keyword
MENU ▼
Read by QxMD icon Read
search

Clinical assay

keyword
https://www.readbyqxmd.com/read/29475000/quantitation-of-25-hydroxyvitamin-d-in-dried-blood-spots-by-2d-lc-ms-ms-without-derivatization-and-correlation-with-serum-in-adult-and-pediatric-studies
#1
Berit P Jensen, Rajneeta Saraf, Jing Ma, Sarah Berry, Cameron C Grant, Carlos A Camargo, Christiaan W Sies
BACKGROUND: Demand for measurement of 25-hydroxyvitamin D (25OHD) is growing and dried blood spot (DBS) sampling is attractive as samples are easier to collect, transport and store. METHODS: A 2D LC-MS/MS assay without derivatization was developed. DBS punches (3.2 mm) were ultrasonicated with d6 -25OHD3 in 70% methanol followed by hexane extraction, dry-down and reconstitution. The assay was validated and applied to two studies comparing whole blood adult DBS with serum samples (n = 40) and neonatal whole blood DBS with cord serum samples (n = 80)...
February 20, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/29474986/recommendations-for-clinical-cyp2c19-genotyping-allele-selection-a-report-of-the-association-for-molecular-pathology
#2
REVIEW
Victoria M Pratt, Andria L Del Tredici, Houda Hachad, Yuan Ji, Lisa V Kalman, Stuart A Scott, Karen E Weck
This document was developed by the Pharmacogenetics (PGx) Working Group of the Association for Molecular Pathology (AMP) Clinical Practice Committee, whose aim is to recommend variants for inclusion in clinical pharmacogenetic testing panels. The goals of the AMP PGx Working Group are to define the key attributes of PGx alleles recommended for clinical testing, and to define a minimum set of variants that should be included in clinical PGx genotyping assays. These recommendations include a minimum panel of variant alleles (Tier 1) and an extended panel of variant alleles (Tier 2) that will aid clinical laboratories when designing PGx assays...
February 20, 2018: Journal of Molecular Diagnostics: JMD
https://www.readbyqxmd.com/read/29474983/an-in-depth-evaluation-of-the-validity-and-logistics-surrounding-the-testing-of-ar-v7-mrna-expression-in-circulating-tumor-cells
#3
Anieta M Sieuwerts, Bianca Mostert, Michelle van der Vlugt-Daane, Jaco Kraan, Corine Beaufort, Mai Van, Wendy J C Prager, Bram De Laere, Nick Beije, Paul Hamberg, Hans M Westgeest, Metin Tascilar, Luc Y Dirix, Wendy Onstenk, Ronald de Wit, Martijn P Lolkema, Ron H J Mathijssen, John W M Martens, Stefan Sleijfer
Recent reports have emphasized the clinical relevance of detecting the androgen receptor splice variant 7 (AR-V7) in circulating tumor cells (CTCs). Our aim was to set up a validated multicenter pipeline to measure AR-V7 by RT-qPCR in RNA isolated from CellSearch-enriched CTCs to provide an AR-V7 positive/negative score in a clinically acceptable time range. CellSearch-enirched CTCs from metastatic castration-resistant prostate cancer patients were characterized by RT-qPCR. After optimization it was prospectively tested whether it was possible to report the AR-V7 status within 11 days (PRELUDE study)...
February 20, 2018: Journal of Molecular Diagnostics: JMD
https://www.readbyqxmd.com/read/29474420/a-new-high-performance-liquid-chromatography-tandem-mass-spectrometry-method-for-the-determination-of-paclitaxel-and-6%C3%AE-hydroxy-paclitaxel-in-human-plasma-development-validation-and-application-in-a-clinical-pharmacokinetic-study
#4
Bianca Posocco, Mauro Buzzo, Andrea Follegot, Luciana Giodini, Roberto Sorio, Elena Marangon, Giuseppe Toffoli
Paclitaxel belongs to the taxanes family and it is used, alone or in multidrug regimens, for the therapy of several solid tumours, such as breast-, lung-, head and neck-, and ovarian cancer. Standard dosing of chemotherapy does not take into account the many inter-patient differences that make drug exposure highly variable, thus leading to the insurgence of severe toxicity. This is particularly true for paclitaxel considering that a relationship between haematological toxicity and plasma exposure was found...
2018: PloS One
https://www.readbyqxmd.com/read/29474357/effects-of-flow-rate-on-the-migration-of-different-plasticizers-from-pvc-infusion-medical-devices
#5
Lise Bernard, Teuta Eljezi, Hélène Clauson, Céline Lambert, Yassine Bouattour, Philip Chennell, Bruno Pereira, Valérie Sautou
Infusion medical devices (MDs) used in hospitals are often made of plasticized polyvinylchloride (PVC). These plasticizers may leach out into infused solutions during clinical practice, especially during risk-situations, e.g multiple infusions in Intensive Care Units and thus may enter into contact with the patients. The migrability of the plasticizers is dependent of several clinical parameters such as temperature, contact time, nature of the simulant, etc… However, no data is available about the influence of the flow rate at which drug solutions are administrated...
2018: PloS One
https://www.readbyqxmd.com/read/29474322/validation-of-a-novel-molecular-host-response-assay-to-diagnose-infection-in-hospitalized-patients-admitted-to-the-icu-with-acute-respiratory-failure
#6
Maria E Koster-Brouwer, Diana M Verboom, Brendon P Scicluna, Kirsten van de Groep, Jos F Frencken, Davy Janssen, Rob Schuurman, Marcus J Schultz, Tom van der Poll, Marc J M Bonten, Olaf L Cremer
OBJECTIVES: Discrimination between infectious and noninfectious causes of acute respiratory failure is difficult in patients admitted to the ICU after a period of hospitalization. Using a novel biomarker test (SeptiCyte LAB), we aimed to distinguish between infection and inflammation in this population. DESIGN: Nested cohort study. SETTING: Two tertiary mixed ICUs in the Netherlands. PATIENTS: Hospitalized patients with acute respiratory failure requiring mechanical ventilation upon ICU admission from 2011 to 2013...
March 2018: Critical Care Medicine
https://www.readbyqxmd.com/read/29474275/development-and-validation-of-a-simultaneous-quantification-method-of-ruxolitinib-vismodegib-olaparib-and-pazopanib-in-human-plasma-using-lc-ms-ms
#7
Claire Pressiat, Huu-Hien Huynh, Alain Plé, Hélène Sauvageon, Isabelle Madelaine, Cécile Chougnet, Christine Le Maignan, Samia Mourah, Lauriane Goldwirt
BACKGROUND: A simple, rapid, and sensitive LC-MS/MS method has been developed and validated for the quantification of ruxolitinib, olaparib, vismodegib, and pazopanib in human plasma. METHODS: After a simple protein precipitation of plasma samples, the chromatographic separation was performed using an UPLC system coupled with MS/MS in a positive ionization mode. The mobile phase consisted of a gradient elution of 10 mmol/L formate ammonium buffer containing 0.1% (v/v) formic acid (phase A) and acetonitrile with 0...
February 22, 2018: Therapeutic Drug Monitoring
https://www.readbyqxmd.com/read/29473983/biofilm-inhibitory-efficiency-of-phytol-in-combination-with-cefotaxime-against-nosocomial-pathogen-acinetobacter-baumannii
#8
Srinivasan Ramanathan, Kannappan Arunachalam, Sivasankar Chandran, Rathika Selvaraj, Karutha Pandian Shunmugiah, Veera Ravi Arumugam
AIMS: This study aimed to evaluate the antibiofilm potential of phytol and cefotaxime combinations (PCCs) against A. baumannii and to elucidate the molecular mechanism of their antibiofilm potential through the transcriptomic approach. METHODS AND RESULTS: Phytol and cefotaxime combination(s) (PCC(s) [160 μg ml-1 + 8 μg ml-1 for microbial type culture collection (MTCC) strain and 160 μg ml-1 + 0.5 μg ml-1 for clinical isolate] effectively inhibited the A. baumannii biofilm formation...
February 23, 2018: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29473705/alpha-1-antitrypsin-treatment-of-new-onset-type-1-diabetes-an-open-label-phase-i-clinical-trial-retain-to-assess-safety-and-pharmacokinetics
#9
Gordon C Weir, Mario R Ehlers, Kristina M Harris, Sai Kanaparthi, Alice Long, Deborah Phippard, Lia J Weiner, Brett Jepson, James G McNamara, Maria Koulmanda, Terry B Strom
OBJECTIVE: To determine the safety and pharmacokinetics of alpha-1 antitrypsin (AAT) in adults and children. RESEARCH DESIGN AND METHODS: Short term AAT treatment restores euglycemia in the non-obese mouse model of type 1 diabetes. A phase I multicenter study in 16 subjects with new-onset type 1 diabetes studied the safety and pharmacokinetics of Aralast NPTM (AAT). This open-label, dose-escalation study enrolled eight adults ages 16-35 and eight children ages 8-15 within 100 days of diagnosis, to receive 12 infusions of AAT: a low dose of 45 mg/kg weekly for six weeks, followed by a higher dose of 90 mg/kg for six weeks...
February 23, 2018: Pediatric Diabetes
https://www.readbyqxmd.com/read/29473694/comparing-apples-and-oranges-and-pears-and-kumquats-the-misuse-of-index-systems-for-processing-histopathology-data-in-fish-toxicological-bioassays
#10
Jeffrey C Wolf
Histopathology index systems involve the application of weighted scores to various diagnostic findings for the purpose of calculating overall organ and/or whole animal health measurements. Such systems were originally developed as tools for monitoring the general health of fish populations. More recently, index systems have been applied to hypothesis-based toxicological studies, the goal of which is to investigate whether a cause-and-effect relationship exists between exposure to a particular test substance or environmental contaminant and morphologic effects in fish tissues...
February 23, 2018: Environmental Toxicology and Chemistry
https://www.readbyqxmd.com/read/29473487/new-design-development-and-optimization-of-an-in-house-quantitative-taqman-real-time-pcr-assay-for-hiv-1-viral-load-measurement
#11
Hassan Noorbazargan, Seyed Alireza Nadji, Siamak Mirab Samiee, Mahdi Paryan, Samira Mohammadi-Yeganeh
Background Viral load measurement is commonly applicable to monitor HIV infection in patients to determine the number of HIV-RNA in serum samples of individuals. The aim of the present study was to set up a highly specific, sensitive, and reproducible home-brewed Real-time PCR assay based on TaqMan chemistry to quantify HIV-1 RNA genome. Methods In this study, three sets of primer pairs and a TaqMan probe were designed for HIV subtypes conserved sequences. An internal control was included in this assay to evaluate the presence of inhibition...
February 23, 2018: HIV Clinical Trials
https://www.readbyqxmd.com/read/29473327/epidemiological-investigation-of-pseudorabies-in-shandong-province-from-2013-to-2016
#12
J Gu, D Hu, T Peng, Y Wang, Z Ma, Z Liu, F Meng, Y Shang, S Liu, Y Xiao
In late 2011, a variant pseudorabies virus (vPRV) emerged in Bartha-K61-vaccinated pig herds, resulting in high morbidity and mortality of piglets in China. Since 2013, the autopsy lesions, histological examinations, virus isolation, phylogenetic analysis and selection pressure analysis of the gE gene of vPRV were recorded for 395 clinical cases, and 5,033 pig serum samples were detected by PRV gE-coated enzyme-linked immunosorbent assay. The major clinical symptoms were abortion in pregnant sows, fatal neurological signs in piglets and respiratory disease in growing pigs...
February 22, 2018: Transboundary and Emerging Diseases
https://www.readbyqxmd.com/read/29473183/peritoneal-dialysis-fluid-induced-fragmentation-of-golgi-apparatus-as-a-biocompatibility-marker
#13
Masanao Iwamoto, Alice Okazaki, Sayaka Murata, Masaki Hirukawa, Keiichi Miyamoto, Tomohiro Murata, Eiji Ishikawa, Toshimichi Yoshida, Takashi Horiuchi
In vitro biocompatibility assessments that consider physiologically appropriate conditions of cell exposure to peritoneal dialysis fluids (PDFs) are still awaited. In this study, we found that fragmentation of Golgi apparatus occurred in a pH-dependent manner within 30-min exposure to five distinct commercially available PDFs, which showed no marked difference in their effects on cell viability in the conventional MTT assay. Fluorescence microscopy analysis of labeling antibody against cis-Golgi protein GM130 indicated that the stacked cisternal structure was maintained in the perinuclear area of both M199 culture medium and a neutral-pH PDF groups...
February 23, 2018: Artificial Organs
https://www.readbyqxmd.com/read/29473173/ultraviolet-light-based-pathogen-inactivation-and-alloimmunization-after-platelet-transfusion-results-from-a-randomized-trial
#14
Philip J Norris, Zhanna Kaidarova, Erica Maiorana, Silvano Milani, Mila Lebedeva, Michael P Busch, Brian Custer, Paolo Rebulla
BACKGROUND: The current study explored whether pathogen-reduction treatment of platelet components before transfusion would decrease the risk of alloimmunization. STUDY DESIGN AND METHODS: Study participants were patients with hematologic cancer who were included in two parallel, randomized clinical trials testing pathogen-reduction treatment versus conventional platelets using the Mirasol or Intercept pathogen-reduction systems. Patients who had a baseline, pretransfusion sample and a follow-up, posttransfusion sample were included in the study (n = 179 patients in each study arm)...
February 22, 2018: Transfusion
https://www.readbyqxmd.com/read/29473062/an-ultrasensitive-microchip-electrophoresis-assay-based-on-separation-assisted-double-cycling-signal-amplification-strategy-for-microrna-detection-in-cell-lysate
#15
Kaiji Wei, Jingjin Zhao, Xiaoshu Luo, Senghui Qiu, Fuyun He, Shuting Li, Shulin Zhao
Microchip electrophoresis (MCE) assay is an analysis technique with low consumption and high automation. It is a useful tool in biomedical research and clinical diagnosis. However, the low detection sensitivity limits its application in trace biomarker analysis because of its extremely small sample size. To address the need for high sensitivity in MCE, we have developed an ultrasensitive MCE method based on a separation-assisted double cycling signal amplification strategy for the detection of microRNA (miRNA) in cell lysate...
February 23, 2018: Analyst
https://www.readbyqxmd.com/read/29473029/fluoxetine-administration-in-juvenile-monkeys-implications-for-pharmacotherapy-in-children
#16
REVIEW
Mari S Golub, Casey E Hogrefe, Richard J Sherwood, Christoph W Turck
Fluoxetine therapy has been approved for children with major depressive disorder and obsessive compulsive disorder for over 14 years and has expanded to other childhood behavior disorders. As use increases, more detail on fluoxetine effects during juvenile brain development can help maintain safe and effective use of this therapy. Here, a narrative review is provided of previously published findings from a large nonhuman primate project. Fluoxetine was administered to juvenile male rhesus monkeys for an extended period (2 years) prior to puberty...
2018: Frontiers in Pediatrics
https://www.readbyqxmd.com/read/29472905/1-2-4-dichlorophenethyl-amino-3-phenoxypropan-2-ol-kills-pseudomonas-aeruginosa-through-extensive-membrane-damage
#17
Valerie Defraine, Veerle Liebens, Evelien Loos, Toon Swings, Bram Weytjens, Carolina Fierro, Kathleen Marchal, Liam Sharkey, Alex J O'Neill, Romu Corbau, Arnaud Marchand, Patrick Chaltin, Maarten Fauvart, Jan Michiels
The ever increasing multidrug-resistance of clinically important pathogens and the lack of novel antibiotics have resulted in a true antibiotic crisis where many antibiotics are no longer effective. Further complicating the treatment of bacterial infections are antibiotic-tolerant persister cells. Besides being responsible for the recalcitrant nature of chronic infections, persister cells greatly contribute to the observed antibiotic tolerance in biofilms and even facilitate the emergence of antibiotic resistance...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29472772/effects-of-decitabine-on-the-proliferation-of-k562-cells-and-the-expression-of-dr4-gene
#18
Wenhui Zhang, Yuqing Chen, Xiaohang Pei, Yuzhu Zang, Shuangyin Han
Objective: To investigate the role of DR4 gene in the occurrence, development and prognosis of acute myeloid leukemia (AML), find a new regulatory gene of Decitabine for the treatment of AML, namely DR4 gene, and explore the molecular mechanism of AML in the treatment of AML. Methods: The methylation level and the mRNA expression level of DR4 gene promoters of bone marrow mononuclear cells in 122 patients with newly diagnosed AML and 24 patients with iron deficiency anemia (IDA) were detected using Methylation specific PCR (MS-PCR) and Q-RT-PCR, respectively, and a correlation analysis of them was conducted...
February 2018: Saudi Journal of Biological Sciences
https://www.readbyqxmd.com/read/29472753/autopsy-proven-mirtazapine-withdrawal-induced-mania-hypomania-associated-with-sudden-death
#19
Rena Pombo, Etta Johnson, Alejandra Gamboa, Bennet Omalu
Manic episodes induced by antidepressant withdrawal are rarely reported. Mirtazapine is a tetracyclic, piperazinoazepine compound and is a noradrenergic, adrenergic, serotonergic, histaminergic, and muscarinic-antagonist antidepressant that is used for the treatment of major depression and other psychiatric illnesses. There are several reported cases of manic/hypomanic episodes induced by mirtazapine withdrawal based on suspected clinical symptoms that were not confirmed by autopsy and toxicology. We present the first reported case of mirtazapine withdrawal-induced mania/hypomania associated with sudden death and confirmed by autopsy and toxicology...
October 2017: Journal of Pharmacology & Pharmacotherapeutics
https://www.readbyqxmd.com/read/29472349/clinical-relevance-of-idh1-2-mutant-allele-burden-during-follow-up-in-acute-myeloid-leukemia-a-study-by-the-french-alfa-group
#20
Yann Ferret, Nicolas Boissel, Nathalie Helevaut, Jordan Madic, Olivier Nibourel, Alice Marceau-Renaut, Maxime Bucci, Sandrine Geffroy, Karine Celli-Lebras, Sylvie Castaigne, Xavier Thomas, Christine Terré, Hervé Dombret, Claude Preudhomme, Aline Renneville
Assessment of minimal residual disease has emerged as a powerful prognostic factor in acute myeloid leukemia. In this study, we investigated the potential of IDH1/2 mutations as targets for minimal residual disease assessment in acute myeloid leukemia, since these mutations collectively occur in 15-20% of acute myeloid leukemia and now represent druggable targets. We employed droplet digital PCR assays to quantify IDH1R132, IDH2R140, and IDH2R172 mutations on genomic DNA in 322 samples from 103 adult patients with primary IDH1/2 mutant acute myeloid leukemia and enrolled on Acute Leukemia French Association (ALFA) -0701 or -0702 clinical trials...
February 22, 2018: Haematologica
keyword
keyword
101127
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"