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https://www.readbyqxmd.com/read/29777816/aptamer-based-assay-for-monitoring-genetic-disorder-phenylketonuria-pku
#1
Mohammad Hasanzadeh, Amir Zargami, Hossein Navay Baghban, Ahad Mokhtarzadeh, Nasrin Shadjou, Soltanali Mahboob
The genetic disorder phenylketonuria (PKU) is the inability to metabolize phenylalanine because of a lack of the enzyme phenylalanine hydroxylase. Phenylalanine is used to biochemically form proteins, coded for by DNA. The development of an apta-assay for detection of l-Phenylalanine is presented in this work. A highly specific DNA-aptamer, selected to l-Phenylalanine was immobilized onto a gold nanostructure and electrochemical measurements were performed in a solution containing the phosphate buffer solution with physiological pH...
May 16, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29777681/electrochemical-enzymatic-fenitrothion-sensor-based-on-a-tyrosinase-poly-2-hydroxybenzamide-modified-graphite-electrode
#2
Maria de Fátima Alves, Ricardo Augusto Moreira de Souza Corrêa, Filipe Soares da Cruz, Diego Leoni Franco, Lucas Franco Ferreira
This paper reports the electrosynthesis and characterisation of a polymeric film derived from 2-hydroxybenzamide over a graphite electrode and its application as an enzymatic biosensor for the determination and quantification of the pesticide fenitrothion. The material was analysed by scanning electron microscopy and its electrochemical properties characterised by cyclic voltammetry and electrochemical impedance spectroscopy. The enzyme tyrosinase was immobilised over the modified electrode by the drop and dry technique...
May 16, 2018: Analytical Biochemistry
https://www.readbyqxmd.com/read/29776549/in-situ-growth-of-iron-based-metal-organic-framework-crystal-on-ordered-mesoporous-carbon-for-efficient-electrocatalysis-of-p-nitrotoluene-and-hydrazine
#3
Shuang Yuan, Xiangjie Bo, Liping Guo
In this paper, H2 N-Fe-MIL-88B@OMC composites were fabricated by the simple hydrothermal method through in-situ growth iron-based metal-organic frameworks (H2 N-Fe-MIL-88B) on ordered mesoporous carbon (OMC). Compared with pure H2 N-Fe-MIL-88B crystals, the introduction of OMC can shrink the size of H2 N-Fe-MIL-88B, increase the specific surface area, decrease the electron transfer resistance and drastically improve the electrochemical performance of H2 N-Fe-MIL-88B@OMC. The composite exhibits remarkable electrocatalytic activity for p-nitrotoluene (p-NT) reduction and hydrazine (N2 H4 ) oxidation...
September 18, 2018: Analytica Chimica Acta
https://www.readbyqxmd.com/read/29775856/a-simple-ultrasensitive-electrochemical-sensor-for-simultaneous-determination-of-gallic-acid-and-uric-acid-in-human-urine-and-fruit-juices-based-on-zirconia-choline-chloride-gold-nanoparticles-modified-carbon-paste-electrode
#4
Seyed Alireza Shahamirifard, Mehrorang Ghaedi, Zahra Razmi, Shaaker Hajati
The determination of gallic acid (GA) and uric acid (UA) is essential due to their biological properties. Numerous methods have been reported for the analysis of GA and UA in various real samples. However, the development of a simple, rapid and practical sensor still remains a great challenge. Here, a carbon paste electrode (CPE) was modified by nanocomposite containing zirconia nanoparticles (ZrO2 NPs), Choline chloride (ChCl) and gold nanoparticles (AuNPs) to construct ZrO2 -ChCl-AuNPs/CPE as electrochemical sensor for the simultaneous electro-oxidation of GA and UA...
May 8, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29775720/diagnosing-human-blood-clotting-deficiency
#5
REVIEW
Chong Cheen Ong, Subash C B Gopinath, Leong Wei Xian Rebecca, Veeradasan Perumal, Thangavel Lakshmipriya, Mohamed Shuaib Mohamed Saheed
There are different clotting factors present in blood, carries the clotting cascade and excessive bleeding may cause a deficiency in the clotting. Diagnosis of deficiency in clotting reduces the potential fatality. For enabling a sensor to detect the clotting factors, suitable probes such as antibody and aptamer have been used to capture the clotting factors on the sensing surface. Two major clotting factors were widely studied in detail for the diagnosis of clotting deficiency, which includes factor IX and thrombin...
May 15, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29774341/porous-nico-2-o-4-nanoarray-integrated-binder-free-3d-open-electrode-offers-a-highly-efficient-sensing-platform-for-enzyme-free-glucose-detection
#6
X Luo, M Huang, D He, M Wang, Y Zhang, P Jiang
High electrical conductivity and the exposure to more active sites are crucial to boost the performance of a glucose sensor. A porous binary metal oxide nanoarray integrated on a binder-free 3D electrode is expected to offer a highly sensitive sensing platform. As a model, porous NiCo2O4 nanowire arrays supported on carbon cloth (NiCo2O4 NWA/CC) have been prepared and used for enzyme-free glucose sensing. NiCo2O4 NWA/CC shows larger effective surface area, superior electronic conductivity, and higher catalytic activity towards enzyme-free glucose sensing, with a linear range from 1 μM to 0...
May 18, 2018: Analyst
https://www.readbyqxmd.com/read/29772383/a-signal-decreased-electrochemical-immunosensor-for-the-sensitive-detection-of-lag-3-protein-based-on-a-hollow-nanobox-mofs-aupt-alloy
#7
Wei Xu, Zhen Qin, Yutong Hao, Qiang He, Shuai Chen, Zhongshuang Zhang, Dan Peng, Haiyan Wen, Jun Chen, Jingfu Qiu, Chaorui Li
In this work, hollow nanobox metal-organic framework (HNM) nanocomposites were synthesised and utilised for the first time in a signal decreased electrochemical immunosensor for the ultrasensitive quantitative determination of lymphocyte activation gene-3 (LAG-3) protein, which is a newly discovered biomarker. With the aid of signal materials, namely, SiO2 -tagged anti-LAG-3 antibody (SiO2 -Ab2 ) and the biotin-streptavidin system, the sensor can achieve signal amplification. Encapsulation of tin dioxide-functionalised reduced graphene oxide (rGO-SnO2 ) and gold and platinum alloys (AuPt alloys) onto the surface of hollow nanobox metal-organic frameworks (MOFs) was performed to prepare rGO-SnO2 /hollow nanobox-MOFs/AuPt alloys (rGO-SnO2 /HNMs/AuPt) as the matrix...
May 8, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29770694/detection-of-pcr-amplified-tuberculosis-dna-fragments-with-polyelectrolyte-modified-field-effect-sensors
#8
Thomas Stefan Bronder, Max Philipp Jessing, Arshak Poghossian, Michael Keusgen, Michael J Schöning
Field-effect-based electrolyte-insulator-semiconductor (EIS) sensors were modified with a bilayer of positively charged weak polyelectrolyte (poly(allylamine hydrochloride) (PAH)) and probe single-stranded deoxyribonucleic acid (ssDNA) and are used for the detection of target complementary deoxyribonucleic acid (cDNA) in different test solutions. The sensing mechanism is based on the detection of the intrinsic molecular charge of target cDNA molecules after the hybridization event between cDNA and immobilized probe ssDNA...
May 17, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29768812/sensitive-detection-of-rifampicin-based-on-au-carbon-nanocomposite
#9
Ruimin Xing, Xiaoyu Zhao, Yanli Xu, Haitang Yang, Zhixian Chang, Jianying Qu, Xiuhua Liu, Jing-He Yang, Shanhu Liu
Gold nanoparticles-supported Cabot Vulcan XC72R (Au/VXC72R) nanocomposite was synthesized by chemical reduction of gold (III) chloride with VXC72R. A novel electrochemical sensor based on the Au/VXC72R nanocomposite has been fabricated for the sensitive detection of rifampicin (RIF). Field-emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), X-ray powder diffractometer (XRD) and X-ray photoelectron spectroscopy (XPS) were used to characterize the morphology, structure and compositions of the nanocomposite...
January 1, 2018: Journal of Nanoscience and Nanotechnology
https://www.readbyqxmd.com/read/29767947/application-of-calcium-binding-motif-of-e-cadherin-for-electrochemical-detection-of-pb-ii
#10
Hamid R Lotfi Zadeh Zhad, Rebecca Y Lai
We report, for the first time, the use of a 13-amino-acid peptide sequence derived from the calcium-binding site of E-cadherin in the fabrication of an electrochemical peptide-based (E-PB) Pb(II) sensor. The sensing mechanism is analogous to that of previously developed E-PB sensors. Binding of Pb(II) rigidifies the surface-immobilized and methylene blue (MB)-modified peptide probe, thereby limiting the accessibility of the tethered MB to the electrode surface. This change in probe flexibility results in a reduction in the MB current that is dependent on the target concentration...
May 16, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29767510/non-enzymatic-wearable-sensor-for-electrochemical-analysis-of-perspiration-glucose
#11
Xiaofei Zhu, Yinhui Ju, Jian Chen, Deye Liu, Hong Liu
We report a non-enzymatic wearable sensor for electrochemical analysis of perspiration glucose. Multi-potential steps are applied on a Au electrode, including a high negative pretreatment potential step for proton reduction which produc-es a localized alkaline condition, a moderate potential step for electrocatalytic oxidation of glucose under the alkaline condi-tion, and a positive potential step to clean and reactivate the electrode surface for the next detection. Fluorocarbon-based materials were coated on the Au electrode for improving the selectivity and robustness of the sensor...
May 16, 2018: ACS Sensors
https://www.readbyqxmd.com/read/29765797/electrodeposition-of-reduced-graphene-oxide-with-chitosan-based-on-the-coordination-deposition-method
#12
Mingyang Liu, Yanjun Chen, Chaoran Qin, Zheng Zhang, Shuai Ma, Xiuru Cai, Xueqian Li, Yifeng Wang
The electrodeposition of graphene has drawn considerable attention due to its appealing applications for sensors, supercapacitors and lithium-ion batteries. However, there are still some limitations in the current electrodeposition methods for graphene. Here, we present a novel electrodeposition method for the direct deposition of reduced graphene oxide (rGO) with chitosan. In this method, a 2-hydroxypropyltrimethylammonium chloride-based chitosan-modified rGO material was prepared. This material disperses homogenously in the chitosan solution, forming a deposition solution with good dispersion stability...
2018: Beilstein Journal of Nanotechnology
https://www.readbyqxmd.com/read/29762527/self-assembled-ag-cu%C3%A2-o-nanocomposite-films-at-air-liquid-interfaces-for-surface-enhanced-raman-scattering-and-electrochemical-detection-of-h%C3%A2-o%C3%A2
#13
Li Wang, Huan Qi, Lei Chen, Yantao Sun, Zhuang Li
We employ a facile and novel route to synthesize multifunctional Ag-Cu₂O nanocomposite films through the self-assembly of nanoparticles at an air-liquid interface. In the ethanol-water phase, AgNO₃ and Cu(NO₃)₂ were reduced to Ag-Cu₂O nanoparticles by NaBH₄ in the presence of cinnamic acid. The Ag-Cu₂O nanoparticles were immediately trapped at the air-liquid interface to form two-dimensional nanocomposite films after the reduction reaction was finished. The morphology of the nanocomposite films could be controlled by the systematic regulation of experimental parameters...
May 15, 2018: Nanomaterials
https://www.readbyqxmd.com/read/29762494/a-facile-electrochemical-sensor-based-on-pyts%C3%A2-cnts-for-simultaneous-determination-of-cadmium-and-lead-ions
#14
Ruyuan Jiang, Niantao Liu, Sanshuang Gao, Xamxikamar Mamat, Yuhong Su, Thomas Wagberg, Yongtao Li, Xun Hu, Guangzhi Hu
A simple and easy method was implemented for the contemporary detection of cadmium (Cd2+ ) and lead (Pb2+ ) ions using 1,3,6,8-pyrenetetrasulfonic acid sodium salt-functionalized carbon nanotubes nanocomposites (PyTS⁻CNTs). The morphology and composition of the obtained PyTS⁻CNTs were characterized using scanning electron microscopy (SEM), energy dispersive spectrometry (EDS), and X-ray photoelectron spectroscopy (XPS). The experimental results confirmed that the fabricated PyTS⁻CNTs exhibited good selectivity and sensitivity for metal ion-sensing owing to the insertion of sulfonic acid groups...
May 15, 2018: Sensors
https://www.readbyqxmd.com/read/29762016/rapid-2-ms-interrogation-of-electrochemical-aptamer-based-sensor-response-using-intermittent-pulse-amperometry
#15
Mirelis Santos-Cancel, Robert Alexander Lazenby, Ryan J White
In this manuscript, we employ the technique intermittent pulse amperometry (IPA) to interrogate equilibrium and kinetic target binding to the surface of electrochemical, aptamer-based (E-AB) sensors, achieving as fast as 2 ms time resolution. E-AB sensors comprise an electrode surface modified with a flexible nucleic acid aptamer tethered at the 3'-terminus with a redox-active molecule. The introduction of a target changes the conformation and flexibility of the nucleic acid which alters the charge transfer rate of the appended redox molecule...
May 15, 2018: ACS Sensors
https://www.readbyqxmd.com/read/29761564/laser-induced-molybdenum-carbide-graphene-composites-for-3d-foldable-paper-electronics
#16
Xining Zang, Caiwei Shen, Yao Chu, Buxuan Li, Minsong Wei, Junwen Zhong, Mohan Sanghadasa, Liwei Lin
Versatile and low-cost manufacturing processes/materials are essential for the development of paper electronics. Here, a direct-write laser patterning process is developed to make conductive molybdenum carbide-graphene (MCG) composites directly on paper substrates. The hierarchically porous MCG structures are converted from fibrous paper soaked with the gelatin-mediated inks containing molybdenum ions. The resulting Mo3 C2 and graphene composites are mechanically stable and electrochemically active for various potential applications, such as electrochemical ion detectors and gas sensors, energy harvesters, and supercapacitors...
May 15, 2018: Advanced Materials
https://www.readbyqxmd.com/read/29759240/reduced-graphene-oxide-zno-nanocomposite-based-electrochemical-sensor-for-sensitive-and-selective-monitoring-of-8-hydroxy-2-deoxyguanosine
#17
Junxing Hao, Kangbing Wu, Chidan Wan, Yong Tang
Developing reliable and feasible electrochemical sensors for the detection of 8-hydroxy-2'-deoxyguanosine (8-OHdG) is important because the urinary level of 8-OHdG is related to cancer disease. Moreover, the co-existed uric acid (UA) as an interference severe affects the sensitive detection of 8-OHdG. Herein, sensitive monitoring of 8-OHdG was conducted using a nanocomposite of reduced graphene oxide (rGO) and ZnO nanoparticles (ZnO@rGO) as the sensing material. This nanocomposite was prepared via in-situ reduction of GO with Zn powders...
August 1, 2018: Talanta
https://www.readbyqxmd.com/read/29759227/a-novel-electrochemical-sensor-for-detecting-hyperin-with-a-nanocomposite-of-zro-2-sds-swcnts-as-decoration
#18
Shuo Li, Sheng Lei, Qian Yu, Lina Zou, Baoxian Ye
A novel high-sensitive electrochemical sensor with glassy carbon electrode (GCE) as support for hyperin determination is successfully designed and constructed, and the well-shaped nano-meter modified material is synthesized via a one-step and facile route. Functionalized with surfactant sodium dodecyl sulfate (SDS), Single-Walled Carbon Nanotubes (SWCNTs) are synchronously grafted with ZrO2 nanoparticles to develop into the as-prepared nano-composite (ZrO2 -SDS-SWCNTs). Compared to the previous reports related with hyperin detection, the linear range gets wider and detection limit (LOD) becomes lower with the aid of this novel nano-composite modified glassy carbon electrode (ZrO2 -SDS-SWCNTs/GCE)...
August 1, 2018: Talanta
https://www.readbyqxmd.com/read/29759210/smart-bi-metallic-perovskite-nanofibers-as-selective-and-reusable-sensors-of-nano-level-concentrations-of-non-steroidal-anti-inflammatory-drugs
#19
Mona A Mohamed, Menna M Hasan, Ibrahim H Abdullah, Ahmed M Abdellah, Ali M Yehia, Nashaat Ahmed, Walaa Abbas, Nageh K Allam
A strategy for trace-level carbon-based electrochemical sensors is investigated via exploring the interesting properties of BaNb2 O6 nanofibers (NFs). Utilizing adsorptive stripping square wave voltammetry (ASSWV), an electrochemical sensing platform was developed based on BaNb2 O6 nanofibers-modified carbon paste electrode (CPE) for the sensitive detection of lornoxicam (LOR). Different techniques were used to characterize the fabricated BaNb2 O6 perovskite NFs. The obtained data show the feasibility to electro-oxidize LOR and paracetamol (PAR) on the surface of the fabricated sensor...
August 1, 2018: Talanta
https://www.readbyqxmd.com/read/29759209/fabrication-of-rgo-nico-2-o-4-nanorods-composite-from-deep-eutectic-solvents-for-nonenzymatic-amperometric-sensing-of-glucose
#20
Yue Ni, Jian Xu, Hong Liu, Shijun Shao
A novel reduced graphene oxide supported nickel cobaltate nanorods composite (RGO-NiCo2 O4 ) was prepared by a simple ionothermal method in deep eutectic solvents for the first time. Electrochemical results demonstrated that the obtained nanocomposite modified glassy carbon electrode exhibited excellent electrocatalytic performance towards the oxidation of glucose with a wide double-linear range from 1 μM to 25 mM and a low detection limit of 0.35 μM (S/N = 3). NiCo2 O4 nanorods with many small interconnected nanoparticles provided many electrocatalytic active sites, while RGO with large surface area offered good electrical conductivity...
August 1, 2018: Talanta
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