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Ayemeh Bagheri Hashkavayi, Jahan Bakhsh Raoof
The present work describes a label free electrochemical aptasensor for selective detection of epirubicin. In this project, 5'-thiole terminated aptamer was self-assembled on carbon screen printed electrode, which modified with electrodeposited gold nanoparticles on magnetic double-charged diazoniabicyclo [2.2.2] octane dichloride silica hybrid (Fe3O4@SiO2/DABCO) by Au-S bond. The interactions of epirubicin with aptamer on the AuNPs/Fe3O4@SiO2/DABCO/SPE have been studied by cyclic voltammetry, linear sweep voltammetry and electrochemical impedance spectroscopy...
January 14, 2017: Biosensors & Bioelectronics
W Lonsdale, D K Maurya, M Wajrak, K Alameh
The effect of contact layer on the pH sensing performance of a sputtered RuO2 thin film pH sensor is investigated. The response of pH sensors employing RuO2 thin film electrodes on screen-printed Pt, carbon and ordered mesoporous carbon (OMC) contact layers are measured over a pH range from 4 to 10. Working electrodes with OMC contact layer are found to have Nernstian pH sensitivity (-58.4mV/pH), low short-term drift rate (5.0mV/h), low hysteresis values (1.13mV) and fast reaction times (30s), after only 1h of conditioning...
March 1, 2017: Talanta
Iria Bravo, Mónica Revenga-Parra, Félix Pariente, Encarnación Lorenzo
Lactic acid is a relevant analyte in the food industry, since it affects the flavor, freshness, and storage quality of several products, such as milk and dairy products, juices, or wines. It is the product of lactose or malo-lactic fermentation. In this work, we developed a lactate biosensor based on the immobilization of lactate oxidase (LOx) onto N,N'-Bis(3,4-dihydroxybenzylidene) -1,2-diaminobenzene Schiff base tetradentate ligand-modified gold nanoparticles (3,4DHS-AuNPs) deposited onto screen-printed carbon electrodes, which exhibit a potent electrocatalytic effect towards hydrogen peroxide oxidation/reduction...
January 13, 2017: Sensors
Han Yih Lau, Haoqi Wu, Eugene J H Wee, Matt Trau, Yuling Wang, Jose R Botella
Developing quick and sensitive molecular diagnostics for plant pathogen detection is challenging. Herein, a nanoparticle based electrochemical biosensor was developed for rapid and sensitive detection of plant pathogen DNA on disposable screen-printed carbon electrodes. This 60 min assay relied on the rapid isothermal amplification of target pathogen DNA sequences by recombinase polymerase amplification (RPA) followed by gold nanoparticle-based electrochemical assessment with differential pulse voltammetry (DPV)...
January 17, 2017: Scientific Reports
Subramaniam Shankar, Raju Naveenkumar, Jeganathan Karthick, Periyasamy Mohankumar
This study focuses on the evaluation of the muscle activities associated with shoulder pain among workers of the hand screen printing (HSP) industry. Activities of three major muscles which showed higher muscle activity for a HSP job were observed for fatigue using surface electromyography (SEMG). The anatomical sites were chosen on the basis of a statistical survey and visual inspection conducted before the experiment. Activities of the deltoid, teres major and infraspinatus were recorded using SEMG and the nature of muscle activities was studied for about 50 m of cloth printing...
January 11, 2017: International Journal of Occupational Safety and Ergonomics: JOSE
Michael Benjamin, Devaraj Manoj, Kathavarayan Thenmozhi, Pundlik Rambhau Bhagat, Duraisamy Saravanakumar, Sellappan Senthilkumar
The development of efficient and cost effective nonenzymatic biosensors with remarkable sensitivity, selectivity and stability for the detection of biomolecules, especially glucose is one of the major challenges in materials- and electrochemistry. Herein, we report the design and preparation of nonenzymatic biosensor based on an ionic liquid tagged cobalt-salophen metal complex (Co-salophen-IL) immobilized on electrochemically reduced graphene oxide (ERGO) for the detection of glucose via an electrochemical oxidation...
December 30, 2016: Biosensors & Bioelectronics
Mohamed Sharafeldin, Gregory W Bishop, Snehasis Bhakta, Abdelhamid El-Sawy, Steven L Suib, James F Rusling
Ultrasensitive mediator-free electrochemical detection for biomarker proteins was achieved at low cost using a novel composite of Fe3O4 nanoparticles loaded onto graphene oxide (GO) nano-sheets (Fe3O4@GO). This paramagnetic Fe3O4@GO composite (1µm size range) was decorated with antibodies against prostate specific antigen (PSA) and prostate specific membrane antigen (PSMA), and then used to first capture these biomarkers and then deliver them to an 8-sensor detection chamber of a microfluidic immunoarray. Screen-printed carbon sensors coated with electrochemically reduced graphene oxide (ERGO) and a second set of antibodies selectively capture the biomarker-laden Fe3O4@GO particles, which subsequently catalyze hydrogen peroxide reduction to detect PSA and PSMA...
December 27, 2016: Biosensors & Bioelectronics
Hélène Guyon, François Mavré, Marjorie Catala, Serge Turcaud, Franck Brachet, Benoît Limoges, Carine Tisné, Laurent Micouin
In this work, we report an affordable, sensitive, fast and user-friendly electroanalytical method for monitoring the binding between unlabeled RNA and small compounds in microliter-size droplets using a redox-probe and disposable miniaturized screen-printed electrochemical cells.
January 5, 2017: Chemical Communications: Chem Comm
Madhu Biyani, Radhika Biyani, Tomoko Tsuchihashi, Yuzuru Takamura, Hiromi Ushijima, Eiichi Tamiya, Manish Biyani
We describe a simple and affordable "Disposable electrode printed (DEP)-On-Go" sensing platform for the rapid on-site monitoring of trace heavy metal pollutants in environmental samples for early warning by developing a mobile electrochemical device composed of palm-sized potentiostat and disposable unmodified screen-printed electrode chips. We present the analytical performance of our device for the sensitive detection of major heavy metal ions, namely, mercury, cadmium, lead, arsenic, zinc, and copper with detection limits of 1...
December 27, 2016: Sensors
Fabiana Arduini, Matteo Forchielli, Viviana Scognamiglio, Kozitsina Alisa Nikolaevna, Danila Moscone
Herein, we report a portable electrochemical biosensor based on butyrylcholinesterase (BChE) immobilized on carbon black (CB)-modified screen-printed electrodes (SPEs) for the detection of organophosphorous pesticides in olive oil. The BChE/CB-SPE biosensor was developed to detect paraoxon in standard solutions as well as in olive oil samples previously treated with the QuEChERS method to extract pesticides from the whole fatty matrix. The biosensor shows a linear concentration range of between 20 and 100 ppb for paraoxon both in standard solutions (phosphate buffer 0...
December 24, 2016: Sensors
Sun Jin Kim, Han Eol Lee, Hyeongdo Choi, Yongjun Kim, Ju Hyung We, Ji Seon Shin, Keon Jae Lee, Byung Jin Cho
Flexible thermoelectric generators (f-TEGs) are emerging as a semipermanent power source for self-powered sensors, which is an important area of research for next-generation smart network monitoring systems in the Internet-of-things era. We report in this paper a f-TEG produced by a screen-printing technique (SPT) and a laser multiscanning (LMS) lift-off process. A screen-printed TEG was fabricated on a SiO2/a-Si/quartz substrate via the SPT process, and the LMS process completely separated the rigid quartz substrate from the original TEG by selective reaction of the XeCl excimer laser with the exfoliation layer (a-Si)...
December 27, 2016: ACS Nano
Dong-Min Kim, Jong-Min Moon, Won-Chul Lee, Jang-Hee Yoon, Cheol Soo Choi, Yoon-Bo Shim
A non-enzymatic potentiometric glucose sensor for the determination of glucose in the micomolar level in saliva was developed based on a molecularly imprinted polymer (MIP) binding on a conducting polymer layer. A MIP containing acrylamide, and aminophenyl boronic acid, as a host molecule to glucose, was immobilized on benzoic acid-functionalized poly(terthiophene) (pTBA) by the amide bond formation onto a gold nanoparticles deposited-screen printed carbon electrode (pTBA/AuNPs/SPCE). Aromatic boronic acid was incorporated into the MIP layer to stably capture glucose and create a potentiometric signal through the changed pKa value of polymer film by the formation of boronate anion-glucose complex with generation of H(+) ions by the cis-diol reaction...
December 21, 2016: Biosensors & Bioelectronics
Huijie Li, Cuiling Liu, Dan Wang, Chunsun Zhang
Chemiluminescence (CL) has been widely applied in many fields, but it is rarely used in a very simple, economical but effective way. In this work, for the first time, the CL cloth-based glucose test sensors (CCGTSs) are developed as a new class of CL glucose sensors, with no need for complicated, expensive device fabrication and peripheral equipment. When integrated with desirable hydrophobic barrier in the flow channel and gravity/capillary flow induced by a difference in height between the loading zone and the detection zone, a single cloth-based device can perform the whole CL process involving two steps of enzyme reactions...
December 2, 2016: Biosensors & Bioelectronics
Charuksha Walgama, Matthew Gallman, Sadagopan Krishnan
We report single drop electroanalytical measurements of pharmaceutically and biologically relevant compounds using screen printed electrodes (SPEs) modified with carboxylated multiwalled carbon nanotubes (MWCNT-COOH) as the sensor surface. Acetaminophen, nicotine, ascorbic acid, and nicotinamide adenine dinucleotide reduced form (NADH) were detected in a single drop of solution. We show that combined polar and nonpolar interactions of analytes with -COOH functional groups and large surface area of MWCNT, respectively, allow highly sensitive analyte detection with wide dynamic range...
November 2016: Electroanalysis
Honglie Song, Junqiu Zhang, Daobing Chen, Kejun Wang, Shichao Niu, Zhiwu Han, Luquan Ren
Functional electronics has promising applications, including highly advanced human-interactive devices and healthcare monitoring. Here, we present a unique printable micron-scale cracked strain sensor (PMSCSS), which is bioinspired by a spider's crack-shaped lyriform slit organ. The PMSCSS is fabricated by a facile process that utilizes screen-printing to coat carbon black (CB) ink onto a paper substrate. With a certain bending radius, a cracked morphology emerged on the solidified ink layer. The working principle of the PMSCSS is prominently attributed to the strain-dependent variation in resistance due to the reconnection-disconnection of the crack fracture surfaces...
January 19, 2017: Nanoscale
Shimaa Eissa, Mohammed Zourob
Food allergy has increased rapidly in recent years affecting millions of people worldwide. With the increased consumption of packed food nowadays, a sensitive, accurate and rapid screening method for potential food allergens has become an urgent need in order to protect the sensitive consumers from life-threatening reactions. The current detection methods for food allergens are mostly based on immunoassays which are costly and times-consuming. In this work, we developed an aptamer/graphene-based electrochemical biosensor for on-step, sensitive and low cost detection of β-lactoglobulin (β-LG) milk protein, one of the most common food allergens specially in infants...
December 10, 2016: Biosensors & Bioelectronics
Jong-Min Moon, Dong-Min Kim, Moo Hyun Kim, Jin-Yeong Han, Dong-Keun Jung, Yoon-Bo Shim
A disposable microfluidic amperometric dual-sensor was developed for the detection of glycated hemoglobin (HbA1C) and total hemoglobin (Hb), separately, in a finger prick blood sample. The accurate level of total Hb was determined through the measurements of the cathodic currents of total Hb catalyzed by a toluidine blue O (TBO)-modified working electrode. Subsequently, after washing unbound Hb in the fluidic channel of dual sensor with PBS, the cathodic current by only HbA1C captured on aptamer was monitored using another aptamer/TBO-modified working electrode in the channel...
December 15, 2016: Biosensors & Bioelectronics
M Zouari, S Campuzano, J M Pingarrón, N Raouafi
A new method for the detection of miRNAs making use of a competitive RNA/RNA hybridization configuration is described in this work. A biotinylated miRNA (biotin-miRNA) of identical sequence to that of the target miRNA is mixed with the samples to be analyzed allowing competition to be accomplished with the target miRNA for a thiolated RNA probe assembled onto a gold nanoparticles (AuNPs) modified screen-printed electrode. After labeling the hybridized biotin-miRNA with streptavidin-HRP conjugates, amperometric detection at -0...
December 13, 2016: Biosensors & Bioelectronics
Benoît Chantemesse, Laetitia Betelli, Sébastien Solanas, Fabienne Vienney, Loïc Bollache, Alain Hartmann, Murielle Rochelet
A sensitive and inexpensive amperometric assay based on the electrochemical detection of the β-lactamase activity using the nitrocefin as substrate was developed for the rapid and quantitative detection of extended spectrum beta-lactamase-producing Escherichia coli (ESBL-EC) in urban wastewaters. The specific detection of ESBL-EC was achieved by culturing the filtered sample in a medium containing the cefotaxime supplemented or not with the potassium clavulanate inhibitor. This step was followed by the incubation of each subculture filtrate with the nitrocefin substrate which hydrolysis was monitored by amperometry using disposable carbon screen-printed sensors...
February 1, 2017: Water Research
Xinzhou Wu, Shuangshuang Shao, Zheng Chen, Zheng Cui
Copper (Cu) films and circuits were fabricated by screen-printing Cu nanoink on low-Tg (glass transition temperature) flexible plastic substrates (PEN and PET) instead of widely used high-Tg polyimide (PI) substrate. Photonic sintering of printed Cu films was carried out using intensive pulsed light (IPL). Low resistivities of 28 μΩ · cm on PEN and 44 μΩ · cm on PET were obtained without damaging the substrates. The sintered Cu films exhibited strong adhesion to PEN and PET substrates, with measured adhesion strength of 5B by the ASTM D3359 international standard, whereas the top part of the copper film on the PI substrate was stripped off during the adhesion test...
January 20, 2017: Nanotechnology
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