale Edmondson, Astrid Ortner
A novel human monoamine oxidase B (hMAO B) based biosensor for inhibitory measurements was developed. It allows both the characterisation of the type of enzyme inhibition and the sensitive and simple determination of inhibitors like selegiline hydrochloride. The sensor consists of a screen printed carbon working electrode modified with 20% manganese dioxide (MnO2) and the enzyme hMAO B, which was immobilised on the electrode via a dialysis membrane (regenerated cellulose, molecular weight cut-off 14000). Inhibition of hMAO B is evaluated by adding different concentrations of the inhibitor selegiline hydrochloride to the enzyme and applying a defined amount of the hMAO B substrate phenylethylamine (PEA)...
November 1, 2017: Talanta
Lorena Viviana Tarditto, María Alicia Zon, Hugo García Ovando, Nelio Roberto Vettorazzi, Fernando Javier Arévalo, Héctor Fernández
Diseases caused by enterotoxicogenic Escherichia coli F4 (K88) (ETEC F4) are a problem in swine production establishments. Due to the high rate of mortality and morbidity of E. coli infections, a rapid and accurate diagnosis is important in order to choose an appropriate treatment to reduce the economic impact. Therefore, an electrochemical magneto-immunosensor (EMI) was developed to detect and quantify ETEC F4 in swine feces samples through a direct non-competitive immunoassay. ETEC F4 was selectively captured by immunomagnetic separation...
November 1, 2017: Talanta
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