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https://www.readbyqxmd.com/read/29677618/altered-serum-levels-of-vascular-endothelial-growth-factor-in-first-episode-drug-na%C3%A3-ve-and-chronic-medicated-schizophrenia
#1
Fei Ye, Qiongqiong Zhan, Wenhuan Xiao, Xiaowei Tang, Jin Li, Hui Dong, Weiwei Sha, Xiaobin Zhang
There is much evidence of a relationship between alterations in the brain's regional cellular energy metabolism and blood flow in schizophrenic. Vascular endothelial growth factor (VEGF) plays a role in the pathogenesis of neuropsychiatric illnesses. So, we compared serum VEGF levels in drug-naïve first-episode psychotic (FEP) and chronically medicated schizophrenic to examine if a correlation existed between VEGF and psychopathological symptoms. The serum VEGF levels were assessed in 46 FEP patients, 47 chronic medicated patients and 50 healthy controls...
April 11, 2018: Psychiatry Research
https://www.readbyqxmd.com/read/29677500/raman-spectral-analysis-for-rapid-screening-of-dengue-infection
#2
T Mahmood, H Nawaz, A Ditta, M I Majeed, M A Hanif, N Rashid, H N Bhatti, H F Nargis, M Saleem, F Bonnier, H J Byrne
Infection with the dengue virus is currently clinically detected according to different biomarkers in human blood plasma, commonly measured by enzyme linked immunosorbent assays, including non-structural proteins (Ns1), immunoglobulin M (IgM) and immunoglobulin G (IgG). However, there is little or no mutual correlation between the biomarkers, as demonstrated in this study by a comparison of their levels in samples from 17 patients. As an alternative, the label free, rapid screening technique, Raman spectroscopy has been used for the characterisation/diagnosis of healthy and dengue infected human blood plasma samples...
April 11, 2018: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/29677452/vitreous-levels-of-luteinizing-hormone-and-vegf-are-strongly-correlated-in-healthy-mammalian-eyes
#3
Tammy Z Movsas, Robert Sigler, Arivalagan Muthusamy
Purpose/Aim: Luteinizing hormone (LH) is known to function as a key regulator of VEGF expression in reproductive organs. In recent years, LH has also been detected in human vitreous and LH receptors have been identified in human retina. This study was aimed to investigate a potential correlation between LH and VEGF levels in healthy mammalian eyes to provide supporting evidence of LH's potential involvement in intraocular VEGF regulation. METHODS: 18 bovine and 30 porcine eyes were procured from an abattoire and VEGF and LH levels were measured in the vitreous extracted from these eyes by commercially-available bovine & porcine ELISA assay kits...
April 20, 2018: Current Eye Research
https://www.readbyqxmd.com/read/29677365/age-related-changes-to-human-tear-composition
#4
Alessandra Micera, Antonio Di Zazzo, Graziana Esposito, Rosa Longo, William Foulsham, Roberto Sacco, Roberto Sgrulletta, Stefano Bonini
Purpose: We characterize age-associated alterations in the expression of inflammatory mediators and tissue remodeling factors in human tears. Methods: A total of 75 consecutive volunteers (32 male/44 female; 19-93 years) underwent clinical assessment of ocular surface status, ocular surface disease index (OSDI) grading and tear sampling. The volunteers were categorized into three groups: young (18-40 years), middle-aged (41-60 years), and old (>60 years). Total protein profiles and chip-based protein array evaluations were conducted to investigate the expression of 60 potential candidates, including pro-/anti-inflammatory mediators and tissue remodeling factors...
April 1, 2018: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29677353/humoral-immune-response-after-intravitreal-but-not-after-subretinal-aav8-in-primates-and-patients
#5
Felix F Reichel, Tobias Peters, Barbara Wilhelm, Martin Biel, Marius Ueffing, Bernd Wissinger, Karl U Bartz-Schmidt, Reinhild Klein, Stylianos Michalakis, M Dominik Fischer
Purpose: To study longitudinal changes of anti-drug antibody (ADA) titers to recombinant adeno-associated virus serotype 8 (rAAV8) capsid epitopes in nonhuman primates (NHP) and patients. Methods: Three groups of six NHP each received subretinal injections (high dose: 1 × 1012 vector genomes [vg], low dose: 1 × 1011 vg, or vehicle only). Four additional animals received intravitreal injections of the high dose (1 × 1012 vg). Three patients received 1 × 1010 vg as subretinal injections...
April 1, 2018: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29677121/prophylactic-neuroprotection-of-total-glucosides-of-paeoniae-radix-alba-against-semen-strychni-induced-neurotoxicity-in-rats-suppressing-oxidative-stress-and-reducing-the-absorption-of-toxic-components
#6
Shujuan Li, Yanjie Chu, Ruowen Zhang, Linjia Sun, Xiaohui Chen
Strychnos alkaloids (SAs) are the main toxic constituents in Semen Strychni, a traditional Chinese medicine, which is known for its fatal neurotoxicity. Hence, the present study was carried out to evaluate the neurotoxicity induced by SAs and the pre-protective effects of the total glucosides of Paeoniae Radix Alba (TGP). An SA brain damage model was firstly established. The neurotoxicity induced by SAs and the pre-protective effects of TGP were confirmed by physical and behavioral testing, biochemical assay, and histological examination...
April 20, 2018: Nutrients
https://www.readbyqxmd.com/read/29676938/the-development-of-a-biotinylated-nad-applied-human-poly-adp-ribose-polymerase-3-parp3-enzymatic-assay
#7
Ming Ji, Liyuan Wang, Nina Xue, Fangfang Lai, Sen Zhang, Jing Jin, Xiaoguang Chen
Poly(ADP-ribose) polymerase 3 (PARP3) is an important member of the PARP family and shares high structural similarities with both PARP1 and PARP2. The biological roles of PARP3 are currently under investigation; however, several key reports indicate the integral roles of PARP3 in DNA damage repair, and thus it has been investigated as a novel target in oncology. It is clear that the identification of selective PARP3 inhibitors would further advance the understanding of the biological roles of PARP3. Herein, we describe a modified PARP3 screening assay using biotinylated NAD+ as the specialized substrate...
April 1, 2018: SLAS Discovery
https://www.readbyqxmd.com/read/29676471/vitamin-d3-regulates-steroidogenesis-in-granulosa-cells-through-amp-activated-protein-kinase-ampk-activation-in-a-mouse-model-of-polycystic-ovary-syndrome
#8
Shabnam Bakhshalizadeh, Fardin Amidi, Reza Shirazi, Maryam Shabani Nashtaei
Polycystic ovary syndrome (PCOS) is an endocrine and metabolic disorder in reproductive-aged women. Hormonal abnormality caused by steroidogenesis disturbances appears to be the main culprit of the clinical picture in PCOS. Vitamin D3 could regulate steroidogenesis in granulosa cells, but the mechanism of action of vitamin D3 on steroidogenesis remains unknown. AMP-activated protein kinase (AMPK) has a modulating role in steroid hormone production. We investigated the effect of vitamin D3 on steroidogenesis in cultured granulosa cells of dehydroepiandrosterone-induced PCOS mice and studied the involvement of AMPK signalling pathway in the current process...
April 20, 2018: Cell Biochemistry and Function
https://www.readbyqxmd.com/read/29676365/rapid-detection-of-respiratory-syncytial-virus-in-community-acquired-lower-respiratory-tract-infections-in-children-by-chromatographic-assay
#9
Surinder Kumar, Bhanu Mehra, Gulshan Rai Sethi, Sanjeev R Saigal
Respiratory syncytial virus (RSV) is the single most important viral agent causing pediatric lower respiratory tract infections (LRTIs) worldwide. To evaluate the role of RSV in pediatric LRTIs, we studied 85 children <2 years of age hospitalized for community-acquired LRTIs. Nasopharyngeal aspirates were obtained on admission for the detection of RSV antigen by immunochromatographic assay. Demographic, clinical, and radiological findings for RSV antigen were compared. Data analysis was performed by Chi-square test...
April 2018: Indian Journal of Pathology & Microbiology
https://www.readbyqxmd.com/read/29676360/detection-of-immunoglobulin-m-and-immunoglobulin-g-antibodies-to-mycoplasma-pneumoniae-in-children-with-community-acquired-lower-respiratory-tract-infections
#10
Surinder Kumar, Indu Bala Garg, Gulshan Rai Sethi, Sanchit Kumar, Sanjeev R Saigal
Context: Mycoplasma pneumoniae (M. pneumoniae) causes up to 40% of community-acquired pneumonia in children. It is impossible to identify M. pneumoniae infection on the basis of clinical signs, symptoms, and radiological features. Therefore, correct etiological diagnosis strongly depends on laboratory diagnosis. Aims: This study aims to investigate the role of M. pneumonia e in pediatric lower respiratory tract infections (LRTIs) employing enzyme-linked immunosorbent assays (ELISA) and particle agglutination (PA) test...
April 2018: Indian Journal of Pathology & Microbiology
https://www.readbyqxmd.com/read/29676359/molecular-genetics-of-bcr-abl1-negative-myeloproliferative-neoplasms-in-india
#11
Nikhil Rabade, P G Subramanian, Rohan Kodgule, Goutham Raval, Swapnali Joshi, Shruti Chaudhary, Russel Mascarenhas, Prashant Tembhare, Sumeet Gujral, Nikhil Patkar
Introduction: Over the past decade, we have moved on from a predominantly morphological and clinical classification of myeloproliferative neoplasms (MPN) to a more evolved classification that accounts for the molecular heterogeneity that is unique to this subgroup of hematological malignancies. This usually incorporates mutations in Janus kinase 2 (JAK2), MPL, and calreticulin (CALR) genes. In this manuscript, we report the frequency of these mutations in a cohort of Indian patients at a tertiary cancer center...
April 2018: Indian Journal of Pathology & Microbiology
https://www.readbyqxmd.com/read/29676236/monoamine-involvement-in-the-antidepressant-like-effect-of-%C3%AE-caryophyllene
#12
Danillo Ramos Oliveira, Dayane Moreira Silva, Iziara Ferreira Florentino, Adriane de Brito, James Oluwagbamigbe Fajemiroye, Daiany Priscilla Bueno Silva, Fabio da Rocha, Elson Alves Costa, Pablinny Galdino De Carvalho
This study was conducted to evaluate the antidepressant-like activity of sesquiterpene compound β-caryophyllene (BCP) for the possible contribution of the monoamine and hippocampal levels of brain-derived neurotrophic factor (BDNF). Male albino Swiss mice were subjected to the forced swimming test after acute treatment and to the tail suspension test after repeated treatment. Hippocampal levels of BDNF were assayed by enzyme-linked immunosorbent assay. The anti-immobility effect of BCP was reverted by pretreatment with inhibitor of catecholamines α-methyl-p-tyrosine (100 mg/kg, i...
April 20, 2018: CNS & Neurological Disorders Drug Targets
https://www.readbyqxmd.com/read/29676105/-effects-of-tongbi-capsule-on-joint-lesions-in-rabbits-with-rheumatoid-arthritis
#13
Qing-Yong Cao, Pei-Yuan Wang, Qiao-Yun Wang, Feng Zhao, Ke-Zhan Wang, Jun-Qi Liu, Tong-Shen Liu, Yuan-Fang Wang, Yong-Jun Liu
The purpose of this experiment is to observe the effects of Tongbi capsule on joint lesions in rabbit with rheumatoid arthritis induced by ovalbumin and explore the mechanism in order to provide reference for clinical application of Tongbi capsule. Rheumatoid arthritis in rabbits was induced by subcutaneous injection of emulsions of ovalbumin and Freund's complete adjuvant and intra articular injection of ovalbumin. After successful modeling, 30 New Zealand rabbits with arthritis were randomly divided into model control group, the high, medium and low dose groups of Tongbi capsule (90, 45, 22...
March 2018: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29676026/selective-inversion-recovery-quantitative-magnetization-transfer-brain-mri-at-7t-clinical-and-postmortem-validation-in-multiple-sclerosis
#14
Francesca Bagnato, Simon Hametner, Giulia Franco, Siddharama Pawate, Subramaniam Sriram, Hans Lassmann, John Gore, Seth E Smith, Richard Dortch
BACKGROUND AND PURPOSE: An imaging biomarker of myelin integrity is an unmet need in multiple sclerosis (MS). Selective inversion recovery (SIR) quantitative magnetization transfer imaging (qMT) provides assays of myelin content in the human brain. We previously translated the SIR method to 7T and incorporated a rapid turbo field echo (TFE) readout for whole-brain imaging within clinically acceptable scan times. We herein provide histological validation and test in vivo feasibility and applicability of the SIR-TFE protocol in MS...
April 19, 2018: Journal of Neuroimaging: Official Journal of the American Society of Neuroimaging
https://www.readbyqxmd.com/read/29676021/detection-of-alpha-synuclein-conformational-variants-from-gastro-intestinal-biopsy-tissue-as-a-potential-biomarker-for-parkinson-s-disease
#15
Claudio Ruffmann, Nora Bengoa-Vergniory, Ilaria Poggiolini, Diane Ritchie, Michele T Hu, Javier Alegre-Abarrategui, Laura Parkkinen
AIMS: Gastrointestinal (GI) α-synuclein (aSyn) detection as a potential biomarker of Parkinson's disease (PD) is challenged by conflicting results of recent studies. To increase sensitivity and specificity, we applied three techniques to detect different conformations of aSyn in GI biopsies obtained from a longitudinal, clinically well-characterized cohort of PD patients and healthy controls (HC). METHODS: With immunohistochemistry (IHC), we used antibodies reactive for total, phosphorylated and oligomeric aSyn; with aSyn proximity ligation assay (AS-PLA), we targeted oligomeric aSyn species specifically; and with paraffin-embedded tissue blot (AS-PET-blot) we aimed to detect fibrillary, synaptic aSyn...
April 19, 2018: Neuropathology and Applied Neurobiology
https://www.readbyqxmd.com/read/29675931/efficacy-studies-of-sclerotium-rolfsii-lectin-on-breast-cancer-using-nod-scid-mouse-model
#16
Prajna Hegde, Jagadeesh Narasimhappagari, Bale M Swamy, Shashikala R Inamdar
Expression of altered glycans like TF, Tn and sTn antigens has been observed in a number of carcinomas which are targeted in cancer therapy. Sclerotium rolfsii lectin (SRL) is known to recognize TF and its substituted forms. Clinical potential of SRL has been demonstrated by studying its interaction with different types of cancer cells. Here we report, in vitro studies of SRL on breast cancer MDA-MB-468 cells and in vivo studies with MCF-7 xenografts. In vitro growth inhibitory studies of SRL on metastatic triple negative breast cancer MDA-MB-468 cells was performed by MTT assay, flow cytometry, adhesion and CAM assay...
April 20, 2018: Chemical Biology & Drug Design
https://www.readbyqxmd.com/read/29675789/experimental-platform-utilising-melting-curve-technology-for-detection-of-mutations-in-mycobacterium-tuberculosis-isolates
#17
Agnieszka Broda, Vlad Nikolayevskyy, Nicki Casali, Huma Khan, Richard Bowker, Gemma Blackwell, Bhakti Patel, James Hume, Waqar Hussain, Francis Drobniewski
Tuberculosis (TB) remains one of the most deadly infections with approximately a quarter of cases not being identified and/or treated mainly due to a lack of resources. Rapid detection of TB or drug-resistant TB enables timely adequate treatment and is a cornerstone of effective TB management. We evaluated the analytical performance of a single-tube assay for multidrug-resistant TB (MDR-TB) on an experimental platform utilising RT-PCR and melting curve analysis that could potentially be operated as a point-of-care (PoC) test in resource-constrained settings with a high burden of TB...
April 20, 2018: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/29675682/development-of-nanoparticle-assisted-pcr-assay-in-the-rapid-detection-of-brain-eating-amoebae
#18
Shobana Gabriel, Abdul Khaliq Rasheed, Ruqaiyyah Siddiqui, Jimmy Nelson Appaturi, Leo Bey Fen, Naveed Ahmed Khan
Brain-eating amoebae (Acanthamoeba spp., Balamuthia mandrillaris, Naegleria fowleri) have gained increasing attention owing to their capacity to produce severe human and animal infections involving the brain. Early detection is a pre-requisite in successful prognosis. Here, we developed a nanoPCR assay for the rapid detection of brain-eating amoebae using various nanoparticles. Graphene oxide, copper and alumina nanoparticles used in this study were characterized using Raman spectroscopy measurements through excitation with a He-Ne laser, while powder X-ray diffraction patterns were taken on a PANanalytical, X'Pert HighScore diffractometer and the morphology of the materials was confirmed using high-resolution transmission electron microscopy (HRTEM)...
April 20, 2018: Parasitology Research
https://www.readbyqxmd.com/read/29675559/truncated-aptamers-for-total-and-glycated-hemoglobin-and-their-integration-into-a-graphene-oxide-based-fluorometric-method-for-high-throughput-screening-for-diabetes
#19
Abrar Yousef Almusharraf, Shimaa Eissa, Mohammed Zourob
The authors describe the identification of an effective binding region of aptamers against glycated (HbA1c) and total haemoglobin (tHb) by using a fluorometric assay. Truncation of the originally selected aptamers from 60 to 46 and 34 nucleotides for HbA1c and tHb, respectively, enhances the affinity for their targets. Moreover, shortening the aptamer sequences leads to a better conformational change after target binding which enabled the integration of the aptamers in a graphene oxide (GO)-based fluorometric assay...
April 19, 2018: Mikrochimica Acta
https://www.readbyqxmd.com/read/29675287/towards-a-dual-lateral-flow-nanobiosensor-for-simultaneous-detection-of-virus-genotype-specific-pcr-products
#20
Dimitra K Toubanaki, Evdokia Karagouni
Nervous necrosis virus (nodavirus) has been responsible for mass mortalities in aquaculture industry worldwide, with great economic and environmental impact. A rapid low-cost test to identify nodavirus genotype could have important benefits for vaccine and diagnostic applications in small- and medium-scale laboratories in both academia and fish farming industry. A dual lateral flow biosensor for simultaneous detection of the most prevalent nodavirus genotypes (RGNNV and SJNNV) was developed and optimized. The dual biosensor consisted of two antibody-based test zones, indicative of each genotype, and a control zone...
2018: Journal of Analytical Methods in Chemistry
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